CN103784497A - Composition for improving menopausal women bone and intestinal tract health and preparation method thereof - Google Patents
Composition for improving menopausal women bone and intestinal tract health and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a composition for improving menopausal women bone and intestinal tract health and a preparation method thereof. The composition is prepared from the following raw materials in parts by mass: 1-200 parts of enzymolysis bone calcium, 5-50 parts of a radix puerariae extract, 10-100 parts of fructooligosaccharide, 0-800 parts of honey, and 0-50 parts of sodium citrate. The composition alleviates a series of menopausal women syndromes, such as constipation, indigestion and the like, caused by osteoporosis and weak intestinal tract functions caused by physiological changes.
Description
Technical field
The present invention relates to a kind of compositions of improving climacteric women skeleton and intestinal health.
Background technology
Calcium is one of indispensable important element of human body, most important aspect the maintaining of the growth promoter of skeleton and normal function.No matter be budding child, climacteric women or old people, all should take in time enough calcium, thereby safeguard the stable of body calcium constituent level, guarantee skeleton normal growth, the disease that the calcium deficiency such as prevention of osteoporosis cause.According to statistics, approximately there is the sufferers of osteoporosis face that exceedes 100,000,000 in China, expects the year two thousand fifty will be increased to 200,011,000, and wherein, an important colony is climacteric women.Before and after postmenopausal women, due to hypo-ovaria, body inner estrogen level declines, and causes that the function adjustment of each organ-tissue is incompatible, occurs a series of diseases, and especially the variation of bone health situation is this crowd's of puzzlement problem.Osteoporosis refers to that osseous tissue reduces and causes skeleton crisp and frangible, easily fracture, and women after climacteric, because main cause is the rear decrease in estrogen of ovarian function decline, there is negative balance in bone metabolism, and bone amount reduces, and sickness rate is high more a lot of than male.
Mel is a kind of nutritious natural nourishing food being loved by the people, and is also one of the most frequently used tonic.Have and take conveniently, the feature that mouthfeel is good.According to one's analysis, the trace element that contains plurality of inorganic salt, multiple organic acid and the useful healths such as the ferrum close with human serum concentration, manganese, potassium, and fructose, glucose, amylase, oxidase, reductase etc., especially it is rich in multivitamin V
b, V
c, V
d, V
e, be safe vitamin supplement source, simultaneously for V
d, V
ethis type of fatsoluble vitamin, Mel has significant advantage as wholefood aspect safety.Therefore, in order to make full use of the collaborative facilitation of vitamin to calcium absorption, in bee product, strengthen the mode of calcium, can reach assurance safety, give full play to calcium supplementing effect, and obtain good taste and daily arbitrary way effect easily.
In view of the pursuit of people to bone health state and existing body calcium are taken in the contradiction of the uneven present situation running off, just impel field of health care food, to green safety, formula rationally, the continuous exploration upgrading of the good calcium supplementary of good absorbing, effect, for constantly adapting to society, bone health is pursued desirable health food is provided.Meanwhile, due to the absorption of calcium complement agent, be difficult to avoid the impact on intestinal metabolism, cause the symptom of constipation, and climacteric women be with the phenomenon of multiple constipation.Therefore, for climacteric women, provide a kind of product that solves skeleton and intestinal health, become those skilled in the art's urgent problem.
Summary of the invention
The object of the present invention is to provide a kind of compositions of improving climacteric women skeleton and intestinal health, technical scheme of the present invention is summarized as follows:
Improve a compositions for climacteric women skeleton and intestinal health, comprise that by mass fraction following raw material makes: 0～50 part of 1～200 part of enzymolysis bone calcium, 5～50 parts of Radix Puerariae extracts, 10～100 parts of oligofructoses, 0～800 part of Mel and sodium citrate.
Preferably: described enzymolysis bone calcium is that 80～160 parts, described Radix Puerariae extract are that 6～30 parts, described oligofructose are 11～50 parts, and described Mel is 200～700 parts, and described sodium citrate is 1～40 part.
Preferably described enzymolysis bone calcium is 100～150 parts, and described Radix Puerariae extract is 10～20 parts, and oligofructose is 12～15 parts, and described Mel is 300～500 parts, and described sodium citrate is 2～10 parts.
Preferably mass content >=60% of flavone in Radix Puerariae extract.Preferably mass content >=80% of flavone in Radix Puerariae extract.Described Mel is Flos Jujubae Mel, tilia Mel or Flos Sophorae honey.
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get by mass fraction: 0～50 part of 1～200 part of enzymolysis bone calcium after pulverizing, 5～50 parts of Radix Puerariae extracts, 10～100 parts of oligofructoses, 0～800 part of Mel and sodium citrate;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 2～10 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 1～10 minute;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 2-10 minute, grinds 5-15 minute in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Preferably described enzymolysis bone calcium is that 80～160 parts, described Radix Puerariae extract are that 6～30 parts, described oligofructose are 11～50 parts, and described Mel is 200～700 parts, and described sodium citrate is 1～40 part.
Preferably described enzymolysis bone calcium is 100～150 parts, and described Radix Puerariae extract is 10～20 parts, and oligofructose is 12～15 parts, and described Mel is 300～500 parts, and described sodium citrate is 2～10 parts.
Mass content >=60% of flavone in described Radix Puerariae extract; Described Mel is Flos Jujubae Mel, tilia Mel or Flos Sophorae honey.
Compositions of the present invention is alleviated the series comprehensive causing a little less than osteoporosis that climacteric women causes due to physiological change and function of intestinal canal and is levied, as constipation, dyspepsia etc.
The specific embodiment
Radix Puerariae extract used in the present invention can be buied from open market.
We are by a kind of the present invention compositions called after that improves climacteric women skeleton and intestinal health " like you prebiotic Ms's Mel ".
Below in conjunction with specific embodiment, the present invention is further illustrated.
The compositions of embodiment 1-embodiment 7 is in table 1, and each component according to the mass fraction
| ? | Enzymolysis bone calcium | Radix Puerariae extract | Oligofructose | Mel | Sodium citrate |
| Embodiment 1 | 120 | 15* | 13 | 400(Flos Jujubae Mel) | 0 |
| Embodiment 2 | 100 | 20* | 12 | 500(Flos Jujubae Mel) | 10 |
| Embodiment 3 | 150 | 10* | 15 | 300(Flos Jujubae Mel) | 2 |
| Embodiment 4 | 80 | 30 | 11 | 700(tilia Mel) | 40 |
| Embodiment 5 | 160 | 6 | 50 | 200(tilia Mel) | 1 |
| Embodiment 6 | 1 | 50 | 10 | 800(Flos Sophorae honey) | 50 |
| Embodiment 7 | 200 | 5 | 100 | 0(Flos Sophorae honey) | 5 |
[0025]in Radix Puerariae extract, add mass content>=80% of flavone in its Radix Puerariae extract of " * ", do not add mass content>=60% of flavone in " * " its Radix Puerariae extract.
Embodiment 8
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 1;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 6 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 5 minutes;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 5 minutes, grinds 10 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Embodiment 9
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 2;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 4 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 4 minutes;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 4 minutes, grinds 12 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Embodiment 10
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 3;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 5 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 5 minutes;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 5 minutes, grinds 5 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Embodiment 11
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 4;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 2 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 1 minute;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 10 minutes, grinds 15 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Embodiment 12
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 5;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 10 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 10 minutes;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 2 minutes, grinds 10 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Embodiment 13
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 5;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 6 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 5 minutes;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 5 minutes, grinds 10 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Embodiment 14
A preparation method of improving the compositions of climacteric women skeleton and intestinal health, comprises the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get each component and quality shown in embodiment 6;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 6 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 5 minutes;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 5 minutes, grinds 10 minutes in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
Test example
In order to verify the effect of a kind of compositions of improving climacteric women skeleton and intestinal health of the present invention.Carried out zoopery, method and result are as mentioned below.
1. materials and methods
1.1 tested materials: compositions of the present invention (compositions of embodiment 1, the method preparation of embodiment 8)
1.2 experimental technique
1.2.1 the impact of compositions of the present invention on osteoporosis in ovariectomized rats
1.2.1.1 laboratory animal
50 Healthy female SD rats of copulation not, 3.5 monthly ages, body weight 250 ~ 280g.Purchased from zoopery center, Chinese military medicine academy of science radiological study institute Tianjin.
1.2.1.2 laboratory animal grouping
Rat adapts to raise after one week at this laboratory, is divided at random 5 groups, 10 every group by body weight:
False operative control group (Sham): rat is carried out to sham-operation, carry out the operation process identical with ovariectomy, but do not extract ovary, the other fritter fat of a spay;
Removal ovary matched group (Ovx): surgical removal rat ovary;
Low dosage tested material group (L-TS): surgical removal rat ovary, the compositions of the present invention of feeding every day, amounting to the supplementary content of calcium is 66.7mg/kg.BW;
Middle dosage tested material group (M-TS): surgical removal rat ovary, the compositions of the present invention of feeding every day, amounting to the supplementary content of calcium is 133.3mg/kg.BW.
High dose tested material group (H-TS): surgical removal rat ovary, the compositions of the present invention of feeding every day, amounting to the supplementary content of calcium is 400mg/kg.BW.
1.2.1.3 the foundation of osteoporosis animal model
10% chloral hydrate intraperitoneal injection of anesthesia (dosage 0.3mL/100g bw) for each group rat, the position of bowing is fixed.Choose back preserved skin, iodine tincture and alcohol disinfecting processing, cut off epidermis, enter abdominal cavity, cut off muscular tissue at ovary place, the left and right sides respectively, with tweezers by ovary tractive to outside, Ligation of artery, extract both sides ovary, the other fritter fatty tissue of false operative control group spay, then sews up both sides muscle, rinses internal incision for protecting from infection with gentamycin, finally sew up back epidermal wound, partial smearing iodine tincture disinfection.In latter one week of operation, the every daily iodine disinfection of wound.
After operation, rat recovers to enter for one week formal experimental period, every 5 rat one cages, and feeding environment temperature is 20 ~ 24 ℃, 12h illumination alternately, is freely drunk water and takes food.Observe animal diet followed situation every day, require gavage tested material according to each group of dosage, weigh weekly once.
After rat feeding 3 months, fasting 24h, femoral artery blood sampling, collects blood, centrifuging and taking serum ,-20 ℃ of Refrigerator stores are for subsequent use.Rat is put to death in dislocation afterwards, separates both sides femurs, muscle and soft tissue is picked totally to be placed on-20 ℃ of Refrigerator stores for subsequent use.
1.2.1.4 the mensuration of rat femur density
With double energy X-ray borne densitometers mensuration right side femoral bmd.When measurement, rat femur is positioned on toy platform along platform major axis.Use toy software scans, scanning speed is 0.8mm/s, and sweep length is 6cm, and the coefficient of variation is 0.9%.
1.2.1.5 the mensuration of rat femur calcium content
The mensuration of bone calcium is utilized atomic absorption method.
(1) mensuration of calcium content of bone
Principle: sample, after wet method digestion, imports in atomic absorption spectrophotometer, after flame atomization, absorbs the resonance line of 422.7nm, and its absorbtivity is directly proportional to content, more quantitative with standard series.
Operating procedure:
1. sample treatment: 100 ℃ of left side femurs are dried to 1h, accurately claim dry weight, be put in respectively 100mL beaker in tall form, add nitric acid digestion liquid 10mL, upper cover table ware, soaks 20 ~ 24h.Be placed in hot digestion on the electric hot plate of 150 ℃ of left and right, digestion when light yellow, adds 0.2mL perchloric acid, continues digestion to colourless, adds several ml deionized water, and heating is to remove unnecessary nitric acid.When liquid in beaker approaches 2 ~ 3mL, take off cooling.Wash and be transferred in 100mL volumetric flask with deionized water, add the lanthanum solution 3mL of 50g/L, add deionized water standardize solution.Get and the mixed acid digestion liquid of sample digestion same amount, do reagent blank test determination by aforesaid operations.
2. measure: use liquid to prepare respectively the standard diluent of variable concentrations series (0 μ g/mL, 1 μ g/mL, 2 μ g/mL, 4 μ g/mL, 8 μ g/mL, 10 μ g/mL), Criterion curve chart standard calcium.Other experiment conditions: the fast flow of instrument slit, air and second, lamp holder height, element lamp electric current etc. are all adjusted to optimum state by the instrument explanation using.
The normal concentration series of the sample liquid having digested, reagent blank liquid and each element is imported respectively to flame to be measured.
3. calculate: calculate regression equation by calcium constituent standard diluent concentration with corresponding absorbance
C＝aA＋b
In formula: the concentration of element in C-test sample (can directly be drawn by computer μ gmL-1);
A-slope of curve;
The trap value of A-element;
The intercept of b-curve.
Drawn by regression equation or computer after the concentration of measuring sample liquid and reagent blank liquid, then by formula calculating below.
In formula: X---the content of element in sample, same to mg100g-1;
The concentration (being found by standard curve) of C---calcium in sample element, μ gmL-1;
C0---the concentration of calcium constituent (being found by standard curve) in blank solution, μ gmL-1;
V---sample constant volume, mL;
F---extension rate;
M---sample quality, g;
1.2.1.6 statistical analysis: adopt SPSS software to carry out the variance analysis of each tested material group and false operation group and removal ovary matched group.
1.2.2 the impact of compositions of the present invention (like you prebiotic Ms's Mel) on human body intestinal canal flora
1.2.2 tested crowd is through all normal 100 adult volunteers of health check-up index.It is divided into test-meal group and blank group at random, every group of 50 people, according to products characteristics and target consumer group, experimenter is mainly 40-60 year women.
1.2.3 observation index
1.2.3.1 the situation such as ordinary circumstance experimenter's spirit, diet, sleep, defecation, blood pressure.
1.2.3.2 before safety indexes test, experimenter carries out Chest X-rays, electrocardiogram, Abdominal B type ultrasonography, liver, kidney function test; Before and after test, carry out routine blood test, routine urinalysis, just conventional, blood biochemistry detection.
1.2.3.3 efficiency index bacillus bifidus, lactobacillus, enterobacteria, enterococcus, bacteroid, bacillus perfringens.
1.2.4 test method
1.2.4.1 test procedure is before giving given the test agent, and the aseptic experimenter's feces of taking, checks the quantitative indexes such as bacillus bifidus, lactobacillus, enterobacteria, enterococcus, bacteroid, bacillus perfringens.Experimenter takes " like you prebiotic Ms's Mel " every day 1 time, each 20mL, and warm water is modulated into 200mL and drinks, continuously 14d.After giving the last 24h of given the test agent, the aseptic experimenter's feces of taking, checks These parameters again.Before and after blank group crowd tests, the collection of feces is the same.
1.2.4.2 the intestinal microbial population method of inspection is collected experimenter's fresh excreta in Bedpan, censorship immediately.Quantitatively take specimen, 10 times dilution and homogenate after, be seeded to respectively each selective medium.Bacillus bifidus: 37 ℃ of BLL culture medium, 48h anaerobism are cultivated; Lactobacillus: 37 ℃ of MRS culture medium, 48h cultivate; Enterobacteria: 37 ℃ of Yihong methylene blue culture medium, 24h cultivate; Enterococcus: 37 ℃ of sodium azide-crystal violet-seven taste glycosides culture medium, 48h cultivate; Bacteroid: 37 ℃ of improvement GAM culture medium, 48h anaerobism are cultivated; Bacillus perfringens: 37 ℃ of TSC culture medium, 24h cultivate.
2. result and discussion
The impact of 2.1 tested materials on osteoporosis in ovariectomized rats
2.1.1 rat body weight growth pattern
The healing of each group rat postoperative wound is good, and without infecting, other sign ordinary circumstances are good.Five groups of rat body weights all steadily increase in time, at the 14th day five groups of mesosomes of experiment existing significant difference of reppearing, removal ovary matched group and low dosage tested material group body weight are apparently higher than false operation group, and difference has statistical significance (P<0.05, table 2); Continue higher than false operation group to testing end of term removal ovary matched group and low dosage tested material group body weight, difference has statistical significance (P<0.01), and middle high dose tested material group is compared body weight with false operative control group and significantly do not increased.
The impact of table 2 tested material on ovariectomized female rats body weight (
n=10)
Note: a is P ﹤ 005 compared with false operation group, and b is P ﹤ 0.01 compared with false operation group
2.1.2 the impact of tested material on femur of mature ovariectomized rats density and calcium content
As can be seen from Table 3, the removal ovary control rats bone density of comparing with the false operation group 12.9%(P<0.01 that significantly declines), give after various dose tested material, femur density all has in various degree and significantly improves (P<0.05 and P<0.01), and middle high dose group femur density improves respectively 5.9% and 12.1% compared with removal ovary matched group.
Compared with false operation group, the calcium content of bone of the removal ovary matched group 3.8%(P<0.01 that significantly declines), and bone calcium is significantly increased (P<0.01) after giving tested material, middle high dose group femur calcium content improves respectively 4.2% and 48% compared with removal ovary matched group.
The impact of table 3 tested material on rat femur density and calcium content (
n=10)
Note: a is P ﹤ 0.01 compared with false operation group, and b is P ﹤ 0.05 compared with removal ovary matched group, and c is P ﹤ 0.01 compared with removal ovary matched group.
BMD is the important objective index of evaluating bone loss.Calcium, phosphorus are the main composition elements of skeleton, and their excessive loss or the not good bone metabolism that all can affect of absorption, make Metabolic disorder, causes osteoporosis.After rat spay is found in this research, femur BMD is significantly lower than false operation group (P<0.01); Calcium content of bone obviously reduces (P<0.01), shows this Success in Experiment and has caused osteoporosis model.From knew that estrogen can reduce the bone loss of ovariectomized female rats research in the past, rising BMD, calcium content of bone, in this experiment, verify this point, this experiment gave tested material treatment ovariectomized group rat after 3 months, BMD, calcium content of bone significantly raise (P<0.01), show that tested material can reverse the bone loss causing because of estrogen deficiency, play the osteoporotic effect of control.
The impact of 2.2 tested materials on human body intestinal canal flora
2.2.1 the impact of tested material on experimenter's general health situation
Before test, tested crowd's spirit, sleep, diet, defecation and blood pressure are each all right; Routine blood test, just routine, the biochemical regulating liver-QI of blood, the every detection index of renal function all within normal range; Chest X-rays, electrocardiogram and Abdominal B type ultrasonography check result are normal.Duration of test, does not observe health is had to untoward reaction.After test blood, urine and just conventional index without abnormal change.
2.2.2 the impact of tested material on experimenter's intestinal microbial population
As shown in Table 4, with matched group comparison, tested group of crowd tests front each Bacteria Indicators there are no significant difference (P>0.05).Take after tested material 14d, compared with matched group, tested group of crowd's bacillus bifidus and the quantity of lactobacillus are all significance rising (P<0.01); Bacillus perfringens quantity has no remarkable increase, enterobacteria, enterococcus, bacteroid number change difference not statistically significant (P>0.05).Before and after test, self compare in process, the tested crowd of the matched group equal not statistically significant of each Bacteria Indicators difference (P>0.05).Test before and after self relatively in, tested group of crowd's bacillus bifidus and the quantity of lactobacillus significantly increase (P<0.01); Enterobacteria, enterococcus, bacteroid, the equal not statistically significant of bacillus perfringens number change difference (P>0.05).
Table 4 intestinal microbial population testing result (log cfu/g, x ± s)
* with matched group comparison, P<0101; # is relatively front with test, P<0101
Tested group of crowd takes after tested material 14d, and the variation of useful experimenter's health occurs the Microflora in intestinal; Bacillus bifidus in feces and the quantity of lactobacillus significantly increase (P<0.01); Enterobacteria, enterococcus, bacteroid and bacillus perfringens number change have no significant difference (P>0.05).This test shows, oligofructose has adjusting human body intestinal canal flora, the effect of propagation bacillus bifidus and lactobacillus, and have no adverse effects to experimenter is healthy.In health adult's intestinal, nearly more than 400 kinds of intestinal, can be divided into probiotics and harmful bacteria, and bacillus bifidus and lactobacillus are considered to two kinds of probioticss of tool physiological function in intestinal, and this class bacterium can suppress the growth of harmful bacteria.And harmful bacteria can produce harmful multiple metabolite, cause some disease.Therefore intestinal is closely related with normal microecological environment and health.Tested material produces positive effect by promoting the amount reproduction of bacillus bifidus, lactobacillus to health, as improved immunity of organisms, reducing blood fat, relieving constipation, promotes Ca
2+, Mg
2+deng mineral absorption, regulate protein metabolism, reduce blood pressure, the liver protecting, prevent the physiological functions such as diarrhoea.
Check conclusion:
Experimental results show that by above-mentioned zoopery and human body examination clothes, tested material " like you prebiotic Ms's Mel " can effectively be alleviated and suppress the relevant osteoporosis of climacteric women physiological property, and by being of value to the effect of the useful intestinal health of mode of proliferation of intestinal probiotics.
Experimental results show that: the compositions of embodiment 2-8 also can effectively be alleviated and suppress climacteric women physiological property relevant osteoporosis, and by being of value to the effect of the useful intestinal health of mode of proliferation of intestinal probiotics.
Claims (10)
1. improve a compositions for climacteric women skeleton and intestinal health, it is characterized in that comprising that by mass fraction following raw material makes: 0～50 part of 1～200 part of enzymolysis bone calcium, 5～50 parts of Radix Puerariae extracts, 10～100 parts of oligofructoses, 0～800 part of Mel and sodium citrate.
2. a kind of compositions of improving climacteric women skeleton and intestinal health according to claim 1, it is characterized in that described enzymolysis bone calcium is that 80～160 parts, described Radix Puerariae extract are that 6～30 parts, described oligofructose are 11～50 parts, described Mel is 200～700 parts, and described sodium citrate is 1～40 part.
3. a kind of compositions of improving climacteric women skeleton and intestinal health according to claim 2, it is characterized in that described enzymolysis bone calcium is 100～150 parts, described Radix Puerariae extract is 10～20 parts, oligofructose is 12～15 parts, described Mel is 300～500 parts, and described sodium citrate is 2～10 parts.
4. according to a kind of compositions of improving climacteric women skeleton and intestinal health described in claim 1,2 or 3, it is characterized in that mass content >=60% of flavone in described Radix Puerariae extract.
5. a kind of compositions of improving climacteric women skeleton and intestinal health according to claim 4, is characterized in that mass content >=80% of flavone in Radix Puerariae extract.
6. according to a kind of compositions of improving climacteric women skeleton and intestinal health described in claim 1,2 or 3, it is characterized in that described Mel is Flos Jujubae Mel, tilia Mel or Flos Sophorae honey.
7. improve a preparation method for the compositions of climacteric women skeleton and intestinal health, it is characterized in that comprising the steps:
(1) get enzymolysis bone meal and pulverize, shook 60 mesh sieves, then pulverized 60 mesh sieves;
(2) get by mass fraction: 0～50 part of 1～200 part of enzymolysis bone calcium after pulverizing, 5～50 parts of Radix Puerariae extracts, 10～100 parts of oligofructoses, 0～800 part of Mel and sodium citrate;
(3) enzymolysis bone calcium, Radix Puerariae extract and sodium citrate after described pulverizing are put into a container, stir 2～10 minutes;
(4) oligofructose, Mel are put into another container, and add pure water, stir 1～10 minute;
(5) mixture (3) and (4) being obtained mixes, and continues to stir 2-10 minute, grinds 5-15 minute in colloid mill 200 orders, and 80 eye mesh screens filter, and to obtain final product.
8. method according to claim 7, is characterized in that described enzymolysis bone calcium is that 80～160 parts, described Radix Puerariae extract are that 6～30 parts, described oligofructose are 11～50 parts, and described Mel is 200～700 parts, and described sodium citrate is 1～40 part.
9. method according to claim 8, is characterized in that described enzymolysis bone calcium is 100～150 parts, and described Radix Puerariae extract is 10～20 parts, and oligofructose is 12～15 parts, and described Mel is 300～500 parts, and described sodium citrate is 2～10 parts.
10. method according to claim 7, is characterized in that mass content >=60% of flavone in described Radix Puerariae extract; Described Mel is Flos Jujubae Mel, tilia Mel or Flos Sophorae honey.
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210429103.0A Pending CN103784497A (en) | 2012-10-31 | 2012-10-31 | Composition for improving menopausal women bone and intestinal tract health and preparation method thereof |
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| CN (1) | CN103784497A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104223304A (en) * | 2014-09-30 | 2014-12-24 | 无锡康顿生物科技有限公司 | Product capable of relieving nerves and improving menopausal symptoms and preparation method thereof |
| CN110381971A (en) * | 2017-03-07 | 2019-10-25 | 株式会社Lg生活健康 | Composition is used in the improvement of Menopause symptom |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543857A (en) * | 2003-11-13 | 2004-11-10 | 上海奥医高科技实业有限公司 | Health food having functions of postponing senescence and increasing bone mineral density |
-
2012
- 2012-10-31 CN CN201210429103.0A patent/CN103784497A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543857A (en) * | 2003-11-13 | 2004-11-10 | 上海奥医高科技实业有限公司 | Health food having functions of postponing senescence and increasing bone mineral density |
Non-Patent Citations (4)
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| 胡学智等: ""低聚果糖的生理功能及生产、应用"", 《中国食品添加剂》 * |
| 郑星季: "《天狮 一个直销企业的崛起》", 28 February 2005 * |
| 马丹丹等: ""酶解骨粉对大鼠钙吸收及骨密度的影响"", 《卫生研究》 * |
| 黄彤: ""葛根提取物对更年期骨质疏松症的功能性研究"", 《中国优秀博硕士学位论文全文数据库（硕士）医药卫生科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104223304A (en) * | 2014-09-30 | 2014-12-24 | 无锡康顿生物科技有限公司 | Product capable of relieving nerves and improving menopausal symptoms and preparation method thereof |
| CN104223304B (en) * | 2014-09-30 | 2015-10-28 | 无锡康顿生物科技有限公司 | A kind of spirit can the product that menopause symptom be improved and preparation method thereof of releiving |
| CN110381971A (en) * | 2017-03-07 | 2019-10-25 | 株式会社Lg生活健康 | Composition is used in the improvement of Menopause symptom |
| US11026987B2 (en) | 2017-03-07 | 2021-06-08 | Lg Household & Health Care Ltd. | Composition for remedying female climacteric syndrome symptoms |
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