CN103843807B - A kind of method of Prochloraz prevention red bayberry blight - Google Patents
A kind of method of Prochloraz prevention red bayberry blight Download PDFInfo
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- CN103843807B CN103843807B CN201410017441.2A CN201410017441A CN103843807B CN 103843807 B CN103843807 B CN 103843807B CN 201410017441 A CN201410017441 A CN 201410017441A CN 103843807 B CN103843807 B CN 103843807B
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Abstract
The invention provides a kind of method of prevention or control red bayberry blight, it is characterized in that, the method comprises: provide mass percent be 1% Prochloraz reagent; Smear the wound of strawberry tree, allowing the amount of Prochloraz on every square centimeter wound is 1-10 milligram.Effectively can be prevented and treated the blight of red bayberry by method of the present invention, particularly infect the blight that wound causes.
Description
Technical field
The invention belongs to field of plant disease control, especially, relate to reagent and the method for control red bayberry blight.
Background
Red bayberry (Myrica rubra Sieb.Et Zucc.) is the distinctive rare fruit of south China, fruit sweet and sour palatability, unique flavor, at home and abroad enjoy high reputation, because of its significant economy and zoology benefit, become Zhejiang Province's main fruit kind, it is the first that its output value has been sure to occupy all kinds of fruit.Red bayberry blight is newfound a kind of disease in recent years, their early stage, and the tender tip of red bayberry part dries up, with aggravation, the tender tip quantity that dries up increases gradually and spreads to full tree, and after morbidity, the whole strain of 3-5 is dead.Red bayberry blight is rapid spread trend in red bayberry main producing region, Zhejiang Province, has a strong impact on the sustainable development of red bayberry industry.Red bayberry blight pathogen is Pestalotiopsis versicolor (Pestalotiopsis versicolor) and Pestalotiopsis microspora (P.microspora).
This just needs the reagent and the method that provide a kind of effective control or prevention red bayberry blight.
Summary of the invention
Orchard worker prunes strawberry tree in the capital in February every year from October to next year, especially July in summer red bayberry can carry out after having gathered in the crops shearing big branch complete tree low body, this just cause strawberry tree body produce much prune wound.The discovery that our research group is surprised, wilting germ (the spore of Pestalotiopsis versicolor (Pestalotiopsis versicolor) and Pestalotiopsis microspora (P.microspora), mycelium) main path that invades red bayberry is wound, but not epidermis pore or other approach.Field is collected conidium and is found; two conidiums release peak periods by the end of September except arriving by the end of April by the end of June and arriving by the end of August; have conidium to discharge in other week year, and spore can be sprouted at 5-35 DEG C, this just illustrates the protection of strawberry tree pruning wound extremely important.
On the one hand, the invention provides a kind of method of prevention or control red bayberry blight, it is characterized in that: provide mass percent be 1% Prochloraz reagent, the wound of strawberry tree is smeared, allows active drug concentration on the wound of every square centimeter for 1-10 milligram.
Preferably, the collocation method of Prochloraz reagent of described 1% is as follows: get the former medicine of 5g Prochloraz, be slowly dissolved in the 95g n-butanol solvent of 50 DEG C; And then n-butanol solvent adds 601 emulsifier of 100g and stirring and evenly mixing gently, after being uniformly dispersed, with the dilution of 300g water emulsion, obtains the Prochloraz reagent of 1% concentration.
Preferably, described wound is that pruning fruit trees formed.
Here wound also can make any damage fruit tree be formed, such as, bitten by animals, by the wound that the paper slip that fractures is formed.N-butanol mentioned here can also be replaced by ethanol, propyl alcohol or other alcohol, but adopts the preventive effect of n-butanol best, and the alcohols organic substance of section chain can be used as solvent.The not special requirement of emulsifier, general emulsifier can.Water emulsion is also conventional reagent.
Beneficial effect
Adopt reagent of the present invention to carry out blight control to red bayberry wound, the control efficiency that quite traditional whole tree carries out spraying and adopt the aqueous solution to smear the method for wound all significantly improves.
Embodiment
experiment 1: semar technique is to the control efficiency of red bayberry blight
1 materials and methods
The preparation of 1.1 Wound protective agents
The former medicine of 5g Prochloraz (Jiangsu Hui Feng agrochemical Co., Ltd) is slowly dissolved in the 95g n-butanol solvent of 50 DEG C; then at 601 emulsifier (Electro-group compounding chemicals Chemical Industry Co., Ltd. of Hangzhou) of 100g and stirring and evenly mixing gently; wait to be uniformly dispersed; with 300g water emulsion (Ke Haikaimei chemical company) dilution, the Wound protective agent of 1% concentration Prochloraz (MX) has been prepared.
1.2 wilting germ conidia solution preparations
Wilting germ Pestalotiopsis versicolor XJ27 and Pestalotiopsis microspora YS26 cultivates on PDA flat board, and dark 25 DEG C of 2000lux12h illumination/12h cultivates 5 days, then carefully washes down with aqua sterilisa, is made into 2 × 10
5individual mL
-1the conidial suspension of mixing, inoculate for subsequent use.
1.3 red bayberry blight prevention experiments
The east of life in 2 years chief container seedling is selected to be placed in greenhouse, temperature 25 DEG C ± 2 DEG C, relative moisture 85% ± 5%.Select to have the spray of life in 1 year of branch, one of them spray cut section an about 10cm, with banister brush carefully MX uniform application to (content of the active drug of each wound is 10 milligrams/every square centimeter) on the wound of spray.Arrange and contrast not daubing medicament.Then draw the germ spore suspension of 20 μ l at the contrast branch wound pipettor of daubing medicament or not daubing medicament, absorbent cotton water suction covers, and preservative film is wound around moisturizing.Often process arranges 30 repetitions.
Inoculate branch's spray incidence of disease that investigation in latter 1 month is adjacent, the total branch number in the incidence of disease (%)=morbidity branch quantity × 100/.Preventive effect (%)=(the contrast incidence of disease-process incidence of disease) × 100/ contrast incidence of disease.
Preventive effect result
The medicine control efficiency of table 1 semar technique
In addition, according to above-mentioned same method, valid density different to Prochloraz (MX) is respectively tested, and result is as follows
The control efficiency of the effective dose that table 2 Prochloraz (MX) is different
Experiment 2: solution smears prevention and controls.
Preparation Prochloraz active ingredient is the aqueous solution of 100 milligrams every milliliter, selects the east of life in 2 years chief container seedling to be placed in greenhouse, temperature 25 DEG C ± 2 DEG C, relative moisture 85% ± 5%.Select the spray having life in 1 year of branch, one of them spray is cut and cuts about 10cm, carefully MX dissolution homogeneity is smeared to (medicament of each wound is approximately 100 microlitres, and active drug is 10 milligrams every square centimeter) on the wound of spray with liquid-transfering gun, arrange and contrast not daubing medicament.Then draw the germ spore suspension of 20 μ l at the contrast branch wound pipettor of daubing medicament or not daubing medicament, absorbent cotton water suction covers, and preservative film is wound around moisturizing.Often process arranges 30 repetitions.
Result
The control efficiency that the table 2 Prochloraz aqueous solution is smeared
Examples of implementation 3:
With examples of implementation 2 difference be: adopt and entirely set the spray Prochloraz solution (also comprising wound) that the method for spraying carries out the aqueous solution of concentration 100 milligrams every milliliter.The incidence of disease is 64%, and preventive effect is 37.2%.
As apparent from above experiment can, adopt colloid of the present invention to smear wound, significantly improve the preventive effect of the blight of red bayberry, the incidence of disease significantly reduces, and the concentration of active drug is often at least 1 milligram at the wound of square centimeter.Spray-on process is carried out to whole tree and the control efficiency that adopts the aqueous solution to smear the method for wound all significantly improves than conventional.
Claims (2)
1. a method for prevention or control red bayberry blight, is characterized in that: on the wound of red bayberry, smear the Prochloraz reagent of 1%, the collocation method of this reagent is as follows: get the former medicine of 5g Prochloraz, is slowly dissolved in the 95g n-butanol solvent of 50 DEG C; And then add 601 emulsifier of 100g and stirring and evenly mixing gently to n-butanol solvent, after being uniformly dispersed, with the dilution of 300g water emulsion, obtain the Prochloraz reagent of 1% concentration; Wherein, allowing the amount of Prochloraz on the wound of every square centimeter is 1-10 milligram.
2. for preventing a protection reagent for red bayberry blight, it is characterized in that: this protection reagent comprises the Prochloraz of 1%, the collocation method of the Prochloraz reagent of 1% is as follows: get the former medicine of 5g Prochloraz, be slowly dissolved in the 95g n-butanol solvent of 50 DEG C; And then add 601 emulsifier of 100g and stirring and evenly mixing gently to n-butanol solvent, after being uniformly dispersed, with the dilution of 300g water emulsion, obtain the Prochloraz reagent of 1% concentration; This reagent is applied on the wound of red bayberry.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410017441.2A CN103843807B (en) | 2014-01-15 | 2014-01-15 | A kind of method of Prochloraz prevention red bayberry blight |
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| CN201410017441.2A CN103843807B (en) | 2014-01-15 | 2014-01-15 | A kind of method of Prochloraz prevention red bayberry blight |
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| CN103843807A CN103843807A (en) | 2014-06-11 |
| CN103843807B true CN103843807B (en) | 2015-08-12 |
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| CN106636301A (en) * | 2016-09-29 | 2017-05-10 | 贵州医科大学 | Method for selecting chemical agent for preventing and treating leave diseases of alpinia zerumbet |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010123849A1 (en) * | 2009-04-20 | 2010-10-28 | Valent Biosciences Corporation | Fungicidal composition |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010123849A1 (en) * | 2009-04-20 | 2010-10-28 | Valent Biosciences Corporation | Fungicidal composition |
Non-Patent Citations (3)
| Title |
|---|
| 几种农用杀菌剂对杨梅控病保鲜的药效研究;郭泗明;《现代农业科技》;20111231(第17期);第318,320页 * |
| 十种杀菌剂对杨梅凋萎病的药效评价;任海英 等;《果树学报》;20131231;第30卷(第5期);第848-853页 * |
| 杨梅突发性枝叶凋萎病发病调查与病原接种研究;求盈盈 等;《浙江农业科学》;20111231(第1期);第98-100页 * |
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