CN103652381B - The composite immune reinforcing agent of the young children's ginseng of a kind of stichopus japonicus - Google Patents
The composite immune reinforcing agent of the young children's ginseng of a kind of stichopus japonicus Download PDFInfo
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- CN103652381B CN103652381B CN201210328024.0A CN201210328024A CN103652381B CN 103652381 B CN103652381 B CN 103652381B CN 201210328024 A CN201210328024 A CN 201210328024A CN 103652381 B CN103652381 B CN 103652381B
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Abstract
The present invention relates to composite immune reinforcing agent of the young children's ginseng of a kind of stichopus japonicus and preparation method thereof, in mass fraction, comprise astragalus polyose 15-20, Codonopsis pilosula polysaccharide 10-15, Radix Isatidis 10-20, Herba Andrographitis 5-10, honeysuckle 5-10, vitamin C 0.5-5, vitamin e1-6, secondary powder 20-40; Above-mentioned raw materials is pulverized through micronizer, mixes after quantitative according to proportioning after 100 mesh sieves; In feed according to mass ratio be 0.2-0.5% ratio add.Composite immune reinforcing agent provided by the invention, can significantly improve the non-specific immunity of the young children's ginseng of stichopus japonicus, strengthen the performance of body.
Description
Technical field
The present invention relates to a kind of feed addictive, particularly young young composite immune reinforcing agent of joining of a kind of stichopus japonicus and preparation method thereof.
Background technology
Sea cucumber has higher edible medical value and economic worth, by the precious marine product with special nourishing and nutrition and health care multi-efficiency extensively admitted.Along with people are to the increase of stichopus japonicus demand, adopt and catch intensity and increase gradually, cause China's Holothurian Resources amount sharply to decline.Meanwhile, sea cucumber propagates the continuous expansion of scale artificially, and sea cucumber kind matter degradation phenomena is on the rise, and the farming disease harms such as to be on the rise at the series of problems, seriously governs the development of sea cucumber industry.
Immunopotentiator refers to by different modes, has and promotes or bring out host to produce defense reaction, reach the material strengthening immunity of organisms.Mainly act on the nospecific immunity of aquatic products invertebrate, activate the immune defense system of body, thus reach the object that develop immunitypty improves its resistance against diseases.Immunopotentiator has safe ready, noresidue and sphere of action widely feature, in resistance disease and raising immunity of organisms, be obviously better than vaccine and antibiotic.In current aquatic animal disease prevention and cure, antibiotic still plays a part main, but considers its resistance to the action of a drug and residue problem, antibiotic day by day eliminate by people, instead will be immunopotentiator and vaccine etc., vaccine owing to there being specificity, therefore, in disease preventing and treating, effect is good not as the broad spectrum activity of immunopotentiator, the using method of vaccine in addition and level of processing limited, therefore, current immunopotentiator is subject to the absolute favor of people.Aquaculture with " anti-overweight control " for principle, and the effect of immunopotentiator exactly focus on anti-on, and its curative effect also highly significant in controlling.In addition, immunopotentiator uses jointly as adjuvant and vaccine, more can strengthen the curative effect of vaccine, and therefore, its development field is widened further.Along with the using method to vaccine reinforcing agent, dosage and time limit and to the research of aquatic livestock immunologic mechanism further deeply, immunopotentiator will become control aquatic animal disease, improves the very effective approach of immunity.At present, by multi-source, multipath mode develops low toxicity, efficiently, quick-acting, and Novel immune reinforcing agent has become the main trend of development longer.
Summary of the invention
Technical problem solved by the invention is to provide young young ginseng composite immune reinforcing agent of a kind of stichopus japonicus and preparation method thereof, it is added in feed to feed and raises young children's ginseng, the anti-stress ability of young children's ginseng can be improved, reduce the damage that stress bring, strengthen the immunocompetence of body, improve its survival rate.
Improve a composite immune reinforcing agent for stichopus japonicus young children's ginseng anti-stress ability, be made up of the material of following parts by weight:
Astragalus polyose: 15-20;
Codonopsis pilosula polysaccharide: 10-15;
Radix Isatidis: 10-20;
Herba Andrographitis: 5-10;
Honeysuckle (also known as honeysuckle): 5-10;
Vitamin C: 0.5-5;
Vitamin E: 1-6;
Secondary powder: 20-40.
Described astragalus polyose be the dry root of legume astragalus mongolicus or Astragalus membranacus through extracting, the water-soluble heteroglycan of concentrated, purifying.Faint yellow, powder is fine and smooth, even free from admixture.Wherein astragalus polyose (Polysacchrides) content >=50%.
The Powdered polysaccharide compound that described Codonopsis pilosula polysaccharide is Campanulaceae Radix Codonopsis, element spends the dry root of Radix Codonopsis or radix codonpsis tangshen to obtain after water extract-alcohol precipitation, wherein Codonopsis pilosula polysaccharide content >=30%.
Described powder is the part between flour and wheat bran, and flour mill can buy.
Composite immune reinforcing agent of the present invention, it is preferably made up of the material of following parts by weight:
Astragalus polyose: 17;
Codonopsis pilosula polysaccharide: 15;
Radix Isatidis: 13;
Herba Andrographitis: 8;
Honeysuckle: 7;
Vitamin C: 3.5;
Vitamin E: 2.5;
Secondary powder: 33.
The preparation method of above-mentioned composite immune reinforcing agent, described material is pulverized through micronizer, mixes after quantitative according to proportioning after 100 mesh sieves.
Above-mentioned composite immune reinforcing agent, it be added in feed to feed and raise young children's ginseng, can improve the anti-stress ability of young children's ginseng, the ratio being 0.2-0.5% according to mass ratio in feed adds described immunopotentiator.
Composite immune reinforcing agent provided by the invention, can significantly improve the non-specific immunity of the young children's ginseng of stichopus japonicus, strengthen the performance of body.
Detailed description of the invention
For further illustrating the present invention, illustrate with the following Examples:
The astragalus polyose adopted in following examples purchased from Shaanxi Yong Yuan Bioisystech Co., Ltd, production code member: YYS-015.Product specification: astragalus polyose content 50%(mass concentration); 5:1(concentrates volume ratio).
Codonopsis pilosula polysaccharide purchased from Shaanxi Yong Yuan Bioisystech Co., Ltd, production code member: YYR-040.Product specification: Codonopsis pilosula polysaccharide content 30%(mass concentration); 10:1(concentrates volume ratio).
Embodiment 1
Take astragalus polyose 17kg, Codonopsis pilosula polysaccharide 15kg, Radix Isatidis 13kg, Herba Andrographitis 8kg, honeysuckle 7kg, vitamin C 3.5kg, vitamin E2 .5kg, secondary powder 33kg, pulverize through micronizer, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then be added in the feed of stichopus japonicus according to 0.2% ratio.
Embodiment 2
Take astragalus polyose 15kg, Codonopsis pilosula polysaccharide 15kg, Radix Isatidis 20kg, Herba Andrographitis 5kg, honeysuckle 10kg, Catergen kg, vitamin E 5kg, secondary powder 33kg, pulverize through micronizer, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then be added in the feed of stichopus japonicus according to 0.3% ratio.
Embodiment 3
Take astragalus polyose 20kg, Codonopsis pilosula polysaccharide 10kg, Radix Isatidis 15kg, Herba Andrographitis 10kg, honeysuckle 5kg, vitamin C 5kg, vitamin e1 kg, secondary powder 34kg, pulverize through micronizer, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then be added in the feed of stichopus japonicus according to 0.4% ratio.
Embodiment 4
Take astragalus polyose 19kg, Codonopsis pilosula polysaccharide 13kg, Radix Isatidis 17kg, Herba Andrographitis 6kg, honeysuckle 9kg, vitamin C 1kg, vitamin E 3kg, secondary powder 32kg, pulverize through micronizer, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then be added in the feed of stichopus japonicus according to 0.5% ratio.
Test example:
One, the raising of stichopus japonicus and management
Select the same a collection of stichopus japonicus hatched then as experiment ginseng, original body mass is 3.90 ± 0.84g.Setup Experiments 5 groups, is respectively control group, embodiment 1, embodiment 2, embodiment 3, embodiment 4.Wherein control group is thrown something and fed and is not added the apostichopus japonicus Selenka feed of immunopotentiator, and other embodiment groups with the addition of the immunopotentiator of 0.2-0.5% respectively on the basis of control group, and its composition and adding proportion are carried out according to embodiment 1-4.Often group 3 is parallel, and each parallel 30 stichopus japonicus, throw something and feed every day 1 time, Feeding time is 17:00, is satiated with food and throws something and feeds, and next day, 8:00 absorbed residual bait and ight soil.Continuous charge between feeding period, water temperature controls at 15 ± 0.5 DEG C, and salinity 32, PH7.8-8.2, dissolved oxygen is not less than 5mg/L.
Two, stichopus japonicus sample collection, analyze with calculating
Within 10th, 20,30,40 and 50, the various immune enzyme activity of stichopus japonicus and growing state is measured after throwing something and feeding.Calculate the specific growth rate of stichopus japonicus.Computing formula is as follows:
Survival rate (%)=100 × (Nt-No)/No
Rate of body weight gain (WG, %)=100 × (Wt-Wo)/Wo
Specific growth rate (SGR, %)=100 × (lnWt-lnWo)/t
Wherein, No with Nt is respectively each parallel stichopus japonicus initial head number and whole last head number, Wt and Wo is respectively original body mass and whole opisthosoma weight, and t is experiment number of days.
Get coelomic fluid, whole process is carried out on ice chest, prevents enzyme deactivation.Coelomic fluid is loaded in 1.5mL centrifuge tube, be temporarily kept in 4 DEG C of refrigerators.Centrifugal, 3000r/min, 4 DEG C of centrifugal 10min, get supernatant ,-80 DEG C of preservations.Its AKP, ACP, LSZ and SOD enzyme activity of absorbance measurement is measured with ELIASA.
SPSS11.5forWindows is adopted to carry out one-way analysis of variance to the data obtained.
Three, experimental result is in table 1 and table 2:
Table 1 throws something and feeds compound immunopotentiator for apostichopus japonicus to stichopus japonicus growth and the impact of survival rate
| Group | Survival rate % | Rate of body weight gain % | Specific growth rate % |
| Control group (A) | 97.12±0.83 | 2.56±0.78 | 0.02±0.01 |
| Embodiment 1 | 95.77±1.01 | 15.58±2.57 | 0.15±0.03 |
| Embodiment 2 | 98.85±0.86 | 14.42±1.84 | 0.10±0.05 |
| Embodiment 3 | 97.43±0.54 | 13.82±1.64 | 0.12±0.04 |
| Embodiment 4 | 98.50±0.47 | 15.21±2.75 | 0.14±0.02 |
Table 2 is thrown something and fed the impact of compound immunopotentiator for apostichopus japonicus on stichopus japonicus immuno-enzymatic vigor
| Group | AKP enzyme is lived | ACP enzyme is lived | LSZ enzyme is lived | SOD enzyme is lived |
| Control group (A) | 2.98±0.04 | 2.82±0.30 | 5.430±0.86 | 55.56±8.47 |
| Embodiment 1 | 5.19±0.12 | 4.84±0.21 | 22.58±0.84 | 84.02±4.65 |
| Embodiment 2 | 6.29±0.02 | 5.43±0.50 | 25.16±2.78 | 87.04±4.00 |
| Embodiment 3 | 5.97±0.13 | 5.42±0.25 | 24.62±3.92 | 89.24±5.55 |
| Embodiment 4 | 6.05±0.16 | 5.85±0.12 | 27.66±2.95 | 85.36±5.00 |
As can be seen from the table, stichopus japonicus composite immune reinforcing agent of the present invention is added in stichopus japonicus young children's ginseng feed, the specific growth rate of the young children's ginseng of stichopus japonicus, rate of body weight gain and AKP, ACP, LSZ and SOD enzyme activity can be significantly improved, a small amount of stichopus japonicus young children ginseng composite immune reinforcing agent just can significantly improve growth and the immunity of young children's ginseng, is feasible as the long-term immunopotentiator efficiently of stichopus japonicus young children ginseng.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that the common engineers and technicians in this area make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determine.
Claims (4)
1. a composite immune reinforcing agent for the young children's ginseng of stichopus japonicus, is characterized in that: be made up of the material of following parts by weight:
Astragalus polyose: 15-20;
Codonopsis pilosula polysaccharide: 10-15;
Radix Isatidis: 10-20;
Herba Andrographitis: 5-10;
Honeysuckle: 5-10;
Vitamin C: 0.5-5;
Vitamin E: 1-6;
Secondary powder: 20-40;
Described material is pulverized through micronizer, mixes after quantitative according to proportioning after 100 mesh sieves.
2. composite immune reinforcing agent according to claim 1, is characterized in that: be made up of the material of following parts by weight:
Astragalus polyose: 17;
Codonopsis pilosula polysaccharide: 15;
Radix Isatidis: 13;
Herba Andrographitis: 8;
Honeysuckle: 7;
Vitamin C: 3.5;
Vitamin E: 2.5;
Secondary powder: 33.
3. the application of the composite immune reinforcing agent described in claim 1 or 2 in stichopus japonicus young children's ginseng feed.
4. application according to claim 3, is characterized in that: the ratio being 0.2-0.5% according to mass ratio in feed adds described composite immune reinforcing agent.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991026A (en) * | 2010-11-24 | 2011-03-30 | 广州海因特生物技术有限公司 | Complex immunity reinforcing agent for improving stress resistance of black carp and preparation method thereof |
| CN102524599A (en) * | 2012-02-12 | 2012-07-04 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
| CN102550881A (en) * | 2012-02-12 | 2012-07-11 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991026A (en) * | 2010-11-24 | 2011-03-30 | 广州海因特生物技术有限公司 | Complex immunity reinforcing agent for improving stress resistance of black carp and preparation method thereof |
| CN102524599A (en) * | 2012-02-12 | 2012-07-04 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
| CN102550881A (en) * | 2012-02-12 | 2012-07-11 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
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Address after: 264000 Chaoshui Yaqian Village, Yantai Economic and Technological Development Zone, Shandong Province Patentee after: Shandong Anyuan Seed Technology Co.,Ltd. Address before: Penglai City, the town of Shandong village Nga 264000 city of Yantai Province Patentee before: SHANDONG ANYUAN AQUACULTURE Co.,Ltd. |
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Effective date of registration: 20220705 Address after: 264006 Yaqian village, Chaoshui, Yantai Economic and Technological Development Zone, Shandong Province Patentee after: Yantai peng'anyuan Marine Food Co.,Ltd. Address before: 264000 Chaoshui Yaqian Village, Yantai Economic and Technological Development Zone, Shandong Province Patentee before: Shandong Anyuan Seed Technology Co.,Ltd. |
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