CN102226147B - Biochip incubation reactor - Google Patents
Biochip incubation reactor Download PDFInfo
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- CN102226147B CN102226147B CN 201110142518 CN201110142518A CN102226147B CN 102226147 B CN102226147 B CN 102226147B CN 201110142518 CN201110142518 CN 201110142518 CN 201110142518 A CN201110142518 A CN 201110142518A CN 102226147 B CN102226147 B CN 102226147B
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Abstract
The invention discloses a biochip incubation reactor, comprising a reaction chamber, an upper cover plate and a lower cover plate that are symmetrically arranged in the reaction chamber. An inner wall of the reaction chamber is equipped with a boss which is molded in one body with the reaction chamber and has a hollow structure to be used as an incubation reaction chamber. A gap used for installing a biochip is arranged between a lower surface of the upper cover plate and the boss; and a gap used for installing a biochip is also arranged between an upper surface of the lower cover plate and the boss. The biochip incubation reactor of the present invention has a simple and novel structure, and is convenient for operation. During the reaction, solution volatilization can be avoided, and high strength oscillation can be realized, so as to increase reaction efficiency, reduce incubation time, and enhance experiment sensitivity, singularity and reappearance. Both the upper cover plate and the lower cover plate of the reactor can be pasted with biochips, so that incubation of double biochips can be completed simultaneously to save samples at least by half compared with a traditional method.
Description
Technical field
The invention belongs to biochip reaction device technical field, be specifically related to a kind of biochip incubation reaction device.
Background technology
Biochip (biochip) technology is the analytical technology of a Highgrade integration growing up phase early 1990s, can be divided into micro-array chip and micro-fluid chip two large classes according to its constructional feature.Wherein micro-array chip is the chip that is made of the biomaterial microarray, comprise DNA chip, protein chip, cell chip and organization chip etc., its ultimate principle all is that probe molecule is attached to certain surface of solid phase carriers, then with solution in sample molecule carry out affine association reaction, strong and weak by the signal that detects after enzyme, fluorescein or radio isotope amplify, and then the quantity of target molecule in the judgement sample.Biochip is integrated probe solid phase original position synthetic technology, photolithography technology, macromolecule synthesising technology, accurate control technique and laser scanning confocal microscopy so that synthetic, fixing highdensity ten hundreds of probe molecule and to hybridization signal carry out in real time, sensitive, detect analysis accurately and become practical.Biochip technology in the molecular biology research field, clinical medicine check field, field of biological pharmacy and environmental medicine field demonstrated powerful vitality.
Incubation step is one of important step in the biochip operation.In this process, the targeted molecular that dissociates in the liquid phase or identification molecule pass the probe molecule of liquid-solid interface barrier and solid surface and mutually identify and combination, and the speed of reaction of this process and efficient are subject to solute and extend influence.For at utmost improving reaction efficiency and detection sensitivity, the routine process of hatching is to open wide or half opening wide under the system in biochemical cultivation case hold over night or auxiliary shaking table vibrates several hours.This process length consuming time can not adapt to the demand of clinical detection; Hatch for a long time and may cause sample sex change, inactivation; Non-closed system can cause solution evaporation, produces edge effect impact experiment accuracy.Above variety of problems all produces a very large impact repeatability, the reliability of experiment.Existing correlative study shows, be limited by liquid-solid barrier limit, even reaching leaving standstill of a couple of days hatches, its reaction efficiency still mixes not as the short period of time high strength hatches.Yet, realizing that it is to use automatically hybridization station that high strength is mixed the final method of hatching, the price at this hybridization station is generally 30,000～60, and 000 dollar, extremely expensive, be not suitable for promoting the use of.
Therefore, develop a kind of novel simple universal, fast and stable is adapted to biochip reaction device that high strength mixes not only can save time cost and Financial cost, and can improve sensitivity, stability and the circulation ratio of biochip.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of novelty simple in structure for above-mentioned deficiency of the prior art, easy to operate biochip incubation reaction device, and this reactor complete closed in the process of hatching can realize high-intensity oscillation.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of biochip incubation reaction device, it is characterized in that, and comprise that reaction cavity, symmetry are installed on upper cover plate and the lower cover in the reaction cavity; Be provided with boss on the described reaction cavity inwall, one-body molded and the boss of described boss and reaction cavity has the hollow structure as the incubation reaction chamber, be provided with between the lower capping of described upper cover plate and the boss for the gap that biochip is installed, also be provided with between the upper capping of described lower cover and the boss for the gap that biochip is installed.
Above-mentioned a kind of biochip incubation reaction device, described upper cover plate biside plate and lower cover biside plate all with the inboard close contact of reaction cavity.
Above-mentioned a kind of biochip incubation reaction device, lower capping and the gap between the boss of described upper cover plate are 0.2mm～10mm, upper capping and the gap between the boss of lower cover are 0.2mm～10mm.
Above-mentioned a kind of biochip incubation reaction device, the distance between described boss inwall and the reaction cavity inwall is 0.2mm～10mm.
Above-mentioned a kind of biochip incubation reaction device, the thickness of described boss is 0.2mm～10mm.
Above-mentioned a kind of biochip incubation reaction device, described upper cover plate biside plate and lower cover biside plate are provided with raised ribs, reaction cavity each side has two grooves that match with raised ribs, and upper cover plate and lower cover all are installed in the reaction cavity by the raised ribs that snaps in the described groove.
Above-mentioned a kind of biochip incubation reaction device, the cross section of described upper cover plate and lower cover is U-shaped.
Above-mentioned a kind of biochip incubation reaction device, the front side of described reaction cavity has the U-shaped groove of being convenient to add biochip incubation reaction liquid.
Above-mentioned a kind of biochip incubation reaction device, the both sides, front and back of described reaction cavity all have the U-shaped groove of being convenient to add biochip incubation reaction liquid.
Above-mentioned a kind of biochip incubation reaction device, the both sides, front and back of described reaction cavity all have laterally zygomorphic two U-shaped grooves of being convenient to add biochip incubation reaction liquid.
The present invention compared with prior art has the following advantages:
1, biochip incubation reaction device of the present invention novelty simple in structure is easy to operate.
2, biochip incubation reaction device of the present invention can be avoided the volatilization of solution in the reaction process, and can realize high-intensity oscillation (allowing the convertible high-intensity oscillation of 360 degree), thereby the raising reaction efficiency shortens incubation time, increases sensitivity, specificity and the circulation ratio of experiment.
3, biochip can both be pasted on the upper cover plate of biochip incubation reaction device of the present invention and the lower cover, therefore hatching of double biochip can be finished simultaneously, than traditional method saving sample over half at least.
4, biochip incubation reaction device of the present invention can Fast Installation and dismounting, and can utilize carriage to realize synchronous operation and the detection of many group reactions device, has saved labor service, has reduced energy consumption.
5, biochip incubation reaction device of the present invention in use can reach the purpose of effectively avoiding cross reaction by changing reaction cavity, saves the reaction times.And reaction cavity is disposable use, can avoid the background interference that is caused by the protein non-specific adsorption on the reaction cavity sidewall.
6, biochip incubation reaction device of the present invention is complete closed in the process of hatching, and has avoided the pollution of the nuisance in the reaction to the working space, has guaranteed staff's safety.
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Description of drawings
Fig. 1 is the perspective view of the first embodiment of the present invention.
Fig. 2 is the front view of the first embodiment of the present invention.
Fig. 3 is the vertical view of Fig. 2.
Fig. 4 is the A-A sectional view of Fig. 3.
Fig. 5 is the perspective view of the second embodiment of the present invention.
Fig. 6 is the front view of the second embodiment of the present invention.
Fig. 7 is the vertical view of Fig. 6.
Fig. 8 is the A-A sectional view of Fig. 7.
Fig. 9 is the perspective view of the third embodiment of the present invention.
Figure 10 is the front view of the third embodiment of the present invention.
Figure 11 is the vertical view of Figure 10.
Figure 12 is the A-A sectional view of Figure 11.
Figure 13 is that the first of the present invention is used view.
Figure 14 is that the second of the present invention uses view.
Figure 15 is the third use view of the present invention.
Description of reference numerals:
The 1-reaction cavity; The 2-upper cover plate; The 3-lower cover;
The 4-boss; The 5-raised ribs; 6-incubation reaction chamber;
The 7-biochip; The 8-seal washer.
Embodiment
Such as Fig. 1, Fig. 2, Fig. 3 and biochip incubation reaction device shown in Figure 4 comprise that reaction cavity 1, symmetry are installed on upper cover plate 2 and the lower cover 3 in the reaction cavity 1; Be provided with boss 4 on described reaction cavity 1 inwall, one-body molded and the boss 4 of described boss 4 and reaction cavity 1 has the hollow structure as incubation reaction chamber 6, be provided with between the lower capping of described upper cover plate 2 and the boss 4 for the gap that biochip is installed, also be provided with between the upper capping of described lower cover 3 and the boss 4 for the gap that biochip is installed; Described upper cover plate 2 biside plates and lower cover 3 biside plates all with reaction cavity 1 inboard close contact; Lower capping and the gap between the boss 4 of described upper cover plate 2 are 0.2mm～10mm, and described gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Upper capping and the gap between the boss 4 of lower cover 3 are 0.2mm～10mm, and described gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Distance between described boss 4 inwalls and reaction cavity 1 inwall is 0.2mm～10mm, and described distance is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; The thickness of described boss 4 is 0.2mm～10mm, and described thickness is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Described upper cover plate 2 biside plates and lower cover 3 biside plates are provided with raised ribs 5, reaction cavity 1 each side has two grooves that match with raised ribs 5, and upper cover plate 2 and lower cover 3 all are installed in the reaction cavity 1 by the raised ribs 5 that snaps in the described groove; The cross section of described upper cover plate 2 and lower cover 3 is U-shaped; The both sides, front and back of described reaction cavity 1 all have the U-shaped groove of being convenient to add biochip incubation reaction liquid.
Such as Fig. 5, Fig. 6, Fig. 7 and biochip incubation reaction device shown in Figure 8 comprise that reaction cavity 1, symmetry are installed on upper cover plate 2 and the lower cover 3 in the reaction cavity 1; Be provided with boss 4 on described reaction cavity 1 inwall, one-body molded and the boss 4 of described boss 4 and reaction cavity 1 has the hollow structure as incubation reaction chamber 6, be provided with between the lower capping of described upper cover plate 2 and the boss 4 for the gap that biochip is installed, also be provided with between the upper capping of described lower cover 3 and the boss 4 for the gap that biochip is installed; Described upper cover plate 2 biside plates and lower cover 3 biside plates all with reaction cavity 1 inboard close contact; Lower capping and the gap between the boss 4 of described upper cover plate 2 are 0.2mm～10mm, and described gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Upper capping and the gap between the boss 4 of lower cover 3 are 0.2mm～10mm, and described gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Distance between described boss 4 inwalls and reaction cavity 1 inwall is 0.2mm～10mm, and described distance is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; The thickness of described boss 4 is 0.2mm～10mm, and described thickness is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Described upper cover plate 2 biside plates and lower cover 3 biside plates are provided with raised ribs 5, reaction cavity 1 each side has two grooves that match with raised ribs 5, and upper cover plate 2 and lower cover 3 all are installed in the reaction cavity 1 by the raised ribs 5 that snaps in the described groove; The cross section of described upper cover plate 2 and lower cover 3 is U-shaped; The front side of described reaction cavity 1 has the U-shaped groove of being convenient to add biochip incubation reaction liquid.
Such as Fig. 9, Figure 10, Figure 11 and biochip incubation reaction device shown in Figure 12 comprise that reaction cavity 1, symmetry are installed on upper cover plate 2 and the lower cover 3 in the reaction cavity 1; Be provided with boss 4 on described reaction cavity 1 inwall, one-body molded and the boss 4 of described boss 4 and reaction cavity 1 has the hollow structure as incubation reaction chamber 6, be provided with between the lower capping of described upper cover plate 2 and the boss 4 for the gap that biochip is installed, also be provided with between the upper capping of described lower cover 3 and the boss 4 for the gap that biochip is installed; Described upper cover plate 2 biside plates and lower cover 3 biside plates all with reaction cavity 1 inboard close contact; Lower capping and the gap between the boss 4 of described upper cover plate 2 are 0.2mm～10mm, and described gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Upper capping and the gap between the boss 4 of lower cover 3 are 0.2mm～10mm, and described gap is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Distance between described boss 4 inwalls and reaction cavity 1 inwall is 0.2mm～10mm, and described distance is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; The thickness of described boss 4 is 0.2mm～10mm, and described thickness is preferably 0.2mm, 1.0mm, 2.0mm, 3.0mm, 4.0mm, 5.0mm, 6.0mm, 7.0mm, 8.0mm, 9.0mm, 10mm; Described upper cover plate 2 biside plates and lower cover 3 biside plates are provided with raised ribs 5, reaction cavity 1 each side has two grooves that match with raised ribs 5, and upper cover plate 2 and lower cover 3 all are installed in the reaction cavity 1 by the raised ribs 5 that snaps in the described groove; The cross section of described upper cover plate 2 and lower cover 3 is U-shaped; The both sides, front and back of described reaction cavity 1 all have laterally zygomorphic two U-shaped grooves of being convenient to add biochip incubation reaction liquid.
As shown in figure 13, the first using method of biochip incubation reaction device of the present invention is all to paste biochip on the upper capping of the lower capping of upper cover plate 2 and lower cover 3, the use procedure of the method is: the lower cover 3 that will be pasted with first biochip 7 is installed in the reaction cavity 1, lower cover 3 will be pressed to boss 4 bottoms (for the biochip of hard substrate around the biochip 7, can between biochip and boss, install the seal washer that matches with boss additional), the mounting means of lower cover 3 is: pinch lower cover 3 both sides lower cover 3 is sent in the reaction cavity 1, the raised ribs 5 of lower cover 3 both sides snaps in the groove of reaction cavity 1, and lower cover 3 firmly is installed in the reaction cavity 1; Then the hollow structure for as biochip incubation reaction chamber 6 to boss 4 adds biochip incubation reaction liquid; The upper cover plate 2 that will be pasted with again biochip 7 is installed in the reaction cavity 1 according to the mode with lower cover 3 symmetries, upper cover plate 2 will be pressed to boss 4 tops (for the biochip of hard substrate, can install the seal washer that matches with boss additional between biochip and boss) around the biochip 7; Placing biochemical cultivation case or high-speed oscillator to carry out biochip one or more mounted biochip incubation reaction devices at last hatches.
As shown in figure 14, the second using method of biochip incubation reaction device of the present invention is only to paste biochip at the lower capping of upper cover plate 2, the use procedure of the method is: install the seal washer 8 that matches with boss 4 additional at lower cover 3 first, lower cover 3 is installed in the reaction cavity 1; Then the hollow structure for as biochip incubation reaction chamber 6 to boss 4 adds biochip incubation reaction liquid; The upper cover plate 2 that will be pasted with again biochip 7 is installed in the reaction cavity 1 according to the mode with lower cover 3 symmetries, upper cover plate 2 will be pressed to boss 4 tops (for the biochip of hard substrate, can install the seal washer that matches with boss additional between biochip and boss) around the biochip 7; Placing biochemical cultivation case or high-speed oscillator to carry out biochip one or more mounted biochip incubation reaction devices at last hatches.
As shown in figure 15, the third using method of biochip incubation reaction device of the present invention is only to paste biochip at the upper capping of lower cover 3, the use procedure of the method is: the lower cover 3 that will be pasted with first biochip 7 is installed in the reaction cavity 1, lower cover 3 will be pressed to boss 4 bottoms (for the biochip of hard substrate, can install the seal washer that matches with boss additional between biochip and boss) around the biochip 7; Then the hollow structure for as biochip incubation reaction chamber 6 to boss 4 adds biochip incubation reaction liquid; Install the seal washer 8 that matches with it additional at boss 4 again, upper cover plate 2 is installed in the reaction cavity 1 according to the mode with lower cover 3 symmetries; Placing biochemical cultivation case or high-speed oscillator to carry out biochip one or more mounted biochip incubation reaction devices at last hatches.
Biochip incubation reaction device of the present invention can be avoided the volatilization of solution in the reaction process, and can realize high-intensity oscillation (allowing the convertible high-intensity oscillation of 360 degree), thereby the raising reaction efficiency shortens incubation time, increases sensitivity, specificity and the circulation ratio of experiment.Biochip can both be pasted on the upper cover plate of reactor and the lower cover, therefore hatching of double biochip can be finished simultaneously, than traditional method saving sample over half at least.Reactor in use can reach the purpose of effectively avoiding cross reaction by changing reaction cavity, saves the reaction times, and reaction cavity is disposable use, can avoid the background interference that is caused by the protein non-specific adsorption on the reaction cavity sidewall.
The above; it only is preferred embodiment of the present invention; be not that the present invention is done any restriction, every any simple modification, change and equivalent structure of above embodiment being done according to the invention technical spirit changes, and all still belongs in the protection domain of technical solution of the present invention.
Claims (8)
1. biochip incubation reaction device, it is characterized in that, comprise that reaction cavity (1), symmetry are installed on upper cover plate (2) and the lower cover (3) in the reaction cavity (1), described upper cover plate (2) biside plate and lower cover (3) biside plate all with the inboard close contact of reaction cavity (1); The front side of described reaction cavity (1) has the U-shaped groove of being convenient to add biochip incubation reaction liquid, be provided with boss (4) on described reaction cavity (1) inwall, one-body molded and the boss (4) of described boss (4) and reaction cavity (1) has the hollow structure as incubation reaction chamber (6), be provided with between the lower capping of described upper cover plate (2) and the boss (4) for the gap that biochip is installed, also be provided with between the upper capping of described lower cover (3) and the boss (4) for the gap that biochip is installed.
2. a kind of biochip incubation reaction device according to claim 1, it is characterized in that, gap between the lower capping of described upper cover plate (2) and the boss (4) is 0.2mm～10mm, and the gap between the upper capping of lower cover (3) and the boss (4) is 0.2mm～10mm.
3. a kind of biochip incubation reaction device according to claim 1 is characterized in that, the distance between described boss (4) inwall and reaction cavity (1) inwall is 0.2mm～10mm.
4. a kind of biochip incubation reaction device according to claim 1 is characterized in that the thickness of described boss (4) is 0.2mm～10mm.
5. a kind of biochip incubation reaction device according to claim 1, it is characterized in that, described upper cover plate (2) biside plate and lower cover (3) biside plate are provided with raised ribs (5), reaction cavity (1) each side has two grooves that match with raised ribs (5), and upper cover plate (2) and lower cover (3) all are installed in the reaction cavity (1) by the raised ribs (5) that snaps in the described groove.
6. a kind of biochip incubation reaction device according to claim 5 is characterized in that, the cross section of described upper cover plate (2) and lower cover (3) is U-shaped.
7. a kind of biochip incubation reaction device according to claim 1 is characterized in that the both sides, front and back of described reaction cavity (1) all have the U-shaped groove of being convenient to add biochip incubation reaction liquid.
8. a kind of biochip incubation reaction device according to claim 1 is characterized in that, the both sides, front and back of described reaction cavity (1) all have laterally zygomorphic two U-shaped grooves of being convenient to add biochip incubation reaction liquid.
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| CN 201110142518 CN102226147B (en) | 2011-05-30 | 2011-05-30 | Biochip incubation reactor |
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| CN 201110142518 CN102226147B (en) | 2011-05-30 | 2011-05-30 | Biochip incubation reactor |
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| CN102226147B true CN102226147B (en) | 2013-01-23 |
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| CN102816685A (en) * | 2012-08-28 | 2012-12-12 | 苏州偲聚生物材料有限公司 | Biochip incubation reactor |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448723A (en) * | 2002-03-29 | 2003-10-15 | 成都夸常科技有限公司 | Biological chip capable of being disassembled and assembled |
| CN1825121A (en) * | 2005-02-24 | 2006-08-30 | 穆海东 | Plastic chip |
| CN2863809Y (en) * | 2005-12-15 | 2007-01-31 | 华东理工大学 | Biological chip reactor |
| CN202054829U (en) * | 2011-05-30 | 2011-11-30 | 苏州偲聚生物材料有限公司 | Incubation reactor for biological chips |
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- 2011-05-30 CN CN 201110142518 patent/CN102226147B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448723A (en) * | 2002-03-29 | 2003-10-15 | 成都夸常科技有限公司 | Biological chip capable of being disassembled and assembled |
| CN1825121A (en) * | 2005-02-24 | 2006-08-30 | 穆海东 | Plastic chip |
| CN2863809Y (en) * | 2005-12-15 | 2007-01-31 | 华东理工大学 | Biological chip reactor |
| CN202054829U (en) * | 2011-05-30 | 2011-11-30 | 苏州偲聚生物材料有限公司 | Incubation reactor for biological chips |
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