CN103750035A

CN103750035A - Method for causing bodies of ornamental fishes to go red by utilizing eggs of ampullaria gigas

Info

Publication number: CN103750035A
Application number: CN201410005238.3A
Authority: CN
Inventors: 胡隐昌; 杨叶欣; 顾党恩; 罗渡; 徐猛; 汪学杰; 牟希东; 宋红梅; 刘超; 刘奕; 罗建仁
Original assignee: Pearl River Fisheries Research Institute CAFS
Current assignee: Pearl River Fisheries Research Institute CAFS
Priority date: 2014-01-03
Filing date: 2014-01-03
Publication date: 2014-04-30

Abstract

The invention discloses a method for causing bodies of ornamental fishes to go red by utilizing eggs of ampullaria gigas. The method is realized by feeding red eggs of ampullaria gigas to ornamental fishes. The method is lasting and stable in coloring effect, simple and convenient in operation and low in cost, achieves the aims of turning harm of the eggs of ampullaria gigas into good and changing wastes into valuable materials, can increase the economic values of the ornamental fishes and has high practical popularization and application significance.

Description

A kind of method that ovum that utilizes Pomacea canaliculata reddens the body colour of pet fish

Technical field

The present invention relates to a kind of biological colorize method, be specifically related to a kind of method that ovum that utilizes Pomacea canaliculata reddens the body colour of pet fish.

Background technology

Pomacea canaliculata ( pomacea canaliculata) be worldwide pernicious invasion aquatic animal, from 1980, introduce existing 30 over-carriages so far, the economic loss of causing to China's agricultural production reaches several hundred million units every year, and 2008 are only Guangdong Province's economic loss just up to 10,000,000,000 yuan, and bio-diversity, the ecosystem on invasion ground are threatened.Meanwhile, Pomacea canaliculata still cause mankind eosinophilic meningoencephalitis Angiostrongylus cantonensis ( angiostrongy luscantonensis) intermediate host, human health is threatened, on China Wenzhou and Beijing and other places, occurred to cause eosinophilic meningoencephalitis colony morbidity event because of edible Pomacea canaliculata.Therefore, Pomacea canaliculata has caused the problems such as serious economy, ecology and human health in China.

China is a lot of to the method for preventing and treating of Pomacea canaliculata harm, mainly comprises cultural control, chemical prevention and biological control.All methods of preventing and treating are all useful when controlling Pomacea canaliculata, and respectively have superiority, but there is limitation, do not have a kind of be overall safety effectively, economical and be suitable for natural ecosystems, therefore, urgently explore the trans-utilization approach of Pomacea canaliculata, both can effectively prevent and treat spiral shell evil, can produce certain economic benefit again, finally realize the best prevention and control strategy that Pomacea canaliculata is turned waste into wealth, turned harm into good.

Pomacea canaliculata meat is all higher containing rich in protein and amino acid, particularly leucine and valine content, similar to fish meal, has using spiral shell meat as fish meal substitute and make the correlative study of local flavor soy sauce.Pomacea canaliculata ovum produces above the water surface, the warning coloration that tool is scarlet, research shows, in ovum, contain abundant ovorubin, slycolipid compounds etc., resist direct sunlight, the external environment unfavorable factor such as dry, the carotenoid of its ovorubin combination 99% is astaxanthin and carboxylate thereof, every about astaxanthin-containing 1.5nmol of ovum.Pomacea canaliculata is the biological invasion of r type countermeasure, and reproductive capacity is extremely strong, and in Guangzhou, a female spiral shell can be laid eggs more than 4300 for 1 year, and therefore, rational exploitation and utilization Pomacea canaliculata ovum, can not only effectively control quantity and the harm of Pomacea canaliculata, and can expand new approaches for enhancing color of pet fish.This respect yet there are no relevant technology report at present.

Summary of the invention

A kind of method that one object of the present invention is to provide ovum that utilizes Pomacea canaliculata that the body colour of pet fish is reddened.

Another object of the present invention is to provide the feed addictive that a kind of body colour that can make fish reddens.

The technical solution used in the present invention is:

The method that the body colour of pet fish is reddened, comprises the throw something and feed ovum of Pomacea canaliculata of pet fish.

Said method specifically comprises the steps:

1) after the ovum of Pomacea canaliculata is pulverized, chorion is separated with ovum liquid;

2) ovum liquid is mixed with basal feed, obtain mixed fodder;

3) every day is to the pet fish step 2 of throwing something and feeding) mixed fodder for preparing.

The ovum of described Pomacea canaliculata refers to red ovum.

Described pet fish is parrot fish.

A biological colouring agent, the ovum that it contains Pomacea canaliculata.

The ovum of described Pomacea canaliculata is red ovum.

A feeding additive aquatic animal, it contains biological colouring agent recited above.

An aquatic feeds, it contains feed addictive recited above.

The invention has the beneficial effects as follows:

The present invention utilizes the ovum of Pomacea canaliculata that the body colour of pet fish is reddened, and coloring effect lasting stability is easy and simple to handle, with low cost, both realized the object that Pomacea canaliculata spiral shell ovum " is turned harm into good, turned waste into wealth ", can increase the economic worth of pet fish again, there is the important actual meaning of applying.

Accompanying drawing explanation

Fig. 1 fish body tail fin a* value is with the variation of Feeding time;

Fig. 2 fish body skin a* value is with the variation of Feeding time;

Fig. 3 parrot fish body weight over time;

Fig. 4 parrot fish body is long over time;

Fig. 5 parrot fish height over time;

Fig. 6 carotenoid content in 60 days parrot fish skin, tail fin and muscle of throwing something and feeding;

Fig. 7 parrot fish coloring effect figure.

The specific embodiment

Below in conjunction with embodiment, the present invention is further illustrated, but be not limited to this.

Embodiment

The present embodiment is added into the ovum of Pomacea canaliculata in basal feed, and it is red that the parrot fish of throwing something and feeding increases its body colour, and concrete steps are as follows:

(1) gather the ovum (spiral shell ovum with redness for well) of Pomacea canaliculata, mud or the weeds of removing stick on top, break into pasty state with grinding or pulverizer;

(2) pour in 50ml centrifuge tube under 4000rpm/min condition centrifugal 10 minutes into;

(3) white foam on pipe upper strata is chosen with spoon, decanted red solution, abandon the precipitation of bottom;

(4) measure the content of carotenoid in ovum liquid, ovum solution be placed on 4 ℃ or-20 ℃ of preservations stand-by;

(5) according to the content of carotenoid in the ovum liquid of measuring, in every kilogram of basal feed, adding respectively the ratio of 100mg, 200mg carotenoid mixes Pomacea canaliculata ovum liquid and stirs with basal feed, for example: in ovum liquid, the content of carotenoid is 80mg/kg, with ovum liquid and the 1kg feed of 1.25kg, stir, obtain the basal feed that carotenoid content is 100mg/kg.Take a small amount of addition manner repeatedly, dry or room temperature is dried for 40 ℃, stand-by;

(6) throw something and feed every day parrot fish 2 times, feeding volume is eaten up and is as the criterion with fish for 5～10 minutes, changes weekly water 2～3 times, continues 60 days;

(7) every 15 days, with electronic balance and ruler, measure the growth correlation proterties such as body weight, body length and height of parrot fish, with Konica Minolta CR-400 color colour difference meter, measure the reading of parrot fish side skin and tail fin;

After (8) 60 days, get skin (containing scale), tail fin and the muscle of parrot fish, measure the content of carotenoid;

After (9) 60 days, continue to throw something and feed 30 days with basal feed, with colour difference meter, measure the reading of parrot fish side skin and tail fin, observe the fish body colour situation of fading.

The parrot fish of the present embodiment test amounts to 180 tails, is divided at random 3 groups, is labeled as respectively A, B, C.60 tails/group, establishes three parallel repetitions for every group, i.e. each parallel 20 tail.Wherein, A group pet fish is thrown something and fed to contain and is equivalent to the mixed fodder that carotenoid content is the Pomacea canaliculata ovum liquid of 100mg; B group pet fish is thrown something and fed to contain and is equivalent to the mixed fodder that carotenoid content is the Pomacea canaliculata ovum liquid of 200mg; C group is the control group of the basal feed of only throwing something and feeding.Every 15 days each parallelly get that 6 tail parrot fishes grow and body colour is measured, after 60 days, each parallelly gets 3 tail parrot fishes, measures the carotenoid content in skin, tail fin and muscle.

Experimental result is as follows:

The a* value scope that colour difference meter is measured is-60～60, changes from small to big and means green going down, red enhancing.From Fig. 1 and Fig. 2, can see, throw something and feed after 60 days, experiment A group parrot fish tail fin a* value increases to 24.33 from-0.85, and the a* value of skin increases to 14.40 from-4.81; B group parrot fish tail fin a* value increases to 29.01 from-0.85, and the a* value of skin increases to 18.26 from-4.81, and all there were significant differences with control group, visible, adds a certain amount of Pomacea canaliculata ovum liquid and can make parrot fish body colour redden in feed, reaches good hyperchromic effect.

Fig. 3, Fig. 4 and Fig. 5 are that the long and height of the body weight, body of fish in the process of throwing something and feeding changes, the difference of testing as seen between A group, B group and control group that there are no significant, and the feed that has shown to add Pomacea canaliculata ovum liquid does not have harmful effect to sashimi (raw fish) length.

Throw something and feed after 60 days, measure the carotenoid content in parrot fish skin, tail fin and muscle, in visible experimental group A group and B group, in the tail fin of fish and skin, the content of carotenoid is all significantly higher than control group, and in muscle, carotenoid content seldom, does not exist significant difference.

Fig. 7 is the throw something and feed coloring effect comparison diagram of 15 days, 30 days, 45 days and 60 days of experiment B group and C group, and visible, after the feed of throwing something and feeding containing Pomacea canaliculata ovum liquid, the body colour of parrot fish obviously reddens, and has improved ornamental value and the economic worth of parrot fish.

Above embodiment is only for introducing preferred case of the present invention, and to those skilled in the art, any apparent changes and improvements of carrying out in the scope that does not deviate from spirit of the present invention, all should be regarded as a part of the present invention.

Claims

1. a method that makes the body colour of pet fish redden, comprises the throw something and feed ovum of Pomacea canaliculata of pet fish.

2. method according to claim 1, is characterized in that, specifically comprises the steps:

2) ovum liquid is mixed with basal feed, obtain mixed fodder;

3. method according to claim 1 and 2, is characterized in that, the ovum of described Pomacea canaliculata refers to red ovum.

4. method according to claim 1 and 2, is characterized in that, described pet fish is parrot fish.

5. a biological colouring agent, the ovum that it contains Pomacea canaliculata.

6. biological colouring agent according to claim 5, is characterized in that, the ovum of described Pomacea canaliculata is red ovum.

7. a feeding additive aquatic animal, it contains the biological colouring agent described in claim 5 or 6.

8. an aquatic feeds, it contains feed addictive claimed in claim 7.