CN104698163B - The using method of the detachable also microslide couveuse of volume adjustable - Google Patents
The using method of the detachable also microslide couveuse of volume adjustable Download PDFInfo
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- CN104698163B CN104698163B CN201510052601.1A CN201510052601A CN104698163B CN 104698163 B CN104698163 B CN 104698163B CN 201510052601 A CN201510052601 A CN 201510052601A CN 104698163 B CN104698163 B CN 104698163B
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- 238000000034 method Methods 0.000 title claims description 32
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 31
- 239000010935 stainless steel Substances 0.000 claims abstract description 31
- 238000011534 incubation Methods 0.000 claims abstract description 24
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000741 silica gel Substances 0.000 claims abstract description 22
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 22
- 241000397426 Centroberyx lineatus Species 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 8
- 230000012447 hatching Effects 0.000 claims description 42
- 238000002474 experimental method Methods 0.000 claims description 9
- 230000002093 peripheral effect Effects 0.000 claims description 9
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 238000011532 immunohistochemical staining Methods 0.000 claims description 6
- 238000013508 migration Methods 0.000 claims description 6
- 230000005012 migration Effects 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 230000002055 immunohistochemical effect Effects 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 15
- 238000011160 research Methods 0.000 abstract description 3
- 229960001866 silicon dioxide Drugs 0.000 description 14
- 238000003364 immunohistochemistry Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Urology & Nephrology (AREA)
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- Medicinal Chemistry (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of detachable and microslide couveuse of volume adjustable, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; The inwall four sides that stainless steel hatches frame and rubber cushion layer arranges a dovetail groove respectively, and dovetail groove coordinates with the camera incubata Space adjustment block grafting with swallow-tail form convex body.The present invention is rational in infrastructure, ensure that when carrying out antibody incubation, use more micro-antibody incubation liquid, just obtain all histotomies be pasted onto on microslide and can be fully immersed in effect in antibody incubation liquid, further save the experimental cost of scientific research.
Description
The application is application number: the divisional application of 201410012882.3, the applying date: 2014.1.13, title " the microslide couveuse of detachable also volume adjustable ".
Technical field
The present invention relates to a kind of microslide couveuse of detachable also volume adjustable.
Background technology
In the research carrying out life science, people often need to use high specificity, highly sensitive immunohistochemistry technology to position histiocytic predetermined substance and quantitatively.The ultimate principle of immunohistochemistry technology first utilizes immunologic known antibodies (first antibody) to need to identify that antigen is combined in observed tissue.Association reaction due to the antigen-antibody carried out on histocyte is generally sightless, need certain label (as enzyme, fluorescein) to be connected on first antibody, then with the fluorescence that histochemical method shows this label or sends at fluorescent microscope, lazer scan confocal microscope or ultrahigh resolution basis of microscopic observation fluorescein by the method for mark again.Make the Cucumber in observed histocyte visual.State in realization mention with mark method certain label (as enzyme, fluorescein) is attached on first antibody time, people often adopt the method having combined the second antibody of certain label and the specific binding of first antibody.
Positioning with time quantitative with immunohistochemistry technology to histiocytic predetermined substance, the method that people adopt paster to dye usually keeps the experiment good form of histotomy and the tandem of clearly each histotomy.The ultimate principle of the method for paster dyeing is on microtome after Cut tissue sheet, is pasted onto on microslide by this histotomy immediately by section order.The closing of follow-up histotomy, first antibody is hatched, is washed, second antibody is hatched, wash, dewater again, transparent and mounting is all carry out be pasted onto the condition on microslide at this histotomy under.
The closing of above-mentioned histotomy, wash, wash again, dewater, in transparent and mounting process, because the reagent cost preparing these solution is lower, in addition in washing process, the large usage quantity of cleansing solution.In order to obtain good experiment effect, the whole microslide being pasted with histotomy is placed in above-mentioned related solution and processes by people usually.But when carrying out first antibody and second antibody is hatched, because antibody price is higher, (price of monoclonal primary antibody 100 μ l general is at present 3000 yuan, special monoclonal first antibody price can be higher), and when using, attenuable multiple again can not be very large.Therefore, antibody especially first antibody become the major part of immunohistochemical assay cost.So positioning with time quantitative with immunohistochemistry technology to histiocytic predetermined substance, can not come by the method that the whole microslide being pasted with histotomy is placed in antibody incubation liquid.
People are when paster method carries out immunohistochemical staining, and antibody incubation liquid is directly added in the method on the histotomy of microslide by employing usually.But when using this method, antibody incubation liquid has been added and can not preserved and diffuse out microslide loss, adding the antibody incubation liquid lacked on histotomy can, in the process of hatching, can not keep the histotomy on microslide to be immersed in all the time in antibody incubation liquid because of moisture evaporation in Incubating Solution.Sometimes, people also can be used in the method for to draw a frame around histotomy that microslide attaches with oily material, and add antibody incubation liquid in this oiliness picture frame, prevent the spilling of antibody incubation liquid.But though the method that the method is directly added in than by antibody incubation liquid on the histotomy of microslide attaching has improvements, but use the method, due to the poor ability that oiliness picture frame stops antibody incubation liquid excessive, can not ensure that histotomy is immersed in antibody incubation liquid all the time in whole antibody incubation process.And in antibody incubation, washing, dehydration, clearing process, these oily materials can lose because coming off and stop the effect that in antibody incubation process, antibody incubation liquid is excessive; When oily material occurring and coming off and adhere on tissue sections, final sections observation effect can be affected; And oily material, also can be molten in relevant experimental solutions in the processing procedure of histotomy, pollute experimental solutions and the recycling of experimental solutions that affects and the treatment effect of histotomy.
Summary of the invention
The object of the present invention is to provide a kind of rational in infrastructure, all experimental procedure energy convenience and high-efficiencies carry out, and can obtain the detachable of good immunohistochemical staining experimental result and the microslide couveuse of volume adjustable.
Technical solution of the present invention is:
A detachable and microslide couveuse for volume adjustable, it is characterized in that: comprise stainless steel and hatch frame, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Described silica gel bed course has hatches the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide; The peripheral dimension that stainless steel hatches the peripheral dimension of frame and silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; Described resilient clamp comprises resilient clamp root, the clamping part of resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and provides the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide; Being clamped in by resilient clamp by the clamp port of resilient clamp hatches on frame and microslide, and the clamping part of resilient clamp will be hatched the formation that is clamped together of frame and microslide and be hatched cell; The inwall four sides that stainless steel hatches frame and silica gel bed course arranges a dovetail groove respectively, dovetail groove coordinates with the camera incubata Space adjustment block grafting with swallow-tail form convex body, regulate the size in camera incubata space, prevent camera incubata Space adjustment block from hatching little indoor migration.
Using method is: stainless steel is hatched frame and silicagel pad and be laminated to and be pasted with on the microslide of histotomy, guarantees the bottom of hatching cell that all histotomies are all being hatched frame and microslide and formed; Select to hatch frame and any one side in microslide up and down overlapping four limits, hand-held resilient clamp root, the clamp port of resilient clamp is aimed at the structure of hatching frame and microslide and being stacked up and down, resilient clamp is pushed the structure of hatching frame and microslide and being stacked up and down.Ensure that the clamping part of resilient clamp will hatch frame and microslide forcing together tightly, its excess-three limit is repeated above-mentioned action and is sandwiched by resilient clamp, guarantees to hatch frame and microslide close contact, and what formation was not leaked hatches cell; Formed do not leak hatch cell after, according to the position that histotomy attaches on microslide, optionally in 4 dovetail grooves of hatching in frame in rectangular wall, insert 1 to 4 camera incubata Space adjustment block, hatch rectangle in frame arbitrarily or the size in polygon space with flexible; After regulating the size in camera incubata space, to hatching in cell the Incubating Solution adding first antibody, ensure that being pasted onto the histotomy of hatching bottom cell can be immersed in the Incubating Solution containing special first antibody; Carry out hatching of histotomy on request, after first antibody has been hatched, the Incubating Solution of sucking-off first antibody, take out the camera incubata Space adjustment block inserting and hatch in frame in rectangular wall dovetail groove, unload the resilient clamp of hatching structure four limit that frame and microslide are stacked up and down, unload from microslide and hatch frame, carrying out washing treatment is carried out to the histotomy be pasted onto on microslide; Need to carry out second antibody when hatching, repeat aforesaid operations.
The present invention is rational in infrastructure, when carrying out the immunohistochemical staining organized, by repeatedly using resilient clamp to be pasted with on the microslide of histotomy by hatching frame being pressed in tightly, formed one do not leak hatch cell.And the size in any one side, flexible antibody incubation room or polygon space is carried out by the method inserting insertion 1 to 4 camera incubata Space adjustment block to antibody incubation room, remove space unnecessary in camera incubata, formed one hatch space suitable do not leak hatch cell.Ensure that when carrying out antibody incubation, using more micro-antibody incubation liquid, just obtain all histotomies be pasted onto on microslide and can be fully immersed in effect in antibody incubation liquid, further save the experimental cost of scientific research.Meanwhile, by repeatedly taking out camera incubata Space adjustment block and unloading and hatch frame, in turn ensure that microslide pre-treatment, organize paster, washing, dehydration, the experimental procedure such as transparent, mounting can not by the impact of hatching frame.All experimental procedure energy convenience and high-efficiencies are carried out, and finally can obtain the experimental result of a good immunohistochemical staining.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
Fig. 1 be detachable and the microslide couveuse of volume adjustable hatch frame schematic perspective view.
Fig. 2 is microslide schematic perspective view that is detachable and the microslide couveuse of volume adjustable.
Fig. 3 is resilient clamp schematic perspective view that is detachable and the microslide couveuse of volume adjustable.
Fig. 4 is camera incubata Space adjustment block schematic perspective view that is detachable and the microslide couveuse of volume adjustable.
Fig. 5 is detachable and the microslide couveuse longitudinal profile schematic diagram of volume adjustable.
In figure:
1. stainless steel hatches frame: be pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide.
2. stainless steel hatches the silica gel bed course of frame: have and hatch the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, is formed when hatching cell, contacts, prevent the leakage of Incubating Solution with microslide.
3. hatch the dovetail groove in frame in rectangular wall: fixing camera incubata Space adjustment block, prevents camera incubata Space adjustment block from hatching little indoor migration.
4. microslide: carrying histotomy, and as hatching the bottom surface of cell.
5. resilient clamp root: connect resilient clamp clamping part, and the handgrip part of resilient clamp when forming microslide couveuse and dismounting microslide couveuse is provided.
The clamping part of 6 resilient clamps: the formation that forces together of frame and microslide will be hatched and hatch cell.
7. the clamp port of resilient clamp: by this clamp port resilient clamp is clamped in and hatches on frame and microslide.
8. the Space adjustment part of camera incubata Space adjustment block: put into and hatch cell, regulates the size in camera incubata space.
9. the swallow-tail form convex body of camera incubata Space adjustment block: insert in the dovetail groove of hatching on frame inwall, prevent camera incubata Space adjustment block from hatching little indoor migration.
10. hatch cell: form small space and deposit Incubating Solution, accomplish the Incubating Solution both using trace, in turn ensure that the histotomy on microslide is immersed in Incubating Solution all the time.
11. resilient clamps.
Embodiment
A detachable and microslide couveuse for volume adjustable, comprise stainless steel and hatch frame, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Described silica gel bed course has hatches the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide; The peripheral dimension that stainless steel hatches the peripheral dimension of frame and silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; Described resilient clamp comprises resilient clamp root, the clamping part of resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and provides the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide; Being clamped in by resilient clamp by the clamp port of resilient clamp hatches on frame and microslide, and the clamping part of resilient clamp will be hatched the formation that is clamped together of frame and microslide and be hatched cell; The inwall four sides that stainless steel hatches frame and silica gel bed course arranges a dovetail groove respectively, dovetail groove coordinates with the camera incubata Space adjustment block grafting with swallow-tail form convex body, regulate the size in camera incubata space, prevent camera incubata Space adjustment block from hatching little indoor migration.
The microslide selected is processed, to ensure that histotomy has good sticking effect on microslide according to immunohistochemical requirement.Choose the required tissue of experiment to cut into slices, and be attached on microslide.The histotomy be attached on microslide is carried out the Seal treatment before antibody incubation.After completing above-mentioned experimental procedure, stainless steel is hatched frame (1) and silica gel bed course (2) and be pressed in and be pasted with (4) on the microslide of histotomy, guarantee the bottom of hatching cell (10) that all histotomies are all being hatched frame and microslide and formed.Select to hatch frame and any one side in microslide up and down overlapping four limits, hand-held resilient clamp root (5), the clamp port (7) of resilient clamp is aimed at the structure of hatching frame and microslide and being stacked up and down, resilient clamp is pushed the structure of hatching frame and microslide and being stacked up and down.Ensure that the clamping part (6) of resilient clamp will hatch frame and microslide forcing together tightly.Its excess-three limit is repeated above-mentioned action and is sandwiched by resilient clamp (11).Guarantee to hatch frame and microslide close contact, what formation was not leaked hatches cell.Formed do not leak hatch cell after, according to the position that histotomy attaches on microslide, optionally in 4 dovetail grooves of hatching in frame in rectangular wall, insert 1 to 4 camera incubata Space adjustment block, camera incubata Space adjustment block is made up of the Space adjustment part (8) of camera incubata Space adjustment block and swallow-tail form convex body (9), hatch rectangle in frame arbitrarily or the size in polygon space on one side with flexible, the camera incubata Space adjustment block of insertion is attached at histotomy bottom camera incubata for standard not hide.After regulating the size in camera incubata space, to hatching in cell the Incubating Solution added containing special first antibody, be sure oing to be pasted onto the histotomy of hatching bottom cell and can be immersed in the Incubating Solution containing special first antibody.Carry out hatching of histotomy on request.After first antibody has been hatched, sucking-off is containing the Incubating Solution of special first antibody, take out and insert the camera incubata Space adjustment block of hatching in frame in rectangular wall dovetail groove, unload the resilient clamp of hatching structure four limit that frame and microslide are stacked up and down, unload from microslide and hatch frame.Routinely carrying out washing treatment is carried out to the histotomy be pasted onto on microslide.Need to carry out second antibody when hatching, repeat aforesaid operations, again formed camera incubata space size suitable do not leak hatch cell, add the Incubating Solution containing special second antibody, histotomy hatched.After having hatched, remove containing special second antibody Incubating Solution.Frame is hatched under repeating aforesaid operations dismounting.The histotomy be pasted onto on microslide is washed, dewaters, the aftertreatment such as transparent, mounting.After all process complete, examine under a microscope the immunohistochemical staining result of histotomy.
Claims (3)
1. the using method of the detachable also microslide couveuse of volume adjustable, it is characterized in that: the described detachable also microslide couveuse of volume adjustable comprises stainless steel and hatches frame, stainless steel is hatched frame and is pressed on microslide, is that bottom surface is formed and hatches cell jointly with the silica gel bed course below it by the coffin of centre with microslide; Described silica gel bed course has hatches the identical intermediate rectangular space of frame with stainless steel, and sticks to stainless steel and hatch on frame, contacts, prevent the leakage of Incubating Solution with microslide; The peripheral dimension that stainless steel hatches the peripheral dimension of frame and silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched frame and silica gel bed course and be clamped in resilient clamp on microslide; Described resilient clamp comprises resilient clamp root, the clamping part of resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and provides the hand position of resilient clamp when forming microslide couveuse and the couveuse operation of dismounting microslide; Being clamped in by resilient clamp by the clamp port of resilient clamp hatches on frame and microslide, and the clamping part of resilient clamp will be hatched the formation that is clamped together of frame and microslide and be hatched cell; The inwall four sides that stainless steel hatches frame and silica gel bed course arranges a dovetail groove respectively, dovetail groove coordinates with the camera incubata Space adjustment block grafting with swallow-tail form convex body, regulate the size in camera incubata space, prevent camera incubata Space adjustment block from hatching little indoor migration;
Using method is: process the microslide selected, to ensure that histotomy has good sticking effect on microslide according to immunohistochemical requirement; Choose the required tissue of experiment to cut into slices, and be attached on microslide; The histotomy be attached on microslide is carried out the Seal treatment before antibody incubation; After completing above-mentioned experimental procedure, stainless steel is hatched frame (1) and silica gel bed course (2) to be pressed in and to be pasted with (4) on the microslide of histotomy, guarantee the bottom of hatching cell (10) that all histotomies are all being hatched frame and microslide and formed; Select to hatch frame and any one side in microslide up and down overlapping four limits, hand-held resilient clamp root (5), the clamp port (7) of resilient clamp is aimed at the structure of hatching frame and microslide and being stacked up and down, resilient clamp is pushed the structure of hatching frame and microslide and being stacked up and down, ensure that the clamping part (6) of resilient clamp will hatch frame and microslide forces together tightly, its excess-three limit is repeated above-mentioned action and is sandwiched by resilient clamp (11), guarantee to hatch frame and microslide close contact, what formation was not leaked hatches cell; Formed do not leak hatch cell after, according to the position that histotomy attaches on microslide, optionally in 4 dovetail grooves of hatching in frame in rectangular wall, insert 1 to 4 camera incubata Space adjustment block, camera incubata Space adjustment block is made up of the Space adjustment part (8) of camera incubata Space adjustment block and swallow-tail form convex body (9), hatch rectangle in frame arbitrarily or the size in polygon space on one side with flexible, the camera incubata Space adjustment block of insertion is attached at histotomy bottom camera incubata for standard not hide; After regulating the size of hatching compartment spaces, to hatching in cell the Incubating Solution added containing special first antibody, be sure oing to be pasted onto the histotomy of hatching bottom cell and can be immersed in the Incubating Solution containing special first antibody; Carry out hatching of histotomy on request; After first antibody has been hatched, sucking-off is containing the Incubating Solution of special first antibody, take out the camera incubata Space adjustment block inserting and hatch in frame in rectangular wall dovetail groove, unload the resilient clamp of hatching structure four limit that frame and microslide are stacked up and down, unload from microslide and hatch frame, routinely carrying out washing treatment is carried out to the histotomy be pasted onto on microslide; Need to carry out second antibody when hatching, repeat aforesaid operations, again formed camera incubata space size suitable do not leak hatch cell, add the Incubating Solution containing special second antibody, histotomy hatched; After having hatched, remove containing special second antibody Incubating Solution; Hatch frame under repeating aforesaid operations dismounting, the histotomy be pasted onto on microslide is washed, dewaters, transparent, mounting aftertreatment; After all process complete, examine under a microscope the immunohistochemical staining result of histotomy.
2. the using method of the detachable also microslide couveuse of volume adjustable according to claim 1, is characterized in that: the Space adjustment part of camera incubata Space adjustment block: put into and hatch cell, regulates the size in camera incubata space.
3. the using method of the detachable also microslide couveuse of volume adjustable according to claim 1, it is characterized in that: the swallow-tail form convex body of camera incubata Space adjustment block: insert in the dovetail groove of hatching on frame inwall, prevent camera incubata Space adjustment block from hatching little indoor migration.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410012882.3A CN103743899B (en) | 2014-01-13 | 2014-01-13 | Detachable and volume adjustable glass slide incubator |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410012882.3A Division CN103743899B (en) | 2014-01-13 | 2014-01-13 | Detachable and volume adjustable glass slide incubator |
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| Publication Number | Publication Date |
|---|---|
| CN104698163A CN104698163A (en) | 2015-06-10 |
| CN104698163B true CN104698163B (en) | 2016-04-27 |
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| CN201510052601.1A Expired - Fee Related CN104698163B (en) | 2014-01-13 | 2014-01-13 | The using method of the detachable also microslide couveuse of volume adjustable |
| CN201410012882.3A Expired - Fee Related CN103743899B (en) | 2014-01-13 | 2014-01-13 | Detachable and volume adjustable glass slide incubator |
| CN201510052659.6A Expired - Fee Related CN104678094B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency |
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| CN201410012882.3A Expired - Fee Related CN103743899B (en) | 2014-01-13 | 2014-01-13 | Detachable and volume adjustable glass slide incubator |
| CN201510052659.6A Expired - Fee Related CN104678094B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of the detachable also volume adjustable of experimental procedure convenience and high-efficiency |
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| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
| DE102017213923A1 (en) * | 2017-08-10 | 2019-02-14 | Robert Bosch Gesellschaft Für Medizinische Forschung Mbh | Device for cultivating tissue sections |
| DE102023201202A1 (en) * | 2023-02-14 | 2024-08-14 | Robert Bosch Gesellschaft Für Medizinische Forschung Mbh | Device for culturing a tissue section |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5364790A (en) * | 1993-02-16 | 1994-11-15 | The Perkin-Elmer Corporation | In situ PCR amplification system |
| WO1999058954A1 (en) * | 1998-05-13 | 1999-11-18 | Bayer Corporation | Optical spectroscopy sample cell |
| JP2002517725A (en) * | 1998-06-02 | 2002-06-18 | ヴェンタナ メディカル システムズ インコーポレイテッド | Method for staining biological specimens by combining unstable reagents on microscope slides |
| CN1138145C (en) * | 2001-04-27 | 2004-02-11 | 上海晶泰生物技术有限公司 | Multiple-sample microarray biochip |
| US7238521B2 (en) * | 2003-11-24 | 2007-07-03 | Biocept, Inc. | Microarray hybridization device having bubble-fracturing elements |
| EP2043785A2 (en) * | 2006-06-29 | 2009-04-08 | GE Healthcare Bio-Sciences AB | Chamber apparatus |
| CN202433235U (en) * | 2012-01-30 | 2012-09-12 | 上海海洋大学 | Clamp for fixing glass slides |
| CN203350255U (en) * | 2013-07-02 | 2013-12-18 | 武汉三鹰生物技术有限公司 | Adjustable horizontal incubation box |
| CN103344760B (en) * | 2013-07-23 | 2015-08-26 | 成都安铂奥金生物科技有限公司 | A kind of high flux method for immunohistochemical detection and Multi-example SABC check-out console |
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2014
- 2014-01-13 CN CN201510052601.1A patent/CN104698163B/en not_active Expired - Fee Related
- 2014-01-13 CN CN201410012882.3A patent/CN103743899B/en not_active Expired - Fee Related
- 2014-01-13 CN CN201510052659.6A patent/CN104678094B/en not_active Expired - Fee Related
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|---|---|
| CN104698163A (en) | 2015-06-10 |
| CN103743899B (en) | 2015-04-15 |
| CN104678094B (en) | 2016-04-27 |
| CN104678094A (en) | 2015-06-03 |
| CN103743899A (en) | 2014-04-23 |
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