CN103440807A - Method for preparing green goose torso bone and heart-lung cyst cavity connected colored specimen - Google Patents

Method for preparing green goose torso bone and heart-lung cyst cavity connected colored specimen Download PDF

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Publication number
CN103440807A
CN103440807A CN2013104049563A CN201310404956A CN103440807A CN 103440807 A CN103440807 A CN 103440807A CN 2013104049563 A CN2013104049563 A CN 2013104049563A CN 201310404956 A CN201310404956 A CN 201310404956A CN 103440807 A CN103440807 A CN 103440807A
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China
Prior art keywords
goose
cardiopulmonary
preparation
blister cavities
green goose
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Pending
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CN2013104049563A
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Chinese (zh)
Inventor
王荣林
葛兆宏
蒋春茂
贺生中
张步彩
王兔林
王康
韩青平
王加乐
李乃竹
胡新岗
程汉
魏宁
李巨银
李芙蓉
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Jiangsu Agri Animal Husbandry Vocational College
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王荣林
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Priority to CN2013104049563A priority Critical patent/CN103440807A/en
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Abstract

The invention relates to a method for preparing a green goose torso bone and heart-lung cyst cavity connected colored specimen, for providing solutions to technical problems, i.e., a method of preparing a heart-lung cyst cavity coloring combination liquid by using such materials as waste table tennis balls, waterless ethanol, butanone, a proper amount of oil painting pigments and the like, and a method for preparing a torso bone alkaline etching chlorination combination liquid by using such materials as sodium hydroxide, waterless ethanol, calcium hypochlorite, calcium hydrate and the like. The provided method comprises: selecting a live green goose which is medium-sized in body figure and robust and lossless in health; performing bloodletting on the mouth of the green goose until death, cleaning, and fixing the goose to a pipe cavity; performing coloring treatment on the heart-lung cyst cavity of the green goose; placing ethanol in a vertebra gap and a joint cavity for corrosion prevention; hardening and fixing materials, and peeling and picking meat; performing preparation and treatment on a bone alkaline etching combination liquid; and washing with water, repairing, installing a support and preserving with a transparent cover so as to prepare the specimen having the same heart-lung cyst cavity organ as a real live goose. By using the provided method, the needs of animal husbandry and veterinary specialties for animal dissecting and livestock and poultry production, the needs of agriculture and husbandry colleges for teaching and practice, and the needs of scientific research institutions and the like are well met, and the specimen can be stored on a shelf at a normal temperature for a long time with color and without unfavorable flavor.

Description

The preparation method of a kind of green goose skeleton trunci and the coloured sample of cardiopulmonary blister cavities disjunctor
Technical field
The present invention relates to a kind of animal skeleton and internal organs sample technology of preparing, particularly relate to the preparation method of a kind of green goose skeleton trunci and the coloured sample of cardiopulmonary blister cavities disjunctor.
Background technology
In current domestic institution of higher learning pluck organ teaching specimen teaching aid, most organ samples that also are confined to the chemical reagent immersion, especially the most common with formalin infuse encapsulation sample, but in actual applications, formalin humid preparation shortcoming is more than advantage.Make the advantages such as simple, that the cost of material is low, material antiseptic is effective as the formalin humid preparation has, but the formalin humid preparation have formaldehyde odor smoke the people, carcinogenic, pollute teaching environment, corrode arm skin and affect health of people, use the generally acknowledged shortcomings such as the pale colourless stiff and teaching efficiency of inconvenient, sample is poor.By the processing that colors in of green goose cardiopulmonary blister cavities disjunctor, can change the pale colourless stiff defect of formalin humid preparation.And green goose skeleton trunci and cardiopulmonary blister cavities disjunctor are treated as the coloured sample of dry finished product, thoroughly change formalin humid preparation smell and smoke the people, corrode arm skin and affect health of people, use and get and deposit inconvenience and the defect such as teaching efficiency is poor.
Summary of the invention
The present invention seeks to save as by green goose skeleton trunci and the processing of cardiopulmonary blister cavities disjunctor, dress support and translucent cover preparation method's modification of the coloured sample of dry finished product, to strengthen the photochromic and directly perceived sense of sample, make it to become the teaching specimen that has several colors and nothing to smoke people's smell, make it neither to need chemical reagent soak to be preserved, having again teaching get deposit convenient to both and on health without a kind of green goose skeleton trunci of impact and the preparation method of the coloured sample of cardiopulmonary blister cavities disjunctor.Each organ morphology anatomic teaching and the poultry diease teaching features such as kinematic system, Cardiovascular System, digestive system, respiratory system that can show green goose skeleton trunci and the coloured sample of cardiopulmonary blister cavities disjunctor, for teaching research and practice are offered help.
For achieving the above object, technical scheme proposed by the invention is: the preparation method of a kind of green goose skeleton trunci and the coloured sample of cardiopulmonary blister cavities disjunctor comprises the following steps realization:
(1) select the fresh harmless green goose alive of the medium stalwartness of build (2 kilograms of body weight) material;
(2) clean pouring blood with the goose left ventricular cannulation with anticorrosion anti-freezing liquid by blood vessel bloodletting in the goose oral cavity is lethal;
(3) processing that colors in green goose cardiopulmonary blister cavities is filled with red filling agent low, high concentration in the right ventricle intubate.Fill with respectively blue filling agent low, high concentration in left ventricle, wing vein (after-teeming), femoral vein (after-teeming) intubate.Fill with respectively yellow filling agent low, high concentration again in tracheae and oesophagus intubate with wet cloth plug otch.And in from the slotting lead bullion silk support of green goose oesophagus crop mouth to the body cavity glandular stomach, filling agent is fixed it;
(4) anticorrosion fixing to injecting absolute ethyl alcohol in green goose intervertebral space and joint blister cavities, wrap up and guaranteeing the quality with infiltrating straight alcohol wet gauze and plastic sheeting at the skin place that has of gooseneck boom and goose toe;
(5) sclerosis in water tank that salts down of goose material integer is fixed 10~20 hours, removes by skin and picks residual clearly meat;
(6) preparation of bone alkaline etching combination liquid is processed, by containing proper proportions such as NaOH, absolute ethyl alcohol, calcium hypochlorite, calcium hydroxide, water, mixing in alkaline etching liquid and flood material 1~3 day, costal cartilage is coated to vaseline, spill a little light ashs to dark soft tissue and flood again material 3~5 days.After corrosion repeatedly washing totally make bone and bleached, the bone ligament intact with cardiopulmonary blister cavities disjunctor after finished product be all coloured dry finished product.
(7) heat shaping with well heater, prune unnecessary filling material, repair form, dress support and translucent cover and preserve at the adhesion place of fractureing.
The technique effect that adopts technique scheme to reach is: resulting green goose skeleton trunci and each position Morophological And Anatomical of the coloured sample of cardiopulmonary blister cavities disjunctor are identical with cardiopulmonary blister cavities connected device official morphologic localization with the true bone of live body.Preparation method's modification that the preparation processing, dress support and the translucent cover that pick meat, bone alkaline etching liquid save as the coloured sample of dry finished product is fixed and is removed the peel in the processing that colors in green goose cardiopulmonary blister cavities, material sclerosis, to strengthen the photochromic and directly perceived sense of sample, make it to become the teaching specimen that has several colors and nothing to smoke people's smell, make it neither to need chemical reagent soak to be preserved, having again teaching get deposit convenient to both and on health animal skeleton and the organ sample preparation method without impact.Each organ morphology anatomic teaching and the poultry diease teaching features such as kinematic system, Cardiovascular System, digestive system, respiratory system that can show green goose skeleton trunci and the coloured sample of cardiopulmonary blister cavities disjunctor, for teaching research and practice are offered help.
Further, the emphasis green goose cardiopulmonary blister cavities finished product of moulding sclerosis after fixing that color in is coloured exsiccata finished product in described step (3).
Further, color in emphasis green goose cardiopulmonary blister cavities in described step (3) and combine the liquid preparation and fill:
1. the preparation of combination liquid colors in: containing 4~8 milliliters of absolute ethyl alcohols, contain 1.5~3 milliliters of butanone, oil colour in every gram (waste product recycling) table tennis: appropriate.Airing after 2 hours films that discolor of ethanol bubble for the table tennis that will give up section, mix after dissolving agglutination and add appropriate pigment molten even standby in above prescription ratio.
2. fill in the cardiopulmonary blister cavities: to the 80% ethanol liquaemin salt solution green goose left ventricle for (that is: in 100 parts of anticorrosion anti-freezing liquids, containing 80 parts of straight alcohols, containing 20 parts of liquaemin salt solution), pouring blood is cleaned in anticorrosion anti-freezing.The red filling agent of low from filling with in the right ventricle intubate (9% table tennis color in agent), high concentration (18% table tennis color in agent), the blue filling agent of low from filling with respectively in left ventricle, wing vein (after-teeming), femoral vein (after-teeming) intubate (9% table tennis color in agent), high concentration (18% table tennis color in agent).The yellow filling agent of low from filling with respectively in tracheae and oesophagus intubate (9% table tennis color in agent gradation fill with branch peripheral blood tube chamber), high concentration (18% table tennis color in agent gradation fill with the dry lumen of vessels of major branch) again.
Further, in described step (6), the finished product after emphasis green goose bone and the making of cardiopulmonary blister cavities disjunctor is coloured dry finished product sample.
Further, in described step (6), preparation and the alkaline etching of emphasis alkaline etching combination liquid are processed:
1. the preparation of bone alkaline etching combination liquid: in every 100 milliliters of alkaline etching liquid, containing 45 gram NaOH, containing 5 milliliters of absolute ethyl alcohols, containing 3~4 gram calcium hypochlorites, containing 0.15 gram calcium hydroxide, containing 47 ml waters, moltenly in proportion all become alkaline etching combination liquid standby.
2. alkaline etching is processed: flood material 1~3 day by bone alkaline etching combination liquid configuration proportion mixing liquid, costal cartilage is coated to vaseline, spill a little light ashs to soft deeply again tissue and flood material 3~5 days again.After corrosion repeatedly water clean into bone and bleached, the bone ligament intact with cardiopulmonary blister cavities disjunctor after finished product be all coloured dry finished product.Be well positioned to meet herd doctor's class specialty zootomy, Production of Livestock and Poultry, agriculture and animal husbandry college teaching and put into practice, the needs such as scientific research institution, show each organ morphology anatomic teaching features such as kinematic system, cardiovascular system, digestive system, respiratory system, also offer help for clinical poultry diease practical teaching and scientific research.
Embodiment
Gain knowledge us from zootomy, there is no diaphram between the green goose splanchnocoel, the airbag chamber that several prosperities are inside arranged, splanchnocoel back cardiopulmonary kidney organ's morphologic localization has been compared similarities and differences part with mammal, and respiratory apparatus, digestive organs and the urogenital organ important organ of susceptible disease that is the animal common disorder, the vitals that the multiple course teaching such as it and Zootomy, the inside and outside obstetrics of animal, poultry disease prevention and control and veterinary clinic Clinics and practical studies interrelate.Fresh condemned MAT'L after the useless table tennis that we utilize the student to collect and vivisection teaching, processing, the washing of the processing that colors in green goose cardiopulmonary blister cavities, bone alkaline etching combination liquid are shelved and saved as the coloured sample of dry finished product, repeatedly be applied to the animal practical teaching throughout the year, teaching efficiency significantly improves, for the teaching of aquatic bird industry is offered help with research.The embodiment of concrete operations of the present invention is completed by following steps:
The first step, select green goose alive (2 kilograms of the body weight) material of the medium robust lossless of build.
Second step, make goose lethal two vascellum laterale bloodletting in the green goose oral cavity, from left ventricular cannulation in the left abdomen lower breast of goose intercostal space and clean pouring blood with anticorrosion anti-freezing liquid and be fixed.
The 3rd step, the preparation of combination liquid and filling color in green goose cardiopulmonary blister cavities:
1. the preparation of combination liquid colors in: containing 4~8 milliliters of absolute ethyl alcohols, contain 1.5~3 milliliters of butanone, oil colour in every gram (waste product recycling) table tennis: appropriate.Airing after 2 hours films that discolor of ethanol bubble for the table tennis that will give up section, mix after dissolving agglutination and add appropriate pigment molten even standby in above prescription ratio.
2. fill in the cardiopulmonary blister cavities: to the 80% ethanol liquaemin salt solution green goose left ventricle for (that is: in 100 parts of anticorrosion anti-freezing liquids, containing 80 parts of straight alcohols, containing 20 parts of liquaemin salt solution), pouring blood is cleaned in anticorrosion anti-freezing.The red filling agent of low from filling with in the right ventricle intubate (9% table tennis color in agent), high concentration (18% table tennis color in agent), the blue filling agent of low from filling with respectively in left ventricle, wing vein (after-teeming), femoral vein (after-teeming) intubate (9% table tennis color in agent), high concentration (18% table tennis color in agent).The yellow filling agent of low from filling with respectively in tracheae and oesophagus intubate (9% table tennis color in agent gradation fill with branch peripheral blood tube chamber), high concentration (18% table tennis color in agent gradation fill with the dry lumen of vessels of major branch) again.
The 4th step, anticorrosion fixing to injecting absolute ethyl alcohol in green goose intervertebral space and joint blister cavities, wrap up and guaranteeing the quality with infiltrating straight alcohol wet gauze and plastic sheeting at the skin place that has of gooseneck boom and goose toe.
The 5th step, fix 10~20 hours by the sclerosis in water tank that salts down of goose material integer, removes that to be picked residual clearly meat by skin stand-by.
The 6th step, preparation and the alkaline etching of alkaline etching combination liquid are processed:
1. the preparation of bone alkaline etching combination liquid: in every 100 milliliters of alkaline etching liquid, containing 45 gram NaOH, containing 5 milliliters of absolute ethyl alcohols, containing 3~4 gram calcium hypochlorites, containing 0.15 gram calcium hydroxide, containing 47 ml waters, moltenly in proportion all become alkaline etching combination liquid standby.
2. alkaline etching is processed: the ratio mixing liquid in the preparation of bone alkaline etching combination liquid floods material 1~3 day, and costal cartilage is coated to vaseline, spills a little light ashs to deeply again soft tissue and floods material 3~5 days again.After corrosion repeatedly water clean into bone and bleached, the bone ligament intact with cardiopulmonary blister cavities disjunctor after finished product be all coloured dry finished product.Be well positioned to meet herd doctor's class specialty zootomy, Production of Livestock and Poultry, agriculture and animal husbandry college teaching and put into practice, the needs such as scientific research institution, show each organ morphology anatomic teaching features such as kinematic system, cardiovascular system, digestive system, respiratory system, also offer help for clinical poultry diease practical teaching and scientific research.
The 7th step, with well heater heat shaping, repair form, goose front mouth galvanized wire support is fixedly mounted on respectively on goose sample base plate with bipod and the translucent cover preservation.

Claims (5)

1. the preparation method of a green goose skeleton trunci and the coloured sample of cardiopulmonary blister cavities disjunctor comprises the following steps:
(1) select the green goose alive (2 kilograms of body weight) of the medium robust lossless of build; (2) two vascellum laterale bloodletting in the green goose oral cavity are lethal fixing with the tube chamber cleaning; (3) processing that colors in green goose cardiopulmonary blister cavities, preparation and the filling of the combination liquid that it is characterized in that coloring in; (4) anticorrosion to injecting straight alcohol in intervertebral space and articular cavity; (5) sclerosis of material integer is fixing picks meat with peeling; (6) preparation of bone alkaline etching combination liquid is processed, and it is characterized in that preparation and the alkaline etching of alkaline etching combination liquid processed; (7) washing, repairing, dress support and translucent cover are preserved.
2. the coloured sample preparation method of green goose skeleton trunci and cardiopulmonary blister cavities disjunctor as claimed in claim 1, it is characterized in that: the rear finished product that colors in emphasis cardiopulmonary blister cavities in described step (3) is coloured dry finished product.
3. the coloured sample preparation of green goose skeleton trunci as claimed in claim 1 and cardiopulmonary blister cavities disjunctor, it is characterized in that: the liquid that colors in described step (3) preparation and filling: contain 4~8 milliliters of absolute ethyl alcohols in every gram (waste product recycling) table tennis, containing 1.5~3 milliliters of butanone, appropriate oil colour, mix after dissolving agglutination and add appropriate pigment molten even standby in above prescription ratio.The goose left ventricle is cleaned and drenched blood with the anticorrosion anti-freezing of 80% ethanol heparin saline, fill with red filling agent low, high concentration in the right ventricle intubate, fill with respectively blue filling agent low, high concentration in left ventricle, wing vein (after-teeming), femoral vein (after-teeming) intubate, in tracheae and oesophagus intubate, fill with respectively yellow filling agent low, high concentration.
4. the coloured sample preparation method of green goose skeleton trunci and cardiopulmonary blister cavities disjunctor as claimed in claim 1, it is characterized in that: in described step (6), emphasis bone and cardiopulmonary blister cavities disjunctor finished product are coloured dry finished products.
5. the coloured sample preparation method of green goose skeleton trunci and cardiopulmonary blister cavities disjunctor as claimed in claim 1, it is characterized in that: in described step (6), the preparation of emphasis alkaline etching combination liquid is processed with alkaline etching: in every 100 milliliters of alkaline etching liquid, contain 45 gram NaOH; Containing 5 milliliters of absolute ethyl alcohols, containing 3~4 gram calcium hypochlorites, containing 0.15 gram calcium hydroxide, containing 47 ml waters, in above ratio mixing liquid, flood material 1~3 day, costal cartilage is coated to vaseline, spill a little light ashs to dark soft tissue and flood again material 3~5 days.Repeatedly wash into bone after corrosion and bleached, the bone ligament intact with cardiopulmonary blister cavities disjunctor after finished product be coloured dry finished product.
CN2013104049563A 2013-08-30 2013-08-30 Method for preparing green goose torso bone and heart-lung cyst cavity connected colored specimen Pending CN103440807A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104794974A (en) * 2015-04-20 2015-07-22 江苏农牧科技职业学院 Dry specimen of duck-organ multicolor molding spread skeleton wing and preparation method of dry specimen
CN106070183A (en) * 2016-08-25 2016-11-09 卞陆杰 The manufacture method of zoological specimens

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CN101996512A (en) * 2010-11-19 2011-03-30 新乡医学院 Method for displaying animal (bovine) heart conducting system

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104794974A (en) * 2015-04-20 2015-07-22 江苏农牧科技职业学院 Dry specimen of duck-organ multicolor molding spread skeleton wing and preparation method of dry specimen
CN106070183A (en) * 2016-08-25 2016-11-09 卞陆杰 The manufacture method of zoological specimens

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