CN211235190U

CN211235190U - Cell slide tissue staining operation vessel

Info

Publication number: CN211235190U
Application number: CN201921780994.8U
Authority: CN
Inventors: 梁玺; 何建明
Original assignee: Hebei Provincial Hospital Of Traditional Chinese Medicine
Current assignee: Hebei Provincial Hospital Of Traditional Chinese Medicine
Priority date: 2019-10-23
Filing date: 2019-10-23
Publication date: 2020-08-11
Anticipated expiration: 2029-10-23

Abstract

The utility model provides a cell slide tissue staining operation household utensils, include: the electrostatic film coating device comprises a bottom plate and a side wall plate surrounding the edge of the bottom plate, wherein an electrostatic film layer is arranged on the upper surface of the bottom plate. The bottom plate of the cell slide tissue staining operation vessel provided by the utility model is provided with the static film layer, so that the sealing film can be tiled, the problem that liquid is easy to overflow is effectively solved, meanwhile, the antibody liquid and the like are saved, and the cost is reduced; and simultaneously, the effective staining of cells around the slide is ensured. The inner wall is low, and transparent and colorless materials are used, so that the observation at any time in the operation process is facilitated.

Description

Cell slide tissue staining operation vessel

Technical Field

The utility model relates to a cell slide tissue staining technical field especially relates to a cell slide tissue staining operation household utensils.

Background

Cell slide tissue staining (immunohistochemical staining/immunofluorescence staining/HE staining, etc.) is an important, commonly used method for biomedical research. The current method is as follows:

1) the slides were placed in petri dishes or wells of petri plates and stained. The disadvantages are as follows: the creeper is difficult to take out due to smooth bottom and overhigh wall height, and a certain part is broken when the creeper is taken out; when the antibody liquid is added, the antibody liquid easily flows out of the slide, so that dyeing failure or waste of a large amount of precious antibodies is caused; due to the fact that the wall height of the culture dish or the culture plate is too high, observation is difficult to achieve under a microscope in the dyeing process, and observation and control cannot be achieved in the operation process; due to the fact that the wall height of the culture dish or the culture plate is too high, the front and the back of the slide cannot be clearly seen, dyeing failure is caused, or a large amount of precious antibodies are wasted; to stain cells around the slide, an increased amount of antibody is necessary, which results in waste and high cost.

2) The slide is placed on a common platform (e.g., a slide) and stained. The disadvantages are as follows: A. the cell slide is made of thin glass, the glass slide is glass or the like, and in the dyeing process, water solution needs to be added continuously, so that water is remained between the cell slide and the glass slide (or the like). The cell is difficult to climb, and cells on the cell are broken, dropped on the ground and damaged; and when the antibody solution is used, the liquid is easy to flow out of the center, so that dyeing failure, reagent waste and the like are caused. B. The glass slide is thin and brittle, is not easy to move and is easy to break in the clamping process. C. When the antibody liquid is added, more antibody liquid is added, and the antibody liquid is easy to flow out, so that dyeing failure is caused or a large amount of precious antibodies are wasted; less, drying up leads to staining failure, or surrounding cells fail to stain, leading to loss of some important data, and even leading to some unrealistic information. D. Cells around the slide are difficult to stain, and some important information cannot be obtained.

3) The slide is placed on a common operating platform (such as a glass slide), and an immunohistochemical wax pen is used for enclosing a part of the area for staining. The disadvantages are as follows: A. the cell slide is made of thin glass, the glass slide is glass or the like, and in the dyeing process, water solution needs to be added continuously, so that water is remained between the cell slide and the glass slide (or the like). The cell is difficult to climb, and cells on the cell are broken, dropped on the ground and damaged; and when the antibody solution is used, the liquid is easy to flow out of the center, so that dyeing failure, reagent waste and the like are caused. B. The glass slide is thin and brittle, is not easy to move and is easy to break in the clamping process. C. Cells around the slide cannot be stained, and some important information is lost.

During the dyeing process, the climbing piece needs to be clamped for many times. Therefore, whether the slide can be easily clamped is an important factor influencing the tissue staining success rate and the operation time. In addition, since the price of a staining reagent, particularly an antibody, is high, the use of an antibody solution or the like is reduced, and the cost of the experiment is directly determined. Particularly, the cell staining around the slide is carried out by placing the slide in a culture dish or a culture plate hole and using a large amount of antibody liquid, so that the cost is high due to the experiment.

The periphery of cells around the climbing sheet is not contacted with cells, the cells are different from the cells at the periphery of the cells in the climbing sheet, and meanwhile, the cells at the periphery of the climbing sheet are positioned at the boundary of a carrier, and the stress and the like are different from the periphery of the cells in the climbing sheet. Therefore, comparing the difference between the cells around the slide and the cells in the slide is of great significance in the study of the function and mechanism of cell-cell adhesion and contact, and the study of specific purposes. Therefore, the method has certain significance and practical value for efficiently, cheaply and stably staining cells around the slide.

At present, no satisfactory practical and effective auxiliary tool for immunohistochemistry/immunofluorescence staining of the cell slide exists, and the main problems of antibody saving and better staining of cells around the slide are solved.

SUMMERY OF THE UTILITY MODEL

The features and advantages of the present invention are set forth in part in the description which follows, or may be obvious from the description, or may be learned by practice of the invention.

In order to overcome the problems of the prior art, the utility model provides a cell slide tissue staining operation household utensils, include: the bottom plate and set up the end to end's on the bottom plate lateral wall board, the upper surface of bottom plate is equipped with the static rete.

Optionally, a groove is formed on the bottom plate.

Optionally, the number of the grooves is one, and the grooves are located in the middle of the bottom plate.

Optionally, the groove has a depth of 0.5-2 mm and a width of 1-4 mm.

Optionally, the electrostatic film layer is integrally formed with the base plate.

Optionally, the electrostatic film layer and the bottom plate are both made of the material of the electrostatic film layer.

Optionally, the cell-slide tissue staining operating vessel comprises a sealing film, and the sealing film is laid on the electrostatic film layer.

Optionally, the size of the sealing film is larger than the size of the bottom plate.

Optionally, the base plate is square.

Optionally, the cell slide tissue staining operating vessel comprises a large bottom plate, and the large bottom plate is composed of a plurality of bottom plates and a gap plate located between two adjacent bottom plates.

Optionally, the height of the side wall plate is 1-3 mm.

The utility model provides a cell slide tissue staining operation vessel, a bottom plate is provided with a static film layer, which can lead a sealing film (parafilm) to be spread, effectively solve the problem that liquid is easy to overflow, save antibody liquid and the like and reduce the cost; and simultaneously, the effective staining of cells around the slide is ensured. The inner wall is low, and transparent and colorless materials are used, so that the observation at any time in the operation process is facilitated.

The features and content of these solutions will be better understood by those skilled in the art from reading the present description.

Drawings

The advantages and mode of realisation of the invention will become more apparent hereinafter by describing in detail the invention with reference to the attached drawings, wherein the content shown in the drawings is only for explaining the invention, without constituting any limitation to the meaning of the invention, in which:

fig. 1 is a schematic structural diagram of a cell slide tissue staining operating vessel according to an embodiment of the present invention.

Fig. 2A is a cross-sectional view of the cell-slide tissue staining manipulation vessel shown in fig. 1 along a-a according to an embodiment of the present invention.

Fig. 2B is a cross-sectional view of the cell-slide tissue staining manipulation vessel shown in fig. 1 along a-a according to another embodiment of the present invention.

Fig. 3A is a cross-sectional view along B-B of the cell-slide tissue staining manipulation vessel shown in fig. 1 according to the embodiment of the present invention.

Fig. 3B is a cross-sectional view of the cell-slide tissue staining manipulation vessel shown in fig. 1 along B-B according to another embodiment of the present invention.

Fig. 4 is a schematic structural diagram of another cell-slide tissue staining operating vessel according to an embodiment of the present invention.

Detailed Description

As shown in fig. 1, fig. 2A and fig. 3A, the utility model provides a cell slide tissue staining operation vessel, which comprises a bottom plate 10 and side wall plates 20 arranged end to end on the bottom plate, wherein the upper surface of the bottom plate 10 is provided with an electrostatic film layer 11. In order to facilitate observation at any time in the dyeing process, the cell slide tissue dyeing operation vessel is made of transparent and colorless materials such as glass, plastic and the like.

In the embodiment, the side wall plate 20 is arranged around the edge of the bottom plate 10, the bottom plate 10 and the side wall plate 20 can be integrally formed, and in order to facilitate clamping of a slide, the cell-climbing tissue staining operating vessel has a low inner diameter and a depth of 1-3 mm; that is, the height of the side wall plate 20 is 1 to 3 mm. The arrangement of the side wall plate 20 provides conditions for placing a slide and ensuring that antibody liquid or the like does not overflow.

The base plate 10 is square. Because cell slide is carried out with 35mm culture dish, 6 ~ 48 orifice plates more, in order to adapt to corresponding slide, there are a plurality of specifications of cell slide tissue staining operation household utensils internal diameter side length, respectively 38 ~ 42mm (be applicable to 6 orifice plates or 35mm culture dish), 24 ~ 28mm (be applicable to 12 orifice plates), 12 ~ 16mm (be applicable to 48 orifice plates). It can be seen that the square vessel can be adapted to slides of various specifications.

In order to ensure that the antibody liquid and the like do not overflow out of the glass slide, cover the whole glass slide (including the periphery), and save the antibody liquid and the like, in the embodiment, the cell slide tissue staining operating vessel further comprises a sealing film with strong hydrophobicity, and the sealing film is flatly laid on the electrostatic film layer 11; the arrangement of the electrostatic film layer 11 can make the sealing film flatly and conveniently laid on the bottom. Each time the experiment is completed, a new sealing film can be replaced. The size of the sealing film is larger than that of the bottom plate, and preferably, the length of the sealing film is larger than the sum of 2 times of the height of the side wall plate and the side length of the bottom plate.

Preferably, the upper surface of the bottom plate 10 is provided with a groove 13, and the depth of the groove 13 is 0.5-2 mm, and the width of the groove 13 is 1-4 mm. The groove does not need to be too wide and too deep, and the bent forceps can be used conveniently to take out the slide.

In an embodiment of the present invention, the electrostatic film 11 can be integrally formed with the bottom plate 10, and in specific implementation, the whole bottom plate can be made of the material of the electrostatic film, as shown in fig. 2B and 3B. The material of the electrostatic film layer is, for example, PE or PVC, but the invention is not limited thereto. Since the groove 13 and the bottom plate are integrated, if the bottom plate is made of an electrostatic film material, the groove 13 is also provided with an electrostatic film layer, which does not affect the tiling of the sealing film, of course, if the bottom plate is not made of an electrostatic film material, the electrostatic film layer does not need to be arranged in the groove 13, and certainly, the arrangement of the electrostatic film layer in the groove 13 is also feasible.

On the basis of any of the above embodiments, in order to facilitate the simultaneous staining of a plurality of cell-climbing sheets, as shown in fig. 4, the cell-climbing sheet tissue staining operating vessel includes a large bottom plate, and the large bottom plate is composed of a plurality of bottom plates 10 and a gap plate 30 located between two adjacent bottom plates 10.

When the cell slide tissue staining operation vessel provided by the invention is used for carrying out experiments, the following steps can be adopted:

1) and culturing the cell slide.

2) Preparing a cell slide tissue staining operating vessel: a piece of sealing film (parafilm) slightly larger than the vessel is cut (the diameter of the sealing film is slightly larger than the length of the inner diameter of the cell slide tissue staining operating vessel plus 2 multiplied by the depth of the inner diameter), and the sealing film is flatly paved at the bottom of the cell slide tissue staining operating vessel.

3) The cell slide is placed in a cell slide tissue staining operating vessel (on a sealing film), and the side with the cultured cells faces upwards.

4) Staining was performed according to the procedure for staining-related staining.

5) After dyeing is finished, the sealing film is pressed along the groove by using the elbow forceps, so that a gap is formed between the sealing film and the glass slide; and then the bent tweezers are used for clamping the glass slide, and the glass slide is taken out and sealed.

Wherein, the step 4) takes immunofluorescence staining as an example, and comprises the following steps:

a) before placing the cell slide in a cell slide tissue staining operating vessel, the slide is clamped by an elbow forceps and is rinsed gently in 3 cylinders filled with PBS or other cleaning solutions. Or adding PBS or other cleaning liquid on the creeping sheet, waiting for 3-5 minutes, and sucking away the cleaning liquid; repeating the above steps for 2-3 times.

b) Adding a fixing solution (such as 4% paraformaldehyde) and fixing for 5-10 minutes or a proper time. The fixative solution is discarded.

c) Washing for 2-3 times (same as step a). ).

d) If necessary (e.g., staining of proteins in the cell membrane), membranes were broken (here, cell membrane) (0.1% Triton. TM. X-100/PBS). And (5) clamping the slide by using an elbow forceps, and drying by spinning.

e) And cleaning for 2-3 times.

f) Blocking with blocking solution (e.g., 5% BSA/PBS). And (5) clamping the slide by using an elbow forceps, and drying by spinning.

g) Adding enough primary antibody working solution and combining. (because the sealing film has very hydrophobic property, when water or aqueous solution is added, the liquid is completely on the glass slide and can not flow out of the glass slide to the sealing film, thus ensuring that cells around the glass slide are stained and ensuring that the effect can be achieved by a small amount of anti-working solution to play a role in saving.)

h) The relevant staining was performed according to the nature of the primary antibody or the instructions of a commercial tissue staining kit. This step is essentially the same as other tissue staining. (if the primary antibody itself is fluorescent, it is washed directly; if the primary antibody is not fluorescent, it is washed, and it is bound to the secondary antibody.)

When the cell slide immunohistochemistry/immunofluorescence staining method is used for cell slide immunohistochemistry, the method has the following advantages:

first, save antibody liquid, etc. There is no need to increase the amount of antibody fluid to the petri dish or well of the plate and submerge the entire coverslip. Compared with the method of putting the slide into a culture dish or a culture plate hole, the method saves more than three thirds of antibody liquid and the like.

And secondly, the clamping of the slide is simple and easy. Is obviously easier and more convenient than any method.

And thirdly, dyeing can be carried out under a microscope while observing. In particular in immunofluorescence staining, DAPI staining phase; or in the stage of immunohistochemical staining, developing or staining with eosin. The most satisfactory effect can be achieved by dyeing while observing.

The utility model provides a cell slide tissue staining operation household utensils, the inner wall is low, and has the recess bottom, conveniently presss from both sides and gets the slide. The inner surface of the container is provided with the electrostatic film layer combined with the sealing film (parafilm), so that the problem that liquid is easy to overflow is effectively solved, meanwhile, the antibody liquid and the like are saved, and the cost is reduced; and simultaneously, the effective staining of cells around the slide is ensured. The inner wall is low, and transparent and colorless materials are used, so that the observation at any time in the operation process is facilitated.

The preferred embodiments of the present invention have been described with reference to the accompanying drawings, and those skilled in the art can implement the present invention in various modifications without departing from the scope and spirit of the present invention. For instance, features illustrated or described as part of one embodiment, can be used with another embodiment to yield a still further embodiment. The above description is only a preferred and practical embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent changes made by using the contents of the specification and the drawings of the present invention are included in the scope of the present invention.

Claims

1. A cell slide tissue staining operating vessel, comprising: the bottom plate and set up the end to end's on the bottom plate lateral wall board, the upper surface of bottom plate is equipped with the static rete.

2. The vessel for cell-slide tissue staining operation according to claim 1, wherein the bottom plate is provided with a groove.

3. The vessel for cell-slide tissue staining operation according to claim 2, wherein the recess is one in the middle of the bottom plate.

4. The vessel for cell-slide tissue staining operation according to claim 2, wherein the groove has a depth of 0.5 to 2mm and a width of 1 to 4 mm.

5. The vessel for cell-slide tissue staining operation according to claim 1, wherein the electrostatic film layer is integrally formed with the bottom plate.

6. The vessel for cell-slide tissue staining operation according to claim 1, wherein the electrostatic film and the bottom plate are made of the material of the electrostatic film.

7. The vessel for cell-slide tissue staining operation according to claim 1, wherein the vessel comprises a sealing film laid on the electrostatic film layer.

8. The vessel for cell-slide tissue staining operation according to claim 7, wherein the sealing film has a size larger than that of the bottom plate.

9. The vessel for cell-slide tissue-staining operation according to claim 1, wherein the vessel comprises a large bottom plate, and the large bottom plate comprises a plurality of bottom plates and a gap plate between two adjacent bottom plates.

10. The vessel for cell-slide tissue staining manipulation according to claim 1, wherein the height of the sidewall plate is 1 to 3 mm.