CN110367157A - Variegated sculpins artificial fecundation method - Google Patents
Variegated sculpins artificial fecundation method Download PDFInfo
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- CN110367157A CN110367157A CN201910823114.9A CN201910823114A CN110367157A CN 110367157 A CN110367157 A CN 110367157A CN 201910823114 A CN201910823114 A CN 201910823114A CN 110367157 A CN110367157 A CN 110367157A
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- 241000276573 Cottidae Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 114
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 44
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 44
- 210000004681 ovum Anatomy 0.000 claims abstract description 41
- 230000012447 hatching Effects 0.000 claims abstract description 19
- 235000013601 eggs Nutrition 0.000 claims description 23
- 239000000644 isotonic solution Substances 0.000 claims description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 13
- 229910052760 oxygen Inorganic materials 0.000 claims description 13
- 239000001301 oxygen Substances 0.000 claims description 13
- LVDKZNITIUWNER-UHFFFAOYSA-N Bronopol Chemical compound OCC(Br)(CO)[N+]([O-])=O LVDKZNITIUWNER-UHFFFAOYSA-N 0.000 claims description 9
- 229960003168 bronopol Drugs 0.000 claims description 9
- 210000000582 semen Anatomy 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 240000005373 Panax quinquefolius Species 0.000 claims description 7
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 7
- 239000011152 fibreglass Substances 0.000 claims description 7
- 241001247197 Cephalocarida Species 0.000 claims description 5
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 claims description 4
- 229920000153 Povidone-iodine Polymers 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 229960001621 povidone-iodine Drugs 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000004448 titration Methods 0.000 claims description 3
- 239000012267 brine Substances 0.000 claims description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 2
- 241000251468 Actinopterygii Species 0.000 abstract description 18
- 230000004720 fertilization Effects 0.000 abstract description 4
- 230000002159 abnormal effect Effects 0.000 abstract description 3
- 230000036541 health Effects 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 240000002234 Allium sativum Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001529596 Pontinus kuhlii Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000004280 healthy diet Nutrition 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
Variegated sculpins artificial fecundation method, belongs to fish reproduction technical field, the environmental condition, condition of water quality including artificial propagation and the operation requirement to reproduction technique.The present invention realizes the artificial propagation of variegated sculpins; it adopts ovum rate 100%; rate of fertilization 90%; send out eye rate 93%; hatching rate 85%, abnormal rate 0.5 ‰ make it possible variegated sculpins biological control; new rare cultured fishes are provided for cold water fish industry, and then are pushed cold water fish industry health, efficiently developed.
Description
Technical field
The invention belongs to fish reproduction technical fields, especially relate to variegated sculpins artificial fecundation method.
Background technique
Variegated sculpins is subordinate to Rockfish shape mesh Cottoidei Cottidae sculpins category, China be only distributed in Heilungkiang,
Song Hua River, the Wusuli River, the Suifenhe, TUMENJIANG RIVER, the Yalu River, Liaohe River tributary Hun River, Taizihe River, Liaodong Peninsula Bi Liuhe etc., state
NORTHERN KOREA, all states in Far-east Area of Russia and Northern Europe are seen outside, and 2 rheological properties are mature, are with aquatic insect and its larva, small fish and shrimp
Food.Variegated sculpins is the rare fish of small-sized cold water, and outstanding feature is: (1) fine and tender taste, is in garlic clove shape, in good taste, nutrition
Value is high.(2) in pollution-free, water quality clear brook, living environment is environmentally protective, is typical green food, meets for existence
Demand of the current consumer to healthy diet, green food, quality are excellent.(3) it is quite received by the market, and the source of goods is in great shortage, market is sold
Valence is up to 100~200 yuan/kilogram, and economic value is high, great Development volue.Currently, domestic be also only limitted to about variegated sculpins
Biology Primary Study, and length is brief, still belongs to blank to its resource, breeding and the special item of development and utilization, therefore existing
Technology needs the new technical solution of one kind to solve the problems, such as this.
Summary of the invention
The technical problems to be solved by the present invention are: providing variegated sculpins artificial fecundation method, sculpins is realized
Ovum rate 100% is adopted in artificial propagation, and rate of fertilization 90% sends out eye rate 93%, hatching rate 85%, and abnormal rate 0.5 ‰ makes variegated Du
Father's fish biological control is possibly realized, and new rare cultured fishes is provided for cold water fish industry, and then push cold water fish industry
Health efficiently develops.
Variegated sculpins artificial fecundation method, it is characterized in that: include the following steps, and following steps sequentially carry out,
Step 1: placing side by side major trough using glass reinforced plastic indoors, outlet pipe is closed, it is 20cm that the depth of water is added water into slot, is adopted
It is sterilized 24 hours with 10% povidone iodine 20ppm, repetition is changed water and scrubbed 2 times, sets at the outlet pipe front end 20cm for placing side by side slot
It is equipped with the block of 8 mesh, water filling in Xiang Pinglie slot, the depth of water is 12cm~13cm；
Step 2: on annual April 15~25, takes Changbaishan area variegated sculpins parent population of wild 3 age, by female parent population
It separately puts into place side by side in slot major trough with male parent population and temporarily support, temporarily feeding density is 10kg/m2, placing side by side slot water inlet pipe flow is 4L/min
~5L/min temporarily supports first 3 days started, when 5:00 every morning~6:00 when, slot water will be placed side by side and drain into 4cm, 2% brine concentration
After sterilizing 40min, lift water level, bait basket is set in water inlet pipe lower part, feeds chilled artemia；
Step 3: checked primary female parent population maturity every three days to 27 days, adult female parent population is picked up into 2 tails,
It is protected from light, under waterproof condition, by ovum artificial collection method, it is 13cm that ovum, which is adopted into back cut diameter, and depth is adopted in ovum basin for 6cm's；
Step 4: male parent population is picked up 1 tail, it is being protected from light, under waterproof condition, using isotonic solution semen collection, sperm is being poured
In semen collection basin, obtains and carry smart isotonic solution；
Step 5: the smart isotonic solution of load that step 4 obtains is poured into the adopting in ovum basin of step 3,5S~10S is sufficiently stirred,
Stand 3min~5min；
Step 6: the fertilized eggs that step 5 obtains are placed side by side slot hatching using the glass reinforced plastic after disinfection cleaning, 5 are set in slot
Concatenated sulculus, and the eliminator plate of each sulculus and side are sealed with the area spongiosa of long 80cm, width 5cm, thickness 2cm；Fertilized eggs patch
The water surface is uniformly splashed to placing side by side in slot, and the Sheng ovum density for placing side by side each sulculus of slot is 20000/m2~25000/m2, will contain
The slot of placing side by side of fertilized eggs is covered with buckle, be protected from light, it is shockproof under the conditions of hatched, hatching water temperature be 7.8 DEG C~10.1 DEG C, incubate
Change Dissolved Oxygen in Water >=6.5mg/L；
Step 7: the sealing area spongiosa of sulculus is removed when fertilized eggs reach emergence 3 ‰, rinse sulculus with clear water, it will be young
Fish and dead ovum pour into basin, and sulculus is moved into placing side by side in slot major trough after sterilizing and continues to hatch, and the dead egg white in basin are washed dry
Only, prelarva is poured into place side by side in slot major trough and is temporarily supported, placed side by side 30 mesh anti-escape networks of setting at slot pitch outlet pipe 20cm, place side by side the depth of water of slot
For 10cm~12cm, water flow 2.5L/min~3L/min；
Fertilized eggs are emerged at 1%, when 16:00~17:00 in afternoon, close outlet pipe, are made to hatch water level and are reached 20cm, are closed
Water inlet pipe and outlet pipe water flow are closed, the dissolved oxygen of monitoring hatching water body guarantees hatching Dissolved Oxygen in Water >=6.5mg/L, until dissolution
Oxygen rinses sulculus, prelarva and dead ovum is poured into basin, dead ovum is removed in 6.5mg/L~7mg/L, will be in prelarva investment temporarily feeding slot
Temporarily support；Water inlet pipe and outlet pipe water flow are opened, after adding water 6 hours, continues to close water flow；
Step 8: the operation of step 7 is repeated, until emergence terminates；
So far, the artificial propagation of variegated sculpins is completed.
In the step 2, parent population investment is placed side by side in slot major trough temporarily support after, when daily 5:00~6:00 when between clean with water it is flat
Column slot, the method for cleaning with water are to pull out to place side by side slot outlet pipe, are dragged from top to bottom with the fishing sea of 30 mesh, flow through residual bait, excrement with water
Block outflow.
The size for placing side by side slot is 2.8m × 0.52m × 0.22m, wherein the size of 5 series connection sulculuses is equal in step 5
For 0.4m × 0.5m × 0.2m.
In the step 4 and step 5,5 times~7 times that smart isotonic solution is female parent population egg volume are carried.
In the step 6, fertilized eggs investment is placed side by side after slot to before emerging, and is sterilized daily with bronopol solution flowing water
Once, concrete operation method is that the bronopol solution of 180g/L is taken 300mL, is fitted into titration bottle, passes through hanging bottle type
Dropper instills at the water inlet for placing side by side slot, and flowing water sterilizes fertilized eggs, places side by side the disinfection concentration 0.35g/L of bronopol solution in slot
~0.4g/L titrates disinfecting time 40min~60min.
In the step 6, hatch outlet pipe setting 11~12cm of height that slot is placed side by side used in fertilized eggs, water inlet pipe flow
Control is in 0.8L/min~1.2L/min.
Through the above design, the present invention can be brought the following benefits: variegated sculpins artificial fecundation method, real
The artificial propagation for having showed sculpins, adopts ovum rate 100%, and rate of fertilization 90% sends out eye rate 93%, hatching rate 85%, abnormal rate
0.5 ‰, make it possible variegated sculpins propagation in scale, new rare cultured fishes is provided for cold water fish industry, in turn
It pushes cold water fish industry health, efficiently develop.
Specific embodiment
Variegated sculpins artificial fecundation method, including being wanted to environmental condition, condition of water quality and to the operation of reproduction technique
It asks,
In artificial propagation first half of the month, Changbaishan area is generally 15~25 of mid or late April, will be close to sexually matured
Variegated sculpins moves into indoor glass steel and places side by side in slot major trough, specification 2.8m × 0.52m × 0.22m of major trough.Before immigration, first will
It places side by side slot water outlet bottom of the tube to be closed with plastic cloth, opens water inlet pipe, add water to 20cm, sterilized with 10% povidone iodine 20ppm
24 hours, slot was placed side by side in scrub, then pulls out outlet pipe, re-closed to place side by side slot outlet pipe after the water containing medical fluid is exhausted, and opened water inlet
Pipe, topped up with water are scrubbed 2 times.Later, the outlet pipe of 12~13cm is plugged, and the block of 8 mesh is set at 20cm before outlet pipe,
Add water, the depth of water is maintained at 12~13cm, selects the parent population in 3 ages, and female, male separate temporarily is supported, temporarily feeding density 10kg/m2, water inlet pipe stream
Amount control is in 4~5L/min；And raun is divided into according to the mature condition of raun by different batches.
Bait selects chilled artemia when temporarily supporting, and in the case where placing side by side front of the slot, water inlet pipe, bait basket is arranged, frame on placing side by side slot,
Chilled artemia is put into wherein, suitable chilled artemia is fed according to the situation of ingesting of fish, is advisable with second day slightly surplus bait；
When 5:00 every morning~6:00 when, clean with water and place side by side slot, method is: pulling out outlet pipe, with the fishing seas of 30 mesh from upper
And lower dragging, so that residual bait, excrement is flowed through block outflow with water, keeps the sanitation and hygiene for placing side by side slot breeding water body.
From April 27, a raun, mature condition of the Breeding climax according to parent population, every two days or the same day are checked every three days
It checks raun, guarantees that mature good parent population adopts ovum in time, to improve the rate of fertilization of artificial propagation.
It is being protected from light, carrying out adopting ovum, semen collection under waterproof condition, the female, male of artificial propagation compares 2:1.
Fertilized eggs place side by side slot hatching using glass reinforced plastic, and specification is 2.8m × 0.52m × 0.22m, inside set 0.4m × 0.5m
5 concatenated sulculuses of × 0.2m.Before incubating oosperm, slot water outlet bottom of the tube will be placed side by side and closed with plastic cloth, 20cm is added water to,
It is sterilized 24 hours with the povidone iodine 20ppm of 10% content, then, scrubs sulculus and major trough, outlet pipe is pulled out, by the water of disinfection
Discharge.Outlet pipe, topped up with water are closed, then cleans sulculus and major trough twice, glass reinforced plastic is placed side by side into slot strict sterilization.To use hatching
Water flows through each sulculus by top-down, and the eliminator plate of every sulculus and side are close with long 80cm, width 5cm, the area spongiosa of thickness 2cm
Envelope.Outlet pipe setting 11~12cm of height, water inlet pipe flow control are 8~10.5 DEG C of water source water temperature, molten in 0.8~1.2L/min
Solve 9~11mg/L of oxygen.
Crowded abdomen adopts ovum hair and adopts ovum.It is up to mature raun and picks up 2 tails, the water of body surface is wiped with dry cotton towel, adopts ovum
People wears medical rubber glove, holds caudal peduncle on the other hand, on the other hand soft abdomen, and the ovum of mature variegated sculpins is adopted into back cut diameter
The drying of 13cm, depth 6cm are adopted in ovum basin.
It, should semen collection immediately after ovum is adopted.Since the semen volume of variegated sculpins milter is small, its mature feelings cannot be checked in advance
Condition can not directly adopt sperm in ovum basin, so, using isotonic solution semen collection.Method is: first containing isotonic solution into plastics
In beaker, a tail milter is picked up, the water of body surface is wiped with dry cotton towel, the light cloaca for pressing milter has held Sheng until sperm flows out
The plastic beaker of isotonic solution is poured the sperm of milter in dry semen collection basin with isotonic solution.If milter is without maturation, Ying Li
It fishes for again, repeats above step.It is sufficiently stirred for the ease of smart, ovum, according to the volume situation of ovum, carries the isotonic of essence
Liquid should be the volume of ovum 5~7 times are advisable.
Insemination method is that isotonic solution carries essence insemination, i.e., pours into the isotonic solution for being loaded with sperm and adopt in ovum basin, filled immediately with hand
Divide stirring 5~10s, stands 3~5min.
Contain ovum first from the sulculus of water outlet Sheng, contain ovum people end Sheng ovum basin, uniformly splash close to the water surface, avoid placing side by side
Water in slot enters in basin, causes mutually being adhered for fertilized eggs, place side by side the Sheng ovum density 20000~25000 of the every sulculus of slot/
m2。
The glass reinforced plastic for having contained fertilized eggs is placed side by side slot to be covered with buckle, be protected from light, it is shockproof under the conditions of hatched.
Dissolved Oxygen in Water >=6.5mg/L is hatched in 7.8~10.1 DEG C of water temperature of hatching.
Fertilized eggs use the disinfection of bronopol solution flowing water primary daily.Method is: the bronopol of 180g/L is molten
Liquid takes 300mL, is fitted into titration bottle, is instilled at the water inlet for placing side by side slot by the dropper of hanging bottle type, and flowing water sterilizes fertilized eggs, places side by side
0.35~0.4g/L of disinfection concentration of bronopol solution in slot titrates 40~60min of disinfecting time, until before emergence.
Fertilized eggs are emerged at 3 ‰, the sealing area spongiosa of sulculus are removed, while gently rinsing sulculus with clear water, will be young
Fish, dead ovum pour into basin, then, sulculus is moved into disinfect place side by side in slot major trough and continue to hatch, the dead egg white in basin is washed
Completely, prelarva pour into away from be arranged at outlet pipe 20cm 30 mesh anti-escape networks disinfect place side by side in slot and temporarily support, trough inner water deep 10
~12cm, 2.5~3L/min of water flow.
Eyed eggs choose dead ovum during emergence as much as possible daily.When emerging 1%, afternoon 16:00~17:
When 00, outlet pipe is closed, hatching water level is made to reach 20cm, closes water flow, the dissolved oxygen of monitoring hatching water body guarantees to hatch water body
Dissolved oxygen >=6.5mg/L pours into prelarva and dead ovum in basin until dissolved oxygen is in 6.5~7mg/L, then gently rinses sulculus, rinses
Completely, prelarva is put into temporarily feeding slot.Then, outlet pipe is opened, after adding water 6 hours, closing water outlet bottom of the tube continues to close water
Stream so repeats, until emergence terminates.
The variegated sculpins hatching rate bred by the method for the invention can reach 85% or more, and hatching prelarva reaches 13.8 ten thousand
Tail provides new rare cultured fishes for cold water fish industry.
Claims (6)
1. variegated sculpins artificial fecundation method, it is characterized in that: include the following steps, and following steps sequentially carry out,
Step 1: placing side by side slot major trough using glass reinforced plastic indoors, outlet pipe is closed, it is 20cm that the depth of water is added water into slot, is used
10% povidone iodine 20ppm is sterilized 24 hours, and repetition is changed water and scrubbed 2 times, is arranged at the outlet pipe front end 20cm for placing side by side slot
There are the block of 8 mesh, water filling in Xiang Pinglie slot, the depth of water is 12cm~13cm；
Step 2: on annual April 15~25, takes Changbaishan area variegated sculpins parent population of wild 3 age, by female parent population and hero
Property parent population separately put into place side by side and temporarily supported in slot major trough, temporarily feeding density is 10kg/m2, place side by side slot water inlet pipe flow be 4L/min~
5L/min temporarily supports first 3 days started, when 5:00 every morning~6:00 when, slot water will be placed side by side and drain into 4cm, 2% brine concentration disappears
After malicious 40min, lift water level, bait basket is set in water inlet pipe lower part, feeds chilled artemia；
Step 3: checking primary female parent population maturity every three days to 27 days, adult female parent population being picked up 2 tails, is being kept away
Under light, waterproof condition, by ovum artificial collection method, it is 13cm that ovum, which is adopted into back cut diameter, and depth is adopted in ovum basin for 6cm's；
Step 4: male parent population is picked up 1 tail, it is being protected from light, under waterproof condition, using isotonic solution semen collection, sperm is poured into semen collection
In basin, obtains and carry smart isotonic solution；
Step 5: the smart isotonic solution of load that step 4 obtains is poured into the adopting in ovum basin of step 3,5S~10S is sufficiently stirred, stands
3min~5min；
Step 6: the fertilized eggs that step 5 obtains are placed side by side slot hatching using the glass reinforced plastic after disinfection cleaning, 5 series connection are set in slot
Sulculus, and the eliminator plate of each sulculus and side are sealed with the area spongiosa of long 80cm, width 5cm, thickness 2cm；Fertilized eggs agio face
It uniformly splashes to placing side by side in slot, the Sheng ovum density for placing side by side each sulculus of slot is 20000/m2~25000/m2, Sheng is felt better essence
The slot of placing side by side of ovum is covered with buckle, be protected from light, it is shockproof under the conditions of hatched, hatching water temperature be 7.8 DEG C~10.1 DEG C, hatch water
Body dissolved oxygen >=6.5mg/L；
Step 7: removing the sealing area spongiosa of sulculus when fertilized eggs reach emergence 3 ‰, rinse sulculus with clear water, by prelarva and
Dead ovum pours into basin, and sulculus is moved into placing side by side in slot major trough after sterilizing and continues to hatch, will by the dead egg white wash clean in basin
Prelarva pours into place side by side and temporarily support in slot major trough, places side by side 30 mesh anti-escape networks of setting, the depth of water for placing side by side slot at slot pitch outlet pipe 20cm and is
10cm~12cm, water flow 2.5L/min~3L/min；
Fertilized eggs are emerged at 1%, when 16:00~17:00 in afternoon, close outlet pipe, are made to hatch water level and are reached 20cm, and are closed
Water inlet pipe and outlet pipe water flow, the dissolved oxygen of monitoring hatching water body, guarantee hatching Dissolved Oxygen in Water >=6.5mg/L, until dissolved oxygen
In 6.5mg/L~7mg/L, sulculus is rinsed, prelarva and dead ovum are poured into basin, dead ovum is removed, it will be temporary in prelarva investment temporarily feeding slot
It supports；Water inlet pipe and outlet pipe water flow are opened, after adding water 6 hours, continues to close water flow；
Step 8: the operation of step 7 is repeated, until emergence terminates；
So far, the artificial propagation of variegated sculpins is completed.
2. variegated sculpins artificial fecundation method according to claim 1, it is characterized in that: parent population is thrown in the step 2
Enter to place side by side in slot major trough after temporarily supporting, when daily 5:00~6:00 when between clean with water and place side by side slot, the method for cleaning with water is to pull out to place side by side slot and go out
Water pipe is dragged from top to bottom with the fishing sea of 30 mesh, and residual bait, excrement is made to flow through block outflow with water.
3. variegated sculpins artificial fecundation method according to claim 1, it is characterized in that: the size for placing side by side slot is
2.8m × 0.52m × 0.22m, wherein the size of 5 series connection sulculuses is 0.4m × 0.5m × 0.2m in step 5.
4. variegated sculpins artificial fecundation method according to claim 1, it is characterized in that: the step 4 and step 5
In, carry 5 times~7 times that smart isotonic solution is female parent population egg volume.
5. variegated sculpins artificial fecundation method according to claim 1, it is characterized in that: in the step 6, fertilized eggs
It puts into after placing side by side slot to before emerging, uses bronopol solution flowing water to sterilize daily primary, concrete operation method is, by 180g/L
Bronopol solution take 300mL, be fitted into titration bottle, instilled at the water inlet for placing side by side slot by the dropper of hanging bottle type, flowing water
Fertilized eggs are sterilized, disinfection concentration 0.35g/L~0.4g/L of bronopol solution in slot is placed side by side, titrate disinfecting time 40min
~60min.
6. variegated sculpins artificial fecundation method according to claim 1, it is characterized in that: in the step 6, hatching by
Outlet pipe setting 11~12cm of height of slot is placed side by side used in smart ovum, water inlet pipe flow control is in 0.8L/min~1.2L/min.
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| CN201910823114.9A CN110367157B (en) | 2019-09-02 | 2019-09-02 | Artificial breeding method for variegated father-fish |
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| CN201910823114.9A CN110367157B (en) | 2019-09-02 | 2019-09-02 | Artificial breeding method for variegated father-fish |
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| CN110367157B CN110367157B (en) | 2021-08-06 |
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