CN102816685A - Biochip incubation reactor - Google Patents
Biochip incubation reactor Download PDFInfo
- Publication number
- CN102816685A CN102816685A CN2012103098801A CN201210309880A CN102816685A CN 102816685 A CN102816685 A CN 102816685A CN 2012103098801 A CN2012103098801 A CN 2012103098801A CN 201210309880 A CN201210309880 A CN 201210309880A CN 102816685 A CN102816685 A CN 102816685A
- Authority
- CN
- China
- Prior art keywords
- reaction
- incubation
- biochip
- reaction chamber
- boss
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
Abstract
The invention discloses a biochip incubation reactor, which includes a reaction chamber, and a reaction column installed to match the reaction chamber. The inner side of the reaction chamber top is provided with a boss, the inner side of which is provided with a sealing layer. The sealing layer, the boss and the reaction chamber are integrally molded, and the sealing layer has a hollow structure used as an incubation reaction chamber. A gap for installation of a biochip is disposed between the sealing layer and the upper surface of the reaction column. The reaction chamber top is equipped with a liquid injection hole for filling of an incubation reaction liquid into the incubation reaction chamber and a vent hole for discharging air in the incubation reaction chamber. The liquid injection hole and the vent hole both pass through the boss and are communicated with the incubation reaction chamber. The biochip incubation reactor disclosed in the invention has the advantages of reasonable structure and convenient assembling. When assembling is completed, the incubation reaction liquid can be injected timely according to the need. With the characteristics of simple and convenient operation, the biochip incubation reactor can meet the requirements of high-intensity oscillating incubation.
Description
Technical field
The invention belongs to biochip reaction device technical field, be specifically related to a kind of biochip incubation reaction device.
Background technology
Biochip (biochip) technology is the analytical technology of a Highgrade integration growing up phase early 1990s, can be divided into two big types of micro-array chip and micro-fluid chips according to its constructional feature.Wherein micro-array chip is the chip that is made up of the biomaterial microarray; Comprise DNA chip, protein chip, cell chip and organization chip etc.; Its ultimate principle all is that probe molecule is attached to certain surface of solid phase carriers; Then with solution in sample molecule carry out affine association reaction, strong and weak through the signal that detects after enzyme, resorcinolphthalein or ri amplify, and then the quantity of target molecule in the judgement sample.Biochip is integrated probe solid phase original position synthetic technology, photolithography technology, macromolecule synthesising technology, accurate control technique and laser scanning confocal microscopy, make synthetic, fixing highdensity ten hundreds of probe molecule and to hybridization signal carry out in real time, sensitivity, check and analysis accurately become practical.Biochip technology in the molecular biology research field, clinical medicine check field, field of biological pharmacy and environmental medicine field demonstrated great vitality.
Incubation step is one of important step in the biochip operation.In this process, the Nernst layer that free targeted molecular or identification molecule pass liquid-solid interface in the liquid phase is discerned each other with the probe molecule of solid surface and is combined, and the speed of reaction of this process and efficient receive solute and extends influence.For at utmost improving reaction efficiency and detection sensitivity, the routine process of hatching is to open wide or half opening wide under the system in biochemical incubator hold over night or auxiliary shaking table concussion several hours.This process length consuming time can not adapt to the demand of clinical detection; Hatch for a long time and possibly cause sample distortion, inactivation; Non-closed system can cause solution evaporation, produces edge effect influence experiment accuracy.Above variety of problems all produces very big influence to repeatability, the safety of experiment.Existing correlative study shows, be limited by liquid-solid barrier limit, even reaching leaving standstill of a couple of days hatches, its reaction efficiency still mixes not as short period of time HS hatches.
The biological incubation reaction device of prior art adopts earlier at the good biological chip of incubation reaction device internal fixing mostly; And then adding biochip incubation reaction liquid; Last in the mode that installs biochip incubation reaction device, but the biochip incubation reaction device fixed biochip less stable of this structure, and solvent is prone to loss; Operating process is more loaded down with trivial details, thus the effect that influence is hatched.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of rational in infrastructure, biochip incubation reaction device easy to assembly to above-mentioned deficiency of the prior art.After accomplishing, this reactor drum assembling can in time inject incubation reaction liquid as required, and simple to operate, convenient, can satisfy the requirement that high-intensity oscillation is hatched.When this reactor drum uses; Can directly incubation reaction liquid be injected the incubation reaction chamber through the liquid filling hole that the reaction cavity top is provided with; The liquid feeding mode is simple and convenient; Ventilating pit through the reaction cavity top is provided with is discharged the air in the incubation reaction chamber, makes fully to contact between incubation reaction liquid and the biochip, has avoided the influence of bubble to incubation reaction.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of biochip incubation reaction device, it is characterized in that, comprise reaction cavity and with the reaction column of the supporting installation of reaction cavity; Said reaction cavity inside top is provided with boss; Said boss inboard is provided with sealing ply; One-body molded and the sealing ply of said sealing ply, boss and reaction cavity has the hollow structure as the incubation reaction chamber; Be provided with the gap that is used to install biochip between the upper surface of said sealing ply and reaction column; Said reaction cavity top is provided with the liquid filling hole and the ventilating pit that is used to discharge incubation reaction chamber air that is used for to incubation reaction chamber filling incubation reaction liquid, and said liquid filling hole and ventilating pit all pass boss and be connected with the incubation reaction chamber.
Above-mentioned a kind of biochip incubation reaction device, said reaction column biside plate all closely contact with reaction cavity is inboard.
Above-mentioned a kind of biochip incubation reaction device is provided with raised ribs on the said reaction column biside plate, and reaction cavity each side has two grooves that match with raised ribs, and reaction column is installed in the reaction cavity through the raised ribs that snaps in the said groove.
Above-mentioned a kind of biochip incubation reaction device, the upper surface of said reaction column and the gap between the sealing ply are 0.2mm～10mm.
Above-mentioned a kind of biochip incubation reaction device, the thickness of said boss is 0.1mm～50mm.
Above-mentioned a kind of biochip incubation reaction device, the thickness of said sealing ply is 0.2mm～10mm.
Above-mentioned a kind of biochip incubation reaction device, the vertical section of said reaction cavity and reaction column is the U type.
Above-mentioned a kind of biochip incubation reaction device, said reaction column biside plate is positioned at the reaction cavity external portion and is corrugated.
The present invention compared with prior art has the following advantages:
1, biochip incubation reaction device of the present invention is rational in infrastructure, easy to assembly, and assembling can in time be injected incubation reaction liquid after accomplishing as required, and is simple to operate, convenient, can satisfy the requirement that high-intensity oscillation is hatched.
2, biochip incubation reaction device of the present invention in use can be through changing reaction cavity; Reach the purpose of effectively avoiding cross reaction; Practice thrift the reaction times, and reaction cavity is disposable use, can avoids the background interference that causes by the protein non-specific adsorption on the reaction cavity sidewall.
When 3, biochip incubation reaction device of the present invention uses; Can directly incubation reaction liquid be injected the incubation reaction chamber through the liquid filling hole that the reaction cavity top is provided with; The liquid feeding mode is simple and convenient; Ventilating pit through the reaction cavity top is provided with is discharged the air in the incubation reaction chamber, makes fully to contact between incubation reaction liquid and the biochip, has avoided the influence of bubble to incubation reaction.
Below in conjunction with accompanying drawing and embodiment, technical scheme of the present invention is done further detailed description.
Description of drawings
Fig. 1 is the perspective view of biochip incubation reaction device of the present invention.
Fig. 2 is the front view of biochip incubation reaction device of the present invention.
Fig. 3 is the vertical view of Fig. 2.
Fig. 4 is the A-A sectional view of Fig. 3.
Fig. 5 is the user mode synoptic diagram of biochip incubation reaction device of the present invention.
Description of reference numerals:
1-reaction cavity; 2-reaction column; 3-boss;
4-sealing ply; 5-incubation reaction chamber; 6-liquid filling hole;
7-ventilating pit; 8-raised ribs; 9-biochip.
Embodiment
Like Fig. 1, Fig. 2, Fig. 3 and biochip incubation reaction device shown in Figure 4, comprise reaction cavity 1 and with the reaction column 2 of reaction cavity 1 supporting installation; Said reaction cavity 1 inside top is provided with boss 3; Said boss 3 inboards are provided with sealing ply 4 (sealing ply can be annulus, Fang Huan, prismatic ring or trilateral ring); One-body molded and the sealing ply 4 of said sealing ply 4, boss 3 and reaction cavity 1 has the hollow structure as incubation reaction chamber 5; Be provided with the gap that is used to install biochip between the upper surface of said sealing ply 4 and reaction column 2; Said reaction cavity 1 top is provided with the liquid filling hole 6 and the ventilating pit 7 that is used to discharge incubation reaction chamber 5 air that is used for to incubation reaction chamber 5 filling incubation reaction liquid, and said liquid filling hole 6 all passes boss 3 with ventilating pit 7 and is connected with incubation reaction chamber 5; Said reaction column 2 biside plates all closely contact with reaction cavity 1 inboard; Be provided with raised ribs 8 on said reaction column 2 biside plates, reaction cavity 1 each side has two grooves that match with raised ribs 8, and reaction column 2 is installed in the reaction cavity 1 through the raised ribs 8 that snaps in the said groove; Gap between the upper surface of said reaction column 2 and the sealing ply 4 is 0.2mm～10mm, and said gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; The thickness of said boss 3 is 0.1mm～50mm, and said thickness is preferably 0.1mm, 5.0mm, 10.0mm, 15.0mm, 20.0mm, 25.0mm, 30.0mm, 35.0mm, 40.0mm, 45.0mm, 50mm; The thickness of said sealing ply 4 is 0.2mm～10mm, and said thickness is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; The vertical section of said reaction cavity 1 and reaction column 2 is the U type; Said reaction column 2 biside plates are positioned at reaction cavity 1 external portion and are corrugated.
As shown in Figure 5; The use of biochip incubation reaction device of the present invention is: the upper surface at reaction column 2 is pasted biochip 9; The reaction column that is pasted with biochip 92 is installed in the reaction cavity 1, and the upper surface of reaction column 2 is with being pressed to sealing ply 4 bottoms around the biochip 9; Under the condition that ventilating pit 7 opens wide, in incubation reaction chamber 5, inject incubation reaction liquid through liquid filling hole 6 then, the ventilating pit 7 that passes through of incubation reaction chamber 5 air is discharged; With liquid filling hole 6 and ventilating pit 7 sealings (adopt sealing paste stickup sealing or adopt the sealing plug sealing), place biochemical incubator or high-speed oscillator to carry out biochip mounted biochip incubation reaction device and hatch at last.
The above; It only is preferred embodiment of the present invention; Be not that the present invention is done any restriction, every according to inventing technical spirit to any simple modification, change and equivalent structure variation that above embodiment did, all still belong in the protection domain of technical scheme of the present invention.
Claims (8)
1. a biochip incubation reaction device is characterized in that, comprise reaction cavity (1) and with the reaction column (2) of the supporting installation of reaction cavity (1); Said reaction cavity (1) inside top is provided with boss (3); Said boss (3) inboard is provided with sealing ply (4); One-body molded and the sealing ply (4) of said sealing ply (4), boss (3) and reaction cavity (1) has the hollow structure as incubation reaction chamber (5); Be provided with the gap that is used to install biochip between the upper surface of said sealing ply (4) and reaction column (2); Said reaction cavity (1) top is provided with and is used for to the liquid filling hole (6) of incubation reaction chamber (5) filling incubation reaction liquid and is used to discharge the ventilating pit (7) of incubation reaction chamber (5) air, and said liquid filling hole (6) and ventilating pit (7) all pass boss (3) and be connected with incubation reaction chamber (5).
2. a kind of biochip incubation reaction device according to claim 1 is characterized in that, said reaction column (2) biside plate all closely contacts with reaction cavity (1) inboard.
3. a kind of biochip incubation reaction device according to claim 1; It is characterized in that; Be provided with raised ribs (8) on said reaction column (2) biside plate; Reaction cavity (1) each side has two grooves that match with raised ribs (8), and reaction column (2) is installed in the reaction cavity (1) through the raised ribs (8) that snaps in the said groove.
4. a kind of biochip incubation reaction device according to claim 1 is characterized in that the gap between the upper surface of said reaction column (2) and the sealing ply (4) is 0.2mm～10mm.
5. a kind of biochip incubation reaction device according to claim 1 is characterized in that the thickness of said boss (3) is 0.1mm～50mm.
6. a kind of biochip incubation reaction device according to claim 1 is characterized in that the thickness of said sealing ply (4) is 0.2mm～10mm.
7. a kind of biochip incubation reaction device according to claim 1 is characterized in that the vertical section of said reaction cavity (1) and reaction column (2) is the U type.
8. a kind of biochip incubation reaction device according to claim 1 is characterized in that, said reaction column (2) biside plate is positioned at reaction cavity (1) external portion and is corrugated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103098801A CN102816685A (en) | 2012-08-28 | 2012-08-28 | Biochip incubation reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103098801A CN102816685A (en) | 2012-08-28 | 2012-08-28 | Biochip incubation reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102816685A true CN102816685A (en) | 2012-12-12 |
Family
ID=47301139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103098801A Pending CN102816685A (en) | 2012-08-28 | 2012-08-28 | Biochip incubation reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102816685A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| CN1472339A (en) * | 2002-08-02 | 2004-02-04 | � 赵 | High-flux cell biological chip testing technology and reagent case |
| CN102226147A (en) * | 2011-05-30 | 2011-10-26 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN102435653A (en) * | 2011-09-05 | 2012-05-02 | 湖南大学 | Field effect transistor-based antibiotic medicine screening device and antibiotic medicine screening method |
| CN202766517U (en) * | 2012-08-28 | 2013-03-06 | 苏州偲聚生物材料有限公司 | Biological chip incubation reactor |
-
2012
- 2012-08-28 CN CN2012103098801A patent/CN102816685A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| CN1472339A (en) * | 2002-08-02 | 2004-02-04 | � 赵 | High-flux cell biological chip testing technology and reagent case |
| CN102226147A (en) * | 2011-05-30 | 2011-10-26 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
| CN102435653A (en) * | 2011-09-05 | 2012-05-02 | 湖南大学 | Field effect transistor-based antibiotic medicine screening device and antibiotic medicine screening method |
| CN202766517U (en) * | 2012-08-28 | 2013-03-06 | 苏州偲聚生物材料有限公司 | Biological chip incubation reactor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3985872B2 (en) | container | |
| CN101437616B (en) | Device and method for chemical, biochemical, biological and physical analysis, reaction, assay and more | |
| CN101006344B (en) | Automat system for handling microfluidic devices | |
| US9709562B2 (en) | Lateral flow assay devices for use in clinical diagnostic apparatus and configuration of clinical diagnostic apparatus for same | |
| EP2598245B1 (en) | Multi-well plate | |
| CN1974015A (en) | Containers and methods for the automated handling of a liquid | |
| CA2560450C (en) | Cuvette holder, cuvette array and analyzer comprising such components | |
| US20080160602A1 (en) | Microcolumn-based, high-throughput microfluidic device | |
| JP2009542222A (en) | Chamber device | |
| CN103191792B (en) | Microfluidic chip for microspheric multi-element biological detection | |
| CN1535182A (en) | Reagent delivery system | |
| EP3636738A1 (en) | Cartridge for nucleic acid detection | |
| JP2007068413A (en) | Microreactor for genetic testing | |
| JP2019501364A (en) | Microfluidic valve and chip or system with microfluidic valve | |
| CN101398437A (en) | Liquid transfering device, liquid transfering tube used in the device and series containers | |
| EP3377893A1 (en) | Method for measurement of live-cell parameters followed by measurement of gene and protein expression | |
| CN202766517U (en) | Biological chip incubation reactor | |
| CN102816685A (en) | Biochip incubation reactor | |
| CN202054829U (en) | Incubation reactor for biological chips | |
| KR100724316B1 (en) | Microfluidic Chip and Use Thereof | |
| CN102226147B (en) | Biochip incubation reactor | |
| CN211697839U (en) | Porous vapour-pressure type precision application of sample box | |
| Laurell et al. | The quest for high-speed and low-volume bioanalysis | |
| US20020187079A1 (en) | Robotic reservoir without liquid hangup | |
| CN101027545A (en) | Container |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121212 |