CN103735634B - There is Chinese medicine composition of blackening and nourishing hair effect and preparation method thereof - Google Patents
There is Chinese medicine composition of blackening and nourishing hair effect and preparation method thereof Download PDFInfo
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Abstract
The invention discloses and a kind ofly have Chinese medicine composition of blackening and nourishing hair effect and preparation method thereof, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 1-40 part, edge strip ginseng 1-40 part, Radix Notoginseng 1-40 part, Flos Carthami 1-30 part, Radix Polygoni Multiflori 1-30 part, Radix Angelicae Sinensis 1-30 part, beautiful Herba Cistanches 1-30 part and Cortex Sapii Radicis 1-30 part; Preparation method: Radix Polygoni Multiflori supercritical CO
2extraction, adds 45-55% alcoholic solution, obtains Radix Polygoni Multiflori living solution; Pulverized by all the other raw material dryings, add 45-55% alcoholic solution, vacuum decompression refluxes, and through ceramic composite membrane microfiltration equipment and filter-cloth filtering, obtains clear liquor; Radix Polygoni Multiflori living solution and clear liquor are mixed.Chinese medicine composition tonification of can dispeling the wind is invigorated blood circulation, and promotes scalp blood circulation, makes scalp, hair and hair follicle obtain sufficient nutrition, promotes that the division of root of hair melanocyte, activity of tyrosinase and melanin generate, realizes blackening and nourishing hair effect from root.
Description
Technical field
The present invention relates to the field of Chinese medicines, particularly relate to and a kind of there is Chinese medicine composition of blackening and nourishing hair effect and preparation method thereof.
Background technology
Along with the development of society, the quickening of working and learning rhythm, the mental pressure that people bear increases the weight of day by day, increasing people perplex by poliosis.At all times large quantifier elimination is carried out for changing white hair into black hair, but also there is no completely successfully way and medicament scheme, poor effect.Because poliosis has a strong impact on U.S. of human body image and face, a lot of white originator is constantly had hair dyed in helpless and disappointment, but most hair dye all contains p-phenylenediamine (PPD), p-phenylenediamine (PPD) is the effective ingredient in hair dye, to health, there is potential hazard, this material can cause skin allergy, coming in contact property or allergic dermatitis.In addition, p-phenylenediamine (PPD) is internationally recognized a kind of carcinogen, has the risk increasing the cancers such as bladder cancer.
Therefore, a kind of safety product that can make changing white hair into black hair is needed badly.
Summary of the invention
Based on this, the object of the invention is that providing a kind of has Chinese medicine composition of blackening and nourishing hair effect and preparation method thereof.
The technical scheme solved the problems of the technologies described above is as follows:
Have a Chinese medicine composition for blackening and nourishing hair effect, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 1-40 part, edge strip ginseng 1-40 part, Radix Notoginseng 1-40 part, Flos Carthami 1-30 part, Radix Polygoni Multiflori 1-30 part, Radix Angelicae Sinensis 1-30 part, beautiful Herba Cistanches 1-30 part and Cortex Sapii Radicis 1-30 part.
Wherein in some embodiments, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 10-20 part, edge strip ginseng 10-20 part, Radix Notoginseng 5-10 part, Flos Carthami 10-20 part, Radix Polygoni Multiflori 15-20 part, Radix Angelicae Sinensis 10-20 part, beautiful Herba Cistanches 10-20 part and Cortex Sapii Radicis 15-20 part.
Wherein in some embodiments, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 15 parts, edge strip join 20 parts, 5 parts, Radix Notoginseng, 15 parts, Flos Carthami, Radix Polygoni Multiflori 20 parts, Radix Angelicae Sinensis 10 parts, beautiful Herba Cistanches 15 parts, Cortex Sapii Radicis 20 parts.
The present invention also provides a kind of preparation method with the Chinese medicine composition of blackening and nourishing hair effect, comprises the following steps:
(1) by above-mentioned weight portion, Radix Polygoni Multiflori be dry, pulverize, carry out supercritical CO
2extraction, obtains Radix Polygoni Multiflori active matter; Described supercritical CO
2the technological parameter of extraction is: the extraction time in extraction kettle is 5-6h, and the pressure of extraction kettle is 20-22Mpa, and entrainer ethanol accounts for extractant CO
2percentage by weight be 11-13%; The pressure of separating still is 5-8Mpa, and temperature is 30-35 DEG C, CO
2flow be 20-22kg/h;
(2) in the Radix Polygoni Multiflori active matter of step (1) gained, add the weight of ethanol water to the former medicine of Radix Polygoni Multiflori that volume fraction is 45-55%, obtain Radix Polygoni Multiflori living solution;
(3) by above-mentioned weight portion, by Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis, dry, mixing, pulverizes, obtains Traditional Chinese drug mixture, the volume fraction adding 10-15 times of weight by the weight of Traditional Chinese drug mixture is the ethanol water of 45-55%, after merceration, under 80-90 DEG C of condition, extract in vacuum decompression return tank, obtain extracting solution, by extracting solution through Ceramic Composite membrane filtration, obtain filtrate, by filtrate cryopreservation after 2-3 days, filter-cloth filtering, obtains clear liquor;
(4) clear liquor of the Radix Polygoni Multiflori living solution of step (2) gained and step (3) gained is mixed.
Wherein in some embodiments, the pore footpath of described ceramic composite membrane is 100-200nm, and the aperture of described filter cloth is 400-1000 order.
Wherein in some embodiments, the pore footpath of described ceramic composite membrane is 100nm, and the aperture of described filter cloth is 1000 orders.
Wherein in some embodiments, the particle diameter pulverized described in step (1) and step (3) is more than 40 orders.
Wherein in some embodiments, the time be separated in separating still in step (1) is 3-5h.
Wherein in some embodiments, the time of merceration described in step (3) is 1-2h.
Wherein in some embodiments, described in step (3), low temperature is 4-6 DEG C.
In Chinese medicine composition of the present invention, the drug effect of each raw material is described as follows:
Radix Panacis Quinquefolii: have another name called Radix Panacis Quinquefolii, Araliaceae, originates in America, has plantation in China Three Eastern Provinces, Fujian, Yunnan.Radix Panacis Quinquefolii sweet and slightly bitter taste is cool." property of medicine is examined ": " nourishing YIN is brought down a fever.Processed with Rhizoma Zingiberis Recens QI invigorating, righting gas "." Records of Tradition Chinese and Western Medicine in Combination ": " can edema caused by disorder of QI be subsidized, and energy tonification blood system ".
Edge strip is joined: the one of Radix Ginseng, and gas is fragrant, and mildly bitter flavor, returns spleen, lung, heart channel.Be Chinese tradition rare Chinese medicine, there is invigorating the spleen to benefit the lung, strongly invigorating primordial QI, the heart strengthening and the mind tranquilizing, promoting the production of body fluid to quench thirst, answer the effects such as arteries and veins takes off admittedly.Compendium of Material Medica is thought and can be treated " all deficient syndromes of male woman ".
Radix Notoginseng: have another name called Radix Notoginseng, belongs to Araliaceae Panax herbaceous plant for many years, the peculiar rare Chinese medicine of China.Sweet in the mouth, micro-hardship, warm in nature, return Liver Channel, stomach warp.Record in Qing Dynasty's pharmaceutical texts supplementary Amplifications of the Compendium of Materia Medica: " ginseng qi-tonifying the first, Radix Notoginseng enriches blood the first, taste with and merit also etc., therefore claiming Panax pseudoginseng, is the most precious person in Chinese medicine.
Flos Carthami: Compositae Flos Carthami platymiscium, effect mainly promoting blood circulation to restore menstrual flow of Flos Carthami, for blood stasis amenorrhea.
Radix Polygoni Multiflori: Polygonaceae Radix Polygoni Multiflori platymiscium, bitter in the mouth, sweet; Puckery, slightly warm in nature.Return liver, kidney channel." book on Chinese herbal medicine is looked for the truth " is called its " grow water the kidney invigorating, hair color is made light of one's life by commiting suicide ".
Radix Angelicae Sinensis: Umbelliferae archangel, sweet in the mouth, pungent, bitter, warm in nature, return liver, the heart, spleen channel." Japan hanako materia medica " carries its " control a wind cutting, all blood, mend all labor, broken stagnant blood, supports fresh blood ".
Beautiful Herba Cistanches: Orobanchaceae Cistanche Hoffmgg. et Link plants, stem is used as medicine (Chinese medicine name: Herba Cistanches), makes a living and rue greatly after adopting rear airing, and salting is that salt rues greatly, has the title of " desert Radix Ginseng ", have effect of replenishing vital essence and blood, kidney-reinforcing Yang-strengthening, loosening bowel to relieve constipation in the Northwest.
Cortex Sapii Radicis: Euphorbiaceae sapium deciduous tree, property is bitter, and tepor, enters lung, spleen, kidney, large intestine channel.Parasite killing is had to detoxify, effect of diuresis relieving constipation.Furuncle is controlled in external, clavus, mastitis, traumatic injury, eczema, dermatitis.
Of the present invention a kind ofly have Chinese medicine composition of blackening and nourishing hair effect and preparation method thereof and have the following advantages and beneficial effect:
(1) Chinese medicine composition of the present invention is through a large amount of experiment of inventor and research, selected Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Polygoni Multiflori, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis, and draw its optimum formula component, this kind of compositions safety non-toxic obtained, and have dispel the wind, tonification, the effect of invigorating blood circulation, and many medicines share, jointly can play and promote scalp blood circulation, scalp, hair and hair follicle is made to obtain sufficient nutrition, can promote that the division of root of hair melanocyte, activity of tyrosinase and melanin generate simultaneously, realize effect of blackening and nourishing hair from root.
(2) preparation method of Chinese medicine composition of the present invention adopts supercritical CO
2extraction equipment and ceramic composite membrane microfiltration equipment obtain effective ingredient, and the efficiency of this kind of method extraction effective ingredient is high, and technique is simple to operation, is applicable to large-scale production.
(3) pass through in preparation method of the present invention strictly to control supercritical CO
2pressure and temperature in extraction equipment, and in conjunction with extract at room temperature, effectively can prevent oxidation and the loss of heat-sensitive materials, ceramic composite membrane microfiltration equipment can the macromolecular substances such as effectively catching protein, fat, and when avoiding product to place for a long time, the association of macromolecular substances produces precipitation.
Accompanying drawing explanation
Fig. 1 is the measurement result figure of experimental group and cellular control unit propagation in embodiment 8;
Fig. 2 is experimental group and matched group tyrosinase activity measurement result figure in embodiment 8;
Fig. 3 is experimental group and matched group melanin content measurement result figure in embodiment 8.
Detailed description of the invention
In the present invention, cited plant material, wherein Section Plant Material specifically utilizes position for raw material with it, is specially: root, the flower of Flos Carthami, the root of Radix Notoginseng, the root of Radix Angelicae Sinensis, the stem of beautiful Herba Cistanches, the bark of Cortex Sapii Radicis of the tuber of Radix Polygoni Multiflori, the root of Radix Panacis Quinquefolii, edge strip ginseng.
Below with reference to specific embodiment, the present invention will be further described.
Embodiment 1
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 20g, Radix Panacis Quinquefolii 15g, edge strip ginseng 20g, Radix Notoginseng 5g, Flos Carthami 15g, Radix Angelicae Sinensis 10g, beautiful Herba Cistanches 15g, Cortex Sapii Radicis 20g;
The preparation method of above-mentioned Chinese medicine composition, comprises the steps:
(1) take Radix Polygoni Multiflori 20g, dry, pulverize to 40 orders, the Radix Polygoni Multiflori powder of pulverizing is placed in extraction kettle and is immersed in CO
25 hours are extracted, CO in liquid
2in soak, entrainer ethanol weight is extractant CO
212% of weight, controlling pressure in extraction kettle is 20Mpa; Be separated in separating still after immersion, controlling disjoining pressure force value in separating still is 5Mpa, heats to 30 DEG C, keeps 3 hours, CO
2flow maintain 20Kg/h; Separating obtained Radix Polygoni Multiflori extract is Radix Polygoni Multiflori active component;
(2) Radix Polygoni Multiflori extract obtained for step (1) is added volume fraction be 50% ethanol water ad pond om be 20g, stirring and dissolving, obtained Radix Polygoni Multiflori living solution;
(3) Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis mixing is taken by above-mentioned weight, dry, be crushed to more than 40 orders, obtain mixture, by the weight of mixture, add 50% ethanol water of 1000g, merceration is after 1 hour, heating is under the condition of 80 DEG C in temperature, extracts 1 hour with in vacuum decompression return tank; By extracting solution by ceramic composite membrane microfiltration equipment, wherein ceramic compound film tube aperture is 100nm; Extracting solution is cooled to 5 DEG C, preserves 2 days, then be clear liquor with 1000 order filter-cloth filterings;
(4) the Radix Polygoni Multiflori living solution that step (2) is obtained is joined in the obtained clear liquor of step (3), stir.
Using method: clean hair post processing dry to seventy percent, get appropriate this product and rub alopecia root, then massage 3-5 minute gently, with horn comb combing.
Embodiment 2
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 20g, Radix Panacis Quinquefolii 20g, edge strip ginseng 10g, Radix Notoginseng 10g, Flos Carthami 10g, Radix Angelicae Sinensis 10g, beautiful Herba Cistanches 20g, Cortex Sapii Radicis 15g;
The preparation method of above-mentioned Chinese medicine composition, comprises the steps:
(1) Radix Polygoni Multiflori extract is the preparation method of Radix Polygoni Multiflori active component with reference to embodiment 1 step (1);
(2) preparation method of Radix Polygoni Multiflori living solution is with reference to embodiment 1 step (2);
(3) Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis mixing is taken by above-mentioned weight, dry, be crushed to more than 40 orders, obtain mixture, by the weight of mixture, add 50% ethanol of 950g, merceration is after 1 hour, heating is under the condition of 80 DEG C in temperature, extracts 1 hour with in vacuum decompression return tank; By extracting solution by ceramic composite membrane microfiltration equipment, wherein ceramic compound film tube aperture is 100nm; Extracting solution is cooled to 5 DEG C, preserves 2 days, then be supernatant liquid with 1000 order filter-cloth filterings;
(4) the Radix Polygoni Multiflori living solution that step (2) is obtained is joined in the obtained clear liquor of step (3), stir.
Using method: with reference to using method described in embodiment 1.
Embodiment 3
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 1g, Radix Panacis Quinquefolii 1g, edge strip ginseng 40g, Radix Notoginseng 1g, Flos Carthami 30g, Radix Angelicae Sinensis 30g, beautiful Herba Cistanches 1g, Cortex Sapii Radicis 30g;
The preparation method of above-mentioned Chinese medicine composition, comprises the steps:
(1) the Radix Polygoni Multiflori living solution 1g that Example 1 step (2) is obtained;
(2) Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis mixing is taken by above-mentioned weight, dry, be crushed to more than 40 orders, obtain mixture, by the weight of mixture, add the 50% ethanol merceration of 1995g after 2 hours, heating, be under the condition of 90 DEG C in temperature, extract 2 hours with in vacuum decompression return tank; By extracting solution by ceramic composite membrane microfiltration equipment, wherein ceramic compound film tube aperture is 100nm; Extracting solution is cooled to 5 DEG C, preserves 3 days, then be clear liquor with 1000 order filter-cloth filterings;
(3) step (1) described 1g Radix Polygoni Multiflori living solution is joined in the obtained clear liquor of step (2), stir.
Using method: with reference to using method described in embodiment 1.
Embodiment 4
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 15g, Radix Panacis Quinquefolii 10g, edge strip ginseng 20g, Radix Notoginseng 5g, Flos Carthami 20g, Radix Angelicae Sinensis 20g, beautiful Herba Cistanches 10g, Cortex Sapii Radicis 20g;
The preparation method of above-mentioned Chinese medicine composition, comprises the steps:
(1) taking Radix Polygoni Multiflori dry, pulverize to 40 orders, the Radix Polygoni Multiflori powder of pulverizing is placed in extraction kettle and is immersed in CO
26 hours are extracted, CO in liquid
2in soak, entrainer ethanol weight is extractant CO
211% of weight, controlling pressure in extraction kettle is 21Mpa; Be separated in separating still after immersion, controlling disjoining pressure force value in separating still is 6Mpa, heats to 32 DEG C, keeps 4 hours, CO
2flow maintain 21kg/h; Separating obtained Radix Polygoni Multiflori extract is Radix Polygoni Multiflori active component;
(2) Radix Polygoni Multiflori extract obtained for step (1) is added volume fraction be 45% ethanol water ad pond om be 15g, stirring and dissolving, obtained Radix Polygoni Multiflori living solution;
(3) Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis mixing is taken by above-mentioned weight, dry, be crushed to more than 40 orders, obtain mixture, by the weight of mixture, add the 45% ethanol merceration of 1320g after 1.5 hours, heating, be under the condition of 87 DEG C in temperature, extract 1.5 hours with in vacuum decompression return tank; By extracting solution by ceramic composite membrane microfiltration equipment, wherein ceramic compound film tube aperture is 200nm; Extracting solution is cooled to 4 DEG C, preserves 2.5 days, then be clear liquor with 400 order filter-cloth filterings;
(4) the Radix Polygoni Multiflori living solution that step (2) is obtained is joined in the obtained clear liquor of step (3), stir.
Using method: with reference to using method described in embodiment 1.
Embodiment 5
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 15g, Radix Panacis Quinquefolii 15g, edge strip ginseng 15g, Radix Notoginseng 7g, Flos Carthami 15g, Radix Angelicae Sinensis 15g, beautiful Herba Cistanches 15g, Cortex Sapii Radicis 17g;
The preparation method of above-mentioned Chinese medicine composition, comprises the steps:
(1) taking Radix Polygoni Multiflori dry, pulverize to 40 orders, the Radix Polygoni Multiflori powder of pulverizing is placed in extraction kettle and is immersed in CO
26 hours are extracted, CO in liquid
2in soak, entrainer ethanol weight is 13% of extractant CO2 weight, and controlling pressure in extraction kettle is 22Mpa; Be separated in separating still after immersion, controlling disjoining pressure force value in separating still is 8Mpa, heats to 35 DEG C, keeps 5 hours, CO
2flow maintain 22kg/h; Separating obtained Radix Polygoni Multiflori extract is Radix Polygoni Multiflori active component;
(2) Radix Polygoni Multiflori extract obtained for step (1) is added volume fraction be 55% ethanol water ad pond om be 15g, stirring and dissolving, obtained Radix Polygoni Multiflori living solution;
(3) Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis mixing is taken by above-mentioned weight, dry, be crushed to more than 40 orders, obtain mixture, by the weight of mixture, give in mixing medicinal powder and add the 55% ethanol merceration of 1390g after 1 hour, heating, be under the condition of 90 DEG C in temperature, extract 2 hours with in vacuum decompression return tank; By extracting solution by ceramic composite membrane microfiltration equipment, wherein ceramic compound film tube aperture is 100nm; Extracting solution is cooled to 6 DEG C, preserves 2 days, then be clear liquor with 1000 order filter-cloth filterings;
(4) the Radix Polygoni Multiflori living solution that step (2) is obtained is joined in the obtained clear liquor of step (3), stir.
Using method: with reference to using method described in embodiment 1.
Embodiment 6
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 10g, Radix Panacis Quinquefolii 10g, edge strip ginseng 10g, Radix Notoginseng 10g, Flos Carthami 10g, Radix Angelicae Sinensis 10g, beautiful Herba Cistanches 10g, Cortex Sapii Radicis 10g;
The preparation method of above-mentioned Chinese medicine composition is concrete with reference to embodiment 4.
Embodiment 7
There is a Chinese medicine composition for blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 30g, Radix Panacis Quinquefolii 40g, edge strip ginseng 40g, Radix Notoginseng 40g, Flos Carthami 30g, Radix Angelicae Sinensis 30g, beautiful Herba Cistanches 30g, Cortex Sapii Radicis 30g;
The preparation method of above-mentioned Chinese medicine composition is concrete with reference to embodiment 4.
Embodiment 8 evaluates blackening and nourishing hair effect of Chinese medicine composition
One, experiment purpose
By the Chinese medicine composition obtained by mensuration embodiment 1-7 to B16F1 Melanoma Cell Proliferation, the impact of tyrosinase activity and B16 cell, evaluates its blackening and nourishing hair effect.
Two, experimental technique
1, experiment material
Mouse black-in lymphoma B16F1 cell (Wuhan University's Type Tissue Collection), RPMI-1640 culture medium (purchased from American Gibico company), DMEM high glucose medium (purchased from American Gibico company), hyclone FBS(is purchased from Hyclone company), HEPES(is purchased from Promega company), trypsin Typsin) (purchased from Ameresco company), L-3,4 dihydroxyphenylalanine (purchased from American Sigma company), TritonX-100(purchased from American Sigma company), penicillin, streptomycin sulfate, dimethyl sulfoxide, NaOH(analytical pure), MTT(tetrazolium bromide) etc. reagent.
2, cell culture and medicine irritation
2.1 groupings:---embodiment 1-7 and matched group---comparative example 1 that is divided into experimental group;
Wherein, there is described in above-mentioned comparative example 1 Chinese medicine composition of blackening and nourishing hair effect, be prepared from by following raw material:
Radix Polygoni Multiflori 0.1g, Radix Panacis Quinquefolii 50g, edge strip ginseng 0.1g, Radix Notoginseng 50g, Flos Carthami 0.2g, Radix Angelicae Sinensis 5g, beautiful Herba Cistanches 25g, Cortex Sapii Radicis 5g;
The preparation method of above-mentioned comparative example 1 Chinese medicine composition, comprises the steps:
(1) the Radix Polygoni Multiflori living solution 0.1g that Example 1 step (2) is obtained;
(2) Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, beautiful Herba Cistanches and Cortex Sapii Radicis mixing is taken by above-mentioned weight, dry, be crushed to more than 40 orders, obtain mixture, by the weight of mixture, give in mixing medicinal powder and add the 50% ethanol merceration of 2031g after 2 hours, heating, be under the condition of 80 DEG C in temperature, extract 1 hour with in vacuum decompression return tank; By extracting solution by ceramic composite membrane microfiltration equipment, wherein ceramic compound film tube aperture is 100nm; Extracting solution is cooled to 5 DEG C, preserves 2 days, then be clear liquor with 1000 order filter-cloth filterings;
(3) step (1) described 0.1g Radix Polygoni Multiflori living solution is joined in the obtained clear liquor of step (2), stir.
2.2 cell culture
With DMEM culture medium 37 DEG C, 5%CO containing 10% hyclone
2cellar culture mouse black-in lymphoma B16F1 cell under condition, after going down to posterity 24 hours, carries out dosing stimulation with the Chinese medicine composition that experimental group and matched group obtain respectively to cell, and experimentally requires agent-feeding treatment reasonable time, specifically see 2.3-2.5.
The mensuration of 2.3 cell proliferation
The concentration of passage cell is adjusted to 1 × 10
5individual/mL adds 96 well culture plates, every hole 0.1mL.Hatch 24 hours, treat that cell attachment is thick, suck old culture fluid, add the fresh medium 0.1mL of embodiment 1-7 and the Chinese medicine composition obtained by comparative example 1, be placed in 37 DEG C, 5%CO
2continue cultivation under condition 48 hours, adopt classical MTT colorimetry (concrete grammar can see Ma Huijun, red legend unit, Wang great Guang, etc.Chinese medicine monomer piperine promotes the experimentation of epidermis melanoma cells melanin genesis, clinic skin section magazine, 2005,34(1): 14-17) measure cell proliferation rate.Each embodiment sets up 3 multiple holes, averages, and to arrange culture medium be blank.
Cell proliferation rate (%)=each concentration mean absorbance values/blank group mean absorbance values × 100%.
2.4 tyrosinase activities measure
Get 0.1mL embodiment 1-7 and the Chinese medicine composition effects Mice melanoma b16 F1 cell obtained by comparative example 1 after 2 days, measure tyrosinase activity (specifically see Ma Huijun, red legend unit, Yue Xuezhuan, etc.Methoxsalen on the research affecting intracellular signaling of cultivating people epidermis melanoma cells melanin genesis, clinic skin section magazine, 2004, (33): 526-528).Each embodiment sets up 3 multiple holes, averages, and to arrange culture medium be blank.
Activity ratio (%)=each embodiment mean absorbance values/blank group mean absorbance values × 100% of tryrosinase.
2.5 melanin contents measure
By the cell suspension of configuration with 1 × 10
5individual/mL density is inoculated in 6 orifice plates, add 0.1mL embodiment 1-7 and the Chinese medicine composition obtained by comparative example 1 and continue cultivation 2 days after hatching 1 day, measure melanin content (specifically see Jiang Zequn, Wu Qiong, Xu Jimin, what light source, etc.Chinese medicine Radix Polygoni Multiflori promotes the Study on mechanism that melanin generates, Nanjing University of Traditional Chinese Medicine's journal, 2010, (26): 190-192).Each embodiment sets up 3 multiple holes, averages, and to arrange culture medium be blank.
Melanin content (%)=each embodiment A
400value/blank group A
400value × 100%.
Three, experimental result
The measurement result of cell proliferation, refers to Fig. 1; As can be seen from Figure 1: compared with the Chinese medicine composition obtained by comparative example 1, the Chinese medicine composition prepared by embodiment 1-7 has facilitation to mice melanoma cells, and the successful of embodiment 1-7 is better than comparative example 1; Embodiment 7 illustrates that compared with embodiment 1-6 continuing to add raw material after the interpolation weight portion of each raw material of Chinese medicine composition acquires a certain degree does not have facilitation to product efficacy.
Tyrosinase activity measurement result, refers to Fig. 2; As can be seen from Figure 2: compared with the Chinese medicine composition obtained by comparative example 1, the Chinese medicine composition prepared by embodiment 1-7 obviously can promote the activity of B16F1 mouse melanin tumor cell tryrosinase, and its successful is better than comparative example 1; Embodiment 7 illustrates that compared with embodiment 1-6 continuing to add raw material after the interpolation weight portion of each raw material of Chinese medicine composition acquires a certain degree does not have facilitation to product efficacy.
Melanin content measurement result, refers to Fig. 3; As can be seen from Figure 3: compared with the Chinese medicine composition obtained by comparative example 1, the Chinese medicine composition prepared by embodiment 1-7 can obviously promote increasing of melanin content, and successful is better than comparative example 1.Embodiment 7 illustrates that compared with embodiment 1-6 continuing to add raw material after the interpolation weight portion of each raw material of Chinese medicine composition acquires a certain degree does not have facilitation to product efficacy.
Embodiment 9 stability test
One, experiment purpose
The stability of the Chinese medicine composition obtained by Evaluation operation example 1.
Two, experimental technique
By formula and method described in embodiment 1, obtained Chinese medicine composition 600g, is respectively charged in the transparent test bottle of 6 100g capacity, does stability test in (20 ± 1) DEG C, (45 ± 1) DEG C, (5 ± 1) DEG C.
Three, experimental result
Result is see table 1.
Table 1 estimation of stability result table
The Chinese medicine composition of embodiment 1 maintains good stability in the heat-resisting cold-resistant stability test of routine as can be seen from Table 1, does not have the unusual conditions such as Precipitation, variable color, muddiness, shows that Chinese medicine composition of the present invention has good stability.
Embodiment 10
One, experiment purpose
Hair color effect in Evaluation operation example 1 practical application.
Two, experimental technique
Use through 20 poliosis patients with the Chinese medicine composition obtained by embodiment 1, carried out long-term observation.In 20 routine patients, male 10 people, female 10 people, minimum 26 years old of age, maximum 45 years old, 35 years old mean age.Every day 1 time, treatment time is 1 year.
Using method: clean hair post processing dry to seventy percent, get appropriate this product and rub alopecia root, then massage 3-5 minute gently, with horn comb combing.
Efficacy evaluation: the whole blackening of poliosis is recovery from illness, and poliosis blackening is greater than 80% for effective, and it is effective that poliosis blackening is greater than 30%, and before and after treatment change, change is greatly not invalid.
Three, experimental result
20 routine patients use the Chinese medicine composition of embodiment 1 to treat, have received satisfied curative effect, wherein recovery from illness 6 example, effective 10 examples, effective 1 example, invalid 3 examples, treat total obvious effective rate and reach 80%, illustrate that the Chinese medicine composition obtained by embodiment 1 has good hair color effect, also can show that Chinese medicine composition of the present invention has good hair color effect simultaneously.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (9)
1. one kind has the Chinese medicine composition of blackening and nourishing hair effect, it is characterized in that, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 1-40 part, edge strip ginseng 1-40 part, Radix Notoginseng 1-40 part, Flos Carthami 1-30 part, Radix Polygoni Multiflori 1-30 part, Radix Angelicae Sinensis 1-30 part, Herba Cistanches 1-30 part and Cortex Sapii Radicis 1-30 part; The preparation method of described Chinese medicine composition comprises the following steps:
(1) by described weight portion, Radix Polygoni Multiflori be dry, pulverize, carry out supercritical CO
2extraction, obtains Radix Polygoni Multiflori active matter; Described supercritical CO
2the technological parameter of extraction is: the extraction time in extraction kettle is 5-6h, and the pressure of extraction kettle is 20-22Mpa, and entrainer ethanol accounts for extractant CO
2percentage by weight be 11-13%; The pressure of separating still is 5-8Mpa, and temperature is 30-35 DEG C, CO
2flow be 20-22kg/h;
(2) in the Radix Polygoni Multiflori active matter of step (1) gained, add the weight of ethanol water to the former medicine of Radix Polygoni Multiflori that volume fraction is 45-55%, obtain Radix Polygoni Multiflori living solution;
(3) by described weight portion, by Radix Panacis Quinquefolii, edge strip ginseng, Radix Notoginseng, Flos Carthami, Radix Angelicae Sinensis, Herba Cistanches and Cortex Sapii Radicis, dry, mixing, pulverize, obtain Traditional Chinese drug mixture, the volume fraction adding 10-15 times of weight by the weight of Traditional Chinese drug mixture is the ethanol water of 45-55%, after merceration, under 80-90 DEG C of condition, extract in vacuum decompression return tank, obtain extracting solution, extracting solution is filtered through ceramic composite membrane microfiltration equipment, obtain filtrate, again by filtrate cryopreservation after 2-3 days, filter-cloth filtering, obtains clear liquor;
(4) clear liquor of the Radix Polygoni Multiflori living solution of step (2) gained and step (3) gained is mixed, there is described in namely obtaining the Chinese medicine composition of blackening and nourishing hair effect.
2. a kind of Chinese medicine composition with blackening and nourishing hair effect according to claim 1, it is characterized in that, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 10-20 part, edge strip ginseng 10-20 part, Radix Notoginseng 5-10 part, Flos Carthami 10-20 part, Radix Polygoni Multiflori 15-20 part, Radix Angelicae Sinensis 10-20 part, Herba Cistanches 10-20 part and Cortex Sapii Radicis 15-20 part.
3. a kind of Chinese medicine composition with blackening and nourishing hair effect according to claim 2, it is characterized in that, described Chinese medicine composition is prepared from by the raw material of following weight parts: Radix Panacis Quinquefolii 15 parts, edge strip join 20 parts, Radix Notoginseng 5 parts, 15 parts, Flos Carthami, Radix Polygoni Multiflori 20 parts, Radix Angelicae Sinensis 10 parts, Herba Cistanches 15 parts, Cortex Sapii Radicis 20 parts.
4. the Chinese medicine composition with blackening and nourishing hair effect according to claim 1, is characterized in that, the pore footpath of step (3) described ceramic composite membrane is 100-200nm, and the aperture of described filter cloth is 400-1000 order.
5. the Chinese medicine composition with blackening and nourishing hair effect according to claim 4, is characterized in that, the pore footpath of described ceramic composite membrane is 100nm, and the aperture of described filter cloth is 1000 orders.
6. the Chinese medicine composition with blackening and nourishing hair effect according to claim 1, is characterized in that, the particle diameter pulverized described in step (1) and step (3) is more than 40 orders.
7. the Chinese medicine composition with blackening and nourishing hair effect according to claim 1, is characterized in that, the time be separated in separating still in step (1) is 3-5h.
8. the Chinese medicine composition with blackening and nourishing hair effect according to claim 1, is characterized in that, the time of merceration described in step (3) is 1-2h.
9. the Chinese medicine composition with blackening and nourishing hair effect according to claim 1, is characterized in that, described in step (3), low temperature is 4-6 DEG C.
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| CN106691949A (en) * | 2017-02-27 | 2017-05-24 | 广东黑霸生物科技有限公司 | Traditional Chinese medicine extract liquid having hair blackening effect, and preparation method and application thereof |
| CN106821856A (en) * | 2017-03-14 | 2017-06-13 | 佛山文森特知识产权服务有限公司 | A kind of seven black hair blackening cosmetic compositions and preparation method thereof |
| CN108714178A (en) * | 2018-06-01 | 2018-10-30 | 广州发元生物科技有限公司 | Chinese medicine composition and preparation method thereof with blackening and nourishing hair effect |
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| EP1504763A1 (en) * | 2002-05-14 | 2005-02-09 | ZHU, Jianzhong | Traditional chinese pharmaceutical formulation for treatment of paradentosis, process for preparation and use thereof |
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| EP1504763A1 (en) * | 2002-05-14 | 2005-02-09 | ZHU, Jianzhong | Traditional chinese pharmaceutical formulation for treatment of paradentosis, process for preparation and use thereof |
| CN101284073A (en) * | 2007-04-13 | 2008-10-15 | 雷泽红 | Combination medicine for curing the hoary hair |
| CN102018888A (en) * | 2010-12-08 | 2011-04-20 | 樊哲京 | Cream for promoting hair growth and blackening hair and preparation method thereof |
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