CN103798243B - A kind of method of Difenoconazole prevention or control red bayberry blight - Google Patents
A kind of method of Difenoconazole prevention or control red bayberry blight Download PDFInfo
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- CN103798243B CN103798243B CN201410017807.6A CN201410017807A CN103798243B CN 103798243 B CN103798243 B CN 103798243B CN 201410017807 A CN201410017807 A CN 201410017807A CN 103798243 B CN103798243 B CN 103798243B
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Abstract
The invention provides a kind of method of prevention or control red bayberry blight, it is characterized in that, the method comprises: provide mass percent be 1% Difenoconazole reagent; Smear the wound of strawberry tree, allowing the amount of Difenoconazole on every square centimeter wound is 1-10 milligram.Effectively can be prevented and treated the blight of red bayberry by method of the present invention, particularly infect the blight that wound causes.
Description
Technical field
The invention belongs to field of plant disease control, especially, relate to reagent and the method for control red bayberry blight.
Background
Red bayberry (Myrica rubra Sieb.Et Zucc.) is the distinctive rare fruit of south China, fruit sweet and sour palatability, unique flavor, at home and abroad enjoy high reputation, because of its significant economy and zoology benefit, become Zhejiang Province's main fruit kind, it is the first that its output value has been sure to occupy all kinds of fruit.Red bayberry blight is newfound a kind of disease in recent years, their early stage, and the tender tip of red bayberry part dries up, with aggravation, the tender tip quantity that dries up increases gradually and spreads to full tree, and after morbidity, the whole strain of 3-5 is dead.Red bayberry blight is rapid spread trend in red bayberry main producing region, Zhejiang Province, has a strong impact on the sustainable development of red bayberry industry.Red bayberry blight pathogen is Pestalotiopsis versicolor (Pestalotiopsis versicolor) and Pestalotiopsis microspora (P.microspora).
This just needs the reagent and the method that provide a kind of effective control or prevention red bayberry blight.
Summary of the invention
Orchard worker prunes strawberry tree in the capital in February every year from October to next year, especially July in summer red bayberry can carry out after having gathered in the crops shearing big branch complete tree low body, this just cause strawberry tree body produce much prune wound.We find in research group's previous work, and the main path that wilting germ invades red bayberry is wound, but not epidermis pore or other approach.Field is collected conidium and is found; two conidiums release peak periods by the end of September except arriving by the end of April by the end of June and arriving by the end of August; have conidium to discharge in other week year, and spore can be sprouted at 5-35 DEG C, this just illustrates the protection of strawberry tree pruning wound extremely important.
On the one hand, the invention provides a kind of method of prevention or control red bayberry blight, it is characterized in that: smear the wound of strawberry tree, allowing the amount of Difenoconazole on every square centimeter wound is 1-10 milligram.
Wherein, have preferred, Difenoconazole is included in reagent, and the percentage of described Difenoconazole is 1%.
Preferably, the collocation method of Difenoconazole reagent of described 1% is as follows: get 5g difenoconazole technical material, be slowly dissolved in the 95g n-butanol solvent of 50 DEG C; Then add 601 emulsifier of 100g and stirring and evenly mixing gently, wait to be uniformly dispersed; With the dilution of 300g water emulsion, obtain Difenoconazole (BM) the emulsion state agricultural chemicals reagent that mass percent is 1%.
Preferably, described wound is that pruning fruit trees formed.
Here wound also can make any damage fruit tree be formed, such as, bitten by animals, by the wound that the paper slip that fractures is formed.N-butanol mentioned here can also be replaced by ethanol, propyl alcohol or other alcohol, but adopts the preventive effect of n-butanol best, and the alcohols organic substance of section chain can be used as solvent.The not special requirement of emulsifier, general emulsifier can.Water emulsion is also conventional reagent.
Using Difenoconazole as smearing reagent in control or a purposes of preventing red bayberry blight, wherein smearing reagent is spread upon on the wound of red bayberry.Preferably, this wound is caused by pruning.Preferably, allowing the amount of Difenoconazole on every square centimeter wound is 1-10 milligram.
Beneficial effect
Adopt reagent of the present invention to carry out blight control to red bayberry wound, the control efficiency that quite traditional whole tree carries out spraying and adopt the aqueous solution to smear the method for wound all significantly improves.
Embodiment
experiment 1: semar technique is to the control efficiency of red bayberry blight
1 materials and methods
The preparation of 1.1 Wound protective agents
5g difenoconazole technical material (Jiangsu Yunnong Chemical Co., Ltd.), slowly be dissolved in the 95g n-butanol solvent of 50 DEG C, in n-butanol solvent, then add 601 emulsifier (Electro-group compounding chemicals Chemical Industry Co., Ltd. of Hangzhou) of 100g and stirring and evenly mixing gently.Wait to be uniformly dispersed, with 300g water emulsion (Ke Haikaimei chemical company) dilution, obtain the Wound protective agent of the Difenoconazole (BM) of 1% concentration.
1.2 wilting germ conidia solution preparations
Wilting germ Pestalotiopsis versicolor XJ27 and Pestalotiopsis microspora YS26 cultivates on PDA flat board, and dark 25 DEG C of 2000lux12h illumination/12h cultivates 5 days, then carefully washes down with aqua sterilisa, is made into 2 × 10
5individual mL
-1the conidial suspension of mixing, inoculate for subsequent use.
1.3 red bayberry blight prevention experiments
The east of life in 2 years chief container seedling is selected to be placed in greenhouse, temperature 25 DEG C ± 2 DEG C, relative moisture 85% ± 5%.Select to have the spray of life in 1 year of branch, one of them spray cut and cuts an about 10cm, with banister brush carefully BM uniform application to (content of the effect medicine of each wound be 10 in the least gram/cm) on each wound of spray.Arrange and contrast not daubing medicament.Then draw the germ spore suspension of 20 μ l at the contrast branch wound pipettor of daubing medicament or not daubing medicament, absorbent cotton water suction covers, and preservative film is wound around moisturizing.Often process arranges 30 repetitions.
Inoculate branch's spray incidence of disease that investigation in latter 1 month is adjacent, the total branch number in the incidence of disease=morbidity branch quantity × 100/.Preventive effect (%)=(the contrast incidence of disease-process incidence of disease) × 100/ contrast incidence of disease.
2 results
The medicine control efficiency of table 1 semar technique
In addition, according to above-mentioned same method, the valid density that p-phenylene's methyl cyclic-azole (BM) is different is respectively tested, and result is as follows:
The valid density control efficiency that table 2 Difenoconazole (BM) is different
Experiment 2: solution smears prevention and controls.
The aqueous solution of the concentration 100 milligrams every milliliter of Difenoconazole, selects the east of life in 2 years chief container seedling to be placed in greenhouse, temperature 25 DEG C ± 2 DEG C, relative moisture 85% ± 5%.Select the spray having life in 1 year of branch, one of them spray is cut and cuts about 10cm, carefully BM dissolution homogeneity is smeared to (medicament of each wound is approximately 100 microlitres, and active drug is 10 millis gram/cm) on each wound of spray with liquid-transfering gun, arrange and contrast not daubing medicament.Then draw the germ spore suspension of 20 μ l at the contrast branch wound pipettor of daubing medicament or not daubing medicament, absorbent cotton water suction covers, and preservative film is wound around moisturizing.Often process arranges 30 repetitions.
Result
The medicine control efficiency that table 3 aqueous solution is smeared
Examples of implementation 3:
With examples of implementation 2 difference be: adopt and entirely set the aqueous solution (also comprising wound) that the method for spraying carries out the Difenoconazole of spray concentration 100 milligrams every milliliter.Result is: the incidence of disease is 65%, and preventive effect is 34.6%.
As apparent from above experiment can, adopt colloid of the present invention to smear wound, to the remarkable height of the preventive effect of the blight of red bayberry, the incidence of disease significantly reduces, and the concentration of active drug is often at least 1 milligram at the wound of square centimeter.Spray-on process is carried out to whole tree and the control efficiency that adopts the aqueous solution to smear the method for wound all significantly improves than conventional.
Claims (2)
1. a method for prevention or control red bayberry blight, it is characterized in that, the method comprises: provide mass percent be 1% Difenoconazole reagent; Smear the wound of strawberry tree, allowing the amount of Difenoconazole on the wound of every square centimeter is 1-10 milligram; Wherein, the method preparing the Difenoconazole reagent of 1% comprises: get 5 grams of Difenoconazoles, be slowly dissolved in the n-butanol solvent of the 95g of 50 DEG C; Then in butanol solution, 601 emulsifier of 100g are added and stirring and evenly mixing gently, after being uniformly dispersed; With the dilution of 300g water emulsion, obtain the Difenoconazole agricultural chemicals reagent that mass percent is 1%; Described wound prunes strawberry tree to be formed.
2. one kind using Difenoconazole as smearing the purposes of reagent in control or prevention red bayberry blight, wherein smearing reagent is spread upon on the wound of red bayberry, allowing the amount of Difenoconazole on every square centimeter wound is 1-10 milligram, and wherein, the mass percent concentration of Difenoconazole reagent is 1%; Wherein, the method preparing the Difenoconazole reagent of 1% comprises: get 5 grams of Difenoconazoles, be slowly dissolved in the n-butanol solvent of the 95g of 50 DEG C; Then in butanol solution, 601 emulsifier of 100g are added and stirring and evenly mixing gently, after being uniformly dispersed; With the dilution of 300g water emulsion, obtain the Difenoconazole agricultural chemicals reagent that mass percent is 1%, described wound prunes strawberry tree to be formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410017807.6A CN103798243B (en) | 2014-01-15 | 2014-01-15 | A kind of method of Difenoconazole prevention or control red bayberry blight |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410017807.6A CN103798243B (en) | 2014-01-15 | 2014-01-15 | A kind of method of Difenoconazole prevention or control red bayberry blight |
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| Publication Number | Publication Date |
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| CN103798243A CN103798243A (en) | 2014-05-21 |
| CN103798243B true CN103798243B (en) | 2015-09-02 |
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| CN115280947A (en) * | 2022-08-11 | 2022-11-04 | 泰顺县白巢洋家庭农场有限公司 | Method for preventing and treating waxberry wilting disease |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5266585A (en) * | 1981-05-12 | 1993-11-30 | Ciba-Geigy Corporation | Arylphenyl ether derivatives, compositions containing these compounds and use thereof |
| CN101810184A (en) * | 2009-11-25 | 2010-08-25 | 陕西永泰生物工程有限责任公司 | Plant wound healing protective agent and preparation method thereof |
| CN102334514A (en) * | 2011-07-19 | 2012-02-01 | 陕西喜嘉旺生物科技有限责任公司 | Tree wound healing protective agent and preparation method thereof |
-
2014
- 2014-01-15 CN CN201410017807.6A patent/CN103798243B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5266585A (en) * | 1981-05-12 | 1993-11-30 | Ciba-Geigy Corporation | Arylphenyl ether derivatives, compositions containing these compounds and use thereof |
| CN101810184A (en) * | 2009-11-25 | 2010-08-25 | 陕西永泰生物工程有限责任公司 | Plant wound healing protective agent and preparation method thereof |
| CN102334514A (en) * | 2011-07-19 | 2012-02-01 | 陕西喜嘉旺生物科技有限责任公司 | Tree wound healing protective agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| "十种杀菌剂对杨梅凋萎病的药效评价";任海英等;《果树学报》;20130910;第30卷(第5期);第848-853页 * |
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