CN103833458A - Preparation method of efficient organic liquid compound enzyme bacterial fertilizer - Google Patents
Preparation method of efficient organic liquid compound enzyme bacterial fertilizer Download PDFInfo
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- CN103833458A CN103833458A CN201210482198.2A CN201210482198A CN103833458A CN 103833458 A CN103833458 A CN 103833458A CN 201210482198 A CN201210482198 A CN 201210482198A CN 103833458 A CN103833458 A CN 103833458A
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- efficient organic
- organic liquid
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Abstract
A preparation method of an efficient organic liquid compound enzyme bacterial fertilizer is characterized in that the formula is prepared by raw materials of urea phosphate (solution), a biological bacterial agent (fermentation liquid), a trace element combination (solution), a glucose plant amino acid liquid, a humic acid liquid, xanthan gum, sodium ethylene diamine tetracetate, an active ingredient and a plant regulator. Through the preparation, a microbiology theory is utilized, acid-producing beneficial bacteria and organic matters are fermented and then are compounded with the active ingredient promoting plant growth and various trace elements required by plants to prepare the product, so as to improve the soil utilization rate, increase the yield, and improve the crop quality. The formula is reasonable, the production cost is low, and the cost can be controlled in 20-50 yuan calculated according to 25 ml per barrel; and the use effect is good, the yield can be increased by 5-10% through applying with 2 barrels per mu, and moreover the quality of agricultural products can be improved. Therefore, the product is worth of popularization.
Description
Technical field
The present invention relates to microorganism and chemical field, particularly a kind of preparation method of efficient organic liquid prozyme bacterial manure.
Background technology
Due to life-time service chemical fertilizer, cause soil compaction, a large amount of nutrients are coated, make nitrogen, phosphorus and various trace elements in soil can not discharge and be absorbed by plants.
Summary of the invention
The present invention proposes a kind of preparation method of efficient organic liquid prozyme bacterial manure, it is characterized in that: this formula comprises, with raw material Ureaphil (solution), bacteria agent (fermented liquid), micro-summation (solution), grape sugar refinery aminoacids solution, humic acid solution, xanthan gum, sodium ethylene diamine tetracetate, activeconstituents, plant modifying agent is formulated.
By the preparation of this invention, utilize microbiology theory, producing after sour probiotics and organic matter fermentation again and the activeconstituents of Promoting plant growth and the required compound product that is mixed with of various trace elements of plant, be intended to improve Soil Utilization rate, increase output, improve crop quality.The present invention fills a prescription rationally, and production cost is low, calculates by every barrel of 25ml, and cost can be controlled between 20-50 unit; Result of use is good, uses 2 barrels for every mu and can increase production 5-10%, and can improve agricultural product quality.Therefore this product is worthy to be popularized.
brief description of the drawings
Figure is formula of the present invention.
embodiment
1, the fermentation of bacteria agent
(1) screening of phytase-producing strain (aspergillus niger) and liquid state fermentation are cultivated
Phytase-producing strain primary dcreening operation: this surrounding region of city soybean root pedotheque is packed in the triangular flask with sterilized water, get supernatant liquid and also coat in screening culture medium after dilution after vibrating, leaving standstill.Constant temperature culture 3～4 days at 37 DEG C.
Phytase-producing strain sieves again: single bacterium colony of picking transparent circle maximum is in multiple sieve substratum.Cultivate 2～3 days.
Liquid state fermentation is cultivated: the bacterial strain after isolation identification produces in enzymic fermentation substratum and cultivates at liquid.Inoculum size is 10%, and leavening temperature is 30 DEG C, and fermentation time is 84h.
Wherein: primary dcreening operation substratum is glucose 30.0g, NH
4nO
35.0g, KCl
0.5g，MgSO
4·7H
2O?0.5g，MnSO
4·7H
2O?0.05g，FeSO
4·?7H
2O
0.05 g, agar 20.0 g, phytic acid ca 1.0g, water 1L, pH 5. 5～6.5.
Sieve again substratum glucose 30.0g, peptone 3.0g, (NH
4)
2sO
42.0 g, KCl 0.5g, MgSO
47H
2o 0.3g, MnSO
47H
2o 0.05g, FeSO
47H
2o 0.05g, water 1 000mL, pH 5. 4～5. 6.
It is Zulkovsky starch 4% that liquid produces enzymic fermentation substratum, NH
4nO
30.08%, KCl 0.05%, MgSO
47H
2o 0.05%, FeSO
47H
2o 0.003%, MnSO
47H
2o 0. 003%, substratum Initial pH 6.0.
(2) screening of phosphate-solubilizing bacteria and liquid state fermentation are cultivated
Phosphate-solubilizing bacteria bacterial strain primary dcreening operation: this city Suburb Soil sample is loaded on in the triangular flask of sterilized water, gets supernatant liquid and also coat on organophosphorus culture medium flat plate after dilution after vibrating, leaving standstill.37 DEG C of constant temperature culture 2～3 days.
Phosphate-solubilizing bacteria bacterial strain sieves again: the bacterial strain that has solution organophosphorus ability after separation and purification is planted in liquid culture and concentrated, and 37 DEG C, 150r/min, cultivates 48h.
Fermentation culture: the strongest single strain of phosphorus decomposing ability after multiple sieve is cultivated in fermention medium.Ventilating, under 37 DEG C of conditions, cultivate 3 days.
Wherein: organophosphorus substratum glucose 10g, (NH4)
2sO
40.5g, MgSO
47H
2o 0.3 g, NaCl 0.3 g, KCl 0.3g, FeSO
47H
2o 0.036g, MnSO
47H
2o 0.03g, CaCO
35 g, agar 20 g, distilled water 1000 mL, bacterium yolk 10%(volume fraction).
Liquid nutrient medium is that organophosphorus substratum does not add agar.
Fermention medium: 1% Semen Maydis powder, 2%NH
4cl, CaCO
30.1%, MnSO
40.1%, K
2hPO
40.1%, pH value is 7.
(3) fermented liquid mixes and microbial inoculum moulding
By phytase-producing strain fermented liquid with phosphate-solubilizing bacteria fermented liquid by volume for 1:1 mixes.
chemistry organic active composition and trace element are produced
(1) get the distilled water of 70～80 DEG C, by after being metered into ferrous sulfate, copper sulfate, manganous sulfate, zinc sulfate, magnesium sulfate and rare earth and dissolving, add while hot sequestrant sodium ethylene diamine tetracetate, fully stir and make its complete chelating.
(2) distilled water of 70～80 DEG C is injected in mixer, by being metered into 5 nitroguaiacol sodium, sodium onitrophenol, Sodium p-nitrophenoxide, stir it is dissolved completely.The solution of preparing add (1) under fully stirring in, fills up required distilled water, then to add boric acid, potassium primary phosphate to be adjusted to pH value be 7～8 buffering system.
the mixing of bacteria agent and additive
By a certain percentage bacteria agent mixed solution is fully mixed with organic active composition mixed solution, then add appropriate ammonium molybdate, Sodium Selenite, urea, sodium laurylsulfonate, after fully stirring, obtain product.
Organic fermented product, humic acids, probiotic bacterium etc. are added starch slurry fermented liquid and aminoacids solution by the present invention, makes mixing liquid.It is soluble in water, and farm crop absorb rapidly, easy to use, can spray, rush and execute.For farmland, can improve soil, discharge nutrient, again can storing water and preserving fertility; For food crop, vegetables, melon and fruit, flowers etc., can promote plant metabolism, opposing pathogenic bacteria, produces benefit materials control farm crop various diseases, promotes growing of plant, increases social benefit.
Claims (1)
1. the preparation method of an efficient organic liquid prozyme bacterial manure, it is characterized in that: this formula comprises, with raw material Ureaphil (solution), bacteria agent (fermented liquid), micro-summation (solution), grape sugar refinery aminoacids solution, humic acid solution, xanthan gum, sodium ethylene diamine tetracetate, activeconstituents, plant modifying agent is formulated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210482198.2A CN103833458A (en) | 2012-11-25 | 2012-11-25 | Preparation method of efficient organic liquid compound enzyme bacterial fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210482198.2A CN103833458A (en) | 2012-11-25 | 2012-11-25 | Preparation method of efficient organic liquid compound enzyme bacterial fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103833458A true CN103833458A (en) | 2014-06-04 |
Family
ID=50797402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210482198.2A Pending CN103833458A (en) | 2012-11-25 | 2012-11-25 | Preparation method of efficient organic liquid compound enzyme bacterial fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103833458A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748259A (en) * | 2017-01-17 | 2017-05-31 | 四川鹤岛农业科技有限公司 | A kind of retain water and nutrients composite microbic bacterial fertilizer and application |
| CN109503234A (en) * | 2018-12-06 | 2019-03-22 | 中国热带农业科学院农产品加工研究所 | A kind of ammino acid liquid fertilizer and preparation method thereof comprehensively utilizing discarded marine product preparation |
| CN109618997A (en) * | 2018-12-25 | 2019-04-16 | 浙江海洋大学 | The ecological purification method of oyster culture |
-
2012
- 2012-11-25 CN CN201210482198.2A patent/CN103833458A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106748259A (en) * | 2017-01-17 | 2017-05-31 | 四川鹤岛农业科技有限公司 | A kind of retain water and nutrients composite microbic bacterial fertilizer and application |
| CN109503234A (en) * | 2018-12-06 | 2019-03-22 | 中国热带农业科学院农产品加工研究所 | A kind of ammino acid liquid fertilizer and preparation method thereof comprehensively utilizing discarded marine product preparation |
| CN109618997A (en) * | 2018-12-25 | 2019-04-16 | 浙江海洋大学 | The ecological purification method of oyster culture |
| CN109618997B (en) * | 2018-12-25 | 2021-05-11 | 浙江海洋大学 | Ecological purification method for oyster cultivation |
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140604 |