CN103691156A - Antiskid chromatographic column - Google Patents
Antiskid chromatographic column Download PDFInfo
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- CN103691156A CN103691156A CN201310644722.6A CN201310644722A CN103691156A CN 103691156 A CN103691156 A CN 103691156A CN 201310644722 A CN201310644722 A CN 201310644722A CN 103691156 A CN103691156 A CN 103691156A
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Abstract
The invention provides an antiskid chromatographic column, belonging to the field of biological purification instruments. According to the chromatographic column provided by the invention, the top end of the column body is provided with a U-shaped screen, so that a buffer solution, a sample solution, an eluate and other liquids can conveniently flow down along the wall of the column body, thereby avoiding splashing and influencing the chromatographic effect; and the external surface of the column is provided with an antiskid plastic outer sleeve, so that the column body can be held by hands or fixed to a bracket, and the chromatographic column can not be crashed easily, thereby preventing the clip from leaving scratches on the column body made of glass. The chromatographic column has the advantages of simple structure and favorable protein purification effect, and is convenient to operate.
Description
Technical field
The invention belongs to biologically pure apparatus, be specifically related to a kind of anti-skidding chromatographic column.
Background technology
At present, the separation and purification of the composition of multiple compounds, natural products and Chinese medicine, is mainly to adopt column chromatography, uses chromatographic column, by column chromatography process, realizes.Traditional column chromatography is mixing cpd to be placed on to the top of glass column post bed, make the eluant, eluent (mobile phase) of mixed solvent composition under Action of Gravity Field, by the vertical glass column of absorbent media material (fixedly phase) is housed, separation obtains each purer product (sterling), its principle is: because each component in mixing cpd is to fixing different with the affinity of mobile phase mutually in chromatographic column, therefore select suitable fixedly phase and mobile phase according to the character of mixing cpd, can reach the separated as far as possible object of each component wherein, separated product is collected into respectively in each receiving flask in pillar outlet, then, identify the chromatographic solution of collecting in each receiving flask, according to qualification result, merge and concentrated each identical chromatographic solution, obtain each sterling.
Common chromatographic column can be divided into normal pressure post, middle compression leg and high-pressure column according to pressure difference.Normal pressure post is with the most use in practice, is usually the chromatographic column that oneself adopts as required different materials, loads the separation and purification that different fillers carrys out realize target material.Glass chromatography column is especially conventional again, often comprises cylinder, piston.At present there is following problem in common glass chromatography column: the chromatographic column outer surface smoother of glass material, holding chromatographic column or, all easily dropping fixedly in chromatographic column with clip, break chromatographic column, and its inner precious filler is trickled down.
In addition, current chromatographic column is when sample introduction, and sample solution directly flows to cylinder from the middle part on chromatographic column top, easily causes filler in cylinder to splash on the inwall of chromatographic column.The filler of using due to chromatography is usually more expensive, and the filler splashing on inwall is not fully utilized in chromatography, affects chromatography effect.
Summary of the invention
For addressing the above problem, the invention provides a kind of anti-skidding chromatographic column, adopt following technical scheme:
An anti-skidding chromatographic column, comprises column cap and cylinder, and described column cap, is characterized in that for being threaded with cylinder, and the top of described column cap is provided with the through hole passing through for silica gel hose; Described cylinder adopts transparent glass material; The top of described cylinder arranges dismountable U-shaped screen cloth, and described U-shaped screen cloth is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth; The outer surface of described cylinder arranges anti-skidding plastic jacket; The bottom of described cylinder is liquid outlet; Described liquid outlet comprises two mouthfuls; The top of described liquid outlet arranges sieve plate, and the aperture of described sieve plate is 0.2 ~ 0.4 μ m.
Preferably, the junction of described column cap and cylinder is provided with sealing ring, and described sealing ring is silica gel material.
The present invention has following beneficial effect:
1. chromatographic column provided by the invention, outer surface at cylinder arranges anti-skidding plastic jacket, so arranges, and is convenient to hold cylinder or cylinder is gripped on the top of the shelf, be difficult for chromatographic column to break, and avoid clip to leave cut on the cylinder of glass material; In addition, the observation for fear of plastic jacket impact to cylinder content, can select transparent plastic jacket.
2. chromatographic column provided by the invention, dismountable U-shaped screen cloth is set at the top of cylinder, described U-shaped screen cloth is the horn shape for upper end open, lower end closed, and the sidewall of U-shaped screen cloth contains through hole, so setting, when being conducive to buffer solution, sample liquid, eluent and entering from cylinder top, be that wall along cylinder flows down, can not cause splashing of liquid in cylinder, cause that filler splashes on the wall of cylinder, affect packed height, thereby affect the purification effect of albumen.
3. chromatographic column provided by the invention, top at liquid outlet arranges sieve plate, and the aperture that limits sieve plate is 0.2 ~ 0.4 μ m, filler in the time of so so just can reaching protein purification not can along with buffer solution, sample liquid, eluent together, from conical outlet, run off, but can not affect buffer solution, sample liquid and eluent from the outflow effect of conical outlet.
4. chromatographic column provided by the invention, middling speed qualitative filter paper filter membrane is placed on the top of U-shaped screen cloth, can carry out initial gross separation to sample liquid to be separated, and the larger impurity such as untreated thalline completely of interception size ratio, can also avoid the obstruction of U-shaped screen cloth.
5. chromatographic column provided by the invention, is provided with sealing ring in the junction of column cap and cylinder, is convenient to the airtight of column cap and cylinder, avoids buffer solution, sample liquid, eluent to flow out from both gaps, causes waste.
6. chromatographic column provided by the invention, liquid outlet comprises two mouthfuls, one mouthful is used for connecting product container, one mouthful is used for connecting waste fluid container, avoid changing the position of product container and waste fluid container when chromatography, thereby increase work efficiency, especially all larger at product container and waste fluid container, and while being difficult to move, the advantage of this kind of setting is more outstanding.
7. chromatographic column provided by the invention, simple in structure, easy to operate, and the purification effect of albumen is good.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention; Wherein, 1 represents column cap; 2 represent silica gel hose; 3 represent liquid outlet; 4 represent plastic jacket; 5 represent sieve plate; 6 represent U-shaped screen cloth; 7 represent cylinder.
The specific embodiment
Below in conjunction with drawings and Examples, the invention will be further described.Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of requirement of the present invention.
As shown in Figure 1, a kind of anti-skidding chromatographic column, comprises column cap 1 and cylinder 7, and described column cap 1, is characterized in that for being threaded with cylinder 7, and the top of described column cap 1 is provided with the through hole passing through for silica gel hose 2; Described cylinder 7 adopts transparent glass material; The top of described cylinder 7 arranges dismountable U-shaped screen cloth 6, and described U-shaped screen cloth 6 is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth 6 contains through hole; Middling speed qualitative filter paper filter membrane (do not mark in the drawings, it is arranged on the top of screen cloth, but is no more than the horizontal level at cylinder top) is placed on the top of described U-shaped screen cloth 6, and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth 6; The outer surface of described cylinder 7 arranges anti-skidding plastic jacket 4; The bottom of described cylinder 7 is liquid outlet 3; Described liquid outlet 3 comprises two mouthfuls; The top of described liquid outlet 3 arranges sieve plate 5, and the aperture of described sieve plate 5 is 0.2 ~ 0.4 μ m.
As preferably, in the junction of column cap 1 and cylinder 7, be provided with sealing ring (not marking out in the drawings), for sealing function, described sealing ring is silica gel material, sealing effectiveness is better.
Claims (2)
1. an anti-skidding chromatographic column, comprises column cap (1) and cylinder (7), and described column cap (1), is characterized in that for being threaded with cylinder (7), and the top of described column cap (1) is provided with the through hole passing through for silica gel hose (2); Described cylinder (7) adopts transparent glass material; The top of described cylinder (7) arranges dismountable U-shaped screen cloth (6), and described U-shaped screen cloth (6) is the horn shape of upper end open, lower end closed, and the sidewall of described U-shaped screen cloth (6) contains through hole; Middling speed qualitative filter paper filter membrane is placed on the top of described U-shaped screen cloth (6), and the surface area of described filter membrane is greater than or equal to the surface area of U-shaped screen cloth (6); The outer surface of described cylinder (7) arranges anti-skidding plastic jacket (4); The bottom of described cylinder (7) is liquid outlet (3); Described liquid outlet (3) comprises two mouthfuls; The top of described liquid outlet (3) arranges sieve plate (5), and the aperture of described sieve plate (5) is 0.2 ~ 0.4 μ m.
2. anti-skidding chromatographic column according to claim 1, is characterized in that, the junction of described column cap (1) and cylinder (7) is provided with sealing ring, and described sealing ring is silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644722.6A CN103691156B (en) | 2013-12-05 | 2013-12-05 | Antiskid chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644722.6A CN103691156B (en) | 2013-12-05 | 2013-12-05 | Antiskid chromatographic column |
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| Publication Number | Publication Date |
|---|---|
| CN103691156A true CN103691156A (en) | 2014-04-02 |
| CN103691156B CN103691156B (en) | 2016-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310644722.6A Active CN103691156B (en) | 2013-12-05 | 2013-12-05 | Antiskid chromatographic column |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106669230A (en) * | 2016-11-25 | 2017-05-17 | 南京海獭软件有限责任公司 | High-efficiency pressurized chromatographic column used in laboratory |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2090988U (en) * | 1991-01-09 | 1991-12-18 | 中国科学院大连化学物理研究所 | High-efficient liquid-phase chromatographic column |
| CN2511974Y (en) * | 2001-12-20 | 2002-09-18 | 刘开禄 | Large high efficiency preparation of chromatographic column |
| US7410571B2 (en) * | 2004-05-10 | 2008-08-12 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN202237384U (en) * | 2011-09-19 | 2012-05-30 | 何慧 | Chromatographic column for laboratory |
| CN202762139U (en) * | 2012-04-24 | 2013-03-06 | 齐齐哈尔大学 | Chromatographic column flowing phase device |
| CN203678011U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Antiskid chromatographic column |
-
2013
- 2013-12-05 CN CN201310644722.6A patent/CN103691156B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2090988U (en) * | 1991-01-09 | 1991-12-18 | 中国科学院大连化学物理研究所 | High-efficient liquid-phase chromatographic column |
| CN2511974Y (en) * | 2001-12-20 | 2002-09-18 | 刘开禄 | Large high efficiency preparation of chromatographic column |
| US7410571B2 (en) * | 2004-05-10 | 2008-08-12 | Scientific Plastic Products, Inc. | Flash chromatography cartridge |
| CN201379989Y (en) * | 2009-01-05 | 2010-01-13 | 栗云天 | Chromatographic column assembly |
| CN202237384U (en) * | 2011-09-19 | 2012-05-30 | 何慧 | Chromatographic column for laboratory |
| CN202762139U (en) * | 2012-04-24 | 2013-03-06 | 齐齐哈尔大学 | Chromatographic column flowing phase device |
| CN203678011U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Antiskid chromatographic column |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106669230A (en) * | 2016-11-25 | 2017-05-17 | 南京海獭软件有限责任公司 | High-efficiency pressurized chromatographic column used in laboratory |
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| CN103691156B (en) | 2016-08-31 |
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