CN103558074B - Cell-stain apparatus - Google Patents
Cell-stain apparatus Download PDFInfo
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- CN103558074B CN103558074B CN201310470832.5A CN201310470832A CN103558074B CN 103558074 B CN103558074 B CN 103558074B CN 201310470832 A CN201310470832 A CN 201310470832A CN 103558074 B CN103558074 B CN 103558074B
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Abstract
Cell-stain apparatus, comprises, frame; Multiple dyed plate, all dismountablely on each dyed plate is provided with multiple dyeing storehouse; Be arranged on the conveying assembly for driving multiple dyed plate to move at the length direction of frame of frame lower; Movable to be arranged in frame and two reagent rack be positioned at above conveying assembly; Drive two driver elements that two reagent rack are moved at the length direction of frame respectively; Be movably arranged on the erecting frame in frame, the bottom of this erecting frame is provided with multiple transfer pipet along the arrangement of frame Width, and the upper end open of transfer pipet is communicated with rack-mounted air pump by flexible pipe; For driving erecting frame along the up and down driven unit of the short transverse of frame.Compared to prior art, cell-stain apparatus of the present invention, without the need to people's manual manipulation, can effectively reduce cell sample, the contaminated probability of coloring agent, and can improve the efficiency of cell dyeing; Meanwhile, it is easy to operate, takes up room less.
Description
Technical field
The present invention relates to cell dyeing technical field, be specifically related to a kind of cell-stain apparatus.
Background technology
In medical field, normally to after histocyte dyeing, it is carried out to the detection of disease.Present stage, special cell-stain apparatus is normally adopted to complete the dyeing course of cell.
The automaticity of existing cell-stain apparatus is lower, it in operation, a lot of step all needs to rely on people's manual manipulation, have impact on the efficiency of cell dyeing, further, in operation, the possibility contacted with cell sample, coloring agent etc. is larger for staff, thus increase the contaminated probability such as cell sample and coloring agent, finally cause the detection in later stage and actual conditions to there is deviation.
Summary of the invention
For the deficiencies in the prior art, object of the present invention is intended to provide a kind of cell-stain apparatus, and its automaticity is higher, avoids people's manual manipulation completely, thus improves the efficiency of cell dyeing, reduces the contaminated probability such as cell sample, coloring agent.
For achieving the above object, the present invention adopts following technical scheme:
Cell-stain apparatus, comprises,
Frame;
The dyed plate that multiple bearing of trend is consistent with the Width of frame, all dismountablely on each dyed plate is provided with multiple dyeing storehouse, and the plurality of dyeing storehouse arranges along the length direction of dyed plate;
Be arranged on the conveying assembly for driving multiple dyed plate to move at the length direction of frame of frame lower;
Movable to be arranged in frame and two reagent rack be positioned at above conveying assembly, these two reagent rack are respectively used to placement and histiocytic sample hose and coloring agent are housed, and both it on all have for placing the position, hole of getting liquid rifle head;
Drive two driver elements that two reagent rack are moved at the length direction of frame respectively;
Be movably arranged on the erecting frame in frame, the bottom of this erecting frame is provided with multiple transfer pipet along the arrangement of frame Width, and the upper end open of transfer pipet is communicated with rack-mounted air pump by flexible pipe;
For driving erecting frame along the up and down driven unit of the short transverse of frame.
Conveying assembly comprises,
Be articulated in the main drive shaft of frame length direction one end, main drive shaft extends along the Width of frame, and its two ends are synchronously connected with a drive sprocket respectively;
Be articulated in the driven shaft of the frame length direction other end, driven shaft extends along the Width of frame, and its two ends are synchronously connected with a driven sprocket respectively;
The first motor rotated for driving main drive shaft;
Two driving chains, the drive sprocket of the respectively synchronous winding of this two driving chain in the same side and driven sprocket outside; Driving chain is made up of multiple chain link, a connection piece is provided with between two adjacent chain links, the two ends of web member are hinged with the chain link being positioned at its two ends respectively, and web member is fixed with swivel base, and the two ends of dyed plate are articulated in two swivel bases relative on two driving chains respectively.
The two ends of dyed plate are respectively arranged with an installing plate, the end winding support of dyed plate is inside the bottom of installing plate, the top pars intermedia of two installing plates is provided with outward extending pivot, the center line of two pivots overlaps, and two pivots are installed on two swivel bases relative on two driving chains respectively by bearing.
The front end of frame is provided with a photoelectric sensor, one of them driving chain is provided with a shadow shield corresponding with this photoelectric sensor.
The both sides of frame are respectively arranged with a guide rail extended along frame length direction, and what slided respectively in the both sides of two reagent rack is arranged on two guide rails; Driver element comprises second motor of body partial fixing in frame, be articulated in frame length direction one end and the driving pulley that the second motor in synchrony connects, the driven pulley being articulated in the frame length direction other end, the synchronous belt of synchronous winding in driving pulley and driven pulley outside, and two reagent rack are fixedly connected on the synchronous belt of two driver elements respectively.
This cell-stain apparatus also includes a upset liquid pouring machine structure, this upset liquid pouring machine structure comprises, be arranged on the gear of dyed plate end, movable to be arranged in frame and the orientation of double wedge on it tooth bar consistent with frame length direction, for carry-over bar one and the position of gears meshing and the driving mechanism that moves between one and the gear position of mutually staggering.
Driving mechanism comprises three motor of body partial fixing in frame, to be synchronously connected on the 3rd machine shaft and being socketed in the feed screw nut transmitted on screw mandrel, being arranged in frame and bearing of trend and the consistent line slide rail of frame Width of the bearing of trend drive lead screw consistent with the Width of frame, coupling, and tooth bar is fixedly connected on feed screw nut and goes up and being arranged on line slide rail of sliding.
Driven unit comprises four motor of a body partial fixing in frame, and erecting frame connects with the rotating shaft of the mode of screw rod transmission with the 4th motor.
The cross-under of transfer pipet activity also can be movable relative to erecting frame along the short transverse of frame on erecting frame, the top ends of transfer pipet is provided with the catch for preventing transfer pipet from moving downward relative to erecting frame, transfer pipet is provided with the defining flange be positioned at below catch; Defining flange on each transfer pipet and be provided with a spring between erecting frame, the transfer pipet corresponding with it is pushed up the elastic stress of pressure for providing one by this spring up and down.
The bottom of erecting frame is fixed with multiple sliding sleeve equal with transfer pipet quantity, and transfer pipet activity cross-under is one to one in sliding sleeve, and catch is mounted on the top ends of sliding sleeve, and spring is limited between defining flange and the bottom face of sliding sleeve.
The bottom activity of erecting frame a pressing plate is installed, erecting frame is provided with one the 5th motor, the rotating shaft of the 5th motor to be connected with pressing plate in the mode of screw rod transmission and for moving up and down relative to erecting frame in the short transverse of frame with dynamic pressure plate, pressing plate is provided with the through hole passed for transfer pipet, the periphery of through hole is formed with the top splenium pushing up the end, the rifle crown on pressure transfer pipet when pressing plate moves downward relative to erecting frame downwards.
The width of through hole is greater than the diameter of transfer pipet, is less than the diameter of rifle cephalic par.
Beneficial effect of the present invention is:
Compared to prior art, cell-stain apparatus of the present invention, without the need to people's manual manipulation, can effectively reduce cell sample, the contaminated probability of coloring agent, and can improve the efficiency of cell dyeing; Meanwhile, it is easy to operate, takes up room less.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is inner structure schematic diagram of the present invention;
Fig. 3 is the structural representation of conveying assembly in Fig. 1;
Fig. 4 is the scheme of installation of conveying assembly in Fig. 1;
Fig. 5 is the structural representation of driving chain in Fig. 3;
Fig. 6 is the structural representation that the present invention overturns liquid pouring machine structure;
Fig. 7 is the structural representation of driving mechanism in Fig. 6;
Fig. 8 is the working state schematic representation overturning liquid pouring machine structure in Fig. 6;
The structural representation of Fig. 9 reagent rack of the present invention;
Figure 10 is that the present invention takes arms the structural representation of a mechanism;
Figure 11 is the zoomed-in view at A place in Figure 10;
Figure 12 is the B-B zoomed-in view of Figure 11;
Figure 13 is the structural representation of Tuo Qiangtou mechanism of the present invention;
Figure 14 is the scheme of installation of Figure 13 Zhong Tuoqiangtou mechanism;
Figure 15 is the working state schematic representation of Figure 13 Zhong Tuoqiangtou mechanism;
Wherein: 100, frame; 110, side plate; 120, fixed mount; 121, guide pole; 210, reagent rack; 220, reagent rack; 230, the first guide rail; 240, the second guide rail; 250, driver element; 260, driver element; 261, the second motor; 262, driving pulley; 263, driven pulley; 264, driving belt; 300, conveying assembly; 310, driving chain; 311, chain link; 312, bending sheet; 313, outer brace; 314, interior brace; 315, swivel base; 320, main drive shaft; 321, drive sprocket; 330, driven shaft; 331, driven sprocket; 340, dyed plate; 341, dyeing storehouse; 342, installing plate; 343, pivot; 344, gear; 350, driven unit; 351, the 3rd motor; 352, drive lead screw; 353, feed screw nut; 354, contiguous block; 355, line slideway; 356, tooth bar; 357, web joint; 358, photoelectric sensor; 359, shadow shield; 400, erecting frame; 401, riser; 402, orienting sleeve; 403, crossbeam is installed; 404, sliding sleeve; 405, line slideway; 410, transfer pipet; 411, defining flange; 412, catch; 413, spring; 421, pressing plate; 422, through hole; 423, link; 431, the 4th motor; 432, drive lead screw; 433, feed screw nut; 441, the 5th motor; 442, drive lead screw; 443, feed screw nut; 500, air pump; 60, rifle head.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the present invention is described further:
For cell-stain apparatus of the present invention, it comprises frame 100, multiple dyed plate 340, conveying assembly 300, reagent rack 210, reagent rack 220, erecting frame 400, multiple transfer pipet 410 and the air pump 500 equal with transfer pipet 410 quantity.In figure, X is to the length direction referring to frame 100, and Y-direction refers to the Width of frame 100, and Z-direction refers to the short transverse of frame 100.
Frame 100, as the installation foundation of whole device, comprises the side plate 110 and base that are positioned at its Width both sides, and the bottom of base is provided with the feet for supporting whole device, and the parts that have of cell-stain apparatus are between biside plate 110.
The Width of the bearing of trend frame 100 of dyed plate 340 is consistent, conveying assembly 300 is mainly used for the length direction of multiple dyed plate 340 along frame 100 to move, it comprises main drive shaft 320, driven shaft 330, first motor (not shown) and two driving chains 310, wherein, main drive shaft 320 and driven shaft 330 lay respectively at the two ends of conveying mechanism and parallel to each other, the two ends of main drive shaft 320 respectively key are connected with a drive sprocket 321, the two ends of driven shaft 330 respectively key are connected with a driven sprocket 331, the synchronous respectively drive sprocket 321 and driven sprocket 331 that winding is being positioned at the same side of two driving chains 310 is outside, thus by main drive shaft 320 and the synchronous connection of driven shaft 330.The rotating shaft of the first motor can be with main drive shaft 320 directly key be connected, also can be synchronously connect with belt gear or gear-driven mode and main drive shaft 320.
Driving chain 310 is made up of multiple chain link 311, a connection piece is provided with between two adjacent chain links 311, this web member particularly comprises an outer brace 313 and interior brace 314, and the two ends of its China and foreign countries' brace 313 and interior brace 314 are hinged with the end of two chain links 311 be adjacent respectively.Interior brace 314 upper end is formed with the bending sheet 312 of an inside bending, and the upper surface of bending sheet 312 is fixed with a swivel base 315.The two ends of dyed plate 340 are articulated on two relative swivel bases 315 of two driving chains 310 respectively, particularly, an installing plate 342 is respectively arranged with at the two ends of dyed plate 340, wherein, the end winding support of dyed plate 340 is in the bottom of installing plate 342, the top pars intermedia of two installing plates 342 is all fixed with an outward extending pivot 343, that is, the axis of pivot 343 and dyed plate 340 to stagger certain distance mutually in short transverse, the center line of two pivots 343 overlaps, and these two pivots 343 are installed on two relative swivel bases 315 of two driving chains 310 respectively by bearing, namely installing plate 342 can pivotally 343 axis rotate relative to swivel base 315, namely dyed plate 340 can pivotally 343 axis rotate relative to swivel base 315.
First driven by motor main drive shaft 320 rotates, two driving chains 310 are along with drive sprocket 321 and the synchronous motion of driven sprocket 331, namely two relative on two driving chains 310 swivel bases 315 are synchronized with the movement, that is, the two ends of dyed plate 340 are synchronized with the movement, when dyed plate 340 moves to driving chain 310 turning, owing to there is certain distance between dyed plate 340 and pivot 343, at the turning of driving chain 310, under the effect of dyed plate 340 gravity suffered by it, its upper face remains horizontality, that is, the opening on top, dyeing storehouse 341 fixing on dyed plate 340 all the time upward, thus can ensure that the reactant liquor dyeed in storehouse 341 can not be poured out.Most importantly, in above-mentioned use procedure, owing to adopting driving chain 310 to drive dyed plate 340 to move, make multiple dyed plate 340 along driving chain 310 shuttling movement, therefore effectively can reduce the space that conveying mechanism takies.
It should be noted that, in such scheme, that a bending sheet 312 is set on interior brace 314 for installing swivel base 315, in the present invention, also can be outside brace 313 arranges one for installing the parts of swivel base 315, or the web member connecting two chain links 311 also can adopt other forms, as long as while can meeting pivot 343 pivot joint, and does not hinder the proper motion of driving chain 310.
In the front end of frame 100, a photoelectric sensor is also installed, this photoelectric sensor is a kind of conventional correlation photoelectricity, driving chain 310 is provided with an anti-dazzling screen, this anti-dazzling screen is when moving along with driving chain 310, can through the transmitting terminal of correlation photoelectricity and receiving end, make correlation photoelectricity send signal to a controller, controlled unlatching and the stopping of the first motor by controller, some dyed plates 340 are parked in immediately below erecting frame 400 accurately.
In above scheme, in order to realize the conveying circulated, also can be utilize feed belt to replace driving chain 310, only needing the two ends of dyed plate 340 to be articulated in respectively on the feed belt of both sides.
The present invention also includes a upset liquid pouring machine structure coordinated with conveying assembly 300, it comprises tooth bar 356, driven unit 350 and gear 344, wherein multiple dyed plate 340 is parallel to each other, and along the equally spaced arrangement of extension track of driving chain 310, synchronously on the pivot 343 of dyed plate 340 one end be connected with a gear 344, this gear 344 is for coordinating with tooth bar 356, realize the upset of dyed plate 340, thus make the dyeing storehouse on dyed plate 340 opening down, pour out the liquid in dyeing storehouse, concrete mode is, being arranged on of above-mentioned tooth bar 356 activity is positioned on the side plate 110 of the same side with gear 344, the orientation of the double wedge on this tooth bar 356 is consistent with the length direction of frame 100, driven unit 350 is for moving with the Width of carry-over bar 356 in frame 100, namely driven unit 350 moves in a position and of engaging with gear 344 below gear 344 and gear 344 with carry-over bar 356 between the position that frame 100 Width staggers mutually, when needing when on some dyed plates 340, the liquid dyeed in storehouse 341 is poured out, then be transferred assembly 300 at this dyed plate 340
Drive to being positioned at before the position of tooth bar 356, start driven unit 350, tooth bar 356 is driven above-mentioned position of engaging with gear 344, when dyed plate 340 moves to the position overturning liquid, first gear 344 engages with tooth bar 356 front end, when gear 344 moves along the length direction (i.e. the length direction of tooth bar 356) of frame 100 along with dyed plate 340, gear 344 rotates slowly, thus drives dyed plate 340 to overturn.
Driven unit 350 is comprised body part and is fixed on the 3rd motor 351 on side plate 110 by a web joint 357, the drive lead screw 352 extended in the rotating shaft being synchronously connected in the 3rd motor 351 and along the Width of frame 100, the feed screw nut 353 be socketed on drive lead screw 352 of coupling, be arranged on two line slideways 355 extended on web joint 357 and along frame 100 Width, feed screw nut 353 is fixed with a contiguous block 354, what slided respectively in the two ends of tooth bar 356 is arranged on two line slideways 355, the top ends of contiguous block 354 is fixedly connected with the pars intermedia of tooth bar 356, start the 3rd motor 351, carry-over bar 356 can be with to move along line slideway 355, namely the Width along frame 100 moves.
Conveniently control, improve automaticity of the present invention, web joint 357 is installed with one and sends the photoelectric sensor 358 of signal to controller, contiguous block 354 is provided with a shadow shield 359, this shadow shield 359 moves at the Width of frame 100 along with contiguous block 354 and tooth bar 356, particularly, this shadow shield 359 can move between a position between the transmitting terminal and receiving end of photoelectric sensor 358 and a position be separated with photoelectric sensor 358, when shadow shield 359 is between the transmitting terminal and receiving end of photoelectric sensor 358, the receiving end of photoelectric sensor 358 can not receive the light signal of transmitting terminal transmission, now, photoelectric sensor 358 sends to the signal of controller to be the signal that the 3rd motor 351 is opened, otherwise, when shadow shield 359 is separated with photoelectric sensor 358, then the receiving end of photoelectric sensor 358 can receive the light signal that transmitting terminal sends, now, photoelectric sensor 358 sends to the signal of controller to be the signal that the 3rd motor 351 is stopped.In concrete use procedure, have and need the time of falling liquid, the 3rd motor 351 can be allowed with carry-over bar 356 to move to the position of engaging with gear 344, this just needs above-mentioned controller to be in holding state in normal cell dyeing process, namely, controller connects one and is transformed into the trigger switch of duty for making controller from holding state, when needs overturn the time of falling liquid, open the moment of trigger switch, controller is started working, due to when original state, between the transmitting terminal that shadow shield 359 is in photoelectric sensor 358 and receiving end, then open the moment of trigger switch, controller controls the 3rd motor 351 and opens, when tooth bar 356 is in the state engaged with gear 344, shadow shield 359 is just in time separated with photoelectric sensor 358, controller controls the 3rd motor 351 to be stopped.
Above-mentioned driven unit 350 also can be with carry-over bar 356 to move in the height direction, namely when needs upset dyed plate 340, driven unit 350 is with carry-over bar 356 to move upward and is engaged with gear 344, when not needing upset dyed plate 340, driven unit 350 band carry-over bar 356 moves downward and is separated with gear 344, that is, in the present invention, move between the position that driven unit 350 needs can engage with gear 344 with carry-over bar 356 and a position be separated with gear 344.
The first guide rail 230 and the second guide rail 240 that the length direction along frame 100 extends is separately installed with in the both sides of frame 100, what slided respectively in the both sides of reagent rack 210 and reagent rack 220 is arranged on the first guide rail 230 and the second guide rail 240, driver element 250 is arranged in frame 100 for driving reagent rack 210 along length direction (namely along the length direction of the first guide rail 230 and the second guide rail 240) motion of frame 100, driver element 260 is arranged in frame 100 for driving reagent rack 220 along length direction (namely along the length direction of the first guide rail 230 and the second guide rail 240) motion of frame 100.Driver element 250 driver element 260 structure is identical, for driver element 260, it comprises second motor 261 of body partial fixing in frame 100, be positioned at the rearward end of frame 100 and the driving pulley 262 be synchronously connected in the second motor 261 rotating shaft, be articulated in the driven pulley 263 of frame 100 leading section, the synchronous driving belt 264 being set around driving pulley 262 and driven pulley 263 outside, the side of reagent rack 220 is connected on driving belt 264 by web joint, start the second motor 261, transmission reagent rack 220 can be driven to move along the length direction of frame 100.Because the structure of driver element 250 is identical with driver element 260, do not do repeat specification here, the side of reagent rack 210 is connected on the driving belt of driver element 250 by web joint.
In the present invention, driver element 250 and driver element 260 can also be by the lead screw transmission mechanism etc. of driven by motor, as long as can drive reagent rack 210 and reagent rack 220 sliding along the first guide rail 230 and the second guide rail 240 independently.Driver element 250 and driver element 260 lay respectively at the outside of the first guide rail 230 and the second guide rail 240, thus reagent rack 210 and reagent rack 220 can not be interfered along the to-and-fro movement of frame 100 length direction.Reagent rack 210 can be utilized to place sample hose and the rifle head for sample thief, reagent rack 220 is utilized to place coloring agent and the rifle head for getting coloring agent, reagent rack 210 and reagent rack 220 are independently moved, can not interfere mutually, and share the first guide rail 230 and the second guide rail 240 both it, while facilitating use, simplify the structure of dyeing apparatus.
Multiple transfer pipet 410 of the present invention is arranged on the bottom of erecting frame 400, and the equally spaced width along frame 100 forms a line, wherein, erecting frame 400 is movably arranged on the fixed mount 120 in frame 100, it installation crossbeam 403 comprising riser 401 and be fixed on riser 401 bottom, wherein, the both sides of riser 401 are installed with orienting sleeve 402 respectively, on the guide pole 121 being socketed in fixed mount 120 both sides that the orienting sleeve 402 of both sides slides respectively, guide pole 121 extends along the short transverse of frame 100, namely erecting frame 400 can move up and down along the short transverse of frame 100 relative to fixed mount 120, above-mentioned multiple transfer pipets 410 are installed in be installed on crossbeam 403, and be arranged in row along the length direction installing crossbeam 403, certainly, the plurality of transfer pipet 410 also can be arranged on other arrangement mode to install on crossbeam 403, if can with reagent rack 210 and reagent rack 220 on to put the position, hole of rifle head unanimous between the higher and lower levels.Install on crossbeam 403 and be fixed with multiple sliding sleeve 404 equal with transfer pipet 410 quantity, the axis in the hole of sliding sleeve 404 inside is consistent with short transverse, transfer pipet 410 slip cross-under is one to one in sliding sleeve 404, the top ends of transfer pipet 410 is provided with a catch 412, this catch 412 is positioned at above sliding sleeve 404 top ends, it is for being articulated in sliding sleeve 404 by transfer pipet 410, prevent transfer pipet 410 from moving downward relative to installation crossbeam 403, transfer pipet 410 is avoided to come off from installation crossbeam 403, transfer pipet 410 is also provided with one and is positioned at defining flange 411 below catch 412, transfer pipet 410 outer cover is connected to a spring 413, this spring 413 is limited between the bottom face of defining flange 411 and sliding sleeve 404, pressurized under this spring 413 normality, it can provide elastic stress transfer pipet 410 being pushed up pressure downwards.Moving up and down of erecting frame 400 is driven by one the 4th motor 431, particularly, the body partial fixing of the 4th motor 431 is on fixed mount 120, the rotating shaft of the 4th motor 431 is synchronously connected with a drive lead screw 432 extended along the short transverse of frame 100, on drive lead screw 432, coupling is socketed with a feed screw nut 433, riser 401 is fixedly connected on this feed screw nut 433, start the 4th motor 431, erecting frame 400 can be driven to move up and down along short transverse relative to frame 100, namely drive above-mentioned multiple transfer pipets 410 to move downward to carry out the action of head of taking arms.
Open the 4th motor 431, drive transfer pipet 410 downwardly rifle headstock motion, transfer pipet 410 is inserted by the upper opening of rifle head, after transfer pipet 410 inserts a segment distance, the resistance that rifle head applies transfer pipet 410 is increasing, transfer pipet 410 starts to move upward relative to installation crossbeam 403, simultaneously, the elastic stress that spring 413 is applied on transfer pipet 410 increases gradually, at above-mentioned transfer pipet 410 with the process of rifle head plug-in mounting, due to the buffer action of spring 413, for the rifle head that openings of sizes is different, there is small difference in the distance of transfer pipet 410 actual movement downwards, particularly, for the rifle head that opening is larger, the actual distance moved down of the transfer pipet 410 corresponding with it is larger, and for the less rifle head of opening, the actual distance moved down of the transfer pipet 410 corresponding with it is less, finally, after installation crossbeam 403 moves down certain distance, can ensure that all transfer pipets 410 all coordinate with rifle head is gapless.
It should be noted that, also can without the need to arranging sliding sleeve 404 on installation crossbeam 403 in above-mentioned scheme, can be directly crossbeam 403 offers multiple quantity and bearing of trend equal with transfer pipet 410 and the consistent through hole of short transverse installing, the cross-under of transfer pipet 410 activity is in through hole, in order to prevent transfer pipet 410 there is beat with during rifle head plug-in mounting, the thickness installing crossbeam 403 can be increased, make the through hole on it have greater depth.
In addition, in order to move after liquid completes, realize coming off of rifle head 60, riser 401 is provided with a pressing plate 421, particularly, pressing plate 421 is positioned to be installed immediately below crossbeam 403, and in side direction, bending forms a link 423 thereafter, what link 423 slided be arranged on is positioned on the line slideway 405 extended along frame 100 short transverse at riser 401 back side, and pressing plate 421 line slideway 405 of can postponing slides up and down relative to erecting frame 400;
Pressing plate 421 is provided with multiple through hole 422, the plurality of through hole 422 and transfer pipet 410 one_to_one corresponding, transfer pipet 410 is connected in the through hole 422 corresponding with it, the width of this through hole 422 is slightly larger than the diameter of transfer pipet 410, when moving relative to erecting frame 400 to avoid pressing plate 421, transfer pipet 410 and pressing plate 421 collide, the width of through hole 422 is then slightly less than the diameter of rifle head 60 top ends, that is, rifle head 60 can not be passed by through hole 422, the periphery of through hole 422 forms one for pushing up the top splenium of pressure rifle head 60 top ends, when transfer pipet 410 being equipped with rifle head 60 and move relative to erecting frame 400 when pressing plate 421 downwards, the top splenium of through hole 422 periphery can push up the top end face of pressure rifle head 60 downwards, rifle head 60 is moved downward depart from transfer pipet 410.Moving up and down of pressing plate 421 is driven by the 5th motor 441 of body partial fixing on riser 401, the rotating shaft of the 5th motor 441 is synchronously connected with a drive lead screw 442 extended along frame 100 short transverse, on drive lead screw 442, coupling is socketed with a feed screw nut 443, this feed screw nut 443 is fixedly connected on link 423, start the 5th motor 441, dynamic pressure plate 421 can be with to move up and down relative to erecting frame 400.
When needing the rifle head 60 taken off on transfer pipet 410, open the 5th motor 441, pressing plate 421 is driven to move downward, when pressing plate 421 moves to the position contacted with rifle head 60 top ends, top splenium pressing plate 421 being positioned at through hole 422 periphery starts top pressure rifle head 60 downwards, until rifle head 60 and transfer pipet 410 depart from completely, afterwards, 5th motor 441 rotates backward, and band dynamic pressure plate 421 gets back to initial position, and transfer pipet 410 prepares 60 action of next time taking arms.
The present invention in use, conveying assembly 300 is utilized to drive multiple dyed plate 340 moving along the length direction interval of frame 100, be positioned at reagent rack 210 above conveying assembly 300 and reagent rack 220 is respectively used to place sample hose and coloring agent that cell sample is housed, multiple transfer pipets 410 are positioned at the top of reagent rack 210 and reagent rack 220, when dyed plate 340 is transported to immediately below transfer pipet 410 by conveying assembly 300, dyed plate 340 rests on this position, now reagent rack 210 moves backward to immediately below transfer pipet 410, transfer pipet 410 gets back to elemental height after moving down head of taking arms in reagent rack 210, reagent rack 210 is moving along frame 100 length direction, transfer pipet 410 is made just in time to be positioned at the top of a stock layout QC, now transfer pipet 410 gets back to elemental height after again moving down and drawing cell sample, reagent rack 210 transverse shifting, transfer pipet 410 moves down the multiple dyeing storehouses 341 on the dyed plate 340 that to be injected into by the sample in rifle head and to be positioned at immediately below it, turn down rifle head afterwards, reagent rack 220 moves forward to the below of transfer pipet 410, transfer pipet 410 is taken arms after head in reagent rack 220, coloring agent is drawn again from reagent rack 220, reagent rack 220 moves to initial position backward afterwards, transfer pipet 410 drives rifle head to be injected into by coloring agent to have in the dyeing storehouse of cell sample, after completing the dyeing storehouse fluid injection on a dyed plate 340, conveying assembly 300 drives multiple dyed plate 340 to move, next dyed plate 340 is made to be positioned at below transfer pipet 410, repeat above-mentioned action.In above operating process, suction liquid is realized by air pump 500.Complete when whole dyeing storehouses all completes fluid injection, and after needing to pour out the liquid in dyeing storehouse, then start the 3rd motor 351, tooth bar 356 is moved inward along the Width of frame 100, the gear 344 moved to subsequently on the dyed plate 340 at tooth bar 356 place all can engage with tooth bar 356, and liquid is fallen in the upset realizing dyeing storehouse 341.Above without the need to manually, there is higher automaticity, improve the efficiency of cell dyeing, and the pollution that human contact causes can be avoided.
To one skilled in the art, according to technical scheme described above and design, other various corresponding change and deformation can be made, and all these change and deformation all should belong within the protection domain of the claims in the present invention.
Claims (12)
1. cell-stain apparatus, is characterized in that, comprises,
Frame;
The dyed plate that multiple bearing of trend is consistent with the Width of frame, all dismountablely on each dyed plate is provided with multiple dyeing storehouse, and the plurality of dyeing storehouse arranges along the length direction of dyed plate;
Be arranged on the conveying assembly for driving multiple dyed plate to move at the length direction of frame of frame lower;
Movable to be arranged in frame and two reagent rack be positioned at above conveying assembly, these two reagent rack are respectively used to placement and histiocytic sample hose and coloring agent are housed, and both it on all have for placing the position, hole of getting liquid rifle head;
Drive two driver elements that two reagent rack are moved at the length direction of frame respectively;
Be movably arranged on the erecting frame in frame, the bottom of this erecting frame is provided with multiple transfer pipet along the arrangement of frame Width, and the upper end open of transfer pipet is communicated with rack-mounted air pump by flexible pipe;
For driving erecting frame along the up and down driven unit of the short transverse of frame.
2. cell-stain apparatus as claimed in claim 1, it is characterized in that, conveying assembly comprises,
Be articulated in the main drive shaft of frame length direction one end, main drive shaft extends along the Width of frame, and its two ends are synchronously connected with a drive sprocket respectively;
Be articulated in the driven shaft of the frame length direction other end, driven shaft extends along the Width of frame, and its two ends are synchronously connected with a driven sprocket respectively;
The first motor rotated for driving main drive shaft;
Two driving chains, the drive sprocket of the respectively synchronous winding of this two driving chain in the same side and driven sprocket outside; Driving chain is made up of multiple chain link, a connection piece is provided with between two adjacent chain links, the two ends of web member are hinged with the chain link being positioned at its two ends respectively, and web member is fixed with swivel base, and the two ends of dyed plate are articulated in two swivel bases relative on two driving chains respectively.
3. cell-stain apparatus as claimed in claim 2, it is characterized in that, the two ends of dyed plate are respectively arranged with an installing plate, the end winding support of dyed plate is inside the bottom of installing plate, the top pars intermedia of two installing plates is provided with outward extending pivot, the center line of two pivots overlaps, and two pivots are installed on two swivel bases relative on two driving chains respectively by bearing.
4. cell-stain apparatus as claimed in claim 2, it is characterized in that, the front end of frame is provided with a photoelectric sensor, one of them driving chain is provided with a shadow shield corresponding with this photoelectric sensor.
5. cell-stain apparatus as claimed in claim 1, is characterized in that, the both sides of frame are respectively arranged with a guide rail extended along frame length direction, and what slided respectively in the both sides of two reagent rack is arranged on two guide rails; Driver element comprises second motor of body partial fixing in frame, be articulated in frame length direction one end and the driving pulley that the second motor in synchrony connects, the driven pulley being articulated in the frame length direction other end, the synchronous belt of synchronous winding in driving pulley and driven pulley outside, and two reagent rack are fixedly connected on the synchronous belt of two driver elements respectively.
6. cell-stain apparatus as claimed in claim 1, it is characterized in that, this cell-stain apparatus also includes a upset liquid pouring machine structure, this upset liquid pouring machine structure comprises, be arranged on the gear of dyed plate end, movable to be arranged in frame and the orientation of double wedge on it tooth bar consistent with frame length direction, for carry-over bar one and the position of gears meshing and the driving mechanism that moves between one and the gear position of mutually staggering.
7. cell-stain apparatus as claimed in claim 6, it is characterized in that, driving mechanism comprises three motor of body partial fixing in frame, to be synchronously connected on the 3rd machine shaft and being socketed in the feed screw nut transmitted on screw mandrel, being arranged in frame and bearing of trend and the consistent line slide rail of frame Width of the bearing of trend drive lead screw consistent with the Width of frame, coupling, and tooth bar is fixedly connected on feed screw nut and goes up and being arranged on line slide rail of sliding.
8. cell-stain apparatus as claimed in claim 1, it is characterized in that, driven unit comprises four motor of a body partial fixing in frame, and erecting frame connects with the rotating shaft of the mode of screw rod transmission with the 4th motor.
9. cell-stain apparatus as claimed in claim 8, it is characterized in that, the cross-under of transfer pipet activity also can be movable relative to erecting frame along the short transverse of frame on erecting frame, the top ends of transfer pipet is provided with the catch for preventing transfer pipet from moving downward relative to erecting frame, transfer pipet is provided with the defining flange be positioned at below catch; Defining flange on each transfer pipet and be provided with a spring between erecting frame, the transfer pipet corresponding with it is pushed up the elastic stress of pressure for providing one by this spring up and down.
10. cell-stain apparatus as claimed in claim 9, it is characterized in that, the bottom of erecting frame is fixed with multiple sliding sleeve equal with transfer pipet quantity, transfer pipet activity cross-under is one to one in sliding sleeve, catch is mounted on the top ends of sliding sleeve, and spring is limited between defining flange and the bottom face of sliding sleeve.
11. cell-stain apparatuses as claimed in claim 1, it is characterized in that, the bottom activity of erecting frame a pressing plate is installed, erecting frame is provided with one the 5th motor, the rotating shaft of the 5th motor to be connected with pressing plate in the mode of screw rod transmission and for moving up and down relative to erecting frame in the short transverse of frame with dynamic pressure plate, pressing plate is provided with the through hole passed for transfer pipet, the periphery of through hole is formed with the top splenium pushing up the end, the rifle crown on pressure transfer pipet when pressing plate moves downward relative to erecting frame downwards.
12. cell-stain apparatuses as claimed in claim 11, is characterized in that, the width of through hole is greater than the diameter of transfer pipet, is less than the diameter of rifle cephalic par.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310470832.5A CN103558074B (en) | 2013-10-10 | 2013-10-10 | Cell-stain apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310470832.5A CN103558074B (en) | 2013-10-10 | 2013-10-10 | Cell-stain apparatus |
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| CN103558074A CN103558074A (en) | 2014-02-05 |
| CN103558074B true CN103558074B (en) | 2016-04-13 |
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| CN201310470832.5A Active CN103558074B (en) | 2013-10-10 | 2013-10-10 | Cell-stain apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103897980B (en) * | 2014-02-26 | 2016-02-10 | 聂棱 | A kind of liquid transfer gun head inserting device for biomolecules purification & isolation |
| CN107760561B (en) * | 2017-10-26 | 2019-05-28 | 深圳市汇研科创生物科技有限公司 | Nucleic acid-extracting apparatus and method for extracting nucleic acid |
| CN109297792B (en) * | 2018-10-18 | 2023-12-26 | 广州安必平医药科技股份有限公司 | Slice-making and dyeing integrated machine |
| CN109297794B (en) * | 2018-10-18 | 2023-12-26 | 广州安必平医药科技股份有限公司 | Dyeing plate and slice-making dyeing integrated machine |
| CN114689488B (en) * | 2022-04-21 | 2023-01-17 | 伊尔瑞生物科技(江苏)有限公司 | Lymphocyte counting and detecting micro-fluidic device for cell analysis and method thereof |
| CN116084108B (en) * | 2023-02-16 | 2023-09-19 | 杭州萧山桥南织造有限公司 | Dip dyeing device for dyeing textile fabric and processing technology thereof |
| CN116959168B (en) * | 2023-07-24 | 2024-03-05 | 滁州市精美家电设备股份有限公司 | Unmanned vending machine with LED show cupboard |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2883294B2 (en) * | 1995-07-07 | 1999-04-19 | アロカ株式会社 | Biological tissue processing device and reagent processing unit |
| DE10006084B4 (en) * | 2000-02-11 | 2009-01-02 | Leica Biosystems Nussloch Gmbh | Stainer with a heating station |
| US6998270B2 (en) * | 2001-11-26 | 2006-02-14 | Lab Vision Corporation | Automated tissue staining system and reagent container |
| CN101021455B (en) * | 2007-03-22 | 2010-10-06 | 广州安必平医药科技有限公司 | Slide-making staining machine and method for slide-making and staining |
| US8877485B2 (en) * | 2009-12-09 | 2014-11-04 | Dako Denmark A/S | Apparatus and method for processing biological samples |
| US9028778B2 (en) * | 2011-12-28 | 2015-05-12 | Abbott Laboratories | Accelerated Wright-Giemsa and May-Grünwald staining methods |
| CN102607914B (en) * | 2012-03-08 | 2015-06-17 | 张晓东 | Turnover cell tabletting device |
| CN102759474A (en) * | 2012-06-13 | 2012-10-31 | 长沙康柏恩医疗科技有限公司 | Liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting |
| CN203606196U (en) * | 2013-10-10 | 2014-05-21 | 广州安必平自动化检测设备有限公司 | Cell-staining device |
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Address after: 510665 Room 301, block B, building 2, Kexin street, Huangpu District, Guangzhou, Guangdong Patentee after: GUANGZHOU LBP AUTOMATED TESTING EQUIPMENT Co.,Ltd. Address before: 510665 room 301-1, building B, No. 10 yayingshi Road, Guangzhou high tech Industrial Development Zone, Guangzhou, Guangdong Patentee before: GUANGZHOU LBP AUTOMATED TESTING EQUIPMENT Co.,Ltd. |