CN103443266A - 在解脂耶氏酵母中使用啤酒糖酵母suc2基因以利用蔗糖 - Google Patents

在解脂耶氏酵母中使用啤酒糖酵母suc2基因以利用蔗糖 Download PDF

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CN103443266A
CN103443266A CN2011800630477A CN201180063047A CN103443266A CN 103443266 A CN103443266 A CN 103443266A CN 2011800630477 A CN2011800630477 A CN 2011800630477A CN 201180063047 A CN201180063047 A CN 201180063047A CN 103443266 A CN103443266 A CN 103443266A
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yarrowia lipolytica
sucrose
seq
xpr2
invertase
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Chinese (zh)
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S-P.洪
J.E.塞普
Q.Q.朱
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Physics & Mathematics (AREA)
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  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2011800630477A 2010-12-30 2011-11-15 在解脂耶氏酵母中使用啤酒糖酵母suc2基因以利用蔗糖 Pending CN103443266A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201061428590P 2010-12-30 2010-12-30
US61/428,590 2010-12-30
PCT/US2011/060788 WO2012091812A1 (en) 2010-12-30 2011-11-15 Use of saccharomyces cerevisiae suc2 gene in yarrowia lipolytica for sucrose utilization

Publications (1)

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CN103443266A true CN103443266A (zh) 2013-12-11

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CN2011800630477A Pending CN103443266A (zh) 2010-12-30 2011-11-15 在解脂耶氏酵母中使用啤酒糖酵母suc2基因以利用蔗糖

Country Status (10)

Country Link
US (1) US8735137B2 (https=)
EP (1) EP2658962A1 (https=)
JP (1) JP2014503216A (https=)
KR (1) KR20140000711A (https=)
CN (1) CN103443266A (https=)
AU (1) AU2011353002A1 (https=)
BR (1) BR112013016769A2 (https=)
CA (1) CA2824584A1 (https=)
CL (1) CL2013001887A1 (https=)
WO (1) WO2012091812A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104178435A (zh) * 2014-08-21 2014-12-03 天津科技大学 一种适合于高糖面团发酵的高活性干酵母
CN107022499A (zh) * 2017-05-04 2017-08-08 北京化工大学 调控酿酒酵母社会行为提高生物乙醇发酵速率效率的方法
CN119824045A (zh) * 2025-03-19 2025-04-15 东晓生物科技股份有限公司 Fads基因在调控解脂耶氏酵母发酵生产赤藓糖醇的产量中的应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3004177A1 (en) 2015-11-24 2017-06-01 Cargill, Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
CN108070579A (zh) * 2017-12-21 2018-05-25 中诺生物科技发展江苏有限公司 一种蔗糖转化酶工业化生产方法
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法
US20250320535A1 (en) 2021-05-17 2025-10-16 Dsm Ip Assets B.V. Novel technology to enable sucrose utilization in strains for biosynthetic production
DK181442B1 (en) * 2021-05-17 2024-01-17 Dsm Ip Assets Bv Cells capable of producing one or more human milk oligosaccharides (hmos) comprising expression of an invertase, method comprising said cells for biosynthetic production and use of said cells for biosynthetic production; use of an invertase in a biosynthetic production

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104178435A (zh) * 2014-08-21 2014-12-03 天津科技大学 一种适合于高糖面团发酵的高活性干酵母
CN107022499A (zh) * 2017-05-04 2017-08-08 北京化工大学 调控酿酒酵母社会行为提高生物乙醇发酵速率效率的方法
CN119824045A (zh) * 2025-03-19 2025-04-15 东晓生物科技股份有限公司 Fads基因在调控解脂耶氏酵母发酵生产赤藓糖醇的产量中的应用

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CL2013001887A1 (es) 2014-01-31
US20120171719A1 (en) 2012-07-05
EP2658962A1 (en) 2013-11-06
KR20140000711A (ko) 2014-01-03
WO2012091812A1 (en) 2012-07-05
CA2824584A1 (en) 2012-07-05
BR112013016769A2 (pt) 2016-10-11
AU2011353002A1 (en) 2013-05-30
JP2014503216A (ja) 2014-02-13
US8735137B2 (en) 2014-05-27

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Application publication date: 20131211