CN103558074A - Cell-stain apparatus - Google Patents
Cell-stain apparatus Download PDFInfo
- Publication number
- CN103558074A CN103558074A CN201310470832.5A CN201310470832A CN103558074A CN 103558074 A CN103558074 A CN 103558074A CN 201310470832 A CN201310470832 A CN 201310470832A CN 103558074 A CN103558074 A CN 103558074A
- Authority
- CN
- China
- Prior art keywords
- frame
- cell
- transfer pipet
- rack
- driving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a cell-stain apparatus, comprising a rack; a plurality of staining plates, each staining plate being detachably provided with a plurality of staining bins; a conveying assembly which is installed at the lower part of the rack, is used for driving the plurality of staining plates and moves in a length direction of the rack; two reagent shelves which are movably installed above the rack and disposed above the conveying assembly; two driving units which drive the two reagent shelves to move in the length direction of the rack; an installation frame movably installed on the rack; the bottom of the installation rack is provided with a plurality of pipettes arranged along the width direction of the rack; the upper openings of the pipettes are communicated with an air pump installed on the rack through soft pipes; and a drive assembly for driving the installation rack up and down along the height direction of the rack. Compared with conventional apparatuses, the cell-stain apparatus provided by the invention is in no need of human hands, can effectively reduce pollution probability of cell samples and staining agents, can increase efficiency of cell stains, and is convenient for operation and small in space occupation.
Description
Technical field
The present invention relates to cell dyeing technical field, be specifically related to a kind of cell-stain apparatus.
Background technology
In medical field, normally, to after histocyte dyeing, it is carried out to the detection of disease.Present stage, normally adopt special-purpose cell-stain apparatus to complete the dyeing course of cell.
The automaticity of existing cell-stain apparatus is lower, it is in operating process, a lot of steps all need to rely on people's manual manipulation, affected the efficiency of cell dyeing, and staff is in operating process, the possibility contacting with cell sample, coloring agent etc. is larger, thereby increased the contaminated probabilities such as cell sample and coloring agent, finally caused the detection in later stage and actual conditions to have deviation.
Summary of the invention
For the deficiencies in the prior art, object of the present invention is intended to provide a kind of cell-stain apparatus, and its automaticity is higher, avoids people's manual manipulation completely, thereby improves the efficiency of cell dyeing, reduces the contaminated probabilities such as cell sample, coloring agent.
For achieving the above object, the present invention adopts following technical scheme:
Cell-stain apparatus, comprises,
Frame;
The dyed plate that a plurality of bearing of trends are consistent with the Width of frame, equal dismountable a plurality of dyeing storehouse that is provided with on each dyed plate, arrange along the length direction of dyed plate in the plurality of dyeing storehouse;
Be arranged on frame lower for driving a plurality of dyed plates at the conveying assembly of the length direction motion of frame;
Movable two reagent rack that are arranged in frame and are positioned at conveying assembly top, these two reagent rack are respectively used to place histiocytic sample hose and coloring agent are housed, and all have the position, hole of getting liquid rifle head for placing on its two;
Drive respectively two reagent rack at two driver elements of the length direction motion of frame;
Be movably arranged on the erecting frame in frame, the bottom of this erecting frame is provided with a plurality of transfer pipets of arranging along frame Width, and the upper end open of transfer pipet is communicated with rack-mounted air pump by flexible pipe;
For driving erecting frame along the up and down driven unit of the short transverse of frame.
Conveying assembly comprises,
Be articulated in the main drive shaft of frame length direction one end, main drive shaft extends along the Width of frame, and its two ends are synchronously connected with respectively a drive sprocket;
Be articulated in the driven shaft of the frame length direction other end, driven shaft extends along the Width of frame, and its two ends are synchronously connected with respectively a driven sprocket;
For the first motor that drives main drive shaft to rotate;
Two driving chains, it is outside at drive sprocket and the driven sprocket of the same side that this two driving chain is distinguished synchronous winding; Driving chain is comprised of a plurality of chain links, between two adjacent chain links, be provided with a connection piece, the two ends of web member are hinged with the chain link that is positioned at its two ends respectively, are fixed with swivel base on web member, and the two ends of dyed plate are articulated in respectively two swivel bases relative on two driving chains.
The two ends of dyed plate are respectively arranged with an installing plate, the inner side, bottom of installing plate is fixed in the end of dyed plate, the top pars intermedia of two installing plates is provided with outward extending pivot, the center line of two pivots overlaps, and two pivots are installed on two swivel bases relative on two driving chains by bearing respectively.
The front end of frame is provided with a photoelectric sensor, and a shadow shield corresponding with this photoelectric sensor is installed on one of them driving chain.
The both sides of frame are respectively arranged with a guide rail extending along frame length direction, and what slided respectively in the both sides of two reagent rack is arranged on two guide rails; Driver element comprises second motor of body partial fixing in frame, be articulated in frame length direction one end synchronizes the driving pulley connecting, the driven pulley that is articulated in the frame length direction other end, synchronous winding at the synchronous belt of driving pulley and driven pulley outside with the second motor, two reagent rack are fixedly connected on respectively on the synchronous belt of two driver elements.
This cell-stain apparatus also includes a upset liquid pouring machine structure, this upset liquid pouring machine structure comprises, be arranged on dyed plate end gear, the movable orientation that is arranged on the double wedge in frame and on it tooth bar consistent with frame length direction, for carry-over bar one and the gear position of meshing and one and the gear position of mutually staggering between the driving mechanism that moves.
Driving mechanism comprises three motor of body partial fixing in frame, be synchronously connected on the 3rd machine shaft and being socketed in the feed screw nut that transmits on screw mandrel, being arranged in frame and bearing of trend and the consistent line slide rail of frame Width of drive lead screw that bearing of trend is consistent with the Width of frame, coupling, and tooth bar is fixedly connected on being arranged on line slide rail that feed screw nut goes up and slide.
Driven unit comprises four motor of a body partial fixing in frame, and erecting frame connects with the rotating shaft of the 4th motor in the mode of screw rod transmission.
The cross-under of transfer pipet activity also can be movable with respect to erecting frame along the short transverse of frame on erecting frame, the top ends of transfer pipet is provided with the catch for preventing that transfer pipet from moving downward with respect to erecting frame, is provided with the defining flange that is positioned at catch below on transfer pipet; Between defining flange on each transfer pipet and erecting frame, be provided with a spring, this spring is for providing an elastic stress that the transfer pipet corresponding with it is pushed up up and down to pressure.
The bottom of erecting frame is fixed with a plurality of sliding sleeves that equate with transfer pipet quantity, and transfer pipet activity cross-under is one to one in sliding sleeve, and catch is mounted on the top ends of sliding sleeve, and spring is limited between defining flange and the bottom face of sliding sleeve.
The bottom activity of erecting frame a pressing plate is installed, one the 5th motor is installed on erecting frame, the rotating shaft of the 5th motor is connected with pressing plate in the mode of screw rod transmission and for moving up and down with respect to erecting frame in the short transverse of frame with dynamic pressure plate, on pressing plate, be provided with the through hole passing for transfer pipet, the periphery of through hole is formed with and when pressing plate moves downward with respect to erecting frame, pushes up the top splenium of pressing the end, the rifle crown on transfer pipet downwards.
The width of through hole be greater than transfer pipet diameter, be less than the diameter of rifle cephalic par.
Beneficial effect of the present invention is:
Than prior art, cell-stain apparatus of the present invention, without people's manual manipulation, can effectively reduce cell sample, the contaminated probability of coloring agent, and can improve the efficiency of cell dyeing; Meanwhile, it is easy to operate, takes up room less.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is inner structure schematic diagram of the present invention;
Fig. 3 is the structural representation of conveying assembly in Fig. 1;
Fig. 4 is the scheme of installation of conveying assembly in Fig. 1;
Fig. 5 is the structural representation of driving chain in Fig. 3;
Fig. 6 is the overturn structural representation of liquid pouring machine structure of the present invention;
Fig. 7 is the structural representation of driving mechanism in Fig. 6;
Fig. 8 is the working state schematic representation of liquid pouring machine structure of overturning in Fig. 6;
The structural representation of Fig. 9 reagent rack of the present invention;
Figure 10 is the take arms structural representation of a mechanism of the present invention;
Figure 11 is the zoomed-in view at A place in Figure 10;
Figure 12 is the B-B zoomed-in view of Figure 11;
Figure 13 is the structural representation of Tuo Qiangtou of the present invention mechanism;
Figure 14 is the scheme of installation of Figure 13 Zhong Tuoqiangtou mechanism;
Figure 15 is the working state schematic representation of Figure 13 Zhong Tuoqiangtou mechanism;
Wherein: 100, frame; 110, side plate; 120, fixed mount; 121, guide pole; 210, reagent rack; 220, reagent rack; 230, the first guide rail; 240, the second guide rail; 250, driver element; 260, driver element; 261, the second motor; 262, driving pulley; 263, driven pulley; 264, driving belt; 300, conveying assembly; 310, driving chain; 311, chain link; 312, bending sheet; 313, outer brace; 314, interior brace; 315, swivel base; 320, main drive shaft; 321, drive sprocket; 330, driven shaft; 331, driven sprocket; 340, dyed plate; 341, dyeing storehouse; 342, installing plate; 343, pivot; 344, gear; 350, driven unit; 351, the 3rd motor; 352, drive lead screw; 353, feed screw nut; 354, contiguous block; 355, line slideway; 356, tooth bar; 357, web joint; 358, photoelectric sensor; 359, shadow shield; 400, erecting frame; 401, riser; 402, orienting sleeve; 403, crossbeam is installed; 404, sliding sleeve; 405, line slideway; 410, transfer pipet; 411, defining flange; 412, catch; 413, spring; 421, pressing plate; 422, through hole; 423, link; 431, the 4th motor; 432, drive lead screw; 433, feed screw nut; 441, the 5th motor; 442, drive lead screw; 443, feed screw nut; 500, air pump; 60, rifle head.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the present invention is described further:
For cell-stain apparatus of the present invention, it comprises frame 100, a plurality of dyed plate 340, conveying assembly 300, reagent rack 210, reagent rack 220, erecting frame 400, a plurality of transfer pipet 410 and the air pump 500 equating with transfer pipet 410 quantity.In figure, X-direction refers to the length direction of frame 100, and Y-direction refers to the Width of frame 100, and Z-direction refers to the short transverse of frame 100.
The Width of the bearing of trend frame 100 of dyed plate 340 is consistent, conveying assembly 300 is mainly used for the length direction along frame 100 by a plurality of dyed plates 340 and moves, it comprises main drive shaft 320, driven shaft 330, the first motor (not shown) and two driving chains 310, wherein, main drive shaft 320 and driven shaft 330 lay respectively at the two ends of conveying mechanism and parallel to each other, the two ends of main drive shaft 320 respectively key are connected with a drive sprocket 321, the two ends of driven shaft 330 respectively key are connected with a driven sprocket 331, two driving chains 310 respectively synchronous winding in the drive sprocket 321 and driven sprocket 331 outsides that are positioned at the same side, thereby by main drive shaft 320 and the synchronous connection of driven shaft 330.The rotating shaft of the first motor can be to be connected with the direct key of main drive shaft 320, can be also to synchronize connection with main drive shaft 320 with belt gear or gear-driven mode.
The first driven by motor main drive shaft 320 rotates, two driving chains 310 are along with drive sprocket 321 and the synchronous motion of driven sprocket 331, on two driving chains 310, two relative swivel bases 315 are synchronized with the movement, that is to say, the two ends of dyed plate 340 are synchronized with the movement, when dyed plate 340 moves to driving chain 310 turning, owing to thering is certain distance between dyed plate 340 and pivot 343, turning at driving chain 310, dyed plate 340 is under the effect of its suffered gravity, its upper face remains horizontality, that is to say, on dyed plate 340, the opening on fixing 341 tops, dyeing storehouse all the time upward, thereby the reactant liquor in the storehouse 341 that can guarantee to dye can not be poured out.Most importantly, in above-mentioned use procedure, owing to adopting driving chain 310 to drive dyed plate 340 motions, make a plurality of dyed plates 340 along driving chain 310 shuttling movements, therefore can effectively reduce the space that conveying mechanism takies.
It should be noted that, in such scheme, a bending sheet 312 to be set on interior brace 314 for swivel base 315 is installed, in the present invention, also can be on brace 313, to arrange one for the parts of swivel base 315 are installed outside, or the web member that connects two chain links 311 also can adopt other forms, as long as when can meet pivot 343 pivot joint, and do not hinder the proper motion of driving chain 310.
Front end in frame 100 is also provided with a photoelectric sensor, this photoelectric sensor is a kind of conventional correlation photoelectricity, on driving chain 310, be provided with an anti-dazzling screen, this anti-dazzling screen is when moving along with driving chain 310, can be through transmitting terminal and the receiving end of correlation photoelectricity, make correlation photoelectricity to a controller transmitted signal, by controller, control the unlatching of the first motor and stop, making some dyed plates 340 be parked in accurately erecting frame 400 under.
In above scheme, in order to realize the conveying of circulation, can be also to utilize feed belt to replace driving chain 310, only the two ends of dyed plate 340 need to be articulated in respectively on the feed belt of both sides.
The present invention also includes a upset liquid pouring machine structure coordinating with conveying assembly 300, it comprises tooth bar 356, driven unit 350 and gear 344, wherein a plurality of dyed plates 340 are parallel to each other, and equally spaced the arranging of extension track along driving chain 310, the synchronous gear 344 that is connected with on the pivot 343 of dyed plate 340 one end, this gear 344 is for coordinating with tooth bar 356, realize the upset of dyed plate 340, thereby make the dyeing storehouse on dyed plate 340 opening down, pour out the liquid in dyeing storehouse, concrete mode is, be arranged on and the gear 344 of above-mentioned tooth bar 356 activities are positioned on the side plate 110 of the same side, the orientation of the double wedge on this tooth bar 356 is consistent with the length direction of frame 100, driven unit 350 is for the Width motion in frame 100 with carry-over bar 356, between the position that to be driven unit 350 mutually stagger at frame 100 Widths with the position of gear 344 engagements and and gear 344 at carry-over bar 356 below gear 344, move, in the time the liquid in the storehouse 341 of dyeing on some dyed plates 340 need to being poured out, at this dyed plate 340, be transferred assembly 300
Drive to being positioned near before the position of tooth bar 356, start driven unit 350, tooth bar 356 is driven to positions above-mentioned and gear 344 engagements, when dyed plate 340 moves to the position of the liquid that overturns, first gear 344 meshes with tooth bar 356 front ends, at gear 344, along with length direction (be the length direction of tooth bar 356) when motion of dyed plate 340 along frame 100, gear 344 rotates slowly, thereby drives dyed plate 340 to overturn.
Driven unit 350 comprises that body part is fixed on the 3rd motor 351 on side plate 110 by a web joint 357, the drive lead screw 352 that is synchronously connected in the rotating shaft of the 3rd motor 351 and extends along the Width of frame 100, coupling be socketed in the feed screw nut 353 on drive lead screw 352, be arranged on web joint 357 and two line slideways 355 that extend along frame 100 Widths, on feed screw nut 353, be fixed with a contiguous block 354, what slided respectively in the two ends of tooth bar 356 is arranged on two line slideways 355, the top ends of contiguous block 354 is fixedly connected with the pars intermedia of tooth bar 356, start the 3rd motor 351, can be with carry-over bar 356 to move along line slideway 355, the Width along frame 100 moves.
For convenient, control, improve automaticity of the present invention, on web joint 357, be installed with a photoelectric sensor 358 to controller transmitted signal, one shadow shield 359 is installed on contiguous block 354, this shadow shield 359 is along with contiguous block 354 and tooth bar 356 move at the Width of frame 100, concrete is, this shadow shield 359 can move between a transmitting terminal at photoelectric sensor 358 and the position between receiving end and a position separated with photoelectric sensor 358, when shadow shield 359 is between the transmitting terminal of photoelectric sensor 358 and receiving end, the receiving end of photoelectric sensor 358 can not receive the light signal that transmitting terminal sends, now, it is the signal that the 3rd motor 351 is opened that photoelectric sensor 358 sends to the signal of controller, otherwise, when shadow shield 359 is separated with photoelectric sensor 358, the receiving end of photoelectric sensor 358 can receive the light signal that transmitting terminal sends, now, it is the signal that the 3rd motor 351 is stopped that photoelectric sensor 358 sends to the signal of controller.In concrete use procedure, having need to while falling liquid, can allow the 3rd motor 351 with carry-over bar 356, move to the position of meshing with gear 344, this just need above-mentioned controller in normal cell dyeing process in holding state, , on controller, connect one for making controller be transformed into the trigger switch of duty from holding state, when needs overturn while falling liquid, open the moment of trigger switch, controller is started working, due to when the original state, between the transmitting terminal and receiving end of shadow shield 359 in photoelectric sensor 358, open the moment of trigger switch, controller is controlled the 3rd motor 351 and is opened, when tooth bar 356 is during in state with gear 344 engagement, shadow shield 359 is just in time separated with photoelectric sensor 358, controller is controlled the 3rd motor 351 and is stopped.
Above-mentioned driven unit 350 also can be with carry-over bar 356 to move in short transverse, when needs overturn dyed plate 340, driven unit 350 band carry-over bars 356 move upward and gear 344 engagements, while not needing to overturn dyed plate 340,350 band carry-over bars 356 of driven unit move downward separated with gear 344, that is to say, in the present invention, 350 needs of driven unit can with carry-over bar 356 one with the position of gear 344 engagement and a position separated with gear 344 between motion.
In the both sides of frame 100, be separately installed with the first guide rail 230 and the second guide rail 240 that along the length direction of frame 100, extend, what slided respectively in the both sides of reagent rack 210 and reagent rack 220 is arranged on the first guide rail 230 and the second guide rail 240, driver element 250 is arranged in frame 100 for driving reagent rack 210 along the length direction of frame 100 length direction of the first guide rail 230 and the second guide rail 240 (along) motion, driver element 260 is arranged in frame 100 for driving reagent rack 220 along the length direction of frame 100 length direction of the first guide rail 230 and the second guide rail 240 (along) motion.Driver element 250 driver element 260 structures are identical, take driver element 260 as example, it comprises second motor 261 of body partial fixing in frame 100, be positioned at the rearward end of frame 100 and be synchronously connected in the driving pulley 262 in the second motor 261 rotating shafts, be articulated in the driven pulley 263 of frame 100 leading sections, the synchronous driving belt 264 that is set around driving pulley 262 and driven pulley 263 outsides, the side of reagent rack 220 is connected on driving belt 264 by web joint, start the second motor 261, can drive transmission reagent rack 220 to move along the length direction of frame 100.Because the structure of driver element 250 is identical with driver element 260, do not do repeat specification here, the side of reagent rack 210 is connected on the driving belt of driver element 250 by web joint.
In the present invention, driver element 250 and driver element 260 can also be by the lead screw transmission mechanism of driven by motor etc., as long as can drive reagent rack 210 and reagent rack 220 independently to slide along the first guide rail 230 and the second guide rail 240 respectively.Driver element 250 and driver element 260 lay respectively at the outside of the first guide rail 230 and the second guide rail 240, thereby can not interfere reagent rack 210 and reagent rack 220 along frame 100 length direction to-and-fro movements.Can utilize reagent rack 210 to place sample hoses and for the rifle head of sample thief, utilize reagent rack 220 to place coloring agents and for getting the rifle head of coloring agent, reagent rack 210 and reagent rack 220 are independently moved, can not interfere mutually, and its two shared the first guide rail 230 and second guide rail 240, facilitated when using, simplified the structure of dyeing apparatus.
A plurality of transfer pipet 410 of the present invention is arranged on the bottom of erecting frame 400, and the equally spaced width along frame 100 forms a line, wherein, erecting frame 400 is movably arranged on the fixed mount 120 in frame 100, the installation crossbeam 403 that it comprises riser 401 and is fixed on riser 401 bottoms, wherein, the both sides of riser 401 are installed with respectively orienting sleeve 402, on the guide pole 121 that is socketed in fixed mount 120 both sides that the orienting sleeve 402 of both sides slides respectively, guide pole 121 extends along the short transverse of frame 100, be that erecting frame 400 can move up and down with respect to fixed mount 120 along the short transverse of frame 100, above-mentioned a plurality of transfer pipets 410 are installed in to be installed on crossbeam 403, and be arranged in row along the length direction that crossbeam 403 is installed, certainly, the plurality of transfer pipet 410 can be to be also arranged on and to install on crossbeam 403 with other arrangement mode, if can with reagent rack 210 and reagent rack 220 on to put the position, hole of rifle head unanimous between the higher and lower levels.Install on crossbeam 403 and be fixed with a plurality of sliding sleeves 404 that equate with transfer pipet 410 quantity, the hole of sliding sleeve 404 inside axially consistent with short transverse, transfer pipet 410 slides cross-under one to one in sliding sleeve 404, the top ends of transfer pipet 410 is provided with a catch 412, this catch 412 is positioned at sliding sleeve 404 top ends tops, it is for being articulated in sliding sleeve 404 by transfer pipet 410, prevent transfer pipet 410 and move downward with respect to crossbeam 403 is installed, avoid transfer pipet 410 to come off from crossbeam 403 is installed, on transfer pipet 410, be also provided with a defining flange 411 that is positioned at catch 412 belows, transfer pipet 410 outer cover are connected to a spring 413, this spring 413 is limited between defining flange 411 and the bottom face of sliding sleeve 404, pressurized under these spring 413 normalities, it can provide one by transfer pipet 410 elastic stress that press on top downwards.Moving up and down of erecting frame 400 driven by one the 4th motor 431, concrete is, the body partial fixing of the 4th motor 431 is on fixed mount 120, in the rotating shaft of the 4th motor 431, be synchronously connected with the drive lead screw 432 that a short transverse along frame 100 is extended, on drive lead screw 432, coupling is socketed with a feed screw nut 433, riser 401 is fixedly connected on this feed screw nut 433, start the 4th motor 431, can drive erecting frame 400 to move up and down with respect to frame 100 along short transverse, drive above-mentioned a plurality of transfer pipets 410 to move downward to take arms the action of head.
Open the 4th motor 431, drive transfer pipet 410 towards the rifle headstock, to move downwards, transfer pipet 410 is inserted by the upper opening of rifle head, when transfer pipet 410 inserts after a segment distance, the resistance that rifle head applies transfer pipet 410 is increasing, transfer pipet 410 starts to move upward with respect to crossbeam 403 is installed, simultaneously, the elastic stress that spring 413 is applied on transfer pipet 410 increases gradually, in the process of above-mentioned transfer pipet 410 and rifle head plug-in mounting, buffer action due to spring 413, for the different rifle head of openings of sizes, there is small difference in transfer pipet 410 downwards actual mobile distance, concrete is, for the larger rifle head of opening, the actual distance moving down of the transfer pipet 410 corresponding with it is larger, and for the less rifle head of opening, the actual distance moving down of the transfer pipet 410 corresponding with it is less, finally, when being installed, crossbeam 403 moves down after certain distance, can guarantee that all transfer pipets 410 all coordinate with rifle head is gapless.
It should be noted that, also can be without on installation crossbeam 403, sliding sleeve 404 being set in above-mentioned scheme, can be directly on crossbeam 403, to offer a plurality of quantity and bearing of trend equal with transfer pipet 410 and the consistent through hole of short transverse installing, the cross-under of transfer pipet 410 activities is in through hole, in order to prevent that transfer pipet 410 from beat occurring when with rifle head plug-in mounting, can increase the thickness that crossbeam 403 is installed, the through hole on it is had compared with big-length.
In addition, for after moving liquid and completing, realize coming off of rifle head 60, one pressing plate 421 is installed on riser 401, concrete, pressing plate 421 be positioned at install crossbeam 403 under, in side direction, bending forms a link 423 thereafter, what link 423 slided is arranged on the line slideway 405 extending along frame 100 short transverses that is positioned at riser 401 back sides, and pressing plate 421 line slideway 405 of can postponing slides up and down with respect to erecting frame 400;
On pressing plate 421, be provided with a plurality of through holes 422, the plurality of through hole 422 is corresponding one by one with transfer pipet 410, transfer pipet 410 is connected in the through hole corresponding with it 422, the width of this through hole 422 is slightly larger than the diameter of transfer pipet 410, when avoiding pressing plate 421 with respect to erecting frame 400 motion, transfer pipet 410 and pressing plate 421 collisions, the width of through hole 422 is slightly less than the diameter of rifle head 60 top ends, that is to say, rifle head 60 can not be passed by through hole 422, the periphery of through hole 422 forms one for pushing up the top splenium of pressing rifle head 60 top ends, rifle head 60 is housed on transfer pipet 410 and when pressing plate 421 is during downwards with respect to erecting frame 400 motion, the top splenium of through hole 422 peripheries can push up the top end face of pressing rifle head 60 downwards, rifle head 60 is moved downward with transfer pipet 410 to depart from.Moving up and down of pressing plate 421 is that the 5th motor 441 on riser 401 drives by body partial fixing, in the rotating shaft of the 5th motor 441, be synchronously connected with a drive lead screw 442 extending along frame 100 short transverses, on drive lead screw 442, coupling is socketed with a feed screw nut 443, this feed screw nut 443 is fixedly connected on link 423, start the 5th motor 441, can be with dynamic pressure plate 421 to move up and down with respect to erecting frame 400.
During rifle head 60 on need to taking off transfer pipet 410, open the 5th motor 441, drive pressing plate 421 to move downward, when pressing plate 421 moves to the position contacting with rifle head 60 top ends, the top splenium that is positioned at through hole 422 peripheries on pressing plate 421 starts top downwards and presses rifle head 60, until rifle head 60 departs from completely with transfer pipet 410, afterwards, the 5th motor 441 rotates backward, and band dynamic pressure plate 421 is got back to initial position, transfer pipet 410 60 action of preparing next time to take arms.
The present invention in use, utilize conveying assembly 300 to drive a plurality of dyed plates 340 along the length direction of frame 100 moving intermittently, the reagent rack 210 and the reagent rack 220 that are positioned at conveying assembly 300 tops are respectively used to place sample hose and the coloring agent that cell sample is housed, a plurality of transfer pipets 410 are positioned at the top of reagent rack 210 and reagent rack 220, in the time of under conveying assembly 300 is transported to dyed plate 340 in transfer pipet 410, dyed plate 340 rests on this position, now reagent rack 210 move backward to transfer pipet 410 under, transfer pipet 410 is got back to elemental height move down the head of taking arms in reagent rack 210 after, reagent rack 210 is moving along frame 100 length directions, make transfer pipet 410 just in time be positioned at the top of a stock layout QC, now transfer pipet 410 is got back to elemental height after again moving down and drawing cell sample, reagent rack 210 transverse shiftings, transfer pipet 410 moves down the sample in rifle head is injected into a plurality of dyeing storehouse 341 on the dyed plate 340 being positioned under it, turn down afterwards rifle head, reagent rack 220 moves forward to the below of transfer pipet 410, transfer pipet 410 is taken arms after head in reagent rack 220, from reagent rack 220, draw coloring agent again, reagent rack 220 moves to initial position backward afterwards, transfer pipet 410 drives rifle head coloring agent to be injected in the dyeing storehouse with cell sample, after completing a dyeing storehouse fluid injection on dyed plate 340, conveying assembly 300 drives a plurality of dyed plates 340 to move, make next dyed plate 340 be positioned at transfer pipet 410 belows, repeat above-mentioned action.In above operating process, suction liquid is realized by air pump 500.When all completing fluid injection, whole dyeing storehouses completes, and need to pour out after the liquid in dyeing storehouse, start the 3rd motor 351, tooth bar 356 is moved inward along the Width of frame 100, move to subsequently gear 344 on the dyed plate 340 at tooth bar 356 places all can with tooth bar 356 engagements, liquid is fallen in the upset that realizes dyeing storehouse 341.Without manually, there is higher automaticity above, improved the efficiency of cell dyeing, and the pollution that can avoid staff contact to cause.
To one skilled in the art, can make other various corresponding changes and deformation according to technical scheme described above and design, and within these all changes and deformation all should belong to the protection domain of the claims in the present invention.
Claims (12)
1. cell-stain apparatus, is characterized in that, comprise,
Frame;
The dyed plate that a plurality of bearing of trends are consistent with the Width of frame, equal dismountable a plurality of dyeing storehouse that is provided with on each dyed plate, arrange along the length direction of dyed plate in the plurality of dyeing storehouse;
Be arranged on frame lower for driving a plurality of dyed plates at the conveying assembly of the length direction motion of frame;
Movable two reagent rack that are arranged in frame and are positioned at conveying assembly top, these two reagent rack are respectively used to place histiocytic sample hose and coloring agent are housed, and all have the position, hole of getting liquid rifle head for placing on its two;
Drive respectively two reagent rack at two driver elements of the length direction motion of frame;
Be movably arranged on the erecting frame in frame, the bottom of this erecting frame is provided with a plurality of transfer pipets of arranging along frame Width, and the upper end open of transfer pipet is communicated with rack-mounted air pump by flexible pipe;
For driving erecting frame along the up and down driven unit of the short transverse of frame.
2. cell-stain apparatus as claimed in claim 1, is characterized in that, conveying assembly comprises,
Be articulated in the main drive shaft of frame length direction one end, main drive shaft extends along the Width of frame, and its two ends are synchronously connected with respectively a drive sprocket;
Be articulated in the driven shaft of the frame length direction other end, driven shaft extends along the Width of frame, and its two ends are synchronously connected with respectively a driven sprocket;
For the first motor that drives main drive shaft to rotate;
Two driving chains, it is outside at drive sprocket and the driven sprocket of the same side that this two driving chain is distinguished synchronous winding; Driving chain is comprised of a plurality of chain links, between two adjacent chain links, be provided with a connection piece, the two ends of web member are hinged with the chain link that is positioned at its two ends respectively, are fixed with swivel base on web member, and the two ends of dyed plate are articulated in respectively two swivel bases relative on two driving chains.
3. cell-stain apparatus as claimed in claim 2, it is characterized in that, the two ends of dyed plate are respectively arranged with an installing plate, the inner side, bottom of installing plate is fixed in the end of dyed plate, the top pars intermedia of two installing plates is provided with outward extending pivot, the center line of two pivots overlaps, and two pivots are installed on two swivel bases relative on two driving chains by bearing respectively.
4. cell-stain apparatus as claimed in claim 2, is characterized in that, the front end of frame is provided with a photoelectric sensor, and a shadow shield corresponding with this photoelectric sensor is installed on one of them driving chain.
5. cell-stain apparatus as claimed in claim 1, is characterized in that, the both sides of frame are respectively arranged with a guide rail extending along frame length direction, and what slided respectively in the both sides of two reagent rack is arranged on two guide rails; Driver element comprises second motor of body partial fixing in frame, be articulated in frame length direction one end synchronizes the driving pulley connecting, the driven pulley that is articulated in the frame length direction other end, synchronous winding at the synchronous belt of driving pulley and driven pulley outside with the second motor, two reagent rack are fixedly connected on respectively on the synchronous belt of two driver elements.
6. cell-stain apparatus as claimed in claim 1, it is characterized in that, this cell-stain apparatus also includes a upset liquid pouring machine structure, this upset liquid pouring machine structure comprises, be arranged on dyed plate end gear, the movable orientation that is arranged on the double wedge in frame and on it tooth bar consistent with frame length direction, for carry-over bar one and the gear position of meshing and one and the gear position of mutually staggering between the driving mechanism that moves.
7. cell-stain apparatus as claimed in claim 6, it is characterized in that, driving mechanism comprises three motor of body partial fixing in frame, be synchronously connected on the 3rd machine shaft and being socketed in the feed screw nut that transmits on screw mandrel, being arranged in frame and bearing of trend and the consistent line slide rail of frame Width of drive lead screw that bearing of trend is consistent with the Width of frame, coupling, and tooth bar is fixedly connected on being arranged on line slide rail that feed screw nut goes up and slide.
8. cell-stain apparatus as claimed in claim 1, is characterized in that, driven unit comprises four motor of a body partial fixing in frame, and erecting frame connects with the rotating shaft of the 4th motor in the mode of screw rod transmission.
9. cell-stain apparatus as claimed in claim 8, it is characterized in that, the cross-under of transfer pipet activity also can be movable with respect to erecting frame along the short transverse of frame on erecting frame, the top ends of transfer pipet is provided with the catch for preventing that transfer pipet from moving downward with respect to erecting frame, is provided with the defining flange that is positioned at catch below on transfer pipet; Between defining flange on each transfer pipet and erecting frame, be provided with a spring, this spring is for providing an elastic stress that the transfer pipet corresponding with it is pushed up up and down to pressure.
10. cell-stain apparatus as claimed in claim 9, it is characterized in that, the bottom of erecting frame is fixed with a plurality of sliding sleeves that equate with transfer pipet quantity, transfer pipet activity cross-under is one to one in sliding sleeve, catch is mounted on the top ends of sliding sleeve, and spring is limited between defining flange and the bottom face of sliding sleeve.
11. cell-stain apparatuses as claimed in claim 1, it is characterized in that, the bottom activity of erecting frame a pressing plate is installed, one the 5th motor is installed on erecting frame, the rotating shaft of the 5th motor is connected with pressing plate in the mode of screw rod transmission and for moving up and down with respect to erecting frame in the short transverse of frame with dynamic pressure plate, on pressing plate, be provided with the through hole passing for transfer pipet, the periphery of through hole is formed with and when pressing plate moves downward with respect to erecting frame, pushes up the top splenium of pressing the end, the rifle crown on transfer pipet downwards.
12. cell-stain apparatuses as claimed in claim 11, is characterized in that, the width of through hole is greater than the diameter of transfer pipet, is less than the diameter of rifle cephalic par.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310470832.5A CN103558074B (en) | 2013-10-10 | 2013-10-10 | Cell-stain apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310470832.5A CN103558074B (en) | 2013-10-10 | 2013-10-10 | Cell-stain apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103558074A true CN103558074A (en) | 2014-02-05 |
| CN103558074B CN103558074B (en) | 2016-04-13 |
Family
ID=50012384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310470832.5A Active CN103558074B (en) | 2013-10-10 | 2013-10-10 | Cell-stain apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103558074B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897980A (en) * | 2014-02-26 | 2014-07-02 | 北京中纳海润生物科技有限公司 | Liquid moving gun inserting device for biologic molecule purification and separation |
| CN107760561A (en) * | 2017-10-26 | 2018-03-06 | 深圳市汇研科创生物科技有限公司 | Nucleic acid-extracting apparatus and its application method |
| CN109297794A (en) * | 2018-10-18 | 2019-02-01 | 广州安必平医药科技股份有限公司 | All-in-one machine is dyed in dyed plate and film-making |
| CN109297792A (en) * | 2018-10-18 | 2019-02-01 | 广州安必平医药科技股份有限公司 | All-in-one machine is dyed in film-making |
| CN110270389A (en) * | 2019-07-22 | 2019-09-24 | 江苏集萃微纳自动化系统与装备技术研究所有限公司 | A kind of liquid relief machine |
| CN113702142A (en) * | 2021-09-10 | 2021-11-26 | 杭州米太科技有限公司 | Cell staining instrument |
| CN114689488A (en) * | 2022-04-21 | 2022-07-01 | 伊尔瑞生物科技(江苏)有限公司 | Lymphocyte counting and detecting micro-fluidic device for cell analysis and method thereof |
| CN116084108A (en) * | 2023-02-16 | 2023-05-09 | 杭州萧山桥南织造有限公司 | Infection device for dyeing textile fabric and processing technology thereof |
| CN116959168A (en) * | 2023-07-24 | 2023-10-27 | 滁州市精美家电设备股份有限公司 | Unmanned vending machine with LED show cupboard |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0921799A (en) * | 1995-07-07 | 1997-01-21 | Aloka Co Ltd | Organism tissue treating device and reagent treating unit |
| CN1317370A (en) * | 2000-02-11 | 2001-10-17 | 莱卡显微系统努斯洛赫股份有限公司 | Automatic coloring appts. with heating station |
| CN101021455A (en) * | 2007-03-22 | 2007-08-22 | 广州安必平医药科技有限公司 | Slide-making staining machine and method for slide-making staining method |
| US20110136135A1 (en) * | 2009-12-09 | 2011-06-09 | Larsen Soeren Damgaard | Apparatus and method for processing biological samples |
| CN102607914A (en) * | 2012-03-08 | 2012-07-25 | 张晓东 | Turnover cell tabletting device |
| US20120219469A1 (en) * | 2001-11-26 | 2012-08-30 | Lab Vision Corporation | Automated tissue staining system and reagent container |
| CN102759474A (en) * | 2012-06-13 | 2012-10-31 | 长沙康柏恩医疗科技有限公司 | Liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting |
| WO2013101776A2 (en) * | 2011-12-28 | 2013-07-04 | Abbott Laboratories | Accelerated wright-giemsa and may-grünwald staining methods |
| CN203606196U (en) * | 2013-10-10 | 2014-05-21 | 广州安必平自动化检测设备有限公司 | Cell-staining device |
-
2013
- 2013-10-10 CN CN201310470832.5A patent/CN103558074B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0921799A (en) * | 1995-07-07 | 1997-01-21 | Aloka Co Ltd | Organism tissue treating device and reagent treating unit |
| CN1317370A (en) * | 2000-02-11 | 2001-10-17 | 莱卡显微系统努斯洛赫股份有限公司 | Automatic coloring appts. with heating station |
| US20120219469A1 (en) * | 2001-11-26 | 2012-08-30 | Lab Vision Corporation | Automated tissue staining system and reagent container |
| CN101021455A (en) * | 2007-03-22 | 2007-08-22 | 广州安必平医药科技有限公司 | Slide-making staining machine and method for slide-making staining method |
| US20110136135A1 (en) * | 2009-12-09 | 2011-06-09 | Larsen Soeren Damgaard | Apparatus and method for processing biological samples |
| WO2013101776A2 (en) * | 2011-12-28 | 2013-07-04 | Abbott Laboratories | Accelerated wright-giemsa and may-grünwald staining methods |
| CN102607914A (en) * | 2012-03-08 | 2012-07-25 | 张晓东 | Turnover cell tabletting device |
| CN102759474A (en) * | 2012-06-13 | 2012-10-31 | 长沙康柏恩医疗科技有限公司 | Liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting |
| CN203606196U (en) * | 2013-10-10 | 2014-05-21 | 广州安必平自动化检测设备有限公司 | Cell-staining device |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897980B (en) * | 2014-02-26 | 2016-02-10 | 聂棱 | A kind of liquid transfer gun head inserting device for biomolecules purification & isolation |
| CN103897980A (en) * | 2014-02-26 | 2014-07-02 | 北京中纳海润生物科技有限公司 | Liquid moving gun inserting device for biologic molecule purification and separation |
| CN107760561A (en) * | 2017-10-26 | 2018-03-06 | 深圳市汇研科创生物科技有限公司 | Nucleic acid-extracting apparatus and its application method |
| CN107760561B (en) * | 2017-10-26 | 2019-05-28 | 深圳市汇研科创生物科技有限公司 | Nucleic acid-extracting apparatus and method for extracting nucleic acid |
| CN109297792B (en) * | 2018-10-18 | 2023-12-26 | 广州安必平医药科技股份有限公司 | Slice-making and dyeing integrated machine |
| CN109297794A (en) * | 2018-10-18 | 2019-02-01 | 广州安必平医药科技股份有限公司 | All-in-one machine is dyed in dyed plate and film-making |
| CN109297792A (en) * | 2018-10-18 | 2019-02-01 | 广州安必平医药科技股份有限公司 | All-in-one machine is dyed in film-making |
| CN109297794B (en) * | 2018-10-18 | 2023-12-26 | 广州安必平医药科技股份有限公司 | Dyeing plate and slice-making dyeing integrated machine |
| CN110270389A (en) * | 2019-07-22 | 2019-09-24 | 江苏集萃微纳自动化系统与装备技术研究所有限公司 | A kind of liquid relief machine |
| CN113702142A (en) * | 2021-09-10 | 2021-11-26 | 杭州米太科技有限公司 | Cell staining instrument |
| CN114689488A (en) * | 2022-04-21 | 2022-07-01 | 伊尔瑞生物科技(江苏)有限公司 | Lymphocyte counting and detecting micro-fluidic device for cell analysis and method thereof |
| CN116084108A (en) * | 2023-02-16 | 2023-05-09 | 杭州萧山桥南织造有限公司 | Infection device for dyeing textile fabric and processing technology thereof |
| CN116084108B (en) * | 2023-02-16 | 2023-09-19 | 杭州萧山桥南织造有限公司 | Dip dyeing device for dyeing textile fabric and processing technology thereof |
| CN116959168A (en) * | 2023-07-24 | 2023-10-27 | 滁州市精美家电设备股份有限公司 | Unmanned vending machine with LED show cupboard |
| CN116959168B (en) * | 2023-07-24 | 2024-03-05 | 滁州市精美家电设备股份有限公司 | Unmanned vending machine with LED show cupboard |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103558074B (en) | 2016-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103558074A (en) | Cell-stain apparatus | |
| CN102839738B (en) | Rapidly telescopic spray-washing mechanism | |
| JP2012246143A (en) | Container filling apparatus | |
| US9359138B2 (en) | Assembly/transport apparatus | |
| CN203606196U (en) | Cell-staining device | |
| WO2011059161A1 (en) | Stackable parts feeder | |
| CN112516398A (en) | Portable drug delivery device for obstetrics and gynecology department | |
| CN103659216B (en) | Type steel bar truss floor support plate automatic assembling apparatus | |
| CN104569463B (en) | Rack for test tube rail transport switch and blood examination streamline | |
| CN113398450B (en) | A portable washing ware of dosing for anus intestines branch of academic or vocational study nursing | |
| CN106862517B (en) | Cylinder sleeve blank casting machine mould automatic scrubbing device | |
| CN203266096U (en) | Oil cylinder automatic cleaning and assembly system | |
| KR820002246B1 (en) | Apparatus for removing filter cakes in filter press | |
| CN105772468A (en) | Device for driving spraying pipes in barrel washer to rise and fall | |
| EP1020347B1 (en) | Method and apparatus for conveying works | |
| CN219636176U (en) | Material transferring device for transferring steel | |
| CN101633553B (en) | Hyperboloidal glass tempering upper presser mold device | |
| CN220810862U (en) | Rail mounted bulk grain quantity monitoring facilities | |
| CN110181191A (en) | A kind of automotive seats basin nut-welding equipment | |
| CN211134768U (en) | Cleaning system of hub bearing | |
| CN210916168U (en) | Roller quenching equipment | |
| CN216223096U (en) | High pressure frequency conversion cleaning station is with taking out dirty device | |
| CN204979370U (en) | Packing tube auto -incasing equipment | |
| CN109278763A (en) | For transporting the ferry bus and concrete prefabricated element production line of die station | |
| CN220723959U (en) | Thermal invasion treatment equipment for toughened glass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 510665 Room 301, block B, building 2, Kexin street, Huangpu District, Guangzhou, Guangdong Patentee after: GUANGZHOU LBP AUTOMATED TESTING EQUIPMENT Co.,Ltd. Address before: 510665 room 301-1, building B, No. 10 yayingshi Road, Guangzhou high tech Industrial Development Zone, Guangzhou, Guangdong Patentee before: GUANGZHOU LBP AUTOMATED TESTING EQUIPMENT Co.,Ltd. |