WO2019174471A1

WO2019174471A1 - Pipetting mechanism

Info

Publication number: WO2019174471A1
Application number: PCT/CN2019/076461
Authority: WO
Inventors: 易初丽
Original assignee: 武汉医蒂生物科技有限公司
Priority date: 2018-03-13
Filing date: 2019-02-28
Publication date: 2019-09-19
Also published as: CN208574655U

Abstract

A pipetting mechanism comprises an infusion device, a mixing device, a liquid receiving plate (1), a spacer device, and a mounting rack (2). The infusion device comprises: an infusion set (3), an infusion set mounting rack (3a), an infusion set driving unit (3b) and a plurality of infusion units, wherein the infusion set (3) comprises sealed chambers (5) that are in one-to-one correspondence with the plurality of infusion units; the sealed chambers (5) are mounted side by side to each other; a plurality of needles (6) arranged corresponding to the liquid receiving plate (1) are mounted at a bottom part of each of the sealed chambers (5); and each of the needles (6) respectively communicates with a respective corresponding sealed chamber (5). The infusion set (3) is mounted at a bottom part of the infusion set mounting rack (3a); the infusion set driving unit (3b) is fixed onto the mounting rack (2), and the infusion set driving unit (3b) is configured to drive the infusion set mounting rack (3a) to move the infusion set (3) up and down. Each infusion unit comprises a reagent supply device (8) and a reagent flow controller (9). The mixing device comprises a mixing plate (14), a mixing plate mounting rack (14a), and a mixing plate driving device (14b). The spacer device comprises a spacer (17) and a spacer driving device (17a); and the spacer driving device (17a) is configured to drive the spacer (17) to move horizontally below a mixing rod (4), thus avoiding the problem of pollution during the pipetting process.

Description

Pipetting mechanism

The present application claims priority to Chinese Patent Application No. 20, 182, 034, 649, filed on March 13, 2018, the entire disclosure of which is hereby incorporated by reference.

Technical field

The present application relates to the field of experimental instruments, and in particular to a pipetting mechanism.

Background technique

Pipetting operations often occur in biomedical and chemical experiments. In order to facilitate the high-throughput transfer of chemical reagents, automatic pipetting stations are often used instead of manual pipettes and a large number of high quality tips are used for automatic transfer of chemical reagents.

In the process of implementing the present application, the designer found that the prior art has at least the following problems:

The automatic pipetting station has a very slow pipetting speed when transferring chemical reagents, and the cost of pipetting is high and it is easy to cause cross-contamination. For example, when transferring 6 different reagents into a 96-well plate, the automatic pipetting station machinery The arm needs to be operated as follows: accurately insert and lift 96 tips, inhale the reagent, release the reagent, mix the reagent, discard the tip, and repeat the above operation 5 times for transferring 6 different chemical reagents. The process is slow. And the tip needs to have a higher quality, otherwise the robotic arm cannot accurately insert and extract and inhale the reagent. It can be seen that the pipetting process of the automatic pipetting station is cumbersome and the pipetting process is slow, which reduces the working efficiency of the pipetting; at the same time, a total of 576 high-quality tips are consumed in the process, and the quality is high. The higher cost of the tip increases the cost of pipetting; in addition, the biggest risk or hidden danger of this pipetting method is the cross-contamination generated in the pipetting path: when the robot arm lifts 96 tips, inhaling reagents from the reagent disk, Move to a 96-well plate to release and mix the reagents, move to the trash can and discard the tip. There is no baffle in the pipetting path to block the liquid that may drip from the tip or the loose tip. Cross contamination caused by the 96 board.

Summary of the invention

In order to solve the problem that the liquid-filling work of the automatic pipetting workstation in the prior art is low in efficiency, high in cost, and easy to cause pollution, the embodiment of the present application provides a pipetting mechanism. The technical solution is as follows:

The embodiment of the present application provides a pipetting mechanism, the pipetting mechanism includes: an infusion device, a mixing device, a liquid receiving plate, a baffle device, and a mounting frame, and the infusion device includes: an infusion device, an infusion set The infusion set driving unit and the plurality of infusion units, the infusion set includes a sealed cavity corresponding to the plurality of infusion units, the sealed chambers being installed side by side, and the bottom of each of the sealed chambers is installed Having a plurality of needles disposed corresponding to the cells of the liquid receiving plate, each of the needles being in communication with a respective one of the sealed chambers, the infusion set being mounted at a bottom of the infusion set, An infusion set drive unit is secured to the mount, the infusion set drive unit configured to drive the infusor mount to move the infusion set up and down.

The plurality of infusion units each include: a reagent supply and a reagent flow controller, and for any one of the infusion units, a liquid outlet of the reagent supply is in communication with a liquid inlet of the reagent flow controller, A liquid outlet of the reagent flow controller is in communication with each of the sealed chambers.

The mixing device comprises: a mixing plate, a mixing plate mounting frame and a mixing plate driving device, wherein the mixing plate mounting frame is fixed on the mounting frame, and the mixing plate driving device is driven and installed in the On the mixing plate mounting frame, the mixing plate is installed at the bottom of the mixing plate mounting frame, and the bottom of the mixing plate is provided with a mixing rod corresponding to the hole of the liquid receiving plate. The mixing plate driving device is configured to drive the mixing plate mounting frame to move the mixing rod up and down within the cells of the liquid receiving plate;

The partition device includes: a partition plate and a partition driving device, the partition plate is disposed under the mixing rod, the partition driving device is fixed on the mounting bracket, and the partition driving device It is configured to drive the diaphragm to move horizontally below the mixing rod.

Specifically, the liquid receiving plate is a 1-well plate, a 4-well plate, a 6-well plate, a 12-well plate, a 24-well plate, a 96-well plate or a 384-well plate.

Further, the pipetting mechanism further includes: a slide rail and a limit card frame, the mixing plate is arranged side by side with the infusion device, and the sliding rail is arranged along the direction of the mixing plate and the infusion set Provided on the mounting frame, the limit card frame is slidably mounted on the slide rail, a card slot is disposed on a top surface of the limit card frame, and the liquid plate is mounted on the card slot Inside.

Further, the pipetting mechanism further includes: a limit card frame driving device, the limit card frame driving device includes: a belt, a pulley, a fixing rod and a motor fixed on the mounting frame, the fixing rod is fixed On the side wall of the slide rail, the pulley is two, and the two pulleys are respectively mounted on an output shaft of the motor and the fixing rod, and the belt is set on two of the pulleys. And the belt is arranged in parallel with the slide rail, and the limit card frame is fixed on the belt.

Further, the infusion set driving unit comprises: an infusion set driving motor, an infusion set driving mounting plate, an infusion set belt, a screw rod, a screw nut block, a screw nut slider and a screw nut sliding rail, and the infusion set a drive mounting plate fixed to the mounting bracket, the infusion set driving motor being fixed on a bottom of the infusion set driving mounting plate, and an output shaft of the infusion set driving motor and the lead screw passing through the The infusion set drives a mounting plate, the infusion set belt is disposed on a top surface of the infusion set drive mounting plate, and the infusion set belt is separately sleeved on an output shaft of the infusion set driving motor and the lead screw Upper, the lead screw nut block is threadedly engaged with the lead screw and the lead screw nut block is fixed on the infusion set mounting bracket, and the lead screw nut slide rail is mounted on the infusion set drive mounting plate The screw nut slider is fixed on the screw nut block, and the screw nut slider is slidably disposed on the screw nut slide rail.

Further, the partition driving device includes: a diaphragm driving motor, a diaphragm driving mounting plate, a partition rotating shaft, a partition belt and a partition mounting rod, and the partition driving mounting plate is fixed on the mounting bracket The baffle drive motor is fixed to a bottom of the baffle drive mounting plate, and an output shaft of the baffle drive motor and the baffle rotation shaft pass through the baffle drive mounting plate, a baffle belt is disposed on a top surface of the baffle drive mounting plate, and the baffle belt is respectively sleeved on an output shaft of the baffle drive motor and the baffle rotating shaft, the baffle One end of the mounting rod is mounted on the baffle belt, and the other end of the baffle mounting rod is mounted at the bottom of the baffle.

Specifically, the plurality of needles are arranged in one-to-one correspondence with each row of cells of the liquid receiving plate.

Further, the reagent flow controller is: a peristaltic pump, a diaphragm pump, a metering pump, a syringe pump, a plug pump, a piston pump or a self-priming pump.

Specifically, the infusion set is mounted on a side wall of the mounting bracket, and the reagent supply is disposed in the mounting bracket above the infusion set.

The technical solution provided by the embodiment of the present application has the beneficial effects that the pipetting mechanism can control the infusion condition of each infusion unit through the reagent flow controller, and when the chemical reagent output in a reagent supply is required, the reagent is passed. The flow controller transports the chemical reagent from the reagent supply to the sealed chamber of the infusion set, and realizes high-pressure output through the needle, so that multiple sealed chambers of the infusion set can be connected with different chemical reagents, and the output chemical reagent is selected as needed. The type and volume of the pipetting mechanism provided by the embodiment does not require the use of a pipetting tip or a pipetting station, thereby improving the pipetting speed and efficiency; at the same time, the pipetting mechanism is convenient to clean, so that the pipetting mechanism The ability to replace different chemical reagents and achieve repeated use without the need to replace any consumables further reduces the cost of pipetting; in addition, the use of baffles makes the reagents safe and reliable in the pipetting path, thereby avoiding the risk of cross-contamination.

DRAWINGS

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present application. Other drawings may also be obtained from those of ordinary skill in the art in light of the inventive work.

1 is a schematic structural view of a pipetting mechanism provided by an embodiment of the present application;

2 is a schematic structural view of an infusion set according to an embodiment of the present application;

3 is a schematic structural view of an infusion set, an infusion set mounting frame, and an infusion set driving unit according to an embodiment of the present application;

4 is a schematic structural view of a partition plate and a partition driving device provided by an embodiment of the present application;

FIG. 5 is a schematic structural diagram of a slide rail and a limit card frame provided by an embodiment of the present application.

detailed description

In order to make the objects, technical solutions and advantages of the present application more clear, the embodiments of the present application will be further described in detail below with reference to the accompanying drawings.

Example

The embodiment of the present application provides a pipetting mechanism. As shown in FIG. 1 , the pipetting mechanism includes: an infusion device, a mixing device, a liquid receiving plate, a separator device, and a mounting frame 2. The infusion device includes: an infusion device 3. The infusion set 3a, the infusion set drive unit 3b and the plurality of infusion units. In combination with FIG. 2, the infusion set 3 comprises a sealed chamber 5 corresponding to the plurality of infusion units, and the sealed chambers 5 are installed side by side, each The bottoms of the sealed chambers 5 are respectively provided with a plurality of needles 6 arranged corresponding to the liquid receiving plate 1, and each of the needles 6 is respectively connected with a corresponding sealing chamber 5, as shown in Fig. 3, the infusion set 3 is installed in the infusion set. At the bottom of the frame 3a, the infusion set driving unit 3b is fixed to the mounting frame 2, and the infusion set driving unit 3b is configured to drive the infusion set mounting frame 3a to move the infusion set 3 up and down.

Referring again to FIG. 1, each infusion unit includes: a reagent supply 8 and a reagent flow controller 9. For any one of the infusion units, the liquid outlet of the reagent supply 8 is connected to the inlet of the reagent flow controller 9, reagent The liquid outlet of the flow controller 9 is in communication with each of the sealed chambers 5. In implementation, the liquid outlet of the reagent supply 8 and the liquid inlet of the reagent flow controller 9 are communicated through the reagent output tube, and the liquid outlet of the reagent flow controller 9 is also connected to each of the sealed chambers 5 through the reagent output tube. The reagent output tube can be a rubber hose. The rubber hose has a certain flexibility, can adapt to the reagent supply 8, the reagent flow controller 9 and the sealing chamber 5 in different positions, so that the reagent supply 8, the reagent flow controller 9 and the sealing chamber 5 can be arranged reasonably, and the movement can be reduced. The volume of the liquid mechanism.

The mixing device comprises: a mixing plate 14, a mixing plate mounting frame 14a and a mixing plate driving device 14b, the mixing plate mounting frame 14a is fixed on the mounting frame 2, and the mixing plate driving device 14b is driven to be mounted on the mixing plate. On the frame 14a, the mixing plate 14 is installed at the bottom of the mixing plate mounting frame 14a, and the bottom of the mixing plate 14 is provided with a mixing rod 4 corresponding to the cells of the liquid receiving plate 1, and the mixing plate driving device 14b It is configured to drive the mixing plate mount 14a to move the kneading rod 4 up and down within the cells of the liquid receiving plate 1.

Specifically, as shown in FIG. 4, the spacer device includes: a partition plate 17 and a partition driving device 17a. The partition plate 17 is disposed below the mixing rod 4, and the partition driving device 17a is fixed to the mounting frame 2, and The partition driving device 17a is configured to drive the partition plate 17 to move horizontally below the mixing rod 4. After the solution in the liquid-repellent plate 1 is mixed by the mixing plate 14, a small amount of the solution may remain on the mixing rod 4, and the separator 17 is extended by the separator driving device 17a, so that the mixing rod 4 may be The dripped solution is dropped on the partition plate 17, thereby preventing the liquid-repellent plate 1 from moving during the moving process, and the residual solution on the mixing rod 4 is dropped into the other holes of the liquid-collecting plate 1 to avoid the contamination problem during the pipetting process. When the mixing operation needs to be performed again, the partition plate 17 is retracted by the partition driving device 17a. In the implementation, after the mixing rod 4 mixes the solution in the liquid-repellent plate 1, a small amount of the solution may remain on the mixing rod 4, and the separator 17 is extended to make the solution remaining on the mixing rod 4 drop. It falls on the partition plate 17, thereby preventing the liquid-repellent plate 1 from moving during the moving process, and the residual solution on the mixing rod 4 is dropped into the other holes of the liquid-collecting plate 1, thereby avoiding the problem of contamination during the pipetting process.

Specifically, the wetted plate 1 may be a 1-well plate, a 4-well plate, a 6-well plate, a 12-well plate, a 24-well plate, a 96-well plate or a 384-well plate, and is selected according to specific experimental conditions. In implementation, the plurality of needles 5 on each of the sealed chambers 5 are respectively arranged in one-to-one correspondence with the cells of each row of the liquid receiving plate 1, and the plurality of needles 6 on the same sealed chamber 5 are sequentially turned by moving the liquid receiving plate 1. Chemical reagents are added to each column of cells of the wetted plate 1. When it is necessary to add the second chemical reagent, the liquid plate 1 is moved again to cause the plurality of needles 6 on the other sealed chamber 5 to sequentially add the second chemical agent to each column of the liquid plate 1.

Specifically, the infusion set 3 is mounted on the side wall of the mounting frame 2, and the reagent supply 8 is disposed in the mounting frame 2 above the infusion set 3. The mounting bracket 2 is capable of fixing and supporting the infusion set 3. At the same time, the reagent supply 8 is provided with a mounting position.

The infusion set driving unit 3b and the mixing plate driving device 14b provided in the embodiments of the present application may be the same driving structure. As shown in FIG. 3, the infusion set driving unit 3b is briefly introduced as an example, specifically, the infusion set is driven. The unit 3b includes: an infusion set driving motor 3c, an infusion set driving mounting plate 3d, an infusion set belt 3e, a screw rod 3f, a screw nut block 3g, a screw nut slider 3h, and a screw nut slide rail 3i, and the infusion set is driven and installed. The plate 3d is fixed on the mounting frame 2, the infusion set driving motor 3c is fixed on the bottom of the infusion set driving mounting plate 3d, and the output shaft of the infusion set driving motor 3c and the lead screw 3f are both passed through the infusion set driving mounting plate 3d, and the infusion is performed. The belt 3e is disposed on the top surface of the infusion set drive mounting plate 3d, and the infusion set belts 3e are respectively sleeved on the output shaft of the infusion set driving motor 3c and the lead screw 3f, and the screw nut block 3g and the lead screw 3f pass Threaded and screw nut block 3g is fixed on the infusion set 3a, the screw nut slide 3i is mounted on the infusion set drive mounting plate 3d, and the screw nut slider 3h is fixed on the screw nut block 3g, and the wire The rod nut slider 3h is slidably set at 3i on the slide rod nut.

Specifically, the partition driving device 17a includes a partition driving motor 17b, a partition driving mounting plate 17c, a partition rotating shaft 17d, a partition belt 17e, and a partition mounting rod 17f, and the partition driving mounting plate 17c is fixed to the mounting bracket 2, the bulkhead drive motor 17b is fixed to the bottom of the bulkhead drive mounting plate 17c, and the output shaft of the bulkhead drive motor 17b and the diaphragm rotation shaft 17d are both passed through the bulkhead drive mounting plate 17c, and the bulkhead belt 17e is disposed. On the top surface of the partition driving mounting plate 17c, and the partition belt 17e is respectively sleeved on the output shaft of the partition driving motor 17b and the partition rotating shaft 17d, one end of the partition mounting rod 17f is mounted on the partition belt At the 17e, the other end of the partition mounting rod 17f is attached to the bottom of the partition 17.

Further, as shown in FIG. 5, the pipetting mechanism may further include: a slide rail 10 and a limit card frame 23, the mixing plate 14 is arranged side by side with the infusion set 3, and the slide rail 10 is arranged along the mixing plate 14 and the infusion set 3. The direction of the card frame 23 is slidably mounted on the slide rail 10. The top surface of the limit card frame 23 is provided with a card slot 11a, and the liquid plate 1 is snapped onto the card slot 11a. The slide rail 10 allows the limit card frame 23 to smoothly move between the mixing plate 14 and the infusion set 3, thereby facilitating the movement of the liquid receiving plate 1.

Further, the pipetting mechanism further comprises: a limit card frame driving device, the limit card frame driving device comprises a belt 23a, a pulley 23b, a fixing rod 23c and a motor 23d fixed on the mounting frame 13, and the fixing rod 23c is fixed on the sliding rail On the side wall of the 21, there are two pulleys 23b, and the two pulleys 23b are respectively mounted on the output shaft of the motor 23d and the fixed rod 23c, the belt 23a is fitted on the two pulleys 23b, and the belt 23a is arranged in parallel with the slide rail 21, The limit card frame 23 is fixed to the belt 23a. The limit card frame driving device drives the limit card frame 23 to move the automatic operation of the limit card frame 23.

Further, the reagent flow controller 9 may be: a peristaltic pump, a diaphragm pump, a metering pump, a syringe pump, a plug pump, a piston pump or a self-priming pump.

The following briefly describes the working principle of the pipetting mechanism provided in this embodiment, which is as follows:

A plurality of chemical reagents are manually loaded into different chemical reagent suppliers 8, and a liquid receiving plate 1 (single use) is placed in the card slot 11a of the limit card frame 23, and a set of mixing rods is arranged. 4 (single use) is inserted below the mixing plate 14, and the subsequent step of adding and mixing is automated by the apparatus.

The limiting card frame 23 drives the liquid receiving plate 1 to slide along the sliding rail 10 to the lower side of the first row of needles 6 of the infusion set 3 and move equidistantly with the sliding rail 10, so that the liquid receiving plate 1 is in the direction of the sliding rail 10 The first chemical reagent is sequentially received in the hole, and then slides to the lower side of the mixing rod 4, so that the mixing rod 4 protrudes into the cell of each liquid receiving plate 1 to move up and down to reach the mixing liquid plate 1 The reagent in each cell (the liquid in the liquid plate 1 is filled with liquid before receiving the first chemical agent), after the mixing is completed, the mixing rod 4 rises and moves away from the liquid receiving plate 1, and the partition 17 moves. Entering between the mixing rod 4 and the liquid receiving plate 1 to block the liquid that may drip from the mixing rod 4 into the liquid receiving plate 1 to prevent cross-contamination;

The above movement is repeated until all of the desired liquid or chemical reagent is moved into the wetted plate 1 and mixed with each other.

During the pipetting process, when different chemical reagents need to be transferred in the same sealed cavity 5, a certain path through which the chemical reagent flows may be cleaned, and the specific method may include: loading a large number of times in the reagent supply device 8 The pure water, through the high pressure output of the needle 6, achieves the cleaning of the passage during the high-pressure output of a large amount of pure water.

The embodiment of the present application provides a pipetting mechanism, which can control the infusion condition of each infusion unit through a reagent flow controller, and pass the reagent flow controller when a chemical reagent output in a reagent supply is required. The chemical reagent is transported from the reagent supply to the sealed cavity of the infusion set, and the high pressure output is realized through the needle, so that the plurality of sealed chambers of the infusion set are fed with different chemical reagents, and the output chemical reagent is selected according to the need, which makes The pipetting mechanism provided in the embodiment does not need to use a pipetting tip or a pipetting station, thereby reducing the liquid pipetting cost, and the pipetting process is simple and fast, and the pipetting efficiency is improved; at the same time, the pipetting mechanism is convenient to clean. This allows the pipetting mechanism to replace different chemical reagents and achieve repeated use without replacing any consumables, which further reduces the cost of pipetting; in addition, the use of a separator makes the reagents safe and reliable in the pipetting path, thereby avoiding The risk of cross-contamination.

The above is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present application are included in the protection of the present application. Within the scope.

Claims

A pipetting mechanism, characterized in that the pipetting mechanism comprises: an infusion device, a mixing device, a liquid receiving plate, a baffle device and a mounting frame, the infusion device comprising: an infusion set, an infusion set, and an infusion And a plurality of infusion units, the infusion set includes a sealed cavity corresponding to the plurality of infusion units, the sealed chambers being installed side by side, and the bottom of each of the sealed chambers is mounted with a plurality of needles arranged correspondingly to the cells of the liquid receiving plate, each of the needles being respectively in communication with a corresponding sealing cavity, wherein the infusion set is installed at a bottom of the infusion set, the infusion set a drive unit fixed to the mounting bracket, the infusion set driving unit configured to drive the infusion set to move the infusion device up and down;

The plurality of infusion units each include: a reagent supply and a reagent flow controller, and for any one of the infusion units, a liquid outlet of the reagent supply is in communication with a liquid inlet of the reagent flow controller, a liquid outlet of the reagent flow controller is in communication with each of the sealed chambers;

The mixing device comprises: a mixing plate, a mixing plate mounting frame and a mixing plate driving device, wherein the mixing plate mounting frame is fixed on the mounting frame, and the mixing plate driving device is driven and installed in the On the mixing plate mounting frame, the mixing plate is installed at the bottom of the mixing plate mounting frame, and the bottom of the mixing plate is provided with a mixing rod corresponding to the hole of the liquid receiving plate. The mixing plate driving device is configured to drive the mixing plate mounting frame to move the mixing rod up and down within the cells of the liquid receiving plate;

The partition device includes: a partition plate and a partition driving device, the partition plate is disposed under the mixing rod, the partition driving device is fixed on the mounting bracket, and the partition driving device It is configured to drive the diaphragm to move horizontally below the mixing rod.
The pipetting mechanism according to claim 1, wherein the liquid receiving plate is a 1-well plate, a 4-well plate, a 6-well plate, a 12-well plate, a 24-well plate, a 96-well plate or a 384-well plate.
The pipetting mechanism according to claim 2, wherein the pipetting mechanism further comprises: a slide rail and a limit card frame, the mixing plate is arranged side by side with the infusion device, and the slide rail is arranged along the rail The direction of the arrangement of the mixing plate and the infusion set is set on the mounting frame, the limiting card frame is slidably mounted on the sliding rail, and a card slot is arranged on the top surface of the limiting card frame. The liquid receiving plate is snapped into the card slot.
The pipetting mechanism according to claim 3, wherein the pipetting mechanism further comprises: a limit card frame driving device, wherein the limit card frame driving device comprises: a belt, a pulley, a fixing rod and a fixing a motor on the mounting bracket, the fixing rod is fixed on a side wall of the sliding rail, and the pulley is two, and the two pulleys are respectively mounted on an output shaft of the motor and the fixing rod, The belt is set on two of the pulleys, and the belt is arranged in parallel with the slide rail, and the limit card frame is fixed on the belt.
The pipetting mechanism according to claim 1, wherein the infusion set driving unit comprises: an infusion set driving motor, an infusion set driving mounting plate, an infusion set belt, a screw rod, a screw nut block, and a screw nut sliding. a block and a screw nut slide rail, the infusion set driving mounting plate is fixed on the mounting frame, the infusion set driving motor is fixed on a bottom of the infusion set driving mounting plate, and the infusion set drives the motor An output shaft and the lead screw pass through the infusion set drive mounting plate, the infusion set belt is disposed on a top surface of the infusion set drive mounting plate, and the infusion set belt is separately sleeved in the An infusion set drives an output shaft of the motor and the lead screw, the lead screw nut block is threadedly engaged with the lead screw, and the lead screw nut block is fixed on the infusion set mounting frame, the lead screw nut The slide rail is mounted on the infusion set drive mounting plate, the lead screw nut slider is fixed on the lead screw nut block, and the lead screw nut slide is slidably disposed on the lead screw nut slide rail.
The pipetting mechanism according to claim 1, wherein the partition driving device comprises: a diaphragm driving motor, a diaphragm driving mounting plate, a diaphragm rotating shaft, a diaphragm belt, and a partition mounting rod, a baffle drive mounting plate fixed to the mounting bracket, the baffle drive motor being fixed on a bottom of the baffle drive mounting plate, and an output shaft of the baffle drive motor and the baffle rotating shaft Driving the mounting plate through the partition plate, the baffle belt is disposed on a top surface of the baffle drive mounting plate, and the baffle belt is separately sleeved on an output shaft of the baffle drive motor and On the rotating shaft of the partition plate, one end of the partition mounting rod is mounted on the partition belt, and the other end of the partition mounting rod is mounted on the bottom of the partition.
The pipetting mechanism according to claim 1, wherein said plurality of needles on each of said sealed chambers are respectively arranged in one-to-one correspondence with each of the rows of cells of said liquid receiving plate.
The pipetting mechanism according to any one of claims 1 to 7, wherein the reagent flow controller is: a peristaltic pump, a diaphragm pump, a metering pump, a syringe pump, a plug pump, a piston pump or a self-priming pump .
The pipetting mechanism according to claim 1, wherein said infusion set is mounted on a side wall of said mounting bracket, and said reagent supply is disposed in said mounting bracket above said infusion set.