CN103430892B - Production method of germ cell of pearl giant grouper - Google Patents
Production method of germ cell of pearl giant grouper Download PDFInfo
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- CN103430892B CN103430892B CN201310400307.6A CN201310400307A CN103430892B CN 103430892 B CN103430892 B CN 103430892B CN 201310400307 A CN201310400307 A CN 201310400307A CN 103430892 B CN103430892 B CN 103430892B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to a kind of production method of germ cell of pearl giant grouper.Breeding method of the present invention is: 1. seedling raising environment condition, 2. parent population is selected, 3. to hasten parturition fertilization preparation, 4. to hasten parturition fertilization, 5. hatch, the invention has the beneficial effects as follows: have employed production method of germ cell of pearl giant grouper of the present invention to have good operation, fertilization rate is high and be subject to the advantages such as extraneous effect of natural conditions is little.
Description
Technical field
The present invention relates to a kind of production method of germ cell of pearl giant grouper.
Background technology
Pearl rough gentian grouper is the most popular mariculture kind in current Hainan, is got by the sperm of rough gentian grouper and the ovum fertilization of epinephelus fuscoguttatus.In recent years, Grouper cultivating scale obtains and develops rapidly, and Product quality and safety receives much attention.Pearl rough gentian seed demand is huge, but the precondition meeting Seedling production is the production of fertilized egg.Although the research of current China to grouper hybridization technique has certain breakthrough, also there is many problems; Success of the present invention, solves a kind of production method of germ cell of pearl giant grouper, effectively can improve the output of grouper fertilized egg, meet the demand in market; It is that the world initiates that this fertilized egg produces technology.
Summary of the invention
The object of the present invention is to provide a kind of saddle production method of germ cell of pearl giant grouper.
1. production method of germ cell of pearl giant grouper, is characterized in that:
One, environmental condition
Pond cultivated by a, cement
Area 15 ~ 35m
2rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.0 ~ 1.2m in pond;
B, fertilization and hatching bucket
The black glass steel drum of diameter 0.8 ~ 1.2m, high 1 ~ 1.2m;
C, fertilization basin
Diameter 0.6 ~ 0.8m, the plastic basin of high 20 ~ 30cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.0 ~ 8.5, salinity 30 ~ 40 ‰, water temperature 26 ~ 33 DEG C, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, and without difference, disease-free healthy mature is individual, with hand toward belly firmly extruding, can discharge seminal fluid smoothly;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, and without difference, disease-free healthy mature is individual, and belly is swell very, and soft cotton;
Three, to hasten parturition fertilization preparation
A, clean
Wash, sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, consumption usage performs according to the regulation of NY 5071; Rinse after immersion and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 80 ~ 100cm the previous day, 400 mesh sieve tulles filter, and inflate in a large number, arrange the oxygenation of 1 gas stone for every square metre in pond;
C, hatching pail inject new water 80 ~ 90cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and inflate in a large number;
Four, to hasten parturition fertilization
A, inject the LHRH-A2 of 30 ~ 700,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, when closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 36 ~ 42 hours, can artificial induced spawning be carried out, parent population is placed in suitable fishing net, firmly extrude belly, release ovum and put in fertilization basin; Add 100ml seminal fluid to be immediately fertilized, then add the clean seawater of 3 ~ 6L, and ceaselessly stir companion 2 ~ 5min with hand;
The seawater that the fertilized egg of d, after fertilization is clean cleans 2 ~ 5 times;
Five, hatch
A, hatching density
Hatching density is: 0.6 ~ 1kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum process
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 20 ~ 30min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 20 ~ 30min carries out for the first time, within 1 ~ 3 hour backward, carry out once;
C, change water
After processing bad ovum each time, the remaining fertilized egg bubbled through the water column in upper or water picks up with fishing net, puts into another hatching pail; Carried out once every 1 ~ 3 hour, until hatch prelarva.
The invention has the beneficial effects as follows: have employed production method of germ cell of pearl giant grouper of the present invention to have good operation, fertilization rate is high and be subject to the advantages such as extraneous effect of natural conditions is little.
Embodiment
Embodiment 1:
Production method of germ cell of pearl giant grouper:
One, environmental condition
Pond cultivated by a, cement
Area 15m
2rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.0m in pond;
B, fertilization and hatching bucket
The black glass steel drum of diameter 0.8m, high 1m;
C, fertilization basin
Diameter 0.6m, the plastic basin of high 20cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.0, salinity 30 ‰, water temperature 26 DEG C, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, and without difference, disease-free healthy mature is individual, with hand toward belly firmly extruding, can discharge seminal fluid smoothly;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, and without difference, disease-free healthy mature is individual, and belly is swell very, and soft cotton;
Three, to hasten parturition fertilization preparation
A, clean
Wash, sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, consumption usage performs according to the regulation of NY 5071; Rinse after immersion and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 80cm the previous day, 400 mesh sieve tulles filter, and inflate in a large number, arrange the oxygenation of 1 gas stone for every square metre in pond;
C, hatching pail inject new water 80cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and inflate in a large number;
Four, to hasten parturition fertilization
A, inject the LHRH-A2 of 300,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, when closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 36 hours, can artificial induced spawning be carried out, parent population is placed in suitable fishing net, firmly extrude belly, release ovum and put in fertilization basin; Add 100ml seminal fluid to be immediately fertilized, then add the clean seawater of 3L, and ceaselessly stir companion 2min with hand;
The seawater that the fertilized egg of d, after fertilization is clean cleans 2 times;
Five, hatch
A, hatching density
Hatching density is: 0.6kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum process
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 20min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 20min carries out for the first time, within 1 hour backward, carry out once;
C, change water
After processing bad ovum each time, the remaining fertilized egg bubbled through the water column in upper or water picks up with fishing net, puts into another hatching pail; Carried out once every 1 hour, until hatch prelarva.
Embodiment 2:
Production method of germ cell of pearl giant grouper, is characterized in that:
One, environmental condition
Pond cultivated by a, cement
Area 28m
2rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.1m in pond;
B, fertilization and hatching bucket
The black glass steel drum of diameter 1.0m, high 1.1m;
C, fertilization basin
Diameter 0.7m, the plastic basin of high 25cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.3, salinity 35 ‰, water temperature 29 DEG C, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, and without difference, disease-free healthy mature is individual, with hand toward belly firmly extruding, can discharge seminal fluid smoothly;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, and without difference, disease-free healthy mature is individual, and belly is swell very, and soft cotton;
Three, to hasten parturition fertilization preparation
A, clean
Wash, sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, consumption usage performs according to the regulation of NY 5071; Rinse after immersion and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 90cm the previous day, 400 mesh sieve tulles filter, and inflate in a large number, arrange the oxygenation of 1 gas stone for every square metre in pond;
C, hatching pail inject new water 85cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and inflate in a large number;
Four, to hasten parturition fertilization
A, inject the LHRH-A2 of 500,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, when closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 39 hours, can artificial induced spawning be carried out, parent population is placed in suitable fishing net, firmly extrude belly, release ovum and put in fertilization basin; Add 100ml seminal fluid to be immediately fertilized, then add the clean seawater of 4.5L, and ceaselessly stir companion 3.5min with hand;
The seawater that the fertilized egg of d, after fertilization is clean cleans 3.5 times;
Five, hatch
A, hatching density
Hatching density is: 0.8kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum process
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 25min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 25min carries out for the first time, within 2 hours backward, carry out once;
C, change water
After processing bad ovum each time, the remaining fertilized egg bubbled through the water column in upper or water picks up with fishing net, puts into another hatching pail; Carried out once every 2 hours, until hatch prelarva.
Embodiment 3:
Production method of germ cell of pearl giant grouper:
One, environmental condition
Pond cultivated by a, cement
Area 35m
2rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.2m in pond;
B, fertilization and hatching bucket
The black glass steel drum of diameter 1.2m, high 1.2m;
C, fertilization basin
Diameter 0.8m, the plastic basin of high 30cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.5, salinity 40 ‰, water temperature 33 DEG C, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, and without difference, disease-free healthy mature is individual, with hand toward belly firmly extruding, can discharge seminal fluid smoothly;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, and without difference, disease-free healthy mature is individual, and belly is swell very, and soft cotton;
Three, to hasten parturition fertilization preparation
A, clean
Wash, sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, consumption usage performs according to the regulation of NY 5071; Rinse after immersion and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 100cm the previous day, 400 mesh sieve tulles filter, and inflate in a large number, arrange the oxygenation of 1 gas stone for every square metre in pond;
C, hatching pail inject new water 90cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and inflate in a large number;
Four, to hasten parturition fertilization
A, inject the LHRH-A2 of 700,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, when closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 42 hours, can artificial induced spawning be carried out, parent population is placed in suitable fishing net, firmly extrude belly, release ovum and put in fertilization basin; Add 100ml seminal fluid to be immediately fertilized, then add the clean seawater of 6L, and ceaselessly stir companion 5min with hand;
The seawater that the fertilized egg of d, after fertilization is clean cleans 5 times;
Five, hatch
A, hatching density
Hatching density is: 1kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum process
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 30min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 30min carries out for the first time, within 3 hours backward, carry out once;
C, change water
After processing bad ovum each time, the remaining fertilized egg bubbled through the water column in upper or water picks up with fishing net, puts into another hatching pail; Carried out once every 3 hours, until hatch prelarva.
Claims (1)
1. production method of germ cell of pearl giant grouper, is characterized in that:
One, environmental condition
Pond cultivated by a, cement
Area 15 ~ 35m
2rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.0 ~ 1.2m in pond;
B, hatching pail
The black glass steel drum of diameter 0.8 ~ 1.2m, high 1 ~ 1.2m;
C, fertilization basin
Diameter 0.6 ~ 0.8m, the plastic basin of high 20 ~ 30cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.0 ~ 8.5, salinity 30 ~ 40 ‰, water temperature 26 ~ 33 DEG C, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, and without difference, disease-free healthy mature is individual, with hand toward belly firmly extruding, can discharge seminal fluid smoothly;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, and without difference, disease-free healthy mature is individual, and belly is swell very, and soft;
Three, to hasten parturition fertilization preparation
A, clean
Wash, sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, consumption usage performs according to the regulation of NY 5071; Rinse after immersion and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 80 ~ 100cm the previous day, 400 mesh sieve tulles filter, and inflate in a large number, arrange the oxygenation of 1 gas stone for every square metre in pond;
C, hatching pail inject new water 80 ~ 90cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and inflate in a large number;
Four, to hasten parturition fertilization
A, inject the LHRH-A2 of 30 ~ 700,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, when closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 36 ~ 42 hours, can artificial induced spawning be carried out, parent population is placed in suitable fishing net, firmly extrude belly, release ovum and put in fertilization basin; Add 100ml seminal fluid to be immediately fertilized, then add the clean seawater of 3 ~ 6L, and ceaselessly stir 2 ~ 5min with hand;
The seawater that the fertilized egg of d, after fertilization is clean cleans 2 ~ 5 times;
Five, hatch
A, hatching density
Hatching density is: 0.6 ~ 1kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum process
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 20 ~ 30min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 20 ~ 30min carries out for the first time, within 1 ~ 3 hour backward, carry out once;
C, change water
After processing bad ovum each time, the remaining fertilized egg bubbled through the water column in upper or water picks up with fishing net, puts into another hatching pail; Carried out once every 1 ~ 3 hour, until hatch prelarva.
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| CN201310400307.6A CN103430892B (en) | 2013-09-06 | 2013-09-06 | Production method of germ cell of pearl giant grouper |
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| CN201310400307.6A CN103430892B (en) | 2013-09-06 | 2013-09-06 | Production method of germ cell of pearl giant grouper |
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| CN103430892A CN103430892A (en) | 2013-12-11 |
| CN103430892B true CN103430892B (en) | 2015-11-04 |
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| CN107568116B (en) * | 2017-10-17 | 2019-01-18 | 海南晨海水产有限公司 | A kind of artificial fecundation method of China fir spot grouper |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700001A (en) * | 2009-11-19 | 2010-05-05 | 海南定大养殖有限公司 | Industrial artificial breeding method of epinephelus lanceolatus |
| CN101933476A (en) * | 2010-08-09 | 2011-01-05 | 广东省大亚湾水产试验中心 | Method for breeding cross-bred 'Hulongban' fish |
| CN103026994A (en) * | 2013-01-04 | 2013-04-10 | 莱州明波水产有限公司 | Factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus |
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2013
- 2013-09-06 CN CN201310400307.6A patent/CN103430892B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700001A (en) * | 2009-11-19 | 2010-05-05 | 海南定大养殖有限公司 | Industrial artificial breeding method of epinephelus lanceolatus |
| CN101933476A (en) * | 2010-08-09 | 2011-01-05 | 广东省大亚湾水产试验中心 | Method for breeding cross-bred 'Hulongban' fish |
| CN103026994A (en) * | 2013-01-04 | 2013-04-10 | 莱州明波水产有限公司 | Factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus |
Non-Patent Citations (2)
| Title |
|---|
| "点带石斑鱼亲鱼培育、产卵和孵化的试验研究";陈国华等;《海洋与湖沼》;20010731;第32卷(第4期);第428-435页 * |
| 石斑鱼人工繁育技术研究进展;何永亮等;《南方水产》;20080630;第4卷(第3期);第75-79页 * |
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Effective date of registration: 20210527 Address after: 570125 room 1401, 6 Jinhai Plaza, Jinlong Road, Longhua District, Haikou, Hainan. Patentee after: Hainan blue Thai Bio Technology Co.,Ltd. Address before: 571343 No.23, Huaqiao new village, Huiwen Town, Wenchang City, Hainan Province Patentee before: HAINAN DINGDA CULTIVATION Co.,Ltd. |