CN216584960U - Nucleic acid extraction device - Google Patents

Abstract

The present invention relates to a nucleic acid extraction apparatus comprising: a transport module including a carrier liquid mechanism capable of reciprocating in a first direction relative to the substrate; the extraction module comprises a support frame, a liquid transfer mechanism and a gun head detachably assembled on the liquid transfer mechanism, the support frame is connected with one side of the substrate, which is provided with the conveying module, and extends along a second direction, the liquid transfer mechanism can reciprocate in the second direction relative to the support frame, and the liquid transfer mechanism can generate negative pressure for enabling the gun head to absorb liquid in the liquid carrying mechanism or positive pressure for enabling the gun head to discharge the liquid into the liquid carrying mechanism; the magnetic module comprises an installation frame and a magnetic part, the installation frame is connected with the support frame, and the magnetic part can be close to or far away from the gun head in the first direction relative to the installation frame, so that the magnetic part can be switched between a magnetic supply state and a demagnetizing state. The noise is reduced, meanwhile, no liquid is splashed out, and the risk of cross contamination is reduced.

Description

Nucleic acid extraction device

Technical Field

The utility model relates to the technical field of biological detection, in particular to a nucleic acid extraction device.

Background

With the vigorous promotion of the national gene detection and molecular diagnosis technology and the improvement of the national health medical awareness, the development of the nucleic acid extraction technology is promoted vigorously. At present, the requirement of the whole industry on the nucleic acid extraction technology is higher and higher, and the technology is developed from the initial characteristics of low flux, low efficiency, high noise, poor pollution prevention capability and the like to the characteristics of high flux, high efficiency, low noise, strong pollution prevention capability and the like.

Traditional nucleic acid extraction element adopts last magnetism to inhale the extraction mode, and the magnetic rod sheathes in down high frequency motion and can produce very big noise, and has the risk that liquid splashes out, perhaps adopts magnetism to inhale the extraction mode down, probably has reagent to remain during the liquid-transfering, leads to cross contamination. Meanwhile, in the traditional nucleic acid extraction device, one extraction reagent corresponds to one sampling gun head, so that the gun head is seriously wasted.

SUMMERY OF THE UTILITY MODEL

Therefore, it is necessary to provide a nucleic acid extraction device capable of reducing noise, reducing pollution risk and avoiding waste of a tip, aiming at the problems of high noise, easy pollution and serious waste of the tip of the traditional nucleic acid extraction device.

A nucleic acid extraction device comprising:

a substrate;

the conveying module is arranged on one side of the substrate and comprises a liquid carrying mechanism, and the liquid carrying mechanism can reciprocate in a first direction relative to the substrate;

the extraction module comprises a support frame, a liquid transfer mechanism and a gun head detachably assembled on the liquid transfer mechanism, the support frame is connected with one side of the substrate, which is provided with the conveying module, and the support frame extends along a second direction; the liquid transfer mechanism can reciprocate in the second direction relative to the support frame and can generate negative pressure for enabling the gun head to suck liquid in the liquid carrying mechanism or positive pressure for enabling the gun head to discharge the liquid into the liquid carrying mechanism; the second direction intersects the first direction;

the magnetic suction module comprises a mounting frame and a magnetic suction piece, the mounting frame is connected with the supporting frame, and the magnetic suction piece can move in the first direction relative to the mounting frame to be close to or far away from the gun head so that the magnetic suction piece can be switched between a magnetic supply state for providing magnetic force for magnetic beads in the gun head and a demagnetizing state for removing the magnetic force of the magnetic beads in the gun head.

Above-mentioned nucleic acid extraction element does not need the bar magnet to sheathe upper and lower high frequency motion and carries out the mixing to liquid, consequently, does not have the liquid spill when noise reduction, has reduced cross contamination's risk. Meanwhile, in the nucleic acid extraction device in the prior art, one extraction reagent corresponds to one sampling gun head, so that the serious waste of the gun head is caused. In addition, the nucleic acid extraction device that this embodiment provided, magnetism inhale the module and pass through the mounting bracket assembly in extracting module 30 on, make the integrated level of whole device higher, it is more convenient to use.

In one embodiment, the pipetting mechanism comprises a pipetting part and a plurality of connecting parts, all the connecting parts are connected with the pipetting part and are sequentially arranged in a third direction, a flow channel is arranged in the pipetting part corresponding to one connecting part, and one connecting part is used for matching one gun head; the third direction intersects both the first direction and the second direction;

one gun head corresponds to at least one magnetic part; all the magnetic parts are integrally formed.

In one embodiment, the magnetic module further includes a connecting frame, the connecting frame is slidably connected to the mounting frame, the magnetic member is detachably connected to the connecting frame, and the position of the magnetic member relative to the connecting frame in the second direction is adjustable.

In one embodiment, a waist-shaped hole extending in the second direction is formed in the connecting frame, and the position of the magnetic attraction piece in the second direction is adjustable through the waist-shaped hole.

In one embodiment, the liquid carrying mechanism includes a heating base and a liquid carrying plate, the liquid carrying plate is detachably disposed on the heating base, and the heating base can drive the liquid carrying plate to reciprocate in the first direction relative to the substrate;

the heating seat is provided with a heating part, the liquid carrying plate comprises a cracking part with cracking holes and an eluting part with eluting holes, and the heating part is arranged on the cracking part and the eluting part correspondingly.

In one embodiment, the carrier plate is a 96-well plate.

In one embodiment, the conveying module further comprises a conveying part and a gun head placing frame for placing the gun head, the liquid carrying mechanism and the gun head placing frame are both arranged on the conveying part, and the conveying part can drive the liquid carrying mechanism and the gun head placing frame to reciprocate in the first direction relative to the base plate.

In one embodiment, the conveying module further includes a first lead screw motor, the first lead screw motor is disposed on the substrate, and the conveying member is in threaded connection with a lead screw of the first lead screw motor.

In one embodiment, the extraction module further includes a stepping motor, a synchronous belt, a first synchronous wheel and a second synchronous wheel, the stepping motor and the first synchronous wheel are both disposed on the support frame, the second synchronous wheel is connected to the driving end of the stepping motor, the synchronous belt is sleeved outside the first synchronous wheel and the second synchronous wheel, and the liquid-moving mechanism is connected to the synchronous belt, so that the liquid-moving mechanism and the synchronous belt move synchronously.

In one embodiment, the magnetic module further includes a second screw motor, the second screw motor is connected to the mounting frame, and the magnetic member is connected to a screw of the second screw motor through a screw thread.

Drawings

FIG. 1 is a schematic diagram of a nucleic acid isolation apparatus according to an embodiment of the present invention;

FIG. 2 is a view showing the construction of the nucleic acid extracting apparatus shown in FIG. 1 from another perspective;

FIG. 3 is a schematic diagram showing the construction of a transport module and a substrate of the nucleic acid isolation apparatus shown in FIG. 1;

FIG. 4 is a structural diagram of an extraction module of the nucleic acid extraction apparatus shown in FIG. 1;

FIG. 5 is a diagram showing the structure of a magnetic module of the nucleic acid extraction apparatus shown in FIG. 1;

FIG. 6 is a partial exploded view of the structure shown in FIG. 3;

FIG. 7 is a partial exploded view of the extraction module shown in FIG. 4;

fig. 8 is a block diagram of another perspective of the extraction module shown in fig. 4.

Reference numerals:

100. a nucleic acid extraction device; 10. a substrate; 20. a delivery module; 21. a liquid carrying mechanism; 211. a heating base; 2111. a heating section; 2112. an accommodating cavity; 2113. a containing groove; 212. a liquid carrying plate; 22. a conveying member; 23. a gun head placing frame; 24. a first lead screw motor; 25. a first slide rail; 26. a roller; 27. a drag chain; 30. an extraction module; 31. a support frame; 32. a pipetting mechanism; 321. a liquid transfer section; 322. a connecting portion; 33. a gun head; 34. a stepping motor; 35. a synchronous belt; 36. a first synchronizing wheel; 37. a second synchronizing wheel; 38. a second slide rail; 40. a magnetic module; 41. a mounting frame; 42. a magnetic member; 43. a connecting frame; 431. a kidney-shaped hole; 44. mounting a plate; 45. a second lead screw motor; 46. and a third slide rail.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless explicitly specified otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 and 2, an embodiment of the utility model provides a nucleic acid extraction apparatus 100, which includes a substrate 10, a transportation module 20, an extraction module 30, and a magnetic module 40. The substrate 10 serves as a mounting base, and the transport module 20 is used for transporting a sample and for transporting an extraction liquid for extracting nucleic acid in the sample. The extraction module 30 is capable of generating a negative pressure to draw the liquid within the delivery module 20, and the extraction module 30 is also capable of generating a positive pressure to expel the liquid into the delivery module 20 to cause the liquids to mix thoroughly. Magnetism module 40 of inhaling can make the magnetic bead adsorb on the inner wall that draws module 30, conveniently draws module 30 and discharges the waste liquid, and magnetism module 40 of inhaling can also make the magnetic bead and draw the inner wall separation of module 30, makes and draws module 30 and can normally carry out the liquid mixing work.

The conveying module 20, the extracting module 30 and the magnetic module 40 are all located on the same side of the substrate 10. Referring to fig. 3, the transportation module 20 includes a liquid carrying mechanism 21, and the liquid carrying mechanism 21 is capable of reciprocating in a first direction relative to the substrate 10 to transport the sample and the extraction liquid, so that the extraction module 30 can suck the liquid from the liquid carrying mechanism 21 or discharge the liquid into the liquid carrying mechanism 21.

Referring to fig. 4, the extraction module 30 includes a support frame 31, a pipetting mechanism 32, and a tip 33, wherein the tip 33 is detachably disposed on the pipetting mechanism 32. The support frame 31 extends along a second direction, and the pipetting mechanism 32 can reciprocate in the second direction relative to the support frame 31, so that the gun head 33 assembled on the pipetting mechanism 32 reciprocates in the second direction, so that the gun head 33 extends into the liquid carrying mechanism 21 to suck or discharge liquid into the liquid carrying mechanism 21, and the gun head 33 is separated from the liquid carrying mechanism 21. The pipetting mechanism 32 can generate a negative pressure that causes the tip 33 to aspirate the liquid in the liquid carrying mechanism 21 or a positive pressure that causes the tip 33 to discharge the liquid into the liquid carrying mechanism 21.

In one embodiment, the first direction is perpendicular to the second direction. As shown in fig. 1, the first direction is the X direction in fig. 1, and the second direction is the Z direction in fig. 1. It should be understood that, in other embodiments, the first direction and the second direction may be disposed at other angles, which is not limited herein.

Referring to fig. 5, the magnetic module 40 includes a mounting frame 41 and a magnetic element 42, the mounting frame 41 is connected to the supporting frame 31 of the extraction module 30, and the magnetic element 42 can move in a first direction to be close to or away from the tip 33 relative to the mounting frame 41, so that the magnetic element 42 is switched between a magnetic supply state for providing a magnetic force to the magnetic beads in the tip 33 and a demagnetizing state for removing the magnetic force from the magnetic beads in the tip 33.

When extracting nucleic acid, the nucleic acid extraction apparatus 100 firstly manually adds a mixture of a sample, proteinase K and magnetic beads into the liquid carrying mechanism 21, and pre-packages both the washing solution and the eluent into the liquid carrying mechanism 21. The liquid carrying mechanism 21 moves to a designated position in the first direction, the pipetting mechanism 32 drives the gun head 33 to move in the second direction, the gun head 33 is inserted into the liquid carrying mechanism 21, the pipetting mechanism 32 generates negative pressure, liquid is sucked into the gun head 33, the pipetting mechanism 32 generates positive pressure, and the gun head 33 discharges the liquid into the liquid carrying mechanism 21 so as to mix the liquid for extracting nucleic acid. When waste liquid needs to be discharged in the nucleic acid extraction process, the magnetic attraction piece 42 moves close to the gun head 33 in the first direction, so that the magnetic beads are attracted to the inner wall of the gun head 33, and the liquid transfer mechanism 32 generates positive pressure to discharge the waste liquid into the liquid carrying mechanism 21.

According to the nucleic acid extraction device 100 provided by the embodiment, the magnetic beads are kept in the gun head 33 and are blown and uniformly mixed by the liquid transfer mechanism 32, and the magnetic rod sleeve does not need to move up and down at high frequency, so that the noise is reduced, meanwhile, liquid is not splashed out, and the risk of cross contamination is reduced. Meanwhile, in the nucleic acid extraction device 100 in the prior art, one extraction reagent corresponds to one sampling gun head 33, so that the gun head 33 is seriously wasted, in the nucleic acid extraction device 100 in the application, a mixture of a sample, proteinase K and magnetic beads is manually added, and washing liquid and eluent are pre-packaged, so that the gun head 33 does not need to be replaced in a single extraction process, resources are saved, and the extraction efficiency is improved. In addition, in the nucleic acid extraction device 100 provided by the embodiment, the magnetic module 40 is assembled on the extraction module 30 through the mounting frame 41, so that the integration level of the whole device is higher, and the use is more convenient.

Referring to fig. 6, the conveying module 20 further includes a conveying element 22 and a gun head placing frame 23 for placing the gun head 33, the liquid carrying mechanism 21 and the gun head placing frame 23 are both disposed on the conveying element 22, and the conveying element 22 can drive the liquid carrying mechanism 21 and the gun head placing frame 23 to reciprocate in the first direction relative to the substrate 10. The gun head 33 can be manually placed on the gun head placing frame 23, and the pipette mechanism 32 can automatically take the gun head 33 when moving downwards in the second direction.

The conveying module 20 further includes a first lead screw motor 24, the first lead screw motor 24 is disposed on the substrate 10, and the conveying member 22 is in threaded connection with a lead screw of the first lead screw motor. When the first screw motor 24 works, the screw rod rotates, and the conveying member 22 can be driven to move in the first direction when the screw rod rotates due to the fact that the conveying member 22 is in threaded connection with the screw rod. It should be understood that in other embodiments, transport module 20 may drive transport 22 to reciprocate in the first direction via other mechanisms.

In one embodiment, the conveying module 20 further includes a first slide rail 25 and a first groove, one of the first slide rail 25 and the first groove is disposed on the substrate 10, and the other one is disposed on the conveying element 22, and the first groove and the first slide rail 25 cooperate to facilitate the reciprocating movement of the conveying element 22 relative to the substrate 10 in the first direction. Specifically, the conveying member 22 is provided with a roller 26, the roller 26 is in contact with the substrate 10, and the conveying member 22 is facilitated to slide in the first direction relative to the substrate 10 by the roller 26. In particular, the conveyor module 20 also comprises a drag chain 27 for protecting the cables.

In one embodiment, the liquid carrying mechanism 21 includes a heating base 211 and a liquid carrying plate 212, the heating base 211 is disposed on the conveying element 22, the liquid carrying plate 212 is detachably disposed on the heating base 211, and the conveying element 22 drives the heating base 211 and the liquid carrying plate 212 to reciprocate in a first direction. The liquid carrying plate 212 is separated from the heating seat 211, so that different liquid carrying plates 212 can be replaced for extracting a plurality of samples.

Further, the heating seat 211 is convexly provided with a heating portion 2111, the carrier liquid plate 212 includes a cracking portion provided with cracking holes and an elution portion provided with elution holes, and the cracking portion and all elution portions are correspondingly provided with the heating portion 2111. Thus, the heating unit 2111 heats the cleavage section and the elution section, thereby increasing the cleavage and elution rates.

It should be noted that, when the carrier plate 212 is placed on the heating seat 211, the wells for extracting nucleic acid (e.g., lysis well, washing well, and elution well) are arranged in sequence in the first direction.

The heating seat 211 is provided with a containing cavity 2112 with an open top end, the heating portion 2111 is arranged on the bottom wall of the containing cavity 2112, the heating portion 2111 is recessed with a containing groove 2113, and the bottoms of the cracking portion and the elution portion of the liquid carrying plate 212 are contained in the containing groove 2113, so as to increase the contact area of the heating portion 2111 with the cracking portion and the elution portion, and accelerate cracking and elution.

In one embodiment, the carrier plate 212 is a 96-well plate, and the 96-well plate has 12 rows of wells in a first direction and 8 rows of wells in a third direction (Y direction in fig. 1) intersecting both the first direction and the second direction, i.e., 96 wells. Of course, in other embodiments, the type of the liquid carrying plate 212 is not limited.

Referring to fig. 7, the pipetting mechanism 32 includes a pipetting portion 321 and a plurality of connecting portions 322, all the connecting portions 322 are connected to the pipetting portion 321 and are sequentially arranged in a third direction, a flow channel is provided in the pipetting portion 321 corresponding to one connecting portion 322, and one connecting portion 322 is used for matching one gun head 33. When the liquid-carrying plate 212 is a 96-well plate, the pipetting mechanism 32 has 8 connecting parts 322, and the pipetting part 321 has 8 flow paths, and in this case, the pipetting mechanism 32 can mount 8 tips 33 in the third direction, and in this case, the nucleic acid isolation apparatus 100 can simultaneously perform nucleic acid isolation on 8 samples, thereby improving the efficiency of nucleic acid isolation.

It should be understood that in other embodiments, two 96-deep well plates may be provided in the third direction, and the nucleic acid extraction device 100 can perform nucleic acid extraction on 16 samples at the same time. Of course, in other implementations, the nucleic acid extraction device 100 can also perform nucleic acid extraction on 32 samples simultaneously.

It should be noted that, when 8 tips 33 are installed on the pipetting mechanism 32, in the third direction, the tip placement frame 23 has 8 placement positions for placing the tips 33; when the pipetting mechanism 32 is provided with 16 tips 33, the tip placement frame 23 has 16 placement positions for placing the tips 33 in the third direction. In other words, the number of the placement positions on the tip placement frame 23 in the third direction is equal to the number of the connection portions 322 of the pipetting mechanism 32. It should also be noted here that in the first direction, the lance tip placement frame 23 has a plurality of discharge positions.

Referring to fig. 8, in an embodiment, the extracting module 30 further includes a stepping motor 34, a synchronous belt 35, a first synchronous wheel 36 and a second synchronous wheel 37, the stepping motor 34 and the first synchronous wheel 36 are both disposed on the supporting frame 31, the second synchronous wheel 37 is connected to the driving end of the stepping motor 34, the synchronous belt 35 is sleeved outside the first synchronous wheel 36 and the second synchronous wheel 37, and the liquid-transferring mechanism 32 is connected to the synchronous belt 35, so that the liquid-transferring mechanism 32 and the synchronous belt 35 move synchronously. The stepping motor 34 moves to drive the second synchronizing wheel 37 connected with the stepping motor to move, the synchronous belt 35 sleeved outside the second synchronizing wheel 37 is linked with the first synchronizing wheel 36 and the second synchronizing wheel 37, and the synchronous belt 35 moves to drive the liquid-transferring mechanism 32 to move in the second direction. It is understood that in other embodiments, the pipetting mechanism 32 may be driven in other manners, and is not limited thereto.

Further, the extracting module 30 further includes a second slide rail 38 (see fig. 7) and a second groove, one of the second slide rail 38 and the second groove is disposed on the supporting frame 31, and the other one is disposed on the pipetting mechanism 32, and the second groove and the second slide rail 38 cooperate to facilitate the pipetting mechanism 32 to reciprocate relative to the supporting frame 31 in the second direction.

In one embodiment, the extraction module 30 further comprises a tip removing mechanism connected to the pipetting mechanism 32 and at least partially movable relative to the pipetting mechanism 32 to remove the tips 33 from the pipetting mechanism 32.

In one embodiment, in the first direction, one lance tip 33 corresponds to at least one magnetic element 42, and all magnetic elements 42 are integrally formed to facilitate manufacturing. The magnetic module 40 further includes a connecting frame 43, the connecting frame 43 is slidably connected to the mounting frame 41, the magnetic member 42 is detachably connected to the connecting frame 43, and the magnetic member 42 is adjustable in position in the second direction relative to the connecting frame 43, so as to adjust the position of the magnetic member 42 in the second direction, and ensure that the position of the magnetic member 42 in the second direction matches with the lance head 33.

Referring to fig. 5, a waist-shaped hole 431 extending in the second direction is formed on the connecting frame 43, and the position of the magnetic attraction member 42 in the second direction is adjustable through the waist-shaped hole 431. Of course, in other embodiments, the position of the magnetic attraction member 42 in the second direction can be adjusted by other means, for example, the connecting frame 43 is provided with a plurality of holes at intervals in the second direction, and when the magnetic attraction member 42 is required to be at a certain height, the magnetic attraction member is assembled with the connecting frame 43 through the corresponding holes.

The magnetic module 40 further comprises a mounting plate 44, the magnetic member 42 is disposed on the mounting plate 44, and the mounting plate 44 is connected to the connecting frame 43. The magnetic module 40 further comprises a second screw motor 45, the second screw motor 45 is connected with the mounting frame 41, and the magnetic member 42 is in threaded connection with a screw of the second screw motor 45 through a connecting frame 43. When the second screw motor 45 works, the screw rod rotates, and the connecting frame 43 can be driven to move in the first direction when the screw rod rotates due to the threaded connection of the connecting piece and the screw rod, so that the mounting plate 44 and the magnetic attraction piece 42 are driven to move in the first direction.

The magnetic module 40 further includes a third slide rail 46 and a third groove, one of the third slide rail 46 and the third groove is disposed on the mounting frame 41, the other is disposed on the connecting frame 43, and the third groove and the third slide rail 46 are matched to facilitate the connecting frame 43 to slide in a reciprocating manner in the first direction relative to the mounting frame 41.

The operation principle of the nucleic acid extraction device 100 provided by the embodiment of the utility model is as follows:

under the drive of the first lead screw motor, the heating seat 211 and the gun head placing frame 23 move to a first designated position in the first direction, and an operator places the processed 96 deep hole plate and the gun head 33 in place. The first screw motor 24 drives the gun head placing frame 23 to move to the position of taking the gun head 33, the stepping motor 34 drives the liquid moving mechanism 32 and the gun head 33 to move downwards in the second direction to take the gun head 33, and after the gun head 33 is taken, the stepping motor 34 drives the liquid moving mechanism 32 to move upwards to the second designated position. The first screw motor drives the heating seat 211 with the 96-deep-hole plate to move to a third designated position, the stepping motor 34 drives the liquid transferring mechanism 32 and the gun head 33 to move downwards in the second direction, so that the gun head 33 is inserted into the cracking hole of the 96-deep-hole plate, negative pressure is generated in the liquid transferring mechanism 32, the gun head 33 sucks mixed liquid, and the gun head 33 returns. The first screw motor 24 drives the 96 deep hole plate to move to a washing hole positioned below the gun head 33, the stepping motor 34 drives the liquid transferring mechanism 32 and the gun head 33 to move downwards in the second direction, so that the gun head 33 is inserted into the washing hole of the 96 deep hole plate, positive pressure is generated in the liquid transferring mechanism 32, a mixed liquid of a sample and magnetic beads in the gun head 33 is discharged into the washing hole, and then the mixed liquid and the washing liquid are uniformly mixed by switching the positive pressure and the negative pressure of the liquid transferring mechanism 32.

After uniform mixing, all the washed liquid is sucked into the gun head 33, the gun head 33 returns, and the second screw motor 45 drives the magnetic attraction piece 42 to be close to the gun head 33, so that the magnetic beads are adsorbed in the inner wall of the gun head 33. The first screw motor 24 drives the 96 deep hole plate to move to a waste liquid hole positioned below the gun head 33, and the gun head 33 spits the waste liquid into the waste liquid hole.

The first screw motor 24 drives the 96 deep hole plate to move until the elution hole is positioned below the gun head 33, and then the same steps as the washing hole are repeated to fully mix the mixed liquid with the eluent. The liquid formed after elution is then transferred to the next well. The operator removes the 96-well plate from the heating block 211 and the single extraction is completed.

Here, the single extraction can realize extraction of nucleic acids in 1 sample or extraction of nucleic acids in a plurality of samples.

The nucleic acid extraction device 100 provided by the embodiment of the utility model has the following beneficial effects:

1. the liquid-transfering mechanism 32 sucks the mixed liquid of magnetic beads into the gun head 33, the second screw motor 45 drives the magnetic-attracting piece 42 to be close to the outer wall of the gun head 33, the magnetic beads are gathered on the inner wall of the gun head 33 under the adsorption effect of magnetic force, then the gun head 33 spits out waste liquid under the effect of the liquid-transfering mechanism 32, the magnetic beads are retained in the gun head 33, the magnetic-attracting mode (middle magnetic attraction) does not involve the upper and lower high frequency of the magnetic rod sleeve, no liquid is splashed out when the noise is reduced, and the risk of cross contamination is reduced.

2. The sample, the protease K and the magnetic bead mixture are manually added, and the washing liquid and the eluent are pre-packaged, so that the gun head 33 does not need to be replaced in a single extraction process, resources are saved, and the extraction efficiency is improved.

3. The conveying module 20, the extracting module 30 and the magnetic attracting module 40 are all disposed on the substrate 10, and mainly relate to the movement in the first direction and the second direction, so that the whole nucleic acid extracting apparatus 100 has high integration degree and simple structure.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A nucleic acid extraction apparatus, comprising:

a substrate (10);

the conveying module (20) is arranged on one side of the substrate (10) and comprises a liquid carrying mechanism (21), and the liquid carrying mechanism (21) can reciprocate in a first direction relative to the substrate (10);

the extraction module (30) comprises a support frame (31), a liquid transfer mechanism (32) and a gun head (33) detachably assembled on the liquid transfer mechanism (32), the support frame (31) is connected with one side, provided with the conveying module (20), of the substrate (10), and the support frame (31) extends along a second direction; the liquid transfer mechanism (32) can reciprocate in the second direction relative to the support frame (31) and can generate negative pressure for enabling the gun head (33) to suck the liquid in the liquid carrying mechanism (21) or positive pressure for enabling the gun head (33) to discharge the liquid into the liquid carrying mechanism (21); the second direction intersects the first direction;

module (40) is inhaled to magnetism, including mounting bracket (41) and magnetism piece (42), mounting bracket (41) with support frame (31) are connected, it can be for mounting bracket (41) for the piece (42) is inhaled to magnetism moves in first direction in order to be close to or keep away from rifle head (33), so that it provides the confession magnetism state of magnetic force and gets rid of the switching between the demagnetization state to being located the magnetic force of the magnetic bead in rifle head (33) to be located to magnetism piece (42).

2. The nucleic acid extraction apparatus according to claim 1, wherein the pipetting mechanism (32) includes a pipetting portion (321) and a plurality of connecting portions (322), all of the connecting portions (322) are connected to the pipetting portion (321) and arranged in sequence in a third direction, a flow channel is provided in the pipetting portion (321) corresponding to one of the connecting portions (322), and one of the connecting portions (322) is adapted to fit one of the tips (33); the third direction intersects both the first direction and the second direction;

wherein one lance head (33) corresponds to at least one magnetic part (42); all the magnetic suction pieces (42) are integrally formed.

3. The nucleic acid extraction device according to claim 1, wherein the magnetic module (40) further comprises a connecting frame (43), the connecting frame (43) is slidably connected with the mounting frame (41), the magnetic member (42) is detachably connected with the connecting frame (43), and the position of the magnetic member (42) relative to the connecting frame (43) in the second direction is adjustable.

4. The nucleic acid extraction apparatus according to claim 3, wherein a waist-shaped hole (431) extending in the second direction is provided in the connecting rack (43), and the position of the magnetic attraction member (42) in the second direction is adjustable through the waist-shaped hole (431).

5. The nucleic acid extraction apparatus according to claim 1, wherein the liquid carrying mechanism (21) comprises a heating base (211) and a liquid carrying plate (212), the liquid carrying plate (212) is detachably disposed on the heating base (211), and the heating base (211) can drive the liquid carrying plate (212) to reciprocate in the first direction relative to the substrate (10);

the heating seat (211) is provided with a heating part (2111), the liquid carrying plate (212) comprises a cracking part with cracking holes and an elution part with elution holes, and the cracking part and the elution part are correspondingly provided with the heating part.

6. The nucleic acid extraction apparatus according to claim 5, wherein the carrier liquid plate (212) is a 96-well plate.

7. The nucleic acid extraction device according to claim 1, wherein the conveying module (20) further comprises a conveying member (22) and a gun head placing frame (23) for placing a gun head (33), the liquid carrying mechanism (21) and the gun head placing frame (23) are both arranged on the conveying member (22), and the conveying member (22) can drive the liquid carrying mechanism (21) and the gun head placing frame (23) to reciprocate in the first direction relative to the substrate (10).

8. The nucleic acid extraction apparatus according to claim 7, wherein the transport module (20) further includes a first screw motor (24), the first screw motor (24) is provided on the substrate (10), and the transport member (22) is screw-coupled to a screw of the first screw motor (24).

9. The nucleic acid extraction device according to claim 1, wherein the extraction module (30) further comprises a stepping motor (34), a synchronous belt (35), a first synchronous wheel (36) and a second synchronous wheel (37), the stepping motor (34) and the first synchronous wheel (36) are both disposed on the support frame (31), the second synchronous wheel (37) is connected to the driving end of the stepping motor (34), the synchronous belt (35) is sleeved outside the first synchronous wheel (36) and the second synchronous wheel (37), and the pipetting mechanism (32) is connected to the synchronous belt (35) so that the synchronous belt (35) and the pipetting mechanism (32) move synchronously.

10. The nucleic acid extraction device of claim 1, wherein the magnetic module (40) further comprises a second screw motor, the second screw motor is connected with the mounting frame (41), and the magnetic member (42) is in threaded connection with a screw of the second screw motor.

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