CN103766037B - The germination method of the real seed of the worm of falling lanceolata - Google Patents
The germination method of the real seed of the worm of falling lanceolata Download PDFInfo
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- CN103766037B CN103766037B CN201410025853.0A CN201410025853A CN103766037B CN 103766037 B CN103766037 B CN 103766037B CN 201410025853 A CN201410025853 A CN 201410025853A CN 103766037 B CN103766037 B CN 103766037B
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Abstract
The invention discloses the germination method of the real seed of a kind of worm of falling lanceolata, seed germination situation under process (4 kinds) is abolished by different substrates (2 kinds), not equality of temperature condition (4 alternating temperatures), different soils water content (6 gradients) and different seed dormancy, wherein sandy soil matrix, 0/10 DEG C of (Low Night Temperature/daytime high temperature, 14h dark/10h illumination) 8% soil moisture content that relents is the ecological factor being conducive to seed germination, germination rate is respectively: 53.33%, 53.33%, 71.67%.Seed carries out after 4 kinds of dormancy abolish mode process, sprouting under the condition that the ecological factors such as matrix, temperature and soil moisture content are optimum, and it is the highest that result is that processing mode seed germination rate is abolished in the dormancy puncturing germinal aperature seed coat, is 93%.The invention solves the technical problem that the real seed germination rate of the worm of falling lanceolata is low.For husky district utilize these species to carry out revegetation, desert treatment aspect can both play a positive role.
Description
Technical field
The present invention relates to plant seed germination field, specifically relate to the method improving the real seed germination rate of the Desert Regions therophytes worm of falling lanceolata.
Background technology
Desertization has become our times and has faced the most serious one of ecotope and socioeconomic problem, and it hinders the existence of the mankind and the development of society greatly.China is one of country being subject to desertification hazard the most serious in the world, and desertization area has reached 2,620,000 km
2, account for 27% of area, and the situation in expansion.Desert treatment is extremely urgent.So select to be suitable for desert growth, the plant of strong stress resistance is for sand dune fixation, and the harm of control sand dune is significant.
The worm of falling lanceolata real (CorispermumLehmannianum) also claims precocious worm real, belong to Chenopodiaceae (Chenopodiaceace), the real genus of worm (Corispermum.) annual Spring Ephemerals, the normal 10-30cm of plant height, the flowering fruit bearing stage 4-6 month.Primary growth, at active dune lee face or sand ground, belongs to sand-fixation plant.All there are distribution in the Soviet Union, Afghanistan, Iran, at China's integrated distribution at South Border of Junggar Basin, Xinjiang, china ancient capital Xi'an.Find that these species have following characteristics by being engaged in national support items problem " Junggar Basin psammophytes restoration and reconstitution technical research and demonstration " investigation contrast in nearly 5 years during our 11th Five-Year: the ecological functions that (1) checks winds and fixes drifting sand are remarkable, be grown on the sand dune activating area divided middle and upper part, sand dune, and distribution is extensive, and importance value is only second to desert constructive species haloxylon persicum.(2) strong stress resistance, comprise high temperature resistant, weatherproof, drought-resistant, impoverishment tolerant and low temperature resistant etc., therefore the worm of falling lanceolata is the psammophytes having Development volue in fact.
The worm of falling lanceolata is real relies on seminal propagation, so grasping its seed germination technology is the prerequisite developed.But the same with eremophytes, there is " two bet " germination decision in this species seed germination, namely single germination rate is not high, and sprout time interval is long, and the harm avoiding precipitation to fluctuate to germination seed survival rate, therefore must improve seed germination rate.
" Acta Ecologica Sinica " [J] the 31st volume the 15th phase in 2011, deliver " Germination characteristics of ancient capital Xi'an and periphery 52 Plants seed and ecological significances " such as Zhang Huiliang; " Deserts in China " [J] the 32nd volume the 2nd phase in 2012, deliver " pre-tests of ancient capital Xi'an south edge 32 kinds of chenopod Germination Strategies " such as Liu Huiliang.In above-mentioned article, duration of experiment reaches 60d.But the seed germination rate of the worm of falling lanceolata reality is 0%.
Summary of the invention
The object of the present invention is to provide the method for the real seed germination of a kind of promotion worm of falling lanceolata, be convenient to better utilize these species in desert vegetation recovery afterwards, desert treatment, the ecotope of protection desert area.
To achieve these goals, the technical solution adopted in the present invention is as follows, promote a method for the real seed germination of the worm of falling lanceolata, comprise different substrates (2 kinds), different temperature match curing conditions (4 gradients), different soils water content (6 gradients) and different modes seed dormancy and abolish process (4 kinds).Concrete steps comprise:
1 seed disinfection process: first soak 10min with NaOCl reagent, then repeatedly rinse 5-6 time with distilled water;
The seed of disinfecting is placed in filter paper and sandy soil matrix by 2 respectively.Described filter paper and sandy soil matrix, be preferably sandy soil matrix.
Seed in 2 kinds of different matrix is placed in 4 alternating temperatures by 3: 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination, lower with), carry out seed germination under 5/15 DEG C, 10/25 DEG C and 15/30 DEG C of condition.4 described temperature match curing conditions, are preferably 0/10 DEG C.
4, under optimum soil matrix sandy soil and Optimal Temperature 0/10 DEG C of temperature match curing conditions, carry out 6 different soils water content: 2%, 4%, 8%, 12%, 18%, 24%, the seed germination under condition, are preferably 8%.
5 at sandy soil matrix, 0/10 DEG C of (Low Night Temperature/daytime high temperature, 14h dark/10h illumination) the ecological factor optimum such as variation, 8% soil moisture content condition under, carry out the seed germination that process is abolished in different modes dormancy, process is abolished in the dormancy that the seed that described process is disinfected carries out following 4 kinds of different modes:
(1) liquid nitrogen process seed: by the seed liquid nitrogen process 5min disinfected;
(2) seed of disinfecting is carried out cold stratification: 4 DEG C of refrigerator and cooled lamination 30-50d;
(3) sand paper galls seed coat: by the seed of disinfecting, shinny to seed coat with the sand paper seed coat that rubs gently;
(4) remove the sub-fraction seed coat in seed germination hole with sewing-needle, do not injure the Fetal liver cells of seed.
Above-mentioned 4 kinds of dormancy are abolished in processing mode, and preferred processing mode is the seed coat puncturing germinal aperature with sewing-needle.
The germination method of the real seed of the worm of falling lanceolata of the present invention, the result being abolished process by different substrates, different temperatures, different soils water content and different modes seed dormancy is: under 2 kinds of different substrates conditions, and sandy soil matrix germination rate is up to 53.33%; Under 4 different temperature match curing conditions, under the temperature match curing conditions of 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination), germination rate is up to 53.33%; Under 6 different soils Water Content Conditions, the soil moisture content germination rate of 8% is up to 71.67%; 4 kinds of different dormancy are abolished in mode process, and puncture germinal aperature seed coat with sewing-needle, germination rate is up to 93%.The invention solves the technical problem that the real seed germination rate of the worm of falling lanceolata is low, carry out for utilizing these species in husky district can both playing a positive role in revegetation, desert treatment etc.
Specific implementation method
The present invention adopts the method promoting the real seed germination of the worm of falling lanceolata to be object
Embodiment 1
1.1 seed collections: in mid-June, 2013, when ancient capital Xi'an major part seed maturity, gathers in the crops seed.The sex character of seed maturity is plant cauline leaf yellowing and soon dries up, and wherein the color of seed is brown.Select healthy and strong plant that whole strain is loaded plastic sack and take back laboratory, at room temperature airing 2 weeks.Until plant becomes dry completely, shake off seed with hand rolling, remove foreign material, being dried in the shade to water content as the place ventilated by clean seed is less than 15%.
1.2 seed room storage: when seed moisture content is less than 15%, are contained in paper bag and store under room temperature.Storage room temperature 13-25 DEG C, relative moisture <30%.
1.3 seed disinfection process: the NaOCl reagent with 1% soaks 10min, then repeatedly rinses 5-6 time with distilled water.
1.4 different substrates seed germinations: the seed of disinfecting is placed in respectively each 3 repetitions of culture dish as the 9cm of matrix of filter paper and sandy soil, each repetition 20 seeds.At 4 temperature match curing conditions: 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination, lower with), carry out seed germination in the illumination box of 5/15 DEG C, 10/25 DEG C and 15/30 DEG C, continue 70d.Seed on filter paper germination rate under 4 alternating temperatures is 0%, and the germination rate under 4 alternating temperatures of the seed in sandy soil is respectively: 53.33%, 40%, 23.33%, 21.67%.
Embodiment 2
2.1 seed collections: in mid-June, 2013, when ancient capital Xi'an major part seed maturity, gathers in the crops seed.The sex character of seed maturity is plant cauline leaf yellowing and soon dries up, and wherein the color of seed is brown.Select healthy and strong plant that whole strain is loaded plastic sack and take back laboratory, at room temperature airing 2 weeks.Until plant becomes dry completely, shake off seed with hand rolling, remove foreign material, being dried in the shade to water content as the place ventilated by clean seed is less than 15%.
2.2 seed room storage: when seed moisture content is less than 15%, are contained in paper bag and store under room temperature.Storage room temperature 13-25 DEG C, relative moisture <30%.
2.3 seed disinfection process: the NaOCl reagent with 1% soaks 10min, then repeatedly rinses 5-6 time with distilled water.
2.4 different temperatures seed germinations: the seed of disinfecting is placed in the 9cm culture dish being lined with sandy soil.Different thermogrades is set, 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination, lower with), the different alternating temperature of 5/15 DEG C, 10/25 DEG C and 15/30 DEG C etc. 4.Each process 3 repetition, each repetition 20 seeds are that illumination box is cultivated, and continue 70d.Result is that 0/10 DEG C of germination rate is up to 53.33%, and 5/15 DEG C of germination rate is 40%, and 10/25 DEG C and 15/30 DEG C of germination rate are all lower than 25%.Therefore 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination) temperature is that 4 thermograde kinds are conducive to the real seed germination of the worm of falling lanceolata most.
Embodiment 3
3.1 seed collections: in mid-June, 2013, when ancient capital Xi'an major part seed maturity, gathers in the crops seed.The sex character of seed maturity is plant cauline leaf yellowing and soon dries up, and wherein the color of seed is brown.Select healthy and strong plant that whole strain is loaded plastic sack and take back laboratory, at room temperature airing 2 weeks.Until plant becomes dry completely, shake off seed with hand rolling, remove foreign material, being dried in the shade to water content as the place ventilated by clean seed is less than 15%.
3.2 seed room storage: when seed moisture content is less than 15%, are contained in paper bag and store under room temperature.Storage room temperature 13-25 DEG C, relative moisture <30%.
3.3 seed disinfection process: the NaOCl reagent with 1% soaks 10min, then repeatedly rinses 5-6 time with distilled water.
3.4 soil different moisture content seed germinations (each process 3 repetition, each repetition 20 seeds): add 50g sandy soil in 9cm culture dish, be arranged in culture dish by neat for 20, the seed of sterilization, cover seed with 0.5cm sandy soil.Then add the distilled water of 1ml, 2ml, 4ml, 6ml, 9ml and 12ml respectively, make soil moisture content be respectively 2%, 4%, 8%, 12%, 18%, 24%.The illumination box finally culture dish of different moisture content being placed in 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination) carries out seed germination, continues 70d.Result be 8% soil moisture content seed germination rate be up to 71.67%, other gradients are all lower than 65%.
Embodiment 4
4.1 seed collections: in mid-June, 2013, when ancient capital Xi'an major part seed maturity, gathers in the crops seed.The sex character of seed maturity is plant cauline leaf yellowing and soon dries up, and wherein the color of seed is brown.Select healthy and strong plant that whole strain is loaded plastic sack and take back laboratory, at room temperature airing 2 weeks.Until plant becomes dry completely, shake off seed with hand rolling, remove foreign material, being dried in the shade to water content as the place ventilated by clean seed is less than 15%.
4.2 seed room storage: when seed moisture content is less than 15%, are contained in paper bag and store under room temperature.Storage room temperature 13-25 DEG C, relative moisture <30%.
4.3 seed disinfection process: the NaOCl reagent with 1% soaks 10min, then repeatedly rinses 5-6 time with distilled water.
Mode (each process 3 repetition, each repetition 20 seeds) is abolished in 4.4 different dormancy:
4.4.1 liquid nitrogen process seed: by after the seed liquid nitrogen process 5min that disinfected, as for being lined with in the culture dish of sandy soil, soil moisture content is 8%, in 0/10 DEG C of (Low Night Temperature/daytime high temperature, 14h dark/10h illumination) illumination box in carry out seed germination, continue 70d.Germination rate is 81.11%.
4.4.2 cold stratification seed: the seed of disinfecting is carried out following cold stratification respectively: 4 DEG C of refrigerator and cooled lamination 30d, 4 DEG C of refrigerator and cooled lamination 50d, by seed complete for cold stratification, be placed in the culture dish being lined with sandy soil, soil moisture content is 8%, in the illumination box of 0/10 DEG C (14h dark/10h illumination), carry out seed germination, continue 70d.Result is: the germination rate that cooling layer amasss 30d is 78.40%, and cooling layer amasss 50d germination rate up to 80%.
4.4.4 sand paper galls seed coat: by the seed of disinfecting, shinny to seed coat with the sand paper seed coat that rubs gently.Then, as being lined with in the culture dish of sandy soil, soil moisture content is 8%, carries out seed germination, continue 70d in the illumination box of 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination).Germination rate reaches 86.67%.
4.4.3 germinal aperature seed coat is punctured: after the seed of disinfecting is soaked 24h in distilled water, taking-up filter paper blots the water of the surface of the seed, then with the sub-fraction seed coat removing seed germination hole that sewing-needle is careful, the Fetal liver cells not injuring seed is made sure to keep in mind.Then, the seed that germinal aperature seed coat punctures is placed in the culture dish being lined with sandy soil, soil moisture content is 8%, carries out seed germination, continue 70d in the illumination box of 0/10 DEG C (Low Night Temperature/daytime high temperature, 14h dark/10h illumination).Germination rate reaches 93%.
Above-mentioned embodiment is intended to illustrate that the present invention can be professional and technical personnel in the field and realizes or use; modifying to above-mentioned embodiment will be apparent for those skilled in the art; therefore the present invention includes but be not limited to above-mentioned embodiment; any these claims or specification of meeting describes; meet and principle disclosed herein and novelty, the method for inventive features, technique, product, all fall within protection scope of the present invention.
Claims (1)
1. a germination method for the real seed of the worm of falling lanceolata, concrete steps comprise:
1) seed collection: results mature seed, the sex character of seed maturity is plant cauline leaf yellowing and soon dries up, wherein the color of seed is brown, concrete grammar is select healthy and strong plant that whole strain is loaded plastic sack to take back laboratory, at room temperature airing 2 weeks, until plant becomes dry completely, shakes off seed with hand rolling, remove foreign material, being dried in the shade to water content as the place ventilated by clean seed is less than 15%;
2) seed room storage: when seed moisture content is less than 15%, is contained in paper bag and stores under room temperature, storage room temperature 13-25 DEG C, relative moisture <30%;
3) seed disinfection process: the NaOCl reagent with 1% soaks 10min, then repeatedly rinses 5-6 time with distilled water;
4) dormancy is abolished: after the seed of disinfecting is soaked 24h in distilled water, taking-up filter paper blots the water of the surface of the seed, then with the sub-fraction seed coat removing seed germination hole that sewing-needle is careful, make sure to keep in mind the Fetal liver cells not injuring seed, then, the seed that germinal aperature seed coat punctures is placed in the culture dish being lined with sandy soil, soil moisture content is 8%, 0 DEG C/daytime 10 DEG C at night, carry out seed germination in the illumination box of 14h dark/10h illumination, continue 70d.
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| CN106234146A (en) * | 2016-09-28 | 2016-12-21 | 合肥沁牧生态农业有限公司 | A kind of implantation methods of ligustrum lucidum ait |
| CN108243653B (en) * | 2017-12-21 | 2021-03-02 | 深圳市铁汉生态环境股份有限公司 | Method for treating Trifolium repens seeds |
| CN111713205B (en) * | 2020-06-18 | 2021-10-26 | 内蒙古自治区农牧业科学院 | Lodging-resistant planting method for wood skin |
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| CN101911874A (en) * | 2010-07-28 | 2010-12-15 | 江苏丘陵地区镇江农业科学研究所 | Device for breaking dormancy of hard seeds of round leaf morning glories and method for promoting germination |
| CN102972118A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Magnolia biloba seed treatment method |
| CN103039249A (en) * | 2013-01-09 | 2013-04-17 | 甘肃省治沙研究所 | Cultivation method of seedlings of Corispermum patelliforme |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101911874A (en) * | 2010-07-28 | 2010-12-15 | 江苏丘陵地区镇江农业科学研究所 | Device for breaking dormancy of hard seeds of round leaf morning glories and method for promoting germination |
| CN102972118A (en) * | 2012-11-05 | 2013-03-20 | 溧阳市天目湖保健品有限公司 | Magnolia biloba seed treatment method |
| CN103039249A (en) * | 2013-01-09 | 2013-04-17 | 甘肃省治沙研究所 | Cultivation method of seedlings of Corispermum patelliforme |
Non-Patent Citations (1)
| Title |
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| 碟果虫实种子萌发对策及生态适应性;刘有军等;《生态学报》;20101231;第30卷(第24期);第6912页第1部分 * |
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