CN2258618Y - Sample trough for multi-function PCR gene amplification instrument - Google Patents
Sample trough for multi-function PCR gene amplification instrument Download PDFInfo
- Publication number
- CN2258618Y CN2258618Y CN 96235626 CN96235626U CN2258618Y CN 2258618 Y CN2258618 Y CN 2258618Y CN 96235626 CN96235626 CN 96235626 CN 96235626 U CN96235626 U CN 96235626U CN 2258618 Y CN2258618 Y CN 2258618Y
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- frid
- trough
- pcr
- cooling
- platform
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Abstract
The utility model belongs to a biological device which is a trough for a multifunctional PCR gene amplification instrument. The utility model is divided into a left section and a right section, wherein, a blind hole is arranged on a trough plate in the left section, and the blind hole is used for carrying out a test in a test tube PCR; besides, the test tube is arranged in the blind hole; a platform is arranged on a trough plate in the right section, and the platform is used for carrying out in situ PCR, in situ hybridization and immunohistochemical tests; besides, a glass slide is arranged on the platform. The heating section of each of the trough plates is an electrical heating element, and the electrical heating element is inserted into a main body of each of the trough plates; the cooling section of each of the trough plates is a water cooling trough positioned on the bottom surface of each of the trough plates. The utility model has the advantages of fast speed in raising and lowering temperature, preferable uniformity and wide range of application.
Description
The utility model belongs to the biology device, is a kind of sample cell of pcr gene amplification instrument.
Pcr gene amplification instrument is used for the PCR.Existing P CR gene-amplificative instrament only has simple function, promptly can only carry out test tube PCR.Their sample cell mainly is made up of frid, heating part and cooling segment, and wherein frid is provided with the blind hole of many sockets test tube.The sample cell of the disclosed pcr amplification instrument of Chinese patent CN2136435 adopts electrical heating and heat radiator cooling, and heater element is an electric hot plate between frid and heat radiator, so the cooling velocity of this sample cell is slow, and wayward.The sample cell of the disclosed pcr amplification instrument of Chinese patent CN2162466 is made thermal source with light source, carries out heating and cooling by high-power fan with high velocity air, and the existing problem of this sample cell is that structure is complicated, and heating and chilling temperature are wayward.In addition, as previously mentioned, these two kinds of sample cells can only be made test tube PCR, can't observe the DNA that increased or the relation between RNA and the eucaryotic cell structure pathology, promptly can not be original position PCR.Be that histotomy is placed in the aluminium lunch box when being original position PCR in the past, carry in the water-bath of three different temperatures with manual method, carry out gene magnification, obvious this method is not only time-consuming, and the accuracy of time and temperature be difficult to control, influenced the gene magnification effect greatly.
The purpose of this utility model provides a kind ofly can be test tube PCR, can do the sample cell of the multi-functional pcr gene amplification instrument of original position PCR, molecular hybridization in situ and immunohistochemical test again.
The solution that realizes the utility model purpose is: the sample cell of the multi-functional pcr gene amplification instrument that is provided comprises the heating part and the cooling segment of frid, frid.Improvements of the present utility model are that frid is divided into left and right two districts, and wherein frid left side district is provided with the blind hole that inserts test tube, and the right district of frid is provided with the platform of placing microslide; Said heating part is to insert in the frid body from the side and surperficial heating and the temperature-sensing element that scribbles heat-conducting silicone grease; Said cooling segment is a water-cooling groove of being close to the frid bottom surface.The left side district of above-mentioned frid makes test tube PCR usefulness, and the platform in right district is placed microslide, can carry out pcr gene amplification, i.e. original position PCR to histotomy.The right side district of frid also can do molecular hybridization in situ and immunohistochemical test in addition.
Compared with prior art, the utility model inserts the frid body with heating element, and adopts water cooling, so heating-cooling speed is very fast, the homogeneity of temperature is also better, and has shortened cycle period greatly, has improved amplification efficiency.In addition, this sample cell not only can be test tube PCR and original position PCR, also can do molecular hybridization in situ and immunohistochemical test, has increased the usable range of pcr gene amplification instrument.
Below in conjunction with accompanying drawing content of the present utility model is illustrated.
Fig. 1 is cross-sectional figure of the present utility model.
Fig. 2 is the vertical view of Fig. 1.
Fig. 3 is the cut-open view at Figure 1A-A place.
Fig. 4 is the distribution schematic diagram of Fig. 1 water-cooling groove.
As shown in Figure 1 and Figure 2, frid 1 is an aluminium, is made up of 2 He You districts, left side district, 3 two parts, and they are embedded in the groove of sample cell pedestal 4.Frid left side district 2 is provided with 96 tapered blind hole 5, is used to place test tube; The right district 3 of frid is provided with the platform 6 of placing microslide, and the both sides of platform 6 respectively are provided with tank 7, are used to keep the moistening of reaction chamber.
Frid adopts Electric heating, and as shown in Figure 3: the side of frid body is provided with many rounds 8, and heating 9 is placed in the hole 8, and resistance-type temperature-sensing element 10 also is placed in the hole 8.In frid left side district 2, heating 9 can improve the homogeneity of heating like this between adjacent two row's blind holes 5.Heating 9 adopts the surface to scribble the heating wire of heat-conducting silicone grease.Be provided with water-cooling groove 11,12 above the sample cell pedestal 4, form the cooling-water duct of sealing between they and the frid bottom surface, be provided with sealing gasket 13 (referring to Fig. 3) therebetween.In order to improve the homogeneity of cooling, water-cooling groove 11,12 is a serpentine channel, and it distributes as shown in Figure 4.
Numbering among Fig. 1: the water-cooling groove water inlet in 14-frid left side district, the water-cooling groove water inlet in the right district of 15-frid.
Claims (3)
1. the sample cell of a multi-functional pcr gene amplification instrument, the heating part and the cooling segment that comprise frid (1), frid, it is characterized in that frid (1) is divided into left and right two districts (2,3), wherein frid left side district (2) is provided with the blind hole (5) that inserts test tube, and the right district of frid (3) is provided with the platform (6) of placing microslide; Said heating part is to insert in the frid body from the side and surperficial heating (9) and the temperature-sensing element (10) that scribbles heat-conducting silicone grease; Said cooling segment is a water-cooling groove (11,12) of being close to the frid bottom surface.
2. sample cell according to claim 1 is characterized in that said water-cooling groove (11,12) is a serpentine channel.
3. sample cell according to claim 1 and 2 is characterized in that in frid left side district (2), and heating (9) is positioned between adjacent two row's blind holes (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96235626 CN2258618Y (en) | 1996-02-12 | 1996-02-12 | Sample trough for multi-function PCR gene amplification instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96235626 CN2258618Y (en) | 1996-02-12 | 1996-02-12 | Sample trough for multi-function PCR gene amplification instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2258618Y true CN2258618Y (en) | 1997-07-30 |
Family
ID=33911623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96235626 Expired - Fee Related CN2258618Y (en) | 1996-02-12 | 1996-02-12 | Sample trough for multi-function PCR gene amplification instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2258618Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363001B (en) * | 2008-08-22 | 2012-06-27 | 金银杏生物科技(北京)有限公司 | Sliding mode heat transfer media plate PCR instrument |
| CN105259240A (en) * | 2015-10-10 | 2016-01-20 | 柳州市妇幼保健院 | Rapid sample application device for PCR product electrophoresis |
| CN110257244A (en) * | 2019-04-23 | 2019-09-20 | 武汉观锐生物科技有限公司 | A kind of couveuse |
-
1996
- 1996-02-12 CN CN 96235626 patent/CN2258618Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101363001B (en) * | 2008-08-22 | 2012-06-27 | 金银杏生物科技(北京)有限公司 | Sliding mode heat transfer media plate PCR instrument |
| CN105259240A (en) * | 2015-10-10 | 2016-01-20 | 柳州市妇幼保健院 | Rapid sample application device for PCR product electrophoresis |
| CN105259240B (en) * | 2015-10-10 | 2017-09-22 | 柳州市妇幼保健院 | The quick spot sample device of PCR primer electrophoresis |
| CN110257244A (en) * | 2019-04-23 | 2019-09-20 | 武汉观锐生物科技有限公司 | A kind of couveuse |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |