CN103430892A - Production method of germ cell of pearl giant grouper - Google Patents
Production method of germ cell of pearl giant grouper Download PDFInfo
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- CN103430892A CN103430892A CN2013104003076A CN201310400307A CN103430892A CN 103430892 A CN103430892 A CN 103430892A CN 2013104003076 A CN2013104003076 A CN 2013104003076A CN 201310400307 A CN201310400307 A CN 201310400307A CN 103430892 A CN103430892 A CN 103430892A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a production method of a germ cell of pearl giant grouper. The cultivating method disclosed by the invention comprises: 1, determining of a feeding environment condition; 2, parent fish selection; 3, oxytocin fertilization preparation work; 4, oxytocin fertilization; 5, incubation. The production method has the beneficial effects that after the production method of the germ cell of the pearl giant grouper disclosed by the invention is adopted, the advantages of operation is easy, fertilization rate is high, influence of an external natural condition is low, and the like are achieved.
Description
Technical field
The present invention relates to a kind of pearl rough gentian grouper fertilized egg production method.
Background technology
Pearl rough gentian grouper is the most popular mariculture kind in current Hainan, is to be got by the sperm of rough gentian grouper and the ovum fertilization of epinephelus fuscoguttatus.In recent years, the Grouper cultivating scale has obtained development rapidly, and Product quality and safety receives much attention.Pearl rough gentian seed demand is huge, but meet the precondition that seed produces, is the production of fertilized egg.Although there is certain breakthrough in China to the research of grouper hybridization technique at present, also exists many problems; Success of the present invention, solved a kind of pearl rough gentian grouper fertilized egg production method, can effectively improve the output of grouper fertilized egg, the demand of satisfying the market; This fertilized egg generating technique is that the world is pioneering.
Summary of the invention
The object of the present invention is to provide a kind of saddle pearl rough gentian grouper fertilized egg production method.
1. pearl rough gentian grouper fertilized egg production method is characterized in that:
One, environmental condition
A, cement are cultivated pond
Area 15～35m
2the rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.0～1.2m in pond;
B, fertilization and hatching bucket
Diameter 0.8～1.2m, the black glass steel drum of high 1～1.2m;
C, fertilization basin
Diameter 0.6～0.8m, the plastic basin of high 20～30cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.0～8.5, salinity 30～40 ‰, 26～33 ℃ of water temperatures, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, nothing difference, disease-free healthy mature individuality, firmly push toward belly with hand, can discharge smoothly seminal fluid;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, nothing difference, disease-free healthy mature individuality, belly is swell very, and soft cotton;
Three, the fertilization preparation of hastening parturition
A, clean
Wash, the sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, and the consumption usage is carried out according to the regulation of NY 5071; Soak later and rinse and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 80～100cm the previous day, 400 mesh sieve tulles filter, and a large amount of inflation, in pond, arrange the oxygenation of 1 gas stone for every square metre;
C, hatching pail inject new water 80～90cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and a large amount of inflation;
Four, the fertilization of hastening parturition
A, inject the LHRH-A2 of 30～700,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, while closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 36～42 hours, can carry out artificial induced spawning, parent population is placed in suitable fishing net, firmly push belly, release ovum and put in the fertilization basin; Add immediately the 100ml seminal fluid to be fertilized, then add the clean seawater of 3～6L, and ceaselessly stir companion 2～5min with hand;
The fertilized egg of d, after fertilization is cleaned 2～5 times with clean seawater;
Five, hatching
A, hatching density
Hatching density is: 0.6～1kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum are processed
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 20～30min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 20～30min carries out for the first time, within 1～3 hour backward, carry out once;
C, change water
After processing bad ovum each time, the fertilized egg in remaining bubble through the water column upper or water picks up with fishing net, puts into another hatching pail; Every 1～3 hour, carry out once, until hatch prelarva.
The invention has the beneficial effects as follows: adopted pearl rough gentian grouper fertilized egg production method of the present invention, had good operation, fertilization rate is high and be subject to the advantages such as extraneous effect of natural conditions is little.
Embodiment
Embodiment 1:
Pearl rough gentian grouper fertilized egg production method:
One, environmental condition
A, cement are cultivated pond
Area 15m
2the rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.0m in pond;
B, fertilization and hatching bucket
Diameter 0.8m, the black glass steel drum of high 1m;
C, fertilization basin
Diameter 0.6m, the plastic basin of high 20cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.0, salinity 30 ‰, 26 ℃ of water temperatures, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, nothing difference, disease-free healthy mature individuality, firmly push toward belly with hand, can discharge smoothly seminal fluid;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, nothing difference, disease-free healthy mature individuality, belly is swell very, and soft cotton;
Three, the fertilization preparation of hastening parturition
A, clean
Wash, the sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, and the consumption usage is carried out according to the regulation of NY 5071; Soak later and rinse and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 80cm the previous day, 400 mesh sieve tulles filter, and a large amount of inflation, in pond, arrange the oxygenation of 1 gas stone for every square metre;
C, hatching pail inject new water 80cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and a large amount of inflation;
Four, the fertilization of hastening parturition
A, inject the LHRH-A2 of 300,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, while closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 36 hours, can carry out artificial induced spawning, parent population is placed in suitable fishing net, firmly push belly, release ovum and put in the fertilization basin; Add immediately the 100ml seminal fluid to be fertilized, then add the clean seawater of 3L, and ceaselessly stir companion 2min with hand;
The fertilized egg of d, after fertilization is cleaned 2 times with clean seawater;
Five, hatching
A, hatching density
Hatching density is: 0.6kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum are processed
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 20min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 20min carries out for the first time, within 1 hour backward, carry out once;
C, change water
After processing bad ovum each time, the fertilized egg in remaining bubble through the water column upper or water picks up with fishing net, puts into another hatching pail; Every 1 hour, carry out once, until hatch prelarva.
Embodiment 2:
Pearl rough gentian grouper fertilized egg production method is characterized in that:
One, environmental condition
A, cement are cultivated pond
Area 28m
2the rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.1m in pond;
B, fertilization and hatching bucket
Diameter 1.0m, the black glass steel drum of high 1.1m;
C, fertilization basin
Diameter 0.7m, the plastic basin of high 25cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.3, salinity 35 ‰, 29 ℃ of water temperatures, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, nothing difference, disease-free healthy mature individuality, firmly push toward belly with hand, can discharge smoothly seminal fluid;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, nothing difference, disease-free healthy mature individuality, belly is swell very, and soft cotton;
Three, the fertilization preparation of hastening parturition
A, clean
Wash, the sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, and the consumption usage is carried out according to the regulation of NY 5071; Soak later and rinse and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 90cm the previous day, 400 mesh sieve tulles filter, and a large amount of inflation, in pond, arrange the oxygenation of 1 gas stone for every square metre;
C, hatching pail inject new water 85cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and a large amount of inflation;
Four, the fertilization of hastening parturition
A, inject the LHRH-A2 of 500,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, while closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 39 hours, can carry out artificial induced spawning, parent population is placed in suitable fishing net, firmly push belly, release ovum and put in the fertilization basin; Add immediately the 100ml seminal fluid to be fertilized, then add the clean seawater of 4.5L, and ceaselessly stir companion 3.5min with hand;
The fertilized egg of d, after fertilization is cleaned 3.5 times with clean seawater;
Five, hatching
A, hatching density
Hatching density is: 0.8kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum are processed
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 25min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 25min carries out for the first time, within 2 hours backward, carry out once;
C, change water
After processing bad ovum each time, the fertilized egg in remaining bubble through the water column upper or water picks up with fishing net, puts into another hatching pail; Every 2 hours, carry out once, until hatch prelarva.
Embodiment 3:
Pearl rough gentian grouper fertilized egg production method:
One, environmental condition
A, cement are cultivated pond
Area 35m
2the rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.2m in pond;
B, fertilization and hatching bucket
Diameter 1.2m, the black glass steel drum of high 1.2m;
C, fertilization basin
Diameter 0.8m, the plastic basin of high 30cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.5, salinity 40 ‰, 33 ℃ of water temperatures, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, nothing difference, disease-free healthy mature individuality, firmly push toward belly with hand, can discharge smoothly seminal fluid;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, nothing difference, disease-free healthy mature individuality, belly is swell very, and soft cotton;
Three, the fertilization preparation of hastening parturition
A, clean
Wash, the sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, and the consumption usage is carried out according to the regulation of NY 5071; Soak later and rinse and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 100cm the previous day, 400 mesh sieve tulles filter, and a large amount of inflation, in pond, arrange the oxygenation of 1 gas stone for every square metre;
C, hatching pail inject new water 90cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and a large amount of inflation;
Four, the fertilization of hastening parturition
A, inject the LHRH-A2 of 700,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, while closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 42 hours, can carry out artificial induced spawning, parent population is placed in suitable fishing net, firmly push belly, release ovum and put in the fertilization basin; Add immediately the 100ml seminal fluid to be fertilized, then add the clean seawater of 6L, and ceaselessly stir companion 5min with hand;
The fertilized egg of d, after fertilization is cleaned 5 times with clean seawater;
Five, hatching
A, hatching density
Hatching density is: 1kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum are processed
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 30min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 30min carries out for the first time, within 3 hours backward, carry out once;
C, change water
After processing bad ovum each time, the fertilized egg in remaining bubble through the water column upper or water picks up with fishing net, puts into another hatching pail; Every 3 hours, carry out once, until hatch prelarva.
Claims (1)
1. pearl rough gentian grouper fertilized egg production method is characterized in that:
One, environmental condition
A, cement are cultivated pond
Area 15～35m
2the rectangle cement pit, arc chord angle, at the bottom of pond, pool wall is smooth, the dark 1.0～1.2m in pond;
B, fertilization and hatching bucket
Diameter 0.8～1.2m, the black glass steel drum of high 1～1.2m;
C, fertilization basin
Diameter 0.6～0.8m, the plastic basin of high 20～30cm;
D, condition of water quality
More than dissolved oxygen 5mg/L, pH value 8.0～8.5, salinity 30～40 ‰, 26～33 ℃ of water temperatures, indoor construction controllable dimming facility, ventilation condition is good;
Two, parent population is selected
Male: epinephelus lanceolatus fish male parent, build all claims, nothing difference, disease-free healthy mature individuality, firmly push toward belly with hand, can discharge smoothly seminal fluid;
Female parent: the female parent of epinephelus fuscoguttatus, build all claims, nothing difference, disease-free healthy mature individuality, belly is swell very, and soft cotton;
Three, the fertilization preparation of hastening parturition
A, clean
Wash, the sterilization nursery pond, be watered full pool spilling head with strong chlorine oil or potassium permanganate, and the consumption usage is carried out according to the regulation of NY 5071; Soak later and rinse and drain, ventilate and shine pond;
B, water inlet
Hasten parturition and inject new water 80～100cm the previous day, 400 mesh sieve tulles filter, and a large amount of inflation, in pond, arrange the oxygenation of 1 gas stone for every square metre;
C, hatching pail inject new water 80～90cm, and 400 mesh sieve tulles filter, and put the oxygenation of 1 gas stone, and a large amount of inflation;
Four, the fertilization of hastening parturition
A, inject the LHRH-A2 of 30～700,000 unit/kg at the back of the body of epinephelus fuscoguttatus parent population;
B, while closing on artificial induced spawning, pick up male epinephelus lanceolatus fish parent fish, extrude seminal fluid, and put into refrigerator and cooled and hide;
C, after 36～42 hours, can carry out artificial induced spawning, parent population is placed in suitable fishing net, firmly push belly, release ovum and put in the fertilization basin; Add immediately the 100ml seminal fluid to be fertilized, then add the clean seawater of 3～6L, and ceaselessly stir companion 2～5min with hand;
The fertilized egg of d, after fertilization is cleaned 2～5 times with clean seawater;
Five, hatching
A, hatching density
Hatching density is: 0.6～1kg/m
3, micro-inflation incessantly in hatching pail;
B, bad ovum are processed
Fertilized egg is placed in hatching pail and stops the supple of gas or steam after 20～30min, allows bad ovum fully precipitate, then discharges bad ovum by beneath discharge outlet; Be that after dropping into fertilized egg, 20～30min carries out for the first time, within 1～3 hour backward, carry out once;
C, change water
After processing bad ovum each time, the fertilized egg in remaining bubble through the water column upper or water picks up with fishing net, puts into another hatching pail; Every 1～3 hour, carry out once, until hatch prelarva.
Priority Applications (1)
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| CN201310400307.6A CN103430892B (en) | 2013-09-06 | 2013-09-06 | Production method of germ cell of pearl giant grouper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310400307.6A CN103430892B (en) | 2013-09-06 | 2013-09-06 | Production method of germ cell of pearl giant grouper |
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| CN103430892A true CN103430892A (en) | 2013-12-11 |
| CN103430892B CN103430892B (en) | 2015-11-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568116A (en) * | 2017-10-17 | 2018-01-12 | 海南晨海水产有限公司 | A kind of artificial fecundation method of China fir spot grouper |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700001A (en) * | 2009-11-19 | 2010-05-05 | 海南定大养殖有限公司 | Industrial artificial breeding method of epinephelus lanceolatus |
| CN101933476A (en) * | 2010-08-09 | 2011-01-05 | 广东省大亚湾水产试验中心 | Method for breeding cross-bred 'Hulongban' fish |
| CN103026994A (en) * | 2013-01-04 | 2013-04-10 | 莱州明波水产有限公司 | Factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus |
-
2013
- 2013-09-06 CN CN201310400307.6A patent/CN103430892B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700001A (en) * | 2009-11-19 | 2010-05-05 | 海南定大养殖有限公司 | Industrial artificial breeding method of epinephelus lanceolatus |
| CN101933476A (en) * | 2010-08-09 | 2011-01-05 | 广东省大亚湾水产试验中心 | Method for breeding cross-bred 'Hulongban' fish |
| CN103026994A (en) * | 2013-01-04 | 2013-04-10 | 莱州明波水产有限公司 | Factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus |
Non-Patent Citations (2)
| Title |
|---|
| 何永亮等: "石斑鱼人工繁育技术研究进展", 《南方水产》 * |
| 陈国华等: ""点带石斑鱼亲鱼培育、产卵和孵化的试验研究"", 《海洋与湖沼》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107568116A (en) * | 2017-10-17 | 2018-01-12 | 海南晨海水产有限公司 | A kind of artificial fecundation method of China fir spot grouper |
| CN107568116B (en) * | 2017-10-17 | 2019-01-18 | 海南晨海水产有限公司 | A kind of artificial fecundation method of China fir spot grouper |
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Effective date of registration: 20210527 Address after: 570125 room 1401, 6 Jinhai Plaza, Jinlong Road, Longhua District, Haikou, Hainan. Patentee after: Hainan blue Thai Bio Technology Co.,Ltd. Address before: 571343 No.23, Huaqiao new village, Huiwen Town, Wenchang City, Hainan Province Patentee before: HAINAN DINGDA CULTIVATION Co.,Ltd. |