CN105766628A - Method for maintaining rapid advantages of variant lines stable with solar energy focal radiation on sprout grains - Google Patents
Method for maintaining rapid advantages of variant lines stable with solar energy focal radiation on sprout grains Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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Abstract
The invention relates to a method for maintaining rapid advantages of variant lines stable with solar energy focal radiation on sprout grains. The method specially comprises the following steps: selecting grain seeds; performing moistening and sprouting; placing the seeds in a certain shape; performing dibble seeding in a pyramid shape; performing radiation on the seeds in three steps; performing optimization on plants; performing backcrossing; obtaining advantage polymerization. The method disclosed by the invention is implemented, the technology is simple, the operation is easy, and only simple appliances including magnifying glass and the like are needed; but the variant probability is high, and the rapid advantages of the variant lines are maintained stable. The method disclosed by the invention is implemented, the first generation varies, and the variation rate reaches 15% or above. Sterile plants appearing in the method provide a new way for cultivating novel female parents. Besides, after backcrossing, new varieties are bred, and have the advantages of lodging resistance, high temperature resistance, broad adaptability, steady yield, and excellent color, fragrance and taste of rice. The yield of cultivated saline-alkaline resistant two-line sterile lines and the yield of early-maturing conventional rice, mid-maturing conventional rice and late-maturing conventional rice are increased by 2 times or above than that of original seawater rice. The bred three-line sterile lines have the characteristics and the functions that steamed red rice, steamed black rice and steamed brown rice are fragrant and soft, and steamed rice does not become hard after being put for a night.
Description
Technical field
The present invention relates to a kind of new rice variety and cultivate new technical field, be specifically related to a kind of utilize solar energy focus radiation bud paddy can keep the method that the variation quick advantage of strain is stable.
Background technology
Being studied and obtain some genes relevant to plant growing, growth and other character by mutant is one of important Molecular and Genetic Study means, is currently engaged in scholar's extensive use of basic research.Utilize induced-mutation technique to carry out breeding rice breeding, be that one carries out resource innovation and effective method.
Learn from pertinent literature.At present, the induced-mutation technique being applied to breeding rice breeding mainly has the ways and means such as pressure induced, radioinduction, Ion Implantation in Rice mutation, space mutagenesis.
Seed is carried out 75-78Mpa pressurized treatments, have studied the buckling effect that paddy growth is produced by high pressure. result shows: relatively low through the Germination Percentage of Rice of HIGH PRESSURE TREATMENT, sprout time retardation, although growth of seedling speed after sprouting, but the inhibitory action of its growth is still continued by pressure, just fade away until rice tillering contains phase this impact；Additionally, it is observed that high pressure energy causes Rice Physiological to produce change, as caused chlorophyll content of rice increase etc.: high pressure also can cause Oryza sativa L. to produce hereditary variation, but the probability that variation occurs is only small and different because of the genetic background of kind, do not find that variation has directivity simultaneously.Field planting result shows, high pressure is inconspicuous on the impact of the Other Main Agronomic Characters such as plant height, spike number of Rice Population, fringe weight, grain number, mass of 1000 kernel, florescence etc..
Radioinduction has inducing plant genetic gene mutation, chromosome disorder, promotes recombination of genes, improves the features such as recombination fraction, is the effective means of Crop Improvement.It addition, the improvement of radioinduction technology, to improving breeding efficiency and level, the innovation of genetic resources, the incubation of improved seeds has important theory and practice meaning.Rice paddy seed is by physical mutagenesis such as radiation treatment, the suppression of its generation or damage effect are strong, the ionizing radiation that physical mutagenesis is representative with R ray and X ray, it has been found that it is stronger that seed is obtained penetration power by it, easily it is colored body group to absorb, chromosome structure is had very big destructiveness.Above-mentioned radiation treatment can also the growth of the sprouting of strong inhibition crop seed and root, bud.
Chinese Academy of Sciences Hefei plasma physics institute Yu Zengliang assistant researcher cooperates with Paddy Rice Inst., Anhui Agriculture Science Academy, has started the new way of Ion Implantation in Rice mutagenic breeding.Ion implantation mutation breeding breaches the concept of other radioactive breeding.Its starting point is to implant ions in certain part of crop seed, causes this part genetic gene mutation.Signal preliminary study in the past few years in Ion Implanted Rice Seed embryo portion shows, Ion Implantation in Rice not only planting percent high (90%) also can obtain high mutation rate (8.4%) and wide mutational spectrum.
Oryza sativa L. space breeding technology (RiceSpaceflightBreeding) is that LIPIDS OF DRY RICE EMBRYO seed is equipped on recoverable spacecraft (satellite), it is made to produce variation through space mutagenesis effect, the breeding method of then evolve new breeds in the ground useful variation of selection matter, new varieties.Since Guangxi Agriculture Univ. in 1988 cooperates with Institute of Genetics, Academia Sinica, since utilizing Seeds of First Post-flight photosensitive type rice paddy seed " bag selects No. 2 " first and therefrom bringing out temperature sensitive type high yield strain, Oryza sativa L. just becomes the focal selection object of space breeding, has been bred as multiple rice varieties popularization and application aborning.But Space Mutation Breeding of Rice has focused largely on the excellent mutant of field selection-breeding to obtain the aspects such as new breeding material at present, and the fundamental research of Space Mutation Breeding is not deep enough, it is particularly that the character variation of kind space carrying offspring is less with Genetic conditions research to the restorer of different genes source and different genetic background and maintenance.Meanwhile, utilizing space breeding, breeding condition is limited to.
Induced-mutation technique described above, the not only technology specialty implemented and complexity, but also need special facilities and equipment.It addition, the variability probability that most method realizes is smaller, among such as 10,000 strains, only have a few strain variants.Original report radiaction mutation, the second filial generation produces variation, and aberration rate is ten thousand/left and right.So, if to realize the strain that makes a variation in a large number, then large-area soil is needed to implement, in addition it is also necessary to much manually managing, production cost is greatly improved.
Summary of the invention
The purpose of the present invention is exactly overcome the deficiency of above-mentioned technology and provide brand-new and a kind of utilize solar energy focus radiation bud paddy can keep the method that the variation quick advantage of strain is stable.Specifically include: select seed-grain → moistening accelerating germination → seed pendulum shape → program request to become pyramid → seed to divide three steps to carry out radiating → preferred plant → carry out → acquisition advantage the polymerization that backcrosses.By sunlight, Jiao radiates, and technique is simply, easily operate, it is only necessary to the simple apparatus such as magnifier, but the probability of variation is high, and keeps the variation quick advantage of strain stable.
The present invention is achieved through the following technical solutions:
1, a kind of utilize solar energy focus radiation bud paddy can keep the variation stable method of the quick advantage of strain, technical scheme comprise the following steps:
(1) select seed-grain: screen full millet 100 seed, keep moistening accelerating germination；
(2) seed pendulum shape: the round basin of what a 25～30 cm diameter standby, loads onto moistening thin sand and soil, the seed that will germinate, and every 10 is one group, and program request becomes pyramid, eight pyramids form a basin, it is simple to the record of different time radiation；
(3) seed radiation: when bud paddy grows to 1～5 centimetre with magnifier focus on big sun can focus radiation bastem portion, radiation point three steps carry out: just the light focus big with Semen Viciae fabae is irradiated 30 seconds；Second step is reduced to a Semen Citrulli large radiation 30 seconds heavily to process focus；3rd step is violent process, and focus is reduced to a Semen Glycines size radiation, occurs that namely micro-emitting vaporous stop；Instil after completing two mineral waters every time, with moistening bud paddy；
(4) preferred plant: radiation shoot survival percent is generally between 25～35%, treats that young plant grows to 5 leaves and can transplant to land for growing field crops, examines the situation recording variation subsequently, and optimum selecting has the plant of value；
(5) processing scheme of variant and enforcement: find sterile strain, then plant division is accredited as two and is or three genes being, and carries out its value analysis；The fertile plant of different qualities, backcrosses, it is thus achieved that advantage is polymerized.
2, a kind of solar energy focus radiation bud paddy is utilized can to keep the variation stable method of the quick advantage of strain, technical scheme is in that: seed radiates at Qiu Mo or severe winter, it is heat to 35 degree with a steamer water, food steamer upper berth warp layer cloth, bud paddy swings on gauze, carry out radiation treatment, after the radiation of bud paddy, move into winter numerous base in greenhouse or Hainan.
The present invention both had the following advantages:
1, bud paddy is novel, method and measure is unique in the proposed by the invention solar energy focus radiation that utilizes, and technique is simple, it is easy to implement.
2, by implementing the present invention, technique is simply, easily operate, it is only necessary to the simple apparatus such as magnifier.But the probability of variation is high, and keep the variation quick advantage of strain stable.
3, by implementing the present invention, the first generation produces variation, and aberration rate reaches more than 1 15.The sterile strain occurred, is the new way cultivating novel female parent.
4, by implementing the present invention, after backcrossing, select new varieties, novel sterile line, there is the advantage of anti-fall, high temperature resistant, wide adaptability, stable yields, all U.S.s of rice color, smell and taste, and select and absorb the kind that zinc selenium function is strong.Cultivated the two-line sterile line of Salt And Alkali Tolerance early, in, ripe late conventional Rice；Ratio former sea increasing production of rice more than 2 times, rice matter is also improved, and stem stalk is thicker strong, well developed root system, plant height about 1 meter, resistance to extensive, adapt to live.Selecting CMS line, the novel compositions allotted has: fragrant soft red rice, fragrant soft fructus zizaniae caduciflorae, steamed brown rice perfume (or spice) are soft, the characteristic functions such as overnight rice is not hardening.
5, the present invention is implemented, it is also possible to be applied to Semen Maydis variation.Semen Maydis aberration rate is higher than Oryza sativa L., and the method for radiation is similar to Oryza sativa L..
Detailed description of the invention
Below in conjunction with embodiment, the method for the present invention is further illustrated.
1, a kind of utilize solar energy focus radiation bud paddy can keep the variation stable method of the quick advantage of strain, technical scheme comprise the following steps:
(1) select seed-grain: screen full millet 100 seed, keep moistening accelerating germination；
(2) seed pendulum shape: the round basin of what a 25～30 cm diameter standby, loads onto moistening thin sand and soil, the seed that will germinate, and every 10 is one group, and program request becomes pyramid, eight pyramids form a basin, it is simple to the record of different time radiation；
(3) seed radiation: when bud paddy grows to 1～5 centimetre with magnifier focus on big sun can focus radiation bastem portion, radiation point three steps carry out: just the light focus big with Semen Viciae fabae is irradiated 30 seconds；Second step is reduced to a Semen Citrulli large radiation 30 seconds heavily to process focus；3rd step is violent process, and focus is reduced to a Semen Glycines size radiation, occurs that namely micro-emitting vaporous stop；Instil after completing two mineral waters every time, with moistening bud paddy；
(4) preferred plant: radiation shoot survival percent is generally between 25～35%, treats that young plant grows to 5 leaves and can transplant to land for growing field crops, examines the situation recording variation subsequently, and optimum selecting has the plant of value；
(5) processing scheme of variant and enforcement: find sterile strain, then plant division is accredited as two and is or three genes being, and carries out its value analysis；The fertile plant of different qualities, backcrosses, it is thus achieved that advantage is polymerized.
2, a kind of solar energy focus radiation bud paddy is utilized can to keep the variation stable method of the quick advantage of strain, technical scheme is in that: seed radiates at Qiu Mo or severe winter, it is heat to 35 degree with a steamer water, food steamer upper berth warp layer cloth, bud paddy swings on gauze, carry out radiation treatment, after the radiation of bud paddy, move into winter numerous base in greenhouse or Hainan.
Embodiment 1:
1, seed-grain is selected: screen special excellent millet 100 seed of full Bath Ma Di meter matter, keep moistening accelerating germination.
2, seed pendulum shape: the round basin of what a 25～30 cm diameter standby, loads onto moistening thin sand and soil, the seed that will germinate, and every 10 is one group, and program request becomes pyramid, eight pyramids form a basin, it is simple to the record of different time radiation.
3, seed radiation: when bud paddy grows to 1～5 centimetre with magnifier focus on big sun can focus radiation bastem portion, radiation point three steps carry out: just the light focus big with Semen Viciae fabae is irradiated 30 seconds；Second step is reduced to a Semen Citrulli large radiation 30 seconds heavily to process focus；3rd step is violent process, and focus is reduced to a Semen Glycines size radiation, occurs that namely micro-emitting vaporous stop；Instil after completing two mineral waters every time, with moistening bud paddy.
4, preferred plant: radiation shoot survival percent is generally between 25～35%, treats that young plant grows to 5 leaves and can transplant to land for growing field crops, examines the situation recording variation subsequently, and optimum selecting has the plant of value.
5, the processing scheme of variant and enforcement: find sterile strain, then plant division is accredited as two and is or three genes being, and carries out its value analysis；The fertile plant of different qualities, backcrosses, it is thus achieved that advantage is polymerized.
Ma Di meter matter spy is excellent for Bath, and yields poorly, and paddy awn length, not anti-fall, adaptability is narrow.Through mutation, after backcrossing, select new varieties, novel sterile line, there is the advantage of anti-fall, high temperature resistant, wide adaptability, stable yields, all U.S.s of rice color, smell and taste.And select the new varieties that absorption zinc selenium function is strong.
Embodiment 2:
1, select seed-grain: screen full wild rice millet 100 seed, keep moistening accelerating germination.
2, seed pendulum shape: the round basin of what a 25～30 cm diameter standby, loads onto moistening thin sand and soil, the seed that will germinate, and every 10 is one group, and program request becomes pyramid, eight pyramids form a basin, it is simple to the record of different time radiation.
3, seed radiation: when bud paddy grows to 1～5 centimetre with magnifier focus on big sun can focus radiation bastem portion, radiation point three steps carry out: just the light focus big with Semen Viciae fabae is irradiated 30 seconds；Second step is reduced to a Semen Citrulli large radiation 30 seconds heavily to process focus；3rd step is violent process, and focus is reduced to a Semen Glycines size radiation, occurs that namely micro-emitting vaporous stop；Instil after completing two mineral waters every time, with moistening bud paddy.
4, preferred plant: radiation shoot survival percent is generally between 25～35%, treats that young plant grows to 5 leaves and can transplant to land for growing field crops, examines the situation recording variation subsequently, and optimum selecting has the plant of value.
5, the processing scheme of variant and enforcement: find sterile strain, then plant division is accredited as two and is or three genes being, and carries out its value analysis；The fertile plant of different qualities, backcrosses, it is thus achieved that advantage is polymerized.
The weakness such as wild rice has strong stress resistance, but yield is extremely low, and maturation is inconsistent.The characteristic functions such as taking to utilize the seed that grain quality rice and wild rice backcross through radiation treatment, select CMS line, the novel compositions allotted has: fragrant soft red rice, fragrant soft fructus zizaniae caduciflorae, steamed brown rice perfume (or spice) are soft, and overnight rice is not hardening.
Embodiment 3:
1, seed-grain is selected: screen full Salt And Alkali Tolerance sea water Oryza glutinosa 100 seeds, keep moistening accelerating germination.
2, seed pendulum shape: the round basin of what a 25～30 cm diameter standby, loads onto moistening thin sand and soil, the seed that will germinate, and every 10 is one group, and program request becomes pyramid, eight pyramids form a basin, it is simple to the record of different time radiation.
3, seed radiation: when bud paddy grows to 1～5 centimetre with magnifier focus on big sun can focus radiation bastem portion, radiation point three steps carry out: just the light focus big with Semen Viciae fabae is irradiated 30 seconds；Second step is reduced to a Semen Citrulli large radiation 30 seconds heavily to process focus；3rd step is violent process, and focus is reduced to a Semen Glycines size radiation, occurs that namely micro-emitting vaporous stop；Instil after completing two mineral waters every time, with moistening bud paddy.
4, preferred plant: radiation shoot survival percent is generally between 25～35%, treats that young plant grows to 5 leaves and can transplant to land for growing field crops, examines the situation recording variation subsequently, and optimum selecting has the plant of value.
5, the processing scheme of variant and enforcement: find sterile strain, then plant division is accredited as two and is or three genes being, and carries out its value analysis；The fertile plant of different qualities, backcrosses, it is thus achieved that advantage is polymerized.
Salt And Alkali Tolerance sea water rice, yield poorly, circularity is low, adaptability is narrow, paddy awn length, not resistant to lodging.Through overshoot and cool freeze Duplex treatment after, solve perhaps problem.Cultivated the two-line sterile line of Salt And Alkali Tolerance early, in, ripe late conventional Rice.Ratio former sea increasing production of rice more than 2 times, rice matter is also improved, and stem stalk is thicker strong, well developed root system, plant height about 1 meter, resistance to extensive, adapt to live.
Claims (2)
1. utilizing solar energy focus radiation bud paddy can keep the method that the variation quick advantage of strain is stable, its feature comprises the following steps:
(1) select seed-grain: screen full millet 100 seed, keep moistening accelerating germination；
(2) seed pendulum shape: the round basin of what a 25～30 cm diameter standby, loads onto moistening thin sand and soil, the seed that will germinate, and every 10 is one group, and program request becomes pyramid, eight pyramids form a basin, it is simple to the record of different time radiation；
(3) seed radiation: when bud paddy grows to 1～5 centimetre with magnifier focus on big sun can focus radiation bastem portion, radiation point three steps carry out: just the light focus big with Semen Viciae fabae is irradiated 30 seconds；Second step is reduced to a Semen Citrulli large radiation 30 seconds heavily to process focus；3rd step is violent process, and focus is reduced to a Semen Glycines size radiation, occurs that namely micro-emitting vaporous stop；Instil after completing two mineral waters every time, with moistening bud paddy；
(4) preferred plant: radiation shoot survival percent is generally between 25～35%, treats that young plant grows to 5 leaves and can transplant to land for growing field crops, examines the situation recording variation subsequently, and optimum selecting has the plant of value；
(5) processing scheme of variant and enforcement: find sterile strain, then plant division is accredited as two and is or three genes being, and carries out its value analysis；The fertile plant of different qualities, backcrosses, it is thus achieved that advantage is polymerized.
2. a kind of according to claim 1 utilizes solar energy focus radiation bud paddy can keep the method that the variation quick advantage of strain is stable, it is characterized in that: seed radiates at Qiu Mo or severe winter, it is heat to 35 degree with a steamer water, food steamer upper berth warp layer cloth, bud paddy swings on gauze, carry out radiation treatment, after the radiation of bud paddy, move into winter numerous base in greenhouse or Hainan.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852277A (en) * | 2016-12-28 | 2017-06-16 | 陵水重学辐射育种研究所 | A kind of novel method for cultivating of saline and alkaline rice sterile line |
| CN106900542A (en) * | 2017-03-31 | 2017-06-30 | 陵水重学辐射育种研究所 | A kind of method that utilization wild rice cultivates saline and alkaline rice |
| CN114731798A (en) * | 2022-04-25 | 2022-07-12 | 核工业西南物理研究院 | Method for increasing selenium content in rice and cultivation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125376A (en) * | 2013-03-28 | 2013-06-05 | 周安岳 | Method for mutating mutant strain by radiating base part of rice sprout by utilizing focus of solar energy |
| CN103141377A (en) * | 2013-03-28 | 2013-06-12 | 周安岳 | Light-sensation two-line cultivation method |
| CN103828704A (en) * | 2012-11-23 | 2014-06-04 | 田阳恒星农业科技有限公司 | Method for cultivating fine breed of hybrid rice by utilizing wild rice |
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2016
- 2016-03-10 CN CN201610134270.0A patent/CN105766628A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828704A (en) * | 2012-11-23 | 2014-06-04 | 田阳恒星农业科技有限公司 | Method for cultivating fine breed of hybrid rice by utilizing wild rice |
| CN103125376A (en) * | 2013-03-28 | 2013-06-05 | 周安岳 | Method for mutating mutant strain by radiating base part of rice sprout by utilizing focus of solar energy |
| CN103141377A (en) * | 2013-03-28 | 2013-06-12 | 周安岳 | Light-sensation two-line cultivation method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106852277A (en) * | 2016-12-28 | 2017-06-16 | 陵水重学辐射育种研究所 | A kind of novel method for cultivating of saline and alkaline rice sterile line |
| CN106900542A (en) * | 2017-03-31 | 2017-06-30 | 陵水重学辐射育种研究所 | A kind of method that utilization wild rice cultivates saline and alkaline rice |
| CN114731798A (en) * | 2022-04-25 | 2022-07-12 | 核工业西南物理研究院 | Method for increasing selenium content in rice and cultivation method |
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