CN103462212A - Method for improving quality of tobacco leaves by adding enzyme preparation, glucose and citric acid - Google Patents
Method for improving quality of tobacco leaves by adding enzyme preparation, glucose and citric acid Download PDFInfo
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- CN103462212A CN103462212A CN2013104006252A CN201310400625A CN103462212A CN 103462212 A CN103462212 A CN 103462212A CN 2013104006252 A CN2013104006252 A CN 2013104006252A CN 201310400625 A CN201310400625 A CN 201310400625A CN 103462212 A CN103462212 A CN 103462212A
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Abstract
The invention provides a method for improving quality of tobacco leaves by adding enzyme preparation, glucose and citric acid and belongs to the field of tobacco processing. The method includes: spraying, by weight of that of treated tobacco leaves, 0.05%-0.1% of xylanase solution (pH=8.0) onto surfaces of the tobacco leaves prior to placing the tobacco leaves at the temperature ranging from 55 DEG C to 60 DEG C and the humidity of 50-60% in an enclosed environment for 4-10 hours; then spraying, by weight of that of the treated tobacco leaves, 0.1%-0.4% of acid protease solution (pH=3.5) onto the surfaces of the tobacco leaves and subjecting the tobacco leaves to stored-leaves enzymolysis for 3-6 hours at the temperature ranging from 20 DEG C to 30 DEG C and the humidity of 50-60%; adding, by weight of that of the treated tobacco leaves, 2.0%-4.0% of the glucose and 0.5%-1.0% of the citric acid for leaves storage for 5-8 hours in an environment at the temperature ranging from 20 DEG C to 30 DEG C and the humidity of 50-60%, drying the tobacco leaves for 3-10 minutes at the temperature ranging from 110 DEG C to 150 DEG C, and keeping flowing of air and turning of the tobacco leaves to further remove offensive odor and stimulus in the low-grade raw tobacco leaves; placing the well-treated tobacco leaves in an environment of a constant temperature and humidity chamber 22 DEG C in temperature and 60% in humidity for balancing for more than 48 hours till moisture content is equilibrium to 12%, and shredding prior to packing the tobacco leaves. By the method, tobacco smoke strength, the stimulus and the offensive odor can be lowered, pureness and mildness can be enhanced, and aroma can be harmonized.
Description
Technical field
The present invention relates to a kind of method that improves quality of tobacco by adding enzyme preparation and glucose and combined citric acid high-temperature process, both belonged to tobacco leaf processing field, also belong to the biological enzyme formulation field.
Technical background
Reduced sugar in tobacco in carbohydrate is to affect one of jealous main chemical compositions of tobacco, and sugared content is high within the specific limits, and quality of tobacco is good, but sugar content is too high or too low, all unfavorable to quality of tobacco; The thermal decomposition product of nitrogen-containing compound (meaning with nitrogen pool) when cigarette burns and sucks generally is alkalescence, and strong impulse and pungent burnt bitter taste are arranged more; Appropriate nicotine can meet smoker's physiological requirements, but too high levels, and flue gas strength is large, increases excitant.Therefore, they have important effect to the quality of improving tobacco product.By the suction of the sense organ to tangerine two (B2F) raw tobacco material on Shaoyang, Hunan, find mainly to have the following disadvantages: excitant is large, and strength is larger, and flue gas concentration is larger, and fragrance matter is medium, and assorted gas is large, and pleasant impression is poor.Just because of above deficiency, cause it to be difficult to be applied to tobacco leaf formulation.Further the Essential Chemistry constituent analysis of tangerine on Shaoyang two (B2F) raw tobacco material is found, the content of Water-soluble Total Sugar and reduced sugar is on the low side, and content is respectively 13.10% and 12.31%; Protein content is higher, and content is 7.12%; Total nicotine content higher 3.96%; Sugar alkali ratio is 3.31, obviously on the low side.Therefore, under the guidance of basic chemical composition analysis result, for Shaoyang B2F raw tobacco material excitant and the large characteristics of strength, carry out the upgrading processing, this is significant for solving its application limitation and upgrading increment.
In tobacco industry, the method that reduces tobacco leaf suction excitant and strength has flavoring and casing method, method of chemical treatment and biotransformation method.Modal in biotransformation method is to use the cellulase treatment tobacco leaf, and the cellulase treatment tobacco leaf is the important method that improves cigarette quality, and it has technique simply, does not need the advantages such as specific condition, pollution-free material discharge-ring border such as HTHP.The cellulase treatment tobacco leaf is all to utilize complex enzyme to carry out both at home and abroad at present.Because different tobacco leaves has different inherent constituent contents and different tobacco leaves to suck style, many tobacco leaves have changed original style after complex enzyme is processed, and have even increased exogenous impurity.This compatibility for different tobacco leaves and maintenance cigarette Brand characteristic are very disadvantageous, give compatibility and keep the cigarette Brand characteristic to bring difficulty.Therefore screening the method that can improve quality of tobacco and the enzyme preparation of shortening alcoholization phase and process tobacco leaf under the prerequisite that keeps original tabacco fragrance style is of great significance.In addition, Shaoyang, Hunan B2F raw tobacco material through the independent undertreatment of enzyme so that enough flavor precursors to be provided, reduce excitant and the assorted gas of flue gas, therefore need add reduced sugar and citric acid according to the inharmonic characteristics of sugared alkali ratio of raw tobacco material itself, by raising, burn and suck the occurrence degree of Maillard reaction in process and reach the purpose of upgrading in conjunction with free nicotine.Simultaneously, further high-temperature heat treatment higher fatty acids and the nicotine in tobacco leaf of can also degrading, reduce flue gas excitant and assorted gas, increases alcohol and property, coordinates fragrance.
In Chinese patent application 87101362, that the complex enzyme of koning trichoderma fermentation is processed to discarded tobacco leaf, can improve a tobacco leaf 0.5-1 grade, but this invention is that tobacco leaf is placed at 60%-70% relative humidity, 30 ℃ of-50 ℃ of temperature, enzymolysis 22-26 hour, the method time is partially long, and the original aromatic style of tobacco leaf can not get ensureing, and easy breed bacteria.In Chinese patent application 03114578.7, a kind of complex enzyme and technology of preparing thereof for improving quality of tobacco disclosed, its composition is comprised of cellulase, protease, invertase, maltose, lysozyme, can be glucose and amino acid by the cellulose in tobacco leaf, protein degradation, and transformed, increase tabacco fragrance, but this invention complex enzyme making is loaded down with trivial details, and independent enzymatic treatment solution changes original tabacco fragrance style, can't obtain satisfied effect.In Chinese patent application 200810204482.7, adopt the method for improving quality of tobacco leaf with proteinase biotransformation, there is equally the problem of above-mentioned several patents.The present invention has well solved some limitation of above-mentioned related invention patent, has both obtained satisfied effect, also is suitable for large-scale industrial application.
Summary of the invention
The weak point existed for present technology, the invention provides a kind of method that fully utilizes enzyme preparation and add Maillard reaction precursor associating high-temperature heat treatment, can keep under the prerequisite of original tabacco fragrance style, be mainly used in reducing tobacco leaf excitant and strength, thereby improve tobacco leaf aesthetic quality's method.
Purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, percentage of the present invention is percetage by weight.
A kind of method that improves quality of tobacco by adding enzyme preparation and glucose and citric acid, is characterized in that, comprises following steps:
(1) once reinforced: as enzyme preparation zytase solution to be put on to tobacco leaf surface in the mode of spraying, stir tobacco leaf during sprinkling, make to spray evenly, keep tobacco leaf loose; After sprinkling, tobacco leaf is placed in enclosed environment and processes, reaction temperature 50-60 ℃, ambient humidity 50-60%, reaction time 4-10h.
(2) secondary charging: enzyme preparation acidic protein enzyme solutions is put on to tobacco leaf surface in the mode of spraying, stir tobacco leaf during sprinkling, make to spray evenly, keep tobacco leaf loose; After sprinkling, tobacco leaf is placed in enclosed environment and processes, reaction temperature 25-35 ℃, ambient humidity 50-60%, reaction time 3-6h.
(3) three times reinforced: after enzymolysis finishes, add glucose and citric acid compound material, in the mode of spraying, put on tobacco leaf surface, stir tobacco leaf during sprinkling, make to spray evenly, keep tobacco leaf loose; After sprinkling, tobacco leaf is placed in enclosed environment and processes, reaction temperature 20-30 ℃, ambient humidity 50-60%, reaction time 5-8h.
(4) after leaves storage completes, carry out drying and process under the high-temperature heat treatment condition, obtain more high-quality tobacco leaf.
The preferred zytase consumption of the present invention is for processing the 0.05-0.1% of tobacco leaf weight.For enzyme reaction is more easily carried out, the preferred zytase pH value of solution of the present invention is adjusted to 8.0; The reagent of regulating the zytase pH value of solution can be potassium acetate or potassium citrate.
Preferred acid protease consumption of the present invention is for processing the 0.1-0.4% of tobacco leaf weight.For the secondary enzyme reaction is more easily carried out, preferred acid protease pH value of solution of the present invention is adjusted to 3.5; The reagent of adjustment of acidity protein enzyme solution pH can be citric acid or acetic acid.
Compound material of the present invention, the glucose consumption is preferably the 2.0-4.0% that processes tobacco leaf weight, and Citric Acid Dosage is preferably the 0.5-1.0% that processes tobacco leaf weight.After reinforced the processing, tobacco leaf is placed under 110-150 ℃ of temperature conditions and carries out high-temperature heat treatment oven dry 3-10min; Keep flowing of air and stirring of tobacco leaf, further remove assorted gas and stimulation in the inferior grade raw tobacco.
The raw tobacco material that the present invention uses is selected from the tobacco leaf in other places of production of tangerine two (B2F) on Shaoyang, Hunan or ad eundem.
If in advance tobacco leaf is first carried out to removal of impurities simply and pretreatment: smoked sheet is scattered, reject offal, foreign material etc.; Spray the deionized water leaves moisting, making moisture (w/w) is 18%～20%, will more be conducive to improve quality of tobacco.
Above method is processed to the more high-quality tobacco leaf obtained and be cut into silk, more than being placed in constant-temperature constant-humidity environment balance 48h, moisture content of cut tobaccos is controlled as 11.5%-12.5%, is required cigarette shreds.22 ℃ of the preferred temperature of constant-temperature constant-humidity environment, humidity 60%.
The usefulness of technique scheme is:
The inventive method adopts Multistep feeding to process tobacco leaf, at first add zytase and generate water-soluble sugar for the tobacco leaf cellulose of degrading, destroy the tobacco cell cell membrane simultaneously, make intracellular organic matter expose as protein, more easily by the degraded of protease enzyme, generated amino acid and peptide, and Maillard reaction at high temperature occurs with water-soluble sugar (comprising the glucose of adding), produce fragrance matter; In born of the same parents, taste compound also presents as volatile oil, organic acid etc. simultaneously, increases tobacco leaf usability; Then add acid protease, the protein in the degraded tobacco leaf generates free amino acid and peptide class, when reducing protein content, improves and burns and sucks Maillard reaction precursor substance in process; The glucose added and citric acid and enzymolysis product peptide and the acting in conjunction of amino acid whose high-temperature split product small molecular acidity material are in conjunction with free nicotine, can suitably reduce excitant and the strength of cigarette smoke, high-temperature heat treatment can also degrade higher fatty acids and nicotine in tobacco leaf, reduce flue gas excitant and assorted gas, increase alcohol and property, coordinate fragrance.The method not only can be processed other place of production tobacco leaves of ad eundem, the reconstituted tobacoo that is applicable to too pipe tobacco, stem and reproduces.
The specific embodiment
Further set forth content of the present invention below in conjunction with specific embodiment, but embodiment is not limitation of the invention.
Embodiment 1
Select tangerine two (B2F) raw tobacco material on Shaoyang, Hunan, smoked sheet is scattered, reject offal, foreign material etc.; Spray the deionized water leaves moisting, making moisture (w/w) is 18%～20%, once feed in raw material, spray zytase liquid by 0.05% the amount of processing tobacco leaf weight and (use deionized water dissolving, regulate pH=8.0 with potassium acetate) simultaneously, after spraying end, at temperature 60 C, after carrying out leaves storage 4h under the condition of humidity 60%; Carry out secondary charging, by 0.1% the amount of processing tobacco leaf weight, spray acid protease (use deionized water dissolving, regulate pH=3.5 with citric acid simultaneously), after spraying end, 35 ℃ of temperature, carry out leaves storage 6h under the condition of humidity 60%; Then adding compound material (forms by the amount glucose of processing tobacco leaf weight 2.0% and 0.5% citric acid, by deionized water dissolving.), 20 ℃ of temperature, leaves storage 5h under the condition of humidity 60% turns over the above tobacco leaf of handling well baking 3min processed in 150 ℃ of baking ovens.Then adopt tobacco Special experimental chamber Miniature filament cutter to carry out chopping, tobacco cut width 1mm.Finally be placed on 22 ℃ of temperature, in the climatic chamber of humidity 60% more than balance 48h, until moisture is while being equilibrated at 11.5%, cigarette, carry out chemical composition analysis and sensory evaluation.
As can be seen from Table 1, after processing, the protein content of pipe tobacco has reduced by 10.96%, and reproducibility total reducing sugar increasing degree is 32.52%, and total nicotine reduces by 7.99%, and sugared alkali is than increasing by 44.15%, and this is very favorable to the grade quality that improves tobacco leaf.
As can be seen from Table 2, each alkaloid after processing in flue gas lowers, nicotine reduces 32.64%, Mai Siming reduces 51.50%, anabasine reduces 43.80%, 2,3-bipyridyl and reduces 40.57%, cotinine reduces 31.31%, and the minimizing of these alkaloid components has important function to strength and the excitant of improving tobacco leaf.
From table 3 subjective appreciation, can find out, the sample after processing is compared with control sample, and flue gas is finer and smoother, soft, on assorted gas, strength, excitement, obviously reduces, and pleasant impression is clean, slightly pleasantly sweet, and quality of tobacco has obtained promoting preferably.
Table 1 is processed front and back each related substances content of pipe tobacco
Table 2 is processed front and back flue gas alkaloids (%) and is changed
Table 3 is processed the front and back organoleptic feature relatively
Embodiment 2
Select tangerine two (B2F) raw tobacco material on Shaoyang, Hunan, smoked sheet is scattered, reject offal, foreign material etc.; Spray the deionized water leaves moisting, making moisture (w/w) is 18%～20%, once feed in raw material, spray zytase liquid by 0.1% the amount of processing tobacco leaf weight and (use deionized water dissolving, regulate pH=8.0 with potassium citrate) simultaneously, after spraying end, 55 ℃ of temperature, after carrying out leaves storage 10h under the condition of humidity 50%; Carry out secondary charging, by 0.4% the amount of processing tobacco leaf weight, spray acid protease (use deionized water dissolving, regulate pH=3.5 with acetic acid simultaneously), after spraying end, 25 ℃ of temperature, carry out leaves storage 3h under the condition of humidity 50%; Then adding compound material (forms by the amount glucose of processing tobacco leaf weight 4.0% and 1.0% citric acid, by deionized water dissolving), 30 ℃ of temperature, leaves storage 8h under the condition of humidity 50% turns over the above tobacco leaf of handling well baking 10min processed in 110 ℃ of baking ovens.Then adopt tobacco Special experimental chamber Miniature filament cutter to carry out chopping, tobacco cut width 1mm.Finally be placed on 22 ℃ of temperature, in the climatic chamber of humidity 60% more than balance 48h, until moisture is while being equilibrated at 12.5%, cigarette, carry out chemical composition analysis and sensory evaluation.
As can be seen from Table 4, after processing, the protein content of pipe tobacco has reduced by 14.33%, and reproducibility total reducing sugar increasing degree is 24.88%, and total nicotine has reduced by 8.38%, and sugared alkali ratio has increased by 36.79%, and this is very favorable to the grade quality that improves tobacco leaf.
As can be seen from Table 5, each alkaloid after processing in flue gas lowers, nicotine reduces 29.59%, Mai Siming reduces 48.85%, anabasine reduces 41.68%, 2,3-bipyridyl and reduces 35.14%, cotinine reduces 14.57%, and the minimizing of these alkaloid components has important function to strength and the excitant of improving tobacco leaf.
From table 6 subjective appreciation, can find out, the sample after processing is compared with control sample, and flue gas is finer and smoother, soft, on assorted gas, strength, excitement, obviously reduces, and pleasant impression is clean, slightly pleasantly sweet, and quality of tobacco has obtained promoting preferably.
Table 4 is processed front and back each related substances content of pipe tobacco
Table 5 is processed front and back flue gas alkaloids (%) and is changed
Table 6 is processed the front and back organoleptic feature relatively
Embodiment 3
Select tangerine two (B2F) raw tobacco material on Shaoyang, Hunan, smoked sheet is scattered, reject offal, foreign material etc.; Spray the deionized water leaves moisting, making moisture (w/w) is 18%～20%, once feed in raw material, spray zytase liquid by 0.08% the amount of processing tobacco leaf weight and (use deionized water dissolving, regulate pH=8.0 with potassium citrate) simultaneously, after spraying end, at temperature 50 C, after carrying out leaves storage 8h under the condition of humidity 55%; Carry out secondary charging, by 0.3% the amount of processing tobacco leaf weight, spray acid protease (use deionized water dissolving, regulate pH=3.5 with acetic acid simultaneously), after spraying end, 30 ℃ of temperature, carry out leaves storage 5h under the condition of humidity 55%; Then add compound material (forming by the amount glucose of processing tobacco leaf weight 3.0% and 0.8% citric acid, by deionized water dissolving), 25 ℃ of temperature, leaves storage 6h under the condition of humidity 55% turns over the above tobacco leaf of handling well baking 7min processed in 125 ℃ of baking ovens.Then adopt tobacco Special experimental chamber Miniature filament cutter to carry out chopping, tobacco cut width 1mm.Finally be placed on 22 ℃ of temperature, in the climatic chamber of humidity 60% more than balance 48h, until moisture is while being equilibrated at 12.0%, cigarette, carry out chemical composition analysis and sensory evaluation.
As can be seen from Table 7, after processing, the protein content of pipe tobacco has reduced by 27.11%, and reproducibility total reducing sugar increasing degree is 46.26%, and total nicotine has reduced by 14.38%, and sugared alkali ratio has increased by 70.90%, and this is very favorable to the grade quality that improves tobacco leaf.
As can be seen from Table 8, each alkaloid after processing in flue gas lowers, nicotine reduces 35.96%, Mai Siming reduces 51.28%, anabasine reduces 48.33%, 2,3-bipyridyl and reduces 38.36%, cotinine reduces 36.25%, and the minimizing of these alkaloid components has important function to strength and the excitant of improving tobacco leaf.
From table 9 subjective appreciation, can find out, the sample after processing is compared with control sample, and flue gas is finer and smoother, soft, on assorted gas, strength, excitement, obviously reduces, and pleasant impression is clean, slightly pleasantly sweet, and quality of tobacco has obtained promoting preferably.
Table 7 is processed front and back each related substances content of pipe tobacco
Table 8 is processed front and back flue gas alkaloids (%) and is changed
Table 9 is processed the front and back organoleptic feature relatively
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within principle of the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (11)
1. one kind is passed through to add the method that enzyme preparation and glucose and citric acid improve quality of tobacco, it is characterized in that, comprise following steps: (1) is once reinforced: enzyme preparation zytase solution is put on to tobacco leaf surface in the mode of spraying, stir tobacco leaf during sprinkling, make to spray evenly, keep tobacco leaf loose; After sprinkling, tobacco leaf is placed in enclosed environment and processes, reaction temperature 50-60 ℃, ambient humidity 50-60%, reaction time 4-10h.
(2) secondary charging: enzyme preparation acidic protein enzyme solutions is put on to tobacco leaf surface in the mode of spraying, stir tobacco leaf during sprinkling, make to spray evenly, keep tobacco leaf loose; After sprinkling, tobacco leaf is placed in enclosed environment and processes, reaction temperature 25-35 ℃, ambient humidity 50-60%, reaction time 3-6h.
(3) three times reinforced: after enzymolysis finishes, add glucose and citric acid compound material, in the mode of spraying, put on tobacco leaf surface, stir tobacco leaf during sprinkling, make to spray evenly, keep tobacco leaf loose; After sprinkling, tobacco leaf is placed in enclosed environment and processes, reaction temperature 20-30 ℃, ambient humidity 50-60%, reaction time 5-8h.
(4) after leaves storage completes, carry out drying and process under the high-temperature heat treatment condition, obtain more high-quality tobacco leaf.
2. improve according to claim 1 the method for quality of tobacco, it is characterized in that, described zytase consumption is for processing the 0.05-0.1% of tobacco leaf weight.
3. improve according to claim 2 the method for quality of tobacco, it is characterized in that, described zytase pH value of solution is adjusted to 8.0, and the reagent of regulating pH is potassium acetate or potassium citrate.
4. improve according to claim 3 the method for quality of tobacco, it is characterized in that, described acidic protein enzyme dosage is for processing the 0.1-0.4% of tobacco leaf weight.
5. improve according to claim 4 the method for quality of tobacco, it is characterized in that, described acid protease pH value of solution is adjusted to 3.5, and the reagent of regulator solution pH is citric acid or acetic acid.
6. improve according to claim 5 the method for quality of tobacco, it is characterized in that, the glucose consumption is for processing the 2.0-4.0% of tobacco leaf weight, and Citric Acid Dosage is for processing the 0.5-1.0% of tobacco leaf weight.
7. method according to claim 6, is characterized in that, described high-temperature heat treatment is that tobacco leaf is dried to 3-10min under 110-150 ℃ of temperature conditions.
8. improve according to claim 7 the method for quality of tobacco, it is characterized in that, the tobacco leaf that described raw tobacco material is other places of production of tangerine two (B2F) or ad eundem on Shaoyang, Hunan.
9. according to the method for the arbitrary described raising quality of tobacco of claim 1-8, it is characterized in that, also comprise the tobacco leaf pre-treatment step: smoked sheet is scattered, reject offal, foreign material etc.; Spray the deionized water leaves moisting, making moisture (w/w) is 18%～20%.
10. a method that improves quality of tobacco shreds, is characterized in that, the tobacco leaf that the described method of claim 9 is obtained is cut into silk, and more than being placed in constant-temperature constant-humidity environment balance 48h, moisture content of cut tobaccos is controlled as 11.5%-12.5%, is required cigarette shreds.
11. improve according to claim 10 the method for quality of tobacco shreds, it is characterized in that, described constant-temperature constant-humidity environment refers to 22 ℃ of temperature, humidity 60%.
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| CN104223348A (en) * | 2014-09-25 | 2014-12-24 | 云南中烟工业有限责任公司 | Method by combining biological enzyme and microwave expansion technologies to improve quality of tobacco stems |
| CN104489914A (en) * | 2014-11-20 | 2015-04-08 | 湖南中烟工业有限责任公司 | Annexing agent and method for improving upper flue-cured tobacco leaf quality in tobacco leaf production link |
| CN105266188A (en) * | 2015-11-25 | 2016-01-27 | 红云红河烟草(集团)有限责任公司 | Method utilizing compound enzyme preparation to improve penetration capability of feed liquid into tobacco slices |
| CN105661626A (en) * | 2016-03-25 | 2016-06-15 | 河南中烟工业有限责任公司 | Composite biological agent microcapsule capable of improving sensory quality of tobacco |
| CN105831800A (en) * | 2016-05-27 | 2016-08-10 | 山东中烟工业有限责任公司 | Compound enzyme preparation for improving tobacco quality and application thereof |
| CN111528513A (en) * | 2020-04-30 | 2020-08-14 | 景延秋 | Method for strengthening tobacco style |
| CN114766713A (en) * | 2022-04-30 | 2022-07-22 | 中国烟草总公司郑州烟草研究院 | Cigarette cut tobacco making process based on improvement of permeability of surface layer of tobacco lamina |
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| CN104223348B (en) * | 2014-09-25 | 2016-02-24 | 云南中烟工业有限责任公司 | A kind of method improving cabo quality in conjunction with cellulase treatment and microwave expansion technology |
| CN104489914A (en) * | 2014-11-20 | 2015-04-08 | 湖南中烟工业有限责任公司 | Annexing agent and method for improving upper flue-cured tobacco leaf quality in tobacco leaf production link |
| CN105266188A (en) * | 2015-11-25 | 2016-01-27 | 红云红河烟草(集团)有限责任公司 | Method utilizing compound enzyme preparation to improve penetration capability of feed liquid into tobacco slices |
| CN105661626A (en) * | 2016-03-25 | 2016-06-15 | 河南中烟工业有限责任公司 | Composite biological agent microcapsule capable of improving sensory quality of tobacco |
| CN105831800A (en) * | 2016-05-27 | 2016-08-10 | 山东中烟工业有限责任公司 | Compound enzyme preparation for improving tobacco quality and application thereof |
| CN105831800B (en) * | 2016-05-27 | 2017-09-01 | 山东中烟工业有限责任公司 | A kind of complex enzyme formulation for improving quality of tobacco and its application |
| CN111528513A (en) * | 2020-04-30 | 2020-08-14 | 景延秋 | Method for strengthening tobacco style |
| CN114766713A (en) * | 2022-04-30 | 2022-07-22 | 中国烟草总公司郑州烟草研究院 | Cigarette cut tobacco making process based on improvement of permeability of surface layer of tobacco lamina |
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