CN103828617B - A kind of biological method inducing dalbergia odorifera Edgeworthia chrysantha - Google Patents
A kind of biological method inducing dalbergia odorifera Edgeworthia chrysantha Download PDFInfo
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- CN103828617B CN103828617B CN201410104193.5A CN201410104193A CN103828617B CN 103828617 B CN103828617 B CN 103828617B CN 201410104193 A CN201410104193 A CN 201410104193A CN 103828617 B CN103828617 B CN 103828617B
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- dalbergia
- heartwood
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- edgeworthia chrysantha
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Abstract
The invention belongs to biological technical field, relate to a kind of biological method inducing dalbergia odorifera Edgeworthia chrysantha, be to choose to drill through heartwood on the adult dalbergia odorifera tree of Edgeworthia chrysantha, join after pulverizing in ISP2 fluid nutrient medium and cultivate, prepare the endophyte fungus strain nutrient solution of dalbergia wood；Choosing the dalbergia odorifera tree not forming heartwood for 6～7 years, hole on trunk, hole depth, to xylem, then by endophyte fungus strain nutrient solution hand-hole, then carries out encapsulation process to aperture, after 12 months, takes mauve xylem be dalbergia wood from processing position.Present invention process is simple; easy and simple to handle; utilize the endophyte fungus strain of dalbergia wood itself to induce dalbergia wood; effectively shorten the production cycle of dalbergia odorifera Edgeworthia chrysantha; there is advantage natural, non-harmful, be one of the most promising the safest method, it is adaptable to dalbergia wood large-scale standardized, merchandized handling; be conducive to the protection of dalbergia odorifera resource, development and utilization, there are important economy, society and ecological benefits.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of method stimulating dalbergia odorifera Edgeworthia chrysantha, specifically a kind of
The method using biological method induction dalbergia odorifera Edgeworthia chrysantha.
Background technology
Chinese medicine dalbergia wood is trunk and the root of legume dalbergia odorifera (Dalbergia odorifera T.Chen)
Dry duramen, dalbergia wood mainly has promoting qi circulation and relieving pain, invigorates blood circulation, stops blooding, stops the effect such as dysentery, preventing or arresting vomiting, is clinical normal
With the primary raw material of the Chinese patent drugs such as preparation such as rosewood compound capsules, GUANXIN DANSHEN PIAN breast knot dissipation sheet, can be used for
Treatment traumatic injury, wound appearance, wrist abdomen pain, obstruction of qi in the chest shouting pain etc., have higher medical value and warp
Ji is worth.In recent years, along with the market demand is continuously increased, causing wild resource to be fell trees unlawfully seriously, resource faces
The exhausted condition even become extinct.In order to alleviate this present situation, at present, various places, south China all have artificial large area to plant
Training, but under dalbergia odorifera natural growthing condition, heartwood is formed extremely slow, and after general growth 7-8, dalbergia wood is yellow
The trunk of wingceltis just initially forms heartwood, and 20-30 just can run up to some with hyoscine, Edgeworthia chrysantha process ten
Divide very long, it is impossible to meet the medication demand of society, therefore, how to make full use of dalbergia odorifera tree and accelerate its knot
Fragrant process and increase yield, becomes current and produces upper key issue the most urgently to be resolved hurrily alleviating dalbergia wood imbalance between supply and demand.
At present, producing and naturally grow formation medicinal material mainly by dalbergia odorifera, document report environmental stimuli produces perfume
Method mainly have physics to chop wound, chemical stimulation, plant hormone induction and utilize the methods such as fungal induction.But
The heartwood endophyte fungus strain induction dalbergia odorifera utilizing dalbergia odorifera self-assembling formation quickly forms the method for heartwood not
Appear in the newspapers.
Summary of the invention
It is an object of the invention to provide a kind of biology inducing dalbergia odorifera Edgeworthia chrysantha for the deficiencies in the prior art
Method, utilizes the endophyte fungus strain of dalbergia wood itself to induce dalbergia wood, and not only the production cycle shortens, and has
Advantage natural, non-harmful, is one of the most promising the safest method, it is adaptable to the extensive standard of dalbergia wood
Change, merchandized handling, the beneficially protection of dalbergia odorifera resource, development and utilization, have important economy,
Society and ecological benefits.
The technical solution adopted in the present invention:
A kind of biological method inducing dalbergia odorifera Edgeworthia chrysantha, is the upper brill of adult dalbergia odorifera tree choosing Edgeworthia chrysantha
Core material, through surface sterilization, pulverize after join in ISP2 fluid nutrient medium, 25～30 DEG C of shaken cultivation 5～
10 days, prepare the endophyte fungus strain nutrient solution of dalbergia wood, wherein dalbergia odorifera based on mass volume ratio (g/mL)
Pith material is 1:10～50 with the ratio of ISP2 fluid nutrient medium；Choose the dalbergia wood not forming heartwood for 6～7 years
Kingwood, holes in the scope below overhead 50cm height on trunk, and hole depth is to xylem, then
By in endophyte fungus strain nutrient solution hand-hole, then aperture is carried out encapsulation process, after 12 months, from process portion
Position takes mauve xylem and is dalbergia wood.
Take part aubergine xylem, dry, after comminuted powder with appropriate high-purity distilled water immersion, with volatilization
The boiling of steam circumfluence method is not until volatile oil is further added by oil extractor routinely, dried through anhydrous sodium sulfate
Obtain volatile oil.Volatile oil is water white transparency oily thing, has special fragrant.By with reference to 2010 " in
China's people's republic pharmacopeia " determination of volatile oil method (annex XD first method) measures the content of its volatile oil, by gas
Chemical composition is identified by phase chromatography-mass spectroscopy (GC-MS), determines percentage contents by normalization method.
Present invention process is simple, easy and simple to handle, utilizes the endophyte fungus strain of dalbergia wood itself to induce dalbergia wood, has
Effect ground shortens the production cycle of dalbergia odorifera Edgeworthia chrysantha, has advantage natural, non-harmful, is the safest to have most
One of method of prospect, it is adaptable to dalbergia wood large-scale standardized, merchandized handling, beneficially dalbergia odorifera money
The protection in source, development and utilization, have important economy, society and ecological benefits.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.Hereinafter implement
Example is used for illustrating the present invention, but is not limited to the scope of the present invention.Unreceipted concrete bar in the following example
The experimental technique of part, generally according to normal condition, or according to the condition proposed by manufacturer.
Embodiment one
Within 60 years, 5g heartwood is drilled through with growth cone adult dalbergia odorifera tree, through 70% alcohol surface from one
After sterilization, pulverizing of milling under aseptic condition, put in 200mL ISP2 fluid nutrient medium, 28 DEG C, 180rpm
After shaken cultivation 1 week, prepare the endophyte fungus strain nutrient solution of dalbergia wood.Choose 6-7 and do not form the dalbergia wood of heartwood
Kingwood 30 strain, in the scope below overhead 50cm height with the drill bit of a diameter of 1cm on trunk
From top to bottom bore 3 holes (tilting 45 °), hole depth to xylem, each hole between the upper and lower be spaced apart 10～
15cm, injects 1mL nutrient solution, by preservative film and adhesive tape sealed envelope, processes 12 months in each hole
After, cut down trees, take mauve xylem from processing position.The aubergine material that will obtain, dry,
Being precisely weighed, weight is 6090g, average strain yield: 203g.
Embodiment two
Within 40 years, 3g heartwood is drilled through with growth cone adult dalbergia odorifera tree, through 70% alcohol surface from one
After sterilization, pulverizing of milling under aseptic condition, put in 100mL ISP2 fluid nutrient medium, 28 DEG C, 180rpm
After shaken cultivation 1 week, prepare the endophyte fungus strain nutrient solution of dalbergia wood.Choose 6-7 and do not form the dalbergia wood of heartwood
Kingwood 30 strain, in the scope below overhead 50cm height with the drill bit of a diameter of 1cm on trunk
From top to bottom bore 3 holes (tilting 45 °), hole depth to xylem, each hole between the upper and lower be spaced apart 10～
15cm, injects 1mL nutrient solution, by preservative film and adhesive tape sealed envelope, processes 12 months in each hole
After, cut down trees, take mauve xylem from processing position.The aubergine material that will obtain, dry,
Being precisely weighed, weight is 6420g, average strain yield: 214g.
Embodiment three
Within 70 years, 2g heartwood is drilled through with growth cone adult dalbergia odorifera tree, through 70% alcohol surface from one
After sterilization, pulverizing of milling under aseptic condition, put in 50mL ISP2 fluid nutrient medium, 28 DEG C, 180rpm
After shaken cultivation 1 week, prepare the endophyte fungus strain nutrient solution of dalbergia wood.Choose 6-7 and do not form the dalbergia wood of heartwood
Kingwood 30 strain, in the scope below overhead 50cm height with the drill bit of a diameter of 1cm on trunk
From top to bottom bore 3 holes (tilting 45 °), hole depth to xylem, each hole between the upper and lower be spaced apart 10～
15cm, injects 1mL nutrient solution, by preservative film and adhesive tape sealed envelope, processes 12 months in each hole
After, cut down trees, take mauve xylem from processing position.The aubergine material that will obtain, dry,
Being precisely weighed, weight is 6270g, average strain yield: 209g.
Inspection:
1, volatile oil content in product
The most accurately weigh the product 50g of embodiment one, two, three, with appropriate high-purity distillation water logging after pulverizing
Bubble 5h, with volatile oil extractor 100 DEG C of boiling 16h of steam circumfluence method routinely until volatile oil no longer increases
Add, through anhydrous sodium sulfate dried volatile oil.Volatile oil is water white transparency oily thing, has special delicate fragrance
Taste.By with reference to Pharmacopoeia of the People's Republic of China determination of volatile oil method (annex XD first method) mensuration in 2010
The content of its volatile oil, the results are shown in Table 1.
Table 1 volatile oil content index
| Project | Aubergine product (g) | Volatile oil (mL) | Recovery rate (mL/g) |
| Embodiment one | 50 | 0.68 | 1.36% |
| Embodiment two | 50 | 0.73 | 1.47% |
| Embodiment three | 50 | 0.64 | 1.28% |
Must not be less than 1.0% containing volatile oil according to the dalbergia wood that the Pharmacopoeia of the People's Republic of China in 2010 specifies
(mL/g) standard, the volatile oil content of the present invention preparation-obtained aubergine product meets standard.
2, the content of nerolidol in volatile oil
The volatile oil of Example one, two, three product is a little respectively, utilizes gas chromatography-mass spectrum (GC-MS)
Chemical composition is identified, determines percentage contents by normalization method.Gas chromatography-mass spectrometry analysis condition:
Chromatographic column: quartz capillary column HP-5MS (30mm × 0.25mm × 0.25mm), chromatographic column temperature programming bar
Part: initial temperature 50 DEG C, after initial temperature keeps 3min, and protects to 200 DEG C with the ramp of 5 DEG C/min
Hold 7min, carrier gas He；Split ratio 1:50；7.6522psi is pressed before post；Injector temperature: 250 DEG C;Ion
Source temperature: 230 DEG C；Ionization voltage: 70eV；Quality of scanning scope: 50～400AMU；Scan mode: EI
Source；Sweep spacing: 0.5s.Volatile oil is carried out constituent analysis, with area normalization by above-mentioned GC-MS condition
Change method measures the percentage contents of nerolidol, the results are shown in Table 2.
Constituent analysis in table 2 volatile oil
| Project | Constituent species | Nerolidol percentage contents |
| Embodiment one | 14 | 29.25% |
| Embodiment two | 14 | 30.71% |
| Embodiment three | 14 | 28.12% |
It can be seen from the results that isolate 14 kinds of components from volatile oil, record volatilization with area normalization method
The percentage contents of the nerolidol of main body of oil reaches high value, illustrates to use the present invention to prepare
Aubergine heartwood material is dalbergia wood really, meets the medicinal material standard of dalbergia wood.
The above is only the preferred embodiment of the present invention, it is noted that common for the art
For technical staff, on the premise of without departing from the technology of the present invention principle, it is also possible to make some improvement and profit
Decorations, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (1)
1. the biological method inducing dalbergia odorifera Edgeworthia chrysantha, it is characterised in that: choose the adult fall of Edgeworthia chrysantha
Drill through heartwood on fragrant kingwood, join in ISP2 fluid nutrient medium after surface sterilization, pulverizing, 25～30 DEG C
Shaken cultivation 5～10 days, prepare the endophyte fungus strain nutrient solution of dalbergia wood, wherein dalbergia wood based on mass volume ratio
Kingwood heartwood is 1:10～50 with the ratio of ISP2 fluid nutrient medium；Choose 6～7 years and do not form heartwood
Dalbergia odorifera tree, in the scope below overhead 50cm height on trunk hole, hole depth to xylem,
Then by endophyte fungus strain nutrient solution hand-hole, then aperture is carried out encapsulation process, after 12 months, from from
Reason position takes mauve xylem and is dalbergia wood.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104621186B (en) * | 2015-02-10 | 2017-11-21 | 林励 | A kind of composition, the application of the method for liquid, the induction liquid on stimulation dalbergia wood Edgeworthia chrysantha is induced by its preparation |
| CN106508557A (en) * | 2016-10-19 | 2017-03-22 | 宜宾云辰乔木园林有限责任公司 | Method for early forming heartwood by dalbergia odorifera |
| CN110605761B (en) * | 2019-08-20 | 2022-04-15 | 海南海香园投资有限公司 | Hand-held drill bit device capable of promoting trees to generate agilawood |
| CN111657042B (en) * | 2020-06-30 | 2021-12-03 | 中国林业科学研究院资源昆虫研究所 | Planting method for promoting formation of pterocarpus indicus heartwood |
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| CN102550311A (en) * | 2011-12-29 | 2012-07-11 | 中国林业科学研究院热带林业研究所 | Method for rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid |
| CN102696690A (en) * | 2012-06-15 | 2012-10-03 | 唐显 | Flora and method for producing Chinese eaglewood wood on Aquilaria senensis (Lour.) Gilg by bottle interpolation method |
| CN102726224A (en) * | 2012-04-17 | 2012-10-17 | 唐文 | Agilawood producing on aquilaria senensis gilg trees by using stick inserting method |
| CN102783349A (en) * | 2012-08-29 | 2012-11-21 | 广西壮族自治区林业科学研究院 | Method for improving breeding quality of dalbergia odorifera cuttings |
| CN102827779A (en) * | 2012-09-13 | 2012-12-19 | 广东志康药业股份有限公司 | Fungus for inducing dalbergia odorifera to form dalbergia woods |
| CN103168634A (en) * | 2013-03-26 | 2013-06-26 | 中山市沉香黄农林发展有限公司 | Method for promoting aquilaria sinensis to carry out secondary edgeworthia chrysantha |
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- 2014-03-20 CN CN201410104193.5A patent/CN103828617B/en not_active Expired - Fee Related
Patent Citations (6)
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| CN102550311A (en) * | 2011-12-29 | 2012-07-11 | 中国林业科学研究院热带林业研究所 | Method for rapid formation of agilawood of agallochum through induction by using fungus fermentation liquid |
| CN102726224A (en) * | 2012-04-17 | 2012-10-17 | 唐文 | Agilawood producing on aquilaria senensis gilg trees by using stick inserting method |
| CN102696690A (en) * | 2012-06-15 | 2012-10-03 | 唐显 | Flora and method for producing Chinese eaglewood wood on Aquilaria senensis (Lour.) Gilg by bottle interpolation method |
| CN102783349A (en) * | 2012-08-29 | 2012-11-21 | 广西壮族自治区林业科学研究院 | Method for improving breeding quality of dalbergia odorifera cuttings |
| CN102827779A (en) * | 2012-09-13 | 2012-12-19 | 广东志康药业股份有限公司 | Fungus for inducing dalbergia odorifera to form dalbergia woods |
| CN103168634A (en) * | 2013-03-26 | 2013-06-26 | 中山市沉香黄农林发展有限公司 | Method for promoting aquilaria sinensis to carry out secondary edgeworthia chrysantha |
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