CN103609434B - High-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds - Google Patents
High-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds Download PDFInfo
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Abstract
The invention provides a high-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds. The high-yield crossbreeding method comprises the following steps: A, carrying out phenotypic screening on a GuiKe-2S large group, namely, planting a GuiKe-2S group of a single plant; B, detecting a specificity marker of a GuiKe-2S sterile locus; C, detecting a molecular marker of a GuiKe-2S fingerprint spectrum; D, carrying out low-temperature screening on a GuiKe-2S first-time manual climatic box; E, carrying out low-temperature screening on a GuiKe-2S second-time manual climatic box; and F, breeding a rice root in winter of Hainan, transplanting the GuiKe-2S the root of which is cut again to Sanya of Hainan for breeding in winter, and harvesting seeds when ripening to obtain the two-line super rice parent GuiKe-2S stock seeds. According to the high-yield crossbreeding method, the stock seeds are further bred to obtain high-purity parent seeds applied to seed production, which has a very important significance in developing two-line hybrid rice. Through tests, the purity of the obtained breeder seeds reaches above 99.99 percent, and the purity of the stock seeds obtained through expanding propagation reaches above 99.9 percent.
Description
Technical field
The invention belongs to agricultural technology field, relate to the excellent high-yield breeding method of a kind of guarantor, particularly relate to the excellent high-yield breeding method of guarantor that a kind of two are super hybridization rice parent Guike-2S original seed.
Background technology
Take photo-thermo-sensitive genetic male sterile line as the hybrid rice two-line sterile line of material, with its combo freely, one is dual-purpose, simplifies the advantages such as breeding program and demonstrates good application prospect.At present, the photo-thermo-sensitive genetic male sterile line Peiai 64S of incubation, strain 1S, Y58S and Guangzhan 63S etc. large-area applications in production.But hybrid rice two-line sterile line is subject to light and temperature, and impact causes transformation to cause mixing of male-sterile seed, brings great risk to the two-line hybrid rice production of hybrid seeds.For reducing the potential risk that the two-line sterile line production of hybrid seeds exists, obtaining highly purified two-line sterile line is the important leverage of evading this risk.
The acquisition of highly purified two-line sterile line seed, except selection is except Production Zones Suitable breeding, reinforcement removal of impurities work, also needs the source from sterile line propagation and its purification technique of male sterile line breeder's stock breeding research.
Summary of the invention
The object of this invention is to provide the excellent high-yield breeding method of guarantor that a kind of two are super hybridization rice parent Guike-2S original seed, comprise following step:
Steps A: Guike-2S large group phenotypic screen: the Guike-2S colony of plantation individual plant, from seedling stage to panicle spike primordium differentiation stage, strictly remove hybrid strain, weak tendency strain and surging strain, the intermediate form plant that reservation plant and leaf shape, plant height are consistent with tillering ability;
Step B: Guike-2S sterile site-specific labeling detect: select steps A to obtain the intermediate form plant with Guike-2S characteristic feature through phenotypic screen; select the sterile site-specific labeling PMS3 of two-line sterile line to detect, eliminate the plant do not conformed to specific marker banding pattern;
Step C: Guike-2S finger-print Markers for Detection: select the plant that step B obtains after specific marker screening, detect with 24 marks building fingerprint image, obtain and mark the identical plant of collection of illustrative plates with 24, eliminate the plant incomplete same with its collection of illustrative plates;
Step D: Guike-2S first time climatic cabinate low temperature screening: when Guike-2S ear differentiation fourth stage is, select step C and the potted plant rear immigration climatic cabinate of the identical plant of its finger-print to carry out low temperature screening;
Step e: Guike-2S second time climatic cabinate low temperature screening: the plant after the phytotron low temperature treatment described in step D is shifted out, maturing stage removal has solid plant, not solid plant carries out cutting root and stem of certain plants process makes it regenerate, the regeneration seedlings of cereal crops after cultivating in ear differentiation the
phase moves into climatic cabinate again and carries out low temperature treatment, and after process, plant shifts out climatic cabinate, and maturing stage results solid good plant seed, i.e. Guike-2S breeder's stock, cuts the root and stem of certain plants again by the plant after results seed, and being transferred to Hainan, to carry out the winter numerous;
Step F: standing grain pocket Hainan in Winter is bred, transplants to the Sanya, Hainan area winter numerous by the Guike-2S again cutting the root and stem of certain plants, and time ripe, results seed, namely obtains Guike-2S breeder's stock.
The present invention adopts above technical scheme, its advantage is, first its key is the breeding of high purity raw original seed, comprises large group phenotypic screen, sterile locus specificity Markers for Detection, finger-print Markers for Detection, the screening of phytotron low temperature and Hainan in Winter breeding; Next is the High-yielding Multiplication Techniques of original seed.By the enforcement of these technology, the highly purified Guike-2S original seed of final acquisition.
Preferably, in described step D, the condition of low temperature screening is: the phytotron setting duration of day is 13.5h, and temperature 23.0 DEG C, the processing time is 10 days.
Preferably, in described step e, setting illumination length is 11.0h, temperature 22.0 DEG C, and the processing time is 10 days.
Preferably, in described step F, choosing will be cut root and stem of certain plants plant one strain and is divided into three parts and plant.
Preferably, following step is also comprised:
Step G: the breeder's stock that step e, step F obtain is selected to breed at Yunnan E Shan, sterilized and vernalization by the seed-soaking obtained, light seeding is even to be broadcast, and builds film thermal insulating cold-proof;
Step H: select good, the front breeding field of making non-rice cultivation of isolation, carry out removal of impurities work;
Step I: Guike-2S rice shoot is transplanted in good time, individual plant transplanting;
Step J: strengthen water and fertilizer management, nurturing staff, attention control damage by disease and insect.
The present invention adopts above technical characteristic further, its advantage is, through for many years, multiple spot and multiple season be to the breeding of Guike-2S, think that Sanya, Hainan, Guangxi Gold show high altitude localities, E Shanxian high altitude localities, Yunnan are the Production Zones Suitables that Guike-2S high yield is bred, E Shanxian high altitude localities, Yunnan is the best region of Guike-2S breeding.Therefore, breeder's stock step 4, step 5 obtained is selected to breed at Yunnan E Shan.Yunnan E Shanxian 1-7 month daily mean temperature is not higher than 23 DEG C, and therefore Guike-2S breeding is generally in sowing mid-April, and at the beginning of by the end of June to 7 month, Guike-2S enters the tricky time of low temperature transformation, ears by the end of July.
Preferably, in step G, sow when seed shows money or valuables one carries unintentionally or bud grows 1 cun.
Preferably, in step J, breeding field uses sufficient farmyard manure before transplanting or base fertilizer made by superphosphate, transplants and imposes 82.5 kg/hm in 5 days
2striking root fertilizer made by urea; Transplant and within 9 days, impose urea 150 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2attack fetilizer for tillering; Ear differentiation III ~ IV phase, execute urea 60 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2make ear manuer, promote the strong growth of young fringe.
Preferably, in step J, dry field when individual plant grows 10-14 tillered nursery plant, fills with deep water during main fringe ear differentiation second stage, and the maturing stage keeps land for growing field crops dry wet, overboard harvesting after yellow maturity.
Preferably, in step I, transplant during leaf age 6.0, transplanting spacing is 7 × 5 cun.
Preferably, in step H, breeding can not plant other rice varieties within 300 meters, land for growing field crops, carries out 2-3 removal of impurities respectively in seedling stage, heading stage and maturing stage.
The invention has the beneficial effects as follows: adopt the solution of the present invention, the present invention a kind of two is that the excellent high-yield breeding method of guarantor of super hybridization rice parent Guike-2S original seed is by strict large group phenotypic screen, sterile locus specificity molecular labeling and finger-print Markers for Detection obtain highly purified Guike-2S breeder's stock, again the breeder's stock obtained is selected the region of optimum breeding, the original seed of high-purity and high yield is obtained by high-yield culture technique, original seed is bred further the high-purity parental seed obtaining and the production of hybrid seeds is applied, this has very important meaning to the development of two-line hybrid rice.And through test, the purity of the breeder's stock obtained reaches more than 99.99%, and expand numerous acquisition original seed purity reach more than 99.9%.
Embodiment
Below preferably embodiment of the present invention is described in further detail:
Embodiment 1
1, Guike-2S large group phenotypic screen.Base tested by Nanning paddy rice in 2011, sowed at the beginning of 3 months, selected isolation good area, the Guike-2S colony of plantation about 2000 individual plants.According to the feature of Guike-2S, from seedling stage to panicle spike primordium differentiation stage, strictly remove hybrid strain, weak tendency strain and surging strain, retain the intermediate form plant that plant and leaf shape, plant height and tillering ability etc. are consistent.
2, the sterile site-specific labeling of Guike-2S detects.Select step 1 to obtain intermediate form plant 200 strain with Guike-2S characteristic feature through phenotypic screen, select the sterile site-specific labeling PMS3 of two-line sterile line to detect, eliminate the plant do not conformed to specific marker banding pattern.
3, Guike-2S finger-print Markers for Detection.The 80-120 plant selecting step 2 to obtain after specific marker screening, detects with 24 marks building fingerprint image, obtains and marks the identical plant of collection of illustrative plates with 24, eliminates the plant incomplete same with its collection of illustrative plates.
4, Guike-2S climatic cabinate low temperature screening.Guike-2S ear differentiation fourth stage is the tricky time of sterile changeover.Now, step is selected (3) to carry out low temperature screening with the potted plant rear immigration climatic cabinate of the identical plant of its finger-print about 50 strain.Under the Guike-2S long-day (>=13.5h), transformation temperature is lower than 23.0 DEG C, and the phytotron setting duration of day is 13.5h, temperature 23.0 DEG C, continuously process 10 days.Plant after climatic cabinate low temperature treatment shifts out, and by Common Cultivation measure management, maturing stage removal has solid plant, and not solid plant carries out cutting root and stem of certain plants process makes it regenerate.The regeneration seedlings of cereal crops strengthen water and fertilizer management, and nurturing staff, moves into phytotron again in the ear differentiation IV phase and carry out low temperature treatment, and setting illumination length is 11.0h, temperature 22.0 DEG C, processes 10 days.After process, plant shifts out climatic cabinate, strengthens water and fertilizer management and the extermination of disease and insect pest, in the maturing stage solid good plant seed of results (Guike-2S breeder's stock), eliminates shaky or solid poor plant.Plant after results seed is cut the root and stem of certain plants again, and being transferred to Hainan, to carry out the winter numerous.
5, standing grain pocket Hainan in Winter breeding.At the beginning of mid-November to 12 in the same year, the Guike-2S again cutting the root and stem of certain plants is transplanted to the Sanya, Hainan area winter numerous, select the good field of isolation, for obtaining more plant, root and stem of certain plants plant one point three parts will be cut plant, strengthen water and fertilizer management, nurturing staff, deep water is emerged, shallow water is short tillers, and in time growing 2-3 root regeneration seedling, topdresses in time, strengthen the extermination of disease and insect pest, results seed (Guike-2S breeder's stock) time ripe.
6, select Production Zones Suitable and breed Guike-2S breeder's stock season.Through for many years, multiple spot and multiple season be to the breeding of Guike-2S, think that Sanya, Hainan, Guangxi Gold show high altitude localities, E Shanxian high altitude localities, Yunnan are the Production Zones Suitables that Guike-2S high yield is bred, E Shanxian high altitude localities, Yunnan is the best region of Guike-2S breeding.Therefore, breeder's stock step 4, step 5 obtained is selected to breed at Yunnan E Shan.Yunnan E Shanxian 1-7 month daily mean temperature is not higher than 23 DEG C, and therefore Guike-2S breeding is generally in sowing mid-April, and at the beginning of by the end of June to 7 month, Guike-2S enters the tricky time of low temperature transformation, ears by the end of July.
7, the seed-soaking that step 4, step 5 obtain is sterilized and vernalization, and seed shows money or valuables one carries unintentionally or bud is about about 1 cun sowing, and light seeding is even to be broadcast, and builds film thermal insulating cold-proof.
8, select good, the front breeding field of making non-rice cultivation of isolation, carry out removal of impurities work.The field of non-rice cultivation is made in breeding stock field before should selecting, other rice varieties can not be planted within requiring breeding 300 meters, land for growing field crops, carry out 2-3 removal of impurities respectively in seedling stage, heading stage and maturing stage, mainly remove special-shaped strain, fall field paddy strain, weak tendency strain and solid poor plant etc.
9, transplant, individual plant transplanting in good time.During owing to transplanting, temperature is lower, and the temperature difference is comparatively large sooner or later, and therefore transplant as good during Guike-2S seedling leaf 6.0, strict individual plant transplanting during transplanting, transplanting spacing is 7 × 5 cun.
10, water and fertilizer management is strengthened, nurturing staff, attention control damage by disease and insect.Breeding field uses sufficient farmyard manure before transplanting or base fertilizer made by superphosphate, transplants and imposes 82.5 kg/hm in about 5 days
2striking root fertilizer made by urea; Transplant and within about 9 days, impose urea 150 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2attack fetilizer for tillering; Ear differentiation III ~ IV phase, execute urea 60 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2make ear manuer, promote the strong growth of young fringe.Water and fertilizer management: very little water is turned green, shallow water is short tillers, timely dry field when individual plant grows 11-14 tillered nursery plant, and fill with deep water (keeping land for growing field crops low temperature) during main fringe ear differentiation second stage in time, the maturing stage keeps land for growing field crops dry wet, overboard harvesting after yellow maturity; The extermination of disease and insect pest: attention control rice blast, banded sclerotial blight, the damage by disease and insect such as false smut and rice fulgorid.
11, gather in time, prevent from mixing.Breed at Yunnan E Shan, time ripe, the temperature difference is comparatively large sooner or later, will gather in time, prevent grain osses time medium well; Breeding stock area is little, adopts hand harvest during harvesting, removes threshing machine, threshing ground is clean, prevent from mixing.
Record through experiment, the purity through the breeder's stock of step 1-5 acquisition reaches more than 99.99%, and the original seed purity expanding numerous acquisition through step 6-11 reaches more than 99.9%.
Embodiment 2
1, male sterile line large group phenotypic screen.Select isolation good area, the Guike-2S colony of plantation about 2000 individual plants.According to the feature of Guike-2S, from seedling stage to panicle spike primordium differentiation stage, strictly remove hybrid strain, weak tendency strain and surging strain, retain the intermediate form plant that plant and leaf shape, plant height and tillering ability etc. are consistent.
2, the sterile site-specific labeling of male sterile line detects.Select step 1 to obtain intermediate form plant 200 strain with Guike-2S characteristic feature through phenotypic screen, select the sterile site-specific labeling PMS3 of two-line sterile line to detect, the plant do not conformed to specific marker banding pattern eliminated by pottery.Guike-2S sterile site-specific labeling PMS3 design principle (
reference: Jihua Ding; Qing Lu; Yidan Ouyang; Hailiang Mao; Pingbo Zhang; Jialing Yao; Caiguo Xu; Xianghua Li; Jinghua Xiao; Qifa Zhang.A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice.Proceedings of the National Academy of Sciences, 2012,109 (7): 2654-2659).
3, male sterile line finger-print Markers for Detection.The structure of Guike-2S finger-print: choose 24 paddy rice SSR marker, on every bar chromosome 2.Wherein first-selected mark 12, is respectively RM297, RM71, RM85, RM5414, RM274, RM190, RM336, RM337, RM219, RM311, RM209 and RM17; Candidate marks 12, is RM1195, RM208, RM232, RM273, RM267, RM253, RM18, RM72, RM278, RM258, RM224 and RM19(respectively
reference: Zhuan Jieyun, Shi Yongfeng, Ying Jiezheng etc. the Primary Construction of the main kind microsatellite marker database that transplants rice seedlings of China. rice in China science, 2006,20(5): 460-468).Extract the STb gene of Guike-2S, pcr amplification, electrophoretic separation amplified production are carried out to 24 marks, obtain Guike-2S fingerprint (banding pattern), finger-print.The 80-120 plant selecting step 2 to obtain after specific marker screening, detects with 24 marks building fingerprint image, obtains and marks the identical plant of collection of illustrative plates with 24, eliminates the plant incomplete same with its collection of illustrative plates.
4, male sterile line climatic cabinate low temperature screening.The Guike-2S ear differentiation IV phase is the tricky time of sterile changeover.Now, step 3 and the potted plant rear immigration climatic cabinate of the identical plant of its finger-print about 50 strain is selected to carry out low temperature screening.Under the Guike-2S long-day (>=13.5h), transformation temperature is lower than 23.0 DEG C, and the phytotron setting duration of day is 13.5h, temperature 23.0 DEG C, continuously process 10 days.Plant after phytotron low temperature treatment shifts out, and by Common Cultivation measure management, maturing stage removal has solid plant, and not solid plant carries out cutting root and stem of certain plants process makes it regenerate.The regeneration seedlings of cereal crops strengthen water and fertilizer management, and nurturing staff, moves into climatic cabinate again in the ear differentiation IV phase and carry out low temperature treatment, and setting illumination length is 11.0h, temperature 22.0 DEG C, processes 10 days.After process, plant shifts out climatic cabinate, strengthens water and fertilizer management and the extermination of disease and insect pest, in the maturing stage solid good plant seed of results (Guike-2S breeder's stock), eliminates shaky or solid poor plant.Plant after results seed is cut the root and stem of certain plants again, and being transferred to Hainan, to carry out the winter numerous.
5, standing grain pocket Hainan in Winter breeding.At the beginning of mid-November to 12, the Guike-2S again cutting the root and stem of certain plants is transplanted to the Sanya, Hainan area winter numerous, select the good field of isolation, for obtaining more plant, root and stem of certain plants plant one point three parts will be cut plant, add pipe water and fertilizer management, nurturing staff, deep water is emerged, shallow water is short tillers, and in time growing 2-3 root regeneration seedling, topdresses in time, strengthen the extermination of disease and insect pest, results seed (Guike-2S breeder's stock) time ripe.
6, select Production Zones Suitable and breed Guike-2S breeder's stock season.Through for many years, multiple spot and multiple season be to the breeding of Guike-2S, think that Sanya, Hainan, Guangxi Gold show high altitude localities, E Shanxian high altitude localities, Yunnan are the Production Zones Suitables that Guike-2S high yield is bred, E Shanxian high altitude localities, Yunnan is the best region of Guike-2S breeding.Therefore, breeder's stock step 4, step 5 obtained is selected to breed at Yunnan E Shan.Yunnan E Shanxian 1-7 month daily mean temperature is not higher than 23 DEG C, and therefore Guike-2S breeding is generally in sowing mid-April, and at the beginning of by the end of June to 7 month, Guike-2S enters the tricky time of low temperature transformation, ears by the end of July.
7, the seed-soaking that step 4, step 5 obtain is sterilized and vernalization, and seed shows money or valuables one carries unintentionally or bud is about about 1 cun sowing, and light seeding is even to be broadcast, and builds film thermal insulating cold-proof.
8, select good, the front breeding field of making non-rice cultivation of isolation, carry out removal of impurities work.The field of non-rice cultivation is made in breeding stock field before should selecting, other rice varieties can not be planted within requiring breeding 300 meters, land for growing field crops, carry out 2-3 removal of impurities respectively in seedling stage, heading stage and maturing stage, mainly remove special-shaped strain, fall field paddy strain, weak tendency strain and solid poor plant etc.
9, transplant, individual plant transplanting in good time.During owing to transplanting, temperature is lower, and the temperature difference is comparatively large sooner or later, and therefore transplant as good during Guike-2S seedling leaf 6.0, strict individual plant transplanting during transplanting, transplanting spacing is 7 × 5 cun.
10, water and fertilizer management is strengthened, nurturing staff, attention control damage by disease and insect.Breeding field uses sufficient farmyard manure before transplanting or base fertilizer made by superphosphate, transplants and imposes 82.5 kg/hm in about 5 days
2striking root fertilizer made by urea; Transplant and within about 9 days, impose urea 150 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2attack fetilizer for tillering; Ear differentiation III ~ IV phase, execute urea 60 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2make ear manuer, promote the strong growth of young fringe.Water and fertilizer management: very little water is turned green, shallow water is short tillers, timely dry field when individual plant grows 10-14 tillered nursery plant, and fill with deep water (keeping land for growing field crops low temperature) during main fringe ear differentiation second stage in time, the maturing stage keeps land for growing field crops dry wet, overboard harvesting after yellow maturity; The extermination of disease and insect pest: the damage by disease and insect such as attention control rice blast, banded sclerotial blight, false smut and rice fulgorid.
11, gather in time, prevent from mixing.Breed at Yunnan E Shan, time ripe, the temperature difference is comparatively large sooner or later, will gather in time, prevent grain osses during 8 maturation; Breeding stock area is little, adopts hand harvest during harvesting, removes threshing machine, threshing ground is clean, prevent from mixing.
Record through test, the purity through the breeder's stock of step 1-5 acquisition reaches more than 99.99%, and the original seed purity expanding numerous acquisition through step 6-11 reaches more than 99.9%.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (5)
1. two is the excellent high-yield breeding method of guarantor of super hybridization rice parent Guike-2S original seed, it is characterized in that, comprises following step:
Steps A: Guike-2S large group phenotypic screen: the Guike-2S colony of plantation individual plant, from seedling stage to panicle spike primordium differentiation stage, strictly removes hybrid strain, weak tendency strain and surging strain, the intermediate form plant that reservation plant and leaf shape, plant height are consistent with tillering ability;
Step B: Guike-2S sterile site-specific labeling detect: select steps A to obtain the intermediate form plant with Guike-2S characteristic feature through phenotypic screen; select the sterile site-specific labeling PMS3 of two-line sterile line to detect, eliminate the plant do not conformed to specific marker banding pattern;
Step C: Guike-2S finger-print Markers for Detection: select the plant that step B obtains after specific marker screening, detect with 24 marks building fingerprint image, obtain and mark the identical plant of collection of illustrative plates with 24, eliminate the plant incomplete same with its collection of illustrative plates;
Wherein, the structure of Guike-2S finger-print: choose 24 paddy rice SSR marker, on every bar chromosome 2, first-selected mark 12, is respectively RM297, RM71, RM85, RM5414, RM274, RM190, RM336, RM337, RM219, RM311, RM209 and RM17; Candidate marks 12, is RM1195, RM208, RM232, RM273, RM267, RM253, RM18, RM72, RM278, RM258, RM224 and RM19 respectively;
Step D: Guike-2S first time climatic cabinate low temperature screening: when Guike-2S ear differentiation fourth stage, select step C and the potted plant rear immigration climatic cabinate of the identical plant of its finger-print to carry out low temperature screening;
Step e: Guike-2S second time climatic cabinate low temperature screening: the plant after the phytotron low temperature treatment described in step D is shifted out; maturing stage removal has solid plant; not solid plant carries out cutting root and stem of certain plants process makes it regenerate; the regeneration seedlings of cereal crops move into climatic cabinate again in the ear differentiation IV phase and carry out low temperature treatment after cultivating; after process, plant shifts out climatic cabinate; the solid good plant seed of maturing stage results; i.e. Guike-2S breeder's stock; plant after results seed is cut the root and stem of certain plants again, and being transferred to Hainan, to carry out the winter numerous;
Step F: standing grain pocket Hainan in Winter is bred, transplants to the Sanya, Hainan area winter numerous by the Guike-2S again cutting the root and stem of certain plants, and time ripe, results seed, namely obtains Guike-2S breeder's stock;
Step G: the breeder's stock that step e, step F obtain is selected to breed at Yunnan E Shan, sterilized and vernalization by the seed-soaking obtained, light seeding is even to be broadcast, and builds film thermal insulating cold-proof;
Step H: select isolation breeding field that is good, non-rice cultivation in earlier stage, carry out removal of impurities work;
Step I: Guike-2S rice shoot is transplanted in good time, individual plant transplanting;
Step J: strengthen water and fertilizer management, nurturing staff, attention control damage by disease and insect, breeding field uses sufficient farmyard manure before transplanting or base fertilizer made by superphosphate, transplants and imposes 82.5 kg/hm in 5 days
2striking root fertilizer made by urea; Transplant and within 9 days, impose urea 150 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2attack fetilizer for tillering; Ear differentiation III ~ IV phase, execute urea 60 kg/hm
2, composite fertilizer 225 kg/hm
2, potassium chloride 150 kg/hm
2make ear manuer, promote the strong growth of young fringe;
In step J, dry field when individual plant grows 10-14 tillered nursery plant, deep water is filled with during main fringe ear differentiation second stage, maturing stage keeps land for growing field crops dry wet, overboard harvesting after yellow maturity, in described step D, the condition of low temperature screening is: the phytotron setting duration of day is 13.5h, temperature 23.0 DEG C, the processing time is 10 days;
In described step e, setting illumination length is 11.0h, temperature 22.0 DEG C, processes 10 days.
2. as claimed in claim 1 two is the excellent high-yield breeding method of guarantor of super hybridization rice parent Guike-2S original seed, it is characterized in that, in described step F, will cut root and stem of certain plants plant one point three parts and plant.
3. as claimed in claim 1 two is the excellent high-yield breeding method of guarantor of super hybridization rice parent Guike-2S original seed, it is characterized in that, in step G, seed show money or valuables one carries unintentionally or long 1 cun of bud time sowing.
4. as claimed in claim 1 two is the excellent high-yield breeding method of guarantor of super hybridization rice parent Guike-2S original seed, it is characterized in that, in step I, transplant during leaf age 6.0, transplanting spacing is 7 × 5 cun.
5. as claimed in claim 1 two is the excellent high-yield breeding method of guarantor of super hybridization rice parent Guike-2S original seed, it is characterized in that, in step H, breeding can not plant other rice varieties within 300 meters, land for growing field crops, carries out 2-3 removal of impurities respectively in seedling stage, heading stage and maturing stage.
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| CN1994059A (en) * | 2006-01-06 | 2007-07-11 | 云南农业大学 | Three-step method for purification of Dian-1 type japonica sterile line stock seed |
| CN102334449A (en) * | 2010-07-22 | 2012-02-01 | 湖南杂交水稻研究中心 | Method for continuously batch purifying and breeding paddy rice low thermo-sensitive genic male sterile original seed |
| CN101999302B (en) * | 2010-10-19 | 2011-12-28 | 湖北省农业科学院粮食作物研究所 | Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines |
| CN102498998B (en) * | 2011-11-24 | 2013-09-18 | 湖南杂交水稻研究中心 | Method for purification, retainment and propagation of seeds of rice photo-thermo-sensitive male sterile line |
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2013
- 2013-11-25 CN CN201310599541.6A patent/CN103609434B/en not_active Expired - Fee Related
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