CN103651772A - Sterilization composition - Google Patents
Sterilization composition Download PDFInfo
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- CN103651772A CN103651772A CN201310701433.5A CN201310701433A CN103651772A CN 103651772 A CN103651772 A CN 103651772A CN 201310701433 A CN201310701433 A CN 201310701433A CN 103651772 A CN103651772 A CN 103651772A
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- jiean
- peptide
- imazalil
- fruit
- aqueous emulsion
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Abstract
The invention belongs to the technical field of pesticides, and relates to sterilization composition. The sterilization composition is characterized in that active ingredients comprise Jiean-peptide and imazalil. The composition can be prepared into emulsion in water and used for orange preservation, and the preservation effect is obvious.
Description
Technical field
The invention belongs to technical field of pesticide, contain specifically the composite citrus preservative with synergistic effect of Jiean-peptide and imazalil.
Background technology
Jiean-peptide (jiean-peptide, be called for short JAA) be a kind of antifungal activity polypeptide being produced by bacillus, there is broad-spectrum antifungal activity, and good stability, antibacterial lasting, these product can pass through Antifungi spore germination, mycelial growth, thus the effect of performance antifungal growth.These product all have stronger inhibition growth and prevention effect to pathogens such as verticillium dahliae, cotton-wilt fusarium, fusarium graminearum, rhizoctonia cerealis, powdery mildew, eliminating vegetable botrytis and downy mildew, muskmelon phytophthora root rot bacterium.
Imazalil (Imazalil) is and is IMAZALIL, is systemic fungicide.Low to person poultry toxicity, be good fruit and vegetable preserving antistaling agent, to oranges and tangerines, busy fruit, banana, apple, melon particularly effective, the penicillium of resisting carbendazim, probenazole is had to special efficacy.Also can be used for preventing and treating cereal crops disease.
Oranges and tangerines are one of main fruit trees of southern china cultivation.Its fruit color, smell and taste are held concurrently excellent, and fruit juice is abundant, and local flavor is graceful, and nutritive value is high.Citrusfruit much more general in October with after ripening, because its ontogeny time is long, during maturation, environment temperature is lower, fruit metaboilic level is also lower, therefore from systematic growth and ontogeny, oranges and tangerines are the more long keeping fruit of a class.But after orange storing 1 month, the impact with storage environment temperature, humidity, gas componant of infecting due to microorganism, be prone to the phenomenons such as rotten, oedema, outer skin, had a strong impact on the commodity value of fruit, various storage diseases particularly, as brought larger economic loss often to numerous orchard workers by fungus-caused penicilliosis, green mould, black rot, blossom-end rot, anthracnose, sour rot etc.
Summary of the invention
The object of this invention is to provide a kind of bactericidal composition with synergistic effect and safety and environmental protection, realize the object that improves drug effect, reduces pollution, reduces costs.Be specifically related to the composite citrus preservative composition of Jiean-peptide and imazalil.
Utilize above-mentioned bactericidal composition, be equipped with the known auxiliary agent of those skilled in that art, with the known method of those skilled in that art, make aqueous emulsion of the present invention.
In above-mentioned bactericidal composition, the proportion of Jiean-peptide and imazalil is 1:50-50:1.
Above-mentioned bactericidal composition has following characteristics: 1. two kinds of compositions are composite, have synergistic effect; 2. bactericidal action is strong, is difficult for developing immunity to drugs; 3. formulation is advanced, environmental protection; 4. twenty percent divide between without cross resistance.
The specific embodiment
For determining Jiean-peptide and two kinds of composite synergistic effects of active ingredient of imazalil, first applicant uses these two kinds of compositions to carry out indoor biometrics test, and test method and result are as follows:
First analyzed after the effect feature of two kinds of active ingredients, we are decided to be Penicillium notatum by the raw object of surveying, and this mould can make pericarp deliquescing rot, and rapidly, at scab, place gently presses in expansion, and pericarp easily breaks.Disease portion first grows white hypha, changes soon green mould layer into, under hot and humid condition, from starting morbidity, to full fruit, rots only to need 1-2 week.
The test method of indoor biometrics: adopt growth rate method to measure the inhibitory action of medicament to the growth of Citrus fungal hyphae.On the basis of pilot study, from each medicament, germ mycelial growth inhibition rate is reached and within the scope of 10%-90%, designs 5 concentration, first the former medicine of 70% Jiean-peptide and the former medicine of 96% imazalil be take acetone as the liquid that solvent is made into series concentration standby, then by liquid in 1% ratio join melt and the PDA culture medium of cool to room temperature in, after fully shaking up, pour that in the culture dish (Φ 90mm) of sterilizing, to make band medicine dull and stereotyped into, every processing repeats for four times, to add the blank that is treated to of sterilized water; Card punch (Φ 5mm) picking Penicillium italicum and green mold germ cake with sterilizing, mycelia faces down aseptic inoculation in the central authorities with medicine flat board, be inverted in interior cultivation of constant incubator of 25 ℃, after cultivating 6d, adopt right-angled intersection method to measure colony diameter, calculate the EC50 of each medicament to pathogen, and calculate according to the following equation toxicity index, the virulence of more different medicaments and bacteriostasis.Former medicine used is tested in above-mentioned raw test and reagent is all bought from other producers.
The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:
The theoretical toxicity index TTI=∑ of mixture preparation (the toxicity index ATI of certain medicine * percentage of this pharmaceutically active ingredient in mixture).
It is synergistic effect that CTC is greater than at 120 o'clock, and being less than at 80 o'clock is antagonism, is addition in the time of between 80-120.
Table 1 Jiean-peptide, imazalil and the measurement result of different proportioning to Penicillium italicum co-toxicity coefficient thereof
Analyze above-mentioned data, can obviously find out when Jiean-peptide and imazalil are composite in varing proportions, formula is all greater than 120 to the co-toxicity coefficient of Penicillium italicum (CTC), Penicillium italicum prevention and control capability is had to the synergistic effect of different proportion, wherein Jiean-peptide and imazalil are being that proportioning five is visible to the synergy between proportioning seven between 1:1-10:1.
By indoor biometrics, test and determine Jiean-peptide and two kinds of active ingredients of imazalil are composite can good synergistic effect, can become the desirable medicament of control oranges and tangerines storage disease.Below by instantiation in order to further describe the present invention, but the present invention absolutely not only only limits to following these embodiment.In formula, percentage is all weight percentage, and the former medicine using and auxiliary agent are other producers and buy.
Formulation examples is as follows:
Example one, 25.5% Jiean-peptide imazalil aqueous emulsion
Example two, 22% Jiean-peptide imazalil aqueous emulsion
Example three, 18% Jiean-peptide imazalil aqueous emulsion
Example four, 8% Jiean-peptide imazalil aqueous emulsion
Example five, 12% Jiean-peptide imazalil aqueous emulsion
Example six, 33% Jiean-peptide imazalil aqueous emulsion
Example seven, 15.5% Jiean-peptide imazalil aqueous emulsion
Example eight, 25.5% Jiean-peptide imazalil aqueous emulsion
Example nine, 12% Jiean-peptide imazalil aqueous emulsion
Example ten, 9% Jiean-peptide imazalil aqueous emulsion
Select above example to prevent and treat the test of oranges and tangerines storage disease, determine the effect of formula control oranges and tangerines storage disease; Simultaneously by the Contrast on effect with Jiean-peptide and imazalil single dose, verify the synergistic effect after composite.Concrete test method data are as follows:
12% Jiean-peptide imazalil aqueous emulsion and other preparation are cooked the oranges and tangerines storage disease test of pesticide effectiveness.Test site is Ganzhou, and the time is on November 12nd, 2012, converts 1kg water, steeps fruit, the oranges and tangerines that every processing bubble 100kg has just plucked.Test each and process three repetitions, random district group is arranged, and blank is processed with clear water.The storage condition of all districts group is identical, in same storeroom, carries out.Within after medicine 60 days, carry out the investigation of decayed fruit amount.
Investigation method: record the decayed fruit amount of each processing, weigh, calculate fruit rate.
Table 212% Jiean-peptide imazalil aqueous emulsion and the fresh-keeping effect of different medicament to oranges and tangerines
From above-mentioned data, can find out, Jiean-peptide and imazalil complex preparation are steeping fruit latter 60 days, fresh-keeping effect is all over 81%, and Jiean-peptide single dose is 60.32% at latter 60 days fresh-keeping effects of bubble fruit, the fresh-keeping effect of imazalil single dose is 64.43%, the preventive effect of complex preparation is compared with single dose has utmost point significant difference, after visible two kinds of active ingredients are composite, has good preservation, has extended the storage time of oranges and tangerines.
Claims (3)
1. a bactericidal composition, is characterized in that: active component is comprised of Jiean-peptide and imazalil, and the weight ratio of described Jiean-peptide and imazalil is 1:50～50:1.
2. bactericidal composition according to claim 1, is characterized in that: by active ingredient and auxiliary material, make aqueous emulsion.
3. according to claim 1 or bactericidal composition claimed in claim 2, it is characterized in that: fresh-keeping for oranges and tangerines.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701433.5A CN103651772B (en) | 2013-12-18 | 2013-12-18 | Sterilization composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310701433.5A CN103651772B (en) | 2013-12-18 | 2013-12-18 | Sterilization composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103651772A true CN103651772A (en) | 2014-03-26 |
| CN103651772B CN103651772B (en) | 2014-10-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310701433.5A Active CN103651772B (en) | 2013-12-18 | 2013-12-18 | Sterilization composition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105477A (en) * | 2018-08-28 | 2019-01-01 | 靖西海越农业有限公司 | A kind of preservation method of citrus composite preservative and citrus |
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| US5215747A (en) * | 1992-02-07 | 1993-06-01 | Uniroyal Chemical Company, Inc. | Composition and method for protecting plants from phytopathogenic fungi |
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| CN101156627A (en) * | 2007-09-21 | 2008-04-09 | 华南农业大学 | Citrus corrosion protection antistaling agent with tea saponin |
| CN101416628A (en) * | 2008-11-06 | 2009-04-29 | 张少武 | Agricultural bactericidal agent containing imazalil |
| CN101416657A (en) * | 2007-10-26 | 2009-04-29 | 中国科学院成都生物研究所 | Fruit anti-staling agent |
| WO2013178648A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
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| US5215747A (en) * | 1992-02-07 | 1993-06-01 | Uniroyal Chemical Company, Inc. | Composition and method for protecting plants from phytopathogenic fungi |
| CN1699585A (en) * | 2004-05-20 | 2005-11-23 | 中国科学院成都生物研究所 | Process for preparing biological antibacterial agent |
| CN101156627A (en) * | 2007-09-21 | 2008-04-09 | 华南农业大学 | Citrus corrosion protection antistaling agent with tea saponin |
| CN101416657A (en) * | 2007-10-26 | 2009-04-29 | 中国科学院成都生物研究所 | Fruit anti-staling agent |
| CN101416628A (en) * | 2008-11-06 | 2009-04-29 | 张少武 | Agricultural bactericidal agent containing imazalil |
| WO2013178648A1 (en) * | 2012-05-30 | 2013-12-05 | Bayer Cropscience Ag | Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis |
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| XIAOYONG ZHANG等: "Response surface methodology used for statistical optimization of jie-an peptide production by Bacillus subtilis", 《ELECTRONIC JOURNAL OF BIOTECHNOLOGY》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109105477A (en) * | 2018-08-28 | 2019-01-01 | 靖西海越农业有限公司 | A kind of preservation method of citrus composite preservative and citrus |
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| CN103651772B (en) | 2014-10-29 |
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Inventor after: Liu Xiaojiao Inventor after: Yang Huiying Inventor after: Wu Di Inventor after: Wu Yong Inventor after: Ma Gaoyue Inventor after: Cai Chaoyang Inventor before: Liu Xiaojiao Inventor before: Yang Huiying Inventor before: Wu Yong Inventor before: Ma Songyue Inventor before: Cai Chaoyang |
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Free format text: CORRECT: INVENTOR; FROM: LIU XIAOJIAO YANG HUIYING WU YONG MA SONGYUE CAI ZHAOYANG TO: LIU XIAOJIAO YANG HUIYING WU DI WU YONG MA GAOYUE CAI ZHAOYANG |
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Effective date of registration: 20171218 Address after: 063600 Leting County Economic Development Zone, Tangshan City, Hebei Patentee after: Yanhua Yongle (Leting) Biological Technology Co. Ltd. Address before: 102488 No. 8, Liangxiang, Beijing, Fangshan District Patentee before: Beijing Yanhua Yongle Biotechnology Co., Ltd. |
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