Summary of the invention
The object of the present invention is to provide a kind of moisturizing whitening cream that natural biological extract is active component and preparation method thereof of take.
The invention provides a kind of black Fructus Lycii moisturizing whitening cream, it is that supplementary material by following weight percentage is prepared from:
Further, it is that supplementary material by following weight percentage is prepared from:
In the present invention, multiple natural extract is used in combination, has brought into play more good skin care activity, for market provides a kind of skin effect better, safe skin care item.
Wherein, described black Fructus Lycii seed oil is to pass through supercritical CO
2extraction gained, its preparation method is: take black Fructus Lycii seed 400g and pack into and in 2L extraction kettle, carry out supercritical CO
2extraction, opens CO
2gas tank, pressurizes to extraction kettle, when extraction kettle pressure reaches required pressure, opens the pressure valve adjusting seamless power of controlling separating still, regulates CO simultaneously
2flow, and keep temperature and pressure, then every 15min, collect single extraction thing, to weigh, black Fructus Lycii seed oil is collected by two-stage separation kettle, CO
2can be recycled.
Wherein, described black Fructus Lycii fruit powder extracting solution is the water extract of black Fructus Lycii fruit powder; Preferably, the preparation method of black Fructus Lycii extracting solution is as follows: precision takes the dried powder of 5g black Fructus Lycii, add 50mL deionized-distilled water, in 50 ℃ of water-baths, extract 2 times, each 15 minutes, after filtration, residue is added to water 100mL extracts 2 times at 75 ℃, each 15 minutes, merge extracted twice liquid, filter, be evaporated to 10g/mL, obtain black Fructus Lycii extracting solution.
Wherein, the preparation method of yeast extract is as follows: by beer yeast washing impurity-removing matter, debitterize in 0-4 ℃ of water, carry out again coarse filtration, obtain the yeast slurry containing solid content, then add promoter, plasmolyticum and NaOH, regulate pH to 6.0~7.0, at 52 ℃ ± 2 ℃ temperature, 2-2.5 hour is extracted in self-dissolving, boil, enzyme denaturing, cooling standing, centrifugalize, remove cell wall fragments, vacuum-concentrcted to concentration is 10g/mL, obtains yeast extract.
Wherein, the preparation method of seaweed extracted liquor is as follows: the Sargassum after the removal of impurity is cleaned with the dilute acid soln that pH is 2~4, rub, and grind homogenate in the dilute acid soln that is 2~4 at pH, then by the Sargassum after homogenate 121~135 ℃ of temperature, under pressure 1~5MPa, ripening sterilizing is 0.5~2 hour, Sargassum slurry after degraded adds alkali and adjusts pH to 5.0-7.0, to the cultured probiotic bacteria strain normal fermentation in advance of inoculation in the Sargassum serosity after adjusting, again to fermentation after serosity in add algin oligosaccharide to allocate, probiotic bacteria is protected, propagation, be deployed into the serosity that concentration is 20g/mL, be seaweed extracted liquor.
But the extracting method of various extracts is not limited to said method, the disclosed extracting method of manufacturing cosmetics-stage extract of all prior aries is all applicable to the present invention.
Wherein, described emulsifying agent is selected from one or more in sorbester p18, the stearic alcohol ether in polyoxyethylene-2 (21), polysorbate60, SS-5051, GLUCATE SS, Glucamate SSE-20, emulsifying agent M68.
Wherein, described antibacterial is selected from one or more in Kathon CG, Germall II, Germall Plus, Glydant PLUS.
The present invention also provides the preparation method of above-mentioned black Fructus Lycii moisturizing whitening cream, and it comprises following operating procedure:
(1) take by weight ratio raw material;
(2) by cetostearyl alcohol, molecular distillation monoglyceride, sorbester p18, polysorbate60, emulsifying agent, liquid paraffin, caprylic/capric triglyceride, polydimethylsiloxane, vaseline, propylparaben heated and stirred in the water-bath of 85 ℃ ± 5 ℃, obtain oil-phase solution;
(3) by disodiumedetate, glycerol, 1,3 butylene glycol, allantoin, methyl hydroxybenzoate and water, the stirred in water bath at 85 ℃ ± 5 ℃ makes to dissolve, and obtains aqueous phase solution;
(4) oil-phase solution is slowly joined in aqueous phase solution, in 85 ℃ ± 5 ℃ water-baths, emulsifying is 5~8 minutes, then adds emulsifying agent match Bick 305 to continue emulsifying 3~5 minutes;
(5) continue to stir, when thing to be mixed is cooled to 40~50 ℃, add black Fructus Lycii seed oil, vitamin E, black Fructus Lycii fruit powder extracting solution, yeast extract, seaweed extracted liquor, antibacterial and essence, fully stir, make its mix homogeneously, obtain black Fructus Lycii moisturizing whitening cream.
The present invention is strong in order to prepare moisture retention, and the facial cream simultaneously with effects such as whitening, desalination mottle, defying age, Glycerins, adopt black Fructus Lycii seed oil, black Fructus Lycii fruit powder extracting solution, yeast extract, seaweed extracted liquor as main active, and with other material combination, join according to a certain percentage in conventional components of cosmetics and make.
Wherein, in black Fructus Lycii seed oil, be rich in linoleic acid, oleic acid, gamma-Linolenic acid, vitamin E, beta-carotene, phospholipid, and contain various trace elements and bioactive substance epidermal growth factor, superoxide dismutase (SOD) etc.Linoleic acid, gamma-Linolenic acid etc. are all natural unsaturated fatty acids.Vitamin E is a kind of very strong antioxidant; restraint of tyrosinase is active; reduce melanic formation; suppress lipid peroxidation; keep contact between tissue; make skin smooth, flexible; energy Cell protection film is avoided the oxidative damage of free radical; so can help to repair and consolidate the natural protective barrier of skin; pin moisture in skin, provide deep layer from inside to outside to nourish, can also promote the absorption of vitamin C and A; and help cell to absorb better the nutritional labeling of outside skin care item, beauty functions is doubled.Beta-carotene can be promoted human immunity defense function and slow down aging.Superoxide dismutase (SOD) can antiradiation injury, is highly resistant to again peroxidating radical anion, has anti-aging effects.Through a large amount of clinical verifications, boxthorn seed oil has following effect: boxthorn seed oil can reduce plasma cholesterol, reduces cholesterol level in blood vessel wall, prevents blood fat and atherosclerosis; Can promote cutaneous circulation, participate in skin metabolism directly, eliminate oxygen-derived free radicals, delay the aging of epidermis and corium, strengthen skin hydration function, make the soft also high resilience of skin, have obvious whitening effect; Senile plaque and chloasma pigmentation are had to the effect of taking off that significantly disappears.
Black Fructus Lycii extracting solution is the water extract of black Fructus Lycii fruit powder.The composition such as rich in proteins, aminoacid, flavone, procyanidin, fat, polysaccharide, vitamin E in lycium ruthenicum fruit.Protein is the structural material of human body skin, gives skin with nutrition, can make impaired cell, tissue obtain timely high-quality repairing, makes the high resilience of skin gloss, delaying senility.In lycium ruthenicum fruit, contain 17 seed amino acids except tryptophan, aminoacid is form organism protein and with one of closely bound up base substance of vital movement, nutrition can be provided, promote growth, maintain normal metabolism.Assessment of Pigment of Lycium ruthenicum Murr is anthocyanin class and flavone compound, and its non-oxidizability is obviously better than than vitamin E, and visible ray and ultraviolet are had to Stabilization.The non-oxidizability of flavone is mainly manifested in aspects such as removing free radical and anti-peroxidation.
Yeast extract is rich in the material of the active skull cap components such as micromolecular aminoacid, peptide, nucleotide, vitamin, and wherein amino acid content is more than 30%, and total protein is more than 50%, and nucleotide is more than 10%.Nucleic acid and nucleotide are the main hereditary materials of human body, there is enhancing metabolism, improve the effects such as protein synthesis speed, strengthen immune function, enhance SOD activity, improve skin antiradical activities, skin-nourishing is provided, adjust sebum secretion, promote collagen protein synthesis, smooth away wrinkles, isolation radiation, prevents ultra-violet absorption.In addition, in product, the nutrient such as a small amount of vitamin and mineral can also provide sufficient nutrition for skin.
Seaweed extracted liquor is a kind of marine organisms product of pure natural, include abundant algin acid, crude protein, multivitamin, enzyme, trace element etc., these nutrients can reduce skin surface blood fat after skin absorbs, promote the hemopoietic function of epidermis cell, can produce bacteriostasis to cell in vitro, also there is moisturizing, smooth function.In addition, a large amount of aniones that contain, can stimulate fibrocyte to generate collagen protein and elastin laminin, promote the metabolism of skin, have given its crease-resistant, antidotal effect.
Moisturizing whitening cream of the present invention, effectively moisturizing moisturizing, horny layer softening, freckle removing and whitening, infiltration reparation, significantly improve skin inwardness aging, prevent the problems such as the wrinkling and mottle of skin, also there is Promote cell's growth, absorb ultraviolet, anti-ageing effect of waiting for a long time, waking up skin vigor, give the moisture feeling of skin water profit and abundant reflecting feel, and coating is felt, assimilation effect is excellent.The present invention is made by pure natural substance, does not add pigment, containing ethanol, gentleness, does not stimulate, has no side effect.
Below pass through the specific embodiment; scope of the present invention is further elaborated and is illustrated; but protection scope of the present invention is not limited to this; any those of ordinary skill in the art are in the disclosed technical scope of the present invention; can be without modification or the replacement of creative work, within all should being encompassed in protection scope of the present invention.
The specific embodiment
The preparation method of yeast extract is as follows: by beer yeast washing impurity-removing matter, debitterize in 0-4 ℃ of water, carry out again coarse filtration, obtain the yeast slurry containing solid content, then add promoter, plasmolyticum and NaOH, regulate pH to 6.0~7.0, at 52 ℃ ± 2 ℃ temperature, 2-2.5 hour is extracted in self-dissolving, then boils, enzyme denaturing, cooling standing, centrifugalize, removes cell wall fragments, vacuum-concentrcted to concentration is 10g/mL, obtains yeast extract.
The preparation method of seaweed extracted liquor is as follows: the Sargassum after the removal of impurity is cleaned with the dilute acid soln that pH is 2~4, rub, and grind homogenate in the dilute acid soln that is 2~4 at pH, then by the Sargassum after homogenate 121~135 ℃ of temperature, under pressure 1~5MPa, ripening sterilizing is 0.5~2 hour, Sargassum slurry after degraded adds alkali and adjusts pH to 5.0-7.0, to the cultured probiotic bacteria strain normal fermentation in advance of inoculation in the Sargassum serosity after adjusting, again to fermentation after serosity in add algin oligosaccharide to allocate, probiotic bacteria is protected, propagation, be deployed into the serosity that concentration is 20g/mL, be seaweed extracted liquor.
The black Fructus Lycii seed oil using in the present invention is the general raw material of cosmetic industry, can on cosmetic material market, buy, and also can obtain with reference to prior art preparation, for example: pass through supercritical CO
2extraction is obtained, and its concrete preparation method can be: take black Fructus Lycii seed 400g and pack into and in 2L extraction kettle, carry out supercritical CO
2extraction, opens CO
2gas tank, pressurizes to extraction kettle, when extraction kettle pressure reaches required pressure, opens the pressure valve adjusting seamless power of controlling separating still, regulates CO simultaneously
2flow, and keep temperature and pressure, then every 15min, collect single extraction thing, to weigh, black Fructus Lycii seed oil is collected by two-stage separation kettle, CO
2can be recycled.Black Fructus Lycii seed oil also can be extracted and be obtained by modes such as squeezings.
The black Fructus Lycii extracting solution that the present invention uses is the water extract of black Fructus Lycii fruit powder, its concrete preparation method can be: precision takes the dried powder of 5g black Fructus Lycii, add 50mL deionized-distilled water, in 50 ℃ of water-baths, extract 2 times, each 15 minutes, after filtration, residue is added to water 100mL extracts 2 times at 75 ℃, each 15 minutes, merge extracted twice liquid, filter, be evaporated to 10g/mL, obtain black Fructus Lycii extracting solution.
Concentration after concentrated in the present invention is all with the mass/volume of concentrated solution, to calculate the ratio of gained.
In the embodiment of the present invention, each component is listed in table 1, and proportional quantity is with mass percent example.
Table 1
The preparation method of the embodiment of the present invention is all according to following steps:
(1) take by weight ratio raw material;
(2) by cetostearyl alcohol, molecular distillation monoglyceride, sorbester p18, polysorbate60, emulsifying agent, liquid paraffin, caprylic/capric triglyceride, polydimethylsiloxane, vaseline, propylparaben heated and stirred in the water-bath of 85 ℃ ± 5 ℃, obtain oil-phase solution;
(3) by disodiumedetate, glycerol, 1,3 butylene glycol, allantoin, methyl hydroxybenzoate and water, the stirred in water bath at 85 ℃ ± 5 ℃ makes to dissolve, and obtains aqueous phase solution;
(4) oil-phase solution is slowly joined in aqueous phase solution, in 85 ℃ ± 5 ℃ water-baths, emulsifying is 5~8 minutes, then adds emulsifying agent match Bick 305 to continue emulsifying 3~5 minutes;
(5) continue to stir, when thing to be mixed is cooled to 40~50 ℃, add black Fructus Lycii seed oil, vitamin E, black Fructus Lycii fruit powder extracting solution, yeast extract, seaweed extracted liquor, antibacterial and essence, fully stir, make its mix homogeneously, obtain black Fructus Lycii moisturizing whitening cream.
Embodiment 8
The Detection of Stability of the embodiment of the present invention 5 products:
1, pH value is 6.4.
2, heat resistant test, puts into calorstat (40 ± 1) ℃ the sample of Packing Intact and keeps 24h, returns to after room temperature without oil-water separation phenomenon.
3, low temperature resistant test, puts into refrigerator-5~-10 ℃ the sample of Packing Intact and places 24h, returns to after room temperature without layering, coarse, metachromatism.
4, centrifugal test, places in centrifuge the sample of Packing Intact with 2000 revs/min, and centrifugal 30 minutes, without oil-water separation phenomenon.
As can be seen here, the facial cream of the embodiment of the present invention 5 preparations has good stability.
Embodiment 9 clinical experiment of the present invention and result
Experimental subject: select age bracket aspiration trier 40 people of 32~48 years old, be divided at random 2 groups, test group and matched group, every group of 20 people.
Experimental technique: test group trier carries out clean processing before sleeping every night, is then evenly applied in face by the facial cream of embodiment 5 preparations, and experimental period is one month.Matched group trier carries out clean processing before sleeping every night, then the prepared facial cream of embodiment 1 is evenly applied in to face, and experimental period is one month.Within every 7 days, 15 days, 30 days, add up one time result on trial.
Evaluation criterion: effectively represent that skin water profit is glossy, tender flexible in vain, obviously desalinate mottle, microgroove also makes moderate progress; Invalid representation skin texture with experiment before and be not any change; General expression skin quality makes moderate progress, but effect is not very remarkable, between between the two above-mentioned.
Table 2
Result on trial: after 7 days, the product of being prepared by embodiment 5 has the moisturizing whitening effect that is obviously better than being gone by embodiment the product of preparation, test group 14 examples are effective, effective percentage 70%, and matched group 9 examples, effective percentage 45%; After 15 days, test group 18 examples are effective, effective percentage 90%, and matched group 12 examples are effectively, effective percentage 60%; After 30 days, test group 20 examples are effective, effective percentage 100%, and matched group 15 examples are effectively, effective percentage 75%.Test group trier's skin has obviously increased glossiness, and fine and smooth water profit, also has whitening function, desalination mottle.Do not have an example to occur the reactions such as skin allergy, discomfort.
As can be seen here, the product of being prepared by embodiment 5, effect is excellent.