CN2393885Y - Reusable closed bacteria culture - Google Patents
Reusable closed bacteria culture Download PDFInfo
- Publication number
- CN2393885Y CN2393885Y CN 99238174 CN99238174U CN2393885Y CN 2393885 Y CN2393885 Y CN 2393885Y CN 99238174 CN99238174 CN 99238174 CN 99238174 U CN99238174 U CN 99238174U CN 2393885 Y CN2393885 Y CN 2393885Y
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- CN
- China
- Prior art keywords
- loam cake
- repeatedly used
- utility
- ventilation
- model
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a reusable closed bacteria cultivation incubator; particularly an aseptic detection technique; the utility model provides an accurate, fast and aseptic test method which is especially suitable for medicine, biological products and medical appliances. The utility model is composed of a dismountable bacteria cultivation filter, a breeding pipe and a ventilated upper cover, wherein, the dismountable bacteria cultivation filter, the breeding pipe and the ventilated upper cover are orderly connected through screw threads from the bottom up and are respectively made of high temperature-proof and high pressure-proof materials. As the utility model is reusable, cost is lowered, and environmental pollution is prevented.
Description
The utility model relates to aseptic detection technique, and being particularly useful for a kind ofly provides accurately quick, antipollution aseptic means of testing for medicine, biological products and medical device.
Existing two kinds of filtrations and cultural method and device thereof.Traditional a kind of be earlier sample to be filtered by strainer, millipore filtration is divided into three parts again, cultivation in the container of a certain amount of substratum (pipe or bottle) is housed respectively, this method trivial operations, and easily cause the exogenous pollution of testing process.Another kind is a Chinese patent: colony culture device (ZL 95203829.3), disclosed technology is that the filtration of sample and culturing process are all carried out in a kind of closed colony culture device, this method operation is comparatively easy, and can fully avoid the exogenous pollution of testing process, but its primary structure shell is owing to can not be split, and with material be can not withstand high temperatures (120 ℃), the transparent plastics manufacturing of high pressure (0.1MPa), thereby can only disposablely use, this incubator cost is greatly improved, and cause comparatively serious environmental pollution.
The purpose of this utility model just is to overcome the shortcoming and defect that prior art exists, can split and provide a kind of, can tolerate the closed colony culture device of high temperature (120 ℃) high pressure (0.1MPa), it can pass through to change filter membrane and effective sterilizing, thereby it can be used repeatedly.
But the purpose of this utility model is achieved in that the transparent plastics of selecting withstand high temperatures (120 ℃), high pressure (0.1MPa) on material for use, and as polycarbonate (bisphenol A-type), and its consistency and elasticity meets the requirements, not cracky; Structurally be that closed incubator is split into three parts: bacteria gathering filter, culture tube, ventilation loam cake.Place millipore filtration and sealing-ring between bacteria gathering filter and the culture tube, be connected by screw thread; Place air filter membrane and sealing-ring between ventilation loam cake and the culture tube, be connected by screw thread.After one-time detection is finished, three parts can be split, washing reapposes millipore filtration or air filter membrane, be assembled into one after, can use repeatedly through effective sterilizing.
Describe in detail below in conjunction with drawings and Examples.
Fig. 1 is the utility model structural representation, 1-bacteria gathering filter wherein, 2-culture tube, 3-ventilation loam cake, 4-go out sample pipe, 5-seal cap, 6-porose backing plate, 7-millipore filtration; 8-volume indicatrix (100ml), 9-sample introduction pipe, 10-air filtration film, 11-ventpipe, 12-seal cap, 13-sample introduction conduit, 14-clips, 15-screw thread, 16-screw thread.
As shown in Figure 1, the utility model mainly is made up of bacteria gathering filter 1, culture tube 2 and ventilation loam cake 3, connects by screw thread 15,16 successively from top to bottom.
Bacteria gathering filter 1 is a round base, and bottom center has one outstanding to go out sample pipe 4, and tight with seal cap 5 lids, it places porose backing plate 6 above end.On porose backing plate 6, place millipore filtration 7.
Culture tube 2 is the big little taper shape of, and sample introduction pipe 9 is arranged at top, puts a short sample introduction conduit 13, and a clips 14 is arranged on it.The top of culture tube 2 also has the cylindrical ventilation loam cake pedestal that ventilating pit is arranged in the middle of, goes up with ventilation loam cake 3 to be connected.In addition, there is a ventpipe 11 in the central authorities of ventilation loam cake 3, and are tight with seal cap 12 lids.
By above explanation as can be known, the utility model is simple in structure, splits easy to assemblyly, can use repeatedly, not only reduces cost, and prevents the pollution of the environment.The utility model connects and can be used with peristaltic pump or negative pressure pump by suitable conduit, constitutes the full closed aseptic detection system.
Claims (4)
1, a kind of repeatedly used closed colony culture device is characterized in that it is made up of the bacteria gathering filter that can split (1), culture tube (2) and ventilation loam cake (3), from bottom to up successively by screw thread (15,16) connection; But all use the material manufacturing of withstand high temperatures (120 ℃) high pressure (0.1MPa).
2, by the described a kind of repeatedly used closed colony culture device of claim 1, it is characterized in that bacteria gathering filter (1) is a round base, bottom center has one outstanding to go out sample pipe (4), and is tight with seal cap (5) lid; It is placed with porose backing plate (6) above end, be placed with millipore filtration (7) on porose backing plate (6).
3, by the described a kind of repeatedly used sealing collection bacterium formula incubator of claim 1, it is characterized in that culture tube (2) for going up little taper shape once greatly, sample introduction pipe (9) is arranged at top, puts a short sample introduction conduit (13), also has a clips (14) on it; The top of culture tube (2) also has the cylindrical ventilation loam cake pedestal that ventilating pit is arranged in the middle of, goes up with ventilation loam cake (3) to be connected.
4, by the described a kind of repeatedly used closed colony culture device of claim 1, there is a ventpipe (11) in loam cake (3) central authorities that it is characterized in that ventilating, cover seal cap (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99238174 CN2393885Y (en) | 1999-07-27 | 1999-07-27 | Reusable closed bacteria culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99238174 CN2393885Y (en) | 1999-07-27 | 1999-07-27 | Reusable closed bacteria culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2393885Y true CN2393885Y (en) | 2000-08-30 |
Family
ID=34025668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99238174 Expired - Fee Related CN2393885Y (en) | 1999-07-27 | 1999-07-27 | Reusable closed bacteria culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2393885Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100341998C (en) * | 2003-07-31 | 2007-10-10 | 廉翰林 | One-pressing transshipping medium vessel |
| CN105300737A (en) * | 2015-05-08 | 2016-02-03 | 中国科学院沈阳应用生态研究所 | Long-term in-situ collection method and device for forest tree root exudates |
-
1999
- 1999-07-27 CN CN 99238174 patent/CN2393885Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100341998C (en) * | 2003-07-31 | 2007-10-10 | 廉翰林 | One-pressing transshipping medium vessel |
| CN105300737A (en) * | 2015-05-08 | 2016-02-03 | 中国科学院沈阳应用生态研究所 | Long-term in-situ collection method and device for forest tree root exudates |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |