CN2564585Y

CN2564585Y - Biochip hybridizing device

Info

Publication number: CN2564585Y
Application number: CN 02261951
Authority: CN
Inventors: 陈超; 王恒; 李莉; 赵洁
Original assignee: Shaanxi Lifegen Co Ltd
Current assignee: Shaanxi Lifegen Co Ltd
Priority date: 2002-07-15
Filing date: 2002-07-15
Publication date: 2003-08-06
Anticipated expiration: 2012-07-15

Abstract

Disclosed is a hybridization device of biological chips. The device is characterized in that a pedestal takes the hollow-framework structure, the size in the framework matches a glass slide, a liquid inlet hole is arranged on a upper cover, a matching closure plug is arranged on the liquid inlet hole, a sealing cog is positioned on the inner side of the upper cover, thus causing that a cultivation space is formed between the upper cover and the glass slide, and a sealing fastener fixes the upper cover and the pedestal after the upper cover is fastened on the pedestal. The device has the advantages that 1. the device takes an assembly structure, thus contributing to easy washing and repeated application; 2. the applied hybridization liquid can be less than 200ml during each hybridization process, thereby resulting in small hybridization liquid consumption and good sealing for ensuring safety of biological samples and reliable hybridization result; 3. due to simple operation and few processes, the hybridization result is not affected by artificial factors; and 4. the examination of the hybridization result can be completed through a general scanner of gene chips. The utility model can not only be applied in hybridization of gene chips, but also be adaptive to hybridization of various biological chips, such as albumin chips and organ chips.

Description

The biochip hybridization device

Technical field

The utility model belongs to the texture improvement of biochip hybridization device, is specifically related to biological experiment culture apparatus technical field.

Background technology

Along with the fast development of biotechnology and related discipline, Application of Biochips more and more widely.Can be in fields such as medicine, health, agricultural, environmental protection, the administration of justice as important detection means.Application of Biochips is that the biological specimen of itself and mark is hybridized, by the quantity of target molecule in the strong and weak judgement sample that detects hybridization signal.Though the hybridization principle of biochip is definite, the hybrid device that is used for biochip at present has various, yet the constructional feature of hybrid device has determined the operating method and the effect thereof of biochip hybridization.Existing biochip hybridization mode has following three kinds.

1, the coverslip method (http://cmgm.stanford.edu/pbrown/protocols/index.htm1) of the P.Brown of Stanford University laboratory announcement: hybridization solution is dropped on the cover glass, then cover glass is being fixed with on the slide glass of bioprobe, to seal with seal gum around the cover glass, hybridization is cultivated then.The advantage of this method is the consumption seldom (about 10 microlitres) of hybridization solution, shortcoming is that the cover glass that will hybridization solution be arranged is fixed on the slide glass probe location exactly, this operating process is very high to experimenter's requirement, so it is bigger that results of hybridization is influenced by human factor, and the requirement of seal operation is also very strict.

2, hybridizing box method (detecting the novel method of DNA chip false positive, false negative hybridization signal, number of patent application CN99104668.4): the DNA chip is positioned in the hybridizing box, adds and contain the hybridization solution of target gene to be measured in box.This method is easy to grasp, but hybridization solution submergence DNA chip (about 2 milliliters) fully, is very big waste for the hybridization solution of costliness.

3, hybridizing box and biochip packing box one (three-dimensional gene chip and manufacture method thereof, number of patent application CN99122375.6): this method can reduce to the hybridization solution consumption 200 microlitres, but the integral box device that it adopts can only be disposable use, the cost and the complexity of chip manufacturing have been increased, and when detecting, need tear box open and take out chip or use special chip scanner.

Summary of the invention

The purpose of this utility model is exactly the hybrid device of a kind of biochip of design, to overcome existing defective in the prior art.

The composition of the biochip hybridization device that the utility model is designed has: base, loam cake, ring close shape sealing fin, sealing plug, sealing fastener; Described base is the frame shape of hollow, and support bar is arranged in the frame, and the upper edge size is mated with the slide glass of carrying biochip in the frame, slide glass can be inserted in the frame; Loam cake size and base coupling is shaped on penetrating inlet opening at loam cake, and has the sealing plug with the inlet opening size match inlet opening to be stopped up from the loam cake outside; Be provided with ring in the inboard of loam cake and close shape sealing fin, make 1-4 rectangle grid by biochip zoning on the slide glass, this fin is after loam cake is placed on base, should make the airtight culture space of formation between loam cake inboard and the slide glass, this space can be an independently culture space of the 1-4 that separated by the sealing fin; After being placed on base, loam cake have sealing fastener that the two is fixed.Characteristics of the present utility model are that also described inlet opening has two at least in described each culture space, like this one of them as inlet opening another during as venting hole, can make feed liquor more smooth; In addition, if described feed liquor hole wall is shaped on from top to bottom step-like, then can avoid the feed liquor pin directly to enter the probe molecule that culture space scratches slide surface.

The utility model also has following constructional feature, can be provided with cover plate in the plane in described loam cake inboard, can be directly bonding or glued joint and the loam cake inboard by elastic strip, cover plate is provided with the corresponding inlet opening in inlet opening position with loam cake, this corresponding can be the different of quantity and size; At cover plate ring is set on the face of slide glass and closes shape sealing fin.The base of the designed hybrid device of the utility model and loam cake can be the branch body structures, also can be the conjoined structures of one-sided connection.

By said structure as can be seen, the using method of the biochip hybridization device that the utility model is designed is, can be not to be with slide glass as the hybrid device of commodity, and be the sterile packed through sterilization.Wherein on the packaging with on the capping mark or the diagram of culture space quantity and size should be arranged.Under the ultra-clean condition, open slide glass that loam cake will have biochip and insert in the frame of base (being shaped on facing up of chip), build loam cake, will cover with base with sealing fastener and closely fix, can be standby.The quantity and the size of wherein making the biochip zoning on slide glass should be complementary with the quantity and the size of sealing fin formation culture space, and the position is also corresponding.During use, under the ultra-clean condition, the sealing plug of hybrid device is pulled up, the hybridization solution for preparing is injected culture space by the feed liquor pin by inlet opening, again sealing plug is stopped up; Can hybridize cultivation; After finishing cultivation, hybrid device can be opened,, slide glass can be taken out and detect with finger top slide glass in place, space, base bottom.And this device can use after cleaning repeatedly.

The utility model is compared with the conventional device structure, has the following advantages: device 1. of the present utility model is a packaging assembly, is easy to clean, can use repeatedly; 2. in each crossover process, the hybridization solution of use can be less than 200 microlitres, and the hybridization solution consumption is little, and its good airproof performance can guarantee the safety of biological sample to make results of hybridization more reliable; 3. use simple to operate and program is few, results of hybridization is not influenced by human factor; 4. the detection of its results of hybridization can adopt general gene chip scanning instrument (as Genepix4000.Affymetrix428 etc.) to finish; 5. the utility model can not only be used for the hybridization of gene chip, also is applicable to the hybridization of multiple biochips such as protein chip, organization chip.

Description of drawings

Accompanying drawing 1 is the designed biochip hybridization device of the utility model (a kind of split structure example) structural representation, and accompanying drawing 2 is Fig. 1 structure assembling back outward appearance (internal organs is looked) synoptic diagram, accompanying drawing 3 be among Fig. 2 A-A to sectional view; Accompanying drawing 4 is the designed biochip hybridization device of the utility model (a kind of conjoined structure example) structural representation, and accompanying drawing 5 is Fig. 4 structure assembling back outward appearance (internal organs is looked) synoptic diagram, and accompanying drawing 6 is the frontview of Fig. 5, accompanying drawing 7 be among Fig. 5 B-B to sectional view.1 is slide glass among the figure, and 2 is inlet opening (or venting hole), and 3 for ring closes shape sealing fin, and 4 is base, and 5 is loam cake, and 6 is sealing plug, and 7 is sealing fastener, and 8 is cover plate, and 9 is elastic adhesive tape.

Embodiment

Describe embodiment of the present utility model in detail below in conjunction with accompanying drawing.

The designed biochip hybridization device of the utility model by loam cake, base, sealing plug, ring close shape sealing fin, these basic accessories of sealing fastener constitute, used slide glass can use as commodity purchasing separately after making biochip; Wherein said base and loam cake can be selected wooden or metal or plastics are that material is made, and also can be that the combination collocation of these materials uses; It is that material is made that sealing plug and sealing fin can adopt plastics, rubber, resin; And sealing fastener can adopt screw rod folder formula structure or screw threads locking structure or buckle structure or pin buckle structure etc.

Referring to accompanying

drawing

1,2,3, it is a kind of example that designed hybrid device divides body structure, and loam cake and base are separate, and base is the frame shape structure of hollow, (seeing lower part frame of broken lines among Fig. 1), and loam cake can be buckled in the frame of base.The step-like (see figure 3) that the inlet opening of loam cake is big up and small down can the secondary punching be finished also available mould once shaped; Can certainly adopt two layers of cover plate bonding finishing of punching respectively.The inboard set ring of loam cake closes shape sealing fin and is 4 grid-like (see figure 1)s of rectangle, and it can adopt, and elastic adhesive tape is bonding to form, and also can be and same material (as the plastics) once shaped of loam cake (or wherein a part of).And after loam cake was placed on base, formed space was to rely on the sealing fin to support and the (see figure 3) of sealing between loam cake inboard and the slide glass, and this space is exactly the enclosed space that hybridization is cultivated, and it highly can be generally 1mm in the 0.6-1.2mm scope.Fig. 1 and sealing fastener employed in figure 2 are screw rod folder formula structure; Certainly as can be seen from Figure 3, the device of this structure also can adopt the fastening piece of horizontal bolt rod structure.

Referring to accompanying

drawing

4,5,6,7, it is a kind of example of designed hybrid device conjoined structure, and loam cake is connected with bearing pin in a side with base, and base is the frame shape structure (seeing lower part frame of broken lines in the accompanying drawing 4) of hollow, and loam cake docks buckle closure with base.The inboard cover plate with the bonding horizontal of elastic adhesive tape of loam cake closes shape and seals fin (seeing Fig. 7 and 4) being wholely set ring towards slide glass one side and cover plate, and fin is the rectangle grid that 2 rings close among Fig. 4.Certainly after cover plate is buckled in slide surface, fin should have sealing function, is material so the making of cover plate can be adopted plastics or resin, for the enhanced leaktightness effect can be shaped on adhesive tape on the surface of fin; Fin itself should have certain elasticity, when elasticity is enough, then can adopt direct bonding way between cover plate and the loam cake, and need not increases buffering with elastic adhesive tape.As can be seen from Figure 7, the culture space between cover plate and the slide glass is 2.Inlet opening on the cover plate is corresponding with the inlet opening position of loam cake, but the inlet opening quantity of loam cake can more some more (see figure 4)s, can be beneficial to exhaust like this, and make hybridization enter requirement condition quickly; And the straight-through inlet opening of loam cake and cover plate then must have sealing plug to stop up inlet opening (seeing Fig. 5 and 7) to cover plate.As can be seen from Figure 6, the sealing fastener that this example adopted is a buckle structure, also can adopt other structures certainly.

In above-mentioned two kinds of specific exampless, many structures matched combined is again arranged, also can be used in the 1st example as covering plate structure, simple superstructure also can be used in the 2nd example, and disjunctor or split structure formation also are used interchangeably or the like.So protection of the present utility model is not limited to the concrete structure of above-mentioned two kinds of examples.

Claims

1, a kind of biochip hybridization device, employing has base, loam cake, ring to close shape sealing fin, sealing plug, sealing fastener, it is characterized in that, described base is the frame shape of hollow, support bar is arranged in the frame, the upper edge size is mated with the slide glass of carrying biochip in the frame, slide glass can be inserted in the frame; Loam cake size and base coupling is shaped on penetrating inlet opening at loam cake, and has the sealing plug with the inlet opening size match inlet opening to be stopped up from the loam cake outside; Be provided with ring in the inboard of loam cake and close shape sealing fin, make 1-4 rectangle grid by the zoning of biochip on the slide glass, this fin should make to constitute 1-4 airtight culture space between loam cake inboard and the slide glass after loam cake is placed on base; After being placed on base, loam cake have sealing fastener that the two is fixed.

2, device according to claim 1 is characterized in that, described inlet opening has two at least in each culture space.

3, device according to claim 2 is characterized in that, the height of described culture space is 0.6-1.2mm.

4, device according to claim 3 is characterized in that, the hole wall of described inlet opening has basipetal step.

According to the described arbitrary device of claim 1-4, it is characterized in that 5, described base and loam cake can be the branch body structures, or the conjoined structure of one-sided connection.

6, device according to claim 5, it just is that also the inboard of described loam cake is provided with cover plate, makes loam cake is two-layer structure; Cover plate and ring close shape sealing fin and are structure as a whole, and the inlet opening on the cover plate is corresponding with loam cake, and the inlet opening that loam cake and cover plate lead directly to has sealing plug to stop up inlet opening to cover plate.

7, device according to claim 6 is characterized in that, is provided with elastic adhesive tape between loam cake and cover plate.