CN103743895B - Detachable glass slide incubator - Google Patents
Detachable glass slide incubator Download PDFInfo
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- CN103743895B CN103743895B CN201410008291.9A CN201410008291A CN103743895B CN 103743895 B CN103743895 B CN 103743895B CN 201410008291 A CN201410008291 A CN 201410008291A CN 103743895 B CN103743895 B CN 103743895B
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- Prior art keywords
- microslide
- frame
- resilient clamp
- incubation
- stainless steel
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- Expired - Fee Related
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- 239000011521 glass Substances 0.000 title abstract 9
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 29
- 239000010935 stainless steel Substances 0.000 claims abstract description 29
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000741 silica gel Substances 0.000 claims abstract description 20
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 20
- 230000002093 peripheral effect Effects 0.000 claims description 9
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 238000011534 incubation Methods 0.000 abstract description 30
- 238000000034 method Methods 0.000 abstract description 29
- 239000007788 liquid Substances 0.000 abstract description 16
- 238000011532 immunohistochemical staining Methods 0.000 abstract description 6
- 230000012447 hatching Effects 0.000 description 23
- 229960001866 silicon dioxide Drugs 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000003364 immunohistochemistry Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000168254 Siro Species 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a detachable glass slide incubator. The detachable glass slide incubator comprises a stainless steel incubation frame, a silica gel cushion layer, and elastic clampers which clamps the stainless steel incubation frame and the silica gel cushion layer on a glass slide, wherein the elastic clampers clamp the incubation frame and the glass slide through clamping openings of the elastic clampers; the incubation frame and the glass slide are clamped together to form a small incubation chamber through clamping parts of the elastic clampers. The detachable glass slide incubator is reasonable in structure; when immunohistochemical staining of a tissue is performed, a non-leakage incubation small chamber is formed by tightly pressing the incubation frame on the glass slide to which the tissue sections adhere by the elastic clampers repeatedly; when anti-body incubation is performed, only a trace amount of anti-body incubation liquid can ensure that all the tissue sections adhered to the glass slide are completely soaked in the anti-body incubation liquid in the process of incubating, and thus the experimental cost for scientific research is saved; other experimental steps can also be prevented from being influenced by the incubation frame by detaching the incubation frame.
Description
Technical field
The present invention relates to a kind of dismountable microslide couveuse.
Background technology
In the research carrying out life science, people often need to use high specificity, highly sensitive immunohistochemistry technology to position histiocytic predetermined substance and quantitatively.The ultimate principle of immunohistochemistry technology first utilizes immunologic known antibodies (first antibody) antigen in tissues observed to be combined.Because the antigen-antibody reaction carried out on tissue and cell is generally sightless, certain label (as enzyme, fluorescein) is attached on first antibody by the method for needs mark, then with the fluorescence that histochemical method shows this label or sends at fluorescent microscope, lazer scan confocal microscope or ultrahigh resolution basis of microscopic observation fluorescein.Accomplish visual for the Cucumber in research object tissue.State in realization mention with mark method certain label (as enzyme, fluorescein) is attached on first antibody time, people often adopt the method having combined the second antibody of certain label and the specific binding of first antibody.
Positioning with time quantitative with immunohistochemistry technology to histiocytic predetermined substance, people often adopt paster decoration method to keep the tandem of the good form of histotomy and clearly each histotomy in experiment.The ultimate principle of paster decoration method is on microtome after Cut tissue sheet, is pasted onto on microslide by this histotomy immediately by section order.The experimental implementation such as what histotomy was follow-up closes, first antibody is hatched, washed, second antibody is hatched, wash, dewater again, transparent and mounting are all carry out under this histotomy is pasted onto the condition of microslide.
The closing of above-mentioned histotomy, wash, wash again, dewater, in transparent and mounting process, because the associated reagents cost used is lower, in addition in washing process, the large usage quantity of cleansing solution.In order to obtain good experiment effect, people often adopt the method by the whole microslide being pasted with histotomy is placed in above-mentioned related liquid to process.But when carrying out first antibody and second antibody is hatched, because antibody price is higher, (price of monoclonal primary antibody 100 μ l general is at present about 3000 yuan, the price of special monoclonal primary antibody can be higher), and when using, attenuable multiple again can not be very large.Therefore, antibody especially first antibody be the major part of whole experimental cost.So positioning with time quantitative with immunohistochemistry technology to histiocytic predetermined substance, the method by the whole microslide being pasted with histotomy is placed in antibody incubation liquid can not be adopted.
When carrying out immunohistochemical staining with paster method, people adopt the method on histotomy antibody incubation liquid being directly added in microslide adhesion usually.But when using this method, antibody incubation liquid has been added and can not preserved and diffuse out microslide loss, adding the antibody incubation liquid lacked on histotomy can, in the process of hatching, can not keep the histotomy on microslide to be immersed in all the time in antibody incubation liquid because of moisture evaporation in Incubating Solution.Sometimes, people also can be used in the method for to draw a frame around histotomy that microslide attaches with oily material, and add antibody incubation liquid in this oiliness picture frame, prevent the spilling of antibody incubation liquid.But although the method that the method is directly added in than by antibody incubation liquid on the histotomy of microslide attaching improves to some extent, but use the method, due to the poor ability that oiliness picture frame stops antibody incubation liquid excessive, can not ensure that histotomy is immersed in antibody incubation liquid all the time in whole antibody incubation process.And in antibody incubation, washing, dehydration, clearing process, these oily materials can come off and lose the effect stoping the antibody incubation liquid in antibody incubation process excessive; And oily material can occur come off and adhere on tissue sections, affect the situation that histotomy is observed; When oily material also can dissolve in relevant experiment reagent in histotomy process, the recycling of experiment reagent affected because polluting experiment reagent and the treatment effect of histotomy.
Summary of the invention
The object of the present invention is to provide a kind of rational in infrastructure, all experimental procedure energy convenience and high-efficiencies carry out, and can obtain dismountable microslide couveuse of the experimental result of good immunohistochemical staining.
Technical solution of the present invention is:
A kind of dismountable microslide couveuse, it is characterized in that: comprise stainless steel and hatch frame, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Described silica gel bed course has hatches the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide; The peripheral dimension that stainless steel hatches the peripheral dimension of frame and silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; Described resilient clamp comprises resilient clamp root, the clamping part of resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and provides the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide; Being clamped in by resilient clamp by the clamp port of resilient clamp hatches on frame and microslide, and the clamping part of resilient clamp will be hatched the formation that is clamped together of frame and microslide and be hatched cell.
Its using method is: stainless steel is hatched frame and silicagel pad and be laminated to and be pasted with on the microslide of histotomy, guarantees that all histotomies are all hatched bottom cell what hatch that frame and microslide formed; Stainless steel is selected to hatch frame and any one side in microslide up and down overlapping four limits, hand-held resilient clamp root, the clamp port of resilient clamp is aimed at the structure of hatching frame and microslide and being stacked up and down, resilient clamp is pushed the structure of hatching frame and microslide and being stacked up and down, ensure that the clamping part of resilient clamp will be hatched together with frame is pressed on microslide, its excess-three limit is repeated above-mentioned action and is sandwiched by resilient clamp, guarantee to hatch frame and microslide compact siro spinning technology, what formation was not leaked hatches cell; Adding first antibody Incubating Solution to hatching in cell, ensureing be immersed in first antibody Incubating Solution hatching the histotomy bottom cell, carrying out hatching of histotomy on request; First time takes out containing special first antibody Incubating Solution after having hatched, and unloads the resilient clamp of hatching frame and microslide phase stack structure four limit up and down, carries out carrying out washing treatment from hatching frame under microslide is dismantled to the histotomy be pasted onto on microslide; Carry out second antibody when hatching, repeat above-mentioned action.
The present invention is rational in infrastructure, when carrying out the immunohistochemical staining organized, by repeatedly using resilient clamp to be pasted with on the microslide of histotomy by hatching frame being pressed in tightly, formed one do not leak hatch cell.When carrying out antibody incubation, only using the antibody incubation liquid of trace, just ensure that and being pasted onto all histotomies on microslide in the process of hatching, being fully immersed in antibody incubation liquid, having saved the experimental cost of scientific research.Meanwhile, hatching frame by repeatedly unloading, in turn ensure that microslide pre-treatment, organize paster, washing, dehydration, the experimental procedure such as transparent, mounting by the impact of hatching frame, and can not avoid the shortcoming with oily material picture frame around histotomy.All experimental procedure energy convenience and high-efficiencies are carried out, guarantees finally to obtain the experimental result of a good immunohistochemical staining.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
Fig. 1 be dismountable microslide couveuse hatch frame schematic perspective view.
Fig. 2 is the microslide schematic perspective view of dismountable microslide couveuse.
Fig. 3 is the resilient clamp schematic perspective view of dismountable microslide couveuse.
Fig. 4 is dismountable microslide couveuse longitudinal profile schematic diagram.
In figure: 1. stainless steel hatches frame: be pressed on microslide, be that bottom surface is formed and hatches cell with microslide by the coffin of centre jointly with the silica gel bed course below it.
2. stainless steel hatches the silica gel bed course of frame: have and hatch the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide.
3. microslide: carrying histotomy, and as hatching the bottom surface of cell.
4. resilient clamp root: connect resilient clamp clamping part, and the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide is provided.
5. the clamping part of resilient clamp: the formation that is clamped together of frame and microslide will be hatched and hatch cell.
6. the clamp port of resilient clamp: by this clamp port resilient clamp is clamped in and hatches on frame and microslide.
7. hatch cell: form small space and deposit Incubating Solution, accomplish the Incubating Solution both using trace, in turn ensure that the histotomy on microslide is immersed in Incubating Solution all the time.
8. resilient clamp.
Embodiment
A kind of dismountable microslide couveuse, it is characterized in that: comprise stainless steel and hatch frame, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Described silica gel bed course has hatches the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide; The peripheral dimension that stainless steel hatches the peripheral dimension of frame and silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; Described resilient clamp comprises resilient clamp root, the clamping part of resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and provides the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide; Being clamped in by resilient clamp by the clamp port of resilient clamp hatches on frame and microslide, and the clamping part of resilient clamp will be hatched the formation that is clamped together of frame and microslide and be hatched cell.
Its using method is: process the microslide selected, to ensure that histotomy has good sticking effect on microslide according to immunohistochemical requirement.Choose the required tissue of experiment to cut into slices, and be attached on microslide.The histotomy be attached on microslide is carried out the Seal treatment before antibody incubation.After completing above-mentioned experimental procedure, stainless steel is hatched frame (1) and silica gel bed course (2) be pressed in be pasted with histotomy microslide (3) on, guarantee that all histotomies all hatch cell (7) bottom what hatch that frame and microslide formed.Select to hatch frame and any one side in microslide up and down overlapping four limits, hand-held resilient clamp root (4), the clamp port (6) of resilient clamp is aimed at the structure of hatching frame and microslide and being stacked up and down, resilient clamp is pushed the structure of hatching frame and microslide and being stacked up and down.Ensure that the clamping part (5) of resilient clamp will hatch frame and microslide forcing together tightly.Its excess-three limit is repeated above-mentioned action and is sandwiched by resilient clamp (8).Guarantee to hatch frame and microslide close contact, what formation was not leaked hatches cell.To hatching in cell the first antibody Incubating Solution added containing special, ensure that being pasted onto the histotomy of hatching bottom cell can be immersed in containing in special first antibody Incubating Solution.Carry out hatching of histotomy on request.First time takes out containing special first antibody Incubating Solution after having hatched, and unload the resilient clamp of hatching frame and microslide phase stack structure four limit up and down, under microslide dismounting, stainless steel hatches frame and silica gel bed course.Routinely carrying out washing treatment is carried out to the histotomy be pasted onto on microslide.Need to carry out second antibody when hatching, repeat above-mentioned action, again formed do not leak hatch cell, add containing special second antibody Incubating Solution, histotomy hatched.After having hatched, remove containing special second antibody Incubating Solution, the lower stainless steel of dismounting hatches frame and silica gel bed course, washs, dewaters, the aftertreatment such as transparent, mounting to the histotomy be pasted onto on microslide.After all process complete, examine under a microscope the immunohistochemical staining result of histotomy.
Use dismountable microslide couveuse of the present invention, first do not install and hatch frame, convenient is microslide paster effect and the pre-service carried out, and ensures when carrying out histotomy, whole slide surface is smooth, the good paster of histotomy after cutting and histotomy sealing effect.When carrying out first antibody and hatching, microslide is installed and hatches frame, is formed one do not leak hatch cell, use micro-first antibody Incubating Solution, all histotomies be pasted onto on microslide just can be made to be fully immersed in first antibody Incubating Solution.When first antibody has been hatched, after microslide is pulled down and hatched frame, the histotomy be pasted onto on microslide is fully washed.Wash and carried out second antibody when hatching, microslide is installed again and hatches frame, what formation one was not leaked again hatches cell, and the same second antibody Incubating Solution using trace, just can make all histotomies be pasted onto on microslide be fully immersed in second antibody Incubating Solution.When second antibody has been hatched, again from after microslide is pulled down and hatched frame, carry out again washing fully to the histotomy be pasted onto on microslide, dewater, transparent and mounting process.To obtain good immunohistochemistry results.
Claims (1)
1. a dismountable microslide couveuse, is characterized in that: comprise stainless steel and hatch frame, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Described silica gel bed course has hatches the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide; The peripheral dimension that stainless steel hatches the peripheral dimension of frame and silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; Described resilient clamp comprises resilient clamp root, the clamping part of resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and provides the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide; Being clamped in by resilient clamp by the clamp port of resilient clamp hatches on frame and microslide, and the clamping part of resilient clamp will be hatched the formation that is clamped together of frame and microslide and be hatched cell.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410008291.9A CN103743895B (en) | 2014-01-09 | 2014-01-09 | Detachable glass slide incubator |
| CN201510091592.7A CN104614514B (en) | 2014-01-09 | 2014-01-09 | Dismountable microslide couveuse that experimental procedure energy convenience and high-efficiency carries out |
| CN201510091595.0A CN104614517B (en) | 2014-01-09 | 2014-01-09 | The using method of dismountable microslide couveuse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410008291.9A CN103743895B (en) | 2014-01-09 | 2014-01-09 | Detachable glass slide incubator |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510091595.0A Division CN104614517B (en) | 2014-01-09 | 2014-01-09 | The using method of dismountable microslide couveuse |
| CN201510091592.7A Division CN104614514B (en) | 2014-01-09 | 2014-01-09 | Dismountable microslide couveuse that experimental procedure energy convenience and high-efficiency carries out |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103743895A CN103743895A (en) | 2014-04-23 |
| CN103743895B true CN103743895B (en) | 2015-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410008291.9A Expired - Fee Related CN103743895B (en) | 2014-01-09 | 2014-01-09 | Detachable glass slide incubator |
| CN201510091595.0A Expired - Fee Related CN104614517B (en) | 2014-01-09 | 2014-01-09 | The using method of dismountable microslide couveuse |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510091595.0A Expired - Fee Related CN104614517B (en) | 2014-01-09 | 2014-01-09 | The using method of dismountable microslide couveuse |
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| CN (2) | CN103743895B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104614514B (en) * | 2014-01-09 | 2016-04-20 | 南通大学 | Dismountable microslide couveuse that experimental procedure energy convenience and high-efficiency carries out |
| WO2021068560A1 (en) * | 2020-06-28 | 2021-04-15 | 南通大学 | Convenient-assembly, detachable incubator for microscope slide |
| WO2021073317A1 (en) * | 2020-09-09 | 2021-04-22 | 南通大学 | Method for using anti-evaporation detachable-type slide incubator |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9924222D0 (en) * | 1999-10-14 | 1999-12-15 | Imp College Innovations Ltd | Assay device |
| US6569674B1 (en) * | 1999-12-15 | 2003-05-27 | Amersham Biosciences Ab | Method and apparatus for performing biological reactions on a substrate surface |
| EP1259324A2 (en) * | 2000-02-18 | 2002-11-27 | Aclara BioSciences, Inc. | Multiple-site reaction device and method |
| US7371348B2 (en) * | 2002-06-14 | 2008-05-13 | Agilent Technologies | Multiple array format |
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| US7341841B2 (en) * | 2003-07-12 | 2008-03-11 | Accelr8 Technology Corporation | Rapid microbial detection and antimicrobial susceptibility testing |
| CN1289904C (en) * | 2003-08-01 | 2006-12-13 | 博奥生物有限公司 | Micro array reaction unit and its use |
| JP3860174B2 (en) * | 2004-03-01 | 2006-12-20 | 倉敷紡績株式会社 | Simultaneous multi-sample hybridization method |
| US20070056898A1 (en) * | 2004-05-13 | 2007-03-15 | Keith Wesner | Ablated predetermined surface geometric shaped boundary formed on porous material mounted on a substrate and methods of making same |
| CN101314755B (en) * | 2007-05-30 | 2012-05-30 | 中山大学达安基因股份有限公司 | Partioning hybridization reaction device for biological chip |
| CN201607568U (en) * | 2009-12-17 | 2010-10-13 | 天津市林业果树研究所 | Gasket for silica gel cover glass |
| CN202066732U (en) * | 2011-06-03 | 2011-12-07 | 天津市宝坻区人民医院 | Constant-temperature incubation device for immunohistochemistry |
| CN102899248B (en) * | 2012-10-25 | 2014-11-26 | 杭州奥盛仪器有限公司 | Humidification temperature-controlled oscillation type hybridization device |
| CN203144386U (en) * | 2013-03-31 | 2013-08-21 | 王宏江 | Novel sperm detection plate |
| CN203350255U (en) * | 2013-07-02 | 2013-12-18 | 武汉三鹰生物技术有限公司 | Adjustable horizontal incubation box |
-
2014
- 2014-01-09 CN CN201410008291.9A patent/CN103743895B/en not_active Expired - Fee Related
- 2014-01-09 CN CN201510091595.0A patent/CN104614517B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103743895A (en) | 2014-04-23 |
| CN104614517B (en) | 2016-03-02 |
| CN104614517A (en) | 2015-05-13 |
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