CN103751031A - Mouthwash containing antimicrobial peptide and sodium copper chlorophyllin and preparation method of mouthwash - Google Patents
Mouthwash containing antimicrobial peptide and sodium copper chlorophyllin and preparation method of mouthwash Download PDFInfo
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- CN103751031A CN103751031A CN201410010331.3A CN201410010331A CN103751031A CN 103751031 A CN103751031 A CN 103751031A CN 201410010331 A CN201410010331 A CN 201410010331A CN 103751031 A CN103751031 A CN 103751031A
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Abstract
The invention discloses mouthwash containing antimicrobial peptide and sodium copper chlorophyllin and a preparation method of the mouthwash, and belongs to the field of wash products. The mouthwash containing ntimicrobial peptide and sodium copper chlorophyllin comprises the following components in parts by mass: 2 to 10 parts of antimicrobial peptide, 0.001 to 0.5 part of sodium copper chlorophyllin, 0.01 to 0.05 parts of menthol, 0.01 to 0.2 part of gallic acid, 0.05 to 0.1 part of borneol, 5 to 8 parts of 95% ethanol, 0.02 to 0.2 part of essence, and 100 parts of deionized water. According to the mouthwash, sodium copper chlorophyllin is the product produced by using silkworm excrement as the raw material, has the effects of inhibiting bacteria and deodorizing, and is safe and out of toxic and side effects. The mouthwash can keep the mouth clean, inhibit harmful microbe and deodorization, and is optimal in formula, easy to prepare, and relatively low in cost.
Description
Technical field
The invention belongs to and do the wash product scope, be specifically related to a kind of collutory containing antibacterial peptide and sodium copper chlorophllin and preparation method thereof.
Background technology
In city, the collutory of oral cavity health is various in style at present, searches the open material of related products explanation and patent of invention, is mainly that Chinese medicine and chemical reagent are main.As Chinese crude drug has mentha leave, Folium Citri Limoniae, Herba Eupatorii, Radix Artemisia ordosicae, Folium Perillae, Flos Chrysanthemi Indici, Azadirachta Indica A. seed, Oleum Camelliae, Semen Maydis wet goods; Chemicals has nitre azoles, Borax, dioxy chlorine, nano-chitosan etc.In Chinese medicine extraction, need to use a large amount of organic solvents.The effective ingredient of extract and concrete content are difficult to determine, production equipment is many, and cost is higher is its weakness.
Prior art is consulted and is found the open CN101006972 of patent, and " cleaning agent for mouth cavity and preparation method thereof ", take mentha leave, Folium Citri Limoniae is raw material, uses organic solvent extraction chlorophyll.According to data mentha leave, Folium Citri Limoniae (fresh material) moisture content more than 80%, chlorophyll approximately 0.02%.Being the main component of natural solid aromatic accordingly, is indefinite with 0-3 gram of chlorophyll in its formula.Containing chlorophyll, how much do not illustrate actually yet.Certainly will be because heating be by Destruction of Chlorophyll when drying, so the content of ultimate constituent Determination of Chlorophyll is unstable.
Chinese Patent Application No. is 201210484228.3 to have announced a kind of chlorophyll collutory and preparation method thereof, and for effects such as sterilization, antiinflammatory, clean implications, but the chlorophyll adding is unstable, and fungistatic effect is not obvious, is difficult to give collutory multi-efficiency.Therefore the stable and significant fungistatic effect of seeking collutory Determination of Chlorophyll becomes problem urgently to be resolved hurrily.
Summary of the invention
For overcoming the shortcoming and defect of prior art, primary and foremost purpose of the present invention is to provide a kind of collutory containing antibacterial peptide and sodium copper chlorophllin.Use optimization formula to produce collutory, have that keep a public place clean in oral cavity, the novel mouth wash liquid of the function of antibacterial and antihalitosis.
The preparation method that the object of this invention is to provide a kind of collutory containing antibacterial peptide and sodium copper chlorophllin.
Object of the present invention realizes by following technical scheme: a kind of collutory containing antibacterial peptide and sodium copper chlorophllin, and its constituent mass percentage composition is:
Described antibacterial peptide (Antibacterial peptide) is preferably the antibacterial peptide DC in patent 200510032743.8, and the preferred activity of antibacterial peptide is 3000～6000IU/ml;
The preparation method of the above-mentioned collutory containing antibacterial peptide and sodium copper chlorophllin, concrete steps are:
(1) first Mentholum, Borneolum Syntheticum and essence are dissolved in the ethanol of volume fraction 95%;
(2) successively antibacterial peptide, sodium copper chlorophllin and gallic acid are dissolved in 20～50 parts of deionized waters, stir;
(3) solution of preparation in step (1) and step (2) is mixed, adds deionized water to 100 part, stir, standing, filter, after must contain the collutory of antibacterial peptide and sodium copper chlorophllin.
The present invention has following advantage and effect with respect to prior art:
1. antibacterial peptide of the present invention is the natural antimicrobial substance that derives from silkworm, Antherea pernyi Guerin-Meneville, Apis and batrachia, and structure is clear and definite, and cost is low.
2. in collutory of the present invention, antibacterial peptide used can, by the antibacterial effect having reached on a small quantity, with respect to other antibacterial penicillins, metronidazole and nysfungin, have good fungistatic effect.
3. chlorophyll (chlorophyll) is extensively present in green plants, and rich content is the important substance that life is depended on for existence, chlorophyll belongs to magnesium porphyrin ring-type complex, be generally fat-solublely, not soluble in water, chlorophyll itself is extremely unstable to chemical factors such as light, heat, acid, alkali, enzymes.Its derivant is relatively stable.Chlorophyll is processed into sodium copper chlorophllin, steady quality, cost is low; In the present invention, applied sodium copper chlorophllin can be good at improving the effect of halitosis.
4. gallic acid used in collutory of the present invention, has anti-bacteria and anti-virus, effectively prevents the effect of gingival hemorrhage.
5. Borneolum Syntheticum used in collutory of the present invention, has heat-clearing and fire-dispersing, drives away the function of tobacco and wine abnormal flavour.
Accompanying drawing explanation
Fig. 1 is that the collutory of antibacterial peptide and embodiment 1 is to escherichia coli E.coli K
12d
31fungistatic effect figure.Wherein, CK+: antibacterial peptide DC (5000IU/ml); CK-: the collutory that does not contain the sodium copper chlorophllin of antibacterial peptide; Sample: containing the collutory (100IU/ml) of antibacterial peptide and sodium copper chlorophllin.
The specific embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
Containing a collutory for antibacterial peptide and sodium copper chlorophllin, its constituent mass percentage composition is as table 1:
In table 1 embodiment 1, contain the composition (mass parts meter) of the collutory of antibacterial peptide and sodium copper chlorophllin
Wherein said antibacterial peptide is from the antibacterial peptide DC in patent 200510032743.8, and the activity of antibacterial peptide is 5000IU/ml;
The compound method of collutory is:
(1) first the Mentholum of 0.01 part, 0.08 part of Borneolum Syntheticum and 0.2 part of essence are dissolved in volume fraction 95% ethanol of 8 parts;
(2) successively 2 parts of antibacterial peptide DCs (5000IU/ml), 0.5 part of sodium copper chlorophllin and 0.2 part of gallic acid are dissolved in 20 parts of deionized waters, stir;
(3) step (1) is mixed with the solution of preparation in step (2), add deionized water to 100 part, stir, standing, after filtration, obtain finished product.
Embodiment 2
Containing a collutory for antibacterial peptide and sodium copper chlorophllin, its constituent mass percentage composition is as table 2:
In table 2 embodiment 2, contain the composition (mass parts meter) of the collutory of antibacterial peptide and sodium copper chlorophllin
The compound method of collutory is:
(1) first the Mentholum of 0.02 part, 0.1 part of Borneolum Syntheticum and 0.03 part of essence are dissolved in volume fraction 95% ethanol of 6 parts;
(2) successively 5 parts of antibacterial peptide DCs (5000IU/ml), 0.1 part of sodium copper chlorophllin and 0.15 part of gallic acid are dissolved in 50 parts of deionized waters, stir;
(3) step (1) is mixed with the solution of preparation in step (2), add deionized water to 100 part, stir, standing, after filtration, obtain finished product.
Embodiment 3
Containing a collutory for antibacterial peptide and sodium copper chlorophllin, its constituent mass percentage composition is as table 3:
In table 3 embodiment 3, contain the composition (mass parts meter) of the collutory of antibacterial peptide and sodium copper chlorophllin
The compound method of collutory is:
(1) first the Mentholum of 0.05 part, 0.05 part of Borneolum Syntheticum and 0.02 part of essence are dissolved in volume fraction 95% ethanol of 5 parts;
(2) successively 10 parts of antibacterial peptide DCs (5000IU/ml), 0.001 part of sodium copper chlorophllin and 0.01 part of gallic acid are dissolved in 50 parts of deionized waters, stir;
(3) step (1) is mixed with the solution of preparation in step (2), add deionized water to 100 part, stir, standing, after filtration, obtain finished product.
Detect the fungistatic effect of embodiment 1 antibacterial peptide and collutory
Antibacterial peptide DC (antibacterial peptide DC in patent 200510032743.8, the activity of antibacterial peptide is 5000IU/ml; ) and embodiment 1 make (containing antibacterial peptide and sodium copper chlorophllin) collutory to escherichia coli Escherichia.coli K
12d
31(this bacterial strain is so kind as to give by professor Huang Ziran of Agricultural University Of South China, see document: Deng little Juan, yellow nature. the separation and purification of Antibacterial Peptide and bactericidal effect [J]. Agricultural University Of South China's journal, 2002,23(3): 13-16) carry out dull and stereotyped bacteriostatic test, result as shown in Figure 1.
The collutory that contains antibacterial peptide DC is to escherichia coli E.coli K
12d
31there is good bacteriostasis.
Detect embodiment 2 collutory deodorizing effects
Not yet there is deodorization standard in China, uses the standard of the direct olfactory sensation method of the SCCNFP of European Union, by halitosis, divides Pyatyi.One-level, without halitosis; The very slight halitosis of secondary; Three grades of slight halitosis; Level Four moderate halitosis; The strong halitosis of Pyatyi.
Antihalitosis evaluation test
By 20 volunteers, be equally divided into two groups, 1 group of formula by the embodiment of the present invention 2,2 groups of formulas by the embodiment of the present invention 2, the component of removing sodium copper chlorophllin, compares.Test front two groups of volunteers and drink the medicated beer into 150ml, a Bulbus Allii fried shredded pork and two small steamed buns.With cold boiled water, gargle after the meal, by commenting fragrant teacher to detect halitosis, as benchmark.After 1 hour, with 1 group and 2 groups of collutory, gargle 30 seconds, have a rest 30 minutes.By commenting fragrant teacher to evaluate halitosis level, the evaluation result using result as antihalitosis, in Table 7 again.
Table 7 collutory deodorizing effect is evaluated
By first, comment fragrant teacher to evaluate for 1 group; By second, comment fragrant teacher to evaluate for 2 groups.
Halitosis index calculation method: the meansigma methods of ∑ stink rank X number of cases.
The indication of 3 groups of data, halitosis index is higher after the meal, the deodorizing effect of check plot (without sodium copper chlorophllin collutory) with organize before the meal indifference.With the formula of embodiment 2, after gargling, deodorization index is (7.0+6.6)/2 in 6.8(table 7), three groups of comparisons, p < 0.05, has more dominant difference.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (3)
1. containing a collutory for antibacterial peptide and sodium copper chlorophllin, it is characterized in that its constituent mass percentage composition is:
2. the collutory containing antibacterial peptide and sodium copper chlorophllin according to claim 1, is characterized in that: described antibacterial peptide is the antibacterial peptide DC in patent 200510032743.8, and the activity of antibacterial peptide is 3000～6000IU/ml.
3. the preparation method of the collutory containing antibacterial peptide and sodium copper chlorophllin described in claim 1 or 2, is characterized in that concrete steps are:
(1) first Mentholum, Borneolum Syntheticum and essence are dissolved in the ethanol of volume fraction 95%;
(2) successively antibacterial peptide, sodium copper chlorophllin and gallic acid are dissolved in 20～50 parts of deionized waters, stir;
(3) step (1) is mixed with the solution of preparation in step (2), add deionized water to 100 part, stir, standing, must be containing the collutory of antibacterial peptide and sodium copper chlorophllin after filtration.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410010331.3A CN103751031A (en) | 2014-01-09 | 2014-01-09 | Mouthwash containing antimicrobial peptide and sodium copper chlorophyllin and preparation method of mouthwash |
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| CN201410010331.3A CN103751031A (en) | 2014-01-09 | 2014-01-09 | Mouthwash containing antimicrobial peptide and sodium copper chlorophyllin and preparation method of mouthwash |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106389205A (en) * | 2016-10-31 | 2017-02-15 | 广东科玮生物技术股份有限公司 | Mouthwash with sodium copper chlorophyllin |
| CN111658640A (en) * | 2014-12-24 | 2020-09-15 | 狮王株式会社 | Adhesion bonding functional enhancer |
| CN117106050A (en) * | 2022-12-30 | 2023-11-24 | 好易康生物科技(广州)有限公司 | Antibacterial peptide for nonspecific targeted inhibition of pathogenic bacteria and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1702083A (en) * | 2005-01-14 | 2005-11-30 | 广州华桑生物工程有限公司 | Antibacterial peptide DC and its preparing process and use |
| CN102988192A (en) * | 2012-11-23 | 2013-03-27 | 广州市科玮生物技术有限公司 | Chlorophyll mouth wash and preparation method thereof |
-
2014
- 2014-01-09 CN CN201410010331.3A patent/CN103751031A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1702083A (en) * | 2005-01-14 | 2005-11-30 | 广州华桑生物工程有限公司 | Antibacterial peptide DC and its preparing process and use |
| CN102988192A (en) * | 2012-11-23 | 2013-03-27 | 广州市科玮生物技术有限公司 | Chlorophyll mouth wash and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| NANCY MICKELS ET AL: "Clinical and microbial evaluation of a histatin-containing mouthrinse in humans with experimental gingivitis", 《JOURNAL OF CLINICAL PERIODONTOLOGY》, no. 28, 31 December 2001 (2001-12-31), pages 404 - 410 * |
| 中华人民共和国卫生部药典委员会: "《中华人民共和国卫生部药品标准：维吾尔药分部》", 31 October 1999, 新疆科技卫生出版社, article "西帕依固龈液", pages: 123 * |
| 董世雷等: "抗菌肽及其应用前景概述", 《浙江农业学报》, vol. 23, no. 6, 31 December 2011 (2011-12-31), pages 1274 - 1281 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111658640A (en) * | 2014-12-24 | 2020-09-15 | 狮王株式会社 | Adhesion bonding functional enhancer |
| CN106389205A (en) * | 2016-10-31 | 2017-02-15 | 广东科玮生物技术股份有限公司 | Mouthwash with sodium copper chlorophyllin |
| CN106389205B (en) * | 2016-10-31 | 2017-07-25 | 广东科玮生物技术股份有限公司 | A kind of mouthwash containing sodium copper chlorophyllin |
| CN117106050A (en) * | 2022-12-30 | 2023-11-24 | 好易康生物科技(广州)有限公司 | Antibacterial peptide for nonspecific targeted inhibition of pathogenic bacteria and application thereof |
| CN117106050B (en) * | 2022-12-30 | 2024-02-23 | 好易康生物科技(广州)有限公司 | Antibacterial peptide for nonspecific targeted inhibition of pathogenic bacteria and application thereof |
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Application publication date: 20140430 |