CN103828704A - Method for cultivating fine breed of hybrid rice by utilizing wild rice - Google Patents
Method for cultivating fine breed of hybrid rice by utilizing wild rice Download PDFInfo
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- CN103828704A CN103828704A CN201210480523.1A CN201210480523A CN103828704A CN 103828704 A CN103828704 A CN 103828704A CN 201210480523 A CN201210480523 A CN 201210480523A CN 103828704 A CN103828704 A CN 103828704A
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Abstract
The invention relates to a method for cultivating a fine breed of hybrid rice by utilizing wild rice. The method includes the specific steps of: first, using a castrated IR58025B as a female parent and hybridizing the female parent with a wild rice to obtain an F1; then using a castrated IR58025B as a female parent and hybridizing the female parent with F1 to obtain an F2; then using the same method for hybrid to obtain an F3; then conducting germination on the F3 grains, using solar energy to carry out focus radiation on the bottoms of the grain sprouts, and breeding a radiation generation 1 seed-grain; selecting dominant plants from the radiation generation 1, and conducting continuous breeding to obtain a radiation generation 4 crossbred wild B, and selecting 35 plants; and carrying out 35 groups of paring hybrid by using IR58025A and the crossbred wild B, selecting close pairing in an A*B growth period, and mainly selecting B line plants with large column, strong vigor and early flowering; then respectively sowing the 35 groups of paring hybrid seeds, planting by germ lines, and conducting microscopic examination to find out 3 groups of aborted sterile germ lines; and finally carrying out separate nursery protection and nursery selection at the same time, and separately collecting and planting the seeds by groups, so as to finally obtain the strong dominant hybridized combination.
Description
Technical field
The present invention relates to a kind of rice breeding new technical field, be specifically related to a kind of method of utilizing wild rice to cultivate hybrid rice breeding.
Technical background
Because wild rice is for a long time in wild state, stand the natural selection of various poor environments and disaster, the excellent adversity gene that they are contained is very abundant.Major embodiment is being contained the excellent genes such as a large amount of Resistants, drought resisting, cold-resistant, high yield, high-quality and high light efficiency in: wild rice, comprising genetic resourceses such as disease and insect resistance (as bacterial leaf-blight, rice blast, banded sclerotial blight, brown planthopper, white-backed planthopper, leafhopper, rice gall midge, yellow dwarf, general short disease), resistance (cold-resistant, salt tolerant, drought-enduring, waterlogging) and other merits (as cytoplasmic male sterility, the resistance to aging of functional leaf, reviviscence, high-quality).Thereby wild rice has unique effect in breeding, it is the important materials of rice breeding.So, wild rice is the valuable genetic resources of rice breeding, last century the seventies, " father of Chinese hybrid rice " Academician YUAN Long-ping be exactly utilize suburb, Sanya, Hainan Province find common wild-rice be breach, started the new situation of series of three-series hybrid rice breeding.
Because wild rice has the advantage of different resistances on gene, therefore hybridize the new varieties of cultivating by wild rice with cultivated rice, just there is wild rice well developed root system, strong, the high Resistant of tillering ability and the large advantage of Ye Se dark green four.The assorted excellent paddy rice of promoting at most of China at present, is all to use wild rice and cultivated rice cross breeding.
Because wild paddy rice itself is there is no direct value, and mainly with sprawl growth, granule, small ear, paddy has long awns, and paddy maturation is inconsistent, and ripe seed drops once shaking, and the defect such as yield poorly and cannot gather in.Its advantage is only embodied in the feature with special resistance.So it is long that the Period Process of new rice variety is cultivated in the hybridization of the resistance of traditional wild rice and cultivated rice, the advantage plant stability of cultivating is not good, then affects rice yield and quality.
Summary of the invention
Object of the present invention is just to provide a kind of brand-new method of utilizing wild rice fast and stable to cultivate hybrid rice breeding.Specifically comprise: first make female parent with IR58025B emasculation and hybridize to obtain F with wild rice
1, then make female parent and F with IR58025B emasculation
1hybridize to obtain F
2, then hybridize to obtain F with same method
3, then F
3grain carries out vernalization, utilize solar energy focus radiation rice sprout base portion and select spoke 1 generation seed-grain, then from spoke 1 generation plant, select dominant strain continuous seed selection and obtain wild assorted B of 4 generations of spoke, therefrom select 35 individual plants, do to hand over 35 groups in pairs with IR58025A and wild assorted B again, A × B selects to approach pairing breeding time, and main separation post is large, the B strain of energetic, Hua Shizao; Then the paired friendship seed of 35 groups being sowed respectively, point is plantation, and microscopy occurs that 3 groups is that allusion quotation loses for complete sterility, selects Bao Pu and selects for rear point and join garden and carry out simultaneously, and seed is by group, point contracture kind, finally the hybrid combination of strong advantage.
The present invention is achieved through the following technical solutions:
1, utilize wild rice to cultivate a method for hybrid rice breeding, in technical scheme, comprise the steps:
(1) make female parent with IR58025B emasculation and obtain the wild assorted upright strain F of purple stalk with wild rice hybridization
1;
(2) make maternal and wild assorted upright strain F with IR58025B emasculation
1pollination obtains purple stalk strain F
2, and cultivate to obtain grain;
(3) make maternal and wild assorted upright strain F with IR58025B emasculation
2pollination obtains purple stalk strain F
3, and cultivate to obtain grain;
(4) in container, load onto sandy soil, then by assorted open country F
3grain is evenly broadcast on sandy soil, and vernalization according to a conventional method, in the time that rice sprout grows to 0.5～1.5cm length, utilizes solar energy focus to carry out radiation to bastem portion;
(5) will cultivate spoke 1 generation seed-grain through the kind bud of solar energy focus radiation;
(6) by spoke 1 generation seed-grain sowing plantation 0.5～10,000 strain, therefrom select 4 dominant strain, it is receipts that seed divides, and obtains spoke 2 generation seed-grain;
(7) 4 spoke 2 generation dominant strain are planted respectively to 800～1200 strains, then select 3 dominant strain from 4 strains, then cultivate to obtain spoke 3 generation seed-grain;
(8) by various 200～300 strains of 3 spoke 3 generation dominant strain, respectively select 1 strain from 3 strains, single sowing is wild assorted B;
(9) planting respectively IR58025A and the wild B of mixing does to hand over to select in pairs to guarantee;
(10) do to hand over 35 groups in pairs with IR58025A and wild assorted B, A × B selects to approach combo breeding time, and main separation column cap is large, energetic, Hua Shizao as B strain;
(11) the paired friendship seed of 30～40 groups is sowed respectively, dividing is plantation, and microscopy occurs that 3 groups is that allusion quotation loses complete sterility germline;
(12) divide to select Bao Pu and select and join garden and carry out simultaneously, seed is by group, point contracture kind, finally the hybrid combination of strong advantage.
2, utilize wild rice to cultivate a method for hybrid rice breeding, described in technical scheme, utilize solar energy focus to the assorted F in open country
3rice sprout base portion carries out radiation, and concrete grammar is as follows:
(1) select 8cm magnifying glass to gather the base portion of focus radiation bud of sunshine;
(2) first step: with the large focus radiation of broad bean 30 seconds;
(3) second step: with the large focus radiation of watermelon seeds 2 minutes;
(4) the 3rd steps: be radiated rice sprout base portion with a large focus of soya bean and occur that micro-phenomenon of smoldering stops, and drip the moistening bud paddy of mineral water.
3, utilize wild rice to cultivate a method for hybrid rice breeding, described in technical scheme, utilize solar energy focus to the assorted F in open country
3the Best Times that rice sprout base portion carries out radiation is the morning 10～11 point of fine day.
The present invention both had the following advantages:
1, rice breeding method measure uniqueness proposed by the invention, easily enforcement.
2, by implementing the present invention, adopt radiation method, planting percent is low, aberration rate is higher, mostly appears as that plant is downgraded, tassel diminishes, solid raising, degeneration-resistant enhancing, also occur less sterile strain and half sterile strain, radiation produces variation greatly and is stable fast, provides stable, good important channel for cultivating good rice paddy seed.
3, by implementing the present invention, cultivate and obtain better advantage strain.
4, by implementing the present invention, obtain the new rice variety of fast and stable.
5, by implementing the present invention, cultivate high-quality female parent, for hybridization superior rice is obtained important breakthrough.
Accompanying drawing explanation
It shown in Fig. 1, is exactly the paddy rice breeding hybridization cultivating process described in the embodiment of the present invention.
Embodiment
Below in conjunction with embodiment, method of the present invention is further illustrated.
Utilize wild rice to cultivate hybrid rice breeding, embodiment is as follows:
1, make female parent with IR58025B emasculation and obtain the wild assorted upright strain F of purple stalk with wild rice hybridization
1.
2, make maternal and wild assorted upright strain F with IR58025B emasculation
1pollination obtains purple stalk strain F
2, and cultivate to obtain grain.
3, make maternal and wild assorted upright strain F with IR58025B emasculation
2pollination obtains purple stalk strain F
3, and cultivate to obtain grain.
4, in container, load onto sandy soil, then by assorted open country F
3grain is evenly broadcast on sandy soil, and vernalization according to a conventional method, in the time that rice sprout grows to 0.5～1.5cm length, utilizes solar energy focus to carry out radiation to bastem portion.
5, will cultivate spoke 1 generation seed-grain through the kind bud of solar energy focus radiation.
(1) select 8cm magnifying glass to gather the base portion of focus radiation bud of sunshine;
(2) utilize the morning 10～11 period for fine day to start to carry out;
(3) first step: with the large focus radiation of broad bean 30 seconds;
(4) second step: with the large focus radiation of watermelon seeds 2 minutes;
(5) the 3rd steps: be radiated rice sprout base portion with a large focus of soya bean and occur that micro-phenomenon of smoldering stops, and drip the moistening bud paddy of mineral water.
6, by spoke 1 generation seed-grain sowing plantation 0.5～10,000 strain, therefrom select 4 dominant strain, it is receipts that seed divides, and obtains spoke 2 generation seed-grain.
7,4 spoke 2 generation dominant strain are planted respectively to 800～1200 strains, then select 3 dominant strain from 4 strains, then cultivate to obtain spoke 3 generation seed-grain.
8, by various 200～300 strains of 3 spoke 3 generation dominant strain, respectively select 1 strain from 3 strains, single sowing is wild assorted B.
9, planting respectively IR58025A and the wild B of mixing does to hand over to select in pairs to guarantee.
10, do to hand over 35 groups in pairs with IR58025A and wild assorted B, A × B selects to approach combo breeding time, and main separation column cap is large, the B strain of energetic, Hua Shizao.
11,30～40 groups are obtained and hand in pairs seed to sow respectively, dividing is plantation, and microscopy occurs that 3 groups is that allusion quotation loses complete sterility germline.
12, divide to select Bao Pu and select and join garden and carry out simultaneously, seed is by group, point contracture kind, finally the hybrid combination of strong advantage.
Embodiment 1:
1, make female parent with IR58025B emasculation and obtain the wild assorted upright strain F of purple stalk with the hybridization of Java wild rice
1, plant height 130cm, has long awns.
2, make the maternal and wild assorted upright strain F in Java with IR58025B emasculation
1pollination obtains purple stalk strain F
2, plant height 110cm, has awns, and cultivates to obtain elongated grain.
3, make the maternal and wild assorted upright strain F in Java with IR58025B emasculation
2pollination obtains purple stalk strain F
3, plant height 105cm, and cultivate to obtain elongated grain.
4, in container, load onto sandy soil, then by assorted open country F
3grain is evenly broadcast on sandy soil, and vernalization according to a conventional method, when rice sprout grows to 1 grain～1 paddy when long, utilizes solar energy focus to carry out radiation to its base portion.
5, will cultivate spoke 1 generation seed-grain through the kind bud of solar energy focus radiation.
(1) select 8cm magnifying glass to gather the base portion of focus radiation bud of sunshine;
(2) utilize the morning 10～11 period for fine day to start to carry out;
(3) first step: with the large focus radiation of broad bean 30 seconds;
(4) second step: with the large focus radiation of watermelon seeds 2 minutes;
(5) the 3rd steps: be radiated radiant face with a large focus of soya bean and occur that micro-phenomenon of smoldering stops, and with the moistening bud paddy of mineral spring water droplet.In 1 generation of spoke, survives 13 strains, mixes receipts by cultivating seed.
6, by spoke 1 generation seed-grain sowing plantation 10,000 strains, therefrom select 4 dominant strain, it is receipts that seed divides, and obtains spoke 2 generation seed-grain.
7,4 spoke 2 generation dominant strain are planted respectively to 800 strains, then select 3 dominant strain from 4 strains, then cultivate to obtain spoke 3 generation seed-grain.
8, by various 200 strains of 3 spoke 3 generation dominant strain, 3 strain stable and consistent, respectively select 1 strain from 3 strains, single sowing is wild assorted B.
9, planting respectively the wild assorted B in IR58025A and Java does to hand over to select in pairs to guarantee.
10, do to hand over 35 groups in pairs with the wild assorted B in IR58025B and Java, A × B selects to approach combo breeding time, and main separation post is large, the B strain of work early when energetic, flower.
11,35 groups are obtained and hand in pairs seed to sow respectively, dividing is plantation, and microscopy occurs that 3 groups is that allusion quotation loses complete sterility germline.
12, divide to select Bao Pu (A × B) and select and join garden (A × R) and carry out simultaneously, seed is by group, point contracture kind, finally the hybrid combination of strong advantage.
Claims (3)
1. utilize wild rice to cultivate a method for hybrid rice breeding, its feature comprises the steps:
(1) make female parent with IR58025B emasculation and obtain the wild assorted upright strain F of purple stalk with wild rice hybridization
1;
(2) make maternal and wild assorted upright strain F with IR58025B emasculation
1pollination obtains purple stalk strain F
2, and cultivate to obtain grain;
(3) make maternal and wild assorted upright strain F with IR58025B emasculation
2pollination obtains purple stalk strain F
3, and cultivate to obtain grain;
(4) in container, load onto sandy soil, then by assorted open country F
3grain is evenly broadcast on sandy soil, and vernalization according to a conventional method, in the time that rice sprout grows to 0.5～1.5cm length, utilizes solar energy focus to carry out radiation to bastem portion;
(5) will cultivate spoke 1 generation seed-grain through the kind bud of solar energy focus radiation;
(6) by spoke 1 generation seed-grain sowing plantation 0.5～10,000 strain, therefrom select 4 dominant strain, it is receipts that seed divides, and obtains spoke 2 generation seed-grain;
(7) 4 spoke 2 generation dominant strain are planted respectively to 800～1200 strains, then select 3 dominant strain from 4 strains, then cultivate to obtain spoke 3 generation seed-grain;
(8) by various 200～300 strains of 3 spoke 3 generation dominant strain, respectively select 1 strain from 3 strains, single sowing is wild assorted B;
(9) planting respectively I R58025A and the wild B of mixing does to hand over to select in pairs to guarantee;
(1O) do to hand over 35 groups in pairs with IR58025A and wild assorted B, A × B selects to approach combo breeding time, and main separation column cap is large, energetic, Hua Shizao as B strain;
(11) the paired friendship seed of 30～40 groups is sowed respectively, dividing is plantation, and microscopy occurs that 3 groups is that allusion quotation loses complete sterility germline;
(12) divide to select Bao Pu and select and join garden and carry out simultaneously, seed is by group, point contracture kind, finally the hybrid combination of strong advantage.
2. utilize wild rice to cultivate the method for hybrid rice breeding according to a kind of described in claim 1, it is characterized in that step (4) described utilize solar energy focus to the assorted F in open country
3rice sprout base portion carries out radiation, and method is as follows:
(1) select 8cm magnifying glass to gather the base portion of focus radiation bud of sunshine;
(2) first step: with the large focus radiation of broad bean 30 seconds;
(3) second step: with the large focus radiation of watermelon seeds 2 minutes;
(4) the 3rd steps: be radiated rice sprout base portion with a large focus of soya bean and occur that micro-phenomenon of smoldering stops, and drip the moistening bud paddy of mineral water.
3. utilize wild rice to cultivate the method for hybrid rice breeding according to a kind of described in claim 1, it is characterized in that step (4) described utilize solar energy focus to the assorted F in open country
3the Best Times that rice sprout base portion carries out radiation is the morning 10～11 point of fine day.
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| CN201210480523.1A CN103828704A (en) | 2012-11-23 | 2012-11-23 | Method for cultivating fine breed of hybrid rice by utilizing wild rice |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105594589A (en) * | 2016-03-10 | 2016-05-25 | 周重学 | Rapid breeding method for salt and alkali rice |
| CN105766628A (en) * | 2016-03-10 | 2016-07-20 | 周安岳 | Method for maintaining rapid advantages of variant lines stable with solar energy focal radiation on sprout grains |
| CN106852277A (en) * | 2016-12-28 | 2017-06-16 | 陵水重学辐射育种研究所 | A kind of novel method for cultivating of saline and alkaline rice sterile line |
| CN106900542A (en) * | 2017-03-31 | 2017-06-30 | 陵水重学辐射育种研究所 | A kind of method that utilization wild rice cultivates saline and alkaline rice |
-
2012
- 2012-11-23 CN CN201210480523.1A patent/CN103828704A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105594589A (en) * | 2016-03-10 | 2016-05-25 | 周重学 | Rapid breeding method for salt and alkali rice |
| CN105766628A (en) * | 2016-03-10 | 2016-07-20 | 周安岳 | Method for maintaining rapid advantages of variant lines stable with solar energy focal radiation on sprout grains |
| CN106852277A (en) * | 2016-12-28 | 2017-06-16 | 陵水重学辐射育种研究所 | A kind of novel method for cultivating of saline and alkaline rice sterile line |
| CN106900542A (en) * | 2017-03-31 | 2017-06-30 | 陵水重学辐射育种研究所 | A kind of method that utilization wild rice cultivates saline and alkaline rice |
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Application publication date: 20140604 |