CN108097341A - A kind of automatic ration gradient sample adding device - Google Patents
A kind of automatic ration gradient sample adding device Download PDFInfo
- Publication number
- CN108097341A CN108097341A CN201810014825.7A CN201810014825A CN108097341A CN 108097341 A CN108097341 A CN 108097341A CN 201810014825 A CN201810014825 A CN 201810014825A CN 108097341 A CN108097341 A CN 108097341A
- Authority
- CN
- China
- Prior art keywords
- suction pipe
- suction
- sample
- casing
- piston
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/0237—Details of electronic control, e.g. relating to user interface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
Abstract
A kind of automatic ration gradient sample adding device, including control assembly, piston component and multiple suction nozzles, suction nozzle includes suction pipe and casing, casing is sleeved on the sleeve of piston component lower end, suction pipe passes perpendicularly through the bottom surface of casing and is fixed on bottom surface, and the upper port of suction pipe is higher than the bottom surface of casing and does not connect with the bottom surface of sleeve.Each tubing length is identical, lower nozzle arrangement at grade and its interior caliber put in order by it is incremented by successively.During imbibition, the lower port of suction pipe is immersed under the liquid level of fluid sample to be drawn, and control assembly hoisting piston, each suction pipe is filled fluid sample.During tapping, control assembly falls piston, and the fluid sample in suction pipe is added to sample-adding object.Since the identical interior caliber of each tubing length is incremented by successively, when it is filled fluid sample, the fluid sample volume that each suction pipe is drawn is also incremented by successively, thereby realizes the fast and accurately graded doses sample-adding to multiple objects.
Description
Technical field
The present invention relates to liquid sample adding device field more particularly to a kind of automatic ration gradient sample adding devices.
Background technology
When carrying out biochemical analysis test or experiment, pipettor can very easily help laboratory technician to carry out determining for fluid sample
Amount is drawn and sample-adding.When needing to carry out batch sample-adding, sample-adding object on rack for test tube or pallet is arranged, is moved using multiple tracks
Liquid device can quickly finish batch sample-adding.If it is more to be loaded object, sample-adding object is arranged on rack for test tube or pallet
Sample-adding work can be completed using the multiple batches of liquid relief of multichannel pipettor in rectangle.But existing multichannel pipettor is in sample-adding
Isodose sample-adding can only be carried out to multiple sample-adding objects, when needing to carry out graded doses sample-adding to multiple sample-adding objects, just
Quick batch sample-adding cannot be carried out using multichannel pipettor, and each sample-adding object can only be individually loaded, operation is numerous
Trivial, heavy workload, work efficiency is low, and in the gradient sample-adding of multifrequency time, sample-adding operation manually is also easily made mistakes.
The content of the invention
In order to overcome the problems, such as that existing multichannel pipettor cannot carry out gradient sample-adding and in the gradient sample-adding of multifrequency time
Sample-adding operation easily amiss problem manually, the present invention provides a kind of automatic ration gradient sample adding device, automatic ration ladders
Spending the suction nozzle of sample adding device includes suction pipe and casing, and casing is sleeved on the sleeve of pipettor piston component lower end, and suction pipe is vertically worn
It crosses the bottom surface of casing and is fixed on bottom surface, the upper port of suction pipe is higher than the bottom surface and the not bottom surface with sleeve of casing
Connect.The suction pipe of each suction nozzle is arranged in a row suction pipe array in pipettor lower end, and the lower port of each suction pipe of suction pipe array is in same water
In plane and height is identical, and the interior caliber of each suction pipe is incremented by successively in putting in order in suction pipe array by it.When need into
When row gradient is loaded, object first is loaded by dosage escalation arrangement of gradients to be loaded on rack for test tube or sample disc by each, use
The single gradient multichannel pipettor imbibition, after fluid sample is full of each suction pipe, the fluid sample having more is spilt into casing.Tapping
When, only the fluid sample in suction pipe is added to sample-adding object, since the liquid absorption amount of each suction pipe is fixed gradient, thus realizes
The graded doses of each sample-adding object are loaded.
Technical scheme is as follows：
A kind of automatic ration gradient sample adding device, including control assembly, piston component and multiple suction nozzles, control assembly lower end
Piston component is connected, the bottom of piston component is the horizontally arranged sleeve identical with suction nozzle quantity.Each suction nozzle is by 1 casing
With 1 cylindrical suction pipe composition, the casing of suction nozzle is respectively fitted on sleeve.Each suction pipe is respectively perpendicular the bottom surface through casing
And it is fixed on the bottom surface.The upper port of each suction pipe be above the bottom surface of the casing where it and not with sleeve thereon
Bottom surface connects.Each suction pipe is arranged in a row suction pipe array, the height of each suction pipe is identical and its lower port at grade, it is each to inhale
The interior caliber of pipe is incremented by successively by its putting in order in suction pipe array.
Further, control assembly includes PLC controller, stepper motor, gear set, rack, T-shaped connecting rod, gear set
Including driving gear and driven gear, T-shaped connecting rod includes vertical bar and horizon bar.PLC controller is connected with stepper motor, main
Moving gear is fixed on the rotor bearing of stepper motor, and driving gear is engaged with the driven gear, and passive tooth wheel and rack is nibbled
It closes.Rack is fixed on the upper end of the vertical bar of T-bar, and horizon bar is then connected with the upper end of piston rod.
Further, the number of each suction pipe is n in above-mentioned suction pipe array, and the interior caliber ratio of n suction pipe is followed successively by：N is natural number and n >=2.
Further, the suction pipe number in suction pipe array is 8, and the height of 8 suction pipes is 8.82mm, in suction pipe array
First suction pipe interior caliber be 0.38mm.Further, the suction pipe number in suction pipe array is 6, the height of 6 suction pipes
It is 5.10mm, the interior caliber of first suction pipe in suction pipe array is 0.5mm.
Further, the suction pipe number in suction pipe array is 6, and the height of 6 suction pipes is 5.10mm, in suction pipe array
First suction pipe interior caliber be 0.5mm.
Compared with prior art, beneficial effects of the present invention：
A kind of automatic ration gradient sample adding device provided by the invention, what identical by height and interior caliber arranged in gradient
The fluid sample of graded doses is inhaled/put to suction pipe, can once complete to be loaded the graded doses for being loaded object, eliminate existing gradient
Troublesome operation when dosage is loaded；It can also be controlled and lived by PLC controller when needing to carry out the graded doses sample-adding of multifrequency time
The fluid sample of graded doses is inhaled/put in the lifting of plug, efficiently, is accurately carried out graded doses sample-adding to multiple sample-adding objects, is avoided
Human error caused by manual gradient sample-adding.
Description of the drawings
Fig. 1 is a kind of structure diagram of 8 passage automatic ration gradient sample adding device described in the embodiment of the present invention 1；
Fig. 2 is the structure diagram of the piston group described in the embodiment of the present invention 1；
Fig. 3 is the structure diagram of the suction nozzle described in the embodiment of the present invention 1.
In figure 1 above-Fig. 3,1 component in order to control, 2 be piston component, and 3 be sleeve, and 4 be suction nozzle, and 101 be PLC controller,
102 be stepper motor, and 103 be driven gear, and 104 be rack, and 105 be T-shaped connecting rod, and 201 be piston cylinder, 202 be piston,
203 be piston rod, and 401 be casing, and 402 be suction pipe.
Specific embodiment：
The present invention is described further with reference to the accompanying drawings.
Embodiment 1
As shown in Figure 1, a kind of 8 passage automatic ration gradient sample adding devices, including 1 control assembly, 1,1 group of piston component
2,8 sleeves, 3,8 suction nozzles 4, control assembly 1 include 1 102,1 groups of the stepper motor of PLC controller 101,1 gear set, 1
104,1 T-shaped connecting rods 105 of rack.Gear set includes 1 driving gear and 1 driven gear 103, T-shaped connecting rod 105 are wrapped
1 montant and 1 horizon bar are included, PLC controller is connected with stepper motor, and driving tooth is fixed on the rotor bearing of stepper motor
Wheel, driving gear are engaged with driven gear 103, and driven gear 103 is engaged with rack 104, and rack is mounted in T-shaped connecting rod 105
The upper end of vertical bar, the horizon bar of T-shaped connecting rod are connected with the upper end of piston component 2, there is 8 passages in piston component 2,8
Passage is arranged in a row, and 1 piston group is housed in 1 passage.
As shown in Fig. 2, 1 piston group includes 1 202,1, piston, 203,1, piston rod piston cylinder 201, piston rod 203
Lower end be connected with piston 202, the upper end of piston rod 203 is connected with the horizon bar of T-shaped connecting rod 105, the lower end of piston cylinder 201
It is open and is socketed with sleeve 3, sleeve 3 passes through the bottom surface of piston component 2.
As shown in figure 3, suction nozzle 4 includes casing 401 and cylindrical suction pipe 402, casing 401 is sleeved on sleeve 3.Suction pipe
402 pass perpendicularly through the bottom surface of casing 401 and fix on the bottom surface, and the upper port of suction pipe 402 is higher than the bottom surface of casing 401
And the bottom surface with sleeve 3 does not connect.8 suction pipes line up a suction pipe array, and the lower ports of 8 suction pipes 402 is arranged in same
On horizontal plane and its height is 8.82mm, and the interior calibers of 8 suction pipes 402 puts in order incremented by successively by it.8 suction pipes 402
The ratio of interior caliber beThe interior caliber of wherein first suction pipe is 0.38mm, 8 suction pipes 402
Suction/liquid discharge amount is followed successively by 1 microlitre, 2 microlitres, 3 microlitres ... 8 microlitres.
During sample-adding, first object to be loaded is arranged in a row by the sequence of pre- sample-adding dosage escalation.The lower port of suction nozzle 4 is soaked
Under the liquid level of fluid sample to be drawn, PLC controller 101 controls stepper motor 102 to rotate forward, 102 rotor bearing of stepper motor
On driving gear rotated with rotor and driven gear 103 driven to rotate, driven gear 103 rises with carry-over bar 104, T-shaped connect
Extension bar 105 drives 8 piston rods 203 to move upwards predetermined distance in each piston cylinder 201, and each piston rod 203 drives each work
Plug 202 moves upwards identical distance, and each piston forms negative pressure in the piston cylinder below 202, fluid sample is pressed into 8 by atmospheric pressure
A suction nozzle 4.The gradient sample adding device is moved on to above sample-adding object, the fluid sample dosage sequence in suction nozzle 4 and sample-adding object
Dosage sequence to be added corresponds.PLC controller 101 controls stepper motor 102 to invert, and T-shaped connecting rod 105 declines, piston rod
203 move downward in piston cylinder 201, and piston rod 203 is moved downward with piston 202, during 104 involution original position of rack, 8 suctions
Fluid sample in pipe 402 is added into sample-adding object.Due to 8 suction pipes 402 inhale/tapping body sample dose is 1 to 8 microlitres of ladder
Degree thereby realizes and 1 to 8 microlitres of graded doses for being loaded object is loaded.It is high when needing to carry out multiple sample-adding objects
During the graded doses sample-adding of the frequency, the lifting for setting the control programme-control piston 202 of PLC controller 101 achieves that pair
The sample-adding of multiple batches of graded doses rapidly and efficiently of multiple sample-adding objects.
Embodiment 2
On the basis of embodiment 1, the number of suction nozzle 4 is kept to 6, the interior caliber of 6 suction pipes 402 is highly
5.10mm, the interior caliber ratio of 6 suction pipes 402 areThe interior caliber of wherein first suction pipe is
0.5mm.Suction/liquid discharge amount of 6 suction pipes 402 is followed successively by 1 microlitre, 2 microlitres, 3 microlitres ... 6 microlitres.
Claims (5)
1. a kind of automatic ration gradient sample adding device, described including 1 control assembly, 1 piston component and 2 or more suction nozzles
Control assembly lower end connects the piston component, and the bottom of the piston component is horizontally arranged identical with the suction nozzle quantity
Sleeve, which is characterized in that described 2 or more suction nozzles respectively include 1 casing and 1 cylindrical suction pipe, 2 or more casings point
It is not sleeved on the sleeve, 2 or more suction pipes are respectively perpendicular the bottom surface through 2 or more casings and are fixed on the bottom surface
On, the upper ports of described 2 or more suction pipes is above the bottom surface of the casing where it and not the bottom surface phase with the sleeve
Connect, the height of described 2 or more suction pipes is identical and its lower port at grade, described 2 or more suction pipes are arranged in a row suction
Pipe array, the interior caliber of each suction pipe in the suction pipe array are incremented by successively by its putting in order in the suction pipe array.
2. a kind of automatic ration gradient sample adding device according to claim 1, which is characterized in that the control assembly includes
1 PLC controller, 1 stepper motor, 1 gear set, 1 rack, 1 T-shaped connecting rod, 1 gear set include 1
Driving gear and 1 driven gear, 1 T-shaped connecting rod include 1 vertical bar and 1 horizon bar, the PLC controller
Be connected with the stepper motor, be fixed with driving gear on the rotor bearing of the stepper motor, the driving gear with it is described
Driven gear engages, and the driven gear is engaged with the rack, and the rack is fixed on the upper end of the vertical bar, the water
Flat bar is connected with the upper end of the piston rod.
A kind of 3. automatic ration gradient sample adding device according to claim 1 or 2, which is characterized in that the suction pipe array
In each suction pipe number for n, the interior caliber ratio of n suction pipe is followed successively by：N for natural number and
n≥2。
4. a kind of automatic ration gradient sample adding device according to claim 3, which is characterized in that in the suction pipe array
Suction pipe number is 8, and the height of 8 suction pipes is 8.82mm, and the interior caliber of first suction pipe in the suction pipe array is
0.38mm。
5. a kind of automatic ration gradient sample adding device according to claim 3, which is characterized in that in the suction pipe array
Suction pipe number is 6, and the height of 6 suction pipes is 5.10mm, and the interior caliber of first suction pipe in the suction pipe array is
0.5mm。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810014825.7A CN108097341A (en) | 2018-01-08 | 2018-01-08 | A kind of automatic ration gradient sample adding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810014825.7A CN108097341A (en) | 2018-01-08 | 2018-01-08 | A kind of automatic ration gradient sample adding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108097341A true CN108097341A (en) | 2018-06-01 |
Family
ID=62218390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810014825.7A Pending CN108097341A (en) | 2018-01-08 | 2018-01-08 | A kind of automatic ration gradient sample adding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108097341A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608842A (en) * | 2020-12-17 | 2021-04-06 | 清华大学 | Cell hydrogel three-dimensional culture device based on microsatellite |
| CN112881729A (en) * | 2021-01-15 | 2021-06-01 | 中山大学 | Drug concentration gradient generation and sample adding device and application thereof |
| CN113277458A (en) * | 2021-05-24 | 2021-08-20 | 安徽泽升科技有限公司 | Intelligent subpackaging system and method for multiple chemical reagents |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101279297A (en) * | 2008-04-22 | 2008-10-08 | 江苏大学 | Multichannel electronic liquor-transferring system with standard signal interface |
| CN201744362U (en) * | 2009-03-21 | 2011-02-16 | 吴利玲 | Coarse-suction fine-dripping acid and alkali burette |
| US8029742B2 (en) * | 2008-05-05 | 2011-10-04 | Integra Biosciences Corp. | Multi-channel pipettor with repositionable tips |
| CN202290080U (en) * | 2011-05-19 | 2012-07-04 | 东北农业大学 | Combined liquid transfer pipe |
| CN102539806A (en) * | 2012-02-16 | 2012-07-04 | 哈尔滨工业大学（威海） | Multi-channel liquid sampling equivalent distancing device |
| CN103008039A (en) * | 2011-09-20 | 2013-04-03 | 西比欧公司 | Pipetting device with pipette head with a number of pipette channels arranged in a pattern |
| CN103990504A (en) * | 2014-05-27 | 2014-08-20 | 广州冠科生物科技有限公司 | Full-automatic liquid transfer device |
| CN204522997U (en) * | 2014-12-23 | 2015-08-05 | 马荣华 | Quantitative pipette |
| CN104918703A (en) * | 2012-12-28 | 2015-09-16 | 阿赛斯生物股份有限公司 | Pipette |
| CN205613441U (en) * | 2016-04-18 | 2016-10-05 | 神华集团有限责任公司 | Pipette |
| CN206622126U (en) * | 2017-04-13 | 2017-11-10 | 重庆生科源生物技术有限公司 | A kind of adjustable pipette |
| CN206731148U (en) * | 2017-04-10 | 2017-12-12 | 浙江海洋大学 | A kind of micro sample adding appliance for preventing mucus |
| CN206848292U (en) * | 2017-04-25 | 2018-01-05 | 厦门莱蔓新材料科技有限公司 | A kind of multichannel precision is loaded syringe pump module |
-
2018
- 2018-01-08 CN CN201810014825.7A patent/CN108097341A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101279297A (en) * | 2008-04-22 | 2008-10-08 | 江苏大学 | Multichannel electronic liquor-transferring system with standard signal interface |
| US8029742B2 (en) * | 2008-05-05 | 2011-10-04 | Integra Biosciences Corp. | Multi-channel pipettor with repositionable tips |
| CN201744362U (en) * | 2009-03-21 | 2011-02-16 | 吴利玲 | Coarse-suction fine-dripping acid and alkali burette |
| CN202290080U (en) * | 2011-05-19 | 2012-07-04 | 东北农业大学 | Combined liquid transfer pipe |
| CN103008039A (en) * | 2011-09-20 | 2013-04-03 | 西比欧公司 | Pipetting device with pipette head with a number of pipette channels arranged in a pattern |
| CN102539806A (en) * | 2012-02-16 | 2012-07-04 | 哈尔滨工业大学（威海） | Multi-channel liquid sampling equivalent distancing device |
| CN104918703A (en) * | 2012-12-28 | 2015-09-16 | 阿赛斯生物股份有限公司 | Pipette |
| CN103990504A (en) * | 2014-05-27 | 2014-08-20 | 广州冠科生物科技有限公司 | Full-automatic liquid transfer device |
| CN204522997U (en) * | 2014-12-23 | 2015-08-05 | 马荣华 | Quantitative pipette |
| CN205613441U (en) * | 2016-04-18 | 2016-10-05 | 神华集团有限责任公司 | Pipette |
| CN206731148U (en) * | 2017-04-10 | 2017-12-12 | 浙江海洋大学 | A kind of micro sample adding appliance for preventing mucus |
| CN206622126U (en) * | 2017-04-13 | 2017-11-10 | 重庆生科源生物技术有限公司 | A kind of adjustable pipette |
| CN206848292U (en) * | 2017-04-25 | 2018-01-05 | 厦门莱蔓新材料科技有限公司 | A kind of multichannel precision is loaded syringe pump module |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112608842A (en) * | 2020-12-17 | 2021-04-06 | 清华大学 | Cell hydrogel three-dimensional culture device based on microsatellite |
| CN112881729A (en) * | 2021-01-15 | 2021-06-01 | 中山大学 | Drug concentration gradient generation and sample adding device and application thereof |
| CN113277458A (en) * | 2021-05-24 | 2021-08-20 | 安徽泽升科技有限公司 | Intelligent subpackaging system and method for multiple chemical reagents |
| CN113277458B (en) * | 2021-05-24 | 2023-01-13 | 安徽泽升科技有限公司 | Intelligent subpackaging system and method for multiple chemical reagents |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108097341A (en) | A kind of automatic ration gradient sample adding device | |
| CN106442075B (en) | A kind of fully-automatic liquid make-up liquid changing device | |
| WO2006045229A1 (en) | A micro-volume liquid ejection system | |
| CN107462734B (en) | Sample adding device applied to liquid biological reaction system and control method | |
| CN207133187U (en) | Dietetic food detection device | |
| JP2016511001A5 (en) | ||
| CN108176419A (en) | A kind of automatic gradient sample adding device | |
| WO2019174471A1 (en) | Pipetting mechanism | |
| US3484207A (en) | Liquid sampling-pipetting method and apparatus | |
| CN104062170A (en) | Automatic online micro-scale sample dilution and mixing system and mixing method | |
| CN207123539U (en) | The dilution wiper mechanism of dietetic food detection device | |
| US20100247775A1 (en) | Precise Fluid Dispensing Method and Device | |
| EP0081919A1 (en) | Test fluid preparation apparatus | |
| US3401564A (en) | Apparatus for transferring measured samples | |
| CN218872250U (en) | Micro-fluidic chip reagent filling instrument | |
| JP7372231B2 (en) | How to identify resin slurry for chromatography | |
| CN111289341A (en) | Automatic sample introduction diluting device and method thereof | |
| CN211800915U (en) | High-precision trace liquid filling equipment | |
| CN209493053U (en) | A kind of automatic single channel liquid shifting equipment | |
| CN110505920B (en) | Method and metering device for contact metering of liquids | |
| CN103267551A (en) | Precise measuring device with changeable volume | |
| CN109060628B (en) | Variable head permeation experiment device capable of accurately and rapidly measuring permeability coefficient of soil engineering sample | |
| RU2488801C2 (en) | Method of collecting and diluting portions of radioactive solution and apparatus for realising said method (versions) | |
| CN108273577A (en) | A kind of single gradient multichannel pipettor | |
| CN103776854B (en) | A kind of solution sample creep experimental system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180601 |