CN103392506A - Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method - Google Patents
Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method Download PDFInfo
- Publication number
- CN103392506A CN103392506A CN2013103392607A CN201310339260A CN103392506A CN 103392506 A CN103392506 A CN 103392506A CN 2013103392607 A CN2013103392607 A CN 2013103392607A CN 201310339260 A CN201310339260 A CN 201310339260A CN 103392506 A CN103392506 A CN 103392506A
- Authority
- CN
- China
- Prior art keywords
- liquid
- mycelium
- ganoderma lucidum
- fermentation
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method. The chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method comprises the following steps that a bacterial strain is transferred to a slope medium to carry out cultivation and activation, and a slope strain is obtained; the slope strain is manufactured into spore suspension to be received into a liquid medium, shake-flask culture is conducted, and a liquid strain is obtained; high-temperature sterilization is conducted on a fermentation medium and then cooling is conducted on the fermentation medium, the liquid strain is received, shake-flask culture is conducted, and fermentation broth of the mycelium is obtained; the mycelium and the fermentation broth are separated through filtering, the mycelium is washed by purified water, the medium is washed away, the mycelium is collected and is baked to be in a constant weight, and a dry mycelium body is obtained. The chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method is characterized in that microelements are added into the fermentation medium, can improve the content of microelements in the ganoderma lucidum mycelium, and is easy to operate, low in cost and suitable for industrial production.
Description
Technical field
The present invention relates to the fungi fermentation engineering, in particular to a kind of production method of rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation.
Background technology
Glossy ganoderma claims again polyporus lucidus, refreshing sesame, sesame grass, celestial grass, auspicious grass, is the complete stool of the red sesame of Polyporaceae plant or purple sesame.Take the Ganoderma Sinense drug effect as best, glossy ganoderma originates in east Asia, distribution in China the widest in Jiangxi, glossy ganoderma is as the China's tradition valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, possesses very high medical value, modern pharmacology through the scientific research institution many decades studies confirm that, glossy ganoderma, for strengthening body immunity, is regulated blood sugar, control blood pressure, auxiliary tumor chemoradiotherapy, liver protecting, the aspects such as promotion sleep all have significant curative effect.
People have carried out a large amount of research to the pharmacological action of glossy ganoderma, confirm that it has pharmacological activity and clinical effect widely.Through a large amount of studies have shown that, the mycelium that Ganoderma lucidum submerged fermentation is produced and the composition fairly similar of natural glossy ganoderma, and proved that it has similar pharmacological action and clinical effectiveness to natural glossy ganoderma,
The glossy ganoderma chemical composition mainly contains ergosterol 0.3%～0.4%, fungal lysozyme and acid protease, L-mannitol.In water-soluble extracting liquid, contain water soluble protein, several amino acids, polypeptide and the polysaccharoses such as asparatate, glutamic acid, arginine, lysine, leucine, alanine, tryptophan, threonine, proline, methionine, phenyl alanine, serine.Still resinous, lactone, coumarin etc.
Purple sesame contains ergosterol, and organic acid is ricinic acid, fumaric acid.In addition, and contain amino acid, glucose, polysaccharose, resin and mannitol.General glossy ganoderma has the reactions such as alkaloid, sterol, lactone, coumarin, acidic resins, amino acid, grease, reducing substances.
The chemical composition of Ganoderma is comparatively complicated, and because of bacterial classification used, the bacterial classification place of production, cultivation method, extraction process, formulation method different different.In the fruit body of Ganoderma, mycelium and spore, contain polysaccharose, ucleosides, furan derivative, steroid ferment class, alkaloids, protein, polypeptide, amino acids, triterpenes, sequiterpene, organic germanium, mineral salt etc.GL-B is one of main active ingredient of glossy ganoderma, has antitumor, immunological regulation, hypoglycemic, anti-oxidant, reducing blood lipid and anti-aging effects.Not lower over one hundred kind of the contained triterpenes of glossy ganoderma, wherein take tetracyclic triterpenes as main, the bitter taste of glossy ganoderma is relevant with contained triterpenes.Triterpenes is also one of active ingredient of glossy ganoderma, and human liver cancer cell is had to cytotoxicity, also can suppress the release of histamine, has hepatoprotective effect and has anti-allergic effects etc.
Human total weight's ten thousand/following element, as zinc, copper, manganese, chromium, selenium, molybdenum, cobalt, fluorine etc., be called trace element.The content of trace element in human body is really very little, as zinc, only accounts for human total weight's 1,000,000/33.
Up to the present, being identified the essential trace element relevant with health and life has 18 kinds, and iron, copper, zinc, cobalt, manganese, chromium, selenium, iodine, nickel, fluorine, molybdenum, vanadium, tin, silicon, strontium, boron, rubidium, arsenic etc. are namely arranged.This every kind trace element has its special physiological function.Although they are minimum at people's in-vivo content, they are very necessary to some conclusive metabolism of remaining in human body.In case lacked these essential trace elements, human body just there will be disease, even threat to life.
It is imperative that thereby the improvement by production technology increases substantially content and the kind of trace element in ganoderma lucidum mycelium.
Summary of the invention
The object of the present invention is to provide a kind of method of rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation, to overcome the low and single defect of micronutrient levels in the prior art ganoderma lucidum mycelium.
After systematic research and test, proposed to improve by adding trace element the production method of the output of Ganoderma lucidum submerged fermentation Chinese caterpillar fungus hypha body weight trace element, method comprises:
(1) bacterial strain is transferred and on slant medium, cultivate, activate, obtain slant strains;
(2) slant strains is made to spore suspension, be connected in liquid nutrient medium, shaking flask is cultivated, and obtains liquid spawn;
(3) cooling after the fermentation medium high-temperature sterilization, then by the liquid spawn access, shaking flask is cultivated, and obtains containing mycelial zymotic fluid;
(4) by filtering mycelium and separation of fermentative broth, the purified rinse water mycelia, wash away medium, collects mycelium, dries to constant weight, and makes dried mycelium.
In the second step of described method, liquid spawn culture medium formula used is micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
In the second step of described method, process conditions used are: the liquid spawn culture medium liquid amount is the 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ° of C, rotating speed 160r/min.
In the 3rd step of described method, culture medium prescription used is micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
In the 3rd step of described method, process conditions used are: liquid amount 200mL/500mL triangular flask, and adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, under 23 ℃, shake the 150r/min rotating speed and cultivate 10d.
The ratio of described trace elements of selenium element, chromium element, zinc element, manganese element and boron element is: 10: 10: 3: 3: 1.Key technology of the present invention is in fermentation medium, adds simultaneously selenium element, chromium element, zinc element, manganese element and boron element.
The present invention has improved the content of trace element in the ganoderma lucidum mycelium, and then improves the drug effect of ganoderma lucidum mycelium, and significant, the mycelia of production can be used as functional food, health products, the raw material of medicine; This method is easy to operate, and cost is low, is suitable for industrial production.
Embodiment
Embodiment 1: bacterial strain is transferred and under on the PDA slant medium 25 ℃, cultivated 7d, activation 2 times, obtain slant strains; Scrape gently and wash slant strains and it is made to spore suspension, then use sterile gauze (6 layers) to filter out the mycelia in suspension, every bottle of moderate Reishi sporule of liquid spawn access concentration, make the mycelium pellet in the liquid of making tiny and even, the zymotic fluid clear.The liquid spawn culture medium formula is: micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8, liquid spawn culture medium liquid amount are the 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ° of C, rotating speed 160r/min, obtain liquid spawn.
Use optimized fermentation medium: micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.Process conditions used are: liquid amount 200mL/500mL triangular flask, and adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, under 23 ℃, shake the 150r/min rotating speed and cultivate 10d.Obtain the zymotic fluid that most of mycelium morphology is mycelium pellet; After filtration by mycelium and separation of fermentative broth, then use the purified rinse water mycelia, after washing away medium, collect mycelium, dry to constant weight.
Claims (6)
1. the method for a rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation, it is characterized in that: the method comprises following four steps: (1) is transferred bacterial strain and is cultivated on slant medium, activates, and obtains slant strains; (2) slant strains is made to spore suspension, be connected in liquid nutrient medium, shaking flask is cultivated, and obtains liquid spawn; (3) cooling after the fermentation medium high-temperature sterilization, then by the liquid spawn access, shaking flask is cultivated, and obtains containing mycelial zymotic fluid; (4) by filtering mycelium and separation of fermentative broth, the purified rinse water mycelia, wash away medium, collects mycelium, dries to constant weight, and makes dried mycelium.Key problem in technology is in fermentation medium, to add trace element.We's Faxian can improve the content of trace element in ganoderma lucidum mycelium.
2. the method for fermenting according to rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid claimed in claim 1, it is characterized in that: in the second step of described method, liquid spawn culture medium formula used is: micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
3. according to the method for rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation claimed in claim 1, it is characterized in that: in the second step of described method, process conditions used are: the liquid spawn culture medium liquid amount is the 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ℃, rotating speed 160r/min.
4. according to the method for rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation claimed in claim 1, it is characterized in that: in the 3rd step of described method, culture medium prescription used is micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH
2PO
40.1-0.2%, water 1000mL, pH7.5-8.
5. the method for fermenting according to rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid claimed in claim 1, it is characterized in that: in the 3rd step of described method, process conditions used are: liquid amount 200mL/500mL triangular flask, adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, under 23 ℃, shake the 150r/min rotating speed and cultivate 10d.
6. according to the method for rich chromium MnZn selenium boron ganoderma lucidum mycelium liquid fermentation claimed in claim 1, it is characterized in that: the ratio of described trace elements of selenium, chromium, zinc, manganese and boron is: 10: 10: 3: 3: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103392607A CN103392506A (en) | 2013-08-02 | 2013-08-02 | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103392607A CN103392506A (en) | 2013-08-02 | 2013-08-02 | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103392506A true CN103392506A (en) | 2013-11-20 |
Family
ID=49556421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103392607A Pending CN103392506A (en) | 2013-08-02 | 2013-08-02 | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103392506A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834138A (en) * | 2017-02-16 | 2017-06-13 | 江苏中祥高科技实业有限公司 | A kind of production method of zinc-rich ganoderma lucidum mycelium |
| CN108522145A (en) * | 2018-05-11 | 2018-09-14 | 福建省农业科学院农业质量标准与检测技术研究所 | A kind of white fungus culture medium and cultivating white fungus method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358413A (en) * | 2000-10-06 | 2002-07-17 | 宋在澫 | Method for preparing mushroom mycelium and use |
| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
| CN102475033A (en) * | 2010-11-24 | 2012-05-30 | 金勇� | Method for producing ganoderma lucidum mycelia rich in selenium |
| CN102972211A (en) * | 2012-12-21 | 2013-03-20 | 徐州工程学院 | Method for increasing growth speed of ganoderma lucidum mycelia and liquid fermentation biomass |
-
2013
- 2013-08-02 CN CN2013103392607A patent/CN103392506A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358413A (en) * | 2000-10-06 | 2002-07-17 | 宋在澫 | Method for preparing mushroom mycelium and use |
| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
| CN102475033A (en) * | 2010-11-24 | 2012-05-30 | 金勇� | Method for producing ganoderma lucidum mycelia rich in selenium |
| CN102972211A (en) * | 2012-12-21 | 2013-03-20 | 徐州工程学院 | Method for increasing growth speed of ganoderma lucidum mycelia and liquid fermentation biomass |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834138A (en) * | 2017-02-16 | 2017-06-13 | 江苏中祥高科技实业有限公司 | A kind of production method of zinc-rich ganoderma lucidum mycelium |
| CN108522145A (en) * | 2018-05-11 | 2018-09-14 | 福建省农业科学院农业质量标准与检测技术研究所 | A kind of white fungus culture medium and cultivating white fungus method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103073651B (en) | Ganoderan extraction method and ganoderan use | |
| CN101942355A (en) | Comprehensive extraction method for extracting tea seed oil, tea saponin and tea seed polysaccharide from tea seeds or camellia seeds | |
| CN105820956B (en) | One plant of Antrodia camphorata bacterial strain and Antrodia camphorata liquid state fermentation method | |
| CN101143002A (en) | Deep liquid fermentation method for preparing selenium-rich gold needle mushroom crude polysaccharide powder | |
| CN102936609B (en) | Preparation method of swift moth paecilomyces varioti extracellular acid glycoprotein | |
| CN105925635A (en) | Production process of icariside II | |
| CN103966105B (en) | One kind conversion ginsenoside Rg3Produce Rh2Aspergillus oryzae, production method and application | |
| KR20130011511A (en) | Fermented ginseng food manufacturing method | |
| CN106148200A (en) | The low lead of a kind of selenium-rich, the culture medium of Cordyceps mycelium of arsenic and inoculation method thereof | |
| CN102172171B (en) | Method for culturing germanium-enriched cordyceps mycelium | |
| CN103392506A (en) | Chromium manganese zinc selenium boron-rich ganoderma lucidum mycelium liquid fermentation method | |
| Zhang et al. | Enhancement of diosgenin production in Dioscorea zingiberensis cell culture by oligosaccharide elicitor from its endophytic fungus Fusarium oxysporum Dzf17 | |
| CN103650920A (en) | Method for fermenting ganoderma lucidum mycelia liquid rich in microelements | |
| CN104164370A (en) | Hericium erinaceus and application thereof | |
| CN108588142B (en) | Method for improving polysaccharide content of ganoderma lucidum mycelia by utilizing fungal polysaccharide and ganoderma lucidum product obtained by method | |
| CN1372002A (en) | Method for producing cordycepin powder and active immne cordyceps sinensis polysaccharide frozen dried powder and products thereof | |
| CN106676032B (en) | Method for increasing pachymic acid yield in poria cocos liquid fermentation mycelium | |
| CN102533900A (en) | Method for extracting lentinan | |
| KR20120053564A (en) | Fermented ginseng with ganoderma lucidum beverage manufacturing method and that beverage | |
| CN104278070A (en) | Method for improving content of ergosterol in liquid fermentation products of phellinus igniarius | |
| CN101280333B (en) | Method for preparing penicillium antibacterial peptide from grey rose penicillium | |
| KR101865292B1 (en) | Culture medium composition comprising mushroom extract for culturing phellinus linteus and culture method of phellinus linteus using thereof | |
| KR20200095208A (en) | Co-culture method of mushroom mycelium | |
| CN1546644A (en) | Production method of Chinese caterpillar fungus powder and active Chinese caterpillar fungus polysaccharide freeze-drying powder for immunity, and product thereof | |
| CN110527702A (en) | Utilize the method for mutagenic obtained Inonotus obliquus bacterial strain production Fuscoporia obliqua polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131120 |