CN103636596B - A kind of biomembrane protection liquid and preparation and application thereof - Google Patents
A kind of biomembrane protection liquid and preparation and application thereof Download PDFInfo
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- CN103636596B CN103636596B CN201310617308.6A CN201310617308A CN103636596B CN 103636596 B CN103636596 B CN 103636596B CN 201310617308 A CN201310617308 A CN 201310617308A CN 103636596 B CN103636596 B CN 103636596B
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Abstract
The present invention relates to a kind of biomembrane protection liquid and preparation and application thereof, described protection liquid is formulated by volume by following three kinds of materials, namely 14% Peracetic acid 1%, 95% ethanol 14% and sterile saline 85% formulated.This protection liquid is mainly used in biomembrane preservation, when specifically using, by needing the biomembrane preserved to carry out sterilization treatment, being positioned in the sealed bag that described protection liquid is housed, preserving.This protection liquid has anti-inflammatory, antibacterial or sterilization, is surface of a wound sterilization, makes the surface of a wound keep the effect of gnotobasis, can be used to cleaning, protection biomembrane; This protection liquid collapsible hemangiectasis again simultaneously, reaches and reduces the surface of a wound and ooze out, reduce soft tissue oedema, and the effect of pain relieving, therefore preserves biomembrane with this protection liquid, and it also has auxiliary result for the treatment of to the surface of a wound; And the compound method of this protection liquid is simple and convenient, cost of material is low, is easy to get, easy to use, effective.
Description
Technical field
The present invention relates to the one protection liquid in field of medicaments, be specifically related to a kind of biomembrane protection liquid and preparation and application thereof.
Background technology
Biomembrane is biologically being defined as the phospholipid bilayer being inlaid with protein and sugar class (general designation glycoprotein), playing division with separation cell and crganelle effect biomembrane is also the significant points relevant with intracellular communication with many Conversion of Energies, meanwhile, biomembrane also has a large amount of enzyme binding sites.Cell, organelle and its environment connect the general name of all membrane structures on boundary; In biology except some virus, all there is biomembrane.
And biomembrane of the present invention mainly refers to the placenta of human or animal, amnion, chorion and artificial skin etc.Amnion and chorion are two chief components of placenta, amnion is at the innermost layer of placenta, and film body is smooth, without blood vessel, nerve and lymph, there is certain elasticity, its contain abundant there is multipotency, transplant after can not the anti-multipotential stem cell of inducing immune-rejection.Amniotic cell of human placenta also can express nerve cell specific mark, secretory nerve trophic factors, and for nervous system disease, in addition Human plactnta amnion can also as the basilar memebrane of ophthalmologic operation; And chorion is placenta main part, adjacent with amnion, same containing the abundant stem cell with many differentiation potentials on chorion, can be applicable in injuries of tissues and organs reparation because old and feeble and pathology cause and transplanting.
These biomembranes medically have use widely; when such as there is the situations such as burn, scald, scratch in human body skin; biomembrane has the effect of protection or wound repairing; so these biomembranes just need the medium cleaning, preserve them, and these media are exactly the biomembrane protection liquid that the present invention mentions.Utopian protection liquid, except needs possess protection biomembranous biologically active do not suffer a loss, also needs to preserve biomembrane for a long time, to such an extent as to can satisfy the demand at any time.
Summary of the invention
The object of this invention is to provide a kind of biomembrane protection liquid and preparation and application thereof, this protection liquid cost of material is low, and preparation is simple, easy to use, effective; Its apart from killing, antibacterial outside, collapsible hemangiectasis again simultaneously, reaches and reduces the surface of a wound and ooze out, reduce soft tissue oedema, and the effect of pain relieving; It is mainly used in biomembranous preservation.
The object of the invention is to be achieved through the following technical solutions:
A kind of biomembrane protection liquid, described protection liquid is formed by Peracetic acid, ethanol and normal saline, and the volume ratio of described three kinds of materials is physiological saline 82 ~ 88%, ethanol 11 ~ 17% and Peracetic acid 0.8 ~ 1.2%.
Further, the volume ratio forming three kinds of materials of described protection liquid is physiological saline 85%, ethanol 14% and Peracetic acid 1%.
Further, described Peracetic acid is the Peracetic acid of purity 14%.
Further, described ethanol is the ethanol of 95%.
Further, described physiological saline is sterile saline.
A kind of preparation method of described biomembrane protection liquid comprises the following steps:
1. physiological saline is carried out sterilizing, obtained sterile saline;
2. the ethanol of 95% is added to step 1. described in sterile saline, stir, obtain solution A;
3. the Peracetic acid of 14% is added to again step 2. described in solution A in, mix, i.e. obtained described protection liquid.
The application of protection liquid described in preparation in biomembrane is preserved, described biomembrane is the placenta of human or animal, amnion, chorion and artificial skin; Described store method is: by needing the biomembrane preserved to carry out sterilization treatment, being positioned in the sealed bag that described protection liquid is housed, carrying out Cord blood; Described protection liquid has the effect of anti-inflammatory, antibacterial or sterilization, is used for cleaning, protection biomembrane.
The invention provides a kind of biomembrane protection liquid and preparation and application thereof, it mainly has some beneficial effect following:
1. protection liquid of the present invention belongs to the chemical reagent without any toxicity, side effect; There is sterilization, sterilization, antibacterial effect;
2. this protection liquid is mainly used to cleaning, preserves biomembrane; Preserve biomembrane with it, this protection liquid also has for surface of a wound sterilization, makes the surface of a wound keep the effect of gnotobasis;
3. this protection liquid collapsible hemangiectasis again simultaneously, reaches and reduces the surface of a wound and ooze out, reduce soft tissue oedema, and the effect of pain relieving, therefore preserves biomembrane with this protection liquid, and it also has auxiliary result for the treatment of to the surface of a wound;
4. this protection liquid can be preserved for a long time, never degenerates;
5. biomembrane is preserved in this protection liquid, makes biomembrane keep physiologically active for a long time, and can save backup for a long time, and reserved sample observing 20 months, is not any change, takes sample to do bacterial culture without bacterial growth;
6. the compound method of this protection liquid is simple and convenient, and cost of material is low, is easy to get, easy to use, effective.
Embodiment
A kind of biomembrane protection liquid described in the embodiment of the present invention, this protection liquid is formed by Peracetic acid, ethanol and normal saline, and the volume ratio of these three kinds of materials is physiological saline 82 ~ 88%, ethanol 11 ~ 17% and Peracetic acid 0.8 ~ 1.2%; The volume ratio forming three kinds of materials of this protection liquid is preferably physiological saline 85%, ethanol 14% and Peracetic acid 1%; This Peracetic acid is preferably the Peracetic acid of purity 14%, and ethanol is preferably the ethanol of 95%, and physiological saline is preferably sterile saline.
A kind of preparation method of this biomembrane protection liquid comprises the following steps:
1. physiological saline is carried out sterilizing, obtained sterile saline;
2. the ethanol of 95% is added to step 1. described in sterile saline, stir, obtain solution A;
3. the Peracetic acid of 14% is added to again step 2. described in solution A in, mix, i.e. obtained described protection liquid.
This protection liquid is mainly used in biomembranous preservation, and described biomembrane is the placenta of human or animal, amnion, chorion and artificial skin; Described store method is: by needing the biomembrane preserved to carry out sterilization treatment, being positioned in the sealed bag that described protection liquid is housed, carrying out Cord blood; Described protection liquid has the effect of anti-inflammatory, antibacterial or sterilization, is used for cleaning, protection biomembrane.
For specific experiment case, embodiment is described below, should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment 1: the allergic experiment of biomembrane protection liquid
Biological wound-protecting film is be kept at the biological wound-protecting film in protection liquid of the present invention; Small white mouse used is Kun Ming mice, and female, hero half and half, Shenyang pharmacy one factory Animal House provides.
Get body weight 20 ~ 22g healthy mice 20, cut off the long-pending 2 × 2cm of back wool fermentation, it is appropriate that local is coated with protection liquid of the present invention, every day 1 time, for three days on end; Then paste biological wound-protecting film, in first time be coated with protection liquid after the 14th day, wound-protecting film self falling; Repeat aforesaid operations again, observe the situation of local.Result has no and is coated with protection liquid and pastes wound-protecting film place and have the phenomenon such as any rubescent, fash, oedema, foaming, and animal is also without any general reaction; Illustrate that this protection liquid and biological wound-protecting film are without local irritation allergy.
Embodiment 2: biomembrane protection liquid bacteriostatic experiment
Antibacterial experiment in vitro adopts three methods: namely nutrient agar plate experiment (flat band method), the experiment of nutrient agar plate increase bacterium method of scoring and meat soup cultivation experimental method, adopt six kinds of standard pathogenic strains.Result shows: biomembrane protection liquid all has inhibitory action to Escherichia coli, Pseudomonas aeruginosa, staphylococcus aureus, staphylococcus albus, four folded coccus and sarcines etc.
During concrete use, shallow II degree and the partially shallow fresh surface of a wound wound of dark II degree be after 1 ~ 8 hour, after covering, substantially pastes a surface of a wound and can heal with the biological wound-protecting film being accompanied with protection liquid.And for the large fresh surface of a wound of area, use in 1 ~ 2 hour after wound, after covering skin diffusate may part excessive along edge, 2 hours stop; After indivedual wound-protecting film is taken out from protection liquid, incidentally may protect liquid too much, can scoop up a duck eye release protection liquid in the region of interest of wound-protecting film, be also that a basic subsides surface of a wound can heal.
The present invention is not limited to above-mentioned preferred forms, anyone for the present invention any modification done under enlightenment of the present invention or change, and every have identical with the application or akin technical scheme, all drops within protection scope of the present invention.
Claims (2)
1. a preparation method for biomembrane protection liquid, this preparation method comprises the following steps:
1. physiological saline is carried out sterilizing, obtained sterile saline;
2. the ethanol of 95% is added to step 1. described in sterile saline, stir, obtain solution A;
3. the Peracetic acid of 14% is added to again step 2. described in solution A in, mix, i.e. obtained described protection liquid;
In described protection liquid, the volume ratio of three kinds of materials is the Peracetic acid 1% of the ethanol 14% and 14% of sterile saline 85%, 95%.
2. the application of biomembrane protection liquid in biomembrane is preserved prepared by claim 1, is characterized in that: described biomembrane is the placenta of human or animal, amnion, chorion and artificial skin; The biomembranous method of described preservation is: by needing the biomembrane preserved to carry out sterilization treatment, being positioned in the sealed bag that described protection liquid is housed, carrying out Cord blood; Described protection liquid has the effect of anti-inflammatory, antibacterial or sterilization, is used for cleaning, protection biomembrane.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310617308.6A CN103636596B (en) | 2013-11-29 | 2013-11-29 | A kind of biomembrane protection liquid and preparation and application thereof |
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| CN201310617308.6A CN103636596B (en) | 2013-11-29 | 2013-11-29 | A kind of biomembrane protection liquid and preparation and application thereof |
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| CN103636596A CN103636596A (en) | 2014-03-19 |
| CN103636596B true CN103636596B (en) | 2016-02-24 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104208751A (en) * | 2014-08-19 | 2014-12-17 | 温州医科大学 | Preparation method for novel kidney acellularized biological scaffold |
| CN108812643B (en) * | 2018-07-18 | 2021-09-21 | 银丰生物工程集团有限公司 | Preparation and cryopreservation method and application of human placental chorionic villus tissue |
| CN112535681B (en) * | 2019-09-23 | 2024-01-23 | 华南理工大学 | Arylsulfatase and glucuronidase inhibitor, and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072086A (en) * | 1992-02-22 | 1993-05-19 | 郝振林 | The preparation method of cover biomembrane for wound of burn and scald |
| WO2007044047A1 (en) * | 2005-10-13 | 2007-04-19 | Alamo Scientific, Inc. | Apparatus and method for microbial and forensic sampling and manipulation |
| CN103392691A (en) * | 2013-08-14 | 2013-11-20 | 北京瑞健高科生物科技有限公司 | Container and method for processing, sterilizing and saving biological tissues |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072086A (en) * | 1992-02-22 | 1993-05-19 | 郝振林 | The preparation method of cover biomembrane for wound of burn and scald |
| WO2007044047A1 (en) * | 2005-10-13 | 2007-04-19 | Alamo Scientific, Inc. | Apparatus and method for microbial and forensic sampling and manipulation |
| CN103392691A (en) * | 2013-08-14 | 2013-11-20 | 北京瑞健高科生物科技有限公司 | Container and method for processing, sterilizing and saving biological tissues |
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Effective date of registration: 20190219 Address after: 100013 Beijing Dongcheng District and Heping Li Seven District 26-1-228 Patentee after: Qu Kaiming Address before: Room 419, Block B, 18 Xihuan South Road, Beijing Economic and Technological Development Zone, 100176 Patentee before: DONGFANG KANGRUI (BEIJING) TECHNOLOGY DEVELOPMENT CO., LTD. |
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