CN103509850A - 检测菌体活细胞的方法及其应用以及引物与试剂盒 - Google Patents
检测菌体活细胞的方法及其应用以及引物与试剂盒 Download PDFInfo
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- CN103509850A CN103509850A CN201210199850.XA CN201210199850A CN103509850A CN 103509850 A CN103509850 A CN 103509850A CN 201210199850 A CN201210199850 A CN 201210199850A CN 103509850 A CN103509850 A CN 103509850A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
- C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
- C12Q2549/125—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using sterilising/blocking agents, e.g. albumin
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
成分 | 含量(g/kg) | 成分 | 含量(g/kg) |
玉米粉 | 230 | 酵母粉 | 20 |
大豆粉 | 210 | 骨粉 | 30 |
麸皮 | 220 | 鱼油 | 10 |
面粉 | 170 | 盐 | 10 |
鱼粉 | 100 | 粗蛋白含量(N×6.25) | 22.37 |
Claims (10)
Priority Applications (1)
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CN201210199850.XA CN103509850B (zh) | 2012-06-14 | 2012-06-14 | 检测菌体活细胞的方法及其应用以及引物与试剂盒 |
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CN201210199850.XA CN103509850B (zh) | 2012-06-14 | 2012-06-14 | 检测菌体活细胞的方法及其应用以及引物与试剂盒 |
Publications (2)
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CN103509850A true CN103509850A (zh) | 2014-01-15 |
CN103509850B CN103509850B (zh) | 2015-06-10 |
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CN201210199850.XA Active CN103509850B (zh) | 2012-06-14 | 2012-06-14 | 检测菌体活细胞的方法及其应用以及引物与试剂盒 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105349669A (zh) * | 2015-11-30 | 2016-02-24 | 武汉轻工大学 | 用于饲料中添加的双歧杆菌的快速定性、定量检测试剂盒及检测方法和应用 |
CN109055514A (zh) * | 2018-04-27 | 2018-12-21 | 曾小敏 | 单增李斯特氏菌活菌的快速检测方法 |
CN113046451A (zh) * | 2021-03-19 | 2021-06-29 | 厦门承葛医学检验实验室有限公司 | 一种菌群的活菌定量方法及其用途 |
CN113265445A (zh) * | 2020-02-14 | 2021-08-17 | 泰斯托生物分析有限公司 | 用于检测活微生物的方法和流体管道系统 |
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2012
- 2012-06-14 CN CN201210199850.XA patent/CN103509850B/zh active Active
Non-Patent Citations (3)
Title |
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J. FUJIMOTO等: "Identification and quantification of viable Bifidobacterium breve strain Yakult in human faeces by using strain-specific primers and propidium monoazide", 《JOURNAL OF APPLIED MICROBIOLOGY》 * |
YANLING HAO等: "Complete Genome Sequence of Bifidobacterium longum subsp. longum BBMN68, a New Strain from a Healthy Chinese Centenarian", 《JOURNAL OF BACTERIOLOGY》 * |
丁圣等: "长双歧杆菌BBMN68对便秘模型小鼠的通便作用", 《食品科学》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105349669A (zh) * | 2015-11-30 | 2016-02-24 | 武汉轻工大学 | 用于饲料中添加的双歧杆菌的快速定性、定量检测试剂盒及检测方法和应用 |
CN105349669B (zh) * | 2015-11-30 | 2019-02-22 | 武汉轻工大学 | 用于饲料中添加的双歧杆菌的快速定性、定量检测试剂盒及检测方法和应用 |
CN109055514A (zh) * | 2018-04-27 | 2018-12-21 | 曾小敏 | 单增李斯特氏菌活菌的快速检测方法 |
CN113265445A (zh) * | 2020-02-14 | 2021-08-17 | 泰斯托生物分析有限公司 | 用于检测活微生物的方法和流体管道系统 |
CN113046451A (zh) * | 2021-03-19 | 2021-06-29 | 厦门承葛医学检验实验室有限公司 | 一种菌群的活菌定量方法及其用途 |
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CN103509850B (zh) | 2015-06-10 |
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