CN107760561B - Nucleic acid-extracting apparatus and method for extracting nucleic acid - Google Patents
Nucleic acid-extracting apparatus and method for extracting nucleic acid Download PDFInfo
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- CN107760561B CN107760561B CN201711012247.5A CN201711012247A CN107760561B CN 107760561 B CN107760561 B CN 107760561B CN 201711012247 A CN201711012247 A CN 201711012247A CN 107760561 B CN107760561 B CN 107760561B
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- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The present invention provides a kind of nucleic acid-extracting apparatus and method for extracting nucleic acid, the nucleic acid-extracting apparatus includes rack, dropping liquid feed mechanism and Beads enrichment mechanism, it further include the first driving group being connect with dropping liquid feed mechanism, the second driving group being connect with dropping liquid feed mechanism and the third driving group being connect with Beads enrichment mechanism, the first driving group, the second driving group and third driving group are connect with the rack.Nucleic acid-extracting apparatus provided by the invention is avoided artificial mobile reagent box, improves the working efficiency of the nucleic acid-extracting apparatus by the first driving group of setting, the second driving group and third driving group so that dropping liquid feed mechanism and Beads enrichment mechanism are removable.
Description
Technical field
The invention belongs to technical field of medical equipment, are to be related to a kind of nucleic acid-extracting apparatus and nucleic acid mentions more specifically
Take method.
Background technique
With the rapid development of medical field, there are more and more biological experiments based on nucleic acid, by right
Extraction, the detection of nucleic acid can make quick diagnosis to the disease of patient.In the device for extracting nucleic acid at present, for distributing reagent
Dropping liquid feed mechanism and Beads enrichment mechanism for extracting nucleic acid it is mutually indepedent, need manually after distributing reagent by kit
It is transferred in Beads enrichment mechanism, causes the working efficiency for extracting nucleic acid lower.
Summary of the invention
The purpose of the present invention is to provide a kind of nucleic acid-extracting apparatus and method for extracting nucleic acid, to solve to deposit in the prior art
Dropping liquid feed mechanism and Beads enrichment mechanism lead to nucleic acid extraction low efficiency independently of each other the problem of.
To achieve the above object, the technical solution adopted by the present invention is that: a kind of nucleic acid-extracting apparatus is provided, including rack,
Dropping liquid feed mechanism and Beads enrichment mechanism, the nucleic acid-extracting apparatus further include connecting and being used for the dropping liquid feed mechanism
The first driving group for driving it to move on first level direction is connect and for driving it the with the dropping liquid feed mechanism
It the second driving group for being moved in two horizontal directions and is connect with the Beads enrichment mechanism and for driving it in third level
The third driving group moved on direction, first driving group, second driving group and the third driving group with it is described
Rack is fixedly connected.
Further, the first level direction and second horizontal direction are mutually perpendicular to, the first level direction
With the third horizontal direction parallel.
Further, the dripping system includes multiple dropping liquid feed units, and the dropping liquid feed unit includes that dropping liquid drives
Dynamic component and the dropping liquid component for containing liquid, multiple dropping liquid components are in array distribution in the dropping liquid driving assembly
Above and with it is separately connected.
Further, the dropping liquid feed unit further includes the limit group for limiting the feed motion of the dropping liquid component
Part, the limit assembly are separately connected with the dropping liquid driving assembly and the dropping liquid component.
Further, the limit assembly includes the first sliding block, elastic component and the institute connecting with the dropping liquid driving assembly
State the second sliding block that the first sliding block is slidably connected, and the limit switch for limiting the elastic component decrement, the limit
Switch is fixedly connected with first sliding block, and the both ends of the elastic component are solid with first sliding block and second sliding block respectively
Fixed connection, the dropping liquid component are fixedly connected with second sliding block.
Further, the Beads enrichment mechanism includes the kit for accommodating magnetic bead, can protrude into the kit and can
The bar magnet component of the magnetic bead is adsorbed, and is connect with the bar magnet component and is used to that it to be driven to protrude into the bar magnet of the kit
Driving assembly.
Further, the Beads enrichment mechanism further includes being sheathed on the bar magnet component and can protruding into the kit
In bar magnet set, adsorbable bar magnet set magnetic sheet and connect with the magnetic sheet and the magnetic for driving the magnetic sheet to move
Plate driving assembly, the bar magnet set is every set between the bar magnet component and the kit.
Further, the magnetic sheet is equipped with the first magnetic pole, and the bar magnet, which is put on, to be equipped with and can adsorb with first magnetic pole
The first Attraction block.
Further, it is additionally provided with the second magnetic pole for adsorbing the kit on the magnetic sheet, is set on the kit
There is the second Attraction block that can be adsorbed with second magnetic pole.
The present invention also provides a kind of method for extracting nucleic acid of nucleic acid-extracting apparatus, comprising the following steps:
Dropping liquid feed mechanism: being moved to the top of the first kit by the first driving group and the second driving group by S10, will
Reagent in dropping liquid feed mechanism is added in kit；
S20: dropping liquid feed mechanism is moved to the top of the second kit by the first driving group and the second driving group；
S30: Beads enrichment mechanism is moved to the top of the first kit by third driving group；
Beads enrichment mechanism: being displaced downwardly in the first kit by S40 by bar magnet driving assembly and magnetic sheet driving assembly, and
Magnetic bead is adsorbed in Beads enrichment mechanism；
S50: Beads enrichment mechanism is moved to the top of the second kit by third driving group.
The beneficial effect of nucleic acid-extracting apparatus provided by the invention is: compared with prior art, nucleic acid extraction of the present invention
Dropping liquid feed mechanism can be moved to the top of kit by the first driving group of setting and the second driving group by device, convenient for that will try
Agent is added in kit；The top that Beads enrichment mechanism can be moved to kit by the way that third driving group is arranged simultaneously, just
In magnetic bead is separated from reagent.In this way, dropping liquid feed mechanism and Beads enrichment mechanism can be moved respectively by multiple driving groups
To the top of kit, artificial mobile reagent box is avoided, the working efficiency of nucleic acid extraction is improved.
Detailed description of the invention
It to describe the technical solutions in the embodiments of the present invention more clearly, below will be to embodiment or description of the prior art
Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only of the invention some
Embodiment for those of ordinary skill in the art without any creative labor, can also be according to these
Attached drawing obtains other attached drawings.
Fig. 1 is the structure chart of nucleic acid-extracting apparatus provided in an embodiment of the present invention；
Fig. 2 is the structure chart 1 of dropping liquid feed mechanism used by the embodiment of the present invention；
Fig. 3 is the structure chart 2 of dropping liquid feed mechanism used by the embodiment of the present invention；
Fig. 4 is the installation diagram of limit assembly used by the embodiment of the present invention and inlet tube；
Fig. 5 is the structure chart of limit assembly used by the embodiment of the present invention (the second sliding block is not shown)；
Fig. 6 is the structure chart of dropping liquid component used by the embodiment of the present invention；
Fig. 7 is the structure chart of Beads enrichment mechanism used by the embodiment of the present invention；
Fig. 8 is the structure chart of magnetic sheet used by the embodiment of the present invention；
Fig. 9 is the structure chart of bar magnet used by the embodiment of the present invention set；
Figure 10 is the structure chart of kit used by the embodiment of the present invention；
Figure 11 is the structure chart of bar magnet component used by the embodiment of the present invention.
Wherein, each appended drawing reference in figure:
1- dropping liquid feed mechanism；10- dropping liquid feed unit；11- dropping liquid driving assembly；111- dropping liquid driving portion；112- drop
Liquid transmission component；1121- driving wheel group；The first belt pulley of 1122-；The second belt pulley of 1123-；1124- belt；12- dropping liquid group
Part；121- inlet tube；122- water dropper；13- limit assembly；The first sliding block of 131-；1311- sliding panel；1312- slider bar；132-
Second sliding block；1321- accommodating cavity；1322- through-hole；1323- light shielding part；133- limit switch；1331- circuit board；1332- sense
Light part；The photosensitive slot of 13320-；134- elastic component；The second guide rail of 135-；The first guide rail of 14-；15- casing；151- casing；2- magnetic bead
Separating mechanism；21- bar magnet driving assembly；22- bar magnet component；221- connecting plate；222- bar magnet；23- magnetic sheet driving assembly；
24- magnetic sheet；241- plate body；The second magnetic pole of 242-；The first magnetic pole of 243-；25- bar magnet set；251- lid；2511- first is adsorbed
Block；2512- lug boss；252- body；26- kit；260- solution cavity；The second Attraction block of 261-；3- rack；4- first drives
Dynamic group；The second driving group of 5-；6 third driving groups.
Specific embodiment
In order to which technical problems, technical solutions and advantages to be solved are more clearly understood, tie below
Accompanying drawings and embodiments are closed, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only
To explain the present invention, it is not intended to limit the present invention.
It should be noted that it can be directly another when element is referred to as " being fixed on " or " being set to " another element
On one element or indirectly on another element.When an element is known as " being connected to " another element, it can
To be directly to another element or be indirectly connected on another element.
It is to be appreciated that term " length ", " width ", "upper", "lower", "front", "rear", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "top", "bottom" "inner", "outside" is that orientation based on the figure or position are closed
System, is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must have
Specific orientation is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more of the features.In the description of the present invention, the meaning of " plurality " is two or more,
Unless otherwise specifically defined.
Referring to Fig. 1, existing be illustrated nucleic acid-extracting apparatus provided by the invention.The nucleic acid-extracting apparatus, including
Rack 3, dropping liquid feed mechanism 1, Beads enrichment mechanism 2, the first driving group 4, the second driving group 5 and third driving group 6.Dropping liquid into
It is connected to 1 second driving group 5 of mechanism, the second driving group 5 and the connection of the first driving group 4, the first driving group 4 drive dropping liquid to feed machine
Structure 1 moves on first level direction, and the second driving group 4 driving dropping liquid feed mechanism 1 moves in the second horizontal direction, the
Make dropping liquid feed mechanism 1 can be close to or far from kit 26 under the drive of one driving group 4 and the second driving group 5；Third driving group
6 connect with Beads enrichment mechanism 2, and third driving group 6 drives Beads enrichment mechanism 2 to move on first level direction, make magnetic bead
Separating mechanism 2 can be close to or far from kit 26.
Nucleic acid-extracting apparatus provided by the invention, compared with prior art, nucleic acid-extracting apparatus of the present invention pass through setting the
Dropping liquid feed mechanism 1 can be moved to the top of kit 26 by one driving group 4 and the second driving group 5, convenient for reagent to be added to
In kit 26；The top that Beads enrichment mechanism 2 can be moved to kit 26 by the way that third driving group 6 is arranged simultaneously, is convenient for
Magnetic bead is separated from reagent.In this way, the nucleic acid-extracting apparatus can pass through the mobile dropping liquid feed mechanism 1 of multiple driving groups and magnetic bead
Separating mechanism 2 avoids artificial mobile reagent box 26, improves the working efficiency of nucleic acid extraction.
Further, referring to Fig. 1, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, institute
It states first level direction and second horizontal direction is mutually perpendicular to, the first level direction and the third horizontal direction are flat
Row.Specifically, first level direction is the length direction of rack 3, and the second horizontal direction is the width direction of rack 3.
Further, please refer to figs. 2 and 3, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid feed mechanism 1 include multiple dropping liquid feed units 10, and dropping liquid feed unit 10 includes dropping liquid driving assembly 11 and dropping liquid
Component 12, dropping liquid driving assembly 11 include providing the dropping liquid transmission component 112 of the dropping liquid driving portion 111 and transmitting power of power,
Dropping liquid component 12 squeezes the liquid in dropping liquid component 12 for containing liquid, and by devices such as external syringes or air pump
Out.Dropping liquid driving assembly 11 is connect with dropping liquid component 12, and specifically, dropping liquid transmission component 112 is fixedly connected with dropping liquid component 12.
Dropping liquid driving portion 111 and dropping liquid transmission component 112 in single dropping liquid feed unit 10 can control single dropping liquid component 12, institute
It can be controlled separately with each dropping liquid component 12 in the dropping liquid feed mechanism 1, promote its performance.
Further, please refer to figs. 2 and 3, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid transmission component 112 include the driving wheel group 1121 connecting with dropping liquid driving portion 111, the first belt pulley 1122, the second skin
Belt wheel 1123 and belt 1124, belt 1124 are set around driving wheel group 1121, the first belt pulley 1122 and the second belt pulley
On 1123, and belt 1124 is fixedly connected with dropping liquid component 12.Specifically, dropping liquid driving portion 111 can be motor, the output of motor
Axis is fixedly connected with driving wheel group 1121.
Further, please refer to figs. 2 and 3, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
The outer diameter of formula, the first belt pulley 1122 and the second belt pulley 1123 is equal, then the first belt pulley 1122 and the second belt pulley 1123
Transmission ratio be 1:1, be driven it is more stable.The axial connecting line of first belt pulley 1122 and the second belt pulley 1123 and dropping liquid component
12 direction of feed is parallel to each other, and the direction of feed of dropping liquid component 12 is vertical direction, so the first belt pulley 1122 and second
The axial connecting line direction of belt pulley 1123 is also vertical direction.Belt 1124 is fixedly connected with dropping liquid component 12, so dropping liquid group
For part 12 when the axial connecting line direction of the first belt pulley 1122 and the second belt pulley 1123 is vertical direction, feed motion is more
Steadily.Certainly, the outer diameter of the first belt pulley 1122 and the second belt pulley 1123 can also be unequal, according to actually required transmission ratio
The outer diameter of first belt pulley 1122 and the second belt pulley 1123 is set.
Further, please see Fig. 2 to Fig. 4, as a kind of specific embodiment party of nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid feed unit 10 further include limit assembly 13, and limit assembly 13 is for limiting dropping liquid component 12 in its direction of feed
Movement.Limit assembly 13 includes the first sliding block 131, elastic component 134, the second sliding block 132 and presses for limiting elastic component 134
The limit switch 133 of contracting amount, the first sliding block 131 are fixedly connected with dropping liquid driving assembly 11, specially the first sliding block 131 and drop
Liquid driving portion 111 is fixedly connected, and the second sliding block 132 is slidably connected with the first sliding block 131, and the both ends of elastic component 134 are respectively with
One sliding block 131 is fixedly connected with the second sliding block 132.Dropping liquid component 12 is fixedly connected with the second sliding block 132.When dropping liquid component 12
When downlink encounters barrier, elastic component 134 compresses, and elastic component 134 triggers limit switch 133, limit after reaching certain decrement
Bit switch 133 issues the downlink that electric signal terminates the dropping liquid component 12, and the dropping liquid feed unit 10 is avoided to be squeezed simultaneously by barrier
It is damaged to.
Further, Fig. 4 and Fig. 5 is please referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, limit switch 133 are optoelectronic switch.Optoelectronic switch is to connect circuit using detected material blocking or reflect to light beam
The presence or absence of detection object.Specifically, it in the dropping liquid feed unit 10, when optoelectronic switch is blocked by the second sliding block 132, then drips
Barrier is encountered when liquid 12 downlink of component；When optoelectronic switch is not blocked by the second sliding block 132, dropping liquid component 12 then keeps normal
Feed motion.The response time of optoelectronic switch is short, high resolution and the material less-restrictive to object to be detected, can detect modeling
Object made of the materials such as material, timber, metal.
Further, a kind of specific reality please continue to refer to Fig. 4 and Fig. 5, as nucleic acid-extracting apparatus provided by the invention
Mode is applied, limit switch 133 is equipped with the photosensitive slot 13320 for receiving light, and correspondingly, the second sliding block 132 is equipped with can
The light shielding part 1323 moved in photosensitive slot 13320.Specifically, limit switch 133 includes being fixedly connected with the first sliding block 131
Circuit board 1331 and with the sensitive piece 1332 connected vertically of circuit board 1331, photosensitive slot 13320 is opened on sensitive piece 1332,
Photosensitive slot 13320 is through slot.Light shielding part 1323 is convexly equipped on the second sliding block 132, and when dropping liquid component 12 encounters barrier, second is sliding
Block 132 is moved up relative to the first sliding block 131, and elastic component 134 is compressed, while light shielding part 1323 is also moved up to sense
In light slot 13320, the inner wall of photosensitive slot 13320 is blocked, shielding signals are transmitted to dropping liquid driving portion 111 by circuit board 1331,
Dropping liquid driving portion 111 is set to stop working, dropping liquid component 12 does not continue to downlink.
Further, please refer to figs. 2 and 3, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, dropping liquid feed unit 10 further include casing 15 and the first guide rail 14, and the first guide rail 14 is fixed on the both ends of casing 15, and dropping liquid drives
The side of casing 15 is fixed in dynamic portion 111.First guide rail 14 is slidably connected with the first sliding block 131.First sliding block 131 and dropping liquid group
Up and down motion of the part 12 under the drive of dropping liquid transmission component 112 makes it move up and down more flat under the support of the first guide rail 14
Surely, the accuracy of its stroke when carrying out feed motion of dropping liquid component 12 is improved.
Further, referring to Fig. 5, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, limit
Hyte part 13 further includes the second guide rail 135, and the second guide rail 135 is fixed on the first sliding block 131, and the second sliding block 132 and second is led
Rail 135 is slidably connected, i.e. the second sliding block 132 is slidably connected by the second guide rail 135 with the first sliding block 131.So in dropping liquid group
When part 12 encounters barrier, dropping liquid component 12 moves the second sliding block 132 on the first sliding block 131 and triggers limit switch 133,
To make dropping liquid component 12 stop downlink.Certainly, the first sliding block 131 and being slidably connected for the second sliding block 132 can also pass through it
He realizes mode, is not construed as limiting herein.
Further, Fig. 4 and Fig. 5 is please referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, the first sliding block 131 include the sliding panel 1311 and slider bar 1312 being mutually permanently connected, sliding panel 1311 and the first guide rail 14
Length direction it is vertical, slider bar 1312 is arranged along the length direction of the first guide rail 14, and sliding panel 1311 and slider bar 1312 are equal
It is slidably connected with the first guide rail 14.Specifically, sliding panel 1311 is fixedly connected with limit switch 133, slider bar 1312 and second
Sliding block 132 is slidably connected, and the first sliding block 131 and limit switch 133 are set to the top of the second sliding block 132, and the two of elastic component 134
End is individually fixed on sliding panel 1311 and the second sliding block 132, and elastic component 134 is vertically arranged.It is preferred that elastic component 134 is spring.
Certain first sliding block 131 and limit switch 133 can also be set to the left and right sides or front and rear sides of the second sliding block, can be according to specific
Situation selects the position of the first sliding block 131 and limit switch 133.
Further, referring to Fig. 6, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, drop
Liquid component 12 includes inlet tube 121 and water dropper 122.One end of inlet tube 121 is fixedly connected with the second sliding block 132, sliding second
It moves up and down under the drive of block 132, the other end of inlet tube 121 is connect with water dropper 122, and inlet tube 122 is with inlet tube 121 can
Dismantling connection is fixedly connected.It prevents dropping liquid component 12 from meeting other barriers when moving up and down, avoids influencing inlet tube 121
Feed liquor.Inlet tube 121 is fixed on the second sliding block 132, is moved with the movement of the second sliding block 132.Specifically, casing 15
Lower end be additionally provided with the casing 151 worn for inlet tube 121, and the supporting block being fixedly connected with the first guide rail 14 is (in figure not
Show), after supporting block is fixedly connected with casing 15, and casing 151 is fixedly connected with supporting block, and supporting block is set to casing 151
Side, casing 151 is for protecting inlet tube 121.
Further, Fig. 4 to Fig. 5 is please referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, the through-hole 1322 that the second sliding block 132 is equipped with the accommodating cavity 1321 of a side opening and wears for inlet tube 123, accommodating cavity
1321 and through-hole 1322 be interconnected, and inlet tube 123 protrudes into accommodating cavity 1321.Specifically, the liquid feeding end of inlet tube 123 is stretched
Enter in accommodating cavity 1321, liquid feeding end can external air pump or syringe, the liquid in inlet tube 123 is squeezed into kit.
Open up accommodating cavity 1321 on second sliding block 132 and facilitate other the external feeding devices of inlet tube 123, make feeding device and dropping liquid into
It is separated into mutually independent device to unit 10, mutual movement is unaffected.
Further, referring to Fig. 7, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Pearl separating mechanism 2, including kit 26, bar magnet component 22 and bar magnet driving assembly 21: containing magnetic bead in kit 26, magnetic bead is
Superparamagnetism silica nanometer magnetic bead can combine on micro interface with nucleic acid molecules specific recognition and efficiently, kit 26
On offer solution cavity 260 for accommodating sample and magnetic bead；Bar magnet component 22 can protrude into kit 26 and fill sample and magnetic bead
Solution cavity 260 in, bar magnet component 22 have magnetism, magnetic bead can be adsorbed, so that magnetic bead be separated from sample；Bar magnet driving
Component 21 can drive bar magnet component 22 toward renaturation to protrude into or protrude into kit 26 when separating magnetic bead far from kit 26,
When bar magnet component connection magnetic bead is taken out, far from kit 26.The Beads enrichment mechanism 2 further includes that bar magnet covers 25,24 and of magnetic sheet
Magnetic sheet driving assembly 23: bar magnet set 25 is sheathed on bar magnet component 22, and bar magnet set 25 is every set on bar magnet component 22 and kit 26
Between, when separating magnetic bead, bar magnet set 25 and bar magnet component 22 are protruded into kit 26, and bar magnet component 22 is across bar magnet set 25
Magnetic bead is adsorbed on the surface of bar magnet set 25；The adsorbable bar magnet of magnetic sheet 24 set 25, convenient for independent adsorption magnetic bar set 25, thus just
In the bar magnet set 25 for shifting magnetic bead and more renewing；Magnetic sheet driving assembly 23 can drive magnetic sheet 24 close to or far from bar magnet set 25, just
25 are covered in adsorbing and loosening bar magnet.
When using the nucleic acid-extracting apparatus, dropping liquid feed mechanism 1 is moved by the first driving group 4 and the second driving group 5
It moves to the top of kit 26, dropping liquid component 12 moves down and instills sample in kit 26, and dropping liquid feed mechanism 1 is moved to down
The top of one kit 26；The Beads enrichment mechanism 2 with bar magnet set 25 is moved to by the reagent by third driving group 6 again
The top of box 26, bar magnet set 25 are moved downward to the inside of kit 26, and magnetic bead is adsorbed in the outer wall of bar magnet set 25, Beads enrichment
Mechanism 2 is moved to elsewhere, carries out subsequent experimental operation to magnetic bead；After abandoning bar magnet set 25, Beads enrichment mechanism 2 is adsorbable
New bar magnet set 25.Above step is repeated, can quickly handle large batch of sample, and without cleaning bar magnet component 22.
Further, Fig. 8 and Fig. 9 is please referred to, a kind of specific embodiment party as nucleic acid-extracting apparatus provided by the invention
Formula, magnetic sheet 24 are equipped with the first magnetic pole 243, and bar magnet set 25 is equipped with the first Attraction block 2511, and the first magnetic pole 243 and first is inhaled
It can mutually adsorb for attached piece 2511, i.e. magnetic sheet 24 and bar magnet set 25 can be adsorbed mutually.Under the drive of magnetic sheet driving assembly 23, magnetic
Stick set 25 can protrude into kit 26, so that magnetic bead be made to be adsorbed in the surface that bar magnet covers 25；Under the drive of magnetic sheet driving assembly 23,
Bar magnet set 25 can also be removed from kit 26, convenient for shifting the magnetic bead being adsorbed on 25 surfaces of bar magnet set, and magnetic bead be turned
It moves in kit used in subsequent step.
Further, Fig. 8 and Figure 10 is please referred to, a kind of specific implementation as nucleic acid-extracting apparatus provided by the invention
Mode, the second magnetic pole 242 is additionally provided on magnetic sheet 24, and kit 26 is equipped with the second Attraction block 261, the second magnetic pole 242 and second
Attraction block 261 can be adsorbed mutually, i.e. magnetic sheet 24 and kit 26 can be adsorbed mutually.Under the drive of magnetic sheet driving assembly 23, the
Two magnetic poles 242 can move back and forth on the direction toward and away from kit 26, when the second magnetic pole 242 is close to the second Attraction block
When 261, the second magnetic pole 242 is powered, kit 26 can be picked up, is convenient for transfering reagent box 26, consequently facilitating carrying out subsequent reality
It tests.First magnetic pole 243 and the second magnetic pole 242 are simultaneously operable, so that magnetic sheet 24 can simultaneously inhale bar magnet set 25 and kit 26
It rises, convenient for bar magnet set 25 and kit 26 to be transferred to elsewhere simultaneously.
Further, referring to Fig. 7, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Rod assembly 22 is that permanent magnet, the first magnetic pole 243 and the second magnetic pole 242 are electromagnet.Specifically, bar magnet component 22 protrudes into bar magnet
After set 25, bar magnet component 22 and bar magnet set 25 are protruded into together in kit 26, and bar magnet component 22 has magnetic and mutual with magnetic bead
Attract, and then magnetic bead is separated from sample, when bar magnet component 22 is far from bar magnet set 25, magnetic bead can be covered on 25 from bar magnet and be taken off
It falls；First magnetic pole 243 and the first Attraction block 2511 are close to each other or when abutting against, and the first magnetic pole 243 is powered, the first magnetic pole
243 and bar magnet set 25 attract each other, bar magnet set 25 can follow moving up and down and moving for magnetic sheet 24, convenient for that will be adsorbed with magnetic bead
Bar magnet set 25 takes out, and bar magnet set 25 can be put down after the power-off of the first magnetic pole 243；Second magnetic pole 242 and the second Attraction block 261 are mutually
It is close or when abutting against, the second magnetic pole 242 is powered, the second magnetic pole 242 and kit 26 attract each other, and kit 26 can follow
Magnetic sheet 24 is moved up and down and is moved, kit 26 easy to remove, can put down kit 26 after the power-off of the second magnetic pole 242.
Further, referring to Fig. 8, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Plate 24 includes plate body 241, and bar magnet component 22 is arranged on plate body 241, and multiple holes passed through for bar magnet component 22 are offered on plate,
Plate body 241 is connected with magnetic sheet driving assembly 23, and magnetic sheet 24 moves up and down under the drive of driving assembly, 243 He of the first magnetic pole
Second magnetic pole 242 is fixedly connected on plate body 241.Specifically, the first magnetic pole 243 and the second magnetic pole 242 are set to plate body 241
It is ipsilateral.The quantity of first magnetic pole 243 and the second magnetic pole 242 can be to be multiple, and preferably the first magnetic pole 243 and the second magnetic pole 242 are all provided with
Four corners in 241 side of plate body.
Further, referring to Fig. 9, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, magnetic
Stick set 25 includes lid 251 and multiple bodies 252, and lid 251 and kit 26 are parallel to each other, can be placed on kit 26, keep away
The sample exempted from kit 26 is contaminated during the experiment, and the first Attraction block 2511 is set on lid 251.Multiple bodies
252 arrays can protrude into kit 26 in the side of lid 251, body 252 under the drive of magnetic sheet 24, and move up and down and make
Nucleic acid separation.The quantity of body 252, kit 26 accommodate the solution cavity 260 of sample quantity and bar magnet 222 quantity it is equal,
The quantity of above-mentioned all parts determines according to actual conditions, is not construed as limiting herein.
Further, referring to Fig. 9, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, set
Body 252 has bar magnet chamber, and a side opening of bar magnet chamber protrudes into bar magnet component 22 in body 252 from the opening.In separation magnetic bead
When, each bar magnet 222 of bar magnet component 22 is located at that bar magnet is intracavitary, and bar magnet chamber is located in kit 26, and magnetic bead is attracted to body
252 outside, the outside of sample contamination and body 252, without being infected with bar magnet component 22, in the magnetic for separating next sample
When pearl, bar magnet set 25 can be directly replaced, without cleaning bar magnet component 22, substantially increases the separation effect of the Beads enrichment mechanism 2
Rate.
Further, Figure 11 is please referred to, as a kind of specific embodiment of nucleic acid-extracting apparatus provided by the invention,
Bar magnet component 22 includes connecting plate 221 and multiple bar magnets 222.Connecting plate 221 and bar magnet driving assembly 21 connect, and drive in bar magnet
It is moved up and down under the drive of component 21, protrudes into bar magnet 222 or far from kit 26.Multiple 222 arrays of bar magnet are in connecting plate 221
Side, and can be protruded under the drive of connecting plate 221 in bar magnet chamber.
Further, referring to Fig. 9, a kind of specific embodiment as nucleic acid-extracting apparatus provided by the invention, lid
Body 251 is equipped with the lug boss 2512 that can be abutted against with kit 26.The quantity of lug boss 2512 can at least be set to be multiple
The opposite sides of lid 251 makes have spacing between lid 251 and kit 26.It protrudes into kit 26 and divides in body 252
After magnetic bead, bar magnet need to be covered 25 and be taken out from kit 26, the setting of lug boss 2512 is so that bar magnet covers 25 251 He of lid
Kit 26 will not under the action of atmospheric pressure stick to each other, convenient for take out bar magnet set 25.
The present invention also provides a kind of method for extracting nucleic acid of nucleic acid-extracting apparatus, comprising the following steps:
S10: dropping liquid feed mechanism 1 is moved to by the upper of the first kit 26 by the first driving group 4 and the second driving group 5
Reagent in dropping liquid feed mechanism 1 is added in kit 26 for side；
S20: dropping liquid feed mechanism 1 is moved to by the upper of the second kit 26 by the first driving group 4 and the second driving group 5
Side；
S30: Beads enrichment mechanism 2 is moved to the top of the first kit 26 by third driving group 6；
S40: Beads enrichment mechanism 2 is displaced downwardly to by the first kit by bar magnet driving assembly 21 and magnetic sheet driving assembly 23
In 26, and magnetic bead is adsorbed in Beads enrichment mechanism 2；
S50: Beads enrichment mechanism 2 is moved to the top of the second kit 26 by third driving group 6.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (6)
1. nucleic acid-extracting apparatus, it is characterised in that: including rack, dropping liquid feed mechanism and Beads enrichment mechanism, the nucleic acid is mentioned
Taking device further includes the first driving for connecting with the dropping liquid feed mechanism and being used to that it to be driven to move on first level direction
Group, the second driving group for being connect with the dropping liquid feed mechanism and being used to that it to be driven to move in the second horizontal direction, Yi Jiyu
The Beads enrichment mechanism connection and the third driving group for driving it to move in third horizontal direction, first driving
Group, second driving group and the third driving group are connect with the rack；The Beads enrichment mechanism includes accommodating magnetic
The kit of pearl can protrude into the kit and the bar magnet component of the adsorbable magnetic bead, and connect with the bar magnet component
The bar magnet driving assembly for connecing and being used to that it to be driven to protrude into the kit；The Beads enrichment mechanism further includes being sheathed on the magnetic
On rod assembly and the bar magnet set in the kit can be protruded into, the magnetic sheet that the adsorbable bar magnet covers, and connected with the magnetic sheet
It connects and the magnetic sheet driving assembly for driving it to move, the bar magnet component is arranged in the plate body of the magnetic sheet, the bar magnet
Set is every being set between the bar magnet component and the kit, and the magnetic sheet is equipped with the first magnetic pole, and the bar magnet, which is put on, to be equipped with
The first Attraction block that can be adsorbed with first magnetic pole, the bar magnet set includes lid and multiple bodies, multiple body battle arrays
It is listed in the side of the lid, first Attraction block is set on the lid, and the second magnetic pole is additionally provided on the magnetic sheet, described
Kit is equipped with the second Attraction block that can be adsorbed with second magnetic pole, and first magnetic pole and the second magnetic pole are electromagnetism
Iron.
2. nucleic acid-extracting apparatus as described in claim 1, it is characterised in that: the first level direction and second level
Direction is mutually perpendicular to, the first level direction and the third horizontal direction parallel.
3. nucleic acid-extracting apparatus as described in claim 1, it is characterised in that: the dropping liquid feed mechanism include multiple dropping liquids into
To unit, the dropping liquid feed unit includes dropping liquid driving assembly and the dropping liquid component for containing liquid, multiple drops
Liquid component is separately connected in array distribution on the dropping liquid driving assembly and with it.
4. nucleic acid-extracting apparatus as claimed in claim 3, it is characterised in that: the dropping liquid feed unit further includes for limiting
The limit assembly of the feed motion of the dropping liquid component, the limit assembly and the dropping liquid driving assembly and the dropping liquid component
It is separately connected.
5. nucleic acid-extracting apparatus as claimed in claim 4, it is characterised in that: the limit assembly includes driving with the dropping liquid
First sliding block of component connection, elastic component, the second sliding block being slidably connected with first sliding block, and for limiting the bullet
Property part decrement limit switch, the limit switch is fixedly connected with first sliding block, the both ends difference of the elastic component
It is fixedly connected with first sliding block and second sliding block, the dropping liquid component is fixedly connected with second sliding block.
6. the method for extracting nucleic acid of nucleic acid-extracting apparatus as described in any one in claim 1-5, which is characterized in that including following
Step:
S10: dropping liquid feed mechanism is moved to the top of the first kit by the first driving group and the second driving group, by dropping liquid
Reagent in feed mechanism is added in kit；
S20: dropping liquid feed mechanism is moved to the top of the second kit by the first driving group and the second driving group；
S30: Beads enrichment mechanism is moved to the top of the first kit by third driving group；
Beads enrichment mechanism: being displaced downwardly in the first kit by S40 by bar magnet driving assembly and magnetic sheet driving assembly, and by magnetic
Pearl is adsorbed in Beads enrichment mechanism；
S50: Beads enrichment mechanism is moved to the top of the second kit by third driving group.
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| CN108277148B (en) * | 2018-04-25 | 2024-05-28 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid separation device |
| CN108410859A (en) * | 2018-04-28 | 2018-08-17 | 绍兴迅敏康生物科技有限公司 | Method for extracting nucleic acid |
| CN108774619B (en) * | 2018-06-26 | 2021-09-07 | 杭州优思达生物技术有限公司 | Non-alcoholization nucleic acid detection reagent tube |
| CN111218398B (en) * | 2020-03-25 | 2024-02-13 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
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| CN103558074A (en) * | 2013-10-10 | 2014-02-05 | 广州安必平自动化检测设备有限公司 | Cell-stain apparatus |
| CN103562727A (en) * | 2011-05-30 | 2014-02-05 | 株式会社日立高新技术 | Sample treatment device, sample treatment method, and reaction container for use therein |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103562727A (en) * | 2011-05-30 | 2014-02-05 | 株式会社日立高新技术 | Sample treatment device, sample treatment method, and reaction container for use therein |
| CN103558074A (en) * | 2013-10-10 | 2014-02-05 | 广州安必平自动化检测设备有限公司 | Cell-stain apparatus |
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