CN105193665A - Blumea balsamifera oral care solution, and preparation method and application thereof - Google Patents
Blumea balsamifera oral care solution, and preparation method and application thereof Download PDFInfo
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- CN105193665A CN105193665A CN201510699785.0A CN201510699785A CN105193665A CN 105193665 A CN105193665 A CN 105193665A CN 201510699785 A CN201510699785 A CN 201510699785A CN 105193665 A CN105193665 A CN 105193665A
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- blumeae balsamiferae
- herba blumeae
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- nursing liquid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
Abstract
The invention discloses a blumea balsamifera oral care solution, and a preparation method and application thereof. The blumea balsamifera oral care solution is prepared from the following components in percentage by weight: 0.03 to 0.25 percent of a blumea balsamifera extract, 0.15 to 0.8 percent of an active ingredient stabilizer, 0.6 to 2 percent of a sweetening agent, 6 to 15 percent of a moisturizing agent, 0.1 to 0.5 percent of an antibacterial agent, 0.03 to 0.1 percent of edible essence, 15 to 25 percent of edible alcohol and 56.36 to 78.09 percent of distilled water, wherein the antibacterial agent is benzoic acid and/or A.SAP. The blumea balsamifera extract for the blumea balsamifera oral care solution contains natural Chinese herbal medicine ingredients, tastes cool and smells fragrant, and the prepared blumea balsamifera oral care solution is clear in appearance and stable in property, has the functions of cleaning an oral cavity, eliminating a peculiar smell, fighting bacteria, eliminating inflammation, clearing heat from a throat and preventing various oral diseases, and has an important application value.
Description
Technical field
The invention belongs to field of oral care, particularly, relate to a kind of Herba Blumeae Balsamiferae oral nursing liquid and its preparation method and application.
Background technology
Oral cavity is gastral first passage, and food enters digestive tract by oral cavity, and oral environment health is very important to body, is the position of the easiest breed bacteria and microorganism.When body defenses function reduction, the pathogenic bacterium in oral cavity can amount reproduction, causes that oral cavity is unclean even occurs oral cavity or lower respiratory infection.Mouth care is by taking certain method, in conjunction with corresponding oral care implement, selects suitable oral nursing liquid, to reach cleaning oral cavity, remove bacterial plaque, prevent and treat oral inflammation, to prevent aspiration pneumonitis and the object improving patients ' life quality.Therefore mouth care is a substance in Clinical Basis nursing manipulative skill, is to keep one of patient's oral cavity cleaning, prophylactic means, and the quality height of mouth care directly has influence on the quality of basic nursing.
At present relative to other oral care product, oral nursing liquid have simultaneously easy to carry, remove halitosis and advantage such as treatment oral disease etc., also the complication that oral disease causes can be removed in time, as the dyssomnia, psychentonia, dyspepsia etc. that cause because of mouth disease.U.S. food and drug administration (FoodandDrugAdministration, FDA) oral nursing liquid of certification has the large class of quintessence oil and biguanide compound etc. two, containing quintessence oil class oral nursing liquid have permeability strong, use after there will not be the advantages such as gingival discoloration, dysbacteriosis, secondary infection, though containing alcoholic ingredient, harmless to oral cavity partial.And current widely used quintessence oil, be mainly derived from the extract at the positions such as the leaf of plant, flower, seed, fruit, they have good volatility, and have unique fragrance.In addition, this kind of oral nursing liquid can better cleaning oral cavity, prevention oral disease, its antibacterial feature is has a broad antifungal spectrum, toxicity is low, anaphylaxis is few, can use in conjunction, on minimizing dental plaque than traditional oral care liquid advantageously, safety is higher, and there is good, the non-irritating feature of mouthfeel, be therefore subject to the favor of more and more consumer.
Herba Blumeae Balsamiferae (
blumeabalsamiferaL.dC.) be Compositae Herba Blumeae Balsamiferae platymiscium, perennial wooden herbaceous plant or undershrub, be used as medicine with root, twig, leaf, originates in Yunnan, Guizhou, Guangxi, Guangdong, Hainan, Fujian and Taiwan, be born in border more, sylvan life, valley floor, riverbed or meadow.Its nature and flavor are pungent, micro-hardship, and tepor has detumescence of dispeling the wind, effect of promoting blood circulation to remove blood stasis, can be used for treatment flu, rheumatic arthritis, puerperal exposure to wind pain, dysmenorrhea; External traumatic is damaged, and furuncle pain is swollen, eczema, the effects such as dermatitis.Main containing volatile oil and flavones ingredient in Herba Blumeae Balsamiferae, there is the pharmacologically active such as antibacterial, antioxidation and anti-inflammatory and antalgic preferably.At present, be that main composition is prepared the oral nursing liquid obtained and be yet there are no report with Herba Blumeae Balsamiferae, need a inhibiting bacteria and diminishing inflammation of research and development, treatment oral ulcer, gingival hemorrhage, periodontitis badly, dispel halitosis, repair oral mucosa and alleviate many effects mouth care liquid product of gingival swelling and pain.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of Herba Blumeae Balsamiferae oral nursing liquid.The raw material of this oral nursing liquid is the extract of plant Herba Blumeae Balsamiferae, this extract fragrant, there is effect of anti-inflammation, reducing swelling and alleviating pain, with Herba Blumeae Balsamiferae extract be main component Herba Blumeae Balsamiferae oral nursing liquid can not only inhibiting bacteria and diminishing inflammation, treat oral ulcer, dispel halitosis, gingival hemorrhage, periodontitis can also be prevented, repair oral mucosa and alleviate gingival swelling and pain etc.
Another object of the present invention is to the preparation method that above-mentioned Herba Blumeae Balsamiferae oral nursing liquid is provided.
Another object of the present invention is to the application that above-mentioned Herba Blumeae Balsamiferae oral nursing liquid is provided.
The present invention is achieved by the following technical programs.
A kind of Herba Blumeae Balsamiferae oral nursing liquid, comprises each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.03 ~ 0.25%
Active component stabilizing agent 0.15 ~ 0.8%
Sweeting agent 0.6 ~ 2%
Wetting agent 6 ~ 15%
Antibacterial 0.1 ~ 0.5%
Edible essence 0.03 ~ 0.1%
Edible ethanol 15 ~ 25%
Distilled water 56.36 ~ 78.09%
Wherein, described antibacterial is benzoic acid and/or A.SAP.
Raw material of the present invention is the extract of plant Herba Blumeae Balsamiferae, because Herba Blumeae Balsamiferae extract is insoluble in water, therefore inventor have selected certain density ethanol as solvent and active component stabilizing agent as chaotropic agent, have passed through a large amount of experimental studies have found that, alcohol concentration is higher, the amount of dissolving the active component stabilizing agent needed for identical Herba Blumeae Balsamiferae extract is less, and screens active component stabilizing agent with this; And the ethanol considering high concentration is on the irritating impact of mouthfeel, and with other components coordinating as sweeting agent, edible essence, wetting agent and antibacterial, final optimization pass obtains the formula of Herba Blumeae Balsamiferae oral nursing liquid.Inventor finds through large quantity research, only have when antibacterial selects benzoic acid and/or A.SAP, itself and Herba Blumeae Balsamiferae extract are arranged in pairs or groups and are obtained anti-inflammation that Herba Blumeae Balsamiferae oral nursing liquid just can reach excellent, reducing swelling and alleviating pain and treat the effect of oral ulcer etc., simultaneously, inventor also optimizes the relative amount of Herba Blumeae Balsamiferae extract and antibacterial, finally obtains the above-mentioned formula of oral protecting liquor of the present invention.
Preferably, described Herba Blumeae Balsamiferae extract be Chinese mugwort powder or Oleum Blumeae Balsamiferae in one or both.
Preferably, described active component stabilizing agent is one or both in Tween 80, polyoxyethylene hydrogenated Oleum Ricini or sodium lauryl sulphate.
Preferably, described antibacterial is A.SAP.
The features such as antibacterial A.SAP is the natural organic anti-bacterial deodorizer using soybean extraction liquid as main composition, has high safety, broad-spectrum high efficacy, vitality is long and antibacterial effect remarkable, colourless, transparent, odorlessness.
Preferably, described sweeting agent is one or both in aspartame, xylitol, cyclamate or acesulfame potassium.
Preferably, described wetting agent is one or both in sorbitol, glycerol or propylene glycol.
Preferably, described edible essence is one or more in vanilla, Green tea essence, Mint Essence or blueberry flavor.
The present invention also provides the preparation method of above-mentioned Herba Blumeae Balsamiferae oral nursing liquid, comprises the steps:
S1. Herba Blumeae Balsamiferae extract is dissolved in edible ethanol and active component stabilizing agent, makes solution clear stable;
S2. sweeting agent, edible essence and wetting agent are joined in S1 gained solution, dissolve mixing;
S3. join in S3 gained solution by antibacterial, distilled water, mixing, filters to obtain Herba Blumeae Balsamiferae oral nursing liquid.
Preferably, namely described S3 obtains Herba Blumeae Balsamiferae mouth care liquid product by after solution mixing by standing, filtration, fill.
The present invention also provides the application of above-mentioned Herba Blumeae Balsamiferae oral nursing liquid suppressing one or more bacterium in staphylococcus aureus, escherichia coli, Candida albicans or Klebsiella Pneumoniae.
Preferably, described Herba Blumeae Balsamiferae oral nursing liquid dilution 1 ~ 10 times during application, its bacteriostasis rate is all greater than 92.1%.
The present invention also provides the application of above-mentioned Herba Blumeae Balsamiferae oral nursing liquid in treatment oral ulcer.
Compared with prior art, beneficial effect of the present invention is: raw material of the present invention is the extract of plant Herba Blumeae Balsamiferae, this extract fragrant, there is effect of anti-inflammation, reducing swelling and alleviating pain, with Herba Blumeae Balsamiferae extract be main component Herba Blumeae Balsamiferae oral nursing liquid can not only inhibiting bacteria and diminishing inflammation, treat oral ulcer, dispel halitosis, gingival hemorrhage, periodontitis can also be prevented, repair oral mucosa and alleviate gingival swelling and pain.
Accompanying drawing explanation
Fig. 1 is the fungistatic effect comparison diagram to staphylococcus aureus in application examples 1 bacteriostatic test.
Fig. 2 is the fungistatic effect comparison diagram to escherichia coli in application examples 1 bacteriostatic test.
Fig. 3 is the fungistatic effect comparison diagram to Candida albicans in application examples 1 bacteriostatic test.
Fig. 4 is the fungistatic effect comparison diagram to Klebsiella Pneumoniae in application examples 1 bacteriostatic test.
Fig. 5 tissue that to be application examples 2 occur without ulcer is through the histopathology slide figure of HE staining under × 20 power microscopes.
Fig. 6 is that application examples 2 tissue of ulcer occurs through the histopathology slide figure of HE staining under × 20 power microscopes.
Fig. 7 be application examples 2 ulcer cure after tissue through the histopathology slide figure of HE staining under × 20 power microscopes.
Fig. 8 is the rat ulcer the average healing block diagram of application examples 2 different disposal group.
Fig. 9 is the comparison diagram of each formula to healing time.
Detailed description of the invention
Below in conjunction with Figure of description and specific embodiment, the present invention is described in further details, but embodiment does not limit in any form the present invention.Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
embodiment 1
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.06%
Active component stabilizing agent 0.19%
Sweeting agent 1.00%
Wetting agent 10.00%
Antibacterial 0.20%
Edible essence 0.04%
Edible ethanol 15.00%
Distilled water 73.51%
Wherein, described Herba Blumeae Balsamiferae extract is Chinese mugwort powder, and described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, and described sweeting agent is xylitol, and described wetting agent is glycerol, and described antibacterial is A.SAP, and described edible essence is Green tea essence.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is as follows:
S1. Herba Blumeae Balsamiferae extract is dissolved in edible ethanol and active component stabilizing agent, makes solution clear stable;
S2. sweeting agent, edible essence and wetting agent are joined in S1 gained solution, dissolve mixing;
S3. join in S3 gained solution by antibacterial, distilled water, mixing, filters to obtain Herba Blumeae Balsamiferae oral nursing liquid.
embodiment 2
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.06%
Active component stabilizing agent 0.19%
Sweeting agent 1.40%
Wetting agent 14.00%
Antibacterial 0.20%
Edible essence 0.05%
Edible ethanol 15.00%
Distilled water 69.10%
Wherein, described Herba Blumeae Balsamiferae extract is Chinese mugwort powder, and described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, and described sweeting agent is xylitol, and described wetting agent is propylene glycol, and described antibacterial is benzoic acid, and described edible essence is blueberry flavor.The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 3
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.12%
Active component stabilizing agent 0.40%
Sweeting agent 1.40%
Wetting agent 14.00%
Antibacterial 0.20%
Edible essence 0.05%
Edible ethanol 20.00%
Distilled water 63.83%
Wherein, described Herba Blumeae Balsamiferae extract is Chinese mugwort powder, and described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, and described sweeting agent is aspartame, and described wetting agent is glycerol, and described antibacterial is A.SAP, and described edible essence is vanilla.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 4
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.03%
Active component stabilizing agent 0.15%
Sweeting agent 0.6%
Wetting agent 6.00%
Antibacterial 0.10%
Edible essence 0.03%
Edible ethanol 15.00%
Distilled water 78.09%
Wherein, described Herba Blumeae Balsamiferae extract is Oleum Blumeae Balsamiferae, and described active component stabilizing agent is sodium lauryl sulphate and Tween 80 (1:1), described sweeting agent is cyclamate, described wetting agent is glycerol, and described antibacterial is A.SAP, and described edible essence is Mint Essence.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 5
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.12%
Active component stabilizing agent 0.40%
Sweeting agent 1.00%
Wetting agent 10.00%
Antibacterial 0.40%
Edible essence 0.05%
Edible ethanol 25.00%
Distilled water 63.03%
Wherein, described Herba Blumeae Balsamiferae extract is Oleum Blumeae Balsamiferae and Chinese mugwort powder (1:1), described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, described sweeting agent is aspartame and cyclamate (2:3), described wetting agent is propylene glycol, described antibacterial is benzoic acid, and described edible essence is blueberry flavor and Mint Essence (3:2).
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 6
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.24%
Active component stabilizing agent 0.76%
Sweeting agent 2.00%
Wetting agent 15.00%
Antibacterial 0.30%
Edible essence 0.06%
Edible ethanol 25.00%
Distilled water 56.64%
Wherein, described Herba Blumeae Balsamiferae extract is Chinese mugwort powder, and described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, described sweeting agent is xylitol and cyclamate (1:1), described wetting agent is sorbitol, and described antibacterial is A.SAP, and described edible essence is Green tea essence.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 7
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.12%
Active component stabilizing agent 0.70%
Sweeting agent 1.50%
Wetting agent 12.00%
Antibacterial 0.20%
Edible essence 0.05%
Edible ethanol 20.00%
Distilled water 65.43%
Wherein, described Herba Blumeae Balsamiferae extract is Oleum Blumeae Balsamiferae, and described active component stabilizing agent is Tween 80, and described sweeting agent is acesulfame potassium, and described wetting agent is sorbitol and propylene glycol (1:2), and described antibacterial is benzoic acid, and described edible essence is vanilla.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 8
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.12%
Active component stabilizing agent 0.60%
Sweeting agent 1.00%
Wetting agent 15.00%
Antibacterial 0.20%
Edible essence 0.05%
Edible ethanol 20.00%
Distilled water 63.03%
Wherein, described Herba Blumeae Balsamiferae extract is Oleum Blumeae Balsamiferae and Chinese mugwort powder (3:4), and described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, described sweeting agent is acesulfame potassium, described wetting agent is propylene glycol, and described antibacterial is A.SAP and benzoic acid (1:1), and described edible essence is blueberry flavor.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 9
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.06%
Active component stabilizing agent 0.60%
Sweeting agent 1.20%
Wetting agent 10.00%
Antibacterial 0.30%
Edible essence 0.05%
Edible ethanol 20.00%
Distilled water 67.79%
Wherein, described Herba Blumeae Balsamiferae extract is Chinese mugwort powder, and described active component stabilizing agent is sodium lauryl sulphate, and described sweeting agent is aspartame, and described wetting agent is glycerol, and described antibacterial is benzoic acid, and described edible essence is blueberry flavor.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
embodiment 10
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.24%
Active component stabilizing agent 0.76%
Sweeting agent 1.50%
Wetting agent 15.00%
Antibacterial 0.20%
Edible essence 0.10%
Edible ethanol 15.00%
Distilled water 67.20%
Wherein, described Herba Blumeae Balsamiferae extract is Chinese mugwort powder, and described active component stabilizing agent is polyoxyethylene hydrogenated Oleum Ricini, described sweeting agent is xylitol and aspartame (2:3), described wetting agent is glycerol and propylene glycol (3:2), and described antibacterial is A.SAP, and described edible essence is Green tea essence.
The preparation method of the Herba Blumeae Balsamiferae oral nursing liquid of above-mentioned formula is identical with embodiment 1.
application examples 1 bacteriostatic experiment
Test liquid selected by the present embodiment bacteriostatic test is the Herba Blumeae Balsamiferae oral nursing liquid (for examination group) in embodiment 1, and contrast liquid is normal saline (matched group).
The bacteriostatic test of described Herba Blumeae Balsamiferae oral nursing liquid is: washed down by the slant culture PBS of staphylococcus aureus, escherichia coli, Candida albicans and Klebsiella Pneumoniae respectively, carry out the preparation (3 × 10 of bacteria suspension
8/ mL) and dilute, obtain 3 × 10
5/ mL bacterium liquid.Get 0.1mL above-mentioned gained bacterium liquid respectively, be added to containing 5mL test liquid in vitro, action time is 2min; Get 0.1mL above-mentioned gained bacterium liquid again, be added to and contrast liquid in vitro containing 5mL, action time is 2min; Get test liquid under the 0.5mL above-mentioned response time respectively and contrast liquid and be added to containing 5mLPBS in vitro, fully mix, be called cessation reaction, obtain test liquid 1 and contrast liquid 1; Get test liquid 1 and each 0.1mL of contrast liquid 1 more respectively, be diluted to 5 times and 10 times with 0.4mL and 0.9mLPBS respectively, be numbered test liquid 2, test liquid 3, contrast liquid 2, contrast liquid 3.Get each 0.5mL of diluent of above-mentioned gained different proportion, in culture dish.(wherein staphylococcus aureus, escherichia coli and Klebsiella Pneumoniae nutrient agar is poured into the cool culture medium 15mL to 40 ~ 45 DEG C, Candida albicans Sabouraud glucose agar is cultivated), rotate culture dish, make it full and uniform, after agar solidification, upset is dull and stereotyped, cultivate 24h (Candida albicans is 48h), do viable bacteria colony counting for 35 ± 2 DEG C.
Fig. 1 ~ 4 are seen for examination group and the matched group comparative result to the fungistatic effect of staphylococcus aureus, escherichia coli, Candida albicans and Klebsiella Pneumoniae in above-mentioned test.To the account form of described Herba Blumeae Balsamiferae oral nursing liquid bacteriostasis rate be: bacteriostasis rate=(control sample clump count-test sample clump count)/control sample clump count.Evaluation criterion is: bacteriostasis rate >=50% ~ 90%, and product has bacteriostasis.
Above-mentioned bacteriostatic test result is as shown in table 1 below, and experimental result shows each strain bacteriostasis rate all >=90%, discloses Herba Blumeae Balsamiferae oral nursing liquid and has good fungistatic effect.
Table 1 Herba Blumeae Balsamiferae oral nursing liquid bacteriostatic test result
application examples 2 oral ulcer effect experiment
The oral ulcer pharmacodynamic experiment of described Herba Blumeae Balsamiferae oral nursing liquid is: get healthy cleaning grade SD rat 21, 7 groups are divided at random after 1 week adaptability is raised and the illumination rhythm and pace of moving things is normal, often organize 3, i.e. normal group, ulcer group, 15% alcohol solvent matched group, JINHOUJIAN PENWUJI positive controls, Herba Blumeae Balsamiferae oral nursing liquid low (0.06%), in (0.12%), (low dose group is the Herba Blumeae Balsamiferae oral nursing liquid in embodiment 1 to high (0.24%) 3 dosage group, middle dosage group is basic identical with embodiment 1 with high dose group component, difference is middle dosage group Herba Blumeae Balsamiferae extract 0.12%, stabilizing agent polyoxyethylene hydrogenated Oleum Ricini 0.40%, distilled water 73.24%, high dose group Herba Blumeae Balsamiferae extract 0.24%, stabilizing agent polyoxyethylene hydrogenated Oleum Ricini 0.76%, distilled water 72.76%).Normal group is left intact, remaining 6 groups of modelings all adopting improvement Chen Qian explicit law to carry out Oral ulcer model, and wherein the not administration of ulcer group allows its normal healing; After all the other each experimental group rat modelings, 24h plays daily 2 times, each 0.6ml, and the spray of solvent control group is with 15% ethanol; Positive controls spray is good for golden larynx; The spray of Herba Blumeae Balsamiferae oral nursing liquid group with Herba Blumeae Balsamiferae oral nursing liquid low (0.6mg/ml), in (1.2mg/ml), high (2.4mg/ml) 3 dose concentrations, observe the time of its healing.
Improvement Chen Qian explicit law modeling: with 10% chloral hydrate (3ml/Kg) through intraperitoneal injection of anesthesia SD rat, get its dorsal position and be fixed on operating-table, upper lower jaw is strutted with mosquito forceps, left and right sides cheek pouch is pulled out gently with flat tooth tweezer, 0.8cm is about in left and right sides cheek pouch tela submucosa with the l0mmol/L methyl viologen solution that No. 5 skin-test needle fan-shaped injection 0.25ml prepare, and with prefabricated diameter be 5mm, to be 100 DEG C of iron nails in methyl viologen injection place touch temperature scalds 3s, namely sees that this place has the white of 5mm to damage; On the right side of 24h perusal, cheek pouch place has diameter to be about the ulcer of 5.5mm, surrounding tissue congestion and edema, and skin surface has yellow pseudomembrane to cover, and has rheuminess thing to ooze out, and in oral cavity, salivation amount increases.
Specimen collection: each group SD rat is put to death and cuts at place of mucosal ulcer, buccal the tissue that 5mm × 2mm reaches Submucosa deeply after last therapeutic administration 24h, the blood of tissue surface is washed away with physiological saline solution, be placed in 4% paraformaldehyde to fix, take out specimen after 24h and carry out paraffin embedding, adopt HE dyeing make histopathologic slide and under light microscopic observation analysis.
Fig. 5 ~ 7 are respectively the light Microscopic observation figure of the tissue after the healing of the tissue occurred without ulcer, the tissue that ulcer occurs and ulcer.
According to grouping situation, the healing time statistics of each rat stomatocace is shown in Fig. 8.Ulcer group started to occur healing in the 8th day, fully recovered when the 10th day; Recovery from illness time 15% alcohol solvent matched group started to occur healing the 9th day in the 7th day; Recovery from illness time JINHOUJIAN PENWUJI positive controls started to occur healing the 6th day in the 5th day; The appearance that starts for the 4th day after viewing of basic, normal, high 3 the dosage groups of Herba Blumeae Balsamiferae oral nursing liquid is healed, and fully recovers when the 7th day.Gold larynx is good for the average healing all short than ulcer group (P<0.05) of the ulcer of positive controls and basic, normal, high group of Herba Blumeae Balsamiferae oral nursing liquid; Healing time between basic, normal, high group of Herba Blumeae Balsamiferae oral nursing liquid compares, no significant difference (P > 0.05).
comparative example 1
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.06%
Active component stabilizing agent 0.19%
Sweeting agent 1.00%
Wetting agent 10.00%
Antibacterial 0.20%
Edible essence 0.04%
Edible ethanol 15.00%
Distilled water 73.51%
Wherein, described antibacterial is thymol, and other are with embodiment 1.
comparative example 2
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.06%
Active component stabilizing agent 0.19%
Sweeting agent 1.00%
Wetting agent 10.00%
Antibacterial 0.20%
Edible essence 0.04%
Edible ethanol 15.00%
Distilled water 73.51%
Wherein, described antibacterial is cinnamic aldehyde, and other are with embodiment 1.
comparative example 3
Present embodiments provide a kind of Herba Blumeae Balsamiferae oral nursing liquid, comprise each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.03%
Active component stabilizing agent 0.15%
Sweeting agent 1.00%
Wetting agent 10.00%
Antibacterial 0.20%
Edible essence 0.04%
Edible ethanol 15.00%
Distilled water 73.58%
Wherein, described antibacterial is tea polyphenols, and other are with embodiment 1.
Bacteriostatic test result under above-mentioned each comparative example formula is as shown in table 2 below.
Table 2 Herba Blumeae Balsamiferae oral nursing liquid fungistatic effect compares
Prescription (low concentration 0.06%) in embodiment 1 and the Herba Blumeae Balsamiferae oral nursing liquid of comparative example 1,2,3 are carried out the oral ulcer effect experiment as described in application examples 2, and its healing time carries out statistical analysis, the results are shown in Figure 9.Wherein embodiment 1 and comparative example 2 have significant difference (P<0.05), not statistically significant (P > 0.05) between all the other prescriptions.Comprehensive the above results is known, and effect of Herba Blumeae Balsamiferae formula of oral protecting liquor anti-inflammation of the present invention, treatment oral ulcer is better than comparative example generally.
Claims (9)
1. a Herba Blumeae Balsamiferae oral nursing liquid, is characterized in that, comprises each component of following percentage by weight:
Herba Blumeae Balsamiferae extract 0.03 ~ 0.25%
Active component stabilizing agent 0.15 ~ 0.8%
Sweeting agent 0.6 ~ 2%
Wetting agent 6 ~ 15%
Antibacterial 0.1 ~ 0.5%
Edible essence 0.03 ~ 0.1%
Edible ethanol 15 ~ 25%
Distilled water 56.36 ~ 78.09%
Wherein, described antibacterial is benzoic acid and/or A.SAP.
2. Herba Blumeae Balsamiferae oral nursing liquid according to claim 1, is characterized in that, described Herba Blumeae Balsamiferae extract be Chinese mugwort powder or Oleum Blumeae Balsamiferae in one or both.
3. Herba Blumeae Balsamiferae oral nursing liquid according to claim 1, is characterized in that, described active component stabilizing agent is one or both in Tween 80, polyoxyethylene hydrogenated Oleum Ricini or sodium lauryl sulphate.
4. Herba Blumeae Balsamiferae oral nursing liquid according to claim 1, is characterized in that, described sweeting agent is one or both in aspartame, xylitol, cyclamate or acesulfame potassium.
5. Herba Blumeae Balsamiferae oral nursing liquid according to claim 1, is characterized in that, described wetting agent is one or both in sorbitol, glycerol or propylene glycol.
6. Herba Blumeae Balsamiferae oral nursing liquid according to claim 1, is characterized in that, described edible essence is one or more in vanilla, Green tea essence, Mint Essence or blueberry flavor.
7. a preparation method for Herba Blumeae Balsamiferae oral nursing liquid described in any one of claim 1 ~ 6, is characterized in that, comprise the steps:
S1. Herba Blumeae Balsamiferae extract is dissolved in edible ethanol and active component stabilizing agent, makes solution clear stable;
S2. sweeting agent, edible essence and wetting agent are joined in S1 gained solution, dissolve mixing;
S3. join in S3 gained solution by antibacterial, distilled water, mixing, filters to obtain Herba Blumeae Balsamiferae oral nursing liquid.
8. Herba Blumeae Balsamiferae oral nursing liquid described in any one of claim 1 ~ 6 is suppressing the application of one or more bacterium in staphylococcus aureus, escherichia coli, Candida albicans or Klebsiella Pneumoniae.
9. the application of Herba Blumeae Balsamiferae oral nursing liquid described in any one of claim 1 ~ 6 in treatment oral ulcer.
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| CN106214716A (en) * | 2016-08-31 | 2016-12-14 | 中国热带农业科学院热带作物品种资源研究所 | A kind of buccal containing Herba Blumeae Balsamiferae extract and preparation method thereof |
| CN106265811A (en) * | 2016-08-31 | 2017-01-04 | 中国热带农业科学院热带作物品种资源研究所 | A kind of oral cavity chewable tablet containing Herba Blumeae Balsamiferae extract |
| CN106491390A (en) * | 2016-12-13 | 2017-03-15 | 美晨集团股份有限公司 | A kind of persistently refrigerant antibacterial oral cavity cleaning and nursing liquid and preparation method thereof |
| CN106913602A (en) * | 2017-01-24 | 2017-07-04 | 中国热带农业科学院热带作物品种资源研究所 | A kind of Blumea balsamifera gynaecologic washing lotion and its preparation method and application |
| CN106983817A (en) * | 2017-05-17 | 2017-07-28 | 海南蛛王药业有限公司 | A kind of great-ventral garden spider slough oral nursing liquid and preparation method thereof |
| CN108836913A (en) * | 2018-08-29 | 2018-11-20 | 海南森祺制药有限公司 | A kind of nauclea officinalis mouthwash and preparation method thereof |
| CN111904903A (en) * | 2020-07-13 | 2020-11-10 | 鲁可 | Oral cavity gargle and preparation method thereof |
| CN112691057A (en) * | 2020-12-23 | 2021-04-23 | 中国热带农业科学院热带作物品种资源研究所 | Compound functional toothpaste and preparation method thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106214716A (en) * | 2016-08-31 | 2016-12-14 | 中国热带农业科学院热带作物品种资源研究所 | A kind of buccal containing Herba Blumeae Balsamiferae extract and preparation method thereof |
| CN106265811A (en) * | 2016-08-31 | 2017-01-04 | 中国热带农业科学院热带作物品种资源研究所 | A kind of oral cavity chewable tablet containing Herba Blumeae Balsamiferae extract |
| CN106491390A (en) * | 2016-12-13 | 2017-03-15 | 美晨集团股份有限公司 | A kind of persistently refrigerant antibacterial oral cavity cleaning and nursing liquid and preparation method thereof |
| CN106913602A (en) * | 2017-01-24 | 2017-07-04 | 中国热带农业科学院热带作物品种资源研究所 | A kind of Blumea balsamifera gynaecologic washing lotion and its preparation method and application |
| CN106983817A (en) * | 2017-05-17 | 2017-07-28 | 海南蛛王药业有限公司 | A kind of great-ventral garden spider slough oral nursing liquid and preparation method thereof |
| CN108836913A (en) * | 2018-08-29 | 2018-11-20 | 海南森祺制药有限公司 | A kind of nauclea officinalis mouthwash and preparation method thereof |
| CN111904903A (en) * | 2020-07-13 | 2020-11-10 | 鲁可 | Oral cavity gargle and preparation method thereof |
| CN111904903B (en) * | 2020-07-13 | 2022-09-20 | 鲁可 | Oral cavity gargle and preparation method thereof |
| CN112691057A (en) * | 2020-12-23 | 2021-04-23 | 中国热带农业科学院热带作物品种资源研究所 | Compound functional toothpaste and preparation method thereof |
| CN113750198A (en) * | 2021-10-21 | 2021-12-07 | 中国热带农业科学院热带作物品种资源研究所 | Compound oral care solution and preparation method thereof |
| CN114569519A (en) * | 2022-04-15 | 2022-06-03 | 贵州黄果树立爽药业有限公司 | Alcohol-free mouth wash and preparation method thereof |
| CN114903869A (en) * | 2022-05-16 | 2022-08-16 | 广州桔子创新科技有限公司 | Nursing liquid blasting bead, production process and intelligent production equipment |
| CN114903869B (en) * | 2022-05-16 | 2023-05-05 | 广州桔子创新科技有限公司 | Bead blasting nursing liquid, production process and intelligent production equipment |
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