CN103479849B - A kind ofly treat pharmaceutical composition of oyster herpetovirus disease and preparation method thereof - Google Patents
A kind ofly treat pharmaceutical composition of oyster herpetovirus disease and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of pharmaceutical composition for the treatment of oyster herpetovirus disease, comprise following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 15 ~ 30 parts, Herba Erodii 20 ~ 30 parts, Folium Artemisiae Argyi 5 ~ 15 parts, Cortex Phellodendri 10 ~ 25 parts, Flos Chrysanthemi Indici 2 ~ 10 parts, 5 ~ 15 parts, Bulbus Allii.The invention also discloses the preparation method of this pharmaceutical composition.A kind of pharmaceutical composition for the treatment of oyster herpetovirus disease provided by the invention, wherein Radix Polygoni Ciliinerve, Herba Erodii etc. all have very strong antiviral effect, these are as effective ingredient can prevention and therapy oyster herpetovirus be sick well, and treated effect is up to 99%.In preparation method of the present invention, Radix Polygoni Ciliinerve extracts and have employed supercritical CO
2extracting method, make the 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl of Radix Polygoni Ciliinerve effective ingredient and physcion content higher.Simple, the applicable large-scale production of preparation method of the present invention.
Description
Technical field
The present invention relates to the field of Chinese medicines for the treatment of Concha Ostreae disease, be specifically related to a kind ofly treat pharmaceutical composition of oyster herpetovirus disease and preparation method thereof.
Background technology
Oyster herpetovirus particle is hexagon, diameter 70 ~ 90nm, tool monolayer adventitia, the nucleoid that some virion tools are dense, infected Concha Ostreae digestive gland is grey Lycoperdon polymorphum Vitt, sporadic death, this disease often betides the Concha Ostreae of the hot water cultivation of discharging electric station, and morbidity water temperature is 28 ~ 30 DEG C, after water temperature drop, this disease disappears thereupon, and morbidity is closely related with water temperature.
Radix Polygoni Ciliinerve is the tuber of polygonaceae plant floating and thready pulse knotweed.The whole year all can gather, and removes stem and leaf, fibrous root, cleans, cuts into slices and dry.Character identification, tuber is irregular bulk, or slightly cylindrical, long 8-15cm, or longer, diameter 3-7cm, surperficial brown color, or slightly cylindrical, long 8-15cm, or longer, diameter 3-7cm, surperficial brown color.Root head has most stem foot to be pimple shape.Matter is extremely hard, and difficulty fractures, section buff.Root head has most stem foot to be pimple shape.Matter is extremely hard, and difficulty fractures, section buff.In the form of a ring, nearly marrow separately has the light yellow xylem bundle of dispersion to wooden depth yellow.Feeble QI, bitter in the mouth.
Herba Erodii has a broad antifungal spectrum, its flavonoid has inhibitory action to multiple bacillus, to the bacteriostasis of dysentery bacterium F1 strain lower than chlortetracycline, tetracycline, higher than berberine, Herba Andrographis, with chloromycetin and streptomycin close; Its herb decoct also has obvious inhibitory action to staphylococcus aureus, group B streptococcus, streptococcus pneumoniae, micrococcus catarrhalis.This product herb decoct all has obvious inhibitory action to the section 68-1 strain of influenza A virus capital, Asia and the celestial platform strain of parainfluenza virus drum and herpes simplex virus.Herba Erodii tannin and catabolite corilagin thereof have stronger active anticancer, have high inhibition effect to the mutagenic processes of carcinogen benzopyrene-7,8-glycol-9,10-epoxide.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides and a kind ofly treat pharmaceutical composition of oyster herpetovirus disease and preparation method thereof.
Technical scheme: in order to solve the problems of the technologies described above, the invention provides a kind of pharmaceutical composition for the treatment of oyster herpetovirus disease, comprise following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 15 ~ 30 parts, Herba Erodii 20 ~ 30 parts, Folium Artemisiae Argyi 5 ~ 15 parts, Cortex Phellodendri 10 ~ 25 parts, Flos Chrysanthemi Indici 2 ~ 10 parts, 5 ~ 15 parts, Bulbus Allii.
Preferably, its parts by weight are respectively: Radix Polygoni Ciliinerve 20 ~ 30 parts, Herba Erodii 25 ~ 30 parts, Folium Artemisiae Argyi 10 ~ 15 parts, Cortex Phellodendri 15 ~ 25 parts, Flos Chrysanthemi Indici 6 ~ 10 parts, 10 ~ 15 parts, Bulbus Allii.
More preferably, its parts by weight are respectively: Radix Polygoni Ciliinerve 20 parts, Herba Erodii 25 parts, Folium Artemisiae Argyi 10 parts, Cortex Phellodendri 15 parts, Flos Chrysanthemi Indici 6 parts, 10 parts, Bulbus Allii.
The preparation method of described pharmaceutical composition, comprises the following steps:
1) Radix Polygoni Ciliinerve taken by above-mentioned parts by weight adds the ethanol of 80% of its weight 10 ~ 15 times amount, adopts supercritical CO
2extraction obtains Radix Polygoni Ciliinerve effective ingredient;
2) take by above-mentioned parts by weight the ethanol that Herba Erodii adds 75% of its weight 10 ~ 15 times amount and carry out alcohol reflux, extract 3 times, each 2.5h obtains Herba Erodii effective ingredient;
3) by 20 ~ 25 times of soak by water 2 ~ 4h that above-mentioned parts by weight take Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii add Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii gross weight, filter, obtain supernatant;
4) by Radix Polygoni Ciliinerve effective ingredient, the step 2 of step 1)) Herba Erodii effective ingredient and the supernatant mixing of step 3) namely to obtain medical treatment the pharmaceutical composition of oyster herpetovirus disease.
The effective ingredient of described Radix Polygoni Ciliinerve is 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl and physcion.
Described Herba Erodii effective ingredient is p-sitosterol, Quercetin, kaempferol-7-0-rhamnoside, kaempferol and gallic acid.
Described supercritical CO
2extraction conditions is: temperature 50 ~ 60 DEG C, pressure are 27 ~ 30MPa, the quiet hour is 2h, and dynamic time is 1.5h.
Beneficial effect: a kind of pharmaceutical composition for the treatment of oyster herpetovirus disease provided by the invention, wherein Radix Polygoni Ciliinerve, Herba Erodii etc. all have very strong antiviral effect, these are as effective ingredient can prevention and therapy oyster herpetovirus be sick well, and treated effect is up to 99%.In preparation method of the present invention, Radix Polygoni Ciliinerve extracts and have employed supercritical CO
2extracting method, make the 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl of Radix Polygoni Ciliinerve effective ingredient and physcion content higher.Simple, the applicable large-scale production of preparation method of the present invention.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described.
embodiment 1:
Treat a pharmaceutical composition for oyster herpetovirus disease, comprise following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 15 parts, Herba Erodii 20 parts, Folium Artemisiae Argyi 5 parts, Cortex Phellodendri 10 parts, Flos Chrysanthemi Indici 2 parts, 5 parts, Bulbus Allii.
The preparation method of described pharmaceutical composition, comprises the following steps:
1) Radix Polygoni Ciliinerve taken by above-mentioned parts by weight adds the ethanol of 80% of its weight 10 times amount, adopts supercritical CO
2extraction obtains Radix Polygoni Ciliinerve effective ingredient;
2) take by above-mentioned parts by weight the ethanol that Herba Erodii adds 75% of its weight 10 times amount and carry out alcohol reflux, extract 3 times, each 2.5h obtains Herba Erodii effective ingredient;
3) by 20 times of soak by water 2h that above-mentioned parts by weight take Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii add Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii gross weight, filter, obtain supernatant;
4) by Radix Polygoni Ciliinerve effective ingredient, the step 2 of step 1)) Herba Erodii effective ingredient and the supernatant mixing of step 3) namely to obtain medical treatment the pharmaceutical composition of oyster herpetovirus disease.
The effective ingredient of described Radix Polygoni Ciliinerve is 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl and physcion.
Described Herba Erodii effective ingredient is p-sitosterol, Quercetin, kaempferol-7-0-rhamnoside, kaempferol and gallic acid.
Described supercritical CO
2extraction conditions is: temperature 50 C, pressure are 27MPa, the quiet hour is 2h, and dynamic time is 1.5h.
embodiment 2:
Treat a pharmaceutical composition for oyster herpetovirus disease, comprise following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 30 parts, Herba Erodii 30 parts, Folium Artemisiae Argyi 15 parts, Cortex Phellodendri 25 parts, Flos Chrysanthemi Indici 10 parts, 15 parts, Bulbus Allii.
The preparation method of described pharmaceutical composition, comprises the following steps:
1) Radix Polygoni Ciliinerve taken by above-mentioned parts by weight adds the ethanol of 80% of its weight 15 times amount, adopts supercritical CO
2extraction obtains Radix Polygoni Ciliinerve effective ingredient;
2) take by above-mentioned parts by weight the ethanol that Herba Erodii adds 75% of its weight 15 times amount and carry out alcohol reflux, extract 3 times, each 2.5h obtains Herba Erodii effective ingredient;
3) by 25 times of soak by water 4h that above-mentioned parts by weight take Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii add Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii gross weight, filter, obtain supernatant;
4) by Radix Polygoni Ciliinerve effective ingredient, the step 2 of step 1)) Herba Erodii effective ingredient and the supernatant mixing of step 3) namely to obtain medical treatment the pharmaceutical composition of oyster herpetovirus disease.
The effective ingredient of described Radix Polygoni Ciliinerve is 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl and physcion.
Described Herba Erodii effective ingredient is p-sitosterol, Quercetin, kaempferol-7-0-rhamnoside, kaempferol and gallic acid.
Described supercritical CO
2extraction conditions is: temperature 60 C, pressure are 30MPa, the quiet hour is 2h, and dynamic time is 1.5h.
embodiment 3:
Treat a pharmaceutical composition for oyster herpetovirus disease, comprise following Chinese medicine, its parts by weight are respectively: its parts by weight are respectively: Radix Polygoni Ciliinerve 20 parts, Herba Erodii 25 parts, Folium Artemisiae Argyi 10 parts, Cortex Phellodendri 15 parts, Flos Chrysanthemi Indici 6 parts, 10 parts, Bulbus Allii.
The preparation method of described pharmaceutical composition, comprises the following steps:
1) Radix Polygoni Ciliinerve taken by above-mentioned parts by weight adds the ethanol of 80% of its weight 12 times amount, adopts supercritical CO
2extraction obtains Radix Polygoni Ciliinerve effective ingredient;
2) take by above-mentioned parts by weight the ethanol that Herba Erodii adds 75% of its weight 12 times amount and carry out alcohol reflux, extract 3 times, each 2.5h obtains Herba Erodii effective ingredient;
3) by 23 times of soak by water 3h that above-mentioned parts by weight take Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii add Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii gross weight, filter, obtain supernatant;
4) by Radix Polygoni Ciliinerve effective ingredient, the step 2 of step 1)) Herba Erodii effective ingredient and the supernatant mixing of step 3) namely to obtain medical treatment the pharmaceutical composition of oyster herpetovirus disease.
The effective ingredient of described Radix Polygoni Ciliinerve is 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl and physcion.
Described Herba Erodii effective ingredient is p-sitosterol, Quercetin, kaempferol-7-0-rhamnoside, kaempferol and gallic acid.
Described supercritical CO
2extraction conditions is: temperature 56 DEG C, pressure are 28MPa, the quiet hour is 2h, and dynamic time is 1.5h.
Concha Ostreae in the present invention adopts the shed frame type cultivation method oyster culture formed in method that hangs down, and this method mariculture area requires that trend is unimpeded, and flow velocity 20-25 cel, have appropriate fresh water to inject, the physical and chemical factors such as sea area food organisms, water Inversion phenomenon are suitable for Concha Ostreae and form.Culturing area selects the region that can keep the 2-3 rice depth of water when low tidal region or little flood tide, and seawater transparency, more than 0.5 meter, is coated with face smooth, can not be excessively soft, and being coated with matter is sandy or argillo arenaceous.Frame is stub (Phyllostachys pubescens Mazei ex H.de Lebaie corresponding to two row or multirow, Shi Junke) and the polyethylene rope of horizontal laying (or Phyllostachys pubescens Mazei ex H.de Lebaie) composition, the capable following current of stake is arranged, in stake row, two pilespacings are from being 3-4 rice, relative distance between row and row is about 1 meter, stake line length determines the length of stake according to farming site, be generally 3 meters, also determined by beach substrate soft or hard, pile crown major part is vertically squeezed in painting, beach face 0.6-0.8 rice is exposed in upper end, on the top of pile crown, with the polyethylene rope more than 240 (stocks) or Phyllostachys pubescens Mazei ex H.de Lebaie, stake is concatenated by row, polyethylene rope is fixed by the two ends taper pile of stake row, form a fixing low frame.40-45 days after collecting seedling, collects seedling after reaching production requirement, will adopt the collector dispersion of the spat of oyster, the frame moving on to low tidal region is formed.Hung at the two ends of oyster shell collector respectively on the polyethylene rope cultivating frame or Phyllostachys pubescens Mazei ex H.de Lebaie, Seedling string is flat to be hung slightly in parabolic type, between string and string distance 20-25 centimetre, and walk abreast and keep hanging, Seedling string minimum point is from beach face more than 20 centimetres.
Any one of the embodiment of the present invention 1 ~ 3 pharmaceutical composition usage and consumption: the sick burst period of oyster herpetovirus, splash to shed frame type oyster culture region comprehensively, every 1 cubic metre of water body medication 1.3 ~ 2.7g, every mu of water body depth of water 1m pharmaceutical composition 1600 ~ 1800g of the present invention, is used in conjunction 3 days.
Experimental condition:
Category-A oyster culture region: area 1 mu, water depth 1.7 meters, water source is Yangtze River Water, pH is 7.0, and water temperature is 25 DEG C.This region Concha Ostreae 100, average weight are 83g.
Category-B oyster culture region: area 0.9 mu, water depth 1.6 meters, water source is Yangtze River Water, pH is 7.0, and water temperature is 25 DEG C.This pond Concha Ostreae 100, average weight are 82g.
Test method:
Category-A oyster culture region medication 1.8kg, first day is 1 Concha Ostreae in the dust, second day medication 1.8kg, and oyster herpetovirus disease is improved, the 3rd day medication 1.8kg, and oyster herpetovirus disease is cured, and movable and feed recovers normal condition.
Category-B oyster culture region does not have medication, and first day is 16 Concha Ostreaes in the dust, second day 34 in the dust, the 3rd day 50 in the dust.
Result of the test:
Category-A oyster culture region is 1 oyster herpetovirus disease in the dust, and cure rate is 99%, the non-medication in category-B oyster culture region, and mortality rate increases, last all dead.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. treat the pharmaceutical composition of oyster herpetovirus disease for one kind, it is characterized in that, be made up of following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 15 ~ 30 parts, Herba Erodii 20 ~ 30 parts, Folium Artemisiae Argyi 5 ~ 15 parts, Cortex Phellodendri 10 ~ 25 parts, Flos Chrysanthemi Indici 2 ~ 10 parts, 5 ~ 15 parts, Bulbus Allii.
2. the pharmaceutical composition for the treatment of oyster herpetovirus disease according to claim 1, it is characterized in that, be made up of following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 20 ~ 30 parts, Herba Erodii 25 ~ 30 parts, Folium Artemisiae Argyi 10 ~ 15 parts, Cortex Phellodendri 15 ~ 25 parts, Flos Chrysanthemi Indici 6 ~ 10 parts, 10 ~ 15 parts, Bulbus Allii.
3. the pharmaceutical composition for the treatment of oyster herpetovirus disease according to claim 1, it is characterized in that, be made up of following Chinese medicine, its parts by weight are respectively: Radix Polygoni Ciliinerve 20 parts, Herba Erodii 25 parts, Folium Artemisiae Argyi 10 parts, Cortex Phellodendri 15 parts, Flos Chrysanthemi Indici 6 parts, 10 parts, Bulbus Allii.
4. the preparation method of the pharmaceutical composition described in any one of claim 1 ~ 3, is characterized in that, comprises the following steps:
1) Radix Polygoni Ciliinerve taken by above-mentioned parts by weight adds the ethanol of 80% of its weight 10 ~ 15 times amount, adopts supercritical CO
2extraction obtains Radix Polygoni Ciliinerve effective ingredient;
2) take by above-mentioned parts by weight the ethanol that Herba Erodii adds 75% of its weight 10 ~ 15 times amount and carry out alcohol reflux, extract 3 times, each 2.5h obtains Herba Erodii effective ingredient;
3) by 20 ~ 25 times of soak by water 2 ~ 4h that above-mentioned parts by weight take Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii add Folium Artemisiae Argyi, Cortex Phellodendri, Flos Chrysanthemi Indici, Bulbus Allii gross weight, filter, obtain supernatant;
4) by Radix Polygoni Ciliinerve effective ingredient, the step 2 of step 1)) Herba Erodii effective ingredient and the supernatant mixing of step 3) namely to obtain medical treatment the pharmaceutical composition of oyster herpetovirus disease.
5. the preparation method of pharmaceutical composition according to claim 4, is characterized in that, the effective ingredient of described Radix Polygoni Ciliinerve is 9,10-Anthracenedione,1,3,8-thihydroxy-6-methyl and physcion.
6. the preparation method of pharmaceutical composition according to claim 4, is characterized in that, described Herba Erodii effective ingredient is p-sitosterol, Quercetin, kaempferol-7-0-rhamnoside, kaempferol and gallic acid.
7. the preparation method of pharmaceutical composition according to claim 4, is characterized in that, described supercritical CO
2extraction conditions is: temperature 50 ~ 60 DEG C, pressure are 27 ~ 30MPa, the quiet hour is 2h, and dynamic time is 1.5h.
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| JP2535555B2 (en) * | 1987-09-17 | 1996-09-18 | 日清製粉株式会社 | Agent for preventing and treating streptococcal disease in fish |
| CN102727503A (en) * | 2011-04-13 | 2012-10-17 | 东北林业大学 | Application of Geraniin extracted from Geranium sibiricum Linne in preparation of anti-herpes virus medicaments |
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