CN103687873B - 鉴定亲和力成熟的人类抗体 - Google Patents

鉴定亲和力成熟的人类抗体 Download PDF

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Publication number
CN103687873B
CN103687873B CN201280034673.8A CN201280034673A CN103687873B CN 103687873 B CN103687873 B CN 103687873B CN 201280034673 A CN201280034673 A CN 201280034673A CN 103687873 B CN103687873 B CN 103687873B
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primers
region
leader
pcr
human
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Chinese (zh)
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CN103687873A (zh
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J·西马德
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XBIOTECH Inc
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XBIOTECH Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201280034673.8A 2011-07-12 2012-07-12 鉴定亲和力成熟的人类抗体 Active CN103687873B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610954453.7A CN107090459A (zh) 2011-07-12 2012-07-12 鉴定亲和力成熟的人类抗体

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161506749P 2011-07-12 2011-07-12
US61/506,749 2011-07-12
PCT/US2012/046431 WO2013009967A2 (en) 2011-07-12 2012-07-12 Identifying affinity-matured human antibodies

Related Child Applications (1)

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CN201610954453.7A Division CN107090459A (zh) 2011-07-12 2012-07-12 鉴定亲和力成熟的人类抗体

Publications (2)

Publication Number Publication Date
CN103687873A CN103687873A (zh) 2014-03-26
CN103687873B true CN103687873B (zh) 2016-10-19

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CN201280034673.8A Active CN103687873B (zh) 2011-07-12 2012-07-12 鉴定亲和力成熟的人类抗体
CN201610954453.7A Pending CN107090459A (zh) 2011-07-12 2012-07-12 鉴定亲和力成熟的人类抗体

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CN201610954453.7A Pending CN107090459A (zh) 2011-07-12 2012-07-12 鉴定亲和力成熟的人类抗体

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US (6) US9234030B2 (enExample)
EP (2) EP2731965A4 (enExample)
JP (1) JP6731702B2 (enExample)
KR (1) KR102139388B1 (enExample)
CN (2) CN103687873B (enExample)
AU (2) AU2012281078B2 (enExample)
CA (1) CA2841475C (enExample)
IL (2) IL230358B (enExample)
MX (2) MX361600B (enExample)
RU (2) RU2636045C2 (enExample)
WO (1) WO2013009967A2 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102139388B1 (ko) * 2011-07-12 2020-07-30 엑스바이오테크, 인크. 친화성 성숙 인간 항체의 동정
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
GB201316644D0 (en) * 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
JP6647197B2 (ja) * 2014-05-30 2020-02-14 国立大学法人富山大学 ＴＣＲ ｃＤＮＡの増幅方法
WO2015187779A1 (en) 2014-06-03 2015-12-10 Xbiotech, Inc. Compositions and methods for treating and preventing staphylococcus aureus infections
SG10201911069WA (en) 2014-09-15 2020-01-30 Abvitro Llc High-throughput nucleotide library sequencing
US10975146B2 (en) 2018-06-29 2021-04-13 Cedars-Sinai Medical Center Interleukin-1 inhibition for combination treatment of pancreatic cancer
US20200392578A1 (en) * 2019-06-14 2020-12-17 The Regents Of The University Of California Methods of sequencing antibody chains from hybridomas and kits for practicing same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010094475A1 (en) * 2009-02-20 2010-08-26 F. Hoffmann-La Roche Ag Method for obtaining immunoglobulin encoding nucleic acid

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001212019B2 (en) 2000-03-10 2006-12-14 Large Scale Biology Corporation Self antigen vaccines for treating b cell lymphomas and other cancers
AU2002953381A0 (en) * 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
CN1860367B (zh) * 2003-07-29 2010-05-26 健泰科生物技术公司 人抗cd20抗体的测定法及其用途
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
PE20071101A1 (es) 2005-08-31 2007-12-21 Amgen Inc Polipeptidos y anticuerpos
JP5205597B2 (ja) * 2007-06-01 2013-06-05 静岡県 １細胞レベルでの抗体遺伝子の解析・同定方法
US8404445B2 (en) * 2008-09-30 2013-03-26 Abbvie Inc. Antibody libraries
KR102139388B1 (ko) * 2011-07-12 2020-07-30 엑스바이오테크, 인크. 친화성 성숙 인간 항체의 동정

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010094475A1 (en) * 2009-02-20 2010-08-26 F. Hoffmann-La Roche Ag Method for obtaining immunoglobulin encoding nucleic acid

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
A new subgroup of immunoglobulin heavy chain variable region genes for the assessment of clonality in feline B-cell lymphomas;M. Henrich，et al.;《Veterinary Immunology and Immunopathology》;20090715;第130卷(第1-2期);第59–69页 *
High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation;Dirk Ponsel，et al.;《Molecules》;20110503;第16卷(第5期);第3675-3700页 *
Improving antibody affinity by mimicking somatic hypermutation in vitro;Partha S. Chowdhury，et al.;《Nature biotechnology》;19990630;第17卷(第6期);第568-572页 *
Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire;Jacob Glanville，et al.;《PNAS》;20091201;第106卷(第48期);摘要，第20217页右栏第7段和第20220页右栏最后1段 *

Also Published As

Publication number Publication date
EP3034513A2 (en) 2016-06-22
AU2012281078A1 (en) 2014-01-23
AU2012281078B2 (en) 2017-05-04
US9394536B2 (en) 2016-07-19
WO2013009967A3 (en) 2013-04-04
WO2013009967A2 (en) 2013-01-17
RU2762685C2 (ru) 2021-12-22
US20160060618A1 (en) 2016-03-03
CA2841475C (en) 2021-02-09
EP3034513B1 (en) 2020-09-02
IL230358B (en) 2018-11-29
RU2014102617A (ru) 2015-08-20
RU2017134418A (ru) 2019-02-07
JP2014521316A (ja) 2014-08-28
JP6731702B2 (ja) 2020-07-29
US20130018173A1 (en) 2013-01-17
CN107090459A (zh) 2017-08-25
EP3034513A3 (en) 2016-08-10
RU2636045C2 (ru) 2017-11-17
KR20140044905A (ko) 2014-04-15
MX361600B (es) 2018-12-11
US9670483B2 (en) 2017-06-06
MX2014000431A (es) 2014-09-04
KR102139388B1 (ko) 2020-07-30
CN103687873A (zh) 2014-03-26
US20170233457A1 (en) 2017-08-17
MX2018015450A (es) 2021-11-16
IL262977B (en) 2020-07-30
EP2731965A4 (en) 2015-06-03
RU2017134418A3 (enExample) 2021-06-10
US9453217B2 (en) 2016-09-27
IL262977A (en) 2018-12-31
US20200071388A1 (en) 2020-03-05
CA2841475A1 (en) 2013-01-17
AU2017210585B2 (en) 2019-07-25
US20160362679A1 (en) 2016-12-15
AU2017210585A1 (en) 2017-08-24
EP2731965A2 (en) 2014-05-21
US9234030B2 (en) 2016-01-12
US20160060619A1 (en) 2016-03-03

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