CN103675307A - Non-contact reaction cup flushing device and flushing method thereof - Google Patents
Non-contact reaction cup flushing device and flushing method thereof Download PDFInfo
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Abstract
The invention relates to a non-contact reaction cup flushing device and a flushing method thereof and belongs to the technical field of bio-medical instruments. The non-contact reaction cup flushing device comprises a pump unit, a liquid injection pipe, a liquid suction pipe, a position control unit and a control circuit. The invention also discloses the flushing method of the non-contact reaction cup flushing device. The non-contact reaction cup flushing device is non-complicated in structure; the control circuit is matched with a control program and a mechanical structure to control the liquid injection pipe and the liquid suction pipe not to contact with liquid in a reaction cup, so that the outer walls of the liquid injection pipe and the liquid suction pipe do not pollute the liquid in the reaction cup and cannot carry reactants in the reaction cup; therefore, the cross contamination between different reaction cups is avoided, and errors of an inspection result of a medical inspection instrument are reduced.
Description
Technical field
The present invention relates to a kind of reaction cup flusher and purging method thereof for medical laboratory instrument, belong to Biomedical Instruments technical field.
Background technology
Conventional medical laboratory instrument, as Full-automatic chemiluminescence immunoassay analysis meter, full automatic biochemical apparatus etc., for analyzing some composition of human sample or other biological sample, can check disease (as check human diseases), identification of organism (as the kind of Identifying micro-organisms) etc.Medical laboratory instrument generally all can relate to flushing link in checkout procedure, for example: Full-automatic chemiluminescence immunoassay analysis meter in course of reaction by the means of rinsing by reaction cup except the sample material by the antigen of antibody capture rinses out.Full automatic biochemical apparatus can relate to flushing link equally in course of reaction.Existing reaction cup douching technique, is generally by flushing needle being inserted in reaction cup, liquid being taken away, then injects washing fluid in reaction cup, and then liquid is taken away, so repeatedly.Existing reaction cup douching technique is generally contact, that is: flushing needle will contact with the liquid in reaction cup, has cross-contamination issue.The accuracy of cross pollution meeting interference test result.For example application number is 200720067580.1, name is called the Chinese patent application of " wiper mechanism of automatic clinical chemistry analyzer ", and for example application number is 201020115433.9, name is called the Chinese patent application of " a kind of reaction cup wash mill for automatic lmunoassays analyzer ", these prior aries are all contact douching techniques, all have cross-contamination issue.
Therefore, need a kind of new contactless reaction cup flusher to address the above problem.
Summary of the invention
The present invention is directed to the defect of contact reaction cup flusher in prior art, a kind of contactless reaction cup flusher system of stopping cross pollution is provided, and provides this system corresponding purging method.
For solving the problems of the technologies described above, the technical scheme that contactless reaction cup flusher of the present invention adopts is:
A contactless reaction cup flusher, is characterized in that, comprises pump unit, liquid injection pipe, pipette, position control unit, control circuit; Liquid injection pipe, pipette are connected with pump unit, and position control unit regulates the locus of liquid injection pipe, pipette; Control circuit is provided with microprocessor, microprocessor executive control program, in control circuit, be provided with sensing circuit, the duty of control circuit control pump unit and position control unit, control circuit is monitored pipette, the liquid injection pipe position in reaction cup by sensing circuit, and/or control circuit is monitored the distance between the liquid level of liquid in pipette, liquid injection pipe and reaction cup by sensing circuit.
Further, described position control unit comprise motor, slide rail, screw mandrel, support, for the fixing movable block of liquid injection pipe and pipette; Motor drives movable block to move on slide rail by screw mandrel, and support is played a supporting role.
Further, described pump unit comprises fluid injection and two kinds of functional modules of imbibition, and described liquid injection pipe is connected with the fluid injection module of pump unit, and described pipette is connected with the imbibition module of pump unit.
Further, described control circuit is monitored the parameter of pipette inner fluid by sensing circuit.
Further, described control circuit is monitored the parameter of liquid injection pipe inner fluid by sensing circuit.
Further, described control circuit is monitored the liquid parameter in reaction cup by sensing circuit.
Further, described liquid injection pipe and/or pipette and described control circuit are electrically connected, and control circuit is usingd liquid injection pipe and/or pipette as the liquid level of liquid in probe detection reaction cup and the relative position between liquid injection pipe and/or pipette.
Beneficial effect: contactless reaction cup flusher of the present invention, structure is uncomplicated, by coordinating of control circuit and control program and physical construction, realizing control liquid injection pipe does not contact with liquid in pipette and reaction cup, the outer wall of having guaranteed liquid injection pipe and pipette does not pollute liquid in reaction cup, does not carry the reactant in reaction cup yet, stop the cross pollution between differential responses cup, reduced the error of medical laboratory instrument assay.
The invention also discloses the purging method of above-mentioned contactless reaction cup flusher, it is characterized in that: in flushing process, described liquid injection pipe does not contact with the liquid in reaction cup with pipette; When imbibition, rely on pump unit to produce negative pressure by near the air pipette mouth of pipe, and then drive the liquid in reaction cup to enter pipette; When fluid injection, rely on inertial flow and the Action of Gravity Field of liquid, do not need liquid injection pipe to contact with the liquid in reaction cup.
Further, the purging method of the contactless reaction cup flusher of the above, described liquid injection pipe does not contact with the liquid in reaction cup with pipette, and the distance of controlling by control circuit between the liquid level of liquid in liquid injection pipe and pipette and reaction cup realizes; Distance in detection liquid injection pipe and pipette and reaction cup between the liquid level of liquid, its implementation can adopt any in following methods, or also uses wherein at least two kinds:
Method one, described liquid injection pipe and pipette and described control circuit are electrically connected, liquid injection pipe and pipette are as probe, relative position between liquid in reaction cup and liquid injection pipe, pipette can affect the capacitance parameter between liquid injection pipe and pipette, capacitance variations between control circuit monitoring liquid injection pipe and pipette, thereby the liquid level of the interior liquid of detection reaction cup and the relative position between liquid injection pipe and pipette;
Method two, described liquid injection pipe or pipette and described control circuit are electrically connected, liquid injection pipe or pipette are as probe, in the position that approaches reaction cup, reference electrode is set, liquid in reaction cup is between liquid injection pipe or pipette and reference electrode, liquid measure in reaction cup affects the capacitance parameter between liquid injection pipe or pipette and reference electrode, capacitance variations in control circuit monitoring liquid injection pipe or pipette, thereby the liquid level of the interior liquid of detection reaction cup and the relative position between liquid injection pipe or pipette;
Method three, utilizes liquid level to light wave or electromagnetic reflection, light wave or electromagnetic wave sensing device is set on position control unit whether to have liquid in detection reaction cup and to survey liquid level position;
Method four, utilizes liquid level to sound wave or hyperacoustic reflection, sound wave or supersonic sensing device is set on position control unit whether to have liquid in detection reaction cup and to survey liquid level position;
Method five, utilizes liquid level to light wave or electromagnetic reflection, light wave or electromagnetic wave sensing device is set in liquid injection pipe and/or pipette whether to have liquid in detection reaction cup and to survey liquid level position;
Method six, utilizes liquid level to sound wave or hyperacoustic reflection, sound wave or supersonic sensing device is set in liquid injection pipe and/or pipette whether to have liquid in detection reaction cup and to survey liquid level position;
Method seven, on the connecting line of described pipette, pressure sensor device is set, described control circuit obtains the pressure value in pipette by pressure sensor device, and control circuit is changed and come the liquid level of liquid in detection reaction cup and the relative position between pipette by the pressure value in analysis pipette; In the situation that the attraction power of pipette is constant, when the mouth of pipe of pipette approaches the liquid level of liquid in reaction cup, the pressure in pipette can change.
Above-mentioned detection method is all known technology.
Further, the purging method of the contactless reaction cup flusher of the above, comprises the following steps:
Steps A, described position control unit drive described liquid injection pipe and pipette to enter in reaction cup, when the distance in the mouth of pipe of pipette and reaction cup between the liquid level of liquid reaches default threshold value, when the speed control liquid injection pipe obtaining according to control program calculating at control circuit and pipette move down, pipette according to control program calculate obtain power carry out imbibition, liquid in reaction cup is sucked, stop moving down;
Step B, described position control unit drive described liquid injection pipe and pipette up to move default distance according to default speed, then when the speed obtaining according to control program calculating is up mobile, by control program, calculate the flow velocity obtaining and inject cleaning fluid in reaction cup, until inject default liquid measure, stop up moving and fluid injection;
Step C, described position control unit drive described liquid injection pipe and pipette to calculate according to control program the speed obtaining and move down, in mobile, pump unit calculates the power imbibition obtaining according to control program, liquid in reaction cup is sucked, stop moving down; So far, complete once flushing;
Step D, described control circuit carry out above-mentioned step B to the multiplicity of step C according to default washing time; After completing required washing time, described position control unit resets, and described liquid injection pipe and pipette are got back to initial position.
Above-mentioned rinsing step is all to carry out under the control of control circuit.
Beneficial effect: the purging method of contactless reaction cup flusher of the present invention, when imbibition, the distance of controlling by control circuit between the liquid level of liquid in the mouth of pipe reaction cup of pipette maintains in default threshold range, utilize the suction function of air to drive liquid to enter in pipette, simultaneously also because the effect of negative pressure, the drop that liquid in pipette or pipette outer wall may speckle with can not flow backwards and enter reaction cup, has realized contactless imbibition; When fluid injection, rely on inertial flow and the Action of Gravity Field of liquid, liquid injection pipe is without contacting with the liquid in reaction cup, and, liquid injection pipe is in calculating the process of moving in the speed obtaining according to program, according to program, to calculate the flow velocity obtaining to carry out fluid injection, rather than etc. move on to larger distance and position on liquid injection pipe and just start fluid injection, the drop sputter of having avoided liquid to produce from injecting compared with large distance; The present invention has realized liquid injection pipe in flushing process and has not contacted with liquid in reaction cup with pipette, guaranteed that liquid injection pipe and pipette do not pollute liquid in reaction cup, do not carry the reactant in reaction cup yet, stop the cross pollution between differential responses cup, improved the reliability of medical laboratory instrument assay.
Accompanying drawing explanation
Fig. 1 is schematic diagram, for a specific embodiment of contactless reaction cup flusher of the present invention is described;
Fig. 2 is schematic diagram, for the electric connecting relation of the specific embodiment shown in key diagram 1;
Fig. 3 is schematic diagram, for the principle of the contactless imbibition of institute of the present invention foundation is described;
Wherein, 1-liquid injection pipe, 2-inhales pipe at night, 3-pump unit, 4-fluid sensing device one, 5-movable block, 6-motor, 7-support, 8-screw mandrel, 9-slide rail, 10-position sensing switch one, 11-position sensing switch two, 12-reaction cup, the liquid in 13-reaction cup, 14-fluid sensing device two, 15-control circuit, near the negative pressuren zone a1-pipette mouth of pipe.
Embodiment
Below in conjunction with the drawings and specific embodiments, further illustrate the present invention, should understand these embodiment is only not used in and limits the scope of the invention for the present invention is described, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the modification of the various equivalent form of values of the present invention.
A specific embodiment of the present invention as shown in Figure 1 to Figure 3.Comprise pump unit 3, liquid injection pipe 1, pipette 2, position control unit, control circuit 15; Liquid injection pipe 1, pipette 2 are connected with pump unit 3; Pump unit 3 comprises fluid injection and two kinds of functional modules of imbibition, and liquid injection pipe 1 is connected with the fluid injection module of pump unit 3, and pipette 2 is connected with the imbibition module of pump unit 3; Position control unit regulates the locus of liquid injection pipe 1, pipette 2; In control circuit 15, be provided with microprocessor, microprocessor executive control program, is provided with sensing circuit in control circuit 15, the duty (as shown in Figure 2) of control circuit 15 control pump unit 3 and position control unit.Position control unit comprises motor 6, slide rail 9, screw mandrel 8, support 7, for the fixing movable block 5 of liquid injection pipe 1 and pipette 2; Motor 6 adopts stepper motor, and motor 6 drives movable block 5 to move on slide rail 9 by screw mandrel 8, and support 7 is played a supporting role.Liquid injection pipe 1 and pipette 2 are fixed on the movable block 5 of position control unit.Two position sensing devices are set on the support 7 of position control unit, i.e. position sensing switch 1 and position sensing switch 2 11; Movable block 5 up mobile trigger position sensitive switch means that position control unit is reset to initial position at 2 11 o'clock; The movable block 5 trigger position sensitive switch that moves down means that movable block 5 has arrived the limit moving down at one 10 o'clock, and namely liquid injection pipe 1 and pipette 2 have arrived lower limit, and now the mouth of pipe of liquid injection pipe 1 and pipette 2 approaches reaction cup 12 inner bottom parts; Position sensing switch 1 and position sensing switch 2 11 are ingredients of the sensing circuit of control circuit 15.
In this specific embodiment of the present invention, on the connecting line between pipette 2 and pump unit 3, be connected a fluid sensing device 1.Fluid sensing device 1 is ingredients of the sensing circuit of control circuit 15.Fluid sensing device 1 is a kind of optical device, the transmitting terminal emission detection light of fluid sensing device 1, receiving end receives surveys light, flowing through the transmitting terminal of fluid sensing device 1 and the liquid in the space between receiving end can be to surveying light exert an influence (being mainly that light intensity changes and Doppler effect occurs), if flowed through, the fluid of fluid sensing device 1 is potpourris of air and liquid, also can exert an influence to surveying light.Control circuit 15 is by the parameter of fluid sensing device one 4 monitoring pipette 2 inner fluids, to judge whether pipette 2 is drawn onto the flow velocity of liquid and liquid, the numerical value that comprehensively position of pipette 2, imbibition duration and fluid sensing device 1 return judges whether the liquid in reaction cup 12 to be sucked.Control circuit 15 is stepper motor by motor 6() sense of rotation and with respect to the accumulative total step number of position sensing switch 1 or position sensing switch 2 11, the position of calculating pipette 2 and liquid injection pipe 1.
As a same reason, on the connecting line between liquid injection pipe 1 and pump unit 3, be connected fluid sensing device 2 14, realize the parameter of monitoring liquid injection pipe 1 inner fluid, for example, monitor the interior flow rate of liquid of liquid injection pipe 1, can calculate the liquid measure of injecting reaction cup.Also can be by a micro-ultrasonic wave sensor be set between liquid injection pipe 1 and pipette 2, the reflection producing while calculating ultrasound wave through liquid level and through reaction cup bottom liquid the time difference with the reflection producing during interface at the bottom of reaction cup cup, calculate the degree of depth of liquid in reaction cup.
As shown in Figure 2, liquid injection pipe 1 and pipette 2 are electrically connected with control circuit 15, control circuit 15 is usingd liquid injection pipe 1 and pipette 2 as probe, by analyzing the mode of the electric capacity between liquid injection pipe 1 and pipette 2, comes the liquid level of liquid in detection reaction cup and the relative position between liquid injection pipe 1 and pipette 2.Relative position between liquid in reaction cup and liquid injection pipe 1, pipette 2 can affect the capacitance parameter between liquid injection pipe 1 and pipette 2; The relative position of the liquid in reaction cup and liquid injection pipe 1, pipette 2 is more approaching, and the capacitance between liquid injection pipe 1 and pipette 2 is just larger.In flushing process, the capacitance variations between control circuit 15 monitoring liquid injection pipes 1 and pipette 2.
As shown in Figure 3, the present invention realizes the principle of contactless imbibition institute foundation, and under the attraction of pump unit 3, near air pipette 2 mouths of pipe is negative pressure, produces near the negative pressuren zone a1 pipette mouth of pipe; In the situation that the attraction power of pump unit 3 is constant, distance in the mouth of pipe of pipette 2 and reaction cup between the liquid level of liquid is little in certain threshold range time, and when negative pressure reaches certain numerical value, liquid 13 in reaction cup is by the air extrusion in the larger region of pressure, toward the low position of air pressure, move, thereby enter pipette 2, the air-flow that air pressure difference produces also can promote liquid toward the interior movement of pipette 2.Realize liquid injection pipe 1 in flushing process and do not contact with the liquid in reaction cup with pipette 2, need to realize by the distance between the liquid level of liquid in control circuit 15 control liquid injection pipes 1 and pipette 2 and reaction cup.
Purging method with regard to this specific embodiment of the present invention below describes:
Step 4, control circuit 15 carries out above-mentioned step 2 to the multiplicity of step 3 according to default washing time; After completing required washing time, position control unit resets, and liquid injection pipe 1 and pipette 2 are got back to initial position.
Above-mentioned rinsing step is all to carry out under the control of control circuit 15.
Claims (10)
1. a contactless reaction cup flusher, is characterized in that: comprise pump unit (3), liquid injection pipe (1), pipette (2), position control unit, control circuit (15), liquid injection pipe (1), pipette (2) is connected with pump unit (3), position control unit regulates liquid injection pipe (1), the locus of pipette (2), control circuit is provided with microprocessor in (15), microprocessor executive control program, control circuit (15) is provided with sensing circuit, the duty of control circuit (15) control pump unit (3) and position control unit, control circuit (15) is monitored pipette (2) by sensing circuit, the position of liquid injection pipe (1) in reaction cup, and/or control circuit (15) is monitored pipette (2) by sensing circuit, distance in liquid injection pipe (1) and reaction cup between the liquid level of liquid.
2. contactless reaction cup flusher according to claim 1, is characterized in that: described position control unit comprises motor (6), slide rail (9), screw mandrel (8), support (7), for the fixing movable block (5) of liquid injection pipe (1) and pipette (2); Motor (6) drives movable block (5) upper mobile at slide rail (9) by screw mandrel (8), and support (7) is played a supporting role.
3. contactless reaction cup flusher according to claim 1, it is characterized in that: described pump unit (3) comprises fluid injection and two kinds of functional modules of imbibition, described liquid injection pipe (1) is connected with the fluid injection module of pump unit (3), and described pipette (2) is connected with the imbibition module of pump unit (3).
4. contactless reaction cup flusher according to claim 1, is characterized in that: described control circuit (15) is monitored the parameter of pipette (2) inner fluid by sensing circuit.
5. contactless reaction cup flusher according to claim 1, is characterized in that: described control circuit (15) is monitored the parameter of liquid injection pipe (1) inner fluid by sensing circuit.
6. contactless reaction cup flusher according to claim 1, is characterized in that: described control circuit (15) is monitored the liquid parameter in reaction cup by sensing circuit.
7. contactless reaction cup flusher according to claim 1, it is characterized in that: described liquid injection pipe (1) and/or pipette (2) are electrically connected with described control circuit (15), control circuit (15) is usingd liquid injection pipe (1) and/or pipette (2) as the liquid level of liquid in probe detection reaction cup and the relative position between liquid injection pipe (1) and/or pipette (2).
8. according to the purging method of the contactless reaction cup flusher described in claim 1-7 any one, it is characterized in that: in flushing process, described liquid injection pipe (1) does not contact with the liquid in reaction cup with pipette (2).
9. the purging method of contactless reaction cup flusher according to claim 8, it is characterized in that: described liquid injection pipe (1) does not contact with the liquid in reaction cup with pipette (2), the distance of controlling between liquid injection pipe (1) and pipette (2) and the liquid level of the interior liquid of reaction cup by control circuit (15) realizes; Distance in detection liquid injection pipe (1) and pipette (2) and reaction cup between the liquid level of liquid, its implementation can adopt any in following methods, or also uses wherein at least two kinds:
Method one, described liquid injection pipe (1) and pipette (2) are electrically connected with described control circuit (15), liquid injection pipe (1) and pipette (2) are as probe, relative position between liquid in reaction cup and liquid injection pipe (1), pipette (2) can affect the capacitance parameter between liquid injection pipe (1) and pipette (2), capacitance variations between control circuit (15) monitoring liquid injection pipe (1) and pipette (2), thereby the liquid level of the interior liquid of detection reaction cup and the relative position between liquid injection pipe (1) and pipette (2);
Method two, described liquid injection pipe (1) or pipette (2) are electrically connected with described control circuit (15), liquid injection pipe (1) or pipette (2) are as probe, in the position that approaches reaction cup, reference electrode is set, liquid in reaction cup is between liquid injection pipe (1) or pipette (2) and reference electrode, liquid measure in reaction cup affects the capacitance parameter between liquid injection pipe (1) or pipette (2) and reference electrode, capacitance variations in control circuit (15) monitoring liquid injection pipe (1) or pipette (2), thereby the liquid level of liquid and the relative position between liquid injection pipe (1) or pipette (2) in detection reaction cup,
Method three, utilizes liquid level to light wave or electromagnetic reflection, light wave or electromagnetic wave sensing device is set on position control unit whether to have liquid in detection reaction cup and to survey liquid level position;
Method four, utilizes liquid level to sound wave or hyperacoustic reflection, sound wave or supersonic sensing device is set on position control unit whether to have liquid in detection reaction cup and to survey liquid level position;
Method five, utilizes liquid level to light wave or electromagnetic reflection, light wave or electromagnetic wave sensing device is set in liquid injection pipe (1) and/or pipette (2) and whether has liquid in detection reaction cup and survey liquid level position;
Method six, utilizes liquid level to sound wave or hyperacoustic reflection, sound wave or supersonic sensing device is set in liquid injection pipe (1) and/or pipette (2) and whether has liquid in detection reaction cup and survey liquid level position;
Method seven, on the connecting line of described pipette (2), pressure sensor device is set, described control circuit (15) obtains the pressure value in pipette (2) by pressure sensor device, and control circuit (15) is changed and come the liquid level of liquid in detection reaction cup and the relative position between pipette (2) by the pressure value in analysis pipette (2); In the situation that the attraction power of pipette (2) is constant, when the mouth of pipe of pipette (2) approaches the liquid level of liquid in reaction cup, the pressure in pipette (2) can change.
10. the purging method of contactless reaction cup flusher according to claim 8, is characterized in that, comprises the following steps:
Steps A, described position control unit drive described liquid injection pipe (1) and pipette (2) to enter in reaction cup, when the distance in the mouth of pipe of pipette (2) and reaction cup between the liquid level of liquid reaches default threshold value, when the speed control liquid injection pipe (1) obtaining according to control program calculating at control circuit (15) and pipette (2) move down, pipette (2) according to control program calculate obtain power carry out imbibition, liquid in reaction cup is sucked, stop moving down;
Step B, described position control unit drive described liquid injection pipe (1) and pipette (2) up to move default distance according to default speed, then when the speed obtaining according to control program calculating is up mobile, by control program, calculate the flow velocity obtaining and inject cleaning fluid in reaction cup, until inject default liquid measure, stop up moving and fluid injection;
Step C, described position control unit drive described liquid injection pipe (1) and pipette (2) to calculate according to control program the speed obtaining and move down, pump unit (3) described in mobile calculates the power imbibition obtaining according to control program, liquid in reaction cup is sucked, stop moving down; So far, complete once flushing;
Step D, described control circuit (15) carry out above-mentioned step B to the multiplicity of step C according to default washing time; After completing required washing time, described position control unit resets, and described liquid injection pipe (1) and pipette (2) are got back to initial position.
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| CN201310581761.6A CN103675307A (en) | 2013-11-19 | 2013-11-19 | Non-contact reaction cup flushing device and flushing method thereof |
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| CN105834176A (en) * | 2016-05-20 | 2016-08-10 | 威海威高生物科技有限公司 | Convenient and fast cleaning device of full-automatic chemiluminescence tester |
| CN106037734A (en) * | 2016-06-29 | 2016-10-26 | 苏州海神联合医疗器械有限公司 | Myoelectricity surface electrode head with cleaning function and cleaning method adopted by same |
| CN109550533A (en) * | 2017-09-26 | 2019-04-02 | 深圳市新产业生物医学工程股份有限公司 | The condition detection method of liquid-transfering device and its liquid-transfering needle |
| CN110456094A (en) * | 2018-05-08 | 2019-11-15 | 赛多利斯百得液体处理公司 | Liquid processing system and method for analyzing suction nozzle state |
| CN110711751A (en) * | 2018-07-11 | 2020-01-21 | 深圳迈瑞生物医疗电子股份有限公司 | Automatic cleaning apparatus, control method of automatic cleaning apparatus, and storage medium |
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| CN109550533A (en) * | 2017-09-26 | 2019-04-02 | 深圳市新产业生物医学工程股份有限公司 | The condition detection method of liquid-transfering device and its liquid-transfering needle |
| CN109550533B (en) * | 2017-09-26 | 2021-10-08 | 深圳市新产业生物医学工程股份有限公司 | Pipetting device and method for detecting state of pipetting needle thereof |
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