CN105661626A - Composite biological agent microcapsule capable of improving sensory quality of tobacco - Google Patents
Composite biological agent microcapsule capable of improving sensory quality of tobacco Download PDFInfo
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- CN105661626A CN105661626A CN201610174838.1A CN201610174838A CN105661626A CN 105661626 A CN105661626 A CN 105661626A CN 201610174838 A CN201610174838 A CN 201610174838A CN 105661626 A CN105661626 A CN 105661626A
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
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Abstract
The invention belongs to the technical field of tobacco processing, in particular relates to a composite biological agent microcapsule capable of improving the sensory quality of tobacco. The composite biological agent microcapsule is prepared from a core material and a wall material which are mixed uniformly through spray drying, wherein the core material is a mixture of bacillus subtilis bacterial liquid, sphingol monad bacterial liquid and alkaline protease; the wall material is a mixed solution which is prepared by mixing corn syrup, Arabic gum and maltodextrin and has a 30 to 50 percent of mass concentration. According to the composite biological agent microcapsule disclosed by the invention, a microbial preparation and an enzymic preparation are coated through a microencapsulation technology, and a micro environment which is beneficial for survival of microorganisms is formed, so that the microbial preparation and biological enzyme can favorably take effect on the surface of the tobacco within a longer time, degradation of macromolecular substances in the tobacco is quickened, and the mellowing period of the tobacco is shortened; the effect of improving the quality of the tobacco is relatively obvious, the mellowing time of the tobacco can be obviously shortened, and for a tobacco production enterprise, the composite biological agent microcapsule has relative important application value.
Description
Technical field
The invention belongs to technical field of tobacco processing, be specifically related to a kind of composite biological agent microcapsule for improving tabacum sensory.
Background technology
Tobacco mellowing is the crucial processing link improving tabacum sensory, and Nicotiana tabacum L. is through gathering, after beating and double roasting, and generally also needing to alcoholization cycle of 2 to three years can be only achieved the application quality of the best. By refining the industrial applicability improved Nicotiana tabacum L. interior quality, improve Nicotiana tabacum L., it is always up one of focus of Nicotiana tabacum L. research work.
The essence of tobacco mellowing process is the chemical degradation conversion of various complexity, biological enzyme converts and microorganism promotes the synergistic processes such as conversion, its cardinal principle is to form little molecule by macromolecular substances (such as protein, starch, polyphenol, the pigment etc.) degraded in Nicotiana tabacum L. cause aroma compounds or cause fragrant precursor substance, thus improving the quality of Nicotiana tabacum L.. There are some researches show, utilize suitable microorganism and enzyme preparation can effectively accelerate tobacco mellowing process, and then improve quality of tobacco. Thus the complex microorganism preparations of the exploitation multiple macromolecular substances of degradable, it is the hot research direction improving most application prospect in the research of tobacco mellowing process.
But existing microorganism and enzyme preparation there is also certain restriction and a difficult problem when being applied to tobacco mellowing, one is that the screening of suitable microbial strains still comparatively lacks, two is that microorganism and enzyme effect need certain condition, comparatively strict especially for Environmental Water demand, and during tobacco mellowing, in order to avoid high moisture content to be caused, mildew phenomena occurred, generally require the moisture in strict restriction Nicotiana tabacum L., and too low moisture obviously again limit microorganism and the performance of enzyme preparation activity. Therefore the application in tobacco mellowing process of microorganism and enzyme preparation is limited largely.
Microcapsule technology is a kind of technology being wrapped in by trace substance in the wall materials such as thin polymer film, is the microdisplay package technology of a kind of store solids, liquid, gas. Refer to specifically and a certain goal object (core or interior phase) is coated with by various natural or synthesis macromolecular compound or inorganic material (wall or foreign minister); protection core is played in the shielding action relying on cyst wall, then passes through some outside stimulus (temperature, pressure etc.) or slow releasing function makes the function of goal object again present in outside. This technology is in field extensive uses such as food, cosmetics, feedstuff, essence and flavoring agents at present.
In prior art, kept the existing greater advance of research of microbial activity by microencapsulation technology, but have not yet to see the report that preferably this technology is used for tobacco mellowing.
Summary of the invention
It is an object of the present invention to provide a kind of composite biological agent microcapsule for improving tabacum sensory, after using this composite biological agent microcapsule, it is possible not only to improve tabacum sensory, and the tobacco mellowing cycle can be shortened, thus offering reference and reference for the application in tobacco mellowing production process of new microorganism formulation, also production efficiency for improving tobacco industry establishes good basis simultaneously.
The detailed technology scheme that the present invention takes is described below.
A kind of composite biological agent microcapsule for improving tabacum sensory, this composite biological agent microcapsule is prepared from by spray drying by core and the wall material of mix homogeneously;
Described core is the mixture of bacillus subtilis bacterium solution, Sphingol single-cell bacterium solution and alkaline protease, wherein in bacillus subtilis bacterium bacterium solution, thalline quantity is 40 ~ 6,000,000,000/mL, in Sphingol single-cell bacterium solution, thalline quantity is 10 ~ 3,000,000,000/mL, and described alkaline protease enzyme is lived as 100-400U/mg; By quality ratio, bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: enzyme=0.8 ~ 1.0:0.8 ~ 1.0:1.0 ~ 1.2,
Described wall material is the mixed liquor that mass concentration is 30 ~ 50% that corn syrup, arabic gum and maltodextrin mix, by quality ratio, in mixed liquor, and corn syrup: arabic gum: maltodextrin=3:5:4.
The described composite biological agent microcapsule for improving tabacum sensory, in core by quality ratio, bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: enzyme=1.0:1.0:1.2.
The described composite biological agent microcapsule for improving tabacum sensory, by quality ratio, in composite biological agent microcapsule, core: wall material=1:1.
The preparation method of the described composite biological agent microcapsule for improving tabacum sensory, specifically includes following steps:
(1) core is prepared:
Bacillus subtilis bacterium solution is prepared as follows forming: takes bacillus subtilis strain, LB fluid medium, 30 DEG C, the cultivation amplification of 200rpm shaking table, with aseptic culture fluid for blank, records the bacterium solution absorbance OD at 600nm place600, when in bacterium solution, thalline quantity reaches 40 ~ 6,000,000,000/mL, OD600≈ 6.0, now as application microbial inoculum; Described bacillus subtilis, is specifically: the bacillus subtilis strain xp of CCTCCNo:M2011452 that its Classification And Nomenclature is Bacillussubtilis for example with the preserving number of China typical culture collection center;
Sphingol single-cell bacterium solution is prepared as follows forming: takes Sphingomonas, inorganic salt liquid culture medium, 30 DEG C, the cultivation amplification of 200rpm shaking table, with aseptic culture fluid for blank, records the absorbance OD at 600nm place of bacterium solution600, when in bacterium solution, thalline quantity reaches 10 ~ 3,000,000,000/mL, OD600≈ 2.0, now as application microbial inoculum; Described Sphingol single-cell, the preserving number specifically adopting such as China typical culture collection center is: the Sphingomonas xp of CCTCCNo:M2011453, and its Classification And Nomenclature is Sphingomonassp.;
Prepare described inorganic salt liquid culture medium during Sphingol single-cell bacterium solution, concrete every liter of culture medium contains: chlorogenic acid 0.2g; Na2HPO4·12H2O6.15g; KH2PO41.52g; (NH4)2SO41.5g; MgSO4·7H2O0.20g; CaCl2·2H2O0.05g;
It is 100-400U/mg that the enzyme of described alkaline protease is lived, and specifically can adopt a kind of enzyme alkaline protease commercially produced product for 200U/mg alive of such as Amresco company;
Bacillus subtilis bacterium solution, Sphingol single-cell bacterium solution, alkaline protease are prepared respectively, mix homogeneously is compared by following quality again before preparing composite biological agent microcapsule, bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: enzyme=0.8 ~ 1.0:0.8 ~ 1.0:1.0 ~ 1.2, optimum ratio is, bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: enzyme=1.0:1.0:1.2;
(2) wall material is prepared:
Corn syrup, arabic gum and maltodextrin are mixed in proportion, prepares into solution that mass concentration is 30 ~ 50% as wall material solution;
By quality ratio, corn syrup: arabic gum: maltodextrin=3:5:4;
(3) composite biological agent microcapsule is prepared:
Take wall material solution in step (2), under stirring condition, be proportionally added in step (1) core of mix homogeneously, as material solution after mix homogeneously; By quality ratio, wall material: core=1:1;
By this material solution 40 ~ 60 DEG C, 30 ~ 40MPa when high pressure homogenizing, form stable feed liquid;
The stable feed liquid formed is carried out spray drying in spray drying tower, and design parameter is: spraying feeding temperature is 50 DEG C ~ 60 DEG C, and feed rate is 130 ~ 150mL/min, and inlet temperature is 170 DEG C ~ 180 DEG C, and leaving air temp is 50 DEG C ~ 60 DEG C;
Final gained powder-product is the composite biological agent microcapsule in the application.
The application in tobacco mellowing of the described composite biological agent microcapsule for improving tabacum sensory, composite biological agent microcapsule is mixed by less than the 2.5% of quality of tobacco, under optimum condition, mix by the 1.0 ~ 2.5% of quality of tobacco, further, mix by the 2% of quality of tobacco; After tobacco warehouse deposits 4 ~ 8 months under natural conditions, can obviously improve tabacum sensory.
In the present invention, by microencapsulation technology, microorganism formulation and enzyme preparation are wrapped up, define the microenvironment being beneficial to microbial survival, play a role within the tobacco leaf surface long period thus being beneficial to microorganism formulation and enzyme, thus accelerating the degraded of macromolecular substances in Nicotiana tabacum L., shorten the tobacco mellowing cycle. Actually detected showing, the microorganism in composite biological agent microcapsule provided by the present invention can keep good biological activity in longer time period, and does not rely on the microenvironment in Nicotiana tabacum L.; And it is comparatively obvious to improve effect for quality of tobacco, it is possible to substantially shorten the tobacco mellowing time, for tobacco leaf production enterprise, there is comparatively important using value.
Accompanying drawing explanation
Fig. 1 is the structural representation of the composite biological agent microcapsule for improving tabacum sensory provided by the present invention, and wherein A is the core within composite biological agent microcapsule, and B is the wall material of composite biological agent microcapsule outer wrap.
Detailed description of the invention
Below in conjunction with embodiment the present invention will be further explained explanation. Before introducing specific embodiment, partial material situation involved in the present invention is briefly introduced explanation as follows.
Main raw material(s):
The preserving number of bacillus subtilis employing China typical culture collection center is: the bacillus subtilis strain xp of CCTCCNo:M2011452, and its Classification And Nomenclature is Bacillussubtilis; In the present embodiment, adopted bacterial strain is so kind as to give offer by former depositary institution; Certainly, this bacterial strain also can obtain from above-mentioned preservation mechanism; For guaranteeing technique effect, it is possible to adopt the bacterial strain that other are of the same race;
The preserving number of Sphingol single-cell employing China typical culture collection center is: the Sphingomonas xp of CCTCCNo:M2011453, and its Classification And Nomenclature is Sphingomonassp.; In the present embodiment, adopted bacterial strain is so kind as to give offer by former depositary institution; Certainly, this bacterial strain also can obtain from above-mentioned preservation mechanism; For guaranteeing technique effect, it is possible to adopt the bacterial strain that other are of the same race;
In following embodiment, adopted alkaline protease is the commercial alkaline protease finished product for 200U/mg alive of a kind of enzyme purchased from Amresco company; What certainly may be used without other companies has the alkaline protease finished product that similar enzyme is lived;
The raw materials such as corn syrup (DE value, 38 ~ 42%) involved in following embodiment, arabic gum and maltodextrin (DE value, 13 ~ 17%) are food stage, purchased from Henan Ming Rui food additive company;
In following embodiment, involved raw tobacco material has: Xuchang beating and double roasting CJ91 module Nicotiana tabacum Lves in 2014 and Xuchang DBK54 beating and double roasting module Nicotiana tabacum L. in 2014, provides by the relevant unit of China Tobacco Henan Industrial Co., Ltd belonging to applicant; Nicotiana tabacum L. preservation warehouse is the inner paving tobacco leaf warehouses of ten belonging to China Tobacco Henan Industrial Co., Ltd, and Nicotiana tabacum L. preserves under field conditions (factors), and moisture content in leaves content is about 11% ~ 13%.
It is further to note that the detection of thalline quantity adopts pour plate counting method in bacterium solution.
It should be noted that, tabacum sensory evaluation adopts 16 specialties belonging to China Tobacco Henan Industrial Co., Ltd, that randomly draw personnel that smoke panel test with reference to " in Henan cigarette single-tobacco-typed cigarette sensory quality assessment method ", the aesthetic quality of Nicotiana tabacum L. to be evaluated; Concrete operations are:
By tobacco leaf chopping, make the cigarette sample of cigarette specification (20+64) × 24.5mm, screen through quality, 22 DEG C ± 1 DEG C, the climatic chamber of relative humidity 60 ± 2% is smoked panel test after balance 48h;
Organoleptic indicator includes aroma quality, perfume quantity, concentration, soft and fine degree, pleasant impression, miscellaneous QI, zest, strength, flammability, Lycoperdon polymorphum Vitt etc., each index, all by 9 points of system marking, is then added summation by different weight coefficient (referring to following table) conversions and obtains sense organ total score;
The weight coefficient table of every organoleptic indicator is as follows:
Embodiment 1
The composite biological agent microcapsule for improving tabacum sensory that the present embodiment provides, is prepared from as follows.
(1) core is prepared:
Bacillus subtilis bacterium solution is prepared as follows forming: takes bacillus subtilis strain, LB fluid medium, 30 DEG C, the cultivation amplification of 200rpm shaking table, with aseptic culture fluid for blank, records the bacterium solution absorbance OD at 600nm place600, work as OD600=6.0, as application microbial inoculum, now counting shows that in bacterium solution, thalline quantity=7,000,000,000/mL(is specially 7.02 × 109Cfu/mL);
Sphingol single-cell bacterium solution is prepared as follows forming: takes Sphingomonas, inorganic salt liquid culture medium, 30 DEG C, the cultivation amplification of 200rpm shaking table, with aseptic culture fluid for blank, records the absorbance OD at 600nm place of bacterium solution600, work as OD600=2.0, as application microbial inoculum, now counting shows that in bacterium solution, thalline quantity=5,300,000,000/mL(is specially 5.31 × 109Cfu/mL);
Every liter of inorganic salt liquid culture medium contains: chlorogenic acid 0.2g; Na2HPO4·12H2O6.15g; KH2PO41.52g; (NH4)2SO41.5g; MgSO4·7H2O0.20g; CaCl2·2H2O0.05g;
The enzyme of the alkaline protease a kind of enzyme for the Amresco company alive alkaline protease commercially produced product for 200U/mg alive;
Bacillus subtilis bacterium solution, Sphingol single-cell bacterium solution, alkaline protease are prepared respectively, mix in proportion before preparing composite biological agent microcapsule again.
(2) wall material is prepared:
Corn syrup, arabic gum and maltodextrin are mixed in proportion, prepares into solution that mass concentration is 40% as wall material solution;
By quality ratio, corn syrup: arabic gum: maltodextrin=3:5:4.
(3) composite biological agent microcapsule is prepared:
Take wall material solution in step (2), keep 600r/min high-speed stirred, slowly add the core of mix homogeneously in step (1) in proportion, after interpolation, continue stirring 30min and guarantee mix homogeneously, as material solution; By quality ratio, wall material: core=1:1;
By this material solution 60 DEG C, 40MPa when high pressure homogenizing, form stable feed liquid;
The stable feed liquid formed is carried out spray drying in spray drying tower, and design parameter is: spraying feeding temperature is 60 DEG C, and feed rate is 150mL/min, and inlet temperature is 175 DEG C, and leaving air temp is 60 DEG C;
Final gained powder-product is the composite biological agent microcapsule in the application.
Prepared composite biological agent microcapsule is carried out microexamination; its structure is as shown in Figure 1; what viable bacteria was comparatively concentrated is gathered in the inside of microcapsule thus forming core A; and outer wrap part viable bacteria is less; it is mainly the wall material B that corn syrup, arabic gum and maltodextrin are formed; core A is formed protective effect by wall material B mono-aspect, provides certain nutrient substance on the one hand, so that the viable bacteria in core A can survive the long period.
With bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: the wall material of enzyme=0.7:0.7:0.8 as a comparison case, is designated as mixed biologic microbial inoculum HJY-0, and detection shows, viable count=6.01 × 109Cfu/mL;
With bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: the composite biological agent microcapsule prepared by wall material of enzyme=0.7:0.7:0.8, being designated as microcapsule HJY-1, detection shows, this capsule grain diameter is 20 ~ 60 μm, viable bacteria is gathered in capsule core position, viable count=5.77 × 109Cfu/mL;
With bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: the composite biological agent microcapsule prepared by wall material of enzyme=0.8:1.0:1.2, being designated as microcapsule HJY-2, detection shows, this capsule grain diameter is 30 ~ 60 μm, viable bacteria is gathered in capsule core position, viable count=5.17 × 109cfu/mL。
The survival condition of the thalline in above-mentioned prepared HJY-0, HJY-1, HJY-2 is detected, and related experiment is briefly discussed below.
HJY-0, HJY-1, HJY-2 are respectively placed in 25 DEG C, the constant temperature and humidity of relative humidity 65% when storage, sampled every two months, detect viable count, calculating survival rate compared with initial value, investigate thalline number change situation. Concrete testing result is as shown in the table:
As can be seen from the above table, mixed biologic microbial inoculum HJY-0 is after depositing 5 months, thalline quantity has been reduced to about initial value half, and after being processed by micro encapsulation, even if after depositing 8 months, thalline quantity is still higher, thus for accelerating tobacco mellowing, continuing to improve quality of tobacco and all have the probability of application.
Embodiment 2
After mixing with Nicotiana tabacum L. respectively by a certain percentage with HJY-0, HJY-1, the HJY-2 prepared by embodiment 1, being still placed in tobacco warehouse by Nicotiana tabacum L. and deposit, Nicotiana tabacum L. deposits period, samples every two months, and Nicotiana tabacum L. after processing is evaluated. In different disposal situation, the quality comparison briefing of Nicotiana tabacum L. is as follows.
Situation of change without the quality of tobacco of microbial inoculum (blank) is as shown in the table:
。
When HJY-0 processes Nicotiana tabacum L., processing by 1.0% interpolation of quality of tobacco, after process, quality of tobacco situation of change is as shown in the table:
。
When HJY-1 processes Nicotiana tabacum L., being added in the ratio of the 2.0% of quality of tobacco, after process, quality of tobacco situation of change is as shown in the table:
。
When HJY-1 processes Nicotiana tabacum L. simultaneously, being added processing by the different proportion of quality of tobacco, when processing 5 months, sensory scores's situation of quality of tobacco is as shown in the table:
Can substantially finding out from above-mentioned contrast, when directly adopting microbial bacterial agent to process, at the initial stage of process, it is comparatively obvious that quality of tobacco improves effect, but prolongation over time, it is extremely limited that quality of tobacco improves effect. And when adopting composite biological agent microcapsule provided herein to process, quality of tobacco improves then comparatively steady, prolongation over time, quality of tobacco can continue to be improved, and improves effect also due to directly adopt microbial bacterial agent to process; Further for improving effect, when the addition of composite biological agent microcapsule is less than 2.5%, the improvement effect of quality of tobacco all can be comparatively obviously improved.
When HJY-2 processes Nicotiana tabacum L., being added in the ratio of the 2.2% of quality of tobacco, after process, quality of tobacco situation of change is as shown in the table:
As can be seen from the above table, for the composite biological agent microcapsule of different microorganisms ratio, when identical addition is with the identical resting period, after HJY-1 process, Nicotiana tabacum L. improves effect and is better than HJY-2 treatment effect, thus HJY-1 is preferred embodiment.
Claims (5)
1. the composite biological agent microcapsule being used for improving tabacum sensory, it is characterised in that this composite biological agent microcapsule is prepared from by spray drying by core and the wall material of mix homogeneously;
Described core is the mixture of bacillus subtilis bacterium solution, Sphingol single-cell bacterium solution and alkaline protease, wherein in bacillus subtilis bacterium solution, thalline quantity is 40 ~ 6,000,000,000/mL, in Sphingol single-cell bacterium solution, thalline quantity is 10 ~ 3,000,000,000/mL, and described alkaline protease enzyme is lived as 100-400U/mg; By quality ratio, bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: enzyme=0.8 ~ 1.0:0.8 ~ 1.0:1.0 ~ 1.2;
Described wall material is the mixed liquor that mass concentration is 30 ~ 50% that corn syrup, arabic gum and maltodextrin mix, by quality ratio, in mixed liquor, and corn syrup: arabic gum: maltodextrin=3:5:4.
2. as claimed in claim 1 for improving the composite biological agent microcapsule of tabacum sensory, it is characterised in that in core by quality ratio, bacillus subtilis bacterium solution: Sphingol single-cell bacterium solution: enzyme=1.0:1.0:1.2.
3. as claimed in claim 1 for improving the composite biological agent microcapsule of tabacum sensory, it is characterised in that by quality ratio, in composite biological agent microcapsule, core: wall material=1:1.
4. it is used for the preparation method improving the composite biological agent microcapsule of tabacum sensory described in claim 1, it is characterised in that specifically include following steps:
(1) core is prepared:
Bacillus subtilis bacterium solution is prepared as follows forming: taking bacillus subtilis strain, LB fluid medium, 30 DEG C, the cultivation amplification of 200rpm shaking table, when in bacterium solution, thalline quantity reaches 40 ~ 6,000,000,000/mL, as application microbial inoculum;
Sphingol single-cell bacterium solution is prepared as follows forming: taking Sphingomonas, inorganic salt liquid culture medium, 30 DEG C, the cultivation amplification of 200rpm shaking table, when in bacterium solution, thalline quantity reaches 10 ~ 3,000,000,000/mL, as application microbial inoculum;
Described inorganic salt liquid culture medium, contains in concrete every liter of culture medium: chlorogenic acid 0.2g; Na2HPO4·12H2O6.15g; KH2PO41.52g; (NH4)2SO41.5g; MgSO4·7H2O0.20g; CaCl2·2H2O0.05g;
Bacillus subtilis bacterium solution, Sphingol single-cell bacterium solution, alkaline protease are prepared respectively, prepare mix homogeneously before composite biological agent microcapsule;
(2) wall material is prepared:
Corn syrup, arabic gum and maltodextrin are mixed in proportion, prepares into solution that mass concentration is 30 ~ 50% as wall material solution;
(3) composite biological agent microcapsule is prepared:
Take wall material solution in step (2), under stirring condition, be proportionally added in step (1) core of mix homogeneously, as material solution after mix homogeneously;
By this material solution 40 ~ 60 DEG C, 30 ~ 40MPa when high pressure homogenizing, form stable feed liquid;
The stable feed liquid formed is carried out spray drying in spray drying tower, and design parameter is: spraying feeding temperature is 50 DEG C ~ 60 DEG C, and feed rate is 130 ~ 150mL/min, and inlet temperature is 170 DEG C ~ 180 DEG C, and leaving air temp is 50 DEG C ~ 60 DEG C;
Final gained powder-product is the composite biological agent microcapsule in the application.
5. for improving the application in tobacco mellowing of the composite biological agent microcapsule of tabacum sensory described in claim 1, it is characterised in that by composite biological agent microcapsule in less than quality of tobacco 2.5% ratio mix.
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| CN111642788A (en) * | 2020-05-11 | 2020-09-11 | 重庆中烟工业有限责任公司 | Biological preparation capsule containing bacillus cereus |
| CN112316862A (en) * | 2020-11-25 | 2021-02-05 | 江西中烟工业有限责任公司 | Preparation method of stevia rebaudiana enzyme microcapsule and application of stevia rebaudiana enzyme microcapsule in cigarettes |
| CN113383981A (en) * | 2021-05-28 | 2021-09-14 | 天昌国际烟草有限公司 | Complex enzyme preparation for improving tobacco leaf smoking quality of Yu-produced tobacco and application of complex enzyme preparation in threshing and redrying process |
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| CN113383981A (en) * | 2021-05-28 | 2021-09-14 | 天昌国际烟草有限公司 | Complex enzyme preparation for improving tobacco leaf smoking quality of Yu-produced tobacco and application of complex enzyme preparation in threshing and redrying process |
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