CN105259240B - The quick spot sample device of PCR primer electrophoresis - Google Patents
The quick spot sample device of PCR primer electrophoresis Download PDFInfo
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- CN105259240B CN105259240B CN201510650641.6A CN201510650641A CN105259240B CN 105259240 B CN105259240 B CN 105259240B CN 201510650641 A CN201510650641 A CN 201510650641A CN 105259240 B CN105259240 B CN 105259240B
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Abstract
The present invention relates to a kind of quick spot sample device of PCR primer electrophoresis, do not absorb water including base and the sample-adding scraps of paper, multiple PCR pipe standing grooves are provided with base, it is flexibly connected on base and is provided with multiple sample-adding scraps of paper fix bars, do not absorb water to be loaded on the scraps of paper and be provided with multiple sample-adding grooves, the sample-adding scraps of paper that do not absorb water are jagged near one jiao of setting for being loaded scraps of paper fix bar connection end, the sample-adding scraps of paper that do not absorb water are pressed abd fixed on base by scraps of paper fix bar, the quick spot sample device of PCR primer electrophoresis also includes connecting rod, and multiple sample-adding scraps of paper fix bars are connected with connecting rod.The present invention can reduce point sample process, improve operating efficiency with 8 samples of time point.The present invention is provided with sample fixed component, efficiently solves the problem of sample is easily chaotic, prevents the situation that sample pollutes caused by sample mixes from occurring.In addition, the present invention can open and depress simultaneously multiple sample-adding scraps of paper fix bars, it is easy to use.
Description
Technical field
The present invention relates to a kind of quick spot sample device of PCR primer electrophoresis.
Background technology
PCR primer electrophoresis is that the gene for detecting the fast and low-costs such as whether PCR reactions succeed, PCR primer clip size expands
Increase the operation of point sample and the interpretation of speed influence electrophoresis efficiency and result before laboratory common technology, PCR primer electrophoresis.At present
PCR primer electrophoresis point sample is without fixing device, and operation differs between each laboratory, and main apparatus is single channel pipettor and liquid relief
Device suction nozzle, PE gloves or preservative film.The step of PCR primer electrophoresis, is as follows, and PE gloves or preservative film are layered on to smooth table first
On face, then with single channel pipettor electrophoresis by 1-5ml sample-loading buffers（loading buffer）Point is in PE gloves or fresh-keeping
On film, each one point of PCR primer takes 1-5ml PCR primers and PE hands followed in turn by single channel pipettor from each PCR pipe
The electrophoresis sample-loading buffer put on set or preservative film mixes the latter agarose or polyacrylamide gel for clicking and entering electrophoresis apparatus
In sample well.Existing PCR primer electrophoresis point sample efficiency is low, and when PCR primer quantity is big, above printing operation takes time and effort,
Used it is difficult to meet, the PE gloves or preservative film of existing point sample are not allowed to be fixed easily, and easily wave, therefore put superincumbent PCR productions
Thing is not only easily chaotic, and drops or wave if the PE gloves or preservative film of having put PCR primer hold, different PCR productions
Thing, which will mix, causes sample to pollute, and all that has been achieved is spoiled.
The content of the invention
The technical problem to be solved in the present invention is：A kind of multichannel point sample is provided, operating efficiency is improved, effectively prevents sample
Product, which mix, causes the quick spot sample device of PCR primer electrophoresis of sample confusion, solves the problem of above-mentioned prior art is present.
Solving the technical scheme of above-mentioned technical problem is：A kind of quick spot sample device of PCR primer electrophoresis, including base and not
It is provided with to be flexibly connected on multiple PCR pipe standing grooves, the base on the water suction sample-adding scraps of paper, the base and is provided with N number of sample-adding
Multiple sample-adding grooves are provided with scraps of paper fix bar, the sample-adding scraps of paper that do not absorb water, and the sample-adding scraps of paper that do not absorb water are near adding
One jiao of setting of sample scraps of paper fix bar connection end is jagged, and the sample-adding scraps of paper that do not absorb water are pressed abd fixed on base by scraps of paper fix bar
On, the value of the N is 3~20.
PCR pipe standing groove on described base is identical with the sample-adding number of recesses not absorbed water on the sample-adding scraps of paper, and PCR pipe is put
The quantity for putting groove and sample-adding groove is 8, and the separation on PCR pipe standing groove is identical with the separation for being loaded groove.
The quick spot sample device of PCR primer electrophoresis also includes connecting rod, and described N number of sample-adding scraps of paper fix bar is and connecting rod
Connection.
Due to using said structure, the invention has the advantages that：
1st, multiple PCR pipe standing grooves are provided with base of the invention, eight connecting legs can be placed, while the sample-adding paper that do not absorb water
Multiple sample-adding grooves of respective amount are set on piece, can be loaded simultaneously from eight connecting leg point samples to not absorbing water on the scraps of paper using the volley of rifle fire
Multiple sample-adding grooves in, i.e., can once reduce point sample process with 8 samples of time point, improve operating efficiency.
2nd, the present invention is provided with multiple sample-adding scraps of paper fix bars, and the sample-adding scraps of paper that multiple can not absorb water simultaneously are fixed on bottom
Point sample is carried out on seat, the sample-adding scraps of paper that do not absorb water are due to there is sample-adding scraps of paper fix bar to be fixed, after sample-adding or sample-adding process
In, sample is not easily shifted, efficiently solves the problem of sample is easily chaotic, prevents that sample is chaotic and dirty caused by sample mixes
The situation of dye occurs.
3rd, multiple sample-adding scraps of paper fix bars of the invention are connected on connecting rod simultaneously, are easy to open and depress multiple sample-adding paper
Piece fix bar, it is easy to use.
Below, the technical characteristic of the quick spot sample device of PCR primer electrophoresis of the present invention is made in conjunction with the accompanying drawings and embodiments into
The explanation of one step.
Brief description of the drawings
Fig. 1：The quick spot sample device structural representation of PCR primer electrophoresis of the present invention.
Fig. 2：The sample-adding scraps of paper structural representation that do not absorb water of the present invention.
Fig. 3：The understructure schematic diagram of the present invention.
Fig. 4：The base stereogram of the present invention.
Fig. 5：Fig. 1 A-A sectional views.
Fig. 6：Fig. 5 B portions enlarged drawing.
In figure：1- bases, 2- is not absorbed water the sample-adding scraps of paper, and 21- sample-adding grooves, 3-PCR pipe standing grooves, the 4- sample-adding scraps of paper are fixed
Bar, 5- connecting rods.
Embodiment
Embodiment 1：A kind of quick spot sample device of PCR primer electrophoresis, as shown in figs 1 to 6, including base 1 and do not absorb water plus
It is provided with multiple PCR pipe standing grooves 3, the base 1 to be flexibly connected on the sample scraps of paper 2, the base 1 and is provided with 8 sample-adding paper
Piece fix bar 4, that is, being loaded scraps of paper fix bar 4 can be lifted and depress, and multiple sample-addings are provided with the sample-adding scraps of paper 2 that do not absorb water
Groove 21, the sample-adding scraps of paper 2 that do not absorb water set jagged 22 near one jiao of sample-adding scraps of paper fix bar connection end, do not absorb water
The sample-adding scraps of paper 2 are pressed abd fixed on base 1 by scraps of paper fix bar 4.The breach 22 for being loaded the scraps of paper 2 that do not absorb water is consolidated with being loaded the scraps of paper
The connection end shape of fixed pole 4 is corresponding, and the sample-adding scraps of paper 2 that enable not absorb water more easily are pressed abd fixed on bottom by scraps of paper fix bar 4
On seat 1.
In the present embodiment, the PCR pipe standing groove 3 on described base is counted with the sample-adding groove 21 on the sample-adding scraps of paper that do not absorb water
Amount is identical, and the pitch of holes on PCR pipe standing groove 3 is identical with the pitch of holes for being loaded groove 21, PCR pipe standing groove and sample-adding groove
Quantity is 8, can place eight connecting legs.As one kind conversion, described PCR pipe standing groove and the quantity of sample-adding groove can also
Differ, PCR pipe standing groove can increase or reduce as needed with being loaded the quantity of groove.
In the present embodiment, the sample-adding scraps of paper 2 that do not absorb water are disposable.
In the present embodiment, the quick spot sample device of PCR primer electrophoresis also includes connecting rod 5, and described N number of sample-adding scraps of paper are consolidated
Fixed pole 4 is connected with connecting rod 5, is easy to while opening and depressing multiple sample-adding scraps of paper fix bars 4., can also as one kind conversion
Connecting rod 5 is not provided with, multiple sample-adding scraps of paper fix bars 4 is individually opened and is depressed.
As one kind conversion of the present embodiment, the quantity of sample-adding scraps of paper fix bar 4 can increase according to actual needs or
Reduce, generally 3~20.
Mechanism：Eight connecting legs are placed in PCR pipe standing groove 3 first, the sample-adding scraps of paper 2 that then will not absorb water are by paper
Piece fix bar 4 is pressed abd fixed on base 1, using the volley of rifle fire simultaneously from eight connecting leg point samples to do not absorb water sample-adding the scraps of paper on it is multiple plus
In sample groove, scraps of paper fix bar 4 can once be opened after terminating with 8 samples of time point, point sample, the excellent sample-adding that do not absorb water will be put
The scraps of paper 2 take out.
Claims (3)
1. a kind of quick spot sample device of PCR primer electrophoresis, it is characterised in that：Including base（1）Do not absorb water the sample-adding scraps of paper（2）,
The base（1）On be provided with multiple PCR pipe standing grooves（3）, the base（1）Upper flexible connection is provided with N number of sample-adding scraps of paper
Fix bar（4）, the sample-adding scraps of paper that do not absorb water（2）On be provided with multiple sample-adding grooves（21）, the sample-adding scraps of paper that do not absorb water（2）
One jiao of setting near sample-adding scraps of paper fix bar connection end is jagged（22）, do not absorb water the sample-adding scraps of paper（2）Fixed by the scraps of paper
Bar（4）It is pressed abd fixed on base（1）On, the value of the N is 3~20, PCR pipe standing groove（3）On separation and be loaded it is recessed
Groove（21）Separation it is identical.
2. the quick spot sample device of PCR primer electrophoresis according to claim 1, it is characterised in that：PCR on described base
Pipe standing groove（3）With the sample-adding groove not absorbed water on the sample-adding scraps of paper（21）Quantity is identical, the number of PCR pipe standing groove and sample-adding groove
Amount is 8.
3. the quick spot sample device of PCR primer electrophoresis according to claim 1 or 2, it is characterised in that：The PCR primer electrophoresis
Quick spot sample device also includes connecting rod（5）, described N number of sample-adding scraps of paper fix bar（4）And connecting rod（5）Connection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510650641.6A CN105259240B (en) | 2015-10-10 | 2015-10-10 | The quick spot sample device of PCR primer electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510650641.6A CN105259240B (en) | 2015-10-10 | 2015-10-10 | The quick spot sample device of PCR primer electrophoresis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105259240A CN105259240A (en) | 2016-01-20 |
| CN105259240B true CN105259240B (en) | 2017-09-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510650641.6A Active CN105259240B (en) | 2015-10-10 | 2015-10-10 | The quick spot sample device of PCR primer electrophoresis |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2258618Y (en) * | 1996-02-12 | 1997-07-30 | 中国人民解放军第四军医大学 | Sample trough for multi-function PCR gene amplification instrument |
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| CN201990685U (en) * | 2011-03-31 | 2011-09-28 | 杭州金源生物技术有限公司 | Novel compound PCR (polymerase chain reaction) plate |
| CN205301234U (en) * | 2015-10-10 | 2016-06-08 | 柳州市妇幼保健院 | Quick sample application device of PCR result electrophoresis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2394175B1 (en) * | 2009-02-09 | 2016-02-03 | caprotec bioanalytics GmbH | Devices, systems and methods for separating magnetic particles |
| WO2013086509A1 (en) * | 2011-12-08 | 2013-06-13 | Duke University | Flow chamber assembly and methods of using the same |
-
2015
- 2015-10-10 CN CN201510650641.6A patent/CN105259240B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2258618Y (en) * | 1996-02-12 | 1997-07-30 | 中国人民解放军第四军医大学 | Sample trough for multi-function PCR gene amplification instrument |
| CN2564585Y (en) * | 2002-07-15 | 2003-08-06 | 陕西西大北美基因股份有限公司 | Biochip hybridizing device |
| CN201990685U (en) * | 2011-03-31 | 2011-09-28 | 杭州金源生物技术有限公司 | Novel compound PCR (polymerase chain reaction) plate |
| CN205301234U (en) * | 2015-10-10 | 2016-06-08 | 柳州市妇幼保健院 | Quick sample application device of PCR result electrophoresis |
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| Publication number | Publication date |
|---|---|
| CN105259240A (en) | 2016-01-20 |
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