CN103636596A - Bio-membrane protective solution as well as preparation and application thereof - Google Patents
Bio-membrane protective solution as well as preparation and application thereof Download PDFInfo
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- CN103636596A CN103636596A CN201310617308.6A CN201310617308A CN103636596A CN 103636596 A CN103636596 A CN 103636596A CN 201310617308 A CN201310617308 A CN 201310617308A CN 103636596 A CN103636596 A CN 103636596A
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Abstract
The invention relates to a bio-membrane protective solution as well as preparation and application thereof. The protective solution is prepared from the following three components in percentage by volume: 1 percent of 14 percent peroxyacetic acid, 14 percent of 95 percent ethanol, and 85 percent of sterilized physiological saline. The protective solution is mainly applied to bio-membrane storage, and a bio-membrane to be stored is sterilized by using the protective solution and is put in a seal bag filled with the protective solution for storage. The protective solution has the effects of diminishing inflammation, inhibiting bacteria or sterilizing, disinfecting a wound to keep the wound in a sterile environment, can be used for washing and protecting a bio-membrane, and can be used for expanding and contracting blood vessels to achieve the effects of reducing wound effusion, reducing soft tissue edema and relieving pain, so that an auxiliary treatment effect is played on the wound by using the protective solution to protect a bio-membrane; the preparation method of the protective solution is simple and convenient, raw materials are low in cost and easily available, the use is convenient, and the effect is good.
Description
Technical field
The present invention relates to a kind of protection liquid in field of medicaments, be specifically related to a kind of biomembrane protection liquid and preparation and application thereof.
Background technology
Biomembrane is defined as the phospholipid bilayer that is inlaid with protein and sugar class (general designation glycoprotein) biologically, playing division is also the significant points relevant with communication in many Conversion of Energies and cell with separating cell and crganelle effect biomembrane, meanwhile, on biomembrane, also has a large amount of enzyme binding sites.Cell, organelle and its environment connect the general name of all membrane structures on boundary; In biology, except some virus, all there is biomembrane.
And biomembrane of the present invention mainly refers to human or animal's placenta, amnion, chorion and artificial skin etc.Amnion and chorion are two chief components of placenta, amnion is at the innermost layer of placenta, and film body is smooth, without blood vessel, nerve and lymph, have certain elasticity, it contains and abundant has multipotency, can not repel anti-multipotential stem cell by induction of immunity after transplanting.Amniotic cell of human placenta also can be expressed nerve cell specific mark, secretory nerve trophic factors, and for nervous system disease, in addition people's placenta amnion can also be as the basilar memebrane of ophthalmologic operation; And chorion is placenta main part, adjacent with amnion, on chorion, contain equally the abundant stem cell with many differentiation potentials, in the injuries of tissues and organs reparation and transplanting that can be applicable to cause due to old and feeble and pathology.
These biomembranes medically have use widely; while there is the situations such as burn, scald, scratch such as human body skin; biomembrane has the effect of protection or wound repairing; these biomembranes just need to clean, preserve their medium so, and these media are exactly the biomembrane protection liquid that the present invention mentions.Utopian protection liquid, except needs possess protection biomembranous biologically active do not suffer a loss, also needs to preserve for a long time biomembrane, to such an extent as to can satisfy the demand at any time.
Summary of the invention
The object of this invention is to provide a kind of biomembrane protection liquid and preparation and application thereof, this protection liquid cost of material is low, and preparation is simple, easy to use, effective; Its apart from killing, antibacterial outside, collapsible hemangiectasis again simultaneously, reaches and reduces the surface of a wound and ooze out, and reduces soft tissue oedema, and the effect of pain relieving; It is mainly used in biomembranous preservation.
The object of the invention is to be achieved through the following technical solutions:
A biomembrane protection liquid, described protection liquid is formulated by Peracetic acid, ethanol and physiological saline, the volume ratio of described three kinds of materials is physiological saline 82～88%, ethanol 11～17% and Peracetic acid 0.8～1.2%.
Further, the volume ratio that forms three kinds of materials of described protection liquid is physiological saline 85%, ethanol 14% and 1 Peracetic acid 1%.
Further, described Peracetic acid is the Peracetic acid of purity 14%.
Further, the ethanol that described ethanol is 95%.
Further, described physiological saline is sterile saline.
A kind of preparation method of described biomembrane protection liquid comprises the following steps:
1. physiological saline is carried out to sterilizing, make sterile saline;
2. 95% ethanol is added to the sterile saline of step described in 1., stirs, obtain solution A;
3. 14% Peracetic acid is added in the solution A of step described in 2. again, mixes, make described protection liquid.
The application of protection liquid described in preparation in biomembrane is preserved, the placenta that described biomembrane is human or animal, amnion, chorion and artificial skin; Described store method is: the biomembrane that needs are preserved carries out sterilization treatment, is positioned in the sealed bag that described protection liquid is housed, and carries out low temperature preservation; Described protection liquid has the effect of anti-inflammatory, antibacterial or sterilization, is used for cleaning, protection biomembrane.
The invention provides a kind of biomembrane protection liquid and preparation and application thereof, it mainly has following some beneficial effect:
1. protection liquid of the present invention belongs to the chemical reagent without any toxicity, side effect; There is sterilization, sterilization, antibacterial effect;
2. this protection liquid is mainly used to clean, preserve biomembrane; With it, preserve biomembrane, this protection liquid also has for surface of a wound sterilization, makes the surface of a wound keep the effect of gnotobasis;
3. this protection liquid collapsible hemangiectasis again simultaneously, reaches and reduces the surface of a wound and ooze out, and reduces soft tissue oedema, and the effect of pain relieving, therefore preserve biomembrane with this protection liquid, it also has auxiliary result for the treatment of to the surface of a wound;
4. this protection liquid can be preserved for a long time, never degenerates;
5. biomembrane is preserved in this protection liquid, makes biomembrane keep for a long time physiologically active, and can save backup for a long time, and reserved sample observing is 20 months, is not any change, and takes sample to do bacterial culture without bacterial growth;
6. the compound method of this protection liquid is simple and convenient, and cost of material is low, is easy to get, easy to use, effective.
Embodiment
A kind of biomembrane protection liquid described in the embodiment of the present invention, this protection liquid is formulated by Peracetic acid, ethanol and physiological saline, the volume ratio of these three kinds of materials is physiological saline 82～88%, ethanol 11～17% and Peracetic acid 0.8～1.2%; The volume ratio that forms three kinds of materials of this protection liquid is preferably physiological saline 85%, ethanol 14% and 1 Peracetic acid 1%; This Peracetic acid is preferably the Peracetic acid of purity 14%, and ethanol is preferably 95% ethanol, and physiological saline is preferably sterile saline.
A kind of preparation method of this biomembrane protection liquid comprises the following steps:
1. physiological saline is carried out to sterilizing, make sterile saline;
2. 95% ethanol is added to the sterile saline of step described in 1., stirs, obtain solution A;
3. 14% Peracetic acid is added in the solution A of step described in 2. again, mixes, make described protection liquid.
This protection liquid is mainly used in biomembranous preservation, the placenta that described biomembrane is human or animal, amnion, chorion and artificial skin; Described store method is: the biomembrane that needs are preserved carries out sterilization treatment, is positioned in the sealed bag that described protection liquid is housed, and carries out low temperature preservation; Described protection liquid has the effect of anti-inflammatory, antibacterial or sterilization, is used for cleaning, protection biomembrane.
Specific experiment case take below as example illustrates embodiment, should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1: the allergic experiment of biomembrane protection liquid
Biological wound-protecting film is the biological wound-protecting film being kept in protection liquid of the present invention; Small white mouse used is Kunming kind small white mouse, female, hero half and half, and pharmacy one factory's Animal House in Shenyang provides.
Get 20 of body weight 20～22g healthy mices, cut off the long-pending 2 * 2cm of back wool fermentation, local painting protection liquid of the present invention is appropriate, every day 1 time, for three days on end; Then paste biological wound-protecting film, after being coated with for the first time protection liquid the 14th day, wound-protecting film self falling; Repeat again aforesaid operations, observe local situation.Result has no and is coated with protection liquid and pastes wound-protecting film place and have the phenomenons such as any rubescent, fash, oedema, foaming, and animal is also without any general reaction; Illustrate that this protection liquid and biological wound-protecting film are without local irritation allergy.
Embodiment 2: biomembrane protection liquid bacteriostatic experiment
In Vitro Bacteriostasis experiment adopts three methods: i.e. nutrient agar plate experiment (flat band method), nutrient agar plate experiment increasing bacterium method of scoring and meat soup are cultivated experimental method, adopt six kinds of standard pathogenic strains.Result shows: biomembrane protection liquid all has inhibitory action to Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus, staphylococcus albus, four folded coccuses and sarcine etc.
During concrete use, the partially shallow fresh surface of a wound wound of shallow II degree and dark II degree, after 1～8 hour, after covering, is substantially pasted a surface of a wound and can be healed with the biological wound-protecting film that is accompanied with protection liquid.And for the large fresh surface of a wound of area, after wound, in 1～2 hour, use, after covering, skin diffusate may be partly excessive along edge, about 2 hours, stops; Indivedual wound-protecting films may attach protection liquid too many after taking out from protection liquid, can scoop up a duck eye in the region of interest of wound-protecting film and emit protection liquid, are also substantially to paste a surface of a wound can heal.
The present invention is not limited to above-mentioned preferred forms, any modification relevant of the present invention or change that anyone does under enlightenment of the present invention, and every have identical with a application or akin technical scheme, within all dropping on protection scope of the present invention.
Claims (7)
1. a biomembrane protection liquid, is characterized in that: described protection liquid is formulated by Peracetic acid, ethanol and physiological saline, and the volume ratio of described three kinds of materials is physiological saline 82～88%, ethanol 11～17% and Peracetic acid 0.8～1.2%.
2. biomembrane protection liquid according to claim 1, is characterized in that: the volume ratio that forms three kinds of materials of described protection liquid is physiological saline 85%, ethanol 14% and 1 Peracetic acid 1%.
3. biomembrane protection liquid according to claim 1 and 2, is characterized in that: described Peracetic acid is the Peracetic acid of purity 14%.
4. biomembrane protection liquid according to claim 1 and 2, is characterized in that: the ethanol that described ethanol is 95%.
5. biomembrane protection liquid according to claim 1 and 2, is characterized in that: described physiological saline is sterile saline.
6. a preparation for biomembrane protection liquid according to claim 1 and 2, this preparation method comprises the following steps:
1. physiological saline is carried out to sterilizing, make sterile saline;
2. 95% ethanol is added to the sterile saline of step described in 1., stirs, obtain solution A;
3. 14% Peracetic acid is added in the solution A of step described in 2. again, mixes, make described protection liquid.
7. according to the prepared biomembrane of claim 6, protect the application of liquid in biomembrane is preserved, it is characterized in that: placenta, amnion, chorion and artificial skin that described biomembrane is human or animal; The biomembranous method of described preservation is: the biomembrane that needs are preserved carries out sterilization treatment, is positioned in the sealed bag that described protection liquid is housed, and carries out low temperature preservation; Described protection liquid has the effect of anti-inflammatory, antibacterial or sterilization, is used for cleaning, protection biomembrane.
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| CN201310617308.6A CN103636596B (en) | 2013-11-29 | 2013-11-29 | A kind of biomembrane protection liquid and preparation and application thereof |
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| CN201310617308.6A CN103636596B (en) | 2013-11-29 | 2013-11-29 | A kind of biomembrane protection liquid and preparation and application thereof |
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| CN103636596A true CN103636596A (en) | 2014-03-19 |
| CN103636596B CN103636596B (en) | 2016-02-24 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104208751A (en) * | 2014-08-19 | 2014-12-17 | 温州医科大学 | Preparation method for novel kidney acellularized biological scaffold |
| CN108812643A (en) * | 2018-07-18 | 2018-11-16 | 银丰生物工程集团有限公司 | Human placenia membrane tissue prepares cryopreservation methods and application |
| CN112535681A (en) * | 2019-09-23 | 2021-03-23 | 华南理工大学 | Arylsulfatase and glucuronidase inhibitor and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072086A (en) * | 1992-02-22 | 1993-05-19 | 郝振林 | The preparation method of cover biomembrane for wound of burn and scald |
| WO2007044047A1 (en) * | 2005-10-13 | 2007-04-19 | Alamo Scientific, Inc. | Apparatus and method for microbial and forensic sampling and manipulation |
| CN103392691A (en) * | 2013-08-14 | 2013-11-20 | 北京瑞健高科生物科技有限公司 | Container and method for processing, sterilizing and saving biological tissues |
-
2013
- 2013-11-29 CN CN201310617308.6A patent/CN103636596B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072086A (en) * | 1992-02-22 | 1993-05-19 | 郝振林 | The preparation method of cover biomembrane for wound of burn and scald |
| WO2007044047A1 (en) * | 2005-10-13 | 2007-04-19 | Alamo Scientific, Inc. | Apparatus and method for microbial and forensic sampling and manipulation |
| CN103392691A (en) * | 2013-08-14 | 2013-11-20 | 北京瑞健高科生物科技有限公司 | Container and method for processing, sterilizing and saving biological tissues |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104208751A (en) * | 2014-08-19 | 2014-12-17 | 温州医科大学 | Preparation method for novel kidney acellularized biological scaffold |
| CN108812643A (en) * | 2018-07-18 | 2018-11-16 | 银丰生物工程集团有限公司 | Human placenia membrane tissue prepares cryopreservation methods and application |
| CN112535681A (en) * | 2019-09-23 | 2021-03-23 | 华南理工大学 | Arylsulfatase and glucuronidase inhibitor and preparation method and application thereof |
| CN112535681B (en) * | 2019-09-23 | 2024-01-23 | 华南理工大学 | Arylsulfatase and glucuronidase inhibitor, and preparation method and application thereof |
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| CN103636596B (en) | 2016-02-24 |
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Effective date of registration: 20190219 Address after: 100013 Beijing Dongcheng District and Heping Li Seven District 26-1-228 Patentee after: Qu Kaiming Address before: Room 419, Block B, 18 Xihuan South Road, Beijing Economic and Technological Development Zone, 100176 Patentee before: DONGFANG KANGRUI (BEIJING) TECHNOLOGY DEVELOPMENT CO., LTD. |
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