CN103652381A - Composite immunopotentiator for young stichopus japonicus - Google Patents
Composite immunopotentiator for young stichopus japonicus Download PDFInfo
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- CN103652381A CN103652381A CN201210328024.0A CN201210328024A CN103652381A CN 103652381 A CN103652381 A CN 103652381A CN 201210328024 A CN201210328024 A CN 201210328024A CN 103652381 A CN103652381 A CN 103652381A
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Abstract
The invention relates to composite immunopotentiator for young stichopus japonicus and a preparation method thereof. The composite immunopotentiator comprises the following ingredients in parts by weight: 15 to 20 parts of astragalus polysaccharide, 10 to 15 parts of polysaccharides from radix codonopsis, 10 to 20 parts of isatis root, 5 to 10 parts of andrographis paniculata, 5 to 10 parts of double blossom, 0.5 to 5 parts of vitamin C, 1 to 6 parts of vitamin E and 20 to 40 parts of wheat middling; the ingredients are crushed through a super-micro crushing machine and uniformly mixed after being quantized according to the ratio by passing through a 100-mesh sieve; the composite immunopotentiator is added into the feed according to a weight ratio of 0.2 to 0.5 percent. By adopting the composite immunopotentiator, the non-specificity immunity of the young stichopus japonicus can be obviously improved, and the performance of the organism can be enhanced.
Description
Technical field
The present invention relates to a kind of feed addictive, particularly composite immune reinforcing agent of the young children's ginseng of a kind of stichopus japonicus use and preparation method thereof.
Background technology
Sea cucumber has higher edible medical value and economic worth, the precious marine product with special nourishing and nutrition and health care multi-efficiency of extensively being admitted.Along with the increase of people to stichopus japonicus demand, adopt and catch intensity and increase gradually, cause China's Holothurian Resources amount sharply to decline.Meanwhile, sea cucumber is propagated the continuous expansion of scale artificially, and sea cucumber germplasm degradation phenomena is on the rise, and the farming disease harms series of problems such as be on the rise, is seriously restricting the development of sea cucumber industry.
Immunopotentiator refers to by different modes to have and promote or bring out host to produce defense reaction, reaches the material that strengthens immunity of organisms.Main Function, in the nospecific immunity of aquatic products invertebrate, activates the immune defense system of body, thereby reach, strengthens the object that immunity improves its resistance against diseases.Immunopotentiator has safe ready, and noresidue and sphere of action be feature widely, aspect resistance disease and raising immunity of organisms, is obviously being better than vaccine and antibiotic.In current aquatic animal disease prevention and cure, antibiotic still plays a part main, but considers its resistance to the action of a drug and residue problem, and antibiotic is eliminated by people day by day, instead will be immunopotentiator and vaccine etc., vaccine is owing to there being specificity, therefore, and in disease preventing and treating, the broad spectrum activity that effect is not so good as immunopotentiator is good, using method and level of processing at vaccine are in addition limited, and therefore, immunopotentiator is subject to people's absolute favor at present.Aquaculture take " anti-overweight control " be principle, and the effect of immunopotentiator exactly focuses on anti-going up, and its also highly significant of curative effect aspect controlling.In addition, immunopotentiator is used jointly as adjuvant and vaccine, more can strengthen the curative effect of vaccine, and therefore, its development field is further widened.Along with the using method to vaccine reinforcing agent, dosage and time limit and further deep to the research of aquatic livestock immunologic mechanism, immunopotentiator will become control aquatic animal disease, improves very effectively approach of immunity.At present, by multi-source, multipath mode develops low toxicity, and efficient, quick-acting, Novel immune reinforcing agent has become the main trend of development longer.
Summary of the invention
Technical problem solved by the invention is to provide the young children's ginseng of a kind of stichopus japonicus composite immune reinforcing agent and preparation method thereof, it is added in feed and feeds and raise young children's ginseng, can improve the anti-stress ability of young children's ginseng, the damage that minimizing stress bring, the immunocompetence that strengthens body, improves its survival rate.
Improve a composite immune reinforcing agent for the young children ginseng anti-stress ability of stichopus japonicus, by the material of following parts by weight, formed:
Astragalus polyose: 15-20;
Codonopsis pilosula polysaccharide: 10-15;
Radix Isatidis: 10-20;
Herba Andrographitis: 5-10;
Honeysuckle (claiming again honeysuckle): 5-10;
Vitamin C: 0.5-5;
Vitamin E: 1-6;
Inferior powder: 20-40.
Described astragalus polyose is that the dry root of legume astragalus mongolicus or Astragalus membranacus is through the water-soluble heteroglycan extracting, concentrated, purifying forms.Faint yellow, powder is fine and smooth, evenly free from admixture.Astragalus polyose (Polysacchrides) content >=50% wherein.
Described Codonopsis pilosula polysaccharide is the Powdered polysaccharide compound that the dry root of Campanulaceae Radix Codonopsis, element flower Radix Codonopsis or radix codonpsis tangshen obtains after water extract-alcohol precipitation, wherein Codonopsis pilosula polysaccharide content >=30%.
Described powder is the part between flour and wheat bran, and flour mill can buy.
Composite immune reinforcing agent of the present invention, it is preferably comprised of the material of following parts by weight:
Astragalus polyose: 17;
Codonopsis pilosula polysaccharide: 15;
Radix Isatidis: 13;
Herba Andrographitis: 8;
Honeysuckle: 7;
Vitamin C: 3.5;
Vitamin E: 2.5;
Inferior powder: 33.
The preparation method of above-mentioned composite immune reinforcing agent, described material is pulverized through micronizer, after quantitative according to proportioning after 100 mesh sieves, mixes.
Above-mentioned composite immune reinforcing agent, is added into it in feed and feeds and raise young children's ginseng, can improve the anti-stress ability of young children's ginseng, and the ratio that is 0.2-0.5% according to mass ratio in feed is added described immunopotentiator.
Composite immune reinforcing agent provided by the invention, can obviously improve the non-specific immunity that the young children of stichopus japonicus joins, and strengthens the performance of body.
The specific embodiment
For further illustrating the present invention, illustrate with the following Examples:
The astragalus polyose adopting in following examples is purchased from Shaanxi Yong Yuan Bioisystech Co., Ltd, production code member: YYS-015.Product specification: astragalus polyose content 50%(mass concentration); 5:1(concentrates volume ratio).
Codonopsis pilosula polysaccharide is purchased from Shaanxi Yong Yuan Bioisystech Co., Ltd, production code member: YYR-040.Product specification: Codonopsis pilosula polysaccharide content 30%(mass concentration); 10:1(concentrates volume ratio).
Embodiment 1
Take astragalus polyose 17kg, Codonopsis pilosula polysaccharide 15kg, Radix Isatidis 13kg, Herba Andrographitis 8kg, honeysuckle 7kg, vitamin C 3.5kg, vitamin E 2.5kg, inferior powder 33kg, through micronizer, pulverize, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then according to 0.2% ratio, be added in the feed of stichopus japonicus.
Embodiment 2
Take astragalus polyose 15kg, Codonopsis pilosula polysaccharide 15kg, Radix Isatidis 20kg, Herba Andrographitis 5kg, honeysuckle 10kg, vitamin C 2kg, vitamin E 5kg, inferior powder 33kg, through micronizer, pulverize, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then according to 0.3% ratio, be added in the feed of stichopus japonicus.
Embodiment 3
Take astragalus polyose 20kg, Codonopsis pilosula polysaccharide 10kg, Radix Isatidis 15kg, Herba Andrographitis 10kg, honeysuckle 5kg, vitamin C 5kg, vitamin E 1kg, inferior powder 34kg, through micronizer, pulverize, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then according to 0.4% ratio, be added in the feed of stichopus japonicus.
Embodiment 4
Take astragalus polyose 19kg, Codonopsis pilosula polysaccharide 13kg, Radix Isatidis 17kg, Herba Andrographitis 6kg, honeysuckle 9kg, vitamin C 1kg, vitamin E 3kg, inferior powder 32kg, through micronizer, pulverize, after 100 eye mesh screens, mix, make composite immune reinforcing agent, then according to 0.5% ratio, be added in the feed of stichopus japonicus.
Test example:
One, the raising of stichopus japonicus and management
Select the same a collection of stichopus japonicus of hatching then as ginseng for experiment, initial body weight is 3.90 ± 0.84g.Experiment arranges 5 groups, is respectively control group, embodiment 1, embodiment 2, embodiment 3, embodiment 4.Wherein control group is thrown something and fed and is not added the apostichopus japonicus Selenka feed of immunopotentiator, and other embodiment groups have been added respectively the immunopotentiator of 0.2-0.5% on the basis of control group, and it forms and adding proportion is carried out according to embodiment 1-4.Every group 3 parallel, and each parallel 30 stichopus japonicus, throws something and feeds 1 every day, and Feeding time is 17:00, is satiated with food and throws something and feeds, and next day, 8:00 absorbed residual bait and ight soil.Continuous charge between feeding period, water temperature is controlled at 15 ± 0.5 ℃, salinity 32, PH7.8-8.2, dissolved oxygen is not less than 5mg/L.
Two, the collection of stichopus japonicus sample, analysis and calculating
Respectively within after throwing something and feeding the 10th, 20,30,40 and 50 days, measuring the various immuno-enzymatic activity of stichopus japonicus and growing state.Calculate the specific growth rate of stichopus japonicus.Computing formula is as follows:
Survival rate (%)=100 * (Nt-No)/No
Rate of body weight gain (WG, %)=100 * (Wt-Wo)/Wo
Specific growth rate (SGR, %)=100 * (ln Wt-ln Wo)/t
Wherein, No is respectively initial number of each parallel stichopus japonicus and an eventually last number with Nt, and Wt and Wo are respectively initial body weight and whole opisthosoma weight, and t is experiment number of days.
Get coelomic fluid, whole process is carried out on ice chest, prevents enzyme deactivation.Coelomic fluid is packed in 1.5mL centrifuge tube, be temporarily kept in 4 ℃ of refrigerators.Centrifugal, 3000r/min, 4 ℃ of centrifugal 10min, get supernatant ,-80 ℃ of preservations.With ELIASA, measure its AKP of absorbance measurement, ACP, LSZ and SOD enzyme activity.
Adopt SPSS 11.5for Windows to carry out one-way analysis of variance to the data obtained.
Three, experimental result is in Table 1 and table 2:
Table 1 is thrown something and fed, and compound immunopotentiator for apostichopus japonicus is grown on stichopus japonicus and the impact of survival rate
| Group | Survival rate % | Rate of body weight gain % | Specific growth rate % |
| Control group (A) | 97.12±0.83 | 2.56±0.78 | 0.02±0.01 |
| Embodiment 1 | 95.77±1.01 | 15.58±2.57 | 0.15±0.03 |
| Embodiment 2 | 98.85±0.86 | 14.42±1.84 | 0.10±0.05 |
| Embodiment 3 | 97.43±0.54 | 13.82±1.64 | 0.12±0.04 |
| Embodiment 4 | 98.50±0.47 | 15.21±2.75 | 0.14±0.02 |
Table 2 impact of compound immunopotentiator for apostichopus japonicus on stichopus japonicus immuno-enzymatic vigor of throwing something and feeding
| Group | AKP enzyme is lived | ACP enzyme is lived | LSZ enzyme is lived | SOD enzyme is lived |
| Control group (A) | 2.98±0.04 | 2.82±0.30 | 5.430±0.86 | 55.56±8.47 |
| Embodiment 1 | 5.19±0.12 | 4.84±0.21 | 22.58±0.84 | 84.02±4.65 |
| Embodiment 2 | 6.29±0.02 | 5.43±0.50 | 25.16±2.78 | 87.04±4.00 |
| Embodiment 3 | 5.97±0.13 | 5.42±0.25 | 24.62±3.92 | 89.24±5.55 |
| Embodiment 4 | 6.05±0.16 | 5.85±0.12 | 27.66±2.95 | 85.36±5.00 |
As can be seen from the table, in the young children's ginseng of stichopus japonicus feed, add stichopus japonicus composite immune reinforcing agent of the present invention, can significantly improve specific growth rate, rate of body weight gain and AKP, ACP, LSZ and the SOD enzyme activity of the young children's ginseng of stichopus japonicus, the young children's ginseng of a small amount of stichopus japonicus composite immune reinforcing agent just can significantly improve growth and the immunity of young children's ginseng, as the long-term immunopotentiator efficiently of the young children's ginseng of stichopus japonicus, is feasible.
Above-described embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; various distortion and improvement that the common engineers and technicians in this area make technical scheme of the present invention, all should fall in the definite protection domain of claims of the present invention.
Claims (5)
1. the young children of stichopus japonicus joins a composite immune reinforcing agent for use, it is characterized in that: the material by following parts by weight forms:
Astragalus polyose: 15-20;
Codonopsis pilosula polysaccharide: 10-15;
Radix Isatidis: 10-20;
Herba Andrographitis: 5-10;
Honeysuckle: 5-10;
Vitamin C: 0.5-5;
Vitamin E: 1-6;
Inferior powder: 20-40.
2. composite immune reinforcing agent according to claim 1, is characterized in that: the material by following parts by weight forms:
Astragalus polyose: 17;
Codonopsis pilosula polysaccharide: 15;
Radix Isatidis: 13;
Herba Andrographitis: 8;
Honeysuckle: 7;
Vitamin C: 3.5;
Vitamin E: 2.5;
Inferior powder: 33.
3. the preparation method of the composite immune reinforcing agent described in claim 1 or 2, is characterized in that: described material is pulverized through micronizer, after quantitative according to proportioning after 100 mesh sieves, mixes.
4. the application of the composite immune reinforcing agent described in claim 1 or 2 in the young children's ginseng of stichopus japonicus feed.
5. application according to claim 4, is characterized in that; The ratio that is 0.2-0.5% according to mass ratio in feed is added described composite immune reinforcing agent.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105146178A (en) * | 2015-09-06 | 2015-12-16 | 武汉中博绿亚生物科技有限公司 | Anti-stress preparation applied to culture of ornamental fish |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991026A (en) * | 2010-11-24 | 2011-03-30 | 广州海因特生物技术有限公司 | Complex immunity reinforcing agent for improving stress resistance of black carp and preparation method thereof |
| CN102524599A (en) * | 2012-02-12 | 2012-07-04 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
| CN102550881A (en) * | 2012-02-12 | 2012-07-11 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101991026A (en) * | 2010-11-24 | 2011-03-30 | 广州海因特生物技术有限公司 | Complex immunity reinforcing agent for improving stress resistance of black carp and preparation method thereof |
| CN102524599A (en) * | 2012-02-12 | 2012-07-04 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
| CN102550881A (en) * | 2012-02-12 | 2012-07-11 | 山东东方海洋科技股份有限公司 | Sea cucumber feed and production method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105146178A (en) * | 2015-09-06 | 2015-12-16 | 武汉中博绿亚生物科技有限公司 | Anti-stress preparation applied to culture of ornamental fish |
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Address after: 264000 Chaoshui Yaqian Village, Yantai Economic and Technological Development Zone, Shandong Province Patentee after: Shandong Anyuan Seed Technology Co.,Ltd. Address before: Penglai City, the town of Shandong village Nga 264000 city of Yantai Province Patentee before: SHANDONG ANYUAN AQUACULTURE Co.,Ltd. |
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Effective date of registration: 20220705 Address after: 264006 Yaqian village, Chaoshui, Yantai Economic and Technological Development Zone, Shandong Province Patentee after: Yantai peng'anyuan Marine Food Co.,Ltd. Address before: 264000 Chaoshui Yaqian Village, Yantai Economic and Technological Development Zone, Shandong Province Patentee before: Shandong Anyuan Seed Technology Co.,Ltd. |
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