CN112293417A

CN112293417A - Application of 6-methylcoumarin in aphicide and aphicide thereof

Info

Publication number: CN112293417A
Application number: CN202011217173.0A
Authority: CN
Inventors: 丁伟; 万凤琳; 周红; 简渝凡; 郭富友; 苏燕珊
Original assignee: Southwest University
Current assignee: Southwest University
Priority date: 2020-11-04
Filing date: 2020-11-04
Publication date: 2021-02-02

Abstract

The invention discloses application of 6-methylcoumarin in an aphicide and the aphicide, belongs to the field of pesticides, and relates to the field of pesticides, wherein the 6-methylcoumarin aphicide is used for killing aphids of phylloxera, particularly cotton aphids, green peach aphids, radish aphids, cabbage aphids or melon aphids, and comprises 6-methylcoumarin, a solvent, an emulsifier and a solubilizer. Compared with other commercial aphicides, the aphicide containing 6-methylcoumarin prepared by the invention has comparable aphid killing effect, has wide development prospect and application value, and is an effective and feasible novel aphicide. Meanwhile, the 6-methylcoumarin has wide source and simple preparation process, is favorable for reducing the cost of the aphicide and is favorable for popularization.

Description

Application of 6-methylcoumarin in aphicide and aphicide thereof

Technical Field

The invention relates to the field of pesticides, and particularly relates to application of 6-methylcoumarin in an aphicide and the aphicide.

Background

The aphid is commonly called as greate insect or honey worm, belonging to Hemiptera (originally homoptera Hemiptera), including Adelgoidea of the Myzus globulus superfamily and Aphidoidea of the Aphis globulus superfamily. Aphids are mainly distributed in temperate regions and subtropical regions of the northern hemisphere, the distribution of the tropical regions is very small, and more than 4700 aphids in the general class are known in the world at present. Aphids vary in size, ranging in length from one to ten millimeters. Aphids feed on plant sap and, at the same time, the peritoneal tube discharges a rapidly hardening defense solution, which not only hinders plant growth, forms galls, spreads viruses, but also causes flower, leaf and bud deformities. Is a big plant pest. The life history is complex, most types of the pests are oligofood or monofood, the few types of the pests are polyphagia, and part of types of the pests are important pests of economic plants such as grains, cotton, oil, hemp, tea, sugar, vegetables, tobacco, fruits, medicines, trees and the like. And as the migration flight and diffusion are carried out to search for the host plants, the host plants need to be repeatedly transferred and tasted, so that a plurality of plant virus diseases can be transmitted, and the crop is more damaged. About 250 of them are harmful pests to agriculture, forestry and gardening. The phytophagous aphids which are seriously harmful in China comprise important pests such as sitobion avenae, schizaphis graminifolia, cotton aphids, green peach aphids, radish aphids and the like. The aphid pest has strong reproductive capacity, outstanding generation overlapping phenomenon, parthenogenesis and strong adaptability, and is one of the most difficult pest control.

6-Methylcoumarin (6-Methylocumarin), also known as 6-methyl-2H-1-benzopyran-2-one; 6-methylbenzopyrone; 6-Methyloxazolone. The boiling point is 303 ℃ (99.66kPa), the melting point is 73-76 ℃, and the flash point is 67.2 ℃. Soluble in benzene, hot ethanol and non-volatile oils, and insoluble in hot water. The essence is distributed in higher plants, particularly Rutaceae and umbelliferae, also widely exists in leguminosae, orchidaceae and melilotaceae, is white crystalline solid in appearance, has strong coconut aroma and sweet smell, is an important aroma, is commonly used as an aroma fixative, a deodorizer, and is prepared into perfume and spice, is mainly used for preparing coconut, vanilla, caramel and other types of essence, is also used as an intermediate, is used for preparing organic synthesis and cosmetics, is also used as an aroma enhancer for rubber and plastic products, and can also be used as a polishing agent and a brightening agent for processing metal surfaces.

At present, aphicides such as imidacloprid and aphicide are commonly used in agriculture to kill aphids, and the aphids are resistant to drugs after the aphicides are used for a long time, so that a novel aphicide is urgently needed to be found to kill the aphids.

Disclosure of Invention

In view of the above, the present invention aims to provide an application of 6-methylcoumarin in an aphicide and an aphicide thereof.

The invention solves the technical problems by the following technical means:

the invention discloses application of 6-methylcoumarin in aphid killing.

Further, the 6-methylcoumarin aphicide is used for killing aphids of the aphis leafhopper class.

Further, the aphicide mainly has a high-efficiency killing effect on cotton aphids, green peach aphids, radish aphids, cabbage aphids or melon aphids in the aphid-type aphids.

The invention also discloses a composition of the 6-methylcoumarin aphicide, wherein the aphicide comprises 6-methylcoumarin, a solvent, an emulsifier and a solubilizer.

Further, the aphicide consists of the following components in parts by weight: 5-15 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 5-15 parts of emulsifier.

Further, the aphicide consists of the following components in parts by weight:

5 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 15 parts of emulsifier;

or 10 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 10 parts of emulsifier;

or 15 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 5 parts of emulsifier.

Further, the solvent is N-methyl pyrrolidone.

Of course, the solvent may be acetone or chloroform.

Further, the emulsifier is span and tween, and the emulsifier can be tween 85, tween 20, tween 60 or tween 80.

Of course, the emulsifier can also be selected from nonionic surfactants of the ethylene glycol ester type, propylene glycol ester type or polyethylene glycol type.

Further, the solubilizer is turpentine.

The invention also discloses a preparation method of the aphicide, which comprises the following steps: dissolving 6-methylcoumarin in solvent, adding emulsifier and solubilizer, and mixing.

Further, the aphicide needs to be diluted for use, and is used for preventing and treating aphids in a spraying mode.

Has the advantages that:

1. 6-methylcoumarin has aphid killing effect, the death rate of aphids reaches 100% when the concentration is 2mg/mL, and the contact activity is obvious.

2. The 6-methylcoumarin has good control effect after being prepared into the aphicide, the control effect reaches 76.28 percent in 15 days, and the systemic activity is remarkable.

3. Compared with other commercial aphicides, the aphicide containing 6-methylcoumarin has comparable aphid killing effect, especially has obvious contact killing activity on cotton aphids, has wide development prospect and application value, and is an effective and feasible novel aphicide.

4. The 6-methylcoumarin has wide source and simple preparation process, is beneficial to reducing the cost of the aphicide and is beneficial to popularization. Meanwhile, the aphicide prepared from the 6-methylcoumarin is safe to human and livestock, environment-friendly, belongs to a novel green pesticide, and has profound significance for long-term development of ecological environment, human health, full utilization of environmental resources and comprehensive treatment of diseases.

Drawings

FIG. 1: plant pictures after 50-fold, 100-fold, 200-fold and 400-fold dilution of 6-methylcoumarin in imidacloprid and aphicide 1;

FIG. 2: diluting 6-methylcoumarin in imidacloprid and aphicide 1 by 50 times, 100 times, 200 times, 400 times and 800 times, and then processing front and back leaf images of plants;

FIG. 3: and (3) diluting 6-methylcoumarin in imidacloprid and aphicide 1 by 50 times, and then processing the plant pictures.

Detailed Description

The present invention will be described in detail with reference to examples below:

example 1: aphicide 1

The embodiment provides an aphicide 1, which comprises the following components in parts by weight:

50 parts of N-methylpyrrolidone, 10 parts of 6-methylcoumarin, 30 parts of turpentine and 10 parts of Tween 85 with the HLB value of 11.

Dissolving 6-methylcoumarin in N-methylpyrrolidone, adding Tween 85 with HLB value of 11 and turpentine, dissolving, and mixing to obtain aphicide 1, and spraying to prevent and treat Aphis gossypii. The aphicide can also control myzus persicae, aphids radishes, cabbage aphids or melon aphids.

Example 2: aphicide 2

The embodiment provides an aphicide 2, which comprises the following components in parts by weight:

50 parts of N-methylpyrrolidone, 5 parts of 6-methylcoumarin, 30 parts of turpentine and 10 parts of Tween 85 with the HLB value of 11.

Dissolving 6-methylcoumarin in N-methylpyrrolidone, adding Tween 85 with HLB value of 11 and turpentine, dissolving, and mixing to obtain aphicide 2, and spraying to prevent and treat Myzus persicae. The aphicide can also control cotton aphid, radish aphid, cabbage aphid or melon aphid.

Example 3: aphicide 3

The embodiment provides an aphicide 3, which comprises the following components in parts by weight:

50 parts of N-methylpyrrolidone, 15 parts of 6-methylcoumarin, 30 parts of turpentine and 5 parts of Tween 85 with the HLB value of 11.

Dissolving 6-methylcoumarin in N-methylpyrrolidone, adding Tween 85 with HLB value of 11 and turpentine, dissolving, and mixing to obtain aphicide 3, and spraying to prevent and treat radish aphid. The aphicide of the embodiment can also control cotton aphids, green peach aphids, cabbage aphids or melon aphids.

The following is a biological activity experiment of the aphicide on cotton aphids.

The aphicide 1 prepared by the formula in example 1 was diluted according to table 1, and an indoor toxicity experiment and a potting control experiment for cotton aphids were performed, the experimental results are shown in tables 2 and 3, and meanwhile, imidacloprid was used as a control group to perform the potting control experiment, the experimental results are shown in table 3, and the control effect graph is shown in fig. 1-3.

TABLE 1 dilution factor comparison Table

TABLE 2 indoor contact killing Activity of aphicide 1 on Aphis gossypii

TABLE 3 control of Cotton aphid potted plant by aphicide 1 (15d)

And (4) analyzing results:

1. in an indoor cotton aphid contact activity experiment, when the concentration of 6-methylcoumarin is 0.03125mg/mL, the mortality rate is 16.07%; when the concentration is 2mg/mL, the death rate is 100%, which indicates that the 6-methylcoumarin has the effect of killing aphids, and has obvious contact killing activity on cotton aphids after being prepared into the aphicide.

2. In the control effect experiment of cotton aphid in indoor potted plant, when the concentration of 6-methylcoumarin is 2mg/mL, the control effect on cotton aphid is up to 76.28 percent after 15 days of pesticide application; when the concentration is 0.125mg/mL, the control effect reaches 30.11% in 15 days, which shows that the 6-methylcoumarin has obvious contact killing activity on cotton aphids after being prepared into the aphicide and can be used for preventing and controlling aphids.

3. After the cotton aphids are treated by the mature commercial pesticide imidacloprid for 15 days, the control effect is 80%, compared with the commercial pesticide imidacloprid, the aphid killing agent containing 6-methylcoumarin has the highest control effect of 76.28%, and has better aphid control effect, which indicates that the 6-methylcoumarin has good pesticide development and application values and is worthy of popularization.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims

The application of 6-methylcoumarin in an aphicide is characterized in that the 6-methylcoumarin is applied in aphidicide.
2. The use of 6-methylcoumarin as an aphicide according to claim 1, wherein the 6-methylcoumarin aphicide is used to kill aphids of the species Aphis citricola.
3. The use of 6-methylcoumarin in aphicides according to claim 2, wherein the aphid of the aphid genus aphis specifically comprises a cotton aphid, a green peach aphid, a radish aphid, a cabbage aphid, or a melon aphid.
The 6-methylcoumarin aphicide is characterized by comprising 6-methylcoumarin, a solvent, an emulsifier and a solubilizer.
5. The 6-methylcoumarin aphicide according to claim 4, wherein the aphicide consists of the following components in parts by weight: 5-15 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 5-15 parts of emulsifier.
6. The 6-methylcoumarin aphicide according to claim 5, wherein the aphicide consists of the following components in parts by weight:

5 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 15 parts of emulsifier;

or 10 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 10 parts of emulsifier;

or 15 parts of 6-methylcoumarin, 50 parts of solvent, 30 parts of solubilizer and 5 parts of emulsifier.
7. The 6-methylcoumarin aphicide according to claim 6, wherein the solvent is N-methylpyrrolidone.
8. The 6-methylcoumarin aphicide according to claim 7, wherein the emulsifier is span, tween.
9. The 6-methylcoumarin aphicide of claim 8, wherein the solubilizing agent is turpentine.
10. An aphicide of 6-methylcoumarin according to any one of claims 4 to 9, characterized in that it is prepared by a process comprising: dissolving 6-methylcoumarin in solvent, adding emulsifier and solubilizer, and mixing.