CN212228963U - Refrigeration film-transferring groove - Google Patents
Refrigeration film-transferring groove Download PDFInfo
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- CN212228963U CN212228963U CN202020960525.0U CN202020960525U CN212228963U CN 212228963 U CN212228963 U CN 212228963U CN 202020960525 U CN202020960525 U CN 202020960525U CN 212228963 U CN212228963 U CN 212228963U
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Abstract
The application discloses refrigeration commentaries on classics membrane groove includes: the film rotating groove is arranged in the inner shell, and a closed interlayer is arranged between the inner shell and the outer shell; the top of the inner shell is higher than the top surface of the outer shell, and the top surface of the inner shell is open and extends out of the outer shell; the water outlet of the interlayer is communicated with the water inlet pipeline of the water tank; the water outlet of the water tank is communicated with the water inlet pipeline of the interlayer; the semiconductor refrigerator is arranged on the outer wall of the water tank; the refrigerating aluminum block of the semiconductor refrigerator extends into the water tank. Through set up the intermediate layer on changeing the membrane groove outer wall to let in the circulation comdenstion water in the intermediate layer, make the comdenstion water at the intermediate layer inner loop, do benefit to change the heat dissipation of membrane in-process.
Description
Technical Field
The application relates to a refrigeration membrane transferring groove, and belongs to the field of western blotting methods.
Background
Western-blot is an important technical means for biological protein detection, is also called immunoblot assay, is proposed in 1979 by the bioscience of michelia fraderi, switzerland, is a commonly used experimental method in molecular biology, biochemistry and immunogenetics, and is now widely applied to various aspects such as gene expression research on protein level, antibody activity detection and early disease diagnosis.
Membrane transfer is a key step that affects whether western blotting is successful or not. In the process of film transfer, after the power supply is switched on, the current is large, the heat is seriously generated, and the temperature of the film transfer liquid is increased by the generated heat. The temperature of the film transferring liquid is increased to influence the film transferring efficiency and the stability of experimental results.
The existing cooling means of laboratory personnel are as follows: through set up the ice box in changeing the membrane groove, place the trash ice outside changeing the membrane groove simultaneously, cool down to changeing the membrane groove.
But this operation is comparatively troublesome, can't realize simultaneously moreover to the synchronous cooling of the liquid in the tunica mucosa ventriculi, influences the cooling homogeneity to all produce certain influence to experimental efficiency and experimental result.
SUMMERY OF THE UTILITY MODEL
The application provides a refrigeration rotary film groove for solving the problems that the temperature of a rotary film groove in the prior art is difficult to accurately control and the temperature fluctuation influences the stability and accuracy of a western blotting test result.
The application provides a refrigeration commentaries on classics membrane groove includes: the film rotating groove is arranged in the inner shell, and a closed interlayer is arranged between the inner shell and the outer shell;
the top of the inner shell is higher than the top surface of the outer shell, and the top surface of the inner shell is open and extends out of the outer shell;
the water outlet of the interlayer is communicated with the water inlet pipeline of the water tank;
the water outlet of the water tank is communicated with the water inlet pipeline of the interlayer;
the semiconductor refrigerator is arranged on the outer wall of the water tank;
the refrigerating aluminum block of the semiconductor refrigerator extends into the water tank.
Preferably, the interlayer water outlet is arranged on the upper side wall of the shell;
the water inlet of the water tank is arranged on the side wall of the upper part of the water tank.
Preferably, the water tank comprises a first pipeline, wherein the first pipeline is arranged on the side wall of the shell, and the first pipeline is communicated with the water outlet of the interlayer and the water inlet of the water tank.
Preferably, a first pump is provided on the first conduit.
Preferably, the interlayer water inlet is arranged on the lower side wall of the shell;
the water outlet of the water tank is arranged on the side wall of the lower part of the water tank.
Preferably, the water inlet device comprises a second pipeline, the second pipeline is arranged on the side wall of the shell, and the first end of the second pipeline is communicated with the water inlet of the interlayer;
the second end of the second pipeline is communicated with the water outlet of the water tank.
Preferably, a second pump is provided on the second conduit.
Preferably, the method further comprises the following steps: and the opening of the inner shell is provided with a cover.
Preferably, the method comprises the following steps: the temperature sensor is arranged on the inner side wall of the inner shell and is opposite to the condensed water outlet on the outer shell;
the display is arranged on the outer wall of the shell and is electrically connected with the temperature sensor.
Preferably, a layer of thermal insulation material is disposed on the outer wall of the water tank.
The beneficial effects that this application can produce include:
1) the application provides a membrane groove is changeed in refrigeration through setting up the intermediate layer on changeing the membrane groove outer wall to let in the circulation comdenstion water in the intermediate layer, make the comdenstion water at the intermediate layer inner loop, do benefit to the heat that changes the membrane in-process and give off. And can continuously take away the heat produced in the film rotating groove, realize the uniform and continuous cooling of the inner space of the film rotating groove, and improve the cooling uniformity of the film rotating liquid, thereby improving the accuracy and stability of the experimental result.
2) The application provides a refrigeration commentaries on classics membrane groove adopts this commentaries on classics membrane groove to enable commentaries on classics membrane liquid and maintains and stabilize at lower temperature, can solve the wet a series of problems that changes the membrane in-process heat production and bring of western blotting experiment. The film transferring groove can be used for uniformly refrigerating film transferring liquid and improving the film transferring efficiency. The adoption of the film rotating groove does not need to prepare crushed ice, saves the time of scientific research workers, and simultaneously improves the working efficiency.
3) The application provides a membrane groove is changeed in refrigeration sets up the semiconductor cooler on the condensate water tank outer wall through with the intermediate layer pipeline intercommunication, can realize the comdenstion water cooling to the circulation return water tank high-efficiently, energy-conservingly.
Drawings
FIG. 1 is a schematic front view of a refrigeration film-transferring tank provided by the present application;
illustration of the drawings:
10. a housing; 11. an interlayer; 12. an inner shell; 13. a film transferring groove; 131. a temperature sensor; 201. a first pump; 202. a second pump; 21. a water tank; 22. a semiconductor refrigerator; 221. and (5) refrigerating the aluminum block.
Detailed Description
The present application will be described in detail with reference to examples, but the present application is not limited to these examples.
Referring to fig. 1, the present application provides a refrigeration wiped film tank, comprising: the device comprises an outer shell 10, an inner shell 12, a water tank 21 and a semiconductor refrigerator 22, wherein a film rotating groove 13 is arranged in the inner shell 12, and a closed interlayer 11 is arranged between the inner shell 12 and the outer shell 10. The top of the inner shell 12 is higher than the top of the outer shell 10, and the top of the inner shell 12 is open and extends out of the outer shell 10.
The outer shell 10 is sleeved outside the inner shell 12 and is connected with the outer wall of the inner shell 12 in a sealing manner. The membrane transferring device can be put in the membrane transferring groove 13 for transferring the membrane.
The water outlet of the interlayer 11 is communicated with the water inlet pipeline of the water tank 21; the water outlet of the water tank 21 is communicated with the water inlet pipeline of the interlayer 11; the water tank 21 stores therein condensed water, which circulates between the interlayer 11 and the water tank 21.
The semiconductor refrigerator 22 is arranged on the outer wall of the water tank 21; the refrigeration aluminum block 221 of the semiconductor refrigerator 22 extends into the water tank 21. The refrigeration aluminum block 221 is stretched into the water tank 21 to cool the condensed water in the water tank 21, so that the temperature of the water flowing out of the water tank 21 is lower, and the heat exchange efficiency of the film rotating groove 13 is improved.
The electrophoresis tank module of the device is an integrated interlayer 11 module, and heat generated by the working of the inner layer can be dissipated through cooling water circulating in the outer space, so that the membrane transferring liquid in the inner shell 12 maintains a stable lower temperature. The semiconductor refrigeration and water circulation power module of the device can provide cooling water for the electrophoresis tank module, and the water circulation speed of the semiconductor refrigeration and water circulation power module can be regulated and controlled.
Preferably, the water outlet of the interlayer 11 is arranged on the upper side wall of the shell 10; an inlet of the water tank 21 is provided on an upper side wall of the water tank 21. With this arrangement, the circulation path of the condensed water in the jacket 11 can be extended, and the cooling effect can be enhanced.
Preferably, a first pipeline is arranged on the side wall of the shell 10, and the first pipeline is communicated with the water outlet of the interlayer 11 and the water inlet of the water tank 21. The first end of the first pipeline is connected with the water outlet of the interlayer 11; the second end of the first pipe is connected to the inlet of the water tank 21. With this arrangement, the circulation path of the condensed water in the jacket 11 can be extended, and the cooling effect can be enhanced.
Preferably, the water inlet of the interlayer 11 is arranged on the lower side wall of the shell 10; the water outlet of the water tank 21 is provided on the lower side wall of the water tank 21. With this arrangement, the circulation path of the condensed water in the jacket 11 can be extended, and the cooling effect can be enhanced.
Preferably, a second pipeline is arranged on the side wall of the shell 10, and a first end of the second pipeline is communicated with a water inlet of the interlayer 11; the second end of the second pipeline is communicated with the water outlet of the water tank 21. With this arrangement, the circulation path of the condensed water in the jacket 11 can be extended, and the cooling effect can be enhanced.
Preferably, a first pump 201 is provided on the first pipeline. Can take the comdenstion water out from intermediate layer 11 through setting up first pump 201 to provide the required power of comdenstion water circulation, improve heat exchange efficiency, in time take away the heat that changes the membrane tank and produce.
Preferably, a second pump 202 is disposed on the second conduit. Can go into the intermediate layer 11 with the comdenstion water from the water tank 21 pump through setting up second pump 202 in, improve heat exchange efficiency, in time take away the heat that changes the membrane groove 13 and produce.
Preferably, the device further comprises a cover: the inner shell 12 is open-ended with a cover. Can prevent foreign matters from falling into the film rotating groove 13 to pollute the experimental sample.
Preferably, a layer of thermal insulation material is provided on the outer wall of the water tank 21. The temperature of the condensed water in the box body is kept, the temperature is prevented from rising, and the solar water heater is particularly suitable for being used in summer.
Preferably, the method comprises the following steps: the temperature sensor 131 and the display, the temperature sensor 131 is arranged on the inner side wall of the inner shell 12, and is arranged opposite to the condensed water outlet on the outer shell 10; the display is disposed on the outer wall of the housing 10 and electrically connected to the temperature sensor 131. Through setting up temperature sensor 131, can show the temperature in changeing the membrane groove 13 on shell 10 in real time, the experimenter of being convenient for changes and acquires the temperature condition in changeing the membrane groove 13 to control the circulating speed of comdenstion water according to this, realize the accurate control to commentaries on classics membrane liquid temperature.
Reference throughout this specification to "one embodiment," "another embodiment," "an embodiment," "a preferred embodiment," or the like, means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally in this application. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the disclosure to effect such feature, structure, or characteristic in connection with other embodiments.
Although the present application has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure and claims of this application. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (10)
1. A refrigeration rotary film tank, comprising: the film-rotating device comprises an outer shell (10), an inner shell (12), a water tank (21) and a semiconductor refrigerator (22), wherein a film-rotating groove (13) is arranged in the inner shell (12), and a closed interlayer (11) is arranged between the inner shell (12) and the outer shell (10);
the top of the inner shell (12) is higher than the top surface of the outer shell (10), and the top surface of the inner shell (12) is open and extends out of the outer shell (10);
the water outlet of the interlayer (11) is communicated with the water inlet pipeline of the water tank (21);
the water outlet of the water tank (21) is communicated with the water inlet pipeline of the interlayer (11);
the semiconductor refrigerator (22) is arranged on the outer wall of the water tank (21);
the refrigeration aluminum block (221) of the semiconductor refrigerator (22) extends into the water tank (21).
2. The refrigeration film transferring tank as claimed in claim 1, wherein the water outlet of the interlayer (11) is arranged on the upper side wall of the shell (10);
the water inlet of the water tank (21) is arranged on the side wall of the upper part of the water tank (21).
3. The refrigeration film transferring tank is characterized by comprising a first pipeline, wherein the first pipeline is arranged on the side wall of the shell (10), and the first pipeline is communicated with a water outlet of the interlayer (11) and a water inlet of the water tank (21).
4. The refrigeration wiped film tank of claim 3, wherein a first pump (201) is disposed on the first pipeline.
5. The refrigeration film transfer tank as recited in claim 1, characterized in that the water inlet of the interlayer (11) is arranged on the lower side wall of the shell (10);
the water outlet of the water tank (21) is arranged on the side wall of the lower part of the water tank (21).
6. The refrigeration wiped film tank of claim 5, comprising: the side wall of the shell (10) is provided with a second pipeline, and the first end of the second pipeline is communicated with the water inlet of the interlayer (11);
the second end of the second pipeline is communicated with the water outlet of the water tank (21).
7. The refrigerated wiped film transfer tank of claim 6 wherein a second pump (202) is provided on the second line.
8. The refrigeration wiped film tank of claim 1, further comprising: and the opening of the inner shell (12) is provided with a cover.
9. The refrigeration wiped film tank of claim 1, comprising: the temperature sensor (131) is arranged on the inner side wall of the inner shell (12) and is opposite to a condensed water outlet on the outer shell (10);
the display is arranged on the outer wall of the shell (10) and is electrically connected with the temperature sensor (131).
10. A refrigerating rotary film tank according to claim 1, characterized in that a layer of heat insulating material is arranged on the outer wall of the water tank (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020960525.0U CN212228963U (en) | 2020-05-29 | 2020-05-29 | Refrigeration film-transferring groove |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020960525.0U CN212228963U (en) | 2020-05-29 | 2020-05-29 | Refrigeration film-transferring groove |
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| Publication Number | Publication Date |
|---|---|
| CN212228963U true CN212228963U (en) | 2020-12-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020960525.0U Expired - Fee Related CN212228963U (en) | 2020-05-29 | 2020-05-29 | Refrigeration film-transferring groove |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113624960A (en) * | 2021-07-22 | 2021-11-09 | 华中科技大学同济医学院附属协和医院 | Low-temperature constant-temperature film transfer device |
-
2020
- 2020-05-29 CN CN202020960525.0U patent/CN212228963U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113624960A (en) * | 2021-07-22 | 2021-11-09 | 华中科技大学同济医学院附属协和医院 | Low-temperature constant-temperature film transfer device |
| CN113624960B (en) * | 2021-07-22 | 2023-08-08 | 华中科技大学同济医学院附属协和医院 | Low-temperature constant-temperature film transferring device |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201225 |
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| CF01 | Termination of patent right due to non-payment of annual fee |