CN103653147B - Wheat peptide anti-fatigue health drink and preparation method thereof - Google Patents

Wheat peptide anti-fatigue health drink and preparation method thereof Download PDF

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CN103653147B
CN103653147B CN201310706116.2A CN201310706116A CN103653147B CN 103653147 B CN103653147 B CN 103653147B CN 201310706116 A CN201310706116 A CN 201310706116A CN 103653147 B CN103653147 B CN 103653147B
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juice
vitamin
wheat
peptide
oligopeptide
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CN103653147A (en
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陈燕飞
刘良忠
朱学良
江思佳
刘闪
杨永生
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WUHAN PASHUN ZHENGYUAN BIOTECH AND ENGINEERING Co Ltd
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WUHAN PASHUN ZHENGYUAN BIOTECH AND ENGINEERING Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention provides a wheat peptide anti-fatigue health drink and a preparation method thereof. The preparation method comprises the following steps: firstly mixing 1-5% of medlar, 1-5% of hawthorn, 0.2-2% of honeysuckle and 0.5-5% of lophatherum gracile, adding water in the mixture to boil, filtering the solution, adding 0.5-5% of wheat oligopeptide, 0.5-5% of sesame protein peptide, 5-12% of sugar or honey, 0-0.06% of taurine, 0.025-0.05% of vitamins, 5-15% of fruit and vegetable juice or powder and 0.1-0.3% of citric acid in the filtered mixed liquor, using purified water to dilute the mixed liquor, mixing uniformly and filtering, sterilizing for 6-30 seconds at 85-135 DEG C, filling and closing the cover, and performing water bath to sterilize the mixed liquor for 15-45 seconds at 85-98 DEG C, and cooling to prepare the finished health drink. The prepared health drink has the anti-fatigue function, multiple health functions, is tasty in flavor, free from side effect, and is an ideal health drink.

Description

A kind of wheat peptide anti-fatigue health drink and preparation method thereof
Technical field
The invention provides a kind of functional beverage, specifically a kind of antifatigue wheat peptide drink and preparation method thereof.
Background technology
Along with socioeconomic development, the quickening of people's rhythm of life, work is selected complicated, variation is large, social competition's pressure also constantly increases, thus fatigue, spirit are indefinite, body consumptive disease damages and healthy imbalance to cause most of staff to occur, and physical fatigue is a kind of physiological phenomenon, all impact can be brought to the work of people and life, health long-term disposal fatigue state, hormone in vivo metabolism disorder, nervous system regulatory function exception, immunity reduction can be caused, the self-disciplining nervous disorder symptoms such as shoulder is ached, headache can be caused; More corresponding healths itself suffer from the people of the disease such as blood vessel, heart, if overworked at ordinary times to cause sudden death.
Solution for fatigue has the alleviation of drug therapy and food, the conditioning comparing drug therapy food is better to health, there are a lot of antifatigue Food & Drink in existing market, but its effect of food of a lot of antifatigue is not fine, can only of short duration elimination physical fatigue, but also side effect can be brought to health, particularly in a lot of anti-fatigue beverage also containing caffeine, took in and multipairly also can bring injury to health.
Wheat protein peptide is a kind of vegetalitas biologically active peptide deriving from wheat gluten, is take wheat gluten as raw material, and the micromolecule polypeptide material adopting protease hydrolytic to obtain, has good water solubility, stably dispersing, easily absorb, the features such as biologically active is strong; In the amino acid composition of wheat peptide, glutamic acid, proline, leucine content are high, and especially content of glutamic acid accounts for about 35% of total amino acid content, and medically glutamic acid is mainly used in treating hepatic coma, also for improving children ' s intelligence development.After glutamic acid is absorbed by the body, easily forms paddy acyl ammonia with blood ammonia, the toxic action of ammonia in metabolic process can be removed, thus can prevention and therapy hepatic coma, protecting liver, is the ancillary drug of patients with liver diseases.Brain tissue can only be oxidized glutamic acid, and can not be oxidized other amino acid, therefore glutamine can be used as the energy matter of brain tissue, improves and maintains cerebral function.Glutamic acid as nerve center and corticocerebral tonic, for treatment cerebral concussion or neurotrosis, epilepsy and all have certain curative effect to mentally retarded child.
Experimental study also shows, wheat Toplink significantly improves SOD vigor and TAC (T-AOC) in TAC in serum (T-AOC), GSH-Px, SOD vigor and heart tissue, also TAC (T-AOC) in GSH-Px vigor and brain to be significantly improved in brain and liver organization, significantly can reduce the MDA content in serum and each tissue simultaneously, and then play effect that is anti-oxidant in body and antifatigue.In addition, wheat peptide also has different physiological roles such as suppressing cholesterol rising, ACE suppression, immunological regulation.And at present do not have people to be applied to by wheat peptide to prepare in drink formula.
Sesame protein content is generally 14%-17%, can reach 29.7%, it tires close with animal protein, a kind of good protein, sesame protein amino acid content enriches, 18 seed amino acid content are reasonable, and its sesame peptide has good antifatigue, the effect such as hypotensive and anti-oxidant, but in the health food sesame peptide not being applied to preparation antifatigue at present and drink.
Summary of the invention
The object of this invention is to provide a kind of utilize wheat peptide and sesame peptide to be prepared from a kind of Anti-fatigue drink and preparation method; this drink is using wheat peptide and sesame peptide two kinds of natural plants peptides as the main component of antifatigue; and the natural component of the integration of drinking and medicinal herbs such as auxiliary honeysuckle, matrimony vine, hawthorn, lophatherum gracile and taurine, vitamin are prepared from jointly; major part composition is all natural food materials; can play good antifatigue, anti-oxidant, improve effect of body energy; can't have side effects, harm can not be brought to health.
Technical scheme provided by the invention: a kind of wheat peptide anti-fatigue health drink, formulated by the raw material of substance of following percentage by weight: wheat oligopeptide 0.5-5%, sesame protein peptide 0.5-5%, sugar or honey 5-12%, matrimony vine 1-5%, hawthorn 1-5%, honeysuckle 0.2-2%, lophatherum gracile 0.5-5%, taurine 0-0.06%, vitamin 0.025-0.05%, Juice or fruit vegetable powder 5-15%, citric acid 0.1%-0.3%, all the other are pure water;
Wherein said wheat oligopeptide is the oligopeptide that the molecular weight decomposed from Gluten is less than or equal to 1000Da, and this wheat oligopeptide middle-molecular-weihydroxyethyl is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%;
Described sesame protein peptide is the oligopeptide that the molecular weight decomposed from sesame seed meal is less than or equal to 1000Da, and the low peptide middle-molecular-weihydroxyethyl of this sesame protein is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%;
Described vitamin is composited according to following weight ratio by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 250-500:2-3:2-3:0.4-1.6;
Described Juice can select in pear juice, peach juice, pineapple juice, grape juice, wolfberry juice, Cranberry Juice Cocktail, blueberry juice, Cili Juice any one or a few, described fruit vegetable powder can select yam flour, spinach powder, celery powder, carrot meal wherein one or more.
Another kind of technical scheme provided by the invention:
A preparation method for wheat peptide anti-fatigue health drink, is characterized in that concrete steps are as follows:
(1) wheat oligopeptide is prepared
A. be raw material with Gluten, pure water is added by weight 1:2-6, add the food-grade reducing agent of the 0.01%-0.05% of liquid gross weight afterwards, re-use ultrasonic wave or microwave or the two series connection Combined Treatment 10 seconds-15 minutes, during the two series connection Combined Treatment, the time of ultrasonic wave and microwave treatment is equal, and wherein every microwave and ultrasound power needed for kg feed material is 0.2-5.0kw, the temperature of microwave is 60 DEG C-80 DEG C, and hyperacoustic temperature is 20 DEG C-60 DEG C; Described food-grade reducing agent comprises: vitamin C, Cys or L-cysteine hydrochloride, sodium sulfite, sodium hydrogensulfite, Hydros, potassium metabisulfite, one or more in sodium pyrosulfite.
B. the raw material after process in a is adopted colloid mill micronization processes further, then supplement the 3-6 pure water doubly adding former Gluten weight, 85-121 DEG C of process cools after 10 seconds-15 minutes and forms material liquid;
C. by material liquid cooled in step b, carry out twice enzyme hydrolysis, first time enzyme hydrolysis adds protease, and enzyme concentration is the 1%-6% of substrate protein white matter quality, and the condition that first time is hydrolyzed is: time 0.5-3 hour, and temperature 30-60 DEG C, pH are 2-9.0; After first time hydrolysis terminates, food flavor enzyme and neutral proteinase is added in hydrolyzate, or food flavor enzyme and acid protease, enzyme concentration is the 1%-5% of substrate protein white matter quality, the mixed proportion of two kinds of enzymes is 1-3:3-1, the condition of second time hydrolysis: the time is 1-3 hour, temperature 30-60 DEG C, pH2-8.0; Second time has been hydrolyzed rear centrifugation and has gone out hydrolyzate;
D. by the hydrolyzate of centrifugation in step c after micro porous filtration, molecule interception is adopted to be that 2 kinds of milipore filters of 3000-4000Da and 200-500Da are removed molecular weight respectively and are greater than the micromolecular composition that the large molecule of 1000Da and molecular weight be less than 200-500Da, further employing molecule interception concentrates in the NF membrane of 200-300Da, adopt cationic ion-exchange resin and anion exchange resin absorption afterwards, again by obtaining water-soluble and raciness after freeze drying or spraying dry, and molecular weight is less than or equal to the wheat oligopeptide of 1000Da, this wheat oligopeptide middle-molecular-weihydroxyethyl is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%,
(2) sesame protein peptide is prepared, take sesame seed meal as raw material, the 3-8 times of pure water adding sesame seed meal weight is uniformly dispersed, add protease afterwards and carry out first time hydrolysis, enzyme concentration is the 1%-6% of substrate protein white matter quality, and the condition that first time is hydrolyzed is: time 0.5-4 hour, temperature 30-60 DEG C, pH is 2-9.0, and enzyme concentration is the 1%-6% of substrate protein white matter, 30 minutes to 4 hours time, after first time, hydrolysis terminated, in hydrolyzate, add food flavor enzyme and neutral proteinase, or food flavor enzyme and acid protease, enzyme concentration is the 1%-5% of substrate protein white matter, and the ratio of two kinds of enzymes is 1-3:3-1, continues hydrolysis 1-3 hour, hydrolyzate is gone out afterwards through centrifugation, and after micro porous filtration, molecule interception is adopted to be that 2 kinds of milipore filters of 3000-5000Da and 200-500Da are removed molecular weight respectively and are greater than the micromolecular composition that the large molecule of 1000Da and molecular weight be less than 200-500Da, further employing molecule interception concentrates in the NF membrane of 200-300Da, molecular weight is obtained for being less than or equal to 1000Da again by drying after hybrid resin absorption, and water-soluble and sapid sesame protein peptide, this sesame protein peptide middle-molecular-weihydroxyethyl is that the oligopeptide content of 300-1000Da accounts for 90%-95%, described hybrid resin is mixed according to the ratio of mass ratio 1-3:3-1 by 732 cationic ion-exchange resins and XDA-200 macroreticular resin,
(3) take matrimony vine, hawthorn, honeysuckle, lophatherum gracile according to the mass percent in formula, and after being mixed, add gross weight 3-10 water doubly, and filtration removal filter residue after infusion 1-3 hour is boiled in heating, is cooled by mixed liquor for subsequent use;
(4) following material is prepared according to the mass percent in formula: the wheat oligopeptide of preparation in the step (1) of 0.5-5%, the sesame protein peptide of preparation in the step (2) of 0.5-5%, 5-12% sugar or honey 5-12%, taurine 0-0.06%, the Juice of vitamin 0.025-0.05% and 5-15% or fruit vegetable powder, citric acid 0.1%-0.3%, then with ready matrimony vine in step (3), hawthorn, honeysuckle, lophatherum gracile mixture mixes, after pure water constant volume, sheet frame or bag filter is adopted to filter, and under the condition of 85-135 DEG C, adopt chip or tubular type or high-temperature instantaneous sterilization after 6 seconds-30 seconds, filling, at the Water Under bath sterilization 15-45 minute of 85 DEG C-98 DEG C after capping, cooling, be prepared into antifatigue wheat peptide drink, wherein, described vitamin is composited according to part by weight by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 250-500:2-3:2-3:0.4-1.6, described Juice can select in pear juice, peach juice, pineapple juice, grape juice, wolfberry juice, Cranberry Juice Cocktail, blueberry juice, Cili Juice any one or a few, described fruit vegetable powder can select yam flour, spinach powder, celery powder, carrot meal wherein one or more.
The wheat peptide anti-fatigue health drink of preparation adopts vial or plastic bottle or metal can filling in described step (4), cooled by cool water shower, and cleaned by body, dry up rear labeling packaging.
In the step c neutralization procedure (2) of step (1), first time enzyme hydrolysis protease comprises one or more in alkali protease, neutral proteinase, acid protease, papain, bromelain, ficin, food flavor enzyme etc.
Beneficial effect of the present invention:
1. in the present invention, wheat peptide take Gluten as raw material, Gluten take wheat as the flaxen a kind of natural grain albumen of one that Raw material processing extracts, main component is glutenin and gliadin, accounts for wheat gluten about 80%, 15 seed amino acids containing needed by human; The wheat peptide being Raw material processing by Gluten has the effect suppressing cholesterol to rise, can also rent insulin chalk, and the blood sugar of mediator, improves diabetic symptom; Containing glutamine in it, the glu famine stability of specific ionization is high, effectively can not only regulate nerve, also can make special dietary material during intestinal dysfunction, than direct wheat be the anti-fatigue performance of the wheat peptide of Raw material processing better, also there is anti-oxidant, raising body's immunity, the effect such as hypotensive;
2. the present invention also added sesame peptide, the raw materials of sesame peptide adopts sesame seed meal, a large amount of discarded sesame seed meals can be used, it is with low cost, and sesame peptide contains the arginine than high level, arginine, as nitric oxide production precursor substance, has the effects such as hemangiectasis, antifatigue, enhancing human activity; By sesame peptide and wheat peptide with the use of, to improving the health, hypotensive, antifatigue has better effect, can well enhanced machine vitality of subject;
3. the present invention with the addition of matrimony vine, hawthorn, honeysuckle, these natural plants of lophatherum gracile, and wherein contained in honeysuckle chlorogenic acid can cause central nervous excitation, and its action intensity is 1/6 of caffeine, has effect of antifatigue; In matrimony vine, topmost active component is LBP-X, and LBP-X is a kind of water-soluble polysaccharide, and be topmost active component in matrimony vine, relative molecular mass is 68-200, has Promote immunity, and antifatigue is anti-ageing, antitumor, the multiple actions such as radioresistance; Main component in hawthorn is Flavonoid substances, wherein containing flavonoid glycoside, flavonols and glycoside thereof, dioxygen flavonoid glycoside, polymerization flavonoids; Flavonoid substances can block, and reduces the formation of free radical, strengthens immunity of organisms, has anti-ageing, antifatigue, effect such as anticancer grade; Active ingredient in lophatherum gracile is the trace elements such as flavone compound, phenolic acid compound, amino acid, manganese and zinc, active oxygen radical in these active ingredient energy purged body, the Antioxidant Enzymes of induction organism inside is active, effectively can strengthen body resisting fatigue ability, delay senility;
4. also added taurine and vitamin in the present invention, taurine (taurine, Tau) chemical name Tau, molecular formula is: H 2n-CH 2-CH 2-SO 2h; that human development grows necessary nutrient; and growing of central nervous system can be promoted; the taurine (200mg/L) of optimal dose can increase the enzymatic activity of SOD, GSH-Px in nerve cell; increase the function of scavenging free radicals; and maintain the mobility of neuron membrane; in addition; taurine can also suppress cardiac muscle cell apoptosis, the cardiopathic generation of expanding property of prevention, protection blood vessel endothelium; reduce blood fat and cholesterol level; and can pancreatic cell be protected, increase the secretion of insulin, thus improve diabetes.Vitamin is composited by vitamin C, vitamin B1, vitamin B2, vitamin B6, wherein vitamin C can promote the biosynthesis of ossein, be beneficial to healing sooner of tissue injury mouth, prevent arthralgia, pain in waist and lower extremities, promote the metabolism of tyrosine and tryptophan in amino acid, extend the human body life-span, strengthen the human body anti-stress ability of environment and immunity to external world, improve the utilization of iron, calcium and folic acid; Vitamin B1 is a kind of B family vitamin be combined into by pyrimidine ring and thiazole ring, have and promote to grow up, help digest, particularly the digestion of carbohydrate improves mental status, maintain nerve fiber, muscle, cardiomotility normal, and the nerve pain after as dental operation can be alleviated; Vitamin B2 can participate in biological oxidation and energetic supersession in body, can improve the utilization rate of human body to protein, enhancing development, participates in Cobastab 6metabolism, relevant with mobilization with the absorption of body iron, storage, also there is antioxidation activity, may be relevant with flavo-enzyme-glutathione reductase.
5. also added Juice or fruit vegetable powder in the present invention, Juice or fruit vegetable powder are made up of fruits and vegetables, wherein containing the abundant composition such as vitamin, polyphenol.Wherein as blueberry, except the sugar containing routine, acid, protein, fat, amino acid, food fiber and VC, also be rich in VII, VA, VB, pectin, SOD, Arbutin, anthocyanin, niacin, the mineral element such as rare special composition and abundant potassium, iron, zinc, calcium, manganese, germanium, copper in other fruit such as carotenoid, can effectively alleviate or fatigue-relieving.
Present invention incorporates wheat peptide and sesame peptide; coordinate some natural nutrient substances such as matrimony vine, hawthorn, honeysuckle, lophatherum gracile, fruits and vegetables; and add some nutritional labelings such as vitamin and taurine; the beverage prepared is except the function of antifatigue; also there is plurality of health care functions; and delicious taste, has no side effect, it is a kind of desirable health drink.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment one: described a kind of wheat peptide anti-fatigue health drink, its preparation method is as follows:
(1) composition of raw materials is prepared according to the material of following percentage by weight: molecular weight is mainly 300 ~ 1000 daltonian wheat oligopeptides 3%, molecular weight is mainly 300 ~ 1000 daltonian sesame protein peptides 2%, sugar or honey 8%, matrimony vine 2%, hawthorn 2%, honeysuckle 1%, lophatherum gracile 1%, taurine 0.025%, vitamin 0.025%, pineapple juice 5%, citric acid 0.15%, all the other are pure water; Described vitamin is composited according to following part by weight by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 300:2:2:1;
(2) first will add the water of gross weight 5 times after the matrimony vine prepared in step (1), hawthorn, honeysuckle, lophatherum gracile mixing, and heating is boiled infusion and is crossed after 1.5 hours and filter to remove filter residue, is cooled by mixed liquor for subsequent use;
(3) other material in step (1) to be added in step (3) in ready matrimony vine, hawthorn, honeysuckle, lophatherum gracile mixed liquor, then sheet frame or bag filter is adopted to filter, and under the condition of 121 DEG C, adopt the sterilization of ultra high temperature short time sterilization equipment after about 10 seconds, adopt vial filling;
(4) after the antifatigue wheat peptide drink after filling being carried out water dip sterilization 30-45 minute under the condition of 85-98 DEG C (reasonable temperature is 95 DEG C), cooled by cool water shower, and body is cleaned, dry up rear labeling packaging.
Embodiment two: a kind of wheat peptide anti-fatigue health drink, its preparation method is as follows:
(1) composition of raw materials is prepared according to the material of following percentage by weight: molecular weight is mainly 300 ~ 1000 daltonian wheat oligopeptides 5%, molecular weight is mainly 300 ~ 1000 daltonian sesame protein peptides 4%, sugar or honey 6%, matrimony vine 3%, hawthorn 3%, honeysuckle 1.5%, lophatherum gracile 1.5%, taurine 0.05%, vitamin 0.05%, grape juice 8%, citric acid 0.20%, all the other are pure water; Described vitamin is composited according to following part by weight by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 350:2.5:2.5:1.2;
(2) first will add the water of gross weight 7 times after the matrimony vine prepared in step (1), hawthorn, honeysuckle, lophatherum gracile mixing, and heating is crossed after boiling infusion 2-3 hour and is filtered to remove filter residue, is cooled by mixed liquor for subsequent use;
(3) other material in step (1) to be added in step (3) in ready matrimony vine, hawthorn, honeysuckle, lophatherum gracile mixed liquor, then sheet frame or bag filter is adopted to filter, and under the condition of 95 DEG C, adopt the sterilization of tubular type disinfection equipment after about 15 seconds, adopt vial filling.；
(4) the antifatigue wheat peptide drink after filling is carried out water dip sterilization after about 35 minutes under the condition of 85-98 DEG C (preferably temperature is 96 DEG C), cooled by cool water shower, and body is cleaned, dry up rear labeling packaging.
Embodiment three: a kind of wheat peptide anti-fatigue health drink, its preparation method is as follows:
(1) prepare composition of raw materials molecular weight according to the material of following percentage by weight and be mainly 300 ~ 1000 daltonian wheat oligopeptides 4%, molecular weight is mainly 300 ~ 1000 daltonian sesame protein peptides 3%, sugar or honey 7%, matrimony vine 4%, hawthorn 4%, honeysuckle 0.8%, lophatherum gracile 0.8%, taurine 0.03%, vitamin 0.03%, yam flour 10%, citric acid 0.25%, all the other are pure water; Described vitamin is composited according to following weight ratio by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 400:3:3:1.4;
(2) first will add the water of gross weight 8 times after the matrimony vine prepared in step (1), hawthorn, honeysuckle, lophatherum gracile mixing, and heating is boiled infusion and is crossed after 2.5 hours and filter to remove filter residue, is cooled by mixed liquor for subsequent use;
(3) other material in step (1) to be added in step (3) in ready matrimony vine, hawthorn, honeysuckle, lophatherum gracile mixed liquor, then sheet frame or bag filter is adopted to filter, and under the condition of 98 DEG C, adopt the sterilization of chip disinfection equipment after about 20 seconds, vial is filling;
(4) after the antifatigue wheat peptide drink after filling being carried out water dip sterilization 30-45 minute under the condition of 85-98 DEG C (optimum temperature is 85 DEG C), cooled by cool water shower, and body is cleaned, dry up rear labeling packaging.
Embodiment four: a kind of wheat peptide anti-fatigue health drink, its preparation method is as follows:
(1) composition of raw materials is prepared according to the material of following percentage by weight: molecular weight is mainly 300 ~ 1000 daltonian wheat oligopeptides 5%, molecular weight is mainly 300 ~ 1000 daltonian sesame protein peptides 4%, sugar or honey 10%, matrimony vine 4.5%, hawthorn 4.5%, honeysuckle 1.8%, lophatherum gracile 3%, taurine 0.04%, vitamin 0.04%, celery powder 12%, citric acid 0.18%, all the other are pure water; Described vitamin is composited according to following weight ratio by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 300:3:3:1.5;
(2) first will add the water of gross weight 9 times after the matrimony vine prepared in step (1), hawthorn, honeysuckle, lophatherum gracile mixing, and heating is boiled infusion and is crossed after 3 hours and filter to remove filter residue, is cooled by mixed liquor for subsequent use;
(3) other material in step (1) to be added in step (3) in ready matrimony vine, hawthorn, honeysuckle, lophatherum gracile mixed liquor, then sheet frame or bag filter is adopted to filter, and under the condition of 130 DEG C, after adopting ultra high temperature short time sterilization equipment sterilization 6-10 second, plastic bottle filling;
(4) the antifatigue wheat peptide drink after filling is carried out water dip sterilization after 30 minutes under the condition of 85-98 DEG C (optimum temperature is 94 DEG C), cooled by cool water shower, and body is cleaned, dry up rear labeling packaging.
Embodiment five: a kind of wheat peptide anti-fatigue health drink, its preparation method is as follows:
(1) composition of raw materials is prepared according to the material of following percentage by weight: molecular weight is mainly 300 ~ 1000 daltonian wheat oligopeptides 5%, molecular weight is mainly 300 ~ 1000 daltonian sesame protein peptides 2%, sugar or honey 10%, matrimony vine 3.5%, hawthorn 3.5%, honeysuckle 1.8%, lophatherum gracile 4%, taurine 0.03%, vitamin 0.03%, blueberry juice 10%, citric acid 0.26%, all the other are pure water; Described vitamin is composited according to part by weight by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 450:2.5:2.5:1.2;
(2) first will add the water of gross weight 4 times after the matrimony vine prepared in step (1), hawthorn, honeysuckle, lophatherum gracile mixing, and heating is boiled infusion and is crossed after 1.2 hours and filter to remove filter residue, is cooled by mixed liquor for subsequent use;
(3) other material in step (1) to be added in step (3) in ready matrimony vine, hawthorn, honeysuckle, lophatherum gracile mixed liquor, then sheet frame or bag filter is adopted to filter, and under the condition of 90 DEG C, adopt the sterilization of tubular type disinfection equipment after 25 seconds, adopt metal can filling;
(4) the antifatigue wheat peptide drink after filling is carried out water dip sterilization after 35 minutes under the condition of 85-98 DEG C (optimum temperature 98 DEG C), cooled by cool water shower, and can body is cleaned, dry up rear labeling packaging.
The preparation method of the wheat oligopeptide in embodiment one to embodiment five is as follows:
A. be raw material with Gluten, pure water is added by weight 1:2-6, add the food-grade reducing agent of the 0.01%-0.05% of liquid gross weight afterwards, can be used alone ultrasonic wave, or be used alone microwave treatment, or adopt ultrasonic wave and microwave combined process, the time of process is 10 seconds-15 minutes, when adopting ultrasonic wave and microwave combined process, the time of both each half, microwave during process needed for every kg feed material or hyperacoustic power are 0.2-5.0kw, and wherein the temperature of microwave is 60 DEG C-80 DEG C, and hyperacoustic temperature is 20 DEG C-60 DEG C; Described food-grade reducing agent comprises: vitamin C, Cys or L-cysteine hydrochloride, sodium sulfite, sodium hydrogensulfite, Hydros, potassium metabisulfite, one or more in sodium pyrosulfite;
B. the raw material after process in a is adopted colloid mill micronization processes further, then supplement the 3-6 pure water doubly adding raw material Gluten weight, 85-121 DEG C of process cools after 10 seconds-15 minutes and forms material liquid;
C. by material liquid cooled in step b, carry out twice enzyme hydrolysis, first time enzyme hydrolysis adds protease (described protease comprises alkali protease, neutral proteinase, acid protease, papain, bromelain, ficin, food flavor enzyme), enzyme concentration is the 1%-6% of substrate protein white matter quality, the quality of substrate protein white matter calculates after adopting Kjeldahl's method to detect to protein content in raw material Gluten, the condition that first time is hydrolyzed is: time 0.5-3 hour, temperature 30-60 DEG C, pH are 2-9.0; After first time hydrolysis terminates, food flavor enzyme and neutral proteinase is added in hydrolyzate, or food flavor enzyme and acid protease, enzyme concentration is the 1%-5% of substrate protein white matter quality, the mixed proportion of two kinds of enzymes is 1-3:3-1, the condition of second time hydrolysis: the time is 1-3 hour, temperature 30-60 DEG C, pH2-8.0; Second time has been hydrolyzed rear centrifugation and has gone out hydrolyzate;
D. by the hydrolyzate of centrifugation in step c after micro porous filtration, molecule interception is adopted to be that 2 kinds of milipore filters of 3000-4000Da and 200-500Da are removed molecular weight respectively and are greater than the micromolecular impurity that the large molecule of 1000Da and molecular weight be less than 200-500Da, further employing molecule interception concentrates in the NF membrane of 200-300Da, adopt cationic ion-exchange resin and anion exchange resin absorption afterwards, again by obtaining water-soluble and raciness after freeze drying or spraying dry, and molecular weight is less than or equal to the wheat oligopeptide of 1000Da, this wheat oligopeptide middle-molecular-weihydroxyethyl is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%,
The preparation method of the sesame protein peptide in embodiment one to embodiment five is as follows: take sesame seed meal as raw material, the 3-8 times of pure water adding sesame seed meal weight is uniformly dispersed, add protease afterwards and carry out first time hydrolysis, enzyme concentration is the 1%-6% of substrate protein white matter quality, the condition that first time is hydrolyzed is: time 0.5-4 hour, temperature 30-60 DEG C, pH is 2-9.0, enzyme concentration is the 1%-6% of substrate protein white matter, the quality of substrate protein white matter calculates after adopting Kjeldahl's method to detect to protein content in raw material sesame seed meal, 30 minutes to 4 hours time, described protease comprises alkali protease, neutral proteinase, acid protease, papain, bromelain, ficin, food flavor enzyme, after first time, hydrolysis terminated, in hydrolyzate, add food flavor enzyme and neutral proteinase, or food flavor enzyme and acid protease, enzyme concentration is the 1%-5% of substrate protein white matter, and the ratio of two kinds of enzymes is 1-3:3-1, continues hydrolysis 1-3 hour, hydrolyzate is gone out afterwards through centrifugation, and after micro porous filtration, molecule interception is that 2 kinds of milipore filters of 3000-5000Da and 200-500Da are removed molecular weight respectively and are greater than the micromolecular impurity that the large molecule of 1000Da and molecular weight be less than 200-500Da, further employing molecule interception concentrates in the NF membrane of 200-300Da, molecular weight is obtained for being less than or equal to 1000Da again by drying after hybrid resin absorption, and water-soluble and sapid sesame protein peptide, this sesame protein peptide middle-molecular-weihydroxyethyl is that the oligopeptide content of 300-1000Da accounts for 90%-95%, described hybrid resin is mixed according to the ratio of mass ratio 1-3:3-1 by 732 cationic ion-exchange resins and XDA-200 macroreticular resin.
The wheat peptide drink that employing above-described embodiment is prepared, except the function of antifatigue, also has plurality of health care functions, and delicious taste, having no side effect, is a kind of desirable health drink.

Claims (3)

1. a preparation method for wheat peptide anti-fatigue health drink product, formulated by the raw material of substance of following percentage by weight: wheat oligopeptide 0.5-5%, sesame protein peptide 0.5-5%, sugar or honey 5-12%, matrimony vine 1-5%, hawthorn 1-5%, honeysuckle 0.2-2%, lophatherum gracile 0.5-5%, taurine 0-0.06%, vitamin 0.025-0.05%, Juice or fruit vegetable powder 5-15%, citric acid 0.1%-0.3%, all the other are pure water;
Wherein said wheat oligopeptide is the oligopeptide that the molecular weight decomposed from Gluten is less than or equal to 1000Da, and this wheat oligopeptide middle-molecular-weihydroxyethyl is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%;
Described sesame protein peptide is the oligopeptide that the molecular weight decomposed from sesame seed meal is less than or equal to 1000Da, and the low peptide middle-molecular-weihydroxyethyl of this sesame protein is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%;
Described vitamin is composited according to following weight ratio by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 250-500:2-3:2-3:0.4-1.6;
Described Juice select in pear juice, peach juice, pineapple juice, grape juice, wolfberry juice, Cranberry Juice Cocktail, blueberry juice, Cili Juice any one or a few, described fruit vegetable powder selects yam flour, spinach powder, celery powder, carrot meal wherein one or more;
Concrete steps are as follows:
(1) wheat oligopeptide is prepared
A. be raw material with Gluten, pure water is added by weight 1:2-6, add the food-grade reducing agent of the 0.01%-0.05% of liquid gross weight afterwards, re-use ultrasonic wave or microwave or the two series connection Combined Treatment, wherein every microwave and ultrasound power needed for kg feed material is 0.2-5.0kw, the temperature of microwave is 60 DEG C-80 DEG C, and hyperacoustic temperature is 20 DEG C-60 DEG C; Described food-grade reducing agent comprises: vitamin C, Cys or L-cysteine hydrochloride, sodium sulfite, sodium hydrogensulfite, Hydros, potassium metabisulfite, one or more in sodium pyrosulfite.
B. the raw material after process in step a is adopted colloid mill micronization processes further, then supplement the 3-6 pure water doubly adding former Gluten weight, 85-121 DEG C of process cools after 10 seconds-15 minutes and forms material liquid;
C. by material liquid cooled in step b, carry out twice enzyme hydrolysis, first time enzyme hydrolysis adds protease, and enzyme concentration is the 1%-6% of substrate protein white matter quality, and the condition that first time is hydrolyzed is: time 0.5-3 hour, and temperature 30-60 DEG C, pH are 2-9.0; After first time hydrolysis terminates, food flavor enzyme and neutral proteinase is added in hydrolyzate, or food flavor enzyme and acid protease, enzyme concentration is the 1%-5% of substrate protein white matter quality, the mixed proportion of two kinds of enzymes is 1-3:3-1, the condition of second time hydrolysis: the time is 1-3 hour, temperature 30-60 DEG C, pH 2-8.0; Second time has been hydrolyzed rear centrifugation and has gone out hydrolyzate;
D. by the hydrolyzate of centrifugation in step c after micro porous filtration, employing molecule interception is that 2 kinds of milipore filters of 3000-4000Da and 200-500Da remove large molecule and Small molecular composition respectively, molecule interception is adopted to concentrate in the NF membrane of 200-300Da more further, adopt cationic ion-exchange resin and anion exchange resin absorption afterwards, again by obtaining water-soluble and raciness after freeze drying or spraying dry, and molecular weight is less than or equal to the wheat oligopeptide of 1000Da, this wheat oligopeptide middle-molecular-weihydroxyethyl is that the oligopeptide of 300 ~ 1000Da accounts for 90%-95%;
(2) sesame protein peptide is prepared, take sesame seed meal as raw material, the 3-8 times of pure water adding sesame seed meal weight is uniformly dispersed, add protease afterwards and carry out first time hydrolysis, enzyme concentration is the 1%-6% of substrate protein white matter quality, and the condition that first time is hydrolyzed is: time 0.5-4 hour, temperature 30-60 DEG C, pH is 2-9.0, and enzyme concentration is the 1%-6% of substrate protein white matter, 30 minutes to 4 hours time, after first time, hydrolysis terminated, in hydrolyzate, add food flavor enzyme and neutral proteinase, or food flavor enzyme and acid protease, enzyme concentration is the 1%-5% of substrate protein white matter, and the ratio of two kinds of enzymes is 1-3:3-1, continues hydrolysis 1-3 hour, hydrolyzate is gone out afterwards through centrifugation, and after micro porous filtration, employing molecule interception is that 2 kinds of milipore filters of 3000-5000Da and 200-500Da remove large molecule and Small molecular composition respectively, further employing molecule interception concentrates in the NF membrane of 200-300Da, molecular weight is obtained for being less than or equal to 1000Da again by drying after hybrid resin absorption, and water-soluble and sapid sesame protein peptide, this sesame protein peptide middle-molecular-weihydroxyethyl is the oligopeptide content of 300-1000Da is 90%-95%, described hybrid resin is mixed according to the ratio of mass ratio 1-3:3-1 by 732 cationic ion-exchange resins and XDA-200 macroreticular resin,
(3) take matrimony vine, hawthorn, honeysuckle, lophatherum gracile according to the mass percent in formula, and after being mixed, add gross weight 3-10 water doubly, and filtration after infusion 1-3 hour is boiled in heating, is cooled by mixed liquor for subsequent use after removing filter residue;
(4) following material is prepared according to the mass percent in formula: the wheat oligopeptide of preparation in the step (1) of 0.5-5%, the sesame protein peptide of preparation in the step (2) of 0.5-5%, 5-12% sugar or honey 5-12%, taurine 0-0.06%, vitamin 0.025-0.05%, Juice or fruit vegetable powder 5-15%, citric acid 0.1%-0.3%, then with ready matrimony vine in step (3), hawthorn, honeysuckle, lophatherum gracile mixture mixes, use pure water constant volume, sheet frame or bag filter is adopted to filter, under the condition of 85-135 DEG C, adopt chip or tubular type or high temperature sterilization after 6 seconds-30 seconds, filling and capping, cool after 15-45 minute be prepared into antifatigue wheat peptide drink the Water Under bath sterilization of 85 DEG C-98 DEG C again, wherein said vitamin is composited according to part by weight by vitamin C, vitamin B1, vitamin B2 and vitamin B6: 250-500:2-3:2-3:0.4-1.6, described Juice can select in pear juice, peach juice, pineapple juice, grape juice, wolfberry juice, Cranberry Juice Cocktail, blueberry juice, Cili Juice any one or a few, described fruit vegetable powder can select yam flour, spinach powder, celery powder, carrot meal wherein one or more.
2. the preparation method of wheat peptide anti-fatigue health drink product according to claim 1, it is characterized in that: in described step (4), the wheat peptide anti-fatigue health drink of preparation adopts vial or plastic bottle or metal can filling, cooled by cool water shower, and by body clean, dry up rear labeling packaging.
3. the preparation method of wheat peptide anti-fatigue health drink product according to claim 1, is characterized in that: in the step c neutralization procedure (2) of step (1), first time enzyme hydrolysis protease comprises one or more in alkali protease, neutral proteinase, acid protease, papain, bromelain, ficin, food flavor enzyme.
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